Página 1 dos resultados de 2027 itens digitais encontrados em 0.005 segundos

Uso do azul de metileno na reversão de vasoplegia refratária ao uso de catecolaminas após bypass aortobifemoral; Use of methylene blue in the reversal of vasoplegia refractory to the use of catecholamines after aortobifemoral bypass

SANTOS, Mariana Raphaela Garcia de Araújo dos; SILVA, Luis Gustavo Campos da; EVORA, Paulo Roberto Barbosa; PICCINATO, Carlos Eli; JOVILIANO, Edwaldo Edner
Fonte: Sociedade Brasileira de Angiologia e de Cirurgia Vascular (SBACV) Publicador: Sociedade Brasileira de Angiologia e de Cirurgia Vascular (SBACV)
Tipo: Artigo de Revista Científica
POR
Relevância na Pesquisa
68.332227%
Há evidências de que o óxido nítrico (NO) tem importância na vasodilatação associada a reações vasoplégicas. O objetivo deste relato de caso é documentar um caso de vasoplegia refratária ao uso de catecolaminas após bypass aortobifemoral revertida com o uso de azul de metileno. Mulher, 50 anos, submetida a bypass aortobifemoral. Sem comorbidades. Saiu de sala cirúrgica extubada, estável e com pulsos distais presentes. Duas horas após a cirurgia, evoluiu com choque circulatório. Iniciada noradrenalina e investigadas causas de choque. Manteve-se com necessidades crescentes de aminas e parâmetros estáveis. No sexto dia pós-operatório, com a hipótese de vasoplegia refratária, optou-se pelo uso do azul. Resposta imediata, com queda nos níveis de aminas, sendo desligada a noradrenalina no dia seguinte. O azul de metileno inibe a guanilato ciclase, produtora de guanosina monofosfato cíclico. Especula-se que haja um sinergismo entre essas drogas, já que a sua associação permite a atuação do sistema adenosina monofosfato.; There is evidence that the nitric oxide plays an important role in the vasodilation associated with vasoplegic reactions. The objective of this case report is to document a case of catecholamine-refractory shock after aortobifemoral bypass treated with methylene blue. A 50-year-old woman was admitted for aortobifemoral bypass graft surgery. She did not present with any comorbidities. At the end of the surgery...

Is the poly (methylene blue)-modified glassy carbon electrode an adequate electrode for the simple detection of thiols and amino acid-based molecules?

Costa Marinho, Maria Ines; Cabral, Murilo Feitosa; Mazo, Luiz Henrique
Fonte: ELSEVIER SCIENCE SA; LAUSANNE Publicador: ELSEVIER SCIENCE SA; LAUSANNE
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
68.24539%
This paper describes the preparation, characterization, and use of poly (methylene blue) (PMB)-modified glassy carbon electrodes (GCE) (GCE-PMB) in the detection of the thiols L-cysteine (L-CySH) and N-acetyl cysteine (Acy), and the herbicide glyphosate (GLYP) in pH 5.3 aqueous solution. The polymer film prepared by electropolymerization showed different characteristics such as robustness, stability, and redox properties satisfactorily. The surface coverage concentration (Gamma) of PMB was found to be 7.90 x 10(-9) - mol cm(-2). Moreover, we observed strong adhesion of the polymer film to the electrode surface. The results using GCE-PMB as a sensor indicated that this modified electrode exhibited electrocatalytic activity toward the detection of thiols and glyphosate in 0.1 mol L-1 KO (pH 5.3). Meanwhile, strong adsorption of the analytes on the GCE-PMB electrodes was also observed. Otherwise, using a low concentration (1 x 10(-4) mol L-1) of L-cysteine and N-acetyl cysteine and 8.9 x 10(-6) mol L-1 of glyphosate, separately, it was possible to observe a well-defined electrochemical response, thus providing an opportunity to further understand the applicability of PMB as a sensor for amino acid-based molecules. (C) 2012 Elsevier B.V. All rights reserved.; CAPES; CAPES [PNPD/CAPES 0316083]; FINEP; FINEP

Effect of photodynamic therapy with different formulations of methylene blue in teeth contaminated by Enterococcus faecalis

Souza, Eliana Barbosa de; Simionato, Maria Regina Lorenzetti; Marques, Jose Luiz da Silva Lage; Antoniazzi, Joao Humberto
Fonte: OMICS Publishing Group; Los Angeles Publicador: OMICS Publishing Group; Los Angeles
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
68.527285%
The aim of this study was to compare the disinfection of dentine using photodynamic therapy with methylene blue in different formulations. Thirty bovine teeth roots were autoclaved and incubated with a suspension of Enterococcus faecalis. The specimen were randomly divided into three groups: G1, the roots were filled with 10 mM methylene blue dissolved in water; G2, the roots were filled with 10 mM methylene blue dissolved in a mixture of glycerol: ethanol: water; G3, roots filled with 100 mM methylene blue dissolved in water. The groups were irradiated with a 660 nm diode laser with an output power of 100 mW for 4 min, energy density of 850 J/cm2 and after this procedure, the sensitizer was removed and microbial samples were collected from within the root canals. The samples were plated on mEnterococcus to count the colony-forming units (CFU/mL). The means were: Group 1=513×103, Group 2=1431×103 and Group 3=2.96×103. The statistical analysis detected higher disinfection achieved by G3 when compared with groups G1 and G2, and no significant difference between the groups G1 and G2 (P>0.05). The increase of the concentration of methylene blue dye achieved higher disinfection in photodynamic therapy.

Proposta de um método de ensaio para determinação do volume de azul de metileno adsorvido pela fração fina de solos tropicais ; Proposal of a test method for determination of the volume of methylene blue adsorbed by the fine fraction of tropical soils

Bonini, Andréa de Oliveira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 25/11/2005 PT
Relevância na Pesquisa
68.593013%
No Brasil utiliza-se, como técnica de caracterização da fração fina dos solos tropicais, entre outras, o ensaio de adsorção de azul de metileno pelo método da mancha. No entanto, para alguns solos, há dificuldade na identificação do ponto de viragem, ou seja, quando há excesso de corante não adsorvido pelo solo. Assim, apresenta-se neste trabalho uma técnica alternativa para facilitar a determinação do volume de azul de metileno adsorvido por um solo, cujo método é baseado na adição em excesso de azul de metileno à amostra e na titulação de óxido-redução volumétrica (redox) do azul de metileno que não é adsorvido pelo solo. Para a avaliação do ensaio de adsorção de azul de metileno pelo método redox foram ensaiadas 85 amostras de solos e, a partir dos resultados, foram estabelecidos seus graus de atividade que, por sua vez, foram correlacionados com a previsão de comportamento dos solos dada pela MCT. Conclui-se que o procedimento de ensaio proposto é simples e a percepção do ponto de viragem na titulação do excesso de azul de metileno pelo agente oxidante permanganato de potássio é fácil para maioria dos solos, além de apresentar uma boa concordância com os resultados fornecidos pela classificação MCT...

Variáveis hemodinâmicas, gasométricas e imunomoleculares em pacientes submetidos a revascularização miocárdica com uso de azul de metileno: estudo clínico e prospectivo; Hemodynamic, gasometric and immunomolecular responses to coronary artery bypass grafting with methylene blue infusion: a randomized and prospective study

Ribeiro, Nilzo Augusto Mendes
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 12/03/2004 PT
Relevância na Pesquisa
68.332227%
O objetivo do presente estudo, prospectivo e randomizado, é avaliar comparativamente as respostas hemodinâmicas, laboratoriais, imunomoleculares e clínicas, em dois grupos de pacientes submetidos a cirurgia de revascularização miocárdica com extracorpórea utilizando-se, em um deles infusão de azul de metileno durante o período intraoperatório. As variáveis hemodinâmicas estudadas foram: FC, PAE, PVC, DC, RVS, RVP, PAPM, PASS, PASD e PASM. Os dados laboratoriais avaliados foram: gasometria arterial, creatinina, leucócitos, bastões, neutrófilos, eosinífilos, basófilos, linfócitos, Ht, Hb, paquetas, Na+ e K+. Os marcadores imunomoleculares estudados foram: IL - 1ß, IL-6, IL-8, IL-10, IL-12 p40, TNF alfa e NO. Os dados clínicos avaliados foram: idade, sexo, raça, tabagismo, hipertensão, diabetes, angina estável, angina instável, infarto prévio, tempo de CEC, tempo de pinçamento aórtico, número de pontes realizadas, urina azulada, fezes azuladas, diarréia, náuseas, cefaléia, tontura, disúria, astenia e dispnéia. As variáveis citadas foram obtidas em todos ou em parte dos períodos seguintes: antes da indução, terceira hora, sexta hora, 24ª hora e 48ª hora após o término da CEC. Ocorreram diferenças estatisticamente significativas na resistência vascular sistêmica...

Caracterização da fração fina de solos tropicais através da adsorção de azul de metileno; The use of methylene blue for tropical soil fine fraction characterization

Fabbri, Glauco Tulio Pessa
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 13/04/1994 PT
Relevância na Pesquisa
68.332227%
Esta tese apresenta uma proposta para caracterização da fração fina de solos tropicais, baseada na superfície específica dos argilo-minerais nela presentes. A partir da superfície específica, determinada pela adsorção do corante azul de metileno, são estabelecidos graus de atividade para os solos analisados. Essa atividade é então comparada com a previsão de comportamento dos solos, obtida da classificação MCT (Miniatura, Compactado e Tropical). Para tanto, é feita uma avaliação dos fatores que influem nos ensaios de adsorção de azul de metileno (método da mancha) e, são comparados, exaustivamente, para 297 amostras de solos, os resultados da adsorção de azul de metileno com a previsão de comportamento dada pela MCT. Conclui-se que a utilização da adsorção de azul de metileno é promissora para a caracterização da fração fina de solos tropicais, permitindo estabelecer, com razoável segurança, a atividade dos argilo-minerais presentes e que existe uma boa concordância entre os resultados da adsorção de azul de metileno e os fornecidos pela classificação MCT.; A strategy to identify the thin fraction of tropical soils, based on the specific surfaces of its clay minerals, is presented in this work. The specific surface...

Avaliação dos efeitos do azul de metileno fotoativado no modelo experimental do Tumor de Walker 256.; Evaluation of the effects of methylene blue photoactivated in experimental model of Tumor Walker 256.

Petrellis, Maria Carla
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 01/09/2014 PT
Relevância na Pesquisa
68.332227%
A TFD é considerada uma nova terapia minimamente invasiva destinada ao tratamento e destruição seletiva de diversos tipos de cânceres. Objetivo foi avaliar se os efeitos do azul de metileno fotoativado podem desencadear processos inflamatórios interferindo no desenvolvimento e na progressão tumoral. Os resultados demonstraram que o grupo tratado com 0.1% de azul de metileno+1J provocou um aumento estatísticamente significativo quando comparado em relação aos diferentes grupos tratados nos níveis e na expressão gênica dos diferentes marcadores inflamatórios, na geração EROS e MPO. Análise histológica complementou com os resultados anteriores, indicando que neste grupo há alterações morfológicas representadas por áreas de necrose na massa tumoral sólida com presença neutrofílica. Concluímos que há indícios que o tratamento 0.1% azul de metileno+ 1J foi capaz de gerar efeitos citotóxicos que por consequência aumentou a expressão dos mediadores inflamatórios promovendo inflamação e finalmente induzindo morte celular.; PDT is considered a new minimally invasive therapy for the treatment and selective destruction of various types of cancers. The objective was to evaluate the effects of methylene blue light activated may trigger inflammatory processes interfering with the development and tumor progression. The results showed that the group treated with 0.1 % methylene blue +1 J caused a statistically significant increase levels and gene expression of different inflammatory markers in ROS generation and MPO when compared at the different treat groups. Histological analysis complemented with previous results...

Comparison of the photodynamic fungicidal efficacy of methylene blue, toluidine blue, malachite green and low-power laser irradiation alone against Candida albicans

Souza, Rodrigo C.; Junqueira, Juliana Campos; Rossoni, Rodnei D.; Pereira, Cristiane A.; Munin, Egberto; Jorge, Antonio O. C.
Fonte: Springer London Ltd Publicador: Springer London Ltd
Tipo: Conferência ou Objeto de Conferência Formato: 385-389
ENG
Relevância na Pesquisa
68.35893%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 06/54896-1; This study was to evaluate specific effects of photodynamic therapy (energy density 15.8 J/cm(2), 26.3 J/cm(2) and 39.5 J/cm(2)) using methylene blue, toluidine blue and malachite green as photosensitizers and low-power laser irradiation on the viability of Candida albicans. Suspensions of C. albicans containing 10(6) cells/ml were standardized in a spectrophotometer. For each dye, 120 assays, divided into four groups according to the following experimental conditions, were carried out: laser irradiation in the presence of the photosensitizer; laser irradiation only; treatment with the photosensitizer only; no exposure to laser light or photosensitizer. Next, serial dilutions were prepared and seeded onto Sabouraud dextrose agar for the determination of the number of colony-forming units per milliliter (CFU/ml). The results were subjected to analysis of variance and the Tukey test (P < 0.05). Photodynamic therapy using the photosensitizers tested was effective in reducing the number of C. albicans.. The number of CFU/ml was reduced by between 0.54 log(10) and 3.07 log(10) and depended on the laser energy density used. Toluidine blue, methylene blue and malachite green were effective photosensitizers in antimicrobial photodynamic therapy against C. albicans...

Adsorption and catalytic properties of SiO2/Bi2S3 nanocomposites on the methylene blue photodecolorization process

Albuquerque, Rita; Neves, Márcia C.; Mendonça, Maria H.; Trindade, Tito; Monteiro, Olinda C.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
68.578906%
The decolorization of aqueous solutionsmethylene blue (C.I. Basic Blue 9), due to the presence of nanocrystalline Bi2S3, supported on SiO2 submicron particles,was investigated here. For this decolorization process, two distinct characteristics, though related, associated to the role of SiO2/Bi2S3 were identified: (i) high methylene blue adsorption capability and (ii) photocatalytic activity to methylene blue photodecolorization. Effects of experimental parameters on the decolorization process, such as methylene blue and nanocomposite concentrations, pH and Bi2S3 particle size were investigated. The maximum adsorption ability of the SiO2/Bi2S3 was approximately 15.6mg methylene blue per gram. The complete decolorization of a 16ppm organic dye solution can be achieved, by an adsorption process, in an extremely short time (less than 5 min), using 1.6 g/L of SiO2/Bi2S3 nanocomposite. The study of the decolorization of the dye by an adsorption–photoassisted decolorization processwas carried out by irradiation of a suspension prepared with 100mL of methylene blue solution (8 ppm) and 50mg of SiO2/Bi2S3. In these conditions the complete decolorization of the dye, adsorbed and in the solution, was achieved in 40 min.; FCT - SFRH/BPD/14554/2003; FEDER - POCI/QUI/59615/2004

Methylene blue vital staining for Trypanosoma cruzi trypomastigotes and epimastigotes

Ferreira,Cláudio S.; Bezerra,Rita C.; Pinheiro,Ariadne A.
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2006 EN
Relevância na Pesquisa
68.332227%
The morphological identification of Trypanosoma cruzi is currently considered to have a high specificity, but its sensitivity, which depends on the volume of the sample examined, is rather low. Trypanosome developmental stages suspended in blood, reduviid feces, and culture media are routinely searched for by means of fresh film examination (about 2 µL). High speed centrifugation of blood samples separates the buffy coat, where most trypomastigotes concentrate. As the parasites are transparent and colorless, their detection is mostly dependent on their motility. The fluorescent vital stain acridine orange has been used to enhance image contrast, as exemplified by the QBC (Quantitative Buffy Coat) technique. Staining blood, buffy coat, reduviid feces, and culture media samples with methylene blue (also a vital dye) is a means of producing sharp, well contrasted images of motile or non-motile T. cruzi developmental stages, only standard laboratory microscopes being required. Slides previously coated with a thin layer of methylene blue are used to stain fresh blood films. Photomicrographs exemplify the results of methylene blue staining applied to living and fixed parasites.

Ionic imbalance and lack of effect of adjuvant treatment with methylene blue in the hamster model of leptospirosis

Santos,Cleiton Silva; Azevedo,Everton Cruz de; Soares,Luciane Marieta; Carvalho,Magda Oliveira Seixas; Santos,Andreia Carvalho dos; Chagas Junior,Adenizar Delgado das; Silva,Caroline Luane Rabelo da; Chagas,Ursula Maira Russo; Reis,Mitermayer Galvao dos;
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2013 EN
Relevância na Pesquisa
68.332227%
Leptospirosis in humans usually involves hypokalaemia and hypomagnesaemia and the putative mechanism underlying such ionic imbalances may be related to nitric oxide (NO) production. We previously demonstrated the correlation between serum levels of NO and the severity of renal disease in patients with severe leptospirosis. Methylene blue inhibits soluble guanylyl cyclase (downstream of the action of any NO synthase isoforms) and was recently reported to have beneficial effects on clinical and experimental sepsis. We investigated the occurrence of serum ionic changes in experimental leptospirosis at various time points (4, 8, 16 and 28 days) in a hamster model. We also determined the effect of methylene blue treatment when administered as an adjuvant therapy, combined with late initiation of standard antibiotic (ampicillin) treatment. Hypokalaemia was not reproduced in this model: all of the groups developed increased levels of serum potassium (K). Furthermore, hypermagnesaemia, rather than magnesium (Mg) depletion, was observed in this hamster model of acute infection. These findings may be associated with an accelerated progression to acute renal failure. Adjuvant treatment with methylene blue had no effect on survival or serum Mg and K levels during acute-phase leptospirosis in hamsters.

The protective effect of methylene blue in lungs, small bowel and kidney after intestinal ischemia and reperfusion

Greca,Fernando Hintz; Gonçalves,Neuza Maria Ferraz de Mello; Souza Filho,Zacarias Alves de; Noronha,Lúcia de; Silva,Ruy Fernando Kuenzer Caetano da; Rubin,Michel Risnic
Fonte: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia Publicador: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2008 EN
Relevância na Pesquisa
68.332227%
PURPOSE: To study the role of methylene blue as an inhibitor of superoxide production by xanthine oxidase. METHODS: Thirty-two Wistar rats were divided into 2 groups of 16 animals: the control group and the experimental group. All were submitted to a laparotomy for the occlusion of the cranial mesenteric artery during 60 minutes. The reperfusion was confirmed by the pulsation of the artery after the release of the temporary ligature and color change of the intestines. In the animals of the control group, 2 ml of saline were injected in the peritoneal cavity and in the animals of the experimental group, 2 ml of methylene blue were injected in the peritoneal cavity. After reperfusion for 4 hours, the animals were then sacrificed. The lungs were excised from all 32 rats. Simultaneously, the small intestine and kidneys were ressected in 20 animals (10 from the control group and 10 from the experimental group). Samples of the organs were taken to evaluate the action of xanthine-oxidase, for histopathology studies and for characterization of the edema. RESULTS: In the animals of the experimental group, the inflammatory lesion as well as the edema in the lung was greater than in the control group. The intestinal and renal lesions were similar in both groups...

The influence of methylene blue on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats

Miranda,Eron Fabio; Greca,Fernando Hintz; Noronha,Lucia; Kotze,Luiz Roberto; Rubin,Michel Risnic
Fonte: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia Publicador: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2010 EN
Relevância na Pesquisa
68.593013%
PURPOSE: To investigate the influence of methylene blue, on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats. METHODS: Forty-five rats divided into the following three groups were used: control (G1); ischemia without methylene blue (G2); and ischemia with methylene blue (G3). A laparotomy was performed and the cranial mesenteric artery isolated. Whereas the cranial artery was temporarily occluded for 45 minutes in groups G2 and G3, prior to enterotomy and intestinal anastomosis, in group G1 the enterotomy and intestinal anastomosis were performed without prior lesion. Afterwards, 2mL of 0.5% methylene blue were instilled in the peritoneal cavities of the animals in group G3, and 2mL of isotonic saline solution in the peritoneal cavities of the animals in group G2. After the reperfusion, an enterectomy and intestinal anastomosis were performed. After the animals had been sacrificed on the seventh day after the operation, the abdominal cavity was examined by resection of a segment of the intestine containing the anastomosis in order to measure its strength and for histopathological examination. RESULTS: Free fluid or abscesses in the peritoneal cavity were rare. When inflammation was analyzed, the group subjected to ischemia without methylene blue had a higher score for mononuclear cells (p=0.021) and granulation tissue (p=0.044). No significant difference was observed in the density of type I or type III collagens. CONCLUSION: The methylene blue did not show beneficial effect on the healing of intestinal anastomoses subjected to ischemia and reperfusion in rats.

Blockade of the action of nitric oxide in human septic shock increases systemic vascular resistance and has detrimental effects on pulmonary function after a short infusion of methylene blue

Weingartner, Roger; Oliveira, E.; Oliveira, Elizabeth dos Santos Boos de; Sant'Anna, Urbano Leonel; Oliveira, Rodrigo Pereira de; Azambuja, L. A.; Friedman, Gilberto
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
68.593013%
To investigate the role of nitric oxide in human sepsis, ten patients with severe septic shock requiring vasoactive drug therapy and mechanical ventilation were enrolled in a prospective, open, non-randomized clinical trial to study the acute effects of methylene blue, an inhibitor of guanylate cyclase. Hemodynamic and metabolic variables were measured before and 20, 40, 60, and 120 min after the start of a 1-h intravenous infusion of 4 mg/kg of methylene blue. Methylene blue administration caused a progressive increase in mean arterial pressure (60 [55-70] to 70 [65-100] mmHg, median [25-75th percentiles]; P<0.05), systemic vascular resistance index (649 [479-1084] to 1066 [585-1356] dyne s-1 cm-5 m-2; P<0.05) and the left ventricular stroke work index (35 [27-47] to 38 [32-56] g m-1 m-2; P<0.05) from baseline to 60 min. The pulmonary vascular resistance index increased from 150 [83-207] to 186 [121-367] dyne s-1 cm-5 m-2 after 20 min (P<0.05). Mixed venous saturation decreased from 65 [56-76] to 63 [55-69]% (P<0.05) after 60 min. The PaO2/FiO2 ratio decreased from 168 [131-215] to 132 [109-156] mmHg (P<0.05) after 40 min. Arterial lactate concentration decreased from 5.1 ± 2.9 to 4.5 ± 2.1 mmol/ l, mean ± SD (P<0.05) after 60 min. Heart rate...

In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms

Oliveira,Bruna Paloma de; Lins,Carla Cabral dos Santos Accioly; Diniz,Fátima Alves; Melo,Liliane Lima; Castro,Célia Maria Machado Barbosa de
Fonte: Faculdade de Odontologia de Piracicaba - UNICAMP Publicador: Faculdade de Odontologia de Piracicaba - UNICAMP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2014 EN
Relevância na Pesquisa
68.332227%
AIM: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). METHODS: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (10 8 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane's comparisons. RESULTS: The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). CONCLUSIONS: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.

Blockade of the action of nitric oxide in human septic shock increases systemic vascular resistance and has detrimental effects on pulmonary function after a short infusion of methylene blue

Weingartner,R.; Oliveira,E.; Oliveira,E.S.; Sant'Anna,U.L.; Oliveira,R.P.; Azambuja,L.A.; Friedman,G.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/1999 EN
Relevância na Pesquisa
68.593013%
To investigate the role of nitric oxide in human sepsis, ten patients with severe septic shock requiring vasoactive drug therapy and mechanical ventilation were enrolled in a prospective, open, non-randomized clinical trial to study the acute effects of methylene blue, an inhibitor of guanylate cyclase. Hemodynamic and metabolic variables were measured before and 20, 40, 60, and 120 min after the start of a 1-h intravenous infusion of 4 mg/kg of methylene blue. Methylene blue administration caused a progressive increase in mean arterial pressure (60 [55-70] to 70 [65-100] mmHg, median [25-75th percentiles]; P<0.05), systemic vascular resistance index (649 [479-1084] to 1066 [585-1356] dyne s-1 cm-5 m-2; P<0.05) and the left ventricular stroke work index (35 [27-47] to 38 [32-56] g m-1 m-2; P<0.05) from baseline to 60 min. The pulmonary vascular resistance index increased from 150 [83-207] to 186 [121-367] dyne s-1 cm-5 m-2 after 20 min (P<0.05). Mixed venous saturation decreased from 65 [56-76] to 63 [55-69]% (P<0.05) after 60 min. The PaO2/FiO2 ratio decreased from 168 [131-215] to 132 [109-156] mmHg (P<0.05) after 40 min. Arterial lactate concentration decreased from 5.1 ± 2.9 to 4.5 ± 2.1 mmol/l, mean ± SD (P<0.05) after 60 min. Heart rate...

Distribution of Methylene Blue after Injection into the Epidural Space of Anaesthetized Pregnant and Non-Pregnant Sheep

Moll Sánchez, Xavier; García Arnas, Félix; Ferrer, Rosa Isabel; Santos Benito, Laura; Aguilar Catalán, Adrià
Fonte: Universidade Autônoma de Barcelona Publicador: Universidade Autônoma de Barcelona
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em //2014 ENG
Relevância na Pesquisa
68.332227%
The aim of the study was to determine the distribution of different volumes of methylene blue solution injected into the epidural space in anaesthetized pregnant and non-pregnant sheep, to evaluate its cranial distribution and to compare between them. Fifteen pregnant and fifteen non-pregnant sheep were included in the study. Sheep were anaesthetized and received 0.05, 0.1, or 0.2 mL/kg of a lumbosacral epidural solution containing 0.12% methylene blue in 0.9% saline. Thirty minutes after the epidural injection, the ewes were euthanized. The extension of the dye within the epidural space was measured, and the correlation between the volume of the dye injected and the number of stained vertebrae was evaluated. The cranial migration of the dye between pregnant and non-pregnant sheep was also compared. The results show that the volume of methylene blue injected epidurally into pregnant and non-pregnant sheep correlated directly with its cephalic distribution into the epidural space; and a volume of 0.1 mL/kg or 0.2 mL/kg stained up to the first lumbar segment in pregnant and non-pregnant sheep, respectively. Also, the results suggest that the volume of drugs administered into the epidural space of pregnant sheep should be half the volume that would be used in non-pregnant sheep.

Coloração vital com azul de metileno aplicada a tripomastigotas e epimastigotas de Trypanosoma cruzi; Methylene blue vital staining for Trypanosoma cruzi trypomastigotes and epimastigotes

Ferreira, Cláudio S.; Bezerra, Rita C.; Pinheiro, Ariadne A.
Fonte: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo Publicador: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/12/2006 ENG
Relevância na Pesquisa
68.332227%
A identificação morfológica de Trypanosoma cruzi tem alta especificidade, segundo é geralmente aceito; entretanto, sua sensibilidade, dependente do volume da amostra examinada, é baixa. Formas evolutivas de T. cruzi suspensas em sangue, fezes de reduviídeos e meios de cultura são rotineiramente pesquisadas em esfregaços a fresco (cerca de 2 µL). Centrifugação de amostras de sangue a altas velocidades produz a separação do creme leucocitário, onde se concentram as formas tripomastigotas. Em preparações a fresco, a motilidade das formas tripomastigotas e epimastigotas de T. cruzi, protozoário transparente e incolor, facilita sua detecção. Laranja de acridina, corante vital fluorescente, tem sido usada para acentuar o contraste das imagens de parasitas. Disto é exemplo a técnica QBC (Quantitative Buffy Coat). A coloração por meio de azul de metileno (também um corante vital), de amostras de sangue, de fezes de reduviídeos ou de meios de cultura permite obter imagens nítidas e contrastadas de formas evolutivas de T. cruzi com ou sem motilidade. Microscópios de uso geral em laboratórios permitem o exame dos parasitas corados. Uma camada bem delgada de azul de metileno colocada sobre a parte central da lâmina limpa (por meio da evaporação de solução diluída do corante) é usada para corar as preparações a fresco. O aspecto dos parasitas corados em materiais frescos ou previamente fixados pode ser observado em fotomicrografias.; The morphological identification of Trypanosoma cruzi is currently considered to have a high specificity...

Kinetic Study of the Electrochemical Oxidation of Methylene Blue with Pt Electrode

Hassan,M.A. El Hajj; El Jamal,M.M.
Fonte: Sociedade Portuguesa de Electroquímica Publicador: Sociedade Portuguesa de Electroquímica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2012 EN
Relevância na Pesquisa
68.39494%
Kinetic study of the indirect oxidation of methylene blue on Pt electrode in presence of several strong electrolytes is undertaken. Different operating conditions that affected the treatment process were studied in order to find the best conditions. The order with respect to methylene blue is zero order in presence of chloride, but it is second order in presence of bromide. The oxidation rate was affected by current density, halide concentration (KCl, KBr), nature of supporting electrolyte and initial pH. However, the initial dye concentration and temperature did not show a significant effect. The oxidation of methylene blue in presence of iodide, fluoride and sulfate is absent, but it is important in presence of chloride and bromide. The product of the indirect oxidation is the chloronated (bromonated) methylene violet bernthsen.

In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms

Oliveira, Bruna Paloma de; Lins, Carla Cabral dos Santos Accioly; Diniz, Fátima Alves; Melo, Liliane Lima; Castro, Célia Maria Machado Barbosa de
Fonte: UNICAMP/FOP Publicador: UNICAMP/FOP
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ;
Publicado em 13/10/2015 POR
Relevância na Pesquisa
68.332227%
Aim: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). Methods: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (108 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane’s comparisons. Results:The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). Conclusions: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.