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The MX2 macromolecular crystallography beamline: a wiggler X-ray source at the LNLS

GUIMARAES, Beatriz G.; SANFELICI, Lucas; NEUENSCHWANDER, Regis T.; RODRIGUES, Flavio; GRIZOLLI, Walan C.; RAULIK, Marco A.; PITON, James R.; MEYER, Bernd C.; NASCIMENTO, Alessandro Silva; POLIKARPOV, Igor
Fonte: WILEY-BLACKWELL PUBLISHING, INC Publicador: WILEY-BLACKWELL PUBLISHING, INC
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
697.85516%
The Brazilian Synchrotron Light Laboratory [Laboratorio Nacional de Luz Sincrotron (LNLS), Campinas, SP, Brazil] is the first commissioned synchrotron light source in the southern hemisphere. The first wiggler macromolecular crystallography beamline (MX2) at the LNLS has been recently constructed and brought into operation. Here the technical design, experimental set-up, parameters of the beamline and the first experimental results obtained at MX2 are described. The beamline operates on a 2.0 T hybrid 30-pole wiggler, and its optical layout includes collimating mirror, Si( 111) double-crystal monochromator and toroidal bendable mirror. The measured flux density at the sample position at 8.7 eV reaches 4.8 x 10(11) photons s(-1) mm(-2) (100 mA)(-1). The beamline is equipped with a MarResearch Desktop Beamline Goniostat (MarDTB) and 3 x 3 MarMosaic225 CCD detector, and is controlled by a customized version of the Blu-Ice software. A description of the first X-ray diffraction data sets collected at the MX2 LNLS beamline and used for macromolecular crystal structure solution is also provided.

A powder X-ray diffraction method for detection of polyprenylated benzophenones in plant extracts associated with HPLC for quantitative analysis

MARTINS, Felipe T.; SANTOS, Marcelo H. dos; COELHO, Carla P.; BARBOSA, Luiz C. A.; DIAS, Gizelly C.; FRACCA, Monica P.; NEVES, Person P.; STRINGHETA, Paulo C.; DORIGUETTO, Antonio C.
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
709.76625%
A robust, direct, rapid and non-destructive X-ray diffraction crystallography method to detect the polyprenylated benzophenones 7-epi-clusianone (1) and guttiferone A (2) in extracts from Garcinia brasiliensis is presented. Powder samples of benzophenones 1 and 2, dried hexane extracts from G. brasiliensis seeds and fruit`s pericarp, and the dried ethanolic extract from G. brasiliensis seeds were unambiguously characterized by powder X-ray diffractometry. The calculated X-ray diffraction peaks from crystal structures of analytes 1 and 2, previously determined by single-crystal X-ray diffraction technique, were overlaid to those of the experimental powder diffractograms, providing a practical identification of these compounds in the analyzed material and confirming the pure contents of the powder samples. Using the X-ray diffraction crystallography method, the studied polyprenylated benzophenones were selectively and simultaneously detected in the extracts which were mounted directly on sample holder. In addition, reference materials of the analytes were not required for analyses since the crystal structures of the compounds are known. High performance liquid chromatography analyses also were comparatively carried out to quantify the analytes in the same plant extracts showing to be in agreement with X-ray diffraction crystallography method. (C) 2010 Elsevier B.V. All rights reserved.; FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Brazilian Research Council CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); FAPEMIG (Fundacao de Amparo Pesquisa do Estado de Minas Gerais)[APQ-2011-5.02/07]; Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG); Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG); FAPEMIG (Fundacao de Amparo Pesquisa do Estado de Minas Gerais)[APQ-6010-5.02/07]; FAPEMIG (Fundacao de Amparo Pesquisa do Estado de Minas Gerais)[APQ-02685-09]; Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG); Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG); FAPEMIG (Fundacao de Amparo Pesquisa do Estado de Minas Gerais)[CAG-00028/01]; FINEP (Financiadora de Estudos e Projetos)[0134/08]; Financiadora de Estudos e Projetos (FINEP); FINEP (Financiadora de Estudos e Projetos)[0336/09]; Financiadora de Estudos e Projetos (FINEP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)

Crystal and molecular structures of three 2-sulphur-substituted cyclohexanones studied by X-ray crystallography and by ab initio molecular orbital calculations

ZUKERMAN-SCHPECTOR, Julio; VINHATO, Elisangela; OLIVATO, Paulo R.; RODRIGUES, Alessandro; COLLE, Maurizio Dal; CERQUEIRA JR., Carlos R.; ARMAN, Hadi D.; TIEKINK, Edward R. T.
Fonte: OLDENBOURG VERLAG Publicador: OLDENBOURG VERLAG
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
694.7046%
The conformational features of three 2-sulphur-substituted cyclohexanone derivatives, which differ in the number of sulphur-bound oxygen atoms, i.e. zero (I), one (II) and two (III), were investigated by single crystal X-ray crystallography and geometry optimized structures determined using Hartree-Fock method. In each of (I)-(III) an intramolecular S center dot center dot center dot O(carbonyl) interaction is found with the magnitude correlated with the oxidation state of the sulphur atom, i.e. 2.838(3) angstrom in (I) to 2.924(2) angstrom in (II) to 3.0973(18) angstrom in (III). There is an inverse relationship between the strength of this interaction and the magnitude of the carbonyl bond. The supramolecular aggregation patterns are primarily determined by C-H center dot center dot center dot O contacts and are similarly influenced by the number of oxygen atoms in the molecular structures. Thus, a supramolecular chain is found in the crystal structure of (I). With an additional oxygen atom available to participate in C-H center dot center dot center dot O interactions, as in (II), a two-dimensional array is found. Finally, a three-dimensional network is found for (III). Despite there being differences in conformations between the experimental structures and those calculated in the gas-phase...

Introdução aos métodos de determinação de estruturas cristalinas por difração de raios-X: complexos de Rutênio.; Introduction to crystal structure determination methods by X-ray diffraction: ruthenium complexes.

Fontes, Marcos Roberto de Mattos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 25/03/1992 PT
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Esta dissertação consiste de duas partes: Parte I: Descrição teórica suscinta dos fundamentos da cristalografia de raios-X. Parte II: Resolução de quatro estruturas cristalinas, três complexos de rutênio (capítulos V, VI, VII) e um ligante comumente encontrado em complexos de rutênio, o C28H28O2P2. As estruturas resolvidas foram: 1) C28H28O2P2, Mr= 458.48, triclínico, PI a= 5.826(1), b= 8.862(1), c= 12.517(2)Å, α= 100.29(1), β= 102.67(1), Υ= 104.22(1)°, V= 592.5(3)޵, Z=1, Dx=1.285g.cm-3, λ(MοKα)=0,71073Å, μ= 2.00cm-1, F(000)=242, T=296K, Rint=0.01, R=0.031, Rw=0.030 para 1390 reflexões independentes observadas [I> 3 ο (I)]. Os átomos P estão a 0.126(1)Å do plano formado pelo grupo (CH2)4-. Os anéis fenil são planares dentro da margem de erro experimental. Os átomos P têm uma configuração tetraédrica distorcida. 2) PyH[RuCl4(CO)Py], Mr=430.11, monoclínico, P21/n, a= 7.821(1), b= 10.337(3), c= 19.763(3)Å, β=93.07(1)°, V= 1595.5(5)޵, Z=4, Dx=1.791g.cm-3, λ(MοKα)=0,71073Å, μ= 14.86cm-1, F(000)=843.9, T=296K, Rint=0.03, R=0.062, Rw=0.063 para 1478 reflexões independentes observadas [I> 3ο (I)]. A estrutura é composta essencialmente por dois planos perpendiculares entre si; um formado pelos quatro átomos de cloro (com ângulo de aproximadamente 90° entre si)...

Determinação da simetria de coordenação de alguns complexos de lantanídeos por difração de raios-x; Coordination symmetry studies in some lanthanide complex by X-ray diffraction

Santos, Carlos de Oliveira Paiva
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 16/08/1983 PT
Relevância na Pesquisa
707.6208%
lantanídeos, visando a determinação da simetria de coordenação ao redor dos íons e sua comparação com prévias previsões espectroscópicas. As medidas de difração foram realizadas com um difratômetro de quatro círculos de geometria Kappa. Os dados cristalinos relevantes são: [Eu (TMU)6] (AsF6)3, TMU = C5H12N2O. Fórmula química: EuC30H72N12O6As3F18; cela unitária é cúbica, a = 18,000 (3)Åe V = 5832 (3)޵ grupo espacial: F23 número - 196 da Internacional Tables For X-Ray Crystallography; número de moléculas por cela unitária: Z = 4; coeficiente de absorção de massa para radiação de molibdênio: µ (MoKα)=27,4 cm-1; densidade calculada: Dc = 1,60 g.cm-3 Para um cristal de tamanho aproximadamente 0,25 x 0,25 x 0,30mm foram medidas 2309 reflexões. A média das intensidades das reflexões equivalentes por simetria de Laue foi calculada obtendo-se um total de 841 independentes, das quais, apenas 277 resultaram maiores que três vezes o desvio padrão estimado de contagem estatística. A estrutura se mostrou altamente desordenada e o modelo proposto refinou a um fator-R final de 13.8%. Os átomos de európio e arsênio estão localizados em posições especiais de simetria pontual local 23 (T) O Eu3+ está hexacoordenado através dos oxigênios das moléculas de TMU formando um octaedro regular de simetria pontual Oh. A distância európio-oxigênio é de aproximadamente 2...

Preliminary X-ray crystallographic studies of a Lys49-phospholipase A 2 homologue from Bothrops pirajai venom complexed with p-bromophenacyl bromide and α-tocopherol inhibitors

Dos Santos, Juliana I.; Marchi-Salvador, Daniela P.; Fernandes, Carlos A.H.; Silveira, Lucas B.; Soares, Andreimar M.; Fontes, Marcos R.M.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 698-701
ENG
Relevância na Pesquisa
700.2356%
PrTX-I, a non-catalytic and myotoxic Lys49-PLA2 from Bothrops pirajai venom has been crystallized alone and in complex with bromophenacyl bromide (BPB), α-tocopherol and α-tocopherol acetate inhibitors. These crystals have shown to diffract X-rays between 2.34 and 1.65 Å resolution. All complexes crystals are isomorphous and belong to the space group P21 whereas native PrTX-I crystals belong to the P3121. © 2007 Bentham Science Publishers Ltd.

Projeto ótico de linha de luz de raios-X duros para cristalografia de proteínas; Optical design of a hard X-ray beamline to protein crystallography

Walan Cesar Grizolli
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 14/07/2010 PT
Relevância na Pesquisa
704.1808%
Diversas áreas da ciência moderna têm sido beneficiadas pelo uso da radiação síncrotron. As técnicas disponveis em laboratórios deste tipo abrangem pesquisas em ciências básicas como Física, Química e Biologia bem como em áreas como engenharia de materiais e farmacologia. Em particular, o uso do espectro de raios-x duros (8-20keV) das fontes síncrotron atuais e crucial para técnicas estruturais como difração e cristalografia. Estas técnicas estão disponíveis para a comunidade científica brasileira no Laboratório Nacional de Luz Síncrotron (LNLS), cujas linhas de luz estão abertas a usuários externos desde 1997. As instalações do LNLS vem sendo constantemente aperfeiçoadas, permitindo o estudo de novos casos científicos como consequência destas melhorias. Neste trabalho estudamos as propriedades da fonte de radiação síncrotron do LNLS e realizamos um projeto óptico para uma linha de luz direcionada a cristalografia de proteínas. Com uso de simulações computacionais propomos opções para melhoria do fluxo de flotons nas linhas de cristalografia de proteínas já existentes. Nossos estudos apontam para a viabilidade de construção de uma linha lateral a linha de luz MX2, utilizando a mesma fonte Wiggler já instalada...

In situ X-ray crystallographic study of the structural evolution of colloidal crystals upon heating

Zozulya, A. V.; Meijer, J.-M.; Shabalin, A.; Ricci, A.; Westermeier, F.; Kurta, R. P.; Lorenz, U.; Singer, A.; Yefanov, O.; Petukhov, A. V.; Sprung, M.; Vartanyants, I. A.
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
780.3985%
The results of a real-time X-ray crystallographic study of the melting transition of polystyrene colloidal crystals during heating are presented.

X-ray characterization of Ge dots epitaxially grown on nanostructured Si islands on silicon-on-insulator substrates

Zaumseil, Peter; Kozlowski, Grzegorz; Yamamoto, Yuji; Schubert, Markus Andreas; Schroeder, Thomas
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
780.3985%
Selective epitaxial growth of Ge on nanostructured Si islands on silicon-on-insulator substrates is investigated by X-ray diffraction and transmission electron microscopy to prove the compliance effect between the materials and the structural perfection, especially under the use of a thin SiGe buffer layer.

Characterization of SiGe thin films using a laboratory X-ray instrument

Ulyanenkova, Tatjana; Myronov, Maksym; Benediktovitch, Andrei; Mikhalychev, Alexander; Halpin, John; Ulyanenkov, Alex
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
784.6572%
This article reports the characterization of thin SiGe/Si(100) epilayers using reciprocal space maps measured by a laboratory X-ray instrument and a high-resolution X-ray diffraction study of partially relaxed SiGe/Si thin films.

Grazing-incidence X-ray diffraction of single GaAs nanowires at locations defined by focused ion beams

Bussone, Genziana; Schott, Rüdiger; Biermanns, Andreas; Davydok, Anton; Reuter, Dirk; Carbone, Gerardina; Schülli, Tobias U.; Wieck, Andreas D.; Pietsch, Ullrich
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
780.3985%
The crystalline structure of single free-standing GaAs nanowires, grown by molecular beam epitaxy on a GaAs substrate at specific positions defined by focused ion beams, and the substrate regions close to the Au-implanted regions are investigated through grazing-incidence X-ray diffraction.

High-quality quartz single crystals for high-energy-resolution inelastic X-ray scattering analyzers

Hönnicke, Marcelo Goncalves; Huang, Xianrong; Cusatis, Cesar; Koditwuakku, Chaminda Nalaka; Cai, Yong Q.
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
780.3985%
High-quality quartz (α-SiO2) crystals are characterized, and their use for inelastic X-ray scattering analyzers is presented and discussed.

Potential use of V-channel Ge(220) monochromators in X-ray metrology and imaging

Korytár, D.; Vagovič, P.; Végsö, K.; Šiffalovič, P.; Dobročka, E.; Jark, W.; Áč, V.; Zápražný, Z.; Ferrari, C.; Cecilia, A.; Hamann, E.; Mikulík, P.; Baumbach, T.; Fiederle, M.; Jergel, M.
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
780.3985%
Several ways of tuning a higher asymmetry factor (>10) in V-channel X-ray monochromators, for metrological and imaging applications, were analysed. A more than sixfold intensity increase for compositionally and thermally tuned cases was achieved.

SFX analysis of non-biological polycrystalline samples

Zhang, Tao; Jin, Shifeng; Gu, Yuanxin; He, Yao; Li, Ming; Li, Yang; Fan, Haifu
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
EN_US
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694.7046%
Serial femtosecond crystallography (SFX) is capable of collecting three-dimensional single-crystal diffraction data using polycrystalline samples. This may dramatically enhance the power of X-ray powder diffraction. In this paper a test has been performed using simulated diffraction patterns. The test sample is a mixture of two zeolites with crystal grain sizes from 100 to 300 nm. X-ray diffraction snapshots by SFX were simulated and processed using the program suite CrystFEL. Identification according to the primitive unit-cell volume determined from individual snapshots was able to separate the whole set of snapshots into two subsets, which correspond to the two zeolites in the sample. Monte Carlo integration in CrystFEL was then applied to them separately. This led to two sets of three-dimensional single-crystal diffraction intensities, based on which crystal structures of the two zeolites were solved easily by direct methods implemented in the program SHELXD.

Live small-animal X-ray lung velocimetry and lung micro-tomography at the Australian Synchrotron Imaging and Medical Beamline

Murrie, R.P.; Morgan, K.S.; Maksimenko, A.; Fouras, A.; Paganin, D.M.; Hall, C.; Siu, K.K.W.; Parsons, D.W.; Donnelley, M.
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
Publicado em //2015 EN
Relevância na Pesquisa
788.4609%
The high flux and coherence produced at long synchrotron beamlines makes them well suited to performing phase-contrast X-ray imaging of the airways and lungs of live small animals. Here, findings of the first live-animal imaging on the Imaging and Medical Beamline (IMBL) at the Australian Synchrotron are reported, demonstrating the feasibility of performing dynamic lung motion measurement and high-resolution micro-tomography. Live anaesthetized mice were imaged using 30 keV monochromatic X-rays at a range of sample-to-detector propagation distances. A frame rate of 100 frames s(-1) allowed lung motion to be determined using X-ray velocimetry. A separate group of humanely killed mice and rats were imaged by computed tomography at high resolution. Images were reconstructed and rendered to demonstrate the capacity for detailed, user-directed display of relevant respiratory anatomy. The ability to perform X-ray velocimetry on live mice at the IMBL was successfully demonstrated. High-quality renderings of the head and lungs visualized both large structures and fine details of the nasal and respiratory anatomy. The effect of sample-to-detector propagation distance on contrast and resolution was also investigated, demonstrating that soft tissue contrast increases...

SAXS and X-ray Crystallography Studies of the Cellulosome from Clostridium thermocellum

Currie, Mark
Fonte: Quens University Publicador: Quens University
Tipo: Tese de Doutorado
EN; EN
Relevância na Pesquisa
695.09484%
Cellulosomes are the most efficient plant cell wall degradation machines discovered to date. All cellulosomal components contain protein modules connected by linkers of varying lengths, which are predicted to be flexible. Consequently, structural studies of the cellulosome have employed a “dissect and build” strategy, whereby individual modules are studied in isolation with the hope to later model the intact complex. However, representative individual structures are now available for all of the cellulosome modules and many questions still remain. The studies described in this thesis depart from the ‘dissection’ stage and enter the ‘build’ stage of cellulosome structural studies of the cellulosome from Clostridium thermocellum. We first describe the crystal structure of a heterodimeric complex comprising the type-II cohesin (CohII) from cell surface anchoring protein SdbA and a trimodular C-terminal truncation of the CipA scaffoldin protein containing the ninth type-I cohesin module (CohI9), a linker, the X-module (X), and the type-II dockerin module (DocII). This structure revealed novel intertwining of scaffoldin molecules and extensive reciprocal contacts between the CohI9 and the X-module of another scaffoldin molecule. Sedimentation velocity experiments indicate dimerization also occurs in solution. We have carried out the crystallization and structural analysis of a heterotrimeric complex containing the CohI9—X-DocII:CohII complex bound to the type-I dockerin module (DocI) from the Cel9D enzyme...

Preliminary X-ray crystallographic studies of a Lys49-phospholipase A(2) homologue from Bothrops pirajai venom complexed with p-bromophenacyl bromide and alpha-tocopherol inhibitors

Santos, Juliana I. dos; Marchi-Salvador, Daniela P.; Fernandes, Carlos A. H.; Silveira, Lucas B.; Soares, Andreimar M.; Fontes, Marcos R. M.
Fonte: Bentham Science Publ Ltd Publicador: Bentham Science Publ Ltd
Tipo: Artigo de Revista Científica Formato: 698-701
ENG
Relevância na Pesquisa
700.2356%
PrTX-I, a non-catalytic and myotoxic Lys49-PLA(2) from Bothrops pirajai venom has been crystallized alone and in complex with bromophenacyl bromide (BPB), alpha-tocopherol and alpha-tocopherol acetate inhibitors. These crystals have shown to diffract X-rays between 2.34 and 1.65 angstrom resolution. All complexes crystals are isomorphous and belong to the space group P2(1) whereas native PrTX-I crystals belong to the P3(1)21.

Deformable complex network for refining low-resolution X-ray structures

Zhang, Chong; Wang, Qinghua; Ma, Jianpeng
Fonte: Universidade Rice Publicador: Universidade Rice
Tipo: Journal article; Text; publisher version
ENG
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789.7869%
In macromolecular X-ray crystallography, building more accurate atomic models based on lower resolution experimental diffraction data remains a great challenge. Previous studies have used a deformable elastic network (DEN) model to aid in low-resolution structural refinement. In this study, the development of a new refinement algorithm called the deformable complex network (DCN) is reported that combines a novel angular network-based restraint with the DEN model in the target function. Testing of DCN on a wide range of low-resolution structures demonstrated that it constantly leads to significantly improved structural models as judged by multiple refinement criteria, thus representing a new effective refinement tool for low-resolution structural determination.

YZGD pyridoxal phosphatase from P. thiaminolyticus; subcloning, expression, and purification for x-ray crystallography structure determination

Strassner, Amanda M.
Fonte: Rochester Instituto de Tecnologia Publicador: Rochester Instituto de Tecnologia
Tipo: Tese de Doutorado
EN_US
Relevância na Pesquisa
689.7869%
The HAD superfamily contains enzymes that catalyze carbon or phosphate transfer reactions. One family within the HAD superfamily is the p-nitrophenyl phosphatase (PNPPase) family. Members of this family are all phosphatases that cleave the substrate analog pnitrophenyl phosphate, in addition to their biologically significant substrate. Interestingly, among the small number of family members that have been characterized, there is a wide variety of biologically relevant activities. YZGD from Paenibacillus thiaminolyticus has pyridoxal phosphatase activity, catalyzed by a HAD superfamily domain, as well as a CDP-alcohol/sugar nucleotide hydrolase activity, catalyzed by a Nudix hydrolase domain. Determining the structure of this enzyme would result in valuable information to contribute to our knowledge and understanding of the HAD superfamily and the Nudix hydrolase superfamily. Sufficient quantities of sufficiently pure YZGD are necessary to perform x-ray crystal structure determination. A histidine tag was added to the enzyme so that it can be purified by nickel affinity chromatography. This was done by cloning the gene into pET19b, a plasmid containing a sequence encoding a string of ~10 histidines, and expressing the protein in Escherichia coli BLR(DE3). The tagged YZGD expresses well...

Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography

McDevitt, Christopher A.; Shintre, Chitra A.; Grossmann, J Gunter; Pollock, Naomi L.; Prince, Stephen; Callaghan, Richard; Ford, Robert C.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
697.7309%
P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure.