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A perceção dos profissionais das Clínicas Pedagógicas da Universidade Fernando Pessoa e dos familiares dos indivíduos com Perturbações do Espetro do Autismo (PEA) sobre o modelo TEACCH como estratégia

Duarte, Vera Regina Rodrigues
Fonte: [s.n.] Publicador: [s.n.]
Tipo: Dissertação de Mestrado
Publicado em //2013 POR
Relevância na Pesquisa
36.93%
Dissertação apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências da Educação: Educação Especial, área de especialização em Domínio Cognitivo e Motor; Com o presente estudo pretende-se analisar a perceção de eficácia do modelo Treatment and Education of Autistic and related Communication Handicapped Children (TEACCH) como estratégia de inclusão para indivíduos com Perturbações do Espetro do Autismo (PEA). Deste modo, realizou-se uma revisão bibliográfica, para enquadrar teoricamente a temática, procedendo-se à apresentação do estudo empírico. Esta investigação, de natureza quantitativa, foi desenvolvida com os profissionais das Clínicas Pedagógicas de Psicologia, de Reabilitação Psicomotora e de Terapêutica da Fala da Universidade Fernando Pessoa do Porto, assim como com os familiares dos indivíduos com Perturbações do Espectro do Autismo (PEA) acompanhados nas referidas clínicas. Para a recolha de dados utilizou-se a técnica de inquérito por questionário, através da aplicação de dois questionários distintos, um aos profissionais e outro aos familiares anteriormente mencionados. Embora tenha existido um número significativo de abstenções às questões sobre o modelo TEACCH...

Efeitos da velocidade de rebaixamento do nível freático na produtividade da soja (Glycine max (L.) Merrill) e da ervilha (Pisum sativum L.).; Effects of the drawdown velocity of the water table in the crop yield of soybean (glycime max l. merrill) and of pea (pisum sativum l.).

Sá, Jocelito Saccol de
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 23/01/2002 PT
Relevância na Pesquisa
36.99%
No Brasil, os solos hidromórficos são basicamente utilizados no cultivo de arroz, principalmente na região Sul do Brasil. O plantio de outras culturas em substituição ao arroz, pode ser uma alternativa viável para a melhor exploração destes solos durante o inverno e no pousio da área. O presente trabalho foi realizado em casa de vegetação do Departamento de Engenharia Rural da Escola Superior de Agricultura "Luiz de Queiroz" em Piracicaba, SP, tendo como objetivos avaliar os efeitos do rebaixamento do nível freático no desenvolvimento e na produtividade de plantas de soja, cv. BR-16 e de ervilha, cv. Axé, pelos parâmetros produtivos: altura e massa seca da parte aérea da planta, número de vagens e de grãos produzidos e peso total de grãos por planta; determinar o estádio de desenvolvimento das culturas mais sensível ao excesso de umidade no solo e determinar a velocidade de rebaixamento do nível freático mais adequada ao desenvolvimento das plantas de soja e de ervilha, dentro das condições experimentais propostas. As plantas foram conduzidas em lisímetros construídos em tubos de concreto preenchidos com Latossolo Vermelho-Amarelo Álico. Os ensaios foram realizados nos estádios vegetativo e reprodutivo das culturas...

Amido resistente obtido a partir de amido de leguminosas e de seus hidrolisados; Resistant starch from legumes starches and their hydrolysates

Polesi, Luis Fernando
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 28/08/2009 PT
Relevância na Pesquisa
27.26%
O amido resistente (AR) é a fração do amido que não sofre a ação das enzimas digestivas, apresentando comportamento semelhante ao da fibra dietética. O objetivo do presente trabalho foi avaliar o teor e as características dos AR obtidos a partir dos amidos de ervilha e de grão-de-bico por diferentes processos de redução de massa molecular. Os amidos naturais ou gelatinizados foram submetidos a processos de hidrólise ácida (HCl 2 M por 2,5 h) ou enzimática (pululanase, 40 U/g por 10 h) previamente a um processo controle, que constou de tratamento hidrotérmico (autoclavagem a 121 °C por 30 min), refrigeração (4 °C por 24 h) e liofilização. O material produzido foi caracterizado quanto ao aspecto geral por microscopia eletrônica de varredura (MEV), teor de AR, teor de fibra dietética total (FDT), índice de absorção de água (IAA), índice de solubilidade em água (ISA), padrão de cristalinidade (difração de raios X), viscosidade (RVA - Rapid Visco Analyser) e propriedades térmicas (DSC - Differential Scanning Calorimeter). O teor de AR para o amido natural de ervilha e grãode- bico foi de 39,8 e 31,9 %, respectivamente. Nos amidos processados esse teor variou de 38,5 a 54,6 % para a ervilha e de 16,4 a 32...

Teores de taninos e produção de gases in vitro da silagem de sorgo com adição de níveis crescentes de guandu; Tannins Levels and gas production in vitro of sorghum silage with the addition of increasing levels of pigeon pea

Pinedo, Lerner Arévalo
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 30/11/2009 PT
Relevância na Pesquisa
37.22%
Na alimentação animal, o sorgo pode ser explorado de diversas formas. Além dos grãos, a planta pode ser oferecida na forma de silagem, rolão, verde ou ainda pastejada; embora o valor nutritivo pode sofrer algumas variações pela presença ou ausência de compostos fenólicos como os taninos condensados. O objetivo deste trabalho foi avaliar a composição química-bromatológica, qualidade fermentativa e produção de gases in vitro nas silagens de sorgo granífero adicionados com diferentes níveis de guandu. O estudo foi dividido em dois experimentos. O primeiro estudo (Capítulo 3) avaliou os efeitos da adição de forragem de guandu sobre a composição química-bromatológica e fermentativas da silagem de sorgo granífero. Os tratamentos foram compostos por: T1 - Silagem com 100% de sorgo, T2 - Silagem com 25% de guandu e 75% de sorgo, T3 - Silagem com 50% de guandu e 50% de sorgo, T4 - Silagem com 75% de guandu e 25% de sorgo e T5 Silagem com 100% de guandu. As variáveis estudadas para a composição química e fermentativas das silagens foram: matéria seca (MS), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente neutro (FDA), carboidratos totais (CHOT), matéria mineral (MM), fenóis totais (FT)...

Avaliação do comportamento competitivo de raízes de ervilha (Pisum sativum) cv. Mikado; Evaluation of the roots competitive behavior of pea (Pisum sativum) cv. Mikado

Macedo, Francynês da Conceição Oliveira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 10/06/2011 PT
Relevância na Pesquisa
36.84%
A Neurobiologia Vegetal é um recente ramo das ciências vegetais que objetiva esclarecer os complexos padrões de comportamento vegetal, no que se refere à percepção, processamento, armazenamento e transmissão de sinais na planta e entre plantas. A detecção de vizinhos, é uma capacidade que implica em auto reconhecimento, uma vez que um organismo só terá sucesso em interações competitivas se for capaz de auto/não-auto discriminação. Assim, objetivou-se com este trabalho verificar se raízes de ervilha (Pisum sativum) cv. Mikado apresentam crescimento diferenciado quando na presença de raízes da mesma planta, e de raízes de outras plantas, mas pertencentes ao mesmo genótipo, para que se possa averiguar sua capacidade de auto/não-auto discriminação. Além disso, avaliou-se também o crescimento da parte aérea para observar em que grau a presença de plantas vizinhas pode influenciar o desenvolvimento vegetativo de plantas de ervilha. Quatro dias após a germinação, plântulas de Pisum sativum cv. Mikado tiveram a raiz principal cortada 5 mm abaixo do hipocótilo. Passados sete dias, foram retiradas as raízes secundárias, deixando-se apenas duas raízes, de igual tamanho, por planta (split-root). Plantas com duas raízes iguais foram replantadas...

Production and physicochemical properties of resistant starch from hydrolysed wrinkled pea starch

Polesi, Luis F.; Sarmento, Silene B. S.; Franco, Celia M. L.
Fonte: Wiley-Blackwell Publicador: Wiley-Blackwell
Tipo: Artigo de Revista Científica Formato: 2257-2264
ENG
Relevância na Pesquisa
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 07/52986-6; Processo FAPESP: 07/58577-0; The content and physicochemical properties of resistant starches (RS) from wrinkled pea starch obtained by different molecular mass reduction processes were evaluated. Native and gelatinised starches were submitted to acid hydrolysis (2 m HCl for 2.5 h) or enzymic hydrolysis (pullulanase, 40 U g(-1) for 10 h), followed by hydrothermal treatment (autoclaving at 121 degrees C for 30 min), refrigeration (4 degrees C for 24 h) and lyophilisation. Native starch showed RS and total dietary fibre contents of 39.8% and 14.3%, respectively, while processed ones showed values from 38.5% to 54.6% and from 22.9% to 37.1%, respectively. From these, the highest contents were among acid-modified starches. Processed starches showed endotherms between 144 and 166 degrees C, owing to the amylose retrogradation. Native and processed starches showed low viscosity, which is inversely proportional to the RS concentration in samples. The heat treatment promoted an increase in the water absorption index. The pea starch is a good source for obtaining resistant starch by acid hydrolysis.

Possible effects of climate change on the early development of pea, broad bean, maize and sunflower in Mediterranean areas.

Andrade, José; Abreu, Francisco
Fonte: ICB Congress Secretariat, 2008 Publicador: ICB Congress Secretariat, 2008
Tipo: Aula
POR
Relevância na Pesquisa
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In Mediterranean-type climates crop yield depends strongly on the early development of individual plants. Extreme variations of soil temperature close to the surface of bare soils and fast changes of soil water content due to irregular rainfall and high evaporative demand reduce the success of germination, emergence and early leaf production, reducing crop productivity. According to IPCC Fourth Assesment report, climate change in Southern Europe “is projected to worsen hygrometric and thermal conditions (high temperature and drought) and to reduce water availability” and, consequently, to decrease general crop productivity. The aim of this study was to evaluate the thermal conditions in the top soil layer under different climate change scenarios, and their effects on the early development of some representative crops of Mediterranean agriculture (pea, broad bean, maize and sunflower). For this purpose, soil temperatures near the surface of a Luvisol and a Vertisol during the usual sowing season of winter (October) and summer crops (April) were compared to those recorded in the air above ground, to forecast future values. The impact of simulations on the speed, size and dispersion of germination, emergence and early leaf production was estimated using values of bioclimatic parameters of the crops (cardinal temperatures and thermal times for different phases of establishment) found in the literature. In both seasons...

Gelled vegetable desserts containing pea protein, k-carrageenan and starch

Sousa, Isabel; Nunes, M.C.; Raymundo, Anabela
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
Publicado em //2006 ENG
Relevância na Pesquisa
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Due to recent animal diseases, cholesterol in take worries and strong demand for healthy food, there is a greater pressure for the direct consumption of vegetable proteins in food products. In this work, the objective is to develop alternative of strictly vegetable origin desserts based on gelled systems with required physical structure and perceived texture. For this reason, it is important to control the properties of the biopolymer mixtures and understand the phase separation behaviour un- der different physicochemical conditions. The firmness and storage modulus of different formulations of pea protein/k-carrageenan/starch systems processed and cooled at different conditions are compared with those parameters obtained for commercial products. Formulation and thermal conditions were determined to influence the texture and storage modulus of the mixed systems. Confocal microscopic images showed that phase separation between pea protein and k-carrageenan takes place, leading to the formation of two network systems. The binding of water effect, of the starch swollen granules, promotes the concentration of pea protein and k-carrageenan, reinforcing the gel structure.

Rheological behaviour and microstructure of pea protein/K-carrageenan/starch gels with different setting conditions

Sousa, Isabel; Nunes, M.C.; Raymundo, Anabela
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em //2006 ENG
Relevância na Pesquisa
36.99%
The aim of this work was to study the rheological properties and microstructure of pea protein/k-carrageenan/starch gels as affected by different cooling conditions. Dynamic oscillatory measurements for mixed gels, cooled at different rates, were conducted in a stress- controlled rheometer in order to clarify the kinetics of gel formation and characterise the structure of the matured gels. Texture parameters were determined from the texture profile analysis using a texturometer. Microstructure was observed using confocal laser scanning microscopy. In order to understand the role of each hydrocolloid on the gel network, rheology of single component gels were also studied. The results showed that gel setting conditions had a significant influence on the phase separation process of pea protein and k-carrageenan, and hence on the final structure and textural properties of the mixed gel. Slower cooling promotes an extensive phase separation between pea protein and k-carrageenan. Consequently, the size of pea protein aggregates was larger at the lowest cooling rate and decreased with increasing cooling rate. At faster cooling, an increase in elastic modulus and texture parameters of the gels were observed. Comparison of cooling and maturation profiles for the mixed system with those obtained for the individual components suggested that the gelation mechanism of k-carrageenan should govern that of the multicomponent gel.

Protein Kinase B/Akt Binds and Phosphorylates PED/PEA-15, Stabilizing Its Antiapoptotic Action

Trencia, Alessandra; Perfetti, Anna; Cassese, Angela; Vigliotta, Giovanni; Miele, Claudia; Oriente, Francesco; Santopietro, Stefania; Giacco, Ferdinando; Condorelli, Gerolama; Formisano, Pietro; Beguinot, Francesco
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /07/2003 EN
Relevância na Pesquisa
27.24%
The antiapoptotic protein PED/PEA-15 features an Akt phosphorylation motif upstream from Ser116. In vitro, recombinant PED/PEA-15 was phosphorylated by Akt with a stoichiometry close to 1. Based on Western blotting with specific phospho-Ser116 PED/PEA-15 antibodies, Akt phosphorylation of PED/PEA-15 occurred mainly at Ser116. In addition, a mutant of PED/PEA-15 featuring the substitution of Ser116→Gly (PEDS116→G) showed 10-fold-decreased phosphorylation by Akt. In intact 293 cells, Akt also induced phosphorylation of PED/PEA-15 at Ser116. Based on pull-down and coprecipitation assays, PED/PEA-15 specifically bound Akt, independently of Akt activity. Serum activation of Akt as well as BAD phosphorylation by Akt showed no difference in 293 cells transfected with PED/PEA-15 and in untransfected cells (which express no endogenous PED/PEA-15). However, the antiapoptotic action of PED/PEA-15 was almost twofold reduced in PEDS116→G compared to that in PED/PEA-15WT cells. PED/PEA-15 stability closely paralleled Akt activation by serum in 293 cells. In these cells, the nonphosphorylatable PEDS116→G mutant exhibited a degradation rate threefold greater than that observed with wild-type PED/PEA-15. In the U373MG glioma cells, blocking Akt also reduced PED/PEA-15 levels and induced sensitivity to tumor necrosis factor-related apoptosis-inducing ligand apoptosis. Thus...

Genetic distance detected with RAPD markers among selected Australian commercial varieties and boron-tolerant exotic germplasm of pea (Pisum sativum L.)

Bagheri Kazemabad, A.; Paull, J.; Langridge, P.; Rathjen, A.
Fonte: Kluwer Academic Publishers Publicador: Kluwer Academic Publishers
Tipo: Artigo de Revista Científica
Publicado em //1995 EN
Relevância na Pesquisa
36.84%
The optimisation of polymerase chain reaction (PCR) for random amplified polymorphic DNA (RAPD) analysis in pea was investigated and the results were applied to an analysis of five representative Australian varieties and five selected boron-tolerant accessions derived from different geographical regions. Genotypes were compared using 34 random primers (Operon Technologies, Alameda, CA) which generated 180 polymorphic bands. Genetic similarity among genotypes was estimated on the basis of the percentage of common bands between genotypes and a dendrogram was constructed by the unweighted pair grouping method. A pattern of RAPD reaction corresponding to two main groups was discerned. The genetic divergence between Australian varieties and the boron-tolerant accessions suggests an intensive back-crossing programme would be required to transfer boron tolerance to a locally adapted genetic background. Our results show RAPD to be useful for clarifying phylogenic relationships within a species and also to provide useful genetic markers for varietal identification in pea.; A. Bagheri, J. G. Paull, P. Langridge and A. J. Rathjen

Variation in tolerance to radiant frost at reproductive stages in field pea germplasm

Shafiq, S.; Mather, D.; Ahmad, M.; Paull, J.
Fonte: Kluwer Academic Publ Publicador: Kluwer Academic Publ
Tipo: Artigo de Revista Científica
Publicado em //2012 EN
Relevância na Pesquisa
36.84%
Radiant frost is a major abiotic stress, particularly at the reproductive stage, in field pea (Pisum sativum L.) grown in Mediterranean environments. Here, response to frost was studied for flowering stage (FS) organs (buds, flowers and set pods) and pod development stage (PDS) organs (flat, swollen and mature pods) under controlled conditions, with plants exposed to a minimum temperature of –4.8°C for 4 h. This frost treatment adversely affected seed yield through (i) abortion of buds, flowers and set pods (ii) death of pods and (iii) reduction in seed size. FS organs were more sensitive to frost than PDS organs. Genetic variation was observed among 83 accessions collected from 34 countries worldwide for survival of FS buds, flowers and set pods. In 60 of 83 accessions, no buds, flowers or set pods survived the frost treatment. Five accessions: ATC 104 (origin: United Kingdom), ATC 377 (Estonia), ATC 968 (Italy), ATC 3992 (Kazakhstan) and ATC 4204 (China), showed the highest frost tolerance of FS organs and lowest numbers of abnormal seeds. The frost tolerant accessions identified in this study may be useful as parents for breeding field pea varieties that will be less likely to suffer yield loss due to radiant frost during the reproductive stage.; Shaista Shafiq...

Epidemiology and management of ascochyta blight of field pea (Pisum sativum) in South Australia.

Davidson, Jennifer Anne
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2012
Relevância na Pesquisa
37.17%
Ascochyta blight disease (synonym: blackspot) of field pea has worldwide distribution and regularly causes AUD$25 million loss per annum in Australian field pea (Pisum sativum) crops. This study provides new information on the causal pathogens and management strategies to reduce loss from this disease. Research involving sowing dates, genotypes and fungicide treatments was conducted to identify optimal management strategies. Earlier sowing generally resulted in higher yield except when ascochyta blight was severe. Yield response to fungicide application varied with disease severity, sowing date and genotype. The optimum sowing period was within a week of the first autumn rains in low rainfall regions and 3 weeks after the first autumn rains in medium and medium - high rainfall regions. Earlier flowering genotypes were the highest yielding particularly when sown early and subjected to strategic fungicide applications. The pathogen, Phoma koolunga, was recognised for the first time as a component of the ascochyta blight disease complex in southern Australia. The species was described morphologically. Sequences of the internal transcribed spacer region were distinct from those of the accepted causal pathogens of ascochyta blight of field pea viz. Didymella pinodes...

Reproductive frost tolerance in field pea (Pisum sativum L..

Shafiq, Shaista
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2012
Relevância na Pesquisa
37.13%
Radiant frost during spring is a significant problem for field pea (Pisum sativum L.) grown in Mediterranean environments as plants are at the vulnerable reproductive stage when frost occurs. In such environments, radiant frost events after the commencement of flowering of field pea may lead to severe frost injuries on plants, and can adversely affect the grain yield. Despite the importance of the impact of frost on grain yield, no dedicated study has been conducted on reproductive frost tolerance (RFT) in field pea. One aim of this research was to develop a simple and reliable screening method to evaluate frost tolerance of eight reproductive organs (from immature buds to mature pods) which are often present at the same time on a single plant. A controlled environment screening method that exposed plants to a defined temperature regime, including a minimum temperature of -4.8°C for 4 hr, was developed. A scoring key was devised to record frost symptoms on each reproductive organ, and five categories were defined to evaluate frost damage on seeds. Using this screening method, a diverse collection of germplasm was screened, including 83 accessions sourced from high altitude and frost prone areas in 39 countries. A locally adapted variety...

Detección de carga espacial acumulada en polietileno mediante la técnica del pulso electroacústico (PEA)

Fernández Rodríguez, Sebastián
Fonte: Universidade Carlos III de Madrid Publicador: Universidade Carlos III de Madrid
Tipo: info:eu-repo/semantics/bachelorThesis; info:eu-repo/semantics/masterThesis Formato: application/octet-stream; application/octet-stream; application/pdf
SPA
Relevância na Pesquisa
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Hoy en día parece difícil vivir sin el uso de sistemas eléctricos, utilizados por los usuarios bien de forma directa (electrodomésticos, herramientas, etc), o indirecta (transformadores de potencia, generadores, líneas eléctricas de transporte o distribución, etc). Para un correcto funcionamiento de cualquier dispositivo eléctrico es necesario un sistema de aislamiento adecuado. Los sistemas de aislamiento proporcionan seguridad a los usuarios y viabilidad a los dispositivos eléctricos. La vida útil de un dispositivo eléctrico depende en buena medida de la vida útil de sus aislamientos. Los aislamientos eléctricos tienen la función de impedir el transporte de corriente eléctrica entre elementos sometidos a grandes diferencias de potencial; por ello deben soportar esfuerzos eléctricos durante su vida útil (por ejemplo un aislante de un cable de transporte en Alta Tensión). Estos esfuerzos provocan su degradación paulatina, pudiendo dar lugar a su ruptura anticipada. Por tanto es necesario investigar factores que degradan los aislamientos, y adquirir conocimientos a nivel científico mediante los cuales se puedan mejorar las cualidades de un material aislante, y favorecer y prolongar el uso del dispositivo eléctrico que protege. Un factor clave en la degradación de un aislamiento es la carga eléctrica espacial que puede aparecer en determinadas regiones de un aislante cuando éste soporta dichos esfuerzos eléctricos...

Propriedades de barreira e solubilidade de filmes de amido de ervilha associado com goma xantana e glicerol; Barrier properties of films of pea starch associated with xanthan gum and glycerol

MATTA JR, Manoel D. da; SARMENTO, Silene B. S.; SARANTÓPOULOS, Claire I. G. L.; ZOCCHI, Silvio S.
Fonte: Associação Brasileira de Polímeros Publicador: Associação Brasileira de Polímeros
Tipo: Artigo de Revista Científica
POR
Relevância na Pesquisa
36.99%
O objetivo do trabalho foi avaliar as propriedades de barreira e a solubilidade de biofilmes obtidos a partir de amido de ervilha de alto teor de amilose em associação à goma xantana e glicerol. Soluções filmogênicas (SF) com diferentes teores de amido de ervilha (3, 4 e 5%), goma xantana (0, 0,05 e 0,1%) e glicerol (proporção glicerol-amido de 1:5 P/P) foram estudadas. As SF foram obtidas por ebulição (5 minutos), seguida de autoclavagem por 1 hora a 120 ºC e os filmes foram preparados por casting. O aumento da concentração de amido e de glicerol na composição causou aumento da espessura e da solubilidade dos filmes em água. O plastificante gerou ainda elevação dos coeficientes de permeabilidade ao vapor d'água e ao oxigênio. O aumento da concentração da goma xantana não interferiu nas propriedades estudadas. Os biofilmes obtidos a partir de amido de ervilha verde, associado ou não à goma xantana e glicerol, se comparados com filmes de amido de ervilha amarelas e outras fontes de amido, apresentaram boa barreira ao oxigênio e ao vapor d'água e baixa solubilidade em água.; The aim of this work was to evaluate the barrier properties and solubility of biofilms made from wrinkled pea starch with high amylose content in association with xanthan gum and glycerol. Filmogenic solution (FS) with different levels of pea starch (3...

Effects of black-eyed pea trypsin/chymotrypsin inhibitor on proteolytic activity and on development of Anthonomus grandis

Franco, Octávio L.; Santos, Roseane C. dos; Batista, João A.N.; Mendes, Ana Cristina M.; Araújo, Marcus Aurélio M. de; Monnerat, Rose G.; Grossi-de-Sá, Maria Fátima; Freitas, Sônia M. de
Fonte: Universidade Católica de Brasília Publicador: Universidade Católica de Brasília
Tipo: Artigo de Revista Científica Formato: Texto
EN
Relevância na Pesquisa
36.71%
The cotton boll weevil Anthonomus grandis (Boheman) is one of the major pests of cotton (Gossypium hirsutum L.) in tropical and sub-tropical areas of the New World. This feeds on cotton floral fruits and buds causing severe crop losses. Digestion in the boll weevil is facilitated by high levels of serine proteinases, which are responsible for the almost all proteolytic activity. Aiming to reduce the proteolytic activity, the inhibitory effects of black-eyed pea trypsin/chymotrypsin inhibitor (BTCI), towards trypsin and chymotrypsin from bovine pancreas and from midguts of A. grandis larvae and adult insects were analyzed. BTCI, purified from Vigna unguiculata (L.) seeds, was highly active against different trypsin-like proteinases studied and moderately active against the digestive chymotrypsin of adult insects. Nevertheless, no inhibitory activity was observed against chymotrypsin from A. grandis larval guts. To test the BTCI efficiency in vivo, neonate larvae were reared on artificial diet containing BTCI at 10, 50 and 100 mM. A reduction of larval weight of up to approximately 54% at the highest BTCI concentration was observed. At this concentration, the insect mortality was 65%. This work constitutes the first observation of a Bowman–Birk type inhibitor active in vitro and in vivo toward the cotton boll weevil A. grandis. The results of bioassays strongly suggest that BTCI may have potential as a transgene protein for use in engineered crop plants modified for heightened resistance to the cotton boll weevil.

The effect of cultivar on the rate and extent of combining pea straw degradability examined in vitro using the Reading Pressure Technique

Mould, Fergus L.; Hervás, Gonzalo; Owen, Emyr; Wheeler, Tim R.; Smith, N. O.; Summerfield, R. J.
Fonte: Blackwell Publishing Publicador: Blackwell Publishing
Tipo: Artículo Formato: 22195 bytes; application/pdf
ENG
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9 pages, 10 tables.-- Available online 13 Feb. 2002.; The definitive version is available at: http://www.blackwell-synergy.com; Although about 225 000 t dry matter (DNA) pea straw is produced annually in the UK, no recent data exist with respect to its nutritive value when offered to ruminants. This study, to examine field pea crop waste (straw) from 12 commercial cultivars of combining peas, was therefore undertaken when material grown as part of a European field pea network trial at Reading in 1998 was made available. The straw was subjected to chemical analysis and examined in vitro using the Reading Pressure Technique to generate gas production profiles and to determine built rate and extent of DM arid organic matter degradation. Ash and crude protein (CP) concentrations (69 and 65 g kg(-1) DM) were generally lower than published values for legume straws but higher, especially CP, than cereal straws. Neutral-detergent fibre concentrations were slightly lower, and metabolizable energy (ME) values, derived from degradable organic matter assessed in vitro (iOMD), higher than cereal straws, ranging from 6.0 to 8.1 MJ ME kg(-1) DM. Both cumulative gas production and rate of gas release varied significantly between cultivars, as did degradation with 96-h iOMD values ranging from 468 to 602 g kg(-1). By combining these with the quantity of material harvested...

Practical importance of numerical taxonomy as a useful tool in the classification of pea landraces for their different uses

Amurrio, J. M.; Ron Pedreira, Antonio Miguel de; Casquero, P. A.
Fonte: Consejo Superior de Investigaciones Científicas (España) Publicador: Consejo Superior de Investigaciones Científicas (España)
Tipo: Artículo Formato: 247304 bytes; application/pdf
ENG
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Seventy two landraces plus four commercial varieties studied in 1989-1990 for twenty quantitative and four multistate traits were grouped into seven clusters of populations according to their earliness, shoot height, foliage area, pod quality characters, seed quality and some components of yield (number of seed per pod and number of ovules per pod). The grouping pattern of varieties (cluster) did not reflect geographical origin. From the practical point of view, dendrogram based on 24 characters provided taxonomically useful results, since there are there important groups, the first one Group I, includes varieties which could be used for combining(livestock), the second one Group II, includes cultivars which could be used as fresh pea and the last one Group IV, includes cultivars which could be used for their tender green pods.; This work was a part of a thesis submitted in fulfilment of the requirements for the M.S. degree and supported by a grant from the International Center for Advanced Mediterranean Agronomic Studies; Peer reviewed

Inclusion of field pea hay (Pisum sativum L.) and in vitro gas production in diets for growing lambs

González García,Ulises Alejandro; González Ronquillo,Manuel; Estrada Flores,Julieta G.; Bastida García,Jorge Luis; Pecador Salas,Nazario; Salem,Abdel-Fattah Z.M.
Fonte: Universidad Autónoma de Yucatán, Facultad de Medicina Veterinaria Publicador: Universidad Autónoma de Yucatán, Facultad de Medicina Veterinaria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 EN
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The use of byproducts such as field pea {Pisum sativum) is an alternative to feed sheep, the objective of this study was to evaluate the intake and digestibility in sheep fed different levels of field pea hay (FPH). Twenty Rambouillet lambs (with an initial BW of 26.0 ± 0.43 kg) were fed to one of four treatments of 5 animals of each (0%, 25%, 50% and 75% inclusion of PH as dry matter basis). The content of CP for the FPH was 8%. There were no significant differences (P>0.1) between treatments for DM intake (947.6 ± 32.3 g/d), OM (856.7 ± 24.8) and NDF (583.5 ± 30.6), as well as digestibility (g/lOOg) of DM (50.3 ± 1.0), OM (49.4 ± 2.38) and NDF (57.0 ± 2.23). N intake was lower (P<0.05) for PH 25% compared to 0%, no significant differences (P>0.1) for N excretion in feces, urine and N balance between treatments. In vitro gas production in the fraction A (ml gas / g DM incubated) was lower in FPH compared to corn stover (CS) and corn grain (CG). Fraction b was higher (P <0.05) for FPH compared to the other ingredients, however there were no differences (P>0.1) between CS and CG for fraction c. Gas production (ml gas / g DM disappeared) was lower in FPH compared to CS and CG. In vitro DM disappearance was lower for FPH with respect to CG...