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Iron chelating-mediated antioxidant activity of Plectranthus barbatus extract on mitochondria

MAIOLI, Marcos A.; ALVES, Larissa C.; CAMPANINI, Andre L.; LIMA, Michele C.; DORTA, Daniel J.; GROPPO, Milton; CAVALHEIRO, Alberto J.; CURTI, Carlos; MINGATTO, Fabio E.
Fonte: ELSEVIER SCI LTD Publicador: ELSEVIER SCI LTD
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
475.10996%
Plectranthus barbatus Andrews (Lamiaceae) is a popular medicinal plant used to treat gastrointestinal and hepatic ailments. In this work, we assessed the antioxidant activity of the aqueous extract of P. barbatus leaves on Fe(2+)-citrate-mediated membrane lipid peroxidation in isolated rat liver mitochondria, as well in non-mitochondrial systems: DPPH reduction, (center dot)OH scavenging activity, and iron chelation by prevention of formation of the Fe(2+)-bathophenanthroline disulfonic acid (BPS) complex. Within all the tested concentrations (15-75 mu g/ml), P. barbatus extract presented significant free radical-scavenging activity (IC(50) = 35.8 +/- 0.27 mu g/ml in the DPPH: assay and IC(50) = 69.1 +/- 0.73 mu g/ml in the (center dot)OH assay) and chelated iron (IC(50) = 30.4 +/- 3.31 mu g/ml). Over the same concentration range, the plant extract protected mitochondria against Fe(2+)/citrate-mediated swelling and malondialdehyde production, a property that persisted even after simulation of its passage through the digestive tract. These effects could be attributed to the phenolic compounds, nepetoidin - caffeic acid esters, present in the extract. Therefore, P. barbatus extract prevents mitochondrial membrane lipid peroxidation, probably by chelation of iron...

Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Rossi, G. D.; Santos, C. D.; Carvalho, G. A.; Alves, D. S.; Pereira, L. L. S.; Carvalho, G. A.
Fonte: ENTOMOLOGICAL SOC BRASIL; LONDRINA, Publicador: ENTOMOLOGICAL SOC BRASIL; LONDRINA,
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
477.01023%
Plant extracts represent a great source of molecules, with insecticidal activity, which are used for pest control in several crop production systems. This work aimed to evaluate the toxicity of an aqueous extract of leaves of castor bean against larvae of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) in search for different classes of molecules with insecticidal activities by using in vitro assays. The effects of the castor bean leaf extract on the food utilization, development, and survival of S. frugiperda larvae was evaluated by feeding the larvae an artificial diet supplemented with different concentrations of the extract (0%, 1%, 2.5%, 5%, and 10% w/v). The effects observed were dose-dependent, and the highest concentration evaluated (10% w/v) was the one the most affected food utilization by altering the nutritional indices, as well as larval weight gain, development time, and survivorship. In vitro assays to detect saponins, lectins, and trypsin inhibitors in the castor bean leaf extract were performed, but only trypsin inhibitors were detected. No preference for the diet source was detected in S. frugiperda by feeding the larvae in choice experiments with diets containing different concentrations of the castor bean extract tested. The data obtained indicate the existence of a potential molecule in the tested extract of castor bean to be used as an alternative insecticide to be integrated in the management of S. frugiperda.

Uso de extratos vegetais como promotores do crescimento em frangos de corte; Use of plant extracts as growth promoters for broiler chickens

Barreto, Marina Sígolo Rodrigues
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 02/07/2007 PT
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Nas últimas décadas, a produção de carne de frango vem se intensificando por expressivos avanços tecnológicos. Nutricionalmente, os promotores de crescimento antimicrobianos (antibióticos e quimioterápicos) foram essenciais, beneficiando o desempenho e a eficiência alimentar, quando utilizados como aditivos nas dietas, em doses subterapêuticas. Apesar da comprovada contribuição no desempenho das aves, os antibióticos promotores de crescimento passaram a ser vistos como fatores de risco para a saúde humana, devido ao potencial desenvolvimento da resistência bacteriana cruzada em humanos. Recentemente, têm sido desenvolvidas diversas alternativas aos antibióticos promotores de crescimento, incluindo probióticos, prebióticos, ácidos orgânicos, enzimas e extratos vegetais. Seguindo essa tendência, este estudo teve o objetivo de avaliar a eficácia do uso de extratos vegetais como alternativas aos antimicrobianos promotores de crescimento em dietas de frangos de corte. Foram realizados dois experimentos com frangos de corte para avaliar os efeitos de diferentes extratos vegetais no desempenho, na energia metabolizável da dieta e na morfometria dos órgãos. O experimento de desempenho envolveu 1200 frangos de corte machos criados em galpão experimental no período de 1 a 42 dias de idade...

Avaliação do potencial fotoquimioprotetor do extrato de Protium heptaphyllum da Amazônia em gel de aplicação tópica; Evaluation of photochemoprotective potencial of Protium heptaphyllum Amazon plant extract added in gel formulation for topical application

Forte, Ana Luíza Scarano Aguillera
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 28/02/2012 PT
Relevância na Pesquisa
480.22812%
A pele atua como barreira entre o meio externo e o organismo. Mesmo possuindo um grande número de mecanismos de defesa antioxidante, certas situações como a exposição prolongada à radiação ultravioleta (RUV), são capazes de romper o equilíbrio pró-oxidante/antioxidante no organismo, provocando um grande aumento na concentração de espécies reativas de oxigênio (EROs). A administração tópica de antioxidantes é um eficiente modo para enriquecer o sistema protetor cutâneo endógeno, e assim uma estratégia de sucesso para reduzir os danos oxidativos causados à pele pela RUV. O objetivo do presente trabalho foi avaliar os extratos vegetais de Eugenia biflora, Eugenia protenta, Protium heptaphyllum, e Miconia minutiflora quanto as suas propriedades antioxidantes, bem como sua citotoxicidade, e verificar a eficácia do extrato de Protium heptaphyllum como agente fotoquimioprotetor in vivo. A atividade antioxidante dos extratos foi avaliada por diferentes métodos in vitro que simulam a formação de EROs e os valores foram expressos como a concentração de extrato necessária para promover 50% da ação antioxidante (IC50). Todos os extratos estudados tiveram valores de IC50 baixo, o que representa um grande potencial antioxidante. Além disso...

Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

Della Torre, A.; Albuquerque, L. B. L.; Farrapo, N. M.; Oshima-Franco, Y.; Santos, M. G.; Tavares, R. V. S.; Rodas, A. C. D.; Dal Belo, C. A.; Cardoso, C. R. P.; Varanda, Eliana Aparecida; Groppo, F. C.; Lopes, P. S.
Fonte: Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) Publicador: Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
Tipo: Artigo de Revista Científica Formato: 190-198
ENG
Relevância na Pesquisa
475.10996%
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 mu g/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.

Estudo do extrato de Bauhinia forficata L. sobre o diabete em ratas prenhes

Volpato, Gustavo Tadeu; Damasceno, Débora Cristina; Calderon, Iracema de Mattos Paranhos; Rudge, Marilza Vieira Cunha
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 49-55
POR
Relevância na Pesquisa
479.42594%
The objective of this paper was to study the aqueous extract of Bauhinia forficata L. (pata-de-vaca) effects on streptozotocin-induced diabete pregnant rats. We used Wistar pregnant rats, dividided in 3 experimental groups: control (C, n=7), non-treated diabetics (DNT, n=7) and streptozotocin induced-diabetics treated with an aqueous extract of pata-de-vaca leaves, 200 mg/kg dose (DT, n=7). The animals received the extract through a gastric tube (gavage). The blood glucose level were verified on day 0, 5, 14 and 20 of pregnancy. During pregnancy, the daily mean water intake, food intake and average maternal weight gains of rats were measured. The results demonstrated that plant extract reduced the postimplantation loss porcentage, increasing the number of live fetuses likely to the control group. We found increased food and water intake of the DT and DNT pregnant rats compared to control due to hyperglycemic state. We also observed average maternal weight gains was likely to the DT and control groups on different pregnant periods, suggesting treatment with the plant contributed for the rat weight gains. The blood glucose level of dams did not present significative differences between DT and DNT groups. Thus, the B. forficata aqueous extract...

Evaluation of Strychnos pseudoquina St. Hil. leaves extract on gastrointestinal activity in mice

Aparecido Da Silva, Marcelo; Murino Rafacho, Bruna Paola; Hiruma-Lima, Clelia Akiko; Machado Da Rocha, Lúcia Regina; Campaner Dos Santos, Lourdes; Sannomiya, Miriam; Monteiro Souza-Brito, Alba Regina; Vilegas, Wagner
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 881-885
ENG
Relevância na Pesquisa
475.80016%
Strychnos pseudoquina St. Hil. (Loganiaceae) was investigated for its ability to protect the gastric mucosa against injuries caused by non-steroidal anti-inflammatory drugs (piroxicam) and a necrotizing agent (HCl/EtOH) in mice. The MeOH extract and enriched alkaloidic fraction (EAF) provided significant protection in experimental models wheer used at doses of 250 and 1000 mg/kg. In vivo tests were carried out to evaluate for possible toxic effects and no mortality was observed up to the 5 g/kg dose level. Phytochemical investigation led to the isolation of a new indole alkaloid, which elucidated the observed pharmacological effects. © 2005 Pharmaceutical Society of Japan.

Assessment of DNA damage by extracts and fractions of Strychnos pseudoquina, a Brazilian medicinal plant with antiulcerogenic activity

Santos, F. V.; Colus, I. M S; Silva, M. A.; Vilegas, Wagner; Varanda, E. A.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 1585-1589
ENG
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Strychnos pseudoquina St. Hil. is a native plant of the Brazilian Savannah, used in popular medicine to treat a number of conditions. Since it contains large quantities of alkaloids with proven antiulcer activity, we tested the genotoxic potential of crude extracts and fractions containing alkaloids and flavonoids from the leaves of this plant, on Salmonella typhimurium and performed the micronucleus test on peripheral blood cells of mice treated in vivo. The results showed that the methanol extract of the leaves of S. pseudoquina is mutagenic to the TA98 (-S9) and TA100 (+S9, -S9) strains of Salmonella. The dichloromethane extract was not mutagenic to any of the tested strains. Fractions enriched with alkaloids or flavonoids were not mutagenic. In vivo tests were done on the crude methanol extract in albino Swiss mice, which were treated, by gavage, with three different doses of the extract. The highest dose tested (1800 mg/kg b.w.) induced micronuclei after acute treatment, confirming the mutagenic potential of the methanol extract of the leaves of S. pseudoquina. In high doses, constituents of S. pseudoquina compounds act on DNA, causing breaks and giving rise to micronuclei in the blood cells of treated animals. © 2006 Elsevier Ltd. All rights reserved.

The in vitro antimicrobial activity of natural infant fluoride-free toothpastes on oral micro-organisms

Carvalho, Fabíola G.; Negrini, Thais de Cássia; Sacramento, Luis Victor S.; Hebling, Josimeri; Spolidorio, Denise M. P.; Duque, Cristiane
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 3-8
ENG
Relevância na Pesquisa
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Purpose: The objective of this study was to evaluate the antimicrobial activity of six toothpastes for infants: 3 fluoride-free experimental toothpastes - cashew-based, mango-based and without plant extract and fluoride compared with 2 commercially fluoride-free toothpastes and 1 fluoridated toothpastes. Methods: Six toothpastes for infants were evaluated in this study: (1) experimental cashew-based toothpaste; (2) experimental mango-based toothpaste; (3) experimental toothpaste without plant extract and fluoride (negative control); (4) First Teeth brand toothpaste; (5) Weleda brand toothpaste; and (6) Tandy brand toothpaste (positive control). The antimicrobial activity was recorded against Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, and Candida albicans using the agar plate diffusion test. Results: First Teeth, Weleda, mango-based toothpaste, and toothpaste without plant extract presented no antimicrobial effect against any of the tested micro-organisms. Cashew toothpaste had antimicrobial activity against S mutans, S sobrinus, and L acidophilus, but it showed no antimicrobial activity against C albicans. There was no statistical difference between the inhibition halo of cashew and Tandy toothpastes against S mutans and L acidophilus. Conclusions: Cashew fluoride-free toothpaste had inhibitory activity against Streptococcus mutans and Lactobacillus acidophilus...

Genotoxicity of the medicinal plant Maytenus robusta in mammalian cells in vivo.

Raymundo, T. M.; Favilla, M.; Niero, R.; Andrade, S. F.; Maistro, E. L.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 2847-2854
ENG
Relevância na Pesquisa
474.1637%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 08/51175-7; Plants belonging to the Celastraceae family have been used in traditional medicine for their analgesic, anti-inflammatory and anti-ulcerogenic properties, among others. Maytenus ilicifolia is the principal species of this family, and is used in the treatment of gastric ulcers. However, owing to its inadequate management in Brazil, the species is becoming extinct and is being substituted with Maytenus robusta, which also displays gastroprotective activity. The aim of this study was to evaluate the genotoxic effects of M. robusta hydroalcoholic extract in vivo, using the comet assay and micronucleus test. Three doses (50, 250 and 500 mg/kg body weight) were administered to mice orally 2 times at 24-h intervals. Cytotoxicity was assessed by scoring 200 consecutive total polychromatic and normochromatic erythrocytes to calculate their ratio. Parametric (analysis of variance/Tukey) and non-parametric (Kruskal-Wallis/Dunn post hoc) tests were used to evaluate the results according to the nature of the data distribution. The results showed a significant increase in the frequency of DNA damage on leukocytes at the 2 higher doses tested, but the extract did not enhance micronucleus frequency in bone marrow cells. Our findings showed that after 48 h of treatment...

Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity

Katiki, Luciana M.; Ferreira, Jorge F.S.; Gonzalez, Javier M.; Zajac, Anne M.; Lindsay, David S.; Chagas, Ana Carolina S.; Amarante, Alessandro F.T.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 218-227
ENG
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Although tannin-rich forages are known to increase protein uptake and to reduce gastrointestinal nematode infections in grazing ruminants, most published research involves forages with condensed tannins (CT), while published literature lacks information on the anthelmintic capacity, nutritional benefits, and antioxidant capacity of alternative forages containing hydrolyzable tannins (HT). We evaluated the anthelmintic activity and the antioxidant capacity of plant extracts containing either mostly CT, mostly HT, or both CT and HT. Extracts were prepared with 70% acetone, lyophilized, redissolved to doses ranging from 1.0mg/mL to 25mg/mL, and tested against adult Caenorhabditis elegans as a test model. The extract concentrations that killed 50% (LC50) or 90% (LC90) of the nematodes in 24h were determined and compared to the veterinary anthelmintic levamisole (8mg/mL). Extracts were quantified for CT by the acid butanol assay, for HT (based on gallic acid and ellagic acid) by high-performance liquid chromatography (HPLC) and total phenolics, and for their antioxidant activity by the oxygen radical absorbance capacity (ORAC) assay. Extracts with mostly CT were Lespedeza cuneata, Salix X sepulcralis, and Robinia pseudoacacia. Extracts rich in HT were Acer rubrum...

Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae)

Lopes, Andrey P.; Bagatela, Bianca S.; Rosa, Paulo C. P.; Nanayakkara, Dhammika N. P.; Carlos Tavares Carvalho, José; Maistro, Edson Luis; Bastos, Jairo K.; Perazzo, Fábio F.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
478.59168%
The crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. The crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 μg/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 μg/mL), methylene chloride (IC 50 = 5.9 g/mL), ethyl acetate (IC50 = 8.0 g/mL), 4-nerolidylcatechol (IC50 = 8.6 g/mL), and the sterol fractions (IC50 > 12.5 μg/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 μg/mL. 4-Nerolidylcatechol (IC50 = 0.4 μg/mL) and methylene chloride fraction (IC50 = 2.3 μg/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol...

Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry

de Oliveira, Jonatas Rafael; de Castro, Vinicius Carlos; Vilela, Polyana das Graças Figueiredo; Camargo, Samira Esteves Afonso; Carvalho, Cláudio Antonio Talge; Jorge, Antonio Olavo Cardoso; de Oliveira, Luciane Dias
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
484.5079%
Background: With the emergence of strains resistant to conventional antibiotics, it is important to carry studies using alternative methods to control these microorganisms causing important infections, such as the use of products of plant origin that has demonstrated effective antimicrobial activity besides biocompatibility. Therefore, this study aimed to evaluate the antimicrobial activity of plant extracts of Equisetum arvense L., Glycyrrhiza glabra L., Punica granatum L. and Stryphnodendron barbatimam Mart. against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Candida albicans, Candida tropicalis, and Candida glabrata, and to analyze the cytotoxicity of these extracts in cultured murine macrophages (RAW 264.7).Methods: Antimicrobial activity of plant extracts was evaluated by microdilution method based on Clinical and Laboratory Standards Institute (CLSI), M7-A6 and M27-A2 standards. The cytotoxicity of concentrations that eliminated the microorganisms was evaluated by MTT colorimetric method and by quantification of proinflammatory cytokines (IL-1β and TNF-α) using ELISA.Results: In determining the minimum microbicidal concentration, E. arvense L., P. granatum L., and S. barbatimam Mart. extracts at a concentration of 50 mg/mL and G. glabra L. extract at a concentration of 100 mg/mL...

A laboratory method to estimate the efficiency of plant extract to neutralize soil acidity

Cassiolato,Marcelo E.; Miyazawa,Mário; Meda,Anderson R.; Pavan,Marcos A.
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2002 EN
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578.17125%
Water-soluble plant organic compounds have been proposed to be efficient in alleviating soil acidity. Laboratory methods were evaluated to estimate the efficiency of plant extracts to neutralize soil acidity. Plant samples were dried at 65ºC for 48 h and ground to pass 1 mm sieve. Plant extraction procedure was: transfer 3.0 g of plant sample to a becker, add 150 ml of deionized water, shake for 8 h at 175 rpm and filter. Three laboratory methods were evaluated: sigma (Ca+Mg+K) of the plant extracts; electrical conductivity of the plant extracts and titration of plant extracts with NaOH solution between pH 3 to 7. These methods were compared with the effect of the plant extracts on acid soil chemistry. All laboratory methods were related with soil reaction. Increasing sigma (Ca+Mg+K), electrical conductivity and the volume of NaOH solution spent to neutralize H+ ion of the plant extracts were correlated with the effect of plant extract on increasing soil pH and exchangeable Ca and decreasing exchangeable Al. It is proposed the electrical conductivity method for estimating the efficiency of plant extract to neutralize soil acidity because it is easily adapted for routine analysis and uses simple instrumentations and materials.

Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

Della Torre,A; Albuquerque,LBL; Farrapo,NM; Oshima-Franco,Y; Santos,MG; Tavares,RVS; Rodas,ACD; Dal Belo,CA; Cardoso,CRP; Varanda,EA; Groppo,FC; Lopes,PS
Fonte: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP Publicador: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2011 EN
Relevância na Pesquisa
475.10996%
Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.

Antibacterial properties of silver nanoparticles synthesized using Pulicaria glutinosa plant extract as a green bioreductant

Khan, Mujeeb; Khan, Shams Tabrez; Khan, Merajuddin; Adil, Syed Farooq; Musarrat, Javed; Al-Khedhairy, Abdulaziz A; Al-Warthan, Abdulrahman; Siddiqui, Mohammed Rafiq H; Alkhathlan, Hamad Z
Fonte: Dove Medical Press Publicador: Dove Medical Press
Tipo: Artigo de Revista Científica
Publicado em 28/07/2014 EN
Relevância na Pesquisa
481.41%
The antibacterial properties of nanoparticles (NPs) can be significantly enhanced by increasing the wettability or solubility of NPs in aqueous medium. In this study, we investigated the effects of the stabilizing agent on the solubility of silver NPs and its subsequent effect on their antimicrobial activities. Silver NPs were prepared using an aqueous solution of Pulicaria glutinosa plant extract as bioreductant. The solution also acts as a capping ligand. During this study, the antimicrobial activities of silver NPs, as well as the plant extract alone, were tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Micrococcus luteus. Silver NPs were prepared with various concentrations of the plant extract to study its effect on antimicrobial activity. Interestingly, various concentrations of P. glutinosa extract did not show any effect on the growth of tested bacteria; however, a significant effect on the antimicrobial property of plant extract capped silver NPs (Ag-NPs-PE) was observed. For instance, the half maximal inhibitory concentration values were found to decrease (from 4% to 21%) with the increasing concentrations of plant extract used for the synthesis of Ag-NPs-PE. These results clearly indicate that the addition of P. glutinosa extracts enhances the solubility of Ag-NPs-PE and...

Plant extract enhances the viability of Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus acidophilus in probiotic nonfat yogurt

Michael, Minto; Phebus, Randall K; Schmidt, Karen A
Fonte: BlackWell Publishing Ltd Publicador: BlackWell Publishing Ltd
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
474.1637%
A commercial plant extract (prepared from olive, garlic, onion and citrus extracts with sodium acetate (SA) as a carrier) was evaluated to extend the viability of yogurt starter and probiotic bacteria as a means to enhance the shelf life of live and active culture, probiotic nonfat yogurt. Yogurts prepared from three different formulas (0.5* plant extract, 0.25* SA, or no supplement) and cultures (yogurt starter plus Bifidobacterium animalis,Lactobacillus acidophilus, or both probiotics) were assessed weekly during 29 days of storage at 5°C. Supplemented yogurt mixes had greater buffering capacities than non-supplemented yogurt mixes. At the end of storage, Lactobacillus bulgaricus and L. acidophilus counts in supplemented yogurts were greater compared with non-supplemented yogurts. Supplementation did not affect Streptococcus thermophilus and B. animalis counts. Hence the greater buffering capacity of yogurt containing plant extract could enhance the longevity of the probiotics, L. bulgaricus and L. acidophilus, during storage.

Estudo da ação antiofídica e da toxicidade aguda do extrato aquoso de Schizolobium parahyba; Snake venom; Inhibition; Plant extract; Vegetal extract; Acute toxicity

Mendes, Mirian Machado
Fonte: Universidade Federal de Uberlândia Publicador: Universidade Federal de Uberlândia
Tipo: Dissertação
POR
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CAPÍTULO II - Muitas plantas medicinais são recomendadas para o tratamento de picadas de serpentes. O extrato aquoso preparado com as folhas de Schizolobium parahyba foi ensaiado para verificar sua habilidade em inibir algumas atividades enzimáticas e biol ógicas induzidas por peçonhas de Bothrops pauloensis e Crotalus durissus terriffcus, bem como suas toxinas isoladas, neuwiedase (metaloprotease), BnSP7 (PLA2 Lys-49 básica) e CB (PLA2 do complexo crotoxina). As atividades fosfolipase A2, coagulante, fibrinogenolítica, hemorrágica e miotóxica induzidas por B. pauloensis e C. d. terrificus, bem como suas toxinas isoladas foram significativamente inibidas quando diferentes quantidades de S. parahyba foram incubadas previamente com as peçonhas e toxinas antes dos ensaios. No entanto, quando S. parahyba foi administrada na mesma via, após a injeção de peçonha ou das toxinas isoladas, os danos teciduais locais como hemorragia e miotoxicidade foram somente parcialmente inibidos. Avaliamos também o efeito inibitório de S. parahyba sobre o espalhamento das proteínas da peçonha da área da injeção para a circulação sistêmica. A neutralização das alterações sistêmicas induzidas pela injeção i.m. da peçonha de B. pauloensis foi avaliada mensurando o número de plaquetas e pelos níveis de fibrinogênio plasmático que foram significativamente mantidos quando a inoculação do extrato de S. parahyba ocorreu na mesma via...

Anti-arthritic potential of the plant Justicia gendarussa Burm F

Paval,Jaijesh; Kaitheri,Srinivasan Keloth; Potu,Bhagath Kumar; Govindan,Sreejith; Kumar,Raju Suresh; Narayanan,Sareesh Naduvil; Moorkoth,Sudheer
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2009 EN
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476.11582%
OBJECTIVE: To evaluate the anti-arthritic potential of the plant Justicia gendarussa using two different rat models. MATERIALS AND METHOD: The anti-arthritic potential of the alcoholic extract of the plant Justicia gendarussa was evaluated using the Freund's adjuvant-induced and collagen-induced arthritic rat models. The rats were treated with the ethanolic extract of Justicia gendarussa and with standard aspirin. RESULTS: The ethanolic extract of Justicia gendarussa showed significant anti-arthritic activity that was statistically similar to that of aspirin. Our results suggest that the alcoholic extract of Justicia gendarussa exhibits significant anti-arthritic potential.

Anti-arthritic potential of the plant Justicia gendarussa Burm F

Paval, Jaijesh; Kaitheri, Srinivasan Keloth; Potu, Bhagath Kumar; Govindan, Sreejith; Kumar, Raju Suresh; Narayanan, Sareesh Naduvil; Moorkoth, Sudheer
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/04/2009 ENG
Relevância na Pesquisa
476.11582%
OBJECTIVE: To evaluate the anti-arthritic potential of the plant Justicia gendarussa using two different rat models. MATERIALS AND METHOD: The anti-arthritic potential of the alcoholic extract of the plant Justicia gendarussa was evaluated using the Freund's adjuvant-induced and collagen-induced arthritic rat models. The rats were treated with the ethanolic extract of Justicia gendarussa and with standard aspirin. RESULTS: The ethanolic extract of Justicia gendarussa showed significant anti-arthritic activity that was statistically similar to that of aspirin. Our results suggest that the alcoholic extract of Justicia gendarussa exhibits significant anti-arthritic potential.