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Combination of Multiple Molecular Markers Can Improve Prognostication in Patients With Locally Advanced and Lymph Node Positive Bladder Cancer

SHARIAT, Shahrokh F.; CHADE, Daher C.; KARAKIEWICZ, Pierre I.; ASHFAQ, Raheela; ISBARN, Hendrik; FRADET, Yves; BASTIAN, Patrick J.; NIELSEN, Matthew E.; CAPITANIO, Umberto; JELDRES, Claudio; MONTORSI, Francesco; LERNER, Seth P.; SAGALOWSKY, Arthur I.; COT
Fonte: ELSEVIER SCIENCE INC Publicador: ELSEVIER SCIENCE INC
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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Purpose: We tested whether the combination of 4 established cell cycle regulators (p53, pRB, p21 and p27) could improve the ability to predict clinical outcomes in a large multi-institutional collaboration of patients with pT3-4N0 or pTany Npositive urothelial carcinoma of the bladder. We also assessed whether the combination of molecular markers is superior to any individual biomarker. Materials and Methods: The study comprised 692 patients with pT3-4N0 or pTany Npositive urothelial carcinoma of the bladder treated with radical cystectomy and bilateral lymphadenectomy (median followup 5.3 years). Scoring was performed using advanced cell imaging and color detection software. The base model incorporated patient age, gender, stage, grade, lymphovascular invasion, number of lymph nodes removed, number of positive lymph nodes, concomitant carcinoma in situ and adjuvant chemotherapy. Results: Individual molecular markers did not improve the predictive accuracy for disease recurrence and cancer specific mortality. Combination of all 4 molecular markers into number of altered molecular markers resulted in significantly 1 higher predictive accuracy than any single biomarker (p < 0.001.). Moreover addition of number of altered molecular markers to the base model significantly improved the predictive accuracy for disease recurrence (3.9%...

De novo transcriptome analysis of Hevea brasiliensis tissues by RNA-seq and screening for molecular markers

Salgado, Leonardo Rippel; Koop, Daniela Martins; Pinheiro, Daniel Guariz; Rivallan, Ronan; Le Guen, Vincent; Nicolás, Marisa Fabiana; Almeida, Luiz Gonzaga Paula de; Rocha, Viviani Ribeiro; Magalhães, Milena; Gerber, Alexandra Lehmkuhl; Figueira, Anto
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
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Abstract Background The rubber tree, Hevea brasiliensis, is a species native to the Brazilian Amazon region and it supplies almost all the world’s natural rubber, a strategic raw material for a variety of products. One of the major challenges for developing rubber tree plantations is adapting the plant to biotic and abiotic stress. Transcriptome analysis is one of the main approaches for identifying the complete set of active genes in a cell or tissue for a specific developmental stage or physiological condition. Results Here, we report on the sequencing, assembling, annotation and screening for molecular markers from a pool of H. brasiliensis tissues. A total of 17,166 contigs were successfully annotated. Then, 2,191 Single Nucleotide Variation (SNV) and 1.397 Simple Sequence Repeat (SSR) loci were discriminated from the sequences. From 306 putative, mainly non-synonymous SNVs located in CDS sequences, 191 were checked for their ability to characterize 23 Hevea genotypes by an allele-specific amplification technology. For 172 (90%), the nucleotide variation at the predicted genomic location was confirmed...

Variabilidade genética e estimativa da taxa de cruzamento do pinhão manso (Jatropha curcas L.) empregando marcadores moleculares; Genetic variability and estimation of outcrossing rate of physic nut (Jatropha curcas L.) using molecular markers

Bressan, Eduardo de Andrade
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 27/01/2012 PT
Relevância na Pesquisa
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O pinhão manso é uma pequena árvore tropical que adquiriu importância econômica pelo conteúdo de óleo em suas sementes e pela possibilidade de sua utilização para produção de biocombustível. As sementes e o óleo do pinhão manso são tóxicos devido principalmente à presença de ésteres de forbol, o que dificulta a sua utilização direta para o consumo humano e também dos resíduos para a alimentação animal. A falta de programas de melhoramento e cultivares comerciais e problemas com pragas e doenças estão desestimulando o cultivo do pinhão manso pelo mundo. Por se tratar de uma espécie semi-domesticada, a utilização de marcadores moleculares como ITS, PCR-RFLP, microssatélites e TRAP poderia auxiliar nos estudos de diversidade genética, visando o desenvolvimento de variedades adaptadas às necessidades dos agricultores. O objetivo deste estudo foi caracterizar a variabilidade genética de acessos de pinhão manso depositados no Banco de Germoplasma da Universidade Federal de São Carlos, além de possibilitar estudos sobre as relações entre as populações, centros de diversidade e determinar o sistema reprodutivo da espécie. Os resultados são discutidos destacando que a maior parte da diversidade encontra-se entre as populações estudadas. Os resultados derivados dos quatro marcadores utilizados corroboram que o centro de diversidade da espécie possivelmente está na América...

Marcadores moleculares para a patogenia de vírus da raiva: relação entre períodos de incubação, carga viral e os genes codificadores das proteínas virais P e L; Molecular markers for the pathogenesis of rabies virus: relationship among incubation periods, viral load and the genes encoding the viral P and L proteins

Fahl, Willian de Oliveira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 01/04/2014 PT
Relevância na Pesquisa
65.88%
A raiva é uma doença aguda, progressiva e infecciosa do sistema nervoso central de mamíferos, causada pelo vírus da raiva (RABV). Embora possa ser prevenida por vacina, continua sendo um grave problema de saúde pública, além de ser responsável pela morte de seres humanos e muitos outros animais, incluindo os de interesse econômico. Este estudo teve como objetivo avaliar a relação entre polimorfismos dos genes que codificam as proteínas P e L de amostras de RABV pertencentes a variantes antigênicas 2 e 3 e períodos de incubação e títulos em camundongos. Para isso, foram selecionadas amostras isoladas de diferentes reservatórios de raiva de mamíferos das Ordens Carnivora e Chiroptera e amostras de bovinos, de áreas endêmicas para o vírus da raiva. As sequências obtidas foram utilizadas para a construção de árvores filogenéticas para procurar os padrões de segregação de linhagens. Os resultados mostraram que não houve marcadores ou polimorfismos que explicam as variações nos períodos de incubação e de letalidade entre cepas pertencentes a variantes antigênicas 2 e 3. Esta informação pode ser usada para discussões sobre a importância de reservatórios de raiva, a dinâmica do vírus da manutenção e evolução das diferentes formas desta zoonose entre os animais infectados...

Análise comparativa da pureza genética das leguminosas forrageiras e Stylosanthes capitata Vog. e Stylosanthes macrocephala M.B. Ferr. Et Sousa Costa utilizando marcadores moleculares; Comparative analysis of genetic purity of the forage legumes Stylosanthes capitata Vog. and Stylosanthes macrocephala M.B Ferr. Sousa Costa using molecular markers

Santos, Letícia Gobett dos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 30/10/2014 PT
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Leguminosas do gênero Stylosanthes são amplamente utilizadas na pecuária Brasileira e por sua alta qualidade nutricional são importantes para pastagens em consorcio com gramíneas. Um dos materiais mais cultivados é o denominado Campo Grande, lançado pela EMBRAPA Gado de Corte e formado pela mistura das espécies Stylosanthes capitata Vog. (80%) e Stylosanthes macrocephala M.B Ferreira et Sousa Costa (20%). De ambas as espécies que formam essa mistura, a espécie S. capitata vem sendo utilizada na pesquisa do Projeto Temático FAPESP Nº 08/58075-8 Experimentos FACE para analisar os efeitos do elevado CO e do aquecimento sobre a fotossíntese, expressão gênica, bioquímica, crescimento, dinâmica de nutrientes e produtividade de duas espécies forrageiras tropicais contrastantes que tem por objetivo determinar os efeitos do elevado nível de CO2 e do aquecimento nas espécies forrageiras S. capitata e Panicum maximum crescendo em consorcio. Antes do plantio, foi observado que o lote de sementes de S. capitata, enviadas gentilmente pela EMBRAPA Gado de Corte, apresentava sementes de diversa coloração desde amarelas, vermelhas até pretas. Em vista que a análise da expressão gênica das plantas submetidas aos tratamentos de CO2 e temperatura é um dos objetivos do projeto...

Expressão de marcadores moleculares em espermatogônias; Expression of molecular markers in spermatogonia

Giassetti, Mariana Ianello
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 03/06/2015 PT
Relevância na Pesquisa
65.98%
Em mamíferos, a espermatogênese é mantida pela autorrenovação e diferenciação das células-tronco espermatogoniais (SSC). Apesar da grande importância do SSC para a fertilidade masculina, em Bos taurus pouco se sabe sobre a sua identificação e biologia celular. Para roedores, mais de 30 marcadores para células germinativas indiferenciadas já foram descritos. No entanto, ainda não é conhecido um marcador específico apenas para SSC. Quase todos são também expressos por gonócitos, espermatogônias mais diferenciadas ou mesmo células somáticas. Yin Yang 2 (YY2) é um factor de transcrição expresso nas células com a morfologia de gonócitos e SSC, sendo um candidato a marcador de SSC. Assim, a identificação de novos marcadores para SSC e factores que afectam a sua expressão, tais como a idade, são fundamentais para o desenvolvimento da biotecnologia como transgenia e tratamento de infertilidade, nos quais as SSC poderiam ser ferramentas biológicos importantes. Assim, nesta tese temos duas hipóteses principais: 1) a idade do dador afeta a expressão de marcadores moleculares específicos de SSC bovinas assim como potencial de células-tronco dessas células e que as sequências de DNA em que se associa YY2 regulam a expressão génica de SSC em camundongos. Os objetivos específicos...

Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Morales, Rafael Gustavo Ferreira; Resende, Juliano Tadeu Vilela; Faria, Marcos Ventura; Andrade, Marcela Carvalho; Resende, Luciane Vilela; Delatorre, Carla Andrea; Silva, Paulo Roberto da
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
56.01%
Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers...

Historical evolution of organic matter accumulation in a coastal bay in the SW Atlantic, Brazil: use of sterols and n-alcohols as molecular markers

Richard,Eduardo C.; Hamacher,Cláudia; Farias,Cássia O.; Dore,Marina P.; Ribeiro,Natália C. M.; Passos,Michelle A.; Martinho,Plinio F.; Godoy,José M.; Carreira,Renato S.
Fonte: Sociedade Brasileira de Química Publicador: Sociedade Brasileira de Química
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2014 EN
Relevância na Pesquisa
65.88%
The deposition processes and preservation of organic matter (OM) in the sediments of Ilha Grande Bay, SW Atlantic, were evaluated based on elemental composition (C and N) and molecular markers (sterols and n-alcohols). Samples from four dated sediment cores, representing distinct levels and type of human influence, were analyzed. The concentration of total sterols ranged from 71 to 9,320 ng g-1, with 24-ethyl-cholesta-5,22-dien-3β-ol, 24-ethyl-cholesta-5-en-3β-ol and 24-ethyl-5a-cholesta-3β-ol as the most abundant compounds. The n-alcohols, with a total concentration between 130 and 15,407 ng g-1, were dominated by long-chain compounds (> C22). Assignments of the selected markers to terrestrial or marine sources were evaluated using principal component analysis (PCA). The trends and events that influenced the OM accumulation in the last decades were also revealed by the PCA, as the increasing human settlement in the region and the removal of a mangrove forest occurred between 1940 and 1960. The absence or low level of sewage contamination was indicated by the sterol coprostanol and associated indexes.

Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Morales,Rafael Gustavo Ferreira; Resende,Juliano Tadeu Vilela; Faria,Marcos Ventura; Andrade,Marcela Carvalho; Resende,Luciane Vilela; Delatorre,Carla Andrea; Silva,Paulo Roberto da
Fonte: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" Publicador: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 EN
Relevância na Pesquisa
56.01%
Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers...

Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)

Faria,Patrícia J.; Miyaki,Cristina Y.
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2006 EN
Relevância na Pesquisa
65.93%
The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven tetranucleotide repeat primers for their ability to amplify regions between microsatellite loci (inter simple sequence repeats, ISSRs). We were able to select seven markers that were variable in different degrees for three species (A. hyacinthinus, A. chloroptera and A. ararauna). The mini and microsatellites produced more polymorphic patterns than the ISSRs. The genetic variability of the species studied seems to be correlated with their endangered status.

Molecular characterization of Colletotrichum associated with Anthracnose on Capsicum chinense in the State of Amazonas.

OLIVEIRA, C. V. S. de; ALBUQUERQUE, D. M. C. de; SOUSA, N. R.; HANADA, R. E.; SILVA, G. F. da
Fonte: In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 25. Publicador: In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 25.
Tipo: Resumo em anais de congresso (ALICE)
EN
Relevância na Pesquisa
65.8%
The goal of this study was to compare different isolates of Colletotrichum from anthracnose lesion on C. chinense collected in the state of Amazonas, using molecular markers.; 2013

Use of molecular markers to identify soybean varieties: the experience of a public soybean breeding program.

FRONZA, V.; ABDELNOOR, R. V.; ARIAS, C. A. A.; PACHECO, L. G. A.; ARANTES, N. E.; ZITO, R. K.; SÁ, M. E. L.
Fonte: In: WORKING GROUP ON BIOCHEMICAL AND MOLECULAR TECHNIQUES, AND DNA-PROFILING IN PARTICULAR, 13., 2011, Brasília, DF. Geneva: UPOV, 2011. 2 p. BMT 13/25. Disponível em: . Publicador: In: WORKING GROUP ON BIOCHEMICAL AND MOLECULAR TECHNIQUES, AND DNA-PROFILING IN PARTICULAR, 13., 2011, Brasília, DF. Geneva: UPOV, 2011. 2 p. BMT 13/25. Disponível em: .
Tipo: Resumo em anais de congresso (ALICE)
EN
Relevância na Pesquisa
65.99%
The aim of this work is to demonstrate some cases where molecular markers were useful to identify soybean varieties in order to help breeders and the official service of protection of varieties in Brazil. The partnership between Embrapa/Epamig/Fundação Triângulo, located at Uberaba, Minas Gerais State, released some important soybean varieties adapted for the Brazilian Savannah region. One of the most important varieties developed by these partners, which was a very important variety in the recent soybean history in Brazil and also in the world, was MG/BR 46 (Conquista). ?Conquista? was released in 1995 and seemed to be just more one soybean variety for the use of soybean farmers in the Minas Gerais State, but the history proved that it was very different of the other varieties. With high yield stability, adapted to a broad range of environmental conditions (especially unfavourable conditions) and with a very rustic root system (including resistance to both root rot nematode species more common in Brazil, Meloidogyne javanica and M. incognita), ?Conquista? was sown in more than three million hectares in Brazil in a growing season. It is still sown widely (about 100.00 hectares in the growing season of 2010/2011) and was also largely used in crosses by Brazilian soybean breeders. A famous chapter of ?Conquista? history was the dispute between Fundação Triângulo (Minas Gerais State) and Fundação MT (Mato Grosso State)...

The effects of encoding data in diversity studies and the applicability of the weighting index approach for data analysis from different molecular markers.

FERRÃO, L. F. V.; CAIXETA, E. T.; CRUZ, C. D.; SOUZA, F. de F.; FERRAO, M. A. G.; ZAMBOLIM, Z.; SAKIYAMA, N. S.
Fonte: Plant Systematics and Evolution, New York, v. 300, p.1649-1661, feb. 2014. Publicador: Plant Systematics and Evolution, New York, v. 300, p.1649-1661, feb. 2014.
Tipo: Artigo em periódico indexado (ALICE)
EN
Relevância na Pesquisa
56.02%
The use of molecular markers to study genetic diversity represents a breakthrough in this area, because of the increase in polymorphism levels and phenotypic neutrality. Codominant markers, such as microsatellites (SSR), are sensitive enough to distinguish the heterozygotes in genetic studies. Despite this advantage, there are some studies that ignore this feature and work with encoded data because of the simplicity of the evaluation, existence of polyploids and need for the combined analysis of different types of molecular markers. Thus, our study aims to investigate the consequences of these encodings on simulated and real data. In addition, we suggest an alternative analysis for genetic evaluations using different molecular markers. For the simulated data, we proposed the following two scenarios: the first uses SNP markers, and the second SSR markers. For real data, we used the SSR genotyping data from Coffea canephora accessions maintained in the Embrapa Germplasm Collection. The genetic diversity was studied using cluster analysis, the dissimilarity index, and the Bayesian approach implemented in the STRUCTURE software. For the simulated data, we observed a loss of genetic information to the encoded data in both scenarios. The same result was observed in the coffee studies. This loss of information was discussed in the context of a plantbreeding program...

Physiological attributes of drought-adaptation and associated molecular markers in the seri/babax hexaploid wheat (Triticum aestivum, L.) population.

Olivares-Villegas, Juan Jose
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2007
Relevância na Pesquisa
65.95%
Agronomic and physiological traits associated with drought adaptation were assessed within the Seri/Babax recombinant inbred line population, derived from parents similar in height and maturity but divergent in their sensitivity to drought. Field trials under different water regimes were conducted over three years in Mexico and under rainfed conditions in Australia. Under drought, canopy temperature (CT) was the single-most drought-adaptive trait contributing to a higher performance (R2= 0.71, p<0.0001), highly heritable (h2= 0.65, p<0.0001) and consistently associated with yield phenotypically (r= -0.75, p<0.0001) and genetically [R(g)= -0.95, p<0.0001]. CT epitomises a mechanism of dehydration avoidance expressed throughout the growing season and across latitudes, which can be utilised as a selection criteria to identify high-yielding wheat genotypes or as an important predictor of yield performance under drought. Early response under drought, suggested by a high association of CT with estimates of biomass at booting (r= -0.44, p<0.0001), leaf chlorophyll (r= -0.22,p<0.0001) and plant height (r= -0.64, p<0.0001), contrast with the small relationships with anthesis and maturity (averaged, r= -0.10, p<0.0001), and with osmotic potential (r= -0.20...

Molecular markers and the management of tropical trees: The case of indigenous fruits

Jamnadass, R.; Lowe, A.; Dawson, I.
Fonte: Springer New York LLC Publicador: Springer New York LLC
Tipo: Artigo de Revista Científica
Publicado em //2009 EN
Relevância na Pesquisa
65.93%
Millions of people in the tropics benefit from the harvest, cultivation and consumption of indigenous fruit trees (IFTs). Knowledge concerning the structuring of genetic variation within these species is crucial for sustainable use and conservation, but the availability of such information has been limited. By reference to case study species that are currently the subject of active domestication initiatives, in this essay we provide guidance for the suitable application of molecular marker methods for characterising IFTs. We discuss three particular areas where molecular markers are able to provide unique insights for further promotion activities: (i) in understanding the past domestication of taxa, (ii) in determining effective population sizes; and (iii) in assessing the value of a decentralised approach for future domestication. Despite the potential utility of molecular markers, a disjunction between assessment and practical application is evident, which reflects a lack of integration of laboratory research with other species promotion activities. Greater emphasis is required on team-based research and partnerships, more work should be undertaken in agricultural landscapes, and further research on mating systems and gene flow are crucial. Our essay provides a model for molecular marker studies on other trees that are important to rural communities in the tropics.; Ramni Jamnadass...

Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius L.)

Boersma, J.; Pallotta, M.; Li, C.D.; Buirchell, B.; Sivasithamparam, K.; Yang, H.
Fonte: Cellular & Molecular Biology Letters Publicador: Cellular & Molecular Biology Letters
Tipo: Artigo de Revista Científica
Publicado em //2005 EN
Relevância na Pesquisa
65.96%
A mapping population of F8 derived recombinant inbred lines (RILs) was established from a cross between a domesticated breeding line 83A:476 and a wild type P27255 in narrow-leaf lupin (Lupinus angustifolius L.). The parents together with the 89 RILs were subjected to DNA fingerprinting using microsatellite-anchored fragment length polymorphism (MFLP) to rapidly generate DNA markers to construct a linkage map. Five hundred and twenty two unique markers of which 21% were co-dominant, were generated and mapped. Phenotypic data for the domestication traits: mollis (soft seeds), leucospermus (white flower and seed colour); Lentus (reduced pod-shattering), iucundis (low alkaloid), Ku (early flowering) and moustache pattern on seed coats; were included. Three to 7 molecular markers were identified within 5cM of each of these domestication genes. The anthracnose resistance gene Lanr1 was also mapped. Linkage groups were constructed using MapManager version QTXb20, resulting in 21linkage groups consisting of 7 or more markers. The total map length was 1543cM, with an average distance of 3.4cM between adjacent markers. This is the first published map for a lupin species. The map can be exploited for marker assisted selection for genetic improvement in lupin breeding programs.; http://www.cmbl.org.pl/vol10_nr2.html; Jeffrey G. Boersma...

Delimitando espécies : contribuição de marcadores morfológicos e moleculares para a compreensão do gênero Hermeuptychia Forster (Nymphalidae: Satyrinae: Euptychiina); Delimiting species : morphological and molecular markers contribution for the understanding of the genus Hermeuptychia Forster (Nymphalidae: Satyrinae: Euptychiina)

Noemy Seraphim Pereira
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 21/07/2011 PT
Relevância na Pesquisa
65.9%
O gênero Hermeuptychia Forster (Nymphalidae, Satyrinae, Euptychiina) está amplamente distribuído no continente Americano, desde a Argentina até o sul dos Estados Unidos. O gênero foi anteriormente considerado um complexo de espécies, e atualmente são reconhecidas oito espécies . Todas as espécies possuem um padrão alar muito parecido, o que compromete a identificação taxonômica correta. Em adição, a posição filogenética do gênero dentro da subtribo Euptychiina permanece incerta. Para o presente estudo foram obtidos espécimens de 45 localidades de cinco países, com maior ênfase em uma amostragem no Brasil. Três marcadores moleculares, dois do DNA mitocondrial (cox1 5' e nad6) e um do DNA nuclear (RpS5) foram utilizados para gerar hipóteses filogenéticas (Máxima Parcimônia e Inferência Bayesiana), para delimitar espécies, e para gerar estimativas de tempo de divergência e distribuição ancestral. Adicionalmente, o desempenho da região anterior da cox1 como 'barcode - código de barras' para delimitar as espécies de Hermeuptychia foi testado. Além disso, análise morfológica da genitália masculina foi empregada para a delimitação e identificação de espécies. Os indivíduos amostrados agruparam-se em dez clados nas análises moleculares...

Divergência genética em genótipos de cana-deaçúcar (Saccharum spp.) através de caracteres morfoagronômicos e por marcadores moleculares.; Genetic divergence in sugarcane genotypes (Saccharum spp.) through morphoagronomical characters and molecular markers.

Silva, Paulo Pedro da
Fonte: Universidade Federal de Alagoas; BR; Agronomia; Produção vegetal; Proteção de plantas; Programa de Pós-Graduação em Agronomia; UFAL Publicador: Universidade Federal de Alagoas; BR; Agronomia; Produção vegetal; Proteção de plantas; Programa de Pós-Graduação em Agronomia; UFAL
Tipo: Dissertação Formato: application/pdf
POR
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This study had as objective to estimate the genetic divergence among sugarcane genotypes by means of morphoagronomical characters and molecular markers, and to verify the relation between these procedures. An experiment was conducted in Rio Largo, AL, using a randomized block design with four repetitions. The multivariated analysis of Principal Components, the genetic divergence based on the Mahalanobis 2 ii' D Generalized Distance, and the Average Euclidean Distance Standardized were used for the analysis of the quantitative characters. Based on these distances, a grouping analysis was performed by the More Distant Neighbor method and the UPGMA method, besides Tocher for 2 ii' D . Jaccard coefficient and UPGMA grouping were used in the evaluation of the genetic divergence by molecular markers and morphologic characters. The inconsistency as to formation of different groups between the Standardized Average Euclidean Distance and 2 ii' D of Mahalanobis characterize these two estimates as measures of different dissimilarity. In the same way, the grouping techniques by the More Distant Neighbor method and by UPGMA show graphical dispersions that are not coincident, with differences in relation to the number of groups and in the grouping pattern...

Anastomosis groups and molecular variation in Pseudocercospora griseola.

SILVA, K. J. D. e; SOUZA, E. A.; FREIRE, C. N. S.; ISHIKAWA, F. H.
Fonte: Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 3, p. 7436-7445, July 2015. Publicador: Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 3, p. 7436-7445, July 2015.
Tipo: Artigo em periódico indexado (ALICE)
EN
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The fungus Pseudocercospora griseola is the causal agent of angular leaf spot, a common bean (Phaseolus vulgaris L.) disease. The successful development of angular leaf spot-resistant cultivars depends on understanding the levels of variability in pathogen populations. P. griseola shows great pathogenic and genetic variation using inoculation on differential bean cultivars and molecular markers. Nevertheless, how this variability develops is not clearly understood. Parasexuality has been presented as a mechanism used by asexual fungi to increase variation. Hyphal fusion is the first step for the parasexual cycle, and it can be considered an additional trait for population studies. The aim of this study was to identify hyphal fusion (anastomosis) among P. grisola isolates and to evaluate the variability of isolates using analyses of anastomosis groups and RAPD markers. Hyphal anastomosis was observed in all isolates. Three isolates showed 85.0% compatibility and were compatible with 17 isolates. This is the first report of the occurrence of anastomosis between P. griseola isolates. Sixteen anastomosis groups were observed and only one group was formed by five isolates (Pg-01, Pg-02, Pg-07, Pg-08, and Pg-12). There was a large number of anastomosis groups and absence of clustering among isolates for hyphal fusion...

Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Morales, Rafael Gustavo Ferreira; Resende, Juliano Tadeu Vilela; Faria, Marcos Ventura; Andrade, Marcela Carvalho; Resende, Luciane Vilela; Delatorre, Carla Andrea; Silva, Paulo Roberto da
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/12/2011 ENG
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Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers...