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Development and validation of a HPLC method for standardization of herbal and commercial extracts of Myrcia uniflora

Batista, Andrea N. de L.; Colombo, Renata; Pascoli, Inara C. de; Teles, Helder L.; Silva, Geraldo H.; Bomfim, Giovanni C. C.; Burgos, Rosilene C. R.; Cavalheiro, Alberto José; Bolzani, Vanderlan da Silva; Silva, Dulce H. S.; Reimberg, Maria Célia H.
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: 402-406
ENG
Relevância na Pesquisa
66.96%
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Myrcia uniflora Barb. Rodr., Myrtaceae, popularly known as pedra-hume-caá in Brazil, is sold as dry extracts in capsules or as tinctures for the treatment of diabetes mellitus. Previous phytochemical studies on this species described the occurrence of the flavonoids mearnsitrin and myricitrin. In the present study, the chromatographic profiles of M. uniflora leaves and commercial extracts were determined using HPLC-PAD. Myricitrin was used as an external standard in the development and validation of the HPLC method. The proposed method is simple, rapid and reliable and can be successfully applied in industry for standardization of herbs and phytomedicines commercialised in Brazil as pedra-hume-caá.

Development and validation of a HPLC method for standardization of herbal and commercial extracts of Myrcia uniflora

Batista,Andrea N. de L.; Colombo,Renata; Pascoli,Inara C. de; Teles,Helder L.; Silva,Geraldo H.; Bomfim,Giovanni C. C.; Burgos,Rosilene C. R.; Cavalheiro,Alberto J.; Bolzani,Vanderlan da Silva; Silva,Dulce H. S.; Reimberg,Maria Célia H.
Fonte: Sociedade Brasileira de Farmacognosia Publicador: Sociedade Brasileira de Farmacognosia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2011 EN
Relevância na Pesquisa
66.96%
Myrcia uniflora Barb. Rodr., Myrtaceae, popularly known as "pedra-hume-caá" in Brazil, is sold as dry extracts in capsules or as tinctures for the treatment of diabetes mellitus. Previous phytochemical studies on this species described the occurrence of the flavonoids mearnsitrin and myricitrin. In the present study, the chromatographic profiles of M. uniflora leaves and commercial extracts were determined using HPLC-PAD. Myricitrin was used as an external standard in the development and validation of the HPLC method. The proposed method is simple, rapid and reliable and can be successfully applied in industry for standardization of herbs and phytomedicines commercialised in Brazil as "pedra-hume-caá".