Página 1 dos resultados de 799 itens digitais encontrados em 0.002 segundos

Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers; Evaluación microbiológica de las diferentes formulaciones antisépticas, polivinil-pirrolidona-yodo y clorexidina, después de la contaminación intencional de los recipientes; Avaliação microbiológica das diferentes formulações anti-sépticas, polivinilpirrolidona-iodo e clorexidina, após contaminação intencional das almotolias

PADOVANI, Christiane Moreira; GRAZIANO, Kazuko Uchikawa; GOVEIA, Vânia Regina
Fonte: Escola de Enfermagem de Ribeirão Preto / Universidade de São Paulo Publicador: Escola de Enfermagem de Ribeirão Preto / Universidade de São Paulo
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
278.80588%
This study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations - povidone-iodine (PVP-I) and chlorhexidine (CHX) - after intentional contamination, and to establish the minimum care necessary to ensure sterilization of non-disposable antiseptic solution containers. A laboratory study was performed with 180 antiseptic containers, which were contaminated with Serratia marcescens [1 x 105 UFC/mL]. The containers were closed and stored, at room temperature, during seven days and shaken daily. The antiseptic cultures were evaluated to be 100% negative to Serratia marcescens in all of the non-disposable containers. These results suggested that antiseptic solutions inactivate microorganisms [1 x 105 UFC/mL]. Since cleaned antiseptic containers have around 102 UFC coming from tap water, it can be inferred that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of CHX and PVP-I solutions in aqueous, detergent and alcoholic formulations.; Los objetivos de este estudio fueron evaluar la sobre vivencia de los micro organismos en las diferentes formulaciones de los antisépticos clorexidina (CHX) y polivinil-pirrolidona-yodo (PVP-Y), después de una contaminación intencional de los recipientes y extrapolar los resultados de los laboratorios para el cuidado mínimo a ser dispensado a los recipientes de múltiple uso para el envase de los antisépticos probados. Para esto...

Avaliação microbiológica das diferentes formulações anti-sépticas-polivilipirrolidona-iodo e clorexidina - após contaminação intencional das almotolias; Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of the solution recipients

Padovani, Christiane Moreira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 22/04/2008 PT
Relevância na Pesquisa
384.51414%
Introdução: Há relatos na literatura sobre anti-sépticos que sofreram contaminação, reportados como causas de surtos de infecção em estabelecimentos de assistência à saúde. Em relação aos recipientes que veiculam essas soluções, a preferência por almotolias descartáveis, de pequeno volume, que possibilitem a individualização do uso e o consumo rápido do produto é largamente difundida. No entanto, constata-se que a utilização de almotolias coletivas e reprocessáveis, tem sido a prática mais comum nas instituições de saúde do país. A forma de processamento de almotolias para envase de anti-sépticos não está esclarecida na literatura e é amplamente discutida nos serviços de saúde sem respaldo científico. Objetivos: os objetivos deste estudo constituiram-se em avaliar a sobrevivência dos microrganismos nas diferentes formulações dos anti-sépticos - CHX e PVPI-I - após contaminação intencional das almotolias com Serratia marcescens e extrapolar os resultados laboratoriais para o cuidado mínimo a ser dispensado às almotolias de múltiplo uso para o envase dos anti-sépticos testados. Método: Foi conduzido um estudo laboratorial, em que 180 almotolias foram contaminadas com 1 x 105 UFC/mL de suspensão contendo S. marcescens. Nas almotolias contaminadas...

Design of antiseptic formulations containing extract of Plinia cauliflora

Oliveira, Lara Alexandre de; Souza-Moreira, Tatiana Maria de; Cefali, Letícia Caramori; Chiari, Bruna Galdorfini; Corrêa, Marcos Antonio; Isaac, Vera Lucia Borges; Salgado, Hérida Regina Nunes; Pietro, Rosemeire Cristina Linhari Rodrigues
Fonte: Universidade de São Paulo (USP), Faculdade de Ciências Farmacêuticas Publicador: Universidade de São Paulo (USP), Faculdade de Ciências Farmacêuticas
Tipo: Artigo de Revista Científica Formato: 525-533
ENG
Relevância na Pesquisa
374.44793%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); As folhas da espécie brasileira Plinia cauliflora foram utilizadas a fim de se obter um extrato hidroalcoólico e frações ativas proporcionando o desenvolvimento de eficazes formulações farmacêuticas antissépticas. A composição química do extrato etanólico 70%, fração aquosa e acetato de etila foi analisada por cromatografia em camada delgada e teor de fenóis. A atividade antimicrobiana foi testada frente a Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus acidophilus e Candida albicans por difusão em ágar e a concentração inibitória mínima foi determinada por microdiluição. A qualidade microbiológica do extrato foi avaliada para evitar a contaminação das formulações. Foram desenvolvidos um enxaguatório bucal e um creme tópico contendo o extrato sendo que a atividade antisséptica foi ensaiada por difusão em ágar. A estabilidade sensorial e físico-química foram testadas. A cromatografia indicou a presença de terpenos, flavonóides e taninos no extrato e frações, observando-se alto teor de fenóis totais. As amostras vegetais foram ativas contra todos os micro-organismos testados, exceto Lactobacillus acidophilus. Ambas as formulações apresentaram atividade antisséptica e estabilidade. Desta forma...

Avaliação da atividade anti-séptica de extrato seco de Stryphnodendron adstringens (Mart.) Coville e de preparação cosmética contendo este extrato

Souza, Tatiana M.; Moreira, Raquel R.D.; Pietro, Rosemeire C.L.R.; Isaac, Vera L.B.
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: 71-75
POR
Relevância na Pesquisa
265.9396%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); As espécies pertencentes ao gênero Stryphnodendron são conhecidas como barbatimão e são nativas do cerrado brasileiro. Possuem taninos como metabólitos secundários primordiais de suas cascas apresentando, entre outras, atividade antimicrobiana e cicatrizante. A concentração bactericida mínima (CBM) do extrato seco das cascas de Stryphnodendron adstringens frente a duas bactérias Gram-positivas e uma bactéria Gram-negativa foi determinada pela técnica de diluição em tubos. A atividade antimicrobiana do extrato seco e a atividade anti-séptica de sabonete líquido contendo o extrato seco foram avaliadas pelo método de difusão em ágar. O extrato apresentou valores de concentração bactericida mínima de 50 mg/mL frente a Staphylococcus aureus e 75 mg/mL contra Staphylococcus epidermidis e Escherichia coli e no teste de difusão em ágar, S. aureus apresentou maior sensibilidade ao extrato seco que as outras bactérias. O sabonete líquido mostrou maior eficiência na atividade anti-séptica contra as bactérias testadas na concentração de 100 mg de extrato/mL de sabonete.; The species of the genus Stryphnodendron are known as barbatimão and are native from Brazilian cerrado. They present tannins as main secondary metabolites of their barks and show antimicrobial and cicatrisation activity among others. The minimum bactericidal concentration (MBC) of the dry extract from the barks of Stryphnodendron adstringens against two Gram-positive and one Gram-negative bacteria was done using methodology of broth dilution in tubes. The antimicrobial activity of the dry extract and the antiseptic activity of the liquid soap containing that dry extract were evaluated by agar difusion method. For the extract were obtained the minimum bactericidal concentration values of 50 mg/mL against Staphylococcus aureus and 75 mg/mL against Staphylococcus epidermidis and Escherichia coli and by agar difusion...

Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers

Padovani,Christiane Moreira; Graziano,Kazuko Uchikawa; Goveia,Vânia Regina
Fonte: Escola de Enfermagem de Ribeirão Preto / Universidade de São Paulo Publicador: Escola de Enfermagem de Ribeirão Preto / Universidade de São Paulo
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2008 EN
Relevância na Pesquisa
278.80588%
This study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations - povidone-iodine (PVP-I) and chlorhexidine (CHX) - after intentional contamination, and to establish the minimum care necessary to ensure sterilization of non-disposable antiseptic solution containers. A laboratory study was performed with 180 antiseptic containers, which were contaminated with Serratia marcescens [1 x 105 UFC/mL]. The containers were closed and stored, at room temperature, during seven days and shaken daily. The antiseptic cultures were evaluated to be 100% negative to Serratia marcescens in all of the non-disposable containers. These results suggested that antiseptic solutions inactivate microorganisms [1 x 105 UFC/mL]. Since cleaned antiseptic containers have around 102 UFC coming from tap water, it can be inferred that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of CHX and PVP-I solutions in aqueous, detergent and alcoholic formulations.

In vitro activity of antifungal and antiseptic agents against dermatophyte isolates from patients with tinea pedis

Soares,Maria Magali Stelato Rocha; Cury,Arlete Emily
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2001 EN
Relevância na Pesquisa
371.01188%
The in vitro activity of antifungal and antiseptic agents were evaluated against dermatophytes isolated from patients with tinea pedis. The antifungals studied were: ciclopirox olamine, cetoconazole, tolciclate and terbinafine, and the antiseptics were: povidine iodine (PVPI), propolis, Fungol®, Andriodermol®, and boric acid. The minimum inhibitory concentration (MIC) or the minimal dilution concentration (MDC) was determined by an agar dilution method using modified yeast nitrogen agar base, and the minimum fungicidal concentration (MFC) or minimum fungicidal dilution (MFD) was determined with subcultures on Sabouraud dextrose agar. All drugs studied were active against the dermatophytes at lower concentrations than those used in products and/or pharmaceutical preparations for topical use. Some antifungal agents, mainly terbinafine and tolciclate, presented higher efficacy than the other drugs, with lower MICs and MFCs values. It was concluded that the use of these antiseptic drugs represent an excellent alternative for the topical treatment of tinea pedis. For the treatment of severe cases these are the antifungal agents of choice.

Design of antiseptic formulations containing extract of Plinia cauliflora

Oliveira,Lara Alexandre de; Souza-Moreira,Tatiana Maria de; Cefali,Letícia Caramori; Chiari,Bruna Galdorfini; Corrêa,Marcos Antonio; Isaac,Vera Lucia Borges; Salgado,Hérida Regina Nunes; Pietro,Rosemeire Cristina Linhari Rodrigues
Fonte: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Publicador: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2011 EN
Relevância na Pesquisa
376.92953%
The leaves of the Brazilian species Plinia cauliflora were used to obtain active hydroalcoholic extract and fractions enabling the development of efficient antiseptic pharmaceutical formulations. A chemical composition of 70% ethanol extract, aqueous and ethyl acetate fractions was analyzed by thin-layer chromatography and for phenol content. Antimicrobial activity was tested against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus acidophilus and Candida albicans by the agar diffusion method and the minimum inhibitory concentration was assayed by broth microdilution. Extract microbiological quality was tested to avoid contamination in the formulations. A mouthwash and a topical cream containing the extract were developed and antiseptic activity was assessed by agar diffusion. Sensory and physicochemical stability of the formulations were assayed. Chromatography indicated the presence of terpenes, flavonoids and tannins in the extract and fractions and total phenol content were found to be high. The plant samples were active against all the microorganisms tested, except for Lactobacillus acidophilus. Both topical formulations showed antiseptic activity and stability. Thus, these may be used as antimicrobials in skin infections...

Comparison of Four Antiseptic Preparations for Skin in the Prevention of Contamination of Percutaneously Drawn Blood Cultures: a Randomized Trial

Calfee, David P.; Farr, Barry M.
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
Publicado em /05/2002 EN
Relevância na Pesquisa
265.9396%
A number of skin antiseptics have been used to prevent the contamination of blood cultures, but the comparative efficacies of these agents have not been extensively evaluated. We therefore sought to compare the efficacy of four skin antiseptics in preventing blood culture contamination in a randomized, crossover, investigator-blinded study conducted in an emergency department and the inpatient wards of a university hospital. The patient group included all patients from whom blood samples were obtained percutaneously for culture. Skin antisepsis was performed with 10% povidone-iodine, 70% isopropyl alcohol, tincture of iodine, or povidone-iodine with 70% ethyl alcohol (i.e., Persist). The blood culture contamination rate associated with each antiseptic was then determined. A total of 333 (2.62%) of 12,692 blood cultures were contaminated during the study period compared to 413 (3.21%) of 12,859 blood cultures obtained during the previous 12-month period (relative risk = 0.82; 95% confidence interval, 0.71 to 0.94; P = 0.006). During the study, the contamination rates were determined to be 2.93% with povidone-iodine, 2.58% with tincture of iodine, 2.50% with isopropyl alcohol, and 2.46% with Persist (P = 0.62). We detected no significant differences in the blood culture contamination rates among these four antiseptics...

Effectiveness of Septisol Antiseptic Foam as a Surgical Scrub Agent

Dewar, Norman E.; Gravens, Daniel L.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /10/1973 EN
Relevância na Pesquisa
271.0119%
Septisol antiseptic foam (0.23% hexachlorophene in a 46% ethyl alcohol base) is a new surgical scrub agent for both primary and re-entry use that is designed to minimize the harsh effects to the skin of the conventional scrub while retaining effective antibacterial properties. A preliminary surgical scrub study of 1-week duration yielded an immediate reduction in resident flora of 92% from an average single scrub coupled with a residual bacteriostatic effect from repeated use that gave a plateau at 57% of the pretest resident population level. A separate study demonstrated complete elimination of both gram-positive and gram-negative transients from the skin with a single application of the product. In an 8-week surgical scrub study, equal effectiveness was shown between Septisol antiseptic foam and a standard 3% hexachlorophene detergent. However, Septisol antiseptic foam offers considerable advantage in minimizing the harsh effects to the skin of the conventional surgical scrub and results in a substantially lower hemic level of hexachlorophene in the user than that obtained with 3% hexachlorophene detergent. Sampling was conducted by the fingerprint impression plate technique of Gale.

Antimicrobial Agent of Susceptibilities and Antiseptic Resistance Gene Distribution among Methicillin-Resistant Staphylococcus aureus Isolates from Patients with Impetigo and Staphylococcal Scalded Skin Syndrome

Noguchi, Norihisa; Nakaminami, Hidemasa; Nishijima, Setsuko; Kurokawa, Ichiro; So, Hiromu; Sasatsu, Masanori
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /06/2006 EN
Relevância na Pesquisa
271.0119%
The susceptibilities to antimicrobial agents of and distributions of antiseptic resistance genes in methicillin-resistant Staphylococcus aureus (MRSA) strains isolated between 1999 and 2004 in Japan were examined. The data of MRSA strains that are causative agents of impetigo and staphylococcal scalded skin syndrome (SSSS) were compared with those of MRSA strains isolated from patients with other diseases. The susceptibilities to antiseptic agents in MRSA isolates from patients with impetigo and SSSS were higher than those in MRSA isolates from patients with other diseases. The distribution of the qacA/B genes in MRSA strains isolated from patients with impetigo and SSSS (1.3%, 1/76) was remarkably lower than that in MRSA strains isolated from patients with other diseases (45.9%, 95/207). Epidemiologic typings of staphylococcal cassette chromosome mec (SCCmec) and pulsed-field gel electrophoresis (PFGE) showed that MRSA strains isolated from patients with impetigo and SSSS had type IV SCCmec (75/76), except for one strain, and 64.5% (49/76) of the strains had different PFGE types. In addition, the patterns of restriction digestion of all tested qacA/B plasmid in MRSA isolates having different PFGE types were identical. The results showed that a specific MRSA clone carrying qacA/B was not prevalent...

A STUDY OF THE ANTISEPTIC PROPERTIES OF CERTAIN ORGANIC COMPOUNDS

Kligler, I. J.
Fonte: The Rockefeller University Press Publicador: The Rockefeller University Press
Tipo: Artigo de Revista Científica
Publicado em 01/04/1918 EN
Relevância na Pesquisa
274.44795%
This study of the inhibitive effect of aniline and some of its derivatives and of the triphenylmethane dyes on certain bacteria warrants the following tentative conclusions: 1. The composition and reaction of the medium exert a marked influence on the behavior of the antiseptic. The higher the concentration of organic nitrogenous compounds (peptone) in the medium, the lower is the effective concentration of the dye. The reaction of the medium modifies the specific action of the antiseptic, owing probably to an alteration in the bacterial cell. 2. The germicidal action of the compounds is a function of the benzene nucleus, the added elements or radicals, their number, and, in the case of the dyes, probably the quinoid structure of the nucleus. 3. As far as tested, the increase in the number of alkyl radicals increases the antiseptic power. Methyl green is an interesting exception to this rule, for the change of one of the nitrogens to the quaternary salt is accompanied by an almost complete loss in inhibitive action. 4. The antiseptic power is enhanced to a greater extent by an ethyl than a methyl group, and the second alkyl produces a proportionately greater increase than the first. It appears that the relative position of the introduced group may be a factor in determining the relative improvement in the effectiveness of the compound. 5. The introduction of a methyl group in the nucleus consistently enhances the inhibitive action of the compound and its alkyl derivatives. This is evident from a comparison of the action of aniline and its derivatives with that of toluidine and its corresponding derivatives. 6. The simple aniline derivatives...

Antiseptic technology: access, affordability, and acceptance.

Boyce, J. M.
Fonte: Centers for Disease Control Publicador: Centers for Disease Control
Tipo: Artigo de Revista Científica
Publicado em //2001 EN
Relevância na Pesquisa
274.44795%
Factors other than antimicrobial activity of soaps and antiseptic agents used for hand hygiene by health personnel play a role in compliance with recommendations. Hand hygiene products differ considerably in acceptance by hospital personnel. If switching from a nonmedicated soap to an antiseptic agent or increased use of an existing antiseptic agent for hand hygiene prevented a few more infections per year, additional expenditures for antiseptic agents would be offset by cost savings.

Antiseptic-Impregnated Central Venous Catheters: Their Evaluation in Burn Patients

Ramos, G.; Bolgiani, A.; Patiño, O.; Prezzavento, G.; Guastavino, P.; Durlach, R.; Fernandez Caniggia, L.; Benaim, F.
Fonte: Mediterranean Council for Burns and Fire Disasters Publicador: Mediterranean Council for Burns and Fire Disasters
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
278.80588%
Central venous catheter-related infections are an important source of morbidity and mortality in burn patients. Antiseptic impregnated catheters have been recommended to prevent infections related to central venous lines in high-risk patients who require short-term catheters. This prospective, randomized, and controlled study compared the efficacy of standard and antiseptic devices in reducing catheter-related infections in burn patients. Twenty-two patients were included in the study with an average age of 47.6 yr and an average burned total body surface area of 38.7%. Thirty-eight silver-sulphadiazine, chlorhexidine catheters were compared with 40 non-antiseptic catheters. No differences in bacteraemia or colonization rates were observed between standard and antiseptic-coated catheters. Antiseptic catheters were more effective in reducing S. epidermidiscolonization than standard catheters (4% vs 31%, p < 0.01). However, Gram-negative bacilli were responsible more often than Gram-positive cocci for catheter tip colonization (53% vs 46%) and they were responsible for all the bacteraemias (5.1%) related to catheters in the present study. We conclude that antiseptic-impregnated catheters could be more effective for Gram-positive cocci and could therefore be less effective in patients with high Gram-negative bacilli bloodstream infection prevalence...

Synthesis and characterization of antiseptic-based poly(anhydride-esters)

Schmeltzer, Robert C.; Uhrich, Kathryn E.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 01/07/2006 EN
Relevância na Pesquisa
271.0119%
Poly(anhydride-esters) were prepared from catechol, fenticlor and hexachlorophene. The molecular weights (Mw) of the polymers were typically > 10,000 Da with glass transition temperatures (Tg) ranging from 23 to 84 °C. The thermal characteristics of the polymers paralleled the melting temperatures of the chemically incorporated antiseptic molecules. The in vitro release of the chemically incorporated antiseptic molecules were monitored over a 12 week period. For comparison, the in vitro release of physically admixed antiseptic molecules were also observed. After 12 weeks, the polymers were not completely degraded with drug release ranging from less than 1 to 55 %. Sessile-drop contact angles indicated that the polymers were relatively hydrophobic, contributing to the slow polymer degradation rates.

The role of antiseptic agents in atopic dermatitis

Lee, Melissa; Van Bever, Hugo
Fonte: Asia Pacific Association of Allergy, Asthma and Clinical Immunology Publicador: Asia Pacific Association of Allergy, Asthma and Clinical Immunology
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
280.27432%
The skin of individuals with atopic dermatitis has a susceptibility to be colonized with Staphylococcus aureus. This has been associated with increased frequency and severity of exacerbations of atopic dermatitis. Therefore, there is a growing interest in the use of antiseptic agents to target primary bacterial colonization and infection. Antiseptic agents have been found to be better tolerated and less likely to induce bacterial resistance as compared to antibiotics. There is also a wide variety of antiseptic agents available. The efficacy of antiseptic agents has yet to be established as the studies reviewed previously have been small and of suboptimal quality. This review discusses the rationale behind targeting S. aureus with antiseptic agents and presents findings from a review of studies assessing the efficacy of antiseptics in atopic dermatitis in the last five years. Four studies were found, including a bleach bath study which has already been reviewed elsewhere. The remaining 3 studies assessed the efficacy of sodium hypochlorite containing cleansing body wash, sodium hypochlorite baths and 1% triclosan in leave on emollient. These studies suggested some benefit for the inclusion of antiseptic use with the mainstay management of atopic dermatitis...

Class 1 Integrons and the Antiseptic Resistance Gene (qacEΔ1) in Municipal and Swine Slaughterhouse Wastewater Treatment Plants and Wastewater—Associated Methicillin-Resistant Staphylococcus aureus

Wan, Min Tao; Chou, Chin Cheng
Fonte: MDPI Publicador: MDPI
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
276.92955%
Class 1 integrons are mobile gene elements (MGEs) containing qacEΔ1 that are resistant to quaternary ammonium compound (QAC) disinfectants. This study compared the abundances of class 1 integrons and antiseptic resistance genes in municipal (M) and swine slaughterhouse (S) wastewater treatment plants (WWTPs) and investigated the presence of class 1 integrons and antiseptic resistance genes in methicillin-resistant Staphylococcus aureus (MRSA) isolated from wastewater samples. The abundances of intI1 and qacEΔ1 genes in 96 wastewater samples were quantified using real-time quantitative polymerase chain reaction (real-time qPCR), and 113 MRSA isolates recovered from the wastewater samples were detected class 1 integrons and linked antiseptic resistance genes (qacEΔ1), and minimum inhibitory concentrations (MICs) for QAC antiseptics. The intI1 and qacEΔ1 genes were detected in all the wastewater samples, and they were more abundant in S-WWTP samples than in M-WWTP samples. A higher percentage of MRSA isolates carried qacEΔ1 in MRSA from swine wastewater samples (62.8%) than in municipal MRSA (3.7%). All the MRSA isolates showed high MICs for antiseptic agents. This study provides important evidence regarding the abundances of intI1 and qacEΔ1 genes in municipal and swine slaughterhouse wastewater...

Demonstrating that chlorine dioxide is a size-selective antimicrobial agent and high purity ClO2 can be used as a local antiseptic

Noszticzius, Zoltán; Wittmann, Maria; Kály-Kullai, Kristóf; Beregvári, Zoltán; Kiss, István; Rosivall, László; Szegedi, János
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 18/04/2013
Relevância na Pesquisa
271.0119%
Background / Aims ClO2, the so-called "ideal biocide", could also be applied as an antiseptic if it was understood why the solution's rapid killing of microbes does not cause any harm to humans or to animals. Our aim was to study both theoretically and experimentally its reaction-diffusion mechanism to find the source of that selectivity. Methods ClO2 permeation measurements through protein membranes were performed and the time delay of ClO2 transport due to reaction and diffusion was determined. To calculate ClO2 penetration depths and estimate bacterial killing times, approximate solutions of the reaction-diffusion equation were derived. Additionally, as a preliminary test, three patients with infected wounds were treated with a 300 ppm high purity ClO2 solution and the healing process was documented. Results The rate law of the reaction-diffusion model predicts that the killing time is proportional to the square of the characteristic size (e.g. diameter) of a body, thus, small ones will be killed extremely fast. For example, the killing time for a bacterium is on the order of milliseconds in a 300 ppm ClO2 solution. Thus, the few minutes of contact time (owing to the volatility of ClO2) is quite enough to kill all bacteria, but short enough to keep ClO2 penetration into the living tissues safely below 0.1 mm...

ESTUDO DA EFICÁCIA DE UM NOVO PRODUTO À BASE DE ÁLCOOL GEL UTILIZADO NA ANTI-SEPSIA EM UM SERVIÇO DE NEFROLOGIA; STUDY OF THE EFFECTIVENESS OF A NEW PRODUCT TO THE ALCOHOL GEL BASE UTILIZED AS AN ANTISEPTIC IN A NEPHROLOGY SERVICE

Burg, Geni; Portela, Odete; Paraginski, Gustavo Luiz; Souza, Viviane; Silveira, Djalma Dias; Hörner, Rosmari
Fonte: Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto Publicador: Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Formato: application/pdf
Publicado em 30/06/2007 POR
Relevância na Pesquisa
271.0119%
A atenção à saúde é permanentemente desafiada pelas infecções relacionadas aos procedimentos assistenciais, que resultam no aumento da morbidade, mortalidade e custos operacionais. O álcool etílico possui propriedades antimicrobianas reconhecidas, capazes de eliminar os microrganismos mais freqüentemente envolvidos nas infecções em serviços de saúde. A maior desvantagem do álcool para a anti-sepsia da pele é seu efeito ressecante. Este estudo foi realizado na Clínica Renal de Santa Maria, RS, com o objetivo de avaliar a eficácia antiséptica de um novo produto à base de álcool gel (Biogel®), na tentativa de eliminar a necessidade da lavagem com água e sabão do braço com a Fístula Artério Venosa (FAV), dos pacientes submetidos à hemodiálise. A metodologia utilizada foi um estudo experimental do tipo “Ensaio Clínico Randomizado” realizado com onze pacientes ambulatoriais submetidos à hemodiálise nos meses de janeiro a fevereiro de 2005. Foi efetuada cultura da pele anterior e posterior ao procedimento da anti-sepsia, utilizando o álcool 70 % e o produto Biogel®, e avaliação da atividade antibacteriana do Biogel®, frente à bactérias gram-positivas e gram-negativas correspondentes à cepas nosocomiais isoladas de pacientes atendidos no Hospital Universitário de Santa Maria (HUSM). Os resultados mostraram igual desempenho dos dois métodos de anti-sepsia...

Design of antiseptic formulations containing extract of Plinia cauliflora

Oliveira, Lara Alexandre de; Souza-Moreira, Tatiana Maria de; Cefali, Letícia Caramori; Chiari, Bruna Galdorfini; Corrêa, Marcos Antonio; Isaac, Vera Lucia Borges; Salgado, Hérida Regina Nunes; Pietro, Rosemeire Cristina Linhari Rodrigues
Fonte: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas Publicador: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; ; Formato: application/pdf
Publicado em 01/09/2011 ENG
Relevância na Pesquisa
374.44793%
As folhas da espécie brasileira Plinia cauliflora foram utilizadas a fim de se obter um extrato hidroalcoólico e frações ativas proporcionando o desenvolvimento de eficazes formulações farmacêuticas antissépticas. A composição química do extrato etanólico 70%, fração aquosa e acetato de etila foi analisada por cromatografia em camada delgada e teor de fenóis. A atividade antimicrobiana foi testada frente a Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus acidophilus e Candida albicans por difusão em ágar e a concentração inibitória mínima foi determinada por microdiluição. A qualidade microbiológica do extrato foi avaliada para evitar a contaminação das formulações. Foram desenvolvidos um enxaguatório bucal e um creme tópico contendo o extrato sendo que a atividade antisséptica foi ensaiada por difusão em ágar. A estabilidade sensorial e físico-química foram testadas. A cromatografia indicou a presença de terpenos, flavonóides e taninos no extrato e frações, observando-se alto teor de fenóis totais. As amostras vegetais foram ativas contra todos os micro-organismos testados, exceto Lactobacillus acidophilus. Ambas as formulações apresentaram atividade antisséptica e estabilidade. Desta forma...

Avaliação microbiológica das diferentes formulações anti-sépticas, polivinilpirrolidona-iodo e clorexidina, após contaminação intencional das almotolias; Evaluación microbiológica de las diferentes formulaciones antisépticas, polivinil-pirrolidona-yodo y clorexidina, después de la contaminación intencional de los recipientes; Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers

Padovani, Christiane Moreira; Graziano, Kazuko Uchikawa; Goveia, Vânia Regina
Fonte: Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto Publicador: Universidade de São Paulo. Escola de Enfermagem de Ribeirão Preto
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Formato: application/pdf; application/pdf; application/pdf
Publicado em 01/12/2008 ENG; POR; SPA
Relevância na Pesquisa
278.80588%
Os objetivos deste estudo foram: avaliação da sobrevivência dos microrganismos nas diferentes formulações dos anti-sépticos clorexidina (CHX) e polivinilpirrolidona-iodo (PVP-I), após contaminação intencional das almotolias, e extrapolar os resultados laboratoriais para o cuidado mínimo a ser dispensado às almotolias de múltiplo uso para o envase dos anti-sépticos testados. Para tanto, foi desenvolvido estudo laboratorial, em que 180 almotolias foram contaminadas com 1 x 105 UFC/mL de suspensão, contendo S.marcescens. Após a contaminação, seis diferentes formulações de anti-sépticos (clorexidina e PVP-I nos veículos alcoólico, degermante e aquoso) foram distribuídas e submetidas à cultura diária durante sete dias, a fim de verificar se houve crescimento do microrganismo. Os resultados desta investigação permitem a recomendação de que a limpeza como procedimento mínimo no processamento desses recipientes garante a segurança de sua utilização repetida para distribuição dos anti-sépticos testados - CHX e PVP-I.; Los objetivos de este estudio fueron evaluar la sobre vivencia de los micro organismos en las diferentes formulaciones de los antisépticos clorexidina (CHX) y polivinil-pirrolidona-yodo (PVP-Y)...