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Enzymatic Solid-Phase Reactor Based on Silica Organofunctionalized with p-Phenylenediamine for Electrochemical Detection of Phenolic Compounds

Moraes, Fernando C.; Cesarino, Ivana; Golinelli, Diego L. C.; Machado, Sergio Antonio Spinola
Fonte: AMER SCIENTIFIC PUBLISHERS; VALENCIA Publicador: AMER SCIENTIFIC PUBLISHERS; VALENCIA
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
36.769878%
A new biomaterial, based on silica organofunctionalized with p-phenylenediamine (p-PDA) and the enzyme peroxidase, was used in the development of an enzymatic solid-phase reactor. The analytical techniques used in the characterization showed that the organic ligand was incorporated into the silica matrix. Thus, the silica modified with p-PDA allowed the incorporation of peroxidase by the electrostatic interaction between the carboxylic groups present in the enzyme molecules and the amino groups attached to the silica. The enzymatic solid-phase reactor was used for chemical oxidation of phenols in 1, 4-benzoquinone that was then detected by chronoamperometry. The system allowed the analysis of hydroquinone with a detection limit of 83.6 nmol L-1. Thus, the new material has potential in the determination of phenolic compounds river water samples.; FAPESP; FAPESP [2010/11049-2, 2010/11567-3]