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Three in one: fixing marine nematodes for ecological, molecular, and morphological studies

Fonseca, Gustavo Fernandes Camargo; Ale, Karin Hoch Fehlauer
Fonte: AMER SOC LIMNOLOGY OCEANOGRAPHY; WACO Publicador: AMER SOC LIMNOLOGY OCEANOGRAPHY; WACO
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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Multidisciplinary benthic studies are still hindered by the lack of a unique fixative that satisfactorily preserves morphology and DNA, and that is simultaneously adequate for ecological surveys. The objective of this study is to test the performance of five fixatives: formalin, ethanol, dimethylsulfoxide with EDTA and NaCl salts (DESS), methanol with acetic acid (METHAC), and ethanol with acetic acid (ETHAC), for the preservation of estuarine and exclusively marine nematode assemblages for morphological, molecular, and ecological studies. The presence of the stain rose bengal in each fixative was also evaluated in the yield of PCR reactions. For molecular analyses, one species of each habitat was considered. Results revealed that fixative performance for morphological studies is habitat-and species-dependent. For studies of estuarine sediment nematodes, we recommend the use of pure ethanol, because it caused little morphological distortion (<10% of the assemblage), preserved all the species for ecological studies, and yielded high quality DNA sequences. For studies of exclusively marine environments, METHAC or DESS are the most adequate. The first performed better for morphological and ecological surveys, whereas the second was more appropriate for molecular research. For ecological studies...

Photophysical properties and interactions of xanthene dyes in aqueous micelles

Pellosi, Diogo Silva; Estevao, Bianca Martins; Semensato, Juliana; Severino, Divinomar; Baptista, Mauricio da Silva; Politi, Mario Jose; Hioka, Noboru; Caetano, Wilker
Fonte: ELSEVIER SCIENCE SA; LAUSANNE Publicador: ELSEVIER SCIENCE SA; LAUSANNE
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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Photosensitizers (PS) photodynamic activities are regulated by their location in the biological target, which modulates their photophysical and photochemical features. In this work the PS partition for the Xanthene Dyes Fluorescein (FSC), Eosin Y(EOS), Erythrosin B (ERY) and Rose Bengal B (RBB) in biomimetic models (SDS, CTAB and Pluronic P-123 micelles) and the effects on their photophysical characteristics are evaluated. The hydrophobic and electrostatic forces that govern the PS-micelle interaction are analyzed. At physiological pH (7.25), the ability of the dianionic protolytic form of the dyes to be positioned into the micelle palisade and its micelle interaction depends not only on the hydrophobicity of the dye but also on the micellar surface charge. The Binding Constants obey exactly the same order of the Partition Coefficients for the dyes in P-123 and CTAB micelles. The Stern-Volmer treatment pointed out that dyes are located inside the micelle, especially ERY and RBB. The magnitude of the dye-micelle interaction increased from SDS, P-123 and finally CTAB micelles due to the charges between dye and micelle, and among the xanthenes, their hydrophobic characteristics. Within the micelle pseudo phase, ERY and RBB are still very efficient photosensitizers exhibiting high quantum yield of singlet oxygen...

The effects of rose bengal- and erythrosine-mediated photodynamic therapy on Candida albicans

Borges Pereira Costa, Anna Carolina; Campos Rasteiro, Vanessa Maria; Pereira, Cristiane Aparecida; Rossoni, Rodnei Dennis; Junqueira, Juliana Campos; Cardoso Jorge, Antonio Olavo
Fonte: Wiley-Blackwell Publicador: Wiley-Blackwell
Tipo: Artigo de Revista Científica Formato: 56-63
ENG
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); The aim of this study was to evaluate the effects of photodynamic therapy (PDT) using rose bengal or erythrosine with light emitting diode (LED) on Candida albicans planktonic cultures and biofilms. Seven C. albicans clinical strains and one standard strain (ATCC 18804) were used. Planktonic cultures and biofilms of each C. albicans strain were submitted to the following experimental conditions: (a) treatment with rose bengal and LED (RB+L+); (b) treatment with erythrosine and LED (E+L+); and (c) control group, without LED irradiation or photosensitiser treatment (P-L-). After irradiation of the planktonic cultures and biofilms, the cultures were seeded onto Sabouraud dextrose agar (37 degrees C at 48 h) for counting of colony-forming units (CFU ml-1) followed by posterior anova and Tukeys test analyses (P < 0.05). The biofilms were analysed using scanning electron microscopy (SEM). The results revealed a significant reduction of planktonic cultures (3.45 log10 and 1.97 log10) and of biofilms (<1 log10) for cultures that were subjected to PDT mediated using either erythrosine or rose bengal, respectively. The SEM data revealed that the PDT was effective in reducing and destroying of C. albicans blastoconidia and hyphae. The results show that erythrosine- and rose bengal-mediated PDT with LED irradiation is effective in treating C. albicans.

Comparison of the efficacy of Rose Bengal and erythrosin in photodynamic therapy against Enterobacteriaceae

Rossoni, Rodnei D.; Junqueira, Juliana Campos; Santos, Evelyn Luzia S.; Costa, Anna Carolina B.; Jorge, Antonio Olavo C.
Fonte: Springer London Ltd Publicador: Springer London Ltd
Tipo: Artigo de Revista Científica Formato: 581-586
ENG
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 07/54442-3; The aim of this study was to evaluate the effects of the photosensitizers Rose Bengal and erythrosin combined with a light-emitting diode (LED) on Enterobacteriaceae. Twelve Enterobacteriaceae strains isolated from the oral cavities of patients undergoing prolonged antibiotic therapy, including three Escherichia coli, three Enterobacter cloacae, three Klebsiella oxytoca and three Klebsiella pneumoniae, were studied. An Enterobacteriaceae suspension (10(6) cells/ml) was prepared from each clinical strain isolated from the human oral cavity and subjected to the following treatments: LED and Rose Bengal, LED and erythrosin, LED and physiological solution, and physiological solution only as control. A blue LED unit (460 nm), and Rose Bengal and erythrosin at a concentration of 50 A mu mol/l were used. After incubation at 37A degrees C for 48 h, the number of colony-forming units (CFU) was calculated and subjected to analysis of variance (ANOVA). The Enterobacterial strains were sensitive to photodynamic therapy with Rose Bengal. There was a reduction of approximately 7.14 log10 for Enterobacter cloacae, 7.73 log10 for Escherichia coli, 6.76 log10 for Klebsiella pneumoniae and 7.21 log10 for Klebsiella oxytoca. However...

Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode

da Costa, Anna Carolina Borges Pereira; Chibebe Junior, José; Pereira, Cristiane Aparecida; Machado, Ana Karina da Silva; Beltrame Junior, Milton; Junqueira, Juliana Campos; Jorge, Antonio Olavo Cardoso
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 413-418
ENG
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The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10 6 cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p ≤ 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log 10 in the RB+L+ group and of 5.16 log 10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied.

Impact of wildfire smoke in Buenos Aires, Argentina, on ocular surface

Berra,Martin; Galperín,Gustavo; Dawidowski,Laura; Tau,Julia; Márquez,Isabel; Berra,Alejandro
Fonte: Conselho Brasileiro de Oftalmologia Publicador: Conselho Brasileiro de Oftalmologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2015 EN
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Purpose: To evaluate the acute impact of the wildfire smoke episode in 2008 on the ocular surface of subjects living in the Metropolitan Area of Buenos Aires (MABA). Methods: A total of 86 subjects were evaluated: Group 1 comprised patients from a public ophthalmology hospital (N=35) and Group 2 comprised healthy volunteers (N=51). All subjects answered a questionnaire on ocular symptoms and underwent ophthalmologic examination [bulbar conjunctival hyperemia, corneal fluorescein staining, rose bengal vital staining, tear break-up time (TBUT), Schirmer I test, tear lysozyme, and impression cytology] during and after the acute episode. Concentrations of carbon monoxide (CO), nitrogen dioxide (NO2), and particulate matter (PM) were measured before, during, and after the acute episode. Results: Both groups showed a statically significant increase in ocular symptoms and bulbar conjunctival hyperemia and a statically significant decrease in tear break-up time during the acute episode. Group 1 showed more severe symptoms and a statistically significant increase in fluorescein and rose bengal staining intensities during the acute episode. We found a significant negative correlation between ocular symptoms and tear break-up time. During the episode...

Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode

Costa,Anna Carolina Borges Pereira a; Chibebe Junior,José; Pereira,Cristiane Aparecida; Machado,Ana Karina da Silva; Beltrame Junior,Milton; Junqueira,Juliana Campos; Jorge,Antonio Olavo Cardoso
Fonte: Sociedade Brasileira de Pesquisa Odontológica - SBPqO Publicador: Sociedade Brasileira de Pesquisa Odontológica - SBPqO
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2010 EN
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48.816045%
The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10(6) cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p < 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log10 in the RB+L+ group and of 5.16 log10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied.

Photodynamic inactivation of infectivity of human immunodeficiency virus and other enveloped viruses using hypericin and rose bengal: inhibition of fusion and syncytia formation.

Lenard, J; Rabson, A; Vanderoef, R
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 01/01/1993 EN
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The mechanism of the antiviral activity of hypericin was characterized and compared with that of rose bengal. Both compounds inactivate enveloped (but not unenveloped) viruses upon illumination by visible light. Human immunodeficiency and vesicular stomatitis viruses were photodynamically inactivated by both dyes at nanomolar concentrations. Photodynamic inactivation of fusion (hemolysis) by vesicular stomatitis, influenza, and Sendai viruses was induced by both dyes under similar conditions (e.g., I50 = 20-50 nM for vesicular stomatitis virus), suggesting that loss of infectivity resulted from inactivation of fusion. Syncytium formation, between cells activated to express human immunodeficiency virus gp120 on their surfaces and CD4+ cells, was inhibited by illumination in the presence of 1 microM hypericin. Hypericin and rose bengal thus exert similar virucidal effects. Both presumably act by the same mechanism--namely, the inactivation of the viral fusion function by singlet oxygen produced upon illumination. The implications of this photodynamic antiviral action for the potential therapeutic usefulness of both hypericin and rose bengal are discussed.

Dichloran-rose bengal medium for enumeration and isolation of molds from foods.

King, A D; Hocking, A D; Pitt, J I
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1979 EN
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Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. Dichloran (2,6 dichloro-4-nitroaniline) restricted growth of Rhizopus stolonifer while allowing satisfactory growth of the other test molds. Three Rhizopus and Mucor species were encountered that were not inhibited by dichloran; these were controlled by the addition of rose bengal. The optimal medium, designated DRBC, contained 2 micrograms of dichloran and 25 micrograms of rose bengal per ml. DRBC, in pure culture tests and with food samples, restricted the colony size of spreading molds and recovered a wider range of species in higher numbers than other enumeration media.

Rose Bengal plate agglutination and counterimmunoelectrophoresis tests on spinal fluid in the diagnosis of Brucella meningitis.

Díaz, R; Maraví-Poma, E; Delgado, G; Rivero, A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1978 EN
Relevância na Pesquisa
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Rose Bengal and counterimmunoelectrophoresis, two tests that detect antibodies against different structural antigens, when carried out on spinal fluid permitted rapid diagnosis of human Brucella meningitis. The Rose Bengal test was positive in five out of five patients studied, and counterimmunoelectrophoresis was positive in all but one. The Brucella meningitis was characterized by an increase of immunoglobulin G in the cerebrospinal fluid.

Albumin-mediated transport of rose bengal by perfused rat liver. Kinetics of the reaction at the cell surface.

Forker, E L; Luxon, B A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1983 EN
Relevância na Pesquisa
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Rapid dissociation of organic anions from plasma albumin maximizes the presentation of free ligand to the cell surface and thus favors its efficient hepatic extraction. Even assuming these optimal conditions, however, taurocholate and rose bengal have hepatic extraction fractions that are higher than can be accounted for by spontaneous dissociation of their albumin-ligand complexes. In this study we developed a transport model that attributes this behavior to sites on the hepatocyte plasma membrane that bind the albumin-ligand complexes, promoting the transport of ligand into the hepatocyte. Fitting this model to rose bengal removal rates measured over a wide range of albumin concentrations yields estimates of the number of cell surface sites and their affinity for albumin. These estimates are in good agreement with those reported by Weisiger, Gollan, and Ockner for the binding of ligand-free albumin to isolated hepatocytes. We conclude that both experiments measure the same phenomenon and, accordingly, that the binding of albumin to the cell surface is the functional equivalent of albumin-mediated transport.

Effects of rose bengal on bile secretion in the rabbit: inhibition of a bile salt-independent fraction

Dhumeaux, D.; Erlinger, S.; Benhamou, J.-P.; Fauvert, R.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1970 EN
Relevância na Pesquisa
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The authors have studied the influence of rose bengal, and, by comparison, of uranin, on choleresis in the rabbit. Uranin induced an increase in bile flow and a decrease in bile salt concentration in bile. These results are consistent with an osmotic mechanism, as proposed by Sperber (1959). By contrast, rose bengal induced a marked decrease in bile flow and an increase in bile salt concentration. In rose bengal-treated animals (30 μmoles per kilogram body weight intravenously), bile flow was 65% lower and bile salt concentration 95% higher than in control animals studied during the same periods. The relationship between bile flow and bile salt excretion is consistent in the rabbit, with the existence of a bile salt-independent secretion of 60 mg per minute per kilogram body weight, that is, 60% of bile flow. In rose bengal-treated animals, this fraction was only 15 mg per minute per kilogram body weight. It is concluded that rose bengal inhibits the bile salt-independent fraction.

Quantitative liver imaging using 131-I Rose Bengal as an index of liver function and prognosis.

Gamlen, T R; Triger, D R; Ackery, D M; Fleming, J S; Grant, R W; Kenny, R W; Maciver, A G; Wright, R
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/1975 EN
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A technique for assessing quantitatively hepatic function by direct measurement of liver parenchymal cell uptake of 131I Rose Bengal using a scintillation camera with a digital store and retrieval system is described. Ninety-four studies were performed on 84 patients with a variety of hepatic disorders over a two-year period, the diagnosis in each case being established by liver biopsy or laparotomy. The results were compared with the clinical, biochemical and histological assessment of the patients. A good correlation was found between the half-time for hepatic uptake of 131I Rose Bengal and the histological changes, as well as with clinical prognosis measured in terms of clinical improvement or deterioration to death. The rate of liver uptake was found to be a better index than the clearance of radioisotope from the blood and was superior to conventional biochemical investigations in both icteric and anicteric patients. The test was not shown to be of clinical value in discriminating between intra- and extrahepatic causes of jaundice. It is suggested that this technique may provide a safe and sensitive method for assessing the severity of liver dysfunction and also for monitoring clinical progress, especially in situations where liver biopsy may be unreliable or hazardous.

Study of the plate agglutination test with rose bengal antigen for the diagnosis of human brucellosis

Černyševa, M. I.; Knjazeva, E. N.; Egorova, L. S.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em //1977 EN
Relevância na Pesquisa
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This study presents the results of a number of serological tests, in particular the plate agglutination test using acid antigen stained with rose bengal, in the examination of 440 human sera for brucellosis. In addition, the sera were examined for the presence of specific antibodies of various classes (IgM and IgG). These investigations showed the rose bengal plate agglutination test to be highly specific and sensitive as a rapid method for the diagnosis of human brucellosis.

Sonodynamic Excitation of Rose Bengal for Eradication of Gram-Positive and Gram-Negative Bacteria

Nakonechny, Faina; Nisnevitch, Michael; Nitzan, Yeshayahu; Nisnevitch, Marina
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
EN
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Photodynamic antimicrobial chemotherapy based on photosensitizers activated by illumination is limited by poor penetration of visible light through skin and tissues. In order to overcome this problem, Rose Bengal was excited in the dark by 28 kHz ultrasound and was applied for inactivation of bacteria. It is demonstrated, for the first time, that the sonodynamic technique is effective for eradication of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The net sonodynamic effect was calculated as a 3-4 log10 reduction in bacteria concentration, depending on the cell and the Rose Bengal concentration and the treatment time. Sonodynamic treatment may become a novel and effective form of antimicrobial therapy and can be used for low-temperature sterilization of medical instruments and surgical accessories.

Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination

Asgher, Muhammad; Yaqoob, Mohammad; Nabi, Abdul; Murtaza, Ghulam; Siddiqi, Abdul Rauf; Waseem, Amir
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
EN
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Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II) for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB•−) which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2•−) and hydrogen peroxide (H2O2). Highly reactive hydroxyl radicals (•OH) were produced via dismutation of H2O2 by catalyst (Cu2+). The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence. The limit of detection (3s of the blank, n = 6) of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05 μg mL−1, respectively. The sample throughput of 70 h−1 for vitamins A and C and 30 h−1 for RB was found. Calibration curve was linear in the range of 0.05–15, 0.01–20, and 0.1–50 μg mL−1 for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; n = 3) in the range 1.6–3.6%. The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values.

Optical and photodynamic properties of higher-lying states of rose bengal

Larkin, John Michael (1970 - ); Foster, Thomas H.
Fonte: University of Rochester Publicador: University of Rochester
Tipo: Tese de Doutorado Formato: Number of Pages:xvii, 195 leaves
ENG
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Thesis (Ph. D.)--University of Rochester. Dept. of Physics and Astronomy, 2000.; We have studied the photophysics and photochemistry of the higher-lying triplet states of rose bengal (tetraiodo-tetrachlorofluorescein) in water.

Triplet-triplet absorption spectroscopy is used to identify a previously undescribed triplet state of rose bengal with an absorption cross section of (1.1 ± 0.1) × 10−16 cm2 at 1064 nm. This state is the least energetic triplet state, excluding T1, to be identified, so it will be denoted as T2.

Two-step excitation techniques for measuring reverse intersystem crossing, an intersystem crossing from a higher-lying triplet state to a singlet state, are described. The method of two-step laser-induced fluorescence is used to determine the quantum yield of reverse intersystem crossing for three higher-lying triplet states of rose bengal. Reverse intersystem crossing yields of 0.0076 ± 0.0002, <0.06, and 0.12 ± 0.02 are found for the triplet states excited by 1064-, 632-, 532-nm light, denoted respectively as T2, T3 and T4. Bond cleavage may also follow excitation of a higher-lying state. Other workers have found evidence for this photochemical process from T3 but not T4. The method of two-step laser-induced bleaching was used to determine whether bond cleavage occurs from T2. No evidence was found for a such a process. It had been hypothesized that reverse intersystem crossing was responsible for the lack of bond cleavage from T4. A theoretical investigation based on kinetic models including reverse intersystem crossing shows that variations in the yield of this process would produce only small changes in the yield of photoproducts formed by bond cleavage.

A two-photon absorption cross section of 0.028 ± 0.001 GM for rose bengal excited by 1064-nm light is determined using a fluorescence technique. The impact of higher-lying states on multiphoton excitation is discussed...

The Potential of Intralesional Rose Bengal to Stimulate T-Cell Mediated Anti-Tumor Responses

Maker, Ajay V; Prabhakar, Bellur; Pardiwala, Krunal
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
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Rose Bengal (RB) is a red synthetic dye that was initially used in the garment industry and has been used safely for decades as a corneal stain by ophthalmologists. Antineoplastic properties of RB have also been observed, though the mechanism of action remained to be elucidated. Recently, interest in RB as a therapeutic cancer treatment has increased due to significant anti-tumor responses with direct tumor injection in human clinical trials for metastatic melanoma. In these patients, there has been the implication that RB may mount a T-cell mediated anti-tumor response and impart antigen-specific responses in distant bystander lesions. This article serves to evaluate the potential of intralesional rose bengal to stimulate T-cell mediated anti-tumor responses in in-vitro, pre-clinical, and clinical studies.

Valor do corante vital Rosa Bengala como auxiliar no diagnóstico de xerose conjuntival em provas de triagem; The value of Rose Bengal as a screening aid to diagnosis of conjunctival xerosis

Wilson, Donald; Roncada, Maria José
Fonte: Universidade de São Paulo. Faculdade de Saúde Pública Publicador: Universidade de São Paulo. Faculdade de Saúde Pública
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/08/1985 POR
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Demonstrou-se o valor do corante vital Rosa Bengala no diagnóstico da xerose conjuntival e determinou-se a prevalência de lesões oculares devidas à hipovitaminose A. Estudou-se uma amostra representativa (501 crianças) da população de 3 a 6 anos do Município de Cotia, SP (Brasil). Realizaram-se exames com e sem o uso de Rosa Bengala nas 501 crianças. As positivas para qualquer das técnicas foram submetidas a prova terapêutica (200.000 UI de vitamina A oral). Examinaram-se as córneas com fluoresceína, quando necessário. Realizou-se dosagem de vitamina A pelo método de Carr-Price. Não se observaram outras lesões, além de xerose conjuntival. Verificou-se que as prevalências de resultados positivos foram de 10,0% sem Rosa Bengala e 18,2% com o corante. As provas terapêuticas revelaram sensibilidade e especificidade baixas para o exame sem Rosa Bengala (18,5% e 14,3%, respectivamente) e altas para o exame com Rosa Bengala (81,5% e 89,0%, respectivamente), o que o indica como método diagnóstico auxiliar para provas de triagem.; The present paper was designed to demonstrate the value of Rose Bengal in the diagnosis of conjunctival xerosis and at the same time determine the prevalence of ocular lesions due to vitamin A deficiency. A representative sample (501 children) between 3 and 6 years of age drawn from the population of the city of Cotia...

Rose Bengal Staining of the Ocular Surface and the Diagnosis of Dry Eye; Coloração da Superfície Ocular por Rosa Bengala e o Diagnóstico do Olho Seco

QUEIROGA, ISABELLA BEZERRA WANDERLEY DE; DINIZ, MARGARETH DE FÁTIMA FORMIGA MELO
Fonte: Universidade Federal da Paraíba Publicador: Universidade Federal da Paraíba
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Formato: application/pdf
Publicado em 31/03/2010 POR
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SUMMARY Since the pioneering study published in 1933 in which Sjögren described rose bengal staining of the exposed interpalpebral zone of the eye as being a characteristic sign of tear deficiency and speculated that the stained cells consisted of dead or devitalized cells, positivity for this staining pattern has been used as a criterion for the diagnosis of dry eye. Material and Methods: Rose bengal solution is instilled into the lower conjunctiva sac. After 15 seconds, the conjunctiva is examined by light passed through a green filter. Rose bengal stain is assumed to demonstrate ocular surface damage by being taken up into dead and degenerate cells. The intensity of staining of the interpalpebral zone is quantified into grades ranging from 1 to 3. Application: The use of rose bengal staining of the ocular surface as a diagnostic criterion for dry eye requires further investigation into the possible staining patterns that may result from use of this dye in view of the different etiologies responsible for this clinical condition. Further investigation is only possible following standardization of the techniques used in the test and implementation of an evaluation system that takes into consideration not only the interpalpebral zone...