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In vitro antimicrobial activity of Caesalpinia ferrea Martius fruits against oral pathogens

SAMPAIO, Fabio C.; PEREIRA, Maria do Socorro V.; DIAS, Celidarque S.; COSTA, Vicente Carlos O.; CONDE, Nikeila C. O.; BUZALAF, Marilia A. R.
Fonte: ELSEVIER IRELAND LTD Publicador: ELSEVIER IRELAND LTD
Tipo: Artigo de Revista Científica
ENG
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Aim: In the Amazon region of Brazil, the fruits of Caesalpinia ferrea Martius (Brazilian ironwood) are widely used as an antimicrobial and healing medicine in many situations including oral infections. This study aimed to evaluate the antimicrobial activity of Caesalpinia ferrea Martius fruit extract against oral pathogens. Materials and methods: Polyphenols estimation and spectral analysis ((1)H NMR) of the methanol extract were carried out. The microorganisms Candida albicans, Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis and Lactobacillus casei were tested using the microdilution method for planktonic cells (MIC) and a multispecies biofilm model. Chlorhexidine was used as positive control. Results: Polyphenols in the extract were estimated at 7.3% and (1)H NMR analysis revealed hydroxy phenols and methoxilated compounds. MIC values for Candida albicans, Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis and Lactobacillus casei were 25.0, 40.0, 66.0, 100.0, 66.0 mu g/mL, respectively. For the biofilm assay, chlorhexidine and plant extract showed no growth at 10(-4) and 10(-5) microbial dilution, respectively. At 10-4 and 10-5 the growth values (mean +/- SD) of the negative controls (DMSO and saline solution) for Streptococcus mutans...

Antimicrobial activity and enterococcus faecalis biofilm formation on chlorhexidine varnishes

Arias-Moliz, Maria-Teresa; Ferrer-Luque, Carmen-Maria; Gonzalez-Rodriguez, Maria-Paloma; Navarro-Escobar, Esther; de Freitas, Marcia-Furtado-Antunes; Baca, Pilar
Fonte: MEDICINA ORAL S L; VALENCIA Publicador: MEDICINA ORAL S L; VALENCIA
Tipo: Artigo de Revista Científica
ENG
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Objective: To evaluate, in vitro, the antimicrobial activity and biofilm formation of three chlorhexidine varnishes in four Enterococcus faecalis strains: E. faecalis ATCC 29212, E. faecalis EF-D1 (from failed endodontic treatment), E. faecalis 072 (cheese) and E. faecalis U-1765 (nosocomial infection), and one Enterococcus durans strain (failed endodontic treatment). Study Design: The direct contact test was used to study the antimicrobial activity. Bacterial suspensions were exposed for one hour to EC40, Cervitec (CE) and Cervitec Plus (CEP) varnishes. "Eradication" was defined as 100% bacterial kill. The formation of enterococci biofilms was tested on the surface of the varnishes after 24 hours of incubation and expressed as percentage of biofilm reduction. Results: EC40 eradicated all strains except E. faecalis ATCC 29212, where 98.78% kill was achieved. CE and CEP showed antimicrobial activity against all the strains, but most clearly against E. durans and E. faecalis 072. EC40 completely inhibited the formation of biofilm of E. faecalis ATCC 29212, E. faecalis 072 and E. durans. CE and CEP led to over 92% of biofilm reduction, except in the case of E. faecalis U-1765 on CEP (76.42%). Conclusion: The three varnishes studied were seen to be effective in killing the tested strains of enterococci and in inhibiting the formation of biofilm...

In vitro enterococcus faecalis biofilm formation on five adhesive systems

Baca, Pilar; de Freitas, Marcia Furtado-Antunes; Ferrer-Luque, Carmen-Maria; Gonzalez-Rodriguez, Maria-Paloma; Arias-Moliz, Maria-Teresa
Fonte: MEDICINA ORAL S L; VALENCIA Publicador: MEDICINA ORAL S L; VALENCIA
Tipo: Artigo de Revista Científica
ENG
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47.768154%
Objective: To determine the E. faecalis biofilm formation on the surface of five adhesive systems (AS) and its relationship with roughness. Study Design: The formation of E. faecalis biofilms was tested on the surface of four dual-cure AS: AdheSE DC, Clearfil DC Bond, Futurabond DC and Excite DSC and one light-cure antimicrobial AS, Clearfil Protect Bond, after 24 hours of incubation, using the MBEC high-throughput device. Results: E. faecalis biofilms grew on all the adhesives. The least growth of biofilm was on Excite DSC, Clearfil Protect Bond, and the control. Futurabond DC resulted in the greatest roughness and biofilm amount. There was a close relationship between the quantity of biofilm and roughness, except for Clearfil Protect Bond, which showed little biofilm but high roughness. Conclusion: None of the tested AS prevented E. faecalis biofilm formation, although the least quantity was found on the surface of Clearfil Protect Bond.

Action of essential oils from Brazilian native and exotic medicinal species on oral biofilms

Bersan, Salete MF; Galvão, Livia CC; Goes, Vivian FF; Sartoratto, Adilson; Figueira, Glyn M; Rehder, Vera LG; Alencar, Severino M; Duarte, Renata MT; Rosalen, Pedro L; Duarte, Marta CT
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
EN
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Abstract Background Essential oils (EO) obtained from twenty medicinal and aromatic plants were evaluated for their antimicrobial activity against the oral pathogens Candida albicans, Fusobacterium nucleatum, Porphyromonas gingivalis, Streptococcus sanguis and Streptococcus mitis. Methods The antimicrobial activity of the EO was evaluates by microdilution method determining Minimal Inhibitory Concentration. Chemical analysis of the oils compounds was performed by Gas chromatography–mass spectrometry (CG-MS). The most active EO were also investigated as to their actions on the biolfilm formation. Results The most of the essential oils (EO) presented moderate to strong antimicrobial activity against the oral pathogens (MIC - Minimal Inhibitory Concentrations values between 0.007 and 1.00 mg/mL). The essential oil from Coriandrum sativum inhibited all oral species with MIC values from 0.007 to 0.250 mg/mL, and MBC/MFC (Minimal Bactericidal/Fungicidal Concentrations) from 0.015 to 0.500 mg/mL. On the other hand the essential oil of C. articulatus inhibited 63.96% of S. sanguis biofilm formation. Through Scanning Eletronic Microscopy (SEM) images no changes were observed in cell morphology...

Avaliação in vitro do efeito de lectinas de sementes de Talisia esculenta e Labramia bojeri sobre o biofilme oral; Evaluation of in vitro effects of Talisia esculenta and Labramia bojeri seeds lectins on oral biofilm

Mara Rubea Tinoco Rodrigues de Oliveira
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 12/12/2005 PT
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A medicina natural e complementar, especialmente a fitoterapia, suprem as necessidades em saúde de grande parte da população, particularmente nos países em desenvolvimento. Dentre os fitoterápicos, as lectinas podem ter valia como agentes antiplaca, uma vez que podem estar intimamente relacionadas com a aderência de microrganismos. O objetivo deste estudo foi testar in vitro a capacidade de inibição das lectinas isoladas (TEL - derivada da semente de Talisia esculenta e LABRAMIN - purificada da planta Labramia bojeri), na adesão e crescimento de microrganismos orais (Streptococcus sanguinis, S. mitis, S. oralis, S. mutans, S. sobrinus). A atividade antimicrobiana das duas lectinas foi determinada pelo teste convencional da macrodiluição de caldo, sendo testada as concentrações de 400, 200, 100, 50, 25 µg/mL contra 105 CFU/mL dos microrganismos em estudo. Os tubos foram incubados (10% CO2, 37oC, 18h) e submetidos à leitura de densidade óptica (OD a 600nm). A MBC foi determinada pela adição das amostras de cada tubo em placas de petri contendo agar BHI (10% CO2, 37oC, 18h). Para avaliação de aderência foi feito um ensaio semiquantitativo de aderência em placas de microtitulação de poliestireno, onde foi adicionado 100 ?L de saliva clarificada e incubada por 2h a 37°. Após lavagem com PBS...

Microflora oral e risco de doenças orais numa amostra de crianças portuguesas

Gomes, Veronique
Fonte: Universidade Católica Portuguesa Publicador: Universidade Católica Portuguesa
Tipo: Dissertação de Mestrado
Publicado em /06/2014 POR
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Introdução: A saúde oral é parte da saúde geral, bem-estar e qualidade de vida. Deste modo, o conhecimento da prevalência da cárie dentária na população infantil leva-nos a adotar, cada vez mais, um conjunto de medidas de prevenção, permitindo o controlo, não só dos fatores etiológicos da doença, mas também a diminuição da sua incidência. O conhecimento da microflora oral e a exploração do potencial alcalinizante de algumas bactérias orais, são uma estratégia promissora no que diz respeito à prevenção da cárie dentária. Objetivos: Este estudo visa avaliar, os comportamentos e o estado de saúde oral das crianças participantes. Estudar a associação entre o estado de saúde oral das crianças e a presença de Streptococcus mutans; Streptococcus gordonii e Streptococcus salivarius. Métodos: Foi realizado um estudo epidemiológico observacional transversal com 189 crianças dos 6 aos 10 anos de idade. Foi aplicado um questionário aos pais/encarregados de educação sobre os comportamentos de higiene oral das crianças participantes no estudo. Realizou-se uma observação intraoral que nos permitiu determinar o índice de cpo-d e o CPO-D. Por fim, realizou-se a recolha e análise molecular de biofilme oral das crianças participantes. Resultados: Das crianças participantes 70 (36...

Effect of the Environment on Genotypic Diversity of Actinomyces naeslundii and Streptococcus oralis in the Oral Biofilm

Paddick, James S.; Brailsford, Susan R.; Kidd, Edwina A. M.; Gilbert, Steven C.; Clark, Douglas T.; Alam, Sharmin; Killick, Zoe J.; Beighton, David
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /11/2003 EN
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The genotypic diversity of Actinomyces naeslundii genospecies 2 (424 isolates) and Streptococcus oralis (446 isolates) strains isolated from two sound approximal sites in all subjects who were either caries active (seven subjects) or caries free (seven subjects) was investigated by using the repetitive extragenic palindromic PCR. The plaque from the caries-active subjects harbored significantly greater proportions of mutans streptococci and lactobacilli and a smaller proportion of A. naeslundii organisms than the plaque sampled from the caries-free subjects. These data confirmed that the sites of the two groups of subjects were subjected to different environmental stresses, probably determined by the prevailing or fluctuating acidic pH values. We tested the hypothesis that the microfloras of the sites subjected to greater stresses (the plaque samples from the caries-active subjects) would exhibit reduced genotypic diversity since the sites would be less favorable. We found that the diversity of A. naeslundii strains did not change (χ2 = 0.68; P = 0.41) although the proportional representation of A. naeslundii was significantly reduced (P < 0.05). Conversely, the diversity of the S. oralis strains increased (χ2 = 11.71; P = 0.0006) and the proportional representation of S. oralis did not change. We propose that under these environmental conditions the diversity and number of niches within the oral biofilm that could be exploited by S. oralis increased...

Role of Urease Enzymes in Stability of a 10-Species Oral Biofilm Consortium Cultivated in a Constant-Depth Film Fermenter

Shu, Man; Browngardt, Christopher M.; Chen, Yi-Ywan M.; Burne, Robert A.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /12/2003 EN
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Using a 10-species oral biofilm consortium and defined mutants, we show that high-level capacity to generate ammonia from a common salivary substrate is needed to maintain community diversity. This model appears to be suitable for the study of the effects of individual genetic determinants on the ecology of oral biofilms.

Rapid Succession within the Veillonella Population of a Developing Human Oral Biofilm In Situ

Palmer, Robert J.; Diaz, Patricia I.; Kolenbrander, Paul E.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /06/2006 EN
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47.434854%
Streptococci are the primary component of the multispecies oral biofilm known as supragingival dental plaque; they grow by fermentation of sugars to organic acids, e.g., lactic acid. Veillonellae, a ubiquitous component of early plaque, are unable to use sugars; they ferment organic acids, such as lactate, to a mixture of shorter-chain-length acids, CO2, and hydrogen. Certain veillonellae bind to (coaggregate with) streptococci in vitro. We show that, between 4 and 8 hours into plaque development, the dominant strains of Veillonella change in their phenotypic characteristics (coaggregation and antibody reactivity) as well as in their genotypic characteristics (16S RNA gene sequences as well as strain level fingerprint patterns). This succession is coordinated with the development of mixed-species bacterial colonies. Changes in community structure can occur very rapidly in natural biofilm development, and we suggest that this process may influence evolution within this ecosystem.

Association of Systemic Oxidative Stress with Suppressed Serum IgG to Commensal Oral Biofilm and Modulation by Periodontal Infection

Singer, R.E.; Moss, K.; Beck, J.D.; Offenbacher, S.
Fonte: Mary Ann Liebert, Inc. Publicador: Mary Ann Liebert, Inc.
Tipo: Artigo de Revista Científica
Publicado em /12/2009 EN
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To assess the impact of systemic oxidative stress on humoral immune responses, we examined the relation between levels of serum 8-isoprostane and serum IgG antibodies against 17 microorganisms in the commensal oral biofilm among the ARIC population of community-dwelling adults (n = 4,717). Bivariately, serum 8-isoprostane was associated with age, race/center, education, smoking, serum triglycerides, and the extent of periodontal disease severity. Total IgG antibody directed to the oral biofilm was significantly associated with race/center, hypertension, triglycerides, periodontal disease severity, plaque, and serum 8-isoprostane. In multivariate models, the highest quartile of increased 8-isoprostane displayed marked reductions (44%) in biofilm IgG antibody in contrast to small increases in total IgG antibody level for the highest quartiles of oral bacterial burden or periodontal disease severity (19 and 12%, respectively; p < 0.0001). Increased 8-isoprostane was associated with decreased total IgG antibody (p < 0.0001) in subjects with or without extensive periodontal disease and/or biofilm and with suppression of IgG responses across the entire biofilm composition. Increased systemic oxidative stress is associated with a generalized decrease of serum IgG antibody responses to the oral biofilm. Levels of oral microbial burden...

Antimicrobial Penetration and Efficacy in an In Vitro Oral Biofilm Model ▿ †

Corbin, Audrey; Pitts, Betsey; Parker, Albert; Stewart, Philip S.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /07/2011 EN
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The penetration and overall efficacy of six mouthrinse actives was evaluated by using an in vitro flow cell oral biofilm model. The technique involved preloading biofilm cells with a green fluorescent dye that leaked out as the cells were permeabilized by a treatment. The loss of green color, and of biomass, was observed by time-lapse microscopy during 60 min of treatment under continuous flow conditions. The six actives analyzed were ethanol, sodium lauryl sulfate, triclosan, chlorhexidine digluconate (CHX), cetylpyridinium chloride, and nisin. Each of these agents effected loss of green fluorescence throughout biofilm cell clusters, with faster action at the edge of a cell cluster and slower action in the cluster center. The time to reach half of the initial fluorescent intensity at the center of a cell cluster, which can be viewed as a combined penetration and biological action time, ranged from 0.6 to 19 min for the various agents. These times are much longer than the predicted penetration time based on diffusion alone, suggesting that anti-biofilm action was controlled more by the biological action time than by the penetration time of the active. None of the agents tested caused any removal of the biofilm. The extent of fluorescence loss after 1 h of exposure to an active ranged from 87 to 99.5%...

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Klug, Barbara; Rodler, Claudia; Koller, Martin; Wimmer, Gernot; Kessler, Harald H.; Grube, Martin; Santigli, Elisabeth
Fonte: MyJove Corporation Publicador: MyJove Corporation
Tipo: Artigo de Revista Científica
Publicado em 20/10/2011 EN
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Confocal laser scanning microscopy (CLSM) of natural heterogeneous biofilm is today facilitated by a comprehensive range of staining techniques, one of them being fluorescence in situ hybridization (FISH).1,2 We performed a pilot study in which oral biofilm samples collected from fixed orthodontic appliances (palatal expanders) were stained by FISH, the objective being to assess the three-dimensional organization of natural biofilm and plaque accumulation.3,4 FISH creates an opportunity to stain cells in their native biofilm environment by the use of fluorescently labeled 16S rRNA-targeting probes.4-7,19 Compared to alternative techniques like immunofluorescent labeling, this is an inexpensive, precise and straightforward labeling technique to investigate different bacterial groups in mixed biofilm consortia.18,20 General probes were used that bind to Eubacteria (EUB338 + EUB338II + EUB338III; hereafter EUBmix),8-10 Firmicutes (LGC354 A-C; hereafter LGCmix),9,10 and Bacteroidetes (Bac303).11 In addition, specific probes binding to Streptococcus mutans (MUT590)12,13 and Porphyromonas gingivalis (POGI)13,14 were used. The extreme hardness of the surface materials involved (stainless steel and acrylic resin) compelled us to find new ways of preparing the biofilm. As these surface materials could not be readily cut with a cryotome...

Taking the Starch out of Oral Biofilm Formation: Molecular Basis and Functional Significance of Salivary α-Amylase Binding to Oral Streptococci

Nikitkova, Anna E.; Haase, Elaine M.; Scannapieco, Frank A.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /01/2013 EN
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47.69662%
α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-binding proteins. The functional significance of α-amylase-binding proteins in oral colonization by streptococci is important for understanding how salivary components influence oral biofilm formation by these important dental plaque species. This review summarizes the results of an extensive series of studies that have sought to define the molecular basis for α-amylase binding to the surface of the bacterium as well as the biological significance of this phenomenon in dental plaque biofilm formation.

In vitro antibacterial effect of carbamide peroxide on oral biofilm

Yao, Chao Shu; Waterfield, John Douglas; Shen, Ya; Haapasalo, Markus; MacEntee, Michael I.
Fonte: Co-Action Publishing Publicador: Co-Action Publishing
Tipo: Artigo de Revista Científica
Publicado em 12/06/2013 EN
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47.55279%
This study compared the effects of carbamide peroxide (CP) and chlorhexidine (CHX) on oral biofilm in vitro. Collagen-coated hydroxyapatite discs were inoculated with subgingival plaque. After 3 weeks, the emergent biofilms were subjected to 1-, 3-, and 10-min exposures of a 1% CHX gel, a 5% CP gel and rinse, and a 10% CP gel and rinse. Subsequently, the biofilms were stained using a two-colour fluorescent dye kit for confocal laser scanning microscopy, and the volume ratio of dead bacteria to all bacteria was analysed. Compared to a non-treated gel control, the active agents killed bacteria on all the discs, with higher concentration and longer exposure times killing more bacteria. The rinse form disrupted the biofilm quicker than the gel form. Overall, 10% CP showed more disruption of biofilm and a greater proportion of killed bacteria than 1% CHX (p<0.05).

Preventing root caries development under oral biofilm challenge in an artificial mouth

Mei, May L.; Chu, Chun H.; Lo, Edward CM.; Samaranayake, Lakshman P.
Fonte: Medicina Oral S.L. Publicador: Medicina Oral S.L.
Tipo: Artigo de Revista Científica
EN
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57.760864%
Objectives: To study the preventive effects of chlorhexidine against root caries under oral biofilm in an artificial mouth. Study Design: Sixteen human tooth-root disks were inoculated with a salivary sample that was produced by mixing the unstimulated saliva of three adults who had no untreated caries. The disks were incubated in an artificial mouth fed with a 5% sucrose solution three times daily for one week. Eight disks received a twice daily rinse of 0.12% chlorhexidine (test group). The other eight disks were rinsed in distilled water (control). The biofilm was then studied with three techniques: colony forming unit (CFU) counting, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The changes in the chemical structure of the root surface were studied using Fourier transform infra-Red spectroscopy. Type-I collagen and proteoglycans on the root surface were quantified using immunocytochemical staining. Results: The log CFU for the test and control groups were 4.21 and 8.27, respectively (p<0.001). The CFU count of Streptococci and Lactobacilli were negligible. Both the SEM and the CLSM showed suppressed bacteria growth in the test group. The log [amide-I: HPO42-] of the test and control groups were 1.11 and 1.93...

Evaluation of Different Methods for Removing Oral Biofilm in Patients Admitted to the Intensive Care Unit

Oliveira, Maria Sonia; Borges, Alvaro Henrique; Mattos, Fernanda Zanol; Semenoff, Tereza Aparecida Della Vedove; Segundo, Alex Semenoff; Tonetto, Mateus Rodrigues; Bandeca, Matheus Coêlho; Porto, Alessandra Nogueira
Fonte: International Society of Preventive and Community Dentistry Publicador: International Society of Preventive and Community Dentistry
Tipo: Artigo de Revista Científica
EN
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47.898457%
Background: The present study aimed to evaluate the different methods for removing oral biofilm in combination with 0.12% chlorhexidine, in patients admitted to the intensive care unit (ICU) of the General University Hospital. Materials and Methods: Initially, the patients were included in the study and underwent periodontal evaluation by means of the visible plaque index (VPI) and gingival bleeding index (GBI). The removal of visible biofilm, by a professional, was carried out using a toothbrush and dental floss, followed by the application of a 0.12% chlorhexidine solution. The patients were included in this randomized and controlled study into four groups (total n = 48), as follows: Chlorhexidine and gauze 12/12 h; chlorhexidine and gauze 24/24 h; chlorhexidine and brushing 12/12 h; chlorhexidine and brushing 24/24 h. The patients underwent the biofilm removal protocol for 7 days and then were subjected to a new clinical evaluation as to VPI and GBI. Data analysis was performed through stratification and arrangement of the records, in order to carry out the associations with health indicators used in the study, and the statistical tests used were Kappa and t-test for independent and paired samples. Results: A decrease in the VPI and GBI values when comparing baseline to the final evaluation for all groups was observed. Conclusion: Based on the methodology...

The intraoral device of overlaid disk-holding splints as a new in situ oral biofilm model

Prada-López, Isabel; Quintas, Víctor; Tomás, Inmaculada
Fonte: Medicina Oral S.L. Publicador: Medicina Oral S.L.
Tipo: Artigo de Revista Científica
Publicado em 01/02/2015 EN
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58.063887%
Objectives: To design a device that allows the formation of in situ oral biofilm with similar characteristics to those from the dental plaque, overcoming the limitations of previous devices. Study Design: The Intraoral Device of Overlaid Disk-holding Splints (IDODS) was designed and manufactured. To test its validity, five healthy adult volunteers wore them for two and four days allowing the biofilm to grow without any type of distortion. After each period, the thickness, vitality and structure of the formed biofilm were measured with a Confocal Laser Scanning Microscope (CLSM) in combination with a dual fluorescence solution. All volunteers filled out a Likert-type questionnaire to evaluate the device. Results: Mean bacterial vitality in the 2- and 4-day biofilms was 71% and 63%, respectively. Mean thicknesses were 21 µm and 28 µm, respectively. There was predominance in the open and heterogeneous structure whose complexity was ascending as the biofilm matured. The results obtained from the questionnaire were 2/5 in the influence in aesthetics, 3.4/5 in comfort, and 5/5 in ease of maintaining oral hygiene and withdrawal from the oral cavity. Conclusions: A biofilm with optimum characteristics was obtained by IDODS. Its use is associated with good aesthetic and comfort results and is absent of functional limitations...

Ex vivo vs. in vivo antibacterial activity of two antiseptics on oral biofilm

Prada-López, Isabel; Quintas, Víctor; Casares-De-Cal, Maria A.; Suárez-Quintanilla, Juan A.; Suárez-Quintanilla, David; Tomás, Inmaculada
Fonte: Frontiers Media S.A. Publicador: Frontiers Media S.A.
Tipo: Artigo de Revista Científica
Publicado em 02/07/2015 EN
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Aim: To compare the immediate antibacterial effect of two application methods (passive immersion and active mouthwash) of two antiseptic solutions on the in situ oral biofilm.

Efeito de cobertura antiaderente no ac??mulo de biofilme em dispositivos ortod??nticos; Effect of accumulation of coverage nonstick biofilm on orthodontic brackets

OLIVEIRA, Adau?? Siegert de
Fonte: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Odontologia; Programa de P??s-Gradua????o em Odontologia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
POR
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The use of orthodontic devices has been associated with an increase in dental biofilm retention. In this study, the effect of hydrophobic coatings, based on (fluorinated or not) organo-silanes, on the reduction of the contact angle and early biofilm retention in orthodontic devices was tested. Two different types of hydrophobic were tested: hydrophobic solution 1 (SH1), obtained by dilution of 2.5% of hexadecyl-triethoxy-silane in ethanol, and hydrophobic solution 2 (SH2), composed of 2.5% of perfluorodecyl-triethoxy-silane diluted in dimethyl sulfoxide. The solutions were applied in ceramic and stainless steel substrates and, via sol-gel process and crosslinking, siloxane networks were formed on the surface of the devices. The effect of the coatings was evaluated in two stages. In the first stage, aiming the characterization of the surface, the water contact angle was analyzed. Stainless steel and polycrystalline alumina plates were prepared, previously applying or not alumina sandblasting as surface treatment. A significant increase in contact angle was observed with the application of both hydrophobic solutions in both substrates, with the sandblasting allowing formation of super-hydrophobic surface in the ceramic substrate (contact angle up to 155o)...

A comprehensive method for determination of fatty acids in the initial oral biofilm (pellicle)

Reich, Marco; Hannig, Christian; Al-Ahmad, Ali; Bolek, Richard; Kümmerer, Klaus
Fonte: The American Society for Biochemistry and Molecular Biology Publicador: The American Society for Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
Publicado em /10/2012 EN
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The acquired pellicle is a tenacious organic layer covering the surface of teeth, protecting the underlying dental hard tissues. Lipids account for about one quarter of the pellicle's dry weight and are assumed to be of considerable importance for their protective properties. Nevertheless, only preliminary information is available about the nature of lipids in the pellicle. Gas chromatography coupled with electron impact ionization mass spectrometry was used to establish a convenient analytical protocol in order to obtain a qualitative and quantitative characterization of a wide range of FAs (C12–C22). In situ biofilm formation was performed on bovine enamel slabs mounted on individual splints carried by 10 subjects. A modified Folch extraction procedure was adopted to extract the lipids from the detached pellicle, followed by transesterification to fatty acid methyl esters using methanol and concentrated hydrochloric acid. Tridecanoic and nonadecanoic acid were used as internal standards suitable and reliable for robust, precise and accurate measurements. The present study demonstrates, for the first time, a procedure based on a combination of innovative specimen generation and convenient sample preparation with sensitive GC-MS analysis for the determination of the fatty acid profile of the initial oral biofilm.