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Plasma rico em plaquetas associado a ácido hialurônico e/ ou quitosana para aplicações em medicina regenerativa = : Platelet-rich plasma associated to hyaluronic acid and/ or chitosan for applications in regenerative medicine; Platelet-rich plasma associated to hyaluronic acid and/ or chitosan for applications in regenerative medicine

Andréa Arruda Martins Shimojo
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 27/03/2015 PT
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66.36%
Este trabalho teve como objetivo estudar o desempenho in vitro do plasma rico em plaquetas (PRP) com scaffolds de ácido hialurônico (AH) ou quitosana (CHT) como um scaffold compósito para a proliferação e diferenciação osteogênica de células tronco mesenquimais derivadas de tecido adiposo humano (h-AdMSCs). O PRP é um produto autólogo obtido do sangue total (WB). O ácido hialurônico é um glicosaminoglicano e principal componente do fluido sinovial. A quitosana é um polissacarídeo natural encontrado principalmente na carapaça de crustáceos. O PRP puro (P-PRP, rico em plaquetas e pobre em leucócitos) foi obtido a partir da centrifugação controlada do WB, e ativado com os agonistas soro autólogo e cálcio. Os scaffolds foram estruturados em micropartículas ou esponjas, para atender os requisitos de formulações injetáveis ou sólidas, respectivamente. Nesse contexto, os seguintes scaffolds foram preparados e caracterizados: sólidos porosos (esponjas) de quitosana não estabilizados (PCHTs) e estabilizados (SPCHTs); micropartículas de quitosana-tripolifosfato de sódio (iCHT-TPPs); micropartículas e esponjas de ácido hialurônico autorreticulado (ACPs) e de ácido hialurônico reticulado com 1,4-butanodiol diglicidil éter (HA-BDDE); e esponjas e micropartículas de ACP e CHT (PECs). PCHTs foram preparados por congelamento e liofilização de soluções de CHT...

Dendrimers and derivatives as a potential therapeutic tool in regenerative medicine strategies : a review

Oliveira, Joaquim M.; Salgado, A. J.; Sousa, Nuno; Mano, J. F.; Reis, R. L.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /09/2010 ENG
Relevância na Pesquisa
66.36%
Since the pioneering work dealing with the synthesis and physicochemical aspects of dendrimers, a predictable and tunable set of compositions for therapeutic, scaffolding and imaging systems has been reported. These are well documented, butmanyhot issues should be examined and reviewed. Herein, a review is given on dendritic nanopolymers and their applications that show promise in the field of regenerative medicine. This review begins with a brief overview on research merging nanotechnology and regenerative medicine. Fundamentals of the synthesis and macromolecular structure of dendritic polymers are provided. Dendrimers fulfill the requirements as carriers for gene, nucleic acids, bioactive molecules and peptide/protein delivery aimed at modulate the cells functions, in vitro and in vivo. However, to make use of this potential, toxicological, drug-loading capacity, surface engineering and host–guest chemistries in dendrimers must be addressed and thus are also discussed. We focus on recent work involving dendrimers with applications in tissue engineering and the central nervous system. Due to their innovative character, applications beyond drug delivery systems became possible, namely as scaffolding and chemoattractants for tissue regeneration...

Injectable silk fibroin hydrogels with ionic strength and pH response for tissue engineering and regenerative medicine applications

Yan, Leping; Correia, C.; Pereira, D. R.; Sousa, R. A.; Oliveira, Joaquim M.; Oliveira, A. L.; Reis, R. L.
Fonte: Wiley Publicador: Wiley
Tipo: Conferência ou Objeto de Conferência
Publicado em /09/2013 ENG
Relevância na Pesquisa
66.27%
Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013); The traditional methods to prepare silk fibroin (SF) hydrogels take advantage of the conformation transition from amorphous to β-sheet in aqueous SF solution. During this procedure, the gelation time normally varies from tens of minutes to months depending on the methods used. The relative long gelation time of those methods limits their practicality as in situ injectable systems for incorporation of cells or drugs. Furthermore, most of these approaches are not suitable for cell/drug incorporation in the silk hydrogel. The current study provided an approach to develop SF hydrogel within a few minutes in physiological conditions via peroxidase mediated cross-linking. The influences of the silk concentration,and the content of peroxidase and hydrogen peroxide on the physicochemical properties of the hydrogels were studied. The results showed that the gelation time of the silk hydrogel decreased as increasing silk concentration and peroxidase content, and can be tuned between 4 to 50 minutes. The storage moduli of the hydrogels improved via increasing the hydrogen peroxide content and silk concentration, ranging from 0.25 to 5.20 kPa. The fast formed hydrogels showed extreme elasticity and transparent appearance. There were no differences of the silk hydrogel in the visible light absorbance...

Natural-based nanocomposites for bone tissue engineering and regenerative medicine : a review

Pina, S.; Oliveira, J. M.; Reis, R. L.
Fonte: Wiley Publicador: Wiley
Tipo: Artigo de Revista Científica
Publicado em /01/2015 ENG
Relevância na Pesquisa
66.27%
Tissue engineering and regenerative medicine has been providing exciting technologies for the development of functional substitutes aimed to repair and regenerate damaged tissues and organs. Inspired by the hierarchical nature of bone, nanostructured biomaterials are gaining a singular attention for tissue engineering, owing their ability to promote cell adhesion and proliferation, and hence new bone growth, compared with conventional microsized materials. Of particular interest are nanocomposites involving biopolymeric matrices and bioactive nanosized fi llers. Biodegradability, high mechanical strength, and osteointegration and formation of ligamentous tissue are properties required for such materials. Biopolymers are advantageous due to their similarities with extracellular matrices, specifi c degradation rates, and good biological performance. By its turn, calcium phosphates possess favorable osteoconductivity, resorbability, and biocompatibility. Herein, an overview on the available natural polymer/calcium phosphate nanocomposite materials, their design, and properties is presented. Scaffolds, hydrogels, and fi bers as biomimetic strategies for tissue engineering, and processing methodologies are described. The specifi c biological properties of the nanocomposites...

Regenerative medicine: a review

Porcellini,Adolfo
Fonte: Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular Publicador: Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2009 EN
Relevância na Pesquisa
66.3%
Regenerative medicine is a technique to replace or repair defective or diseased tissue or organs by in vitro design with in vivo usage. It can be considered a relatively new branch of medicine born in 1997 when Whithman DH et al. proposed to integrate platelet enriched plasma (PRP) in fibrin glue. In 1998 Marx et al. demonstrated that PRP was able to induce bone regeneration of the jaw. In the same period it was discovered that a fraction of stem cells of bone marrow origin was able to repair several mesenchymal tissues or organs.

Translational Models for Musculoskeletal Tissue Engineering and Regenerative Medicine

Sah, Robert L.; Ratcliffe, Anthony
Fonte: Mary Ann Liebert, Inc. Publicador: Mary Ann Liebert, Inc.
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
66.51%
The National Institutes of Health–sponsored workshop “Translational Models for Musculoskeletal Tissue Engineering and Regenerative Medicine” was held to describe the utility of various translational models for engineered tissues and regenerative medicine therapies targeting intervertebral disc, cartilage, meniscus, ligament, tendon, muscle, and bone. Participants included leaders in the various topics, as well as National Institutes of Health and Food and Drug Administration. The Food and Drug Administration representatives provided perspectives and needs for studies supported by animal models. Researchers described animal models for specific tissues and addressed the following questions: (1) What are the unmet musculoskeletal clinical needs that may be addressed by tissue engineering and regenerative medicine? (2) Are there appropriate models available? (3) Are there needs to develop standardized animal models? (4) What are the translational pathways that lead to clinical trials and therapeutic development? The workshop provided an effective and succinct summary of the status of various animal models in musculoskeletal regenerative medicine. Although many models are available and serve well to answer a variety of questions, the general consensus was that there is a substantial need for improved and standardized animal models for tissue engineering and regenerative medicine of the musculoskeletal system...

Preface to Special Topic: Biological microfluidics in tissue engineering and regenerative medicine

Jayasinghe, Suwan N.
Fonte: American Institute of Physics Publicador: American Institute of Physics
Tipo: Artigo de Revista Científica
Publicado em 30/03/2011 EN
Relevância na Pesquisa
56.3%
In this special issue of Biomicrofluidics, many manifestations of biological microfluidics have been highlighted that have significance to regenerative biology and medicine. The collated articles demonstrate the applicability of these biological microfluidics for studying a wide range of biomedical problems most useful for understanding and shining light on basic biology to those applications relevant to clinical medicine.

The Pharmacology of Regenerative Medicine

Christ, George J.; Saul, Justin M.; Furth, Mark E.; Andersson, Karl-Erik
Fonte: The American Society for Pharmacology and Experimental Therapeutics Publicador: The American Society for Pharmacology and Experimental Therapeutics
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.5%
Regenerative medicine is a rapidly evolving multidisciplinary, translational research enterprise whose explicit purpose is to advance technologies for the repair and replacement of damaged cells, tissues, and organs. Scientific progress in the field has been steady and expectations for its robust clinical application continue to rise. The major thesis of this review is that the pharmacological sciences will contribute critically to the accelerated translational progress and clinical utility of regenerative medicine technologies. In 2007, we coined the phrase “regenerative pharmacology” to describe the enormous possibilities that could occur at the interface between pharmacology, regenerative medicine, and tissue engineering. The operational definition of regenerative pharmacology is “the application of pharmacological sciences to accelerate, optimize, and characterize (either in vitro or in vivo) the development, maturation, and function of bioengineered and regenerating tissues.” As such, regenerative pharmacology seeks to cure disease through restoration of tissue/organ function. This strategy is distinct from standard pharmacotherapy, which is often limited to the amelioration of symptoms. Our goal here is to get pharmacologists more involved in this field of research by exposing them to the tools...

Science and Ethics: Bridge to the Future for Regenerative Medicine

Patricio, Ventura-Juncá
Fonte: Korean Society for Stem Cell Research Publicador: Korean Society for Stem Cell Research
Tipo: Artigo de Revista Científica
Publicado em /11/2011 EN
Relevância na Pesquisa
46.43%
The objective of this article is to reflect on the relationship between regenerative medicine and ethics, using as references the Aristotelian concept of what is ethical and that of Raessler Van Potter about bioethics. To do this, I will briefly describe the advances in regenerative medicine with stem cells, the strategies for producing pluripotential cells without destroying human embryos, and the great potential of stem cells to improve life for Humanity, noting that for this to be possible, it is necessary to locate the role of regenerative medicine in the context of human values and well being. In this way, this article has a real perspective of the role that regenerative medicine can play in benefitting human beings and engendering respect for human and natural environments.

Concise Review: Cell-Based Strategies in Bone Tissue Engineering and Regenerative Medicine

Ma, Jinling; Both, Sanne K.; Yang, Fang; Cui, Fu-Zhai; Pan, Juli; Meijer, Gert J.; Jansen, John A.; van den Beucken, Jeroen J.J.P.
Fonte: AlphaMed Press Publicador: AlphaMed Press
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
66.27%
Cellular strategies play an important role in bone tissue engineering and regenerative medicine (BTE/RM). Variability in cell culture procedures (e.g., cell types, cell isolation and expansion, cell seeding methods, and preculture conditions before in vivo implantation) may influence experimental outcome. The present review provides an overview of the critical procedures during in vitro and in vivo phases for cell-based strategies (both monoculture and coculture) in BTE/RM.

Rethinking Regenerative Medicine: A Macrophage-Centered Approach

Brown, Bryan N.; Sicari, Brian M.; Badylak, Stephen F.
Fonte: Frontiers Media S.A. Publicador: Frontiers Media S.A.
Tipo: Artigo de Revista Científica
Publicado em 04/11/2014 EN
Relevância na Pesquisa
46.48%
Regenerative medicine, a multi-disciplinary approach that seeks to restore form and function to damaged or diseased tissues and organs, has evolved significantly during the past decade. By adapting and integrating fundamental knowledge from cell biology, polymer science, and engineering, coupled with an increasing understanding of the mechanisms which underlie the pathogenesis of specific diseases, regenerative medicine has the potential for innovative and transformative therapies for heretofore unmet medical needs. However, the translation of novel technologies from the benchtop to animal models and clinical settings is non-trivial and requires an understanding of the mechanisms by which the host will respond to these novel therapeutic approaches. The role of the innate immune system, especially the role of macrophages, in the host response to regenerative medicine based strategies has recently received considerable attention. Macrophage phenotype and function have been suggested as critical and determinant factors in downstream outcomes. The constructive and regulatory, and in fact essential, role of macrophages in positive outcomes represents a significant departure from the classical paradigms of host–biomaterial interactions...

Skeletal stem cells in regenerative medicine

Sonoyama, W.; Coppe, C.; Gronthos, S.; Shi, S.
Fonte: Academic Press Inc Publicador: Academic Press Inc
Tipo: Artigo de Revista Científica
Publicado em //2005 EN
Relevância na Pesquisa
66.27%
Postnatal stem cells have been isolated from a variety of tissues and they are highly expected to have potentiality to be utilized for cell-based clinical therapies. Bone marrow stromal stem cells (BMSSCs) derived from bone marrow stromal tissue have been identified as a population of multipotent mesenchymal stem cells that are capable of differentiating into osteoblasts, adipocytes, chondrocytes, muscle cells, and neural cells. The most significant tissue regeneration trait of BMSSCs is their in vivo bone regeneration capability, which has been widely studied for understanding molecular and cellular mechanisms of osteogenesis, and, more importantly, developing into a stem-cell-based therapy. Recent studies further demonstrated that BMSSC-mediated bone regeneration is a promising approach for regenerative medicine in clinical trials. However, there are some fundamental questions that remain to be answered prior to successful utilization of BMSSCs in clinical therapy. For instance, how to maintain stemness of BMSSCs will be a critical issue for developing methodologies to propagate multi-potential stem cells in vitro, in order to allow the development of effective clinical therapies.; Wataru Sonoyama, Carolyn Coppe, Stan Gronthos, Songtao Shi

Intervertebral disc biology, degeneration and novel tissue engineering and regenerative medicine therapies

Richardson, S.; Mobasheri, A.; Freemont, A.J.; Hoyland, J.A.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
56.21%
Degeneration of the intervertebral disc (IVD) is a major cause of low back pain affecting a large percentage of the population at some point in their lives. Consequently IVD degeneration and its associated low back pain has a huge socio-economic impact and places a burden on health services world-wide. Current treatments remove the symptoms without treating the underlying problem and can result in reoccurrence in the same or adjacent discs. Tissue engineering offers hope that new therapies can be developed which can regenerate the IVD. Combined with this, development of novel biomaterials and an increased understanding of mesenchymal stem cell and IVD cell biology mean that tissue engineering of the IVD may soon become a reality. However for any regenerative medicine approach to be successful there must first be an understanding of the biology of the tissue and the pathophysiology of the disease process. This review covers these key areas and gives an overview of the recent developments in the fields of biomaterials, cell biology and tissue engineering of the IVD.

Marine sponges : a new source of bioactive ceramics for tissue engineering and regenerative medicine applications

Barros, Alexandre A.; Aroso, Ivo; Silva, Tiago H.; Mano, J. F.; Duarte, Ana Rita C.; Reis, R. L.
Fonte: Wiley & Sons, Inc. Publicador: Wiley & Sons, Inc.
Tipo: Conferência ou Objeto de Conferência
Publicado em /10/2013 ENG
Relevância na Pesquisa
66.27%
Publicado em : "Journal of Tissue Engineering and Regenerative Medicine", vol, 7, supp. 1 (2013); Marine organisms are exceptionally rich in natural products and present huge prospective for biomedical applications. In this work we have studied the potential of bioceramics from different sponge species, namely Petrosia ficidormis, Agelas oroides and Chondrosia reniformis, for novel biomedical applications. Studies reported in the literature have already demonstrated the potential of carbonate corals as a source of bioceramics. However, similar studies directed towards the valorization of marine sponge skeletons are still missing. The bioceramics, exempt of organic components, were obtained after calcination of the sponges at 750°C for 6 hours in a furnace. The powder was recovered and Scanning electron microscopy (SEM) was used to observe the morphology and gain insight of the elements spatial arrangement. Spectroscopic elemental analysis (EDS) was used to determine the chemical composition and has shown that Petrosia ficidormis skeleton is constituted mainly by silicate, while Chondrosia reniformis spicules are mostly calcium carbonates. On the other hand, the ceramics obtained from Agelas oroides present a combination of silicate and calcium salts. In vitro bioactivity of the bioceramics was evaluated in simulated body fluid (SBF)...

Phosphorous-containing polymers for regenerative medicine

Watson, Brendan M.; Kasper, F. Kurtis; Mikos, Antonios G.
Fonte: Universidade Rice Publicador: Universidade Rice
Tipo: Journal article; Text; post-print
ENG
Relevância na Pesquisa
66.27%
Disease and injury have resulted in a large, unmet need for functional tissue replacements. Polymeric scaffolds can be used to deliver cells and bioactive signals to address this need for regenerating damaged tissue. Phosphorous-containing polymers have been implemented to improve and accelerate the formation of native tissue both by mimicking the native role of phosphorous groups in the body and by attachment of other bioactive molecules. This manuscript reviews the synthesis, properties, and performance of phosphorous-containing polymers that can be useful in regenerative medicine applications.

Magnetic force-based tissue engineering and regenerative medicine

Castro, E.; Mano, J. F.
Fonte: American Scientific Publishers Publicador: American Scientific Publishers
Tipo: Artigo de Revista Científica
Publicado em /07/2013 ENG
Relevância na Pesquisa
66.27%
Among other biomedical applications, magnetic nanoparticles and liposomes have a vast field of applications in tissue engineering and regenerative medicine. Magnetic nanoparticles and liposomes, when introduced into cells to be cultured, maneuver the cell's positioning by the appropriate use of magnets to create more complex tissue structures than those that are achieved by conventional culture methods.

Preclinical Models for Translating Regenerative Medicine Therapies for Rotator Cuff Repair

Derwin, Kathleen A.; Baker, Andrew Ryan; Iannotti, Joseph P.; McCarron, Jesse A.
Fonte: Mary Ann Liebert, Inc. Publicador: Mary Ann Liebert, Inc.
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
66.4%
Despite improvements in the understanding of rotator cuff pathology and advances in surgical treatment options, repairs of chronic rotator cuff tears often re-tear or fail to heal after surgery. Hence, there is a critical need for new regenerative repair strategies that provide effective mechanical reinforcement of rotator cuff repair as well as stimulate and enhance the patient's intrinsic healing potential. This article will discuss and identify appropriate models for translating regenerative medicine therapies for rotator cuff repair. Animal models are an essential part of the research and development pathway; however, no one animal model reproduces all of the features of the human injury condition. The rat shoulder is considered the most appropriate model to investigate the initial safety, mechanism, and efficacy of biologic treatments aimed to enhance tendon-to-bone repair. Whereas large animal models are considered more appropriate to investigate the surgical methods, safety and efficacy of the mechanical—or combination biologic/mechanical—strategies are ultimately needed for treating human patients. The human cadaver shoulder model, performed using standard-of-care repair techniques, is considered the best for establishing the surgical techniques and mechanical efficacy of various repair strategies at time zero. While preclinical models provide a critical aspect of the translational pathway for engineered tissues...

Regenerative medicine: Current therapies and future directions

Mao, Angelo S.; Mooney, David J.
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
56.48%
Organ and tissue loss through disease and injury motivate the development of therapies that can regenerate tissues and decrease reliance on transplantations. Regenerative medicine, an interdisciplinary field that applies engineering and life science principles to promote regeneration, can potentially restore diseased and injured tissues and whole organs. Since the inception of the field several decades ago, a number of regenerative medicine therapies, including those designed for wound healing and orthopedics applications, have received Food and Drug Administration (FDA) approval and are now commercially available. These therapies and other regenerative medicine approaches currently being studied in preclinical and clinical settings will be covered in this review. Specifically, developments in fabricating sophisticated grafts and tissue mimics and technologies for integrating grafts with host vasculature will be discussed. Enhancing the intrinsic regenerative capacity of the host by altering its environment, whether with cell injections or immune modulation, will be addressed, as well as methods for exploiting recently developed cell sources. Finally, we propose directions for current and future regenerative medicine therapies.

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Santos, Lívia; Reis, R. L.; Gomes, Manuela E.
Fonte: Cell Publicador: Cell
Tipo: Artigo de Revista Científica
Publicado em /08/2015 ENG
Relevância na Pesquisa
66.27%
Mechanical stimulus is of upmost importance in tissues developmental and regeneration processes as well as in maintaining body homeostasis. Classical physiological reactions encompass an increase of blood vessel diameter upon exposure to high blood pressure, or the expansion of cortical bone after continuous high-impact exercise. At a cellular level, it is well established that extracellular stiffness, topography, and remote magnetic actuation are instructive mechanical signals for stem cell differentiation. Based on this, biomaterials and their properties can be designed to act as true stem cell regulators, eventually leading to important advances in conventional tissue engineering techniques. This review identifies the latest advances and tremendous potential of magnetic actuation within the scope of regenerative medicine and tissue engineering. ; European project Polaris, FP7 REGPOT

Stromal vascular fraction cell sheets angiogenic potential for tissue engineering and regenerative medicine applications

Costa, M.; Cerqueira, M. T.; Rodrigues, D. B.; Santos, T. C.; Marques, A. P.; Pirraco, Rogério P.; Reis, R. L.
Fonte: Mary Ann Liebert Publicador: Mary Ann Liebert
Tipo: Conferência ou Objeto de Conferência
Publicado em /08/2015 ENG
Relevância na Pesquisa
66.27%
One of the biggest concerns in the Tissue Engineering field is the correct vascularization of engineered constructs. Strategies involving the use of endothelial cells are promising but adequate cell sourcing and neo-vessels stability are enduring challenges. In this work, we propose the hypoxic pre-conditioning of the stromal vascular fraction (SVF) of human adipose tissue to obtain highly angiogenic cell sheets (CS). For that, SVF was isolated after enzymatic dissociation of adipose tissue and cultured until CS formation in normoxic (pO2=21%) and hypoxic (pO2=5%) conditions for 5 and 8 days, in basal medium. Immunocytochemistry against CD31 and CD146 revealed the presence of highly branched capillary-like structures, which were far more complex for hypoxia. ELISA quantification showed increased VEGF and TIMP-1 secretion in hypoxia for 8 days of culture. In a Matrigel assay, the formation of capillary-like structures by endothelial cells was more prominent when cultured in conditioned medium recovered from the cultures in hypoxia. The same conditioned medium increased the migration of adipose stromal cells in a scratch assay, when compared with the medium from normoxia. Histological analysis after implantation of 8 days normoxic- and hypoxic-conditioned SVF CS in a hindlimb ischemia murine model showed improved formation of neo-blood vessels. Furthermore...