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Ultra-Fast Gradient LC Method for Omeprazole Analysis Using a Monolithic Column: Assay Development, Validation, and Application to the Quality Control of Omeprazole Enteric-Coated Pellets

BORGES, Keyller Bastos; SANCHEZ, Antonio Jose Macias; PUPO, Monica Tallarico; BONATO, Pierina Sueli; COLLADO, Isidro Gonzalez
Fonte: AOAC INT Publicador: AOAC INT
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
457.3089%
A method was optimized for the analysis of omeprazole (OMZ) by ultra-high speed LC with diode array detection using a monolithic Chromolith Fast Gradient RP 18 endcapped column (50 x 2.0 mm id). The analyses were performed at 30 degrees C using a mobile phase consisting of 0.15% (v/v) trifluoroacetic acid (TFA) in water (solvent A) and 0.15% (v/v) TFA in acetonitrile (solvent B) under a linear gradient of 5 to 90% B in 1 min at a flow rate of 1.0 mL/min and detection at 220 nm. Under these conditions, OMZ retention time was approximately 0.74 min. Validation parameters, such as selectivity, linearity, precision, accuracy, and robustness, showed results within the acceptable criteria. The method developed was successfully applied to OMZ enteric-coated pellets, showing that this assay can be used in the pharmaceutical industry for routine QC analysis. Moreover, the analytical conditions established allow for the simultaneous analysis of OMZ metabolites, 5-hydroxyomeprazole and omeprazole sulfone, in the same run, showing that this method can be extended to other matrixes with adequate procedures for sample preparation.; FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paulo; CNPq Conselho Nacional de Desenvolvimento Cientifico e Tecnologico; CAPES Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Junta Andalusia[P07-FQM-02689]

Disposable Graphite Foil Based Electrodes and Their Application in Pharmaceutical Analysis

OLIVEIRA, Andre G.; MUNOZ, Rodrigo Alejandro A.; ANGNES, Lucio
Fonte: WILEY-V C H VERLAG GMBH Publicador: WILEY-V C H VERLAG GMBH
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
563.93582%
This paper explores a new source of graphite for working electrodes, which presents advantages such as low electrical resistance, good flexibility, favorable mechanical performance, versatility to design electrodes in almost any size and very low cost. The new electrodes were investigated in batch electrochemical cells as associated with flow injection analysis systems. Cyclic voltammetry, stripping voltammetry, and amperometry associated with flow injection analysis techniques were applied for the determination of ascorbic acid, zinc and paracetamol in pharmaceutical formulations, respectively. Well-established analytical methods were applied for comparison purposes. The results herein demonstrate the potential of graphite foils as working electrodes in different electroanalytical methods, offering the possibility of producing disposable sensors for routine applications.; CNPq; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); FAPESP; IM2C (Instituto do Milenio de Materiais Complexos); IM2C (Instituto do Milenio de Materiais Complexos)

Análise químico-farmacêutica do fluconazol e especialidade farmacêutica cápsula; Chemical pharmaceutical analysis of fluconazole and its pharmaceutical speciality capsule

Coelho, Helenilze; Matinatti, Audrei Nunes Fernandes; Araújo, Magali Benjamim de; Bergold, Ana Maria; Bueno, Francie
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
POR
Relevância na Pesquisa
458.19086%
O fluconazol, derivado triazólico, apresenta atividade antifúngica sendo indicado no tratamento de grande variedade de infecções fúngicas. Este trabalho foi desenvolvido com o objetivo de estabelecer parâmetros de controle de qualidade para a matériaprima de fluconazol e forma farmacêutica cápsula, subsidiando a elaboração da monografia para a Farmacopéia Brasileira. A matéria-prima do fluconazol pode ser caracterizada pelos seguintes testes: aspecto, solubilidade e faixa de fusão. As impurezas do fluconazol podem ser detectadas por ensaio-limite de cloreto, sulfato, ferro, metais pesados, perda por dessecação e cinzas sulfatadas. Entre as provas de identificação pode-se reconhecer o fármaco por reações químicas de grupos funcionais e por técnicas instrumentais. Para determinação quantitativa do fluconazol na matéria-prima, empregou-se a volumetria com ácido perclórico, em meio acético e detecção do ponto final por indicadores e a espectrofotometria na região do ultravioleta, utilizando hidróxido de sódio 0,1 M a 261 nm. Os parâmetros estabelecidos para o controle de qualidade das amostras de cápsulas dos laboratórios analisados foram aspecto, variação do peso, desintegração, teste de dissolução e perfil de dissolução. O método de doseamento do fluconazol...

Analytical Validation of Quantitative High-Performance Liquid Chromatographic Methods in Pharmaceutical Analysis: A Practical Approach

Bonfilio, Rudy; Laignier Cazedey, Edith Cristina; de Araujo, Magali Benjamim; Salgado, Hérida Regina Nunes
Fonte: Taylor & Francis Inc Publicador: Taylor & Francis Inc
Tipo: Revisão Formato: 87-100
ENG
Relevância na Pesquisa
660.7383%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Analytical validation is an essential component in allowing a laboratory to ensure routine acceptable performance of analytical methods. Despite the considerable amount of important published work on this subject, diversity still prevails in the employed methodologies because validation of an analytical method depends on the specific purpose of that method. This can lead to difficulties in validation approaches and the interpretation of results. Aiming to assist in the planning of validation methods, we discuss relevant approaches of various parameters in quantitative high-performance liquid chromatographic methods and validation fields in pharmaceutical analysis. Moreover, this article provides several up-to-date examples that should be useful as an introduction to analytical validation for practical applications in academic research or the industrial sector.

Recent applications of analytical techniques for quantitative pharmaceutical analysis: A review

Bonfilio, Rudy; de Araújo, Magali Benjamim; Salgado, Hérida Regina Nunes
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 316-338
ENG
Relevância na Pesquisa
670.2446%
The intension of this paper was to review and discuss some of the current quantitative analytical procedures which are used for quality control of pharmaceutical products. The selected papers were organized according to the analytical technique employed. Several techniques like ultraviolet/visible spectrophotometry, fluorimetry, titrimetry, electroanalytical techniques, chromatographic methods (thin-layer chromatography, gas chromatography and high-performance liquid chromatography), capillary electrophoresis and vibrational spectroscopies are the main techniques that have been used for the quantitative analysis of pharmaceutical compounds. In conclusion, although simple techniques such as UV/VIS spectrophotometry and TLC are still extensively employed, HPLC is the most popular instrumental technique used for the analysis of pharmaceuticals. Besides, a review of recent works in the area of pharmaceutical analysis showed a trend in the application of techniques increasingly rapid such as ultra performance liquid chromatography and the use of sensitive and specific detectors as mass spectrometers.

Pharmaceutical marketing and social media : a facebook, twitter and youtube analysis

Costa, Tiago Filipe Tavares
Fonte: Universidade dos Açores Publicador: Universidade dos Açores
Tipo: Dissertação de Mestrado
Publicado em 12/12/2014 ENG
Relevância na Pesquisa
372.43438%
Dissertação de Mestrado em Gestão de Empresas/MBA.; Com o aumento da importância dada aos social media pelo mundo empresarial, os decisores estão cada vez mais interessados nesta temática. As plataformas online como o Facebook, Twitter e YouTube fazem, hoje em dia, parte das estratégias comunicacionais das empresas, provocando um diálogo entre os marketers e os utilizadores/consumidores. Embora estas estratégias comunicacionais estejam a ser usadas na indústria farmacêutica, esta indústria, e especialmente o seu departamento de marketing, encontra-se sob altas pressões regulamentares devido à natureza do seu negócio que podem suprimir a adesão rápida aos social media. Também devido à falta de regulação oficial e específica dirigida para os social media, torna-se difícil, ao nível do marketing farmacêutico, delinear uma estratégia comunicacional nesta área. Esta investigação tem como objetivo avaliar a presença da indústria farmacêutica nos social media, nomeadamente no Facebook, Twitter e YouTube, e ao mesmo tempo caracterizar as diferentes estratégias para o engagement digital. De forma a concretizar esta investigação foi desenvolvida uma abordagem metodológica específica e um modelo concetual que permitiu avaliar a presença nos social media selecionados...

Spectrophotometric determination of mosapride in pure and pharmaceutical preparations

Revanasiddappa,H. D.; Veena,M. A.
Fonte: Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP Publicador: Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2007 EN
Relevância na Pesquisa
454.77926%
Two simple and sensitive spectrophotometric methods (M1 and M2) for the determination of mosapride in pure and in pharmaceutical preparations are described. These methods are based on the interaction of diazotized mosapride (MSP) couples with chromotropic acid (CTA) [M1] in alkaline medium and diphenylamine (DPA) [M2] in acidic medium. The resulting azo-dyes exhibit maximum absorption at 560 nm and at 540 nm for methods M1 and M2, respectively. All variables were studied in order to optimize the reaction conditions. No interferences were observed from excipients, and the validity of the each method was tested against reference method.

Spectrophotometric flow injection procedure to indirect determination of paracetamol in pharmaceutical formulations using o-tolidine as reagent

Fatibello-Filho,O.; Vieira,H. J.
Fonte: Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP Publicador: Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2008 EN
Relevância na Pesquisa
462.1015%
A spectrophotometric flow injection method for the determination of paracetamol in pharmaceutical formulations is proposed. The procedure was based on the oxidation of paracetamol by sodium hypochloride and the determination of the excess of this oxidant using o-tolidine dichloride as chromogenic reagent at 430 nm. The analytical curve was linear in the paracetamol concentration range from 8.50 x 10-6 to 2.51 x 10-4 mol L-1 with a detection limit of 5.0 x 10-6 mol L-1. The relative standard deviation was smaller than 1.2% for 1.20 x 10-4 mol L-1 paracetamol solution (n = 10). The results obtained for paracetamol in pharmaceutical formulations using the proposed flow injection method and those obtained using a USP Pharmacopoeia method are in agreement at the 95% confidence level.

Arsenic determination in pharmaceutical grade barium sulfate using direct solid sampling electrothermal atomic absorption spectrometry

Bolzan,Rodrigo C.; Moraes,Diogo P. de; Mattos,Julio Cezar P. de; Dressler,Valderi L.; Flores,Érico M. de Moraes
Fonte: Sociedade Brasileira de Química Publicador: Sociedade Brasileira de Química
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2010 EN
Relevância na Pesquisa
454.77926%
A method for arsenic determination in pharmaceutical grade BaSO4 by solid sampling electrothermal atomic absorption spectrometry (SoS-ET AAS) is described. Two background (BG) correction systems, deuterium and Zeeman-effect (Z), were evaluated. Background was decreased by using hydrogen combined with Pd. Accurate and precise results were obtained using Z-SoS-ET AAS. Relative standard deviation using Z-SoS-ET AAS was lower than 9.5%. Limit of detection was 0.005 μg g-1 and characteristic mass was 25.4 pg. Results were also compared with those obtained using the preparation method suggested by European Pharmacopoeia for BaSO4 followed by determination using inductively coupled plasma optical emission spectrometry and inductively coupled plasma mass spectrometry. Results were around 20% lower than results obtained using the proposed method. Some advantages as simplicity, higher sample throughput were found when compared with the official method currently described in pharmacopoeia.

Validated microbiological and HPLC methods for the determination of moxifloxacin in pharmaceutical preparations and human plasma

Abdelaziz,Ahmed A.; Elbanna,Tarek E.; Gamaleldeen,Noha M.
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2012 EN
Relevância na Pesquisa
455.1944%
The article presents a comparison between microbiological and high performance liquid chromatographic (HPLC) assays for quantification of moxifloxacin in tablets, ophthalmic solutions and human plasma. The microbiological method employed a cylinder-plate agar diffusion assay using a strain of Esherichia coli ATCC 25922 as the test organism and phosphate buffer (pH8) as the diluent. The calibration curves were linear (R²> 0.98) over a concentration range of 0.125 to 16 µgml-1. The within day and between days precisions were < 4.47% and < 6.39% respectively. Recovery values were between 89.4 and 110.2%. The HPLC assay used Hypersil® BDS C18 reversed phase column (250×4.6 mm, 5µm) with a mobile phase comprising 20 mM ammonium dihydrogen orthophosphate (pH3) and acetonitrile (75:25) and flowing at 1.5 ml/min. The detection was at 295nm. The calibration curves were linear (R²> 0.999) over the range of 0.125 to 16 µg ml-1. The within day and between days precisions were < 4.07% and < 5.09% respectively. Recovery values were between 97.7 and 107.6%. Similar potencies were obtained after the analysis of moxifloxacin tablets and ophthalmic solutions by both methods. Also pharmacokinetic parameters were calculated after the analysis of plasma samples of six male healthhy volunteers by both validated methods.

Electrochemical biosensors in pharmaceutical analysis

Gil,Eric de Souza; Melo,Giselle Rodrigues de
Fonte: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Publicador: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2010 EN
Relevância na Pesquisa
668.5755%
Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors.

Automation in the pharmaceutical analysis laboratory: a centralized/decentralized approach

Scypinski, Stephen; Nelson, Linda; Sadlowski, Theodore
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Publicado em //1995 EN
Relevância na Pesquisa
453.14598%
It has been over 10 years since robots have appeared in the pharmaceutical analysis laboratory. In the early days, it was common for one selected individual to be responsible for the programming, usage and maintenance of the robots(s). However, the increasing use of robotics has prompted the formation of robotics ‘laboratories’ and/or ‘groups’. This is especially true when multiple robotic systems and applications are involved.

Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent Advances

Darwish, Ibrahim A.
Fonte: Master Publishing Group Publicador: Master Publishing Group
Tipo: Artigo de Revista Científica
Publicado em /09/2006 EN
Relevância na Pesquisa
471.32766%
Immunoassays are bioanalytical methods in which the quantitation of the analyte depends on the reaction of an antigen (analyte) and an antibody. Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and bioequivalence studies in drug discovery and pharmaceutical industries. The importance and widespread of immunoassay methods in pharmaceutical analysis are attributed to their inherent specificity, high-throughput, and high sensitivity for the analysis of wide range of analytes in biological samples. Recently, marked improvements were achieved in the field of immunoassay development for the purposes of pharmaceutical analysis. These improvements involved the preparation of the unique immunoanalytical reagents, analysis of new categories of compounds, methodology, and instrumentation. The basic methodologies and recent advances in immunoassay methods applied in different fields of pharmaceutical analysis have been reviewed.

Principles of Micellar Electrokinetic Capillary Chromatography Applied in Pharmaceutical Analysis

Hancu, Gabriel; Simon, Brigitta; Rusu, Aura; Mircia, Eleonora; Gyéresi, Árpád
Fonte: Tabriz University of Medical Sciences Publicador: Tabriz University of Medical Sciences
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
464.1499%
Since its introduction capillary electrophoresis has shown great potential in areas where electrophoretic techniques have rarely been used before, including here the analysis of pharmaceutical substances. The large majority of pharmaceutical substances are neutral from electrophoretic point of view, consequently separations by the classic capillary zone electrophoresis; where separation is based on the differences between the own electrophoretic mobilities of the analytes; are hard to achieve. Micellar electrokinetic capillary chromatography, a hybrid method that combines chromatographic and electrophoretic separation principles, extends the applicability of capillary electrophoretic methods to neutral analytes. In micellar electrokinetic capillary chromatography, surfactants are added to the buffer solution in concentration above their critical micellar concentrations, consequently micelles are formed; micelles that undergo electrophoretic migration like any other charged particle. The separation is based on the differential partitioning of an analyte between the two-phase system: the mobile aqueous phase and micellar pseudostationary phase. The present paper aims to summarize the basic aspects regarding separation principles and practical applications of micellar electrokinetic capillary chromatography...

Multiresidue method for the analysis of herbicides in surface and groundwater.

SANTOS, N.A.G.; BONATO, P.S.; DREOSSI, S.A.C.; GOMES, M.A.; CERDEIRA, A. L.; CARVALHO, D.; LANCHOTE, V.L.
Fonte: In: CONGRESS OF PHARMACEUTICAL SCIENCES, 1., 1997, Ribeirao Preto, SP. Bolletino Chimico Farmaceutico, v.136, p.133, 1997. Abstract. Publicador: In: CONGRESS OF PHARMACEUTICAL SCIENCES, 1., 1997, Ribeirao Preto, SP. Bolletino Chimico Farmaceutico, v.136, p.133, 1997. Abstract.
Tipo: Resumo em anais de congresso (ALICE)
EN
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446.72516%
The high standards for drinking water purity laid down by the European Community, United States and other coutries require the development of suitable analytical methods with high sensitivity, selectivity, accuracy and reliability, that permit the analysis of a broad variety of substances in a single run, or at least those substances that are important in a specific water catchment area. The standard method for the investigation of environmental pollutants has been gas chromatography using different selective detectors after extraction with different organic solvents. Some of that substances that are difficult to analyze by gas chromatography because of thermal instability or chemical polarity, may be analyzed by high performance liquid chromatography. In the present study we employed this technique to develop a highly sensitive method for the simultaneous analysis of atrazine, simazine, ametryne, tebuthiuron and diuron in surface and groundwater. The aqueous samples (100 ml ) were filtered through 0.22 um membranes to remove suspended matter. After adjusting the pH by the addition of 25 uL of 4M NaOH, the samples were extracted with 12 ml dichloromethane by mechanical shaking for 1 hour. After phase separation, 6 ml of the organic phases were transferred to conic test tubes and the solvent was evaporated to dryness under a stream of nitrogen at 35oC. The residues were dissolved in 200 ul of the mobile phase and 100 ul were chromatographed on a Lichrospher 100 RP-8 column (particle 5 um ...

Fluorimetria na análise farmacêutica: uma revisão; Fluorimetry in pharmaceutical analysis: a review

Santos, Diego Ives de Vilasboas e; Gil, Eric de Souza
Fonte: Ricardo Menegatti Publicador: Ricardo Menegatti
Tipo: Artigo de Revista Científica
OTHER
Relevância na Pesquisa
466.26438%
v.7,n1,p.12-26.ja./mar. 2010.; Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Federal de Goiás.; A fluorimetria é um método de análise usado na determinação quantitativa ou qualitativa de substâncias que são capazes de emitir fluorescência. Sendo assim, pode ser muito útil na análise farmacêutica, para doseamento de fármacos em formulações farmacêuticas, identificação de impurezas, estudos de interações ligante-receptor, e até mesmo quantificação. Aparelhos usados em fluorimetria podem ser usados também como sistemas de detecção em outros métodos, como cromatografia líquida de alta eficiência (CLAE) e cromatografia gasosa. Sendo assim, este artigo de revisão traz várias aplicações possíveis para a fluorimetria dentro da análise farmacêutica, com trabalhos que relatam o uso da fluorimetria na análise de fármacos.; Fluorimetric method of analysis is used in qualitative or quantitative determination of substances which are capable of delivering fluorescence. Thus, it can be very useful in pharmaceutical analysis for determination of drugs in pharmaceutical formulations, identification of impurities, studies of ligand-receptor interactions, and even quantification. Equipment used in fluorimetry can be used as detection systems in other methods...

Einsatz der NIR Spektroskopie in der pharmazeutischen Analytik : Charakterisierung von Johanniskraut-Trockenextrakten und Kopplungsmöglichkeiten mit der HPTLC; Use of NIR Spectroscopy in pharmaceutical analysis : Characterization of St. John's Wort Extracts and possibilities of coupling with HPTLC

Rager, Irene Cora Ottilia
Fonte: Universität Tübingen Publicador: Universität Tübingen
Tipo: Dissertation; info:eu-repo/semantics/doctoralThesis
DE_DE
Relevância na Pesquisa
558.6899%
In der vorliegenden Arbeit wurde mit der Nahinfrarot Spektroskopie ein in der pharmazeutischen Industrie relativ neues Verfahren eingesetzt, welches seinen Ursprung in der Lebensmittelchemie und Analytik von Agrarprodukten hat. Die vorliegende Arbeit untersucht neue Grenzen dieser Routineanalytik, indem sie beweist, dass bisher nicht erreichte Nachweis- und Bestimmungsgrenzen in komplexen Matrices – Pflanzenextrakten und HPTLC-Sorbentien – dieser spektroskopischen Methode durchaus zur Verfügung stehen. Damit eröffnet sie neue Anwendungsmöglichkeiten dieser Routineanalytik für die pharmazeutische Qualitätskontrolle und diskutiert ihre Einsatzgebiete.; This thesis shows two new applications for the well-known Near Infrared Reflectance Spectoscopy (NIRS). In order to establish new methods for this routine technique the quanitfication and identification possiblities in very complex matrices are investigated. First, two major consitutents of St. John's Wort extracts have been quantified by direct measurement of the extracts via NIR spectroscopy. The second part shows a new method of coupling NIRS with thin-layer chormatography with its possiblities and limitations for quanitfication and identification of pharmaceutical compounds.

Spectrophotometric determination of metronidazole through Schiff's base system using vanillin and PDAB reagents in pharmaceutical preparations

Siddappa,K.; Mallikarjun,M.; Reddy,P. T.; Tambe,M.
Fonte: Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP Publicador: Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2008 EN
Relevância na Pesquisa
453.14598%
Two simple sensitive and reproducible spectrophotometric methods have been developed for the determination of metronidazole either in pure form or in their tablets. The proposed methods are based on the reduction of the nitro group to amino group of the drug. The reduction of metronidazole was carried out with zinc powder and 5 N hydrochloric acid at room temperature in methanol. The resulting amine was then subjected to a condensation reaction with aromatic aldehyde namely, vanillin and p-dimethyl amino benzaldehyde (PDAB) to yield yellow colored Schiff's bases. The formed Schiff's bases are quantified spectrophotometrically at their absorption maxima at 422 nm for vanillin and 494 nm for PDAB. Beer's law was obeyed in the concentration ranges 10 to 65 µg mL-1 and 5 to 40 µg mL-1 with a limit of detection (LOD) of 0.080 µg mL-1 and 0.090 µg mL-1 for vanillin and PDAB, respectively. The mean percentage recoveries were found to be 100.05 ± 0.37 and 99.01 ± 0.76 for the two methods respectively. The proposed methods were successfully applied to determine the metronidazole in their tablet formulations and the results compared favorably to that of reference methods. The proposed methods are recommended for quality control and routine analysis.

Electrochemical biosensors in pharmaceutical analysis

Gil, Eric de Souza; Melo, Giselle Rodrigues de
Fonte: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas Publicador: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/09/2010 ENG
Relevância na Pesquisa
668.5755%
Em virtude do aumento da demanda por técnicas analíticas simples e de baixo custo, os biossensores têm atraído a atenção para a análise de fármacos, medicamentos e outros analitos de interesse em controle de qualidade de medicamentos. Os biossensores permitem a quantificação não somente de princípio ativo em formulações farmacêuticas, mas também de produtos de degradação e metabólitos em fluídos biológicos, bem como análise de amostras de interesse clínico e industrial, além de possibilitar a determinação de enantiômeros. Desta forma, este artigo objetiva fazer uma breve revisão a respeito do emprego de biossensores em análise farmacêutica, com ênfase em sensores eletroquímicos enzimáticos.; Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors.

Análise químico-farmacêutica do fluconazol e especialidade farmacêutica cápsula; Chemical pharmaceutical analysis of fluconazole and its pharmaceutical speciality capsule

Coelho, Helenilze; Matinatti, Audrei Nunes Fernandes; Araújo, Magali Benjamim de; Bergold, Ana Maria; Bueno, Francie
Fonte: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas Publicador: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Artigo Avaliado pelos Pares Formato: application/pdf
Publicado em 01/06/2004 POR
Relevância na Pesquisa
458.19086%
O fluconazol, derivado triazólico, apresenta atividade antifúngica sendo indicado no tratamento de grande variedade de infecções fúngicas. Este trabalho foi desenvolvido com o objetivo de estabelecer parâmetros de controle de qualidade para a matéria-prima de fluconazol e forma farmacêutica cápsula, subsidiando a elaboração da monografia para a Farmacopéia Brasileira. A matéria-prima do fluconazol pode ser caracterizada pelos seguintes testes: aspecto, solubilidade e faixa de fusão. As impurezas do fluconazol podem ser detectadas por ensaio-limite de cloreto, sulfato, ferro, metais pesados, perda por dessecação e cinzas sulfatadas. Entre as provas de identificação pode-se reconhecer o fármaco por reações químicas de grupos funcionais e por técnicas instrumentais. Para determinação quantitativa do fluconazol na matéria-prima, empregou-se a volumetria com ácido perclórico, em meio acético e detecção do ponto final por indicadores e a espectrofotometria na região do ultravioleta, utilizando hidróxido de sódio 0,1 M a 261 nm. Os parâmetros estabelecidos para o controle de qualidade das amostras de cápsulas dos laboratórios analisados foram aspecto, variação do peso, desintegração, teste de dissolução e perfil de dissolução. O método de doseamento do fluconazol...