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Expression of proliferation associated antigens and detection of numerical chromosome aberrations in primary human liver tumours: relevance to tumour characteristics and prognosis.

Nolte, M; Werner, M; Nasarek, A; Bektas, H; von Wasielewski, R; Klempnauer, J; Georgii, A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1998 EN
Relevância na Pesquisa
46.42%
AIMS: To assess cell proliferation and the presence of numerical chromosome aberrations involving chromosomes 1 and 8 in benign and malignant liver tumours. METHODS: Cell proliferation was studied immunohistochemically in paraffin wax embedded material from 62 primary liver tumours (20 hepatocellular carcinomas, 16 cholangiocellular carcinomas, 15 liver cell adenomas, 11 focal nodular hyperplasias), and the results were compared with histological characteristics and clinical data. Copy numbers of chromosomes 1 and 8 were assessed by interphase fluorescence in situ hybridisation (FISH) with satellite probes in fresh tumour material. RESULTS: The expression of proliferation associated antigen Ki67, using the monoclonal antibody MIB-1, and proliferating cell nuclear antigen (PCNA), using the antibody PC10, was found to be significantly higher in malignant versus benign liver tumours. Neither Ki67 nor PCNA expression were independent prognostic parameters. However, there was a tendency for a worse outcome (survival < 12 months) for patients with a high MIB-1 labelling index (> 20%) compared with patients having the same tumour stage and a low MIB-1 index. Aneusomy for chromosomes 1 and 8 was demonstrated by FISH in malignant tumours (six of seven hepatocellular carcinomas...

Long-term dosing studies using mutagenic carcinogens indicate a highly significant correlation between elevations in the level of rat glutathione S-transferase P messenger RNA and liver tumours of hepatocellular origin.

Russell, S E; Pearson, C; Kelly, M; McQuaid, S; Humphries, P
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 01/01/1988 EN
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46.23%
We have investigated levels of transcript homologous with glutathione S-transferase P (GST-P; GST 7-7) in tumours and hyperplastic lesions induced in the livers of rats by long-term gavage dosing with diethylnitrosamine (DEN) and 6-p-dimethylaminophenylazobenzothiazole (6BT). Detailed histopathological examination of the livers of the 90 animals used in this study at 6-8 months after initiation of daily dosing revealed that, of the 30 animals treated with carcinogen, 15 had developed tumours or hyperplastic lesions. Of these, 11 were areas of fibrosarcoma/fibrous hyperplasia. The remaining four were hepatocellular carcinomas. Northern blotting of total RNA purified from these tissues revealed the presence of transcripts of 3 and 0.75 kb. Evidence is presented to indicate that the former is a hitherto-undetected precursor of the 3-kbp rat GST-P gene, the latter representing the previously characterized mature GST-P transcript. Large elevations of the 0.75-kb transcript (30-35-fold) were encountered in all of the hepatocellular carcinomas, but in none of the other lesions, indicating a highly significant correlation (P = less than 0.001) between high elevations in levels of GST-P mRNA and liver tumours of hepatocellular origin. Minor elevations in transcript level (less than or equal to 5-fold) were encountered in several of the non-hepatocellular lesions. In regenerating livers...

Improved characterisation of histologically proven liver tumours by contrast enhanced ultrasonography during the portal venous and specific late phase of SHU 508A

Dietrich, C F; Ignee, A; Trojan, J; Fellbaum, C; Schuessler, G
Fonte: Copyright 2004 by Gut Publicador: Copyright 2004 by Gut
Tipo: Artigo de Revista Científica
Publicado em /03/2004 EN
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56.18%
Purpose: Ultrasound is reported to be relatively unreliable in the characterisation of liver tumours. The purpose of this study was to assess the ability of contrast enhanced phase inversion ultrasound (PIUS), a new highly sensitive contrast specific technique, performed during the liver specific phase of Levovist, to differentiate between benign and malignant lesions of the liver.

The Role of Liver Transplantation in the Management of Paediatric Liver Tumours

Stringer, Mark D
Fonte: The Royal College of Surgeons of England Publicador: The Royal College of Surgeons of England
Tipo: Artigo de Revista Científica
Publicado em /01/2007 EN
Relevância na Pesquisa
46.39%
In recent years, considerable progress has been made in the treatment of children with hepatoblastoma largely due to effective pre-operative chemotherapy. Total hepatectomy and liver transplantation has emerged as an effective treatment for the small proportion of children with unresectable hepatoblastoma limited to the liver. A 5-year survival of 70% can be achieved in such cases. In contrast, the results of liver transplantation in children with hepatocellular cancer remain poor because these tumours are usually advanced with evidence of major vascular invasion and/or extrahepatic spread at the time of presentation. An exception is those children in whom the hepatocellular carcinoma is detected during surveillance of chronic liver disease – they typically have smaller tumours and frequently have a good prognosis after liver transplantation. The role of liver transplantation in children with other primary hepatic malignancies remains uncertain because experience is very limited. Liver transplantation is rarely needed in the management of children with benign liver tumours but, if other treatments have failed, it can be a life-saving intervention.

Effectiveness of isolated liver perfusion with mitomycin C in the treatment of liver tumours of rat colorectal cancer.

Marinelli, A.; Dijkstra, F. R.; van Dierendonck, J. H.; Kuppen, P. J.; Cornelisse, C. J.; van de Velde, C. J.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /07/1991 EN
Relevância na Pesquisa
46.24%
Dose limiting systemic toxicity prevents sufficient exploitation of the steep dose response relationship of most anticancer agents. In our rat liver tumour model (the CC531 colorectal carcinoma), isolated liver perfusion allows administration of higher doses of mitomycin C than hepatic artery infusion, while systemic toxicity remains minimal. To determine the temporal pattern of mitomycin C induced cytokinetic changes, we analysed flow cytometric DNA histograms of CC531 liver tumours from rats treated with high dose mitomycin C (3.2 mg kg-1) via hepatic artery infusion and sacrificed at different time intervals after treatment. Between 12 and 36 h after treatment, the fraction of cells in late S and G2/M phase had markedly increased. The effects of administration of the respective maximally tolerated doses of mitomycin C in isolated liver perfusion and via hepatic artery infusion on progression of tumour cells through the cell cycle and on gross tumour growth were compared. Isolated liver perfusion with mitomycin C resulted in a significant increase in the proportion of cells in mid and late S, and in some accumulation of cells in early S and G2/M phase at 24 and 48 h after treatment. In contrast, after hepatic artery infusion a significant increase of the fraction of cells in G2/M phase was observed at 24 h after treatment. Monitoring tumour growth after isolated liver perfusion five out of seven rats showed a complete tumour remission...

CT-guided intratumoural administration of cisplatin/epinephrine gel for treatment of malignant liver tumours

Vogl, T J; Engelmann, K; Mack, M G; Straub, R; Zangos, S; Eichler, K; Hochmuth, K; Orenberg, E
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em 12/02/2002 EN
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46.24%
To analyze prospectively the interventional and clinical aspects of computed tomography-guided direct intratumoural injection of a novel chemotherapeutic administration and the parenchymal changes of tumour and necrosis in malignant liver tumours. Eight patients with 17 colorectal liver metastases were treated with a mean of 5.1 injections and nine patients with 13 hepatocellular carcinoma nodules with a mean of 3.1 treatments with computed tomography guided local applications of a novel cisplatin/epinephrine gel. This application provides a higher local and lower systemic drug concentration. Volumes of tumour and necrosis prior and after treatment were measured by computer generated volumetric analysis. Contrast enhanced studies verified pretherapeutic viable tumour volumes with a value of 77.4 ml in the metastases and 29.2 ml in the hepatocellular carcinoma nodules. Intratumoural drug application resulted in a significant increase of necrosis and a decrease in viable tumour volume to be 68.3 ml in metastases and 14.5 ml in hepatocellular carcinoma. Local therapy control rate for the follow up to 6 months was 38 and 71% for the group of metastases and hepatocellular carcinoma, respectively. Direct intratumoural injection of cisplatin/epinepthrine injectable gel is a feasible and good tolerated method and results in the development of a statistically significant increase in necrosis in malignant liver tumours. For hepatocellular carcinoma a higher local therapy control rate compared to colorectal metastases can be reported.

Cryotherapy of Liver Tumours–A Practical Guide

Ross, W. B.; Horton, M.; Bertolino, P.; Morris, D. L.
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Publicado em //1995 EN
Relevância na Pesquisa
46.24%
The use of cryotherapy for the treatment of some unresectable liver tumours has been clearly established as a therapeutic option. Intra-operative ultrasound has enhanced the process by enabling the surgeon to identify hepatic lesions and to allow visualisation of the freezing process to ensure that the cryolesion will include the tumour mass. The purpose of this paper is to provide a practical guide to surgeons who wish to perform cryotherapy of liver tumours. Patient selection and anaesthetic considerations are important. The surgeon should be able to deal with the complications of cryotherapy, particularly the intra-operative haemorrhage which may arise from cracking of the hepatic parenchyma as the iceball thaws. Follow-up is based on tumour marker assay and imaging of the liver and repeat cryotherapy can be considered for selected cases.

Magnetic resonance-guided high-intensity focused ultrasound (MR-HIFU) ablation of liver tumours

Wijlemans, J.W.; Bartels, L.W.; Deckers, R.; Ries, M.; Mali, W.P.Th.M.; Moonen, C.T.W.; van den Bosch, M.A.A.J.
Fonte: e-Med Publicador: e-Med
Tipo: Artigo de Revista Científica
Publicado em 28/09/2012 EN
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56.2%
Recent decades have seen a paradigm shift in the treatment of liver tumours from invasive surgical procedures to minimally invasive image-guided ablation techniques. Magnetic resonance-guided high-intensity focused ultrasound (MR-HIFU) is a novel, completely non-invasive ablation technique that has the potential to change the field of liver tumour ablation. The image guidance, using MR imaging and MR temperature mapping, provides excellent planning images and real-time temperature information during the ablation procedure. However, before clinical implementation of MR-HIFU for liver tumour ablation is feasible, several organ-specific challenges have to be addressed. In this review we discuss the MR-HIFU ablation technique, the liver-specific challenges for MR-HIFU tumour ablation, and the proposed solutions for clinical translation.

A Surgical Device for Radiofrequency Ablation of Large Liver Tumours

dos Santos, I; Correia, D; Soares, A J M; Góes, J A; da Rocha, A F; Schutt, D; Haemmerich, D
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.26%
Radiofrequency ablation has become an accepted treatment option of patients with primary and metastatic liver tumours. We propose an ablation electrode array consisting of 4–8 blade shaped electrodes arrangend in a circular geometry for the treatment of large liver tumours. We developed a 3D code based on the finite difference method to evaluate the effect of diFferent numbers of electrodes (4, 6 and 8) and electrode distance on lesion size. The configuration with six electrodes can ablate a volume of 70 × 70 × 40 mm3 in approximately 5 minutes, with tissue temperature above 50 °C throughout the treatment volume. We then performed an experimental study in polyacrylamide gel in order to validate the theoretical results. The average temperature error between the simulation and the experiment was 3.8% at the center of the electrode array. This study shows that the proposed device potencially allows more rapid treatment of large tumours compared to current radiofrequency ablation devices.

Detecting and characterising small liver tumours

Robinson, P. J. A.
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.32%
The successful treatment of liver tumours is more likely if they are found early. The use of high-resolution CT and MRI with contrast enhancement allows more sub-centimetre liver lesions to be detected, but some small tumours remain occult even at surgery with intra-operative sonography. An indication of the accuracy of imaging in detecting liver metastases may be given by the proportion of lesions found which are under 1–2 cm in size. The characterisation of small lesions remains problematic on CT, with benign and malignant tumours showing overlapping imaging features. However, with appropriate use of chemical shift, heavy T2 weighting, gadolinium enhancement, and liver-specific contrast agents, a carefully tailored MRI examination will usually produce diagnostic appearances.

A new treatment for unresectable liver tumours: long-term studies of electrolytic lesions in the pig liver

Wemyss-Holden, S.; Robertson, G.; Dennison, A.; Vanderzon, P.; de la M Hall, P.; Maddern, G.
Fonte: Portland Press Publicador: Portland Press
Tipo: Artigo de Revista Científica
Publicado em //2000 EN
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56.31%
The majority of liver tumours are inoperable and an alternative treatment to surgical resection is urgently needed. Electrolysis has been investigated in a rat model and the procedure is safe, with accurate and predictable effects. The necrosis produced has also been shown to cause destruction of tumour deposits in the rat liver. A similar evaluation in a large animal model was necessary before clinical trials could commence. Using platinum electrodes connected to a d.c. generator, areas of hepatic necrosis were created in the pig liver. Animals were killed at various time points after treatment to assess the extent of healing. Treatment was uneventful and all animals made a full recovery. No animal died from the treatment or had to be killed prematurely. After 2 days of treatment, healing was minimal but at successive time points there was progressive evidence of healing, such that after 4 months, the original electrolytic lesion was greatly reduced in size and the large area of necrosis seen at the early time points was largely replaced by a fibrous scar with only small islands of necrotic tissue. In a large animal model, electrolysis is a safe method for creating areas of hepatic necrosis. The lesions heal with time and are associated with minimal morbidity. The results support a trial of electrolysis in patients with unresectable liver tumours.

The emergent role of focal liver ablation techniques in the treatment of primary and secondary liver tumours

Garcea, G.; Lloyd, T.; Aylott, C.; Maddern, G.; Berry, D.
Fonte: Pergamon-Elsevier Science Ltd Publicador: Pergamon-Elsevier Science Ltd
Tipo: Artigo de Revista Científica
Publicado em //2003 EN
Relevância na Pesquisa
56.3%
Only 20% of patients with primary or secondary liver tumours are suitable for resection because of extrahepatic disease or the anatomical distribution of their disease. These patients could be treated by ablation of the tumour, thus preserving functioning liver. This study presents a detailed review of established and experimental ablation procedures. The relative merits of each technique will be discussed and clinical data regarding the efficacy of the techniques evaluated. A literature search from 1966 to 2003 was undertaken using Medline, Pubmed and Web of Science databases. Keywords were Hepatocellular carcinoma, liver metastases, percutaneous ethanol injection, cryotherapy, microwave coagulation therapy, radiofrequency ablation, interstitial laser photocoagulation, focused high-intensity ultrasound, hot saline injection, electrolysis and acetic acid injection. Ablative techniques offer a promising therapeutic modality to treat unresectable tumours. Large-scale randomised controlled trials are required before widespread acceptance of these techniques can occur.; http://www.elsevier.com/wps/find/journaldescription.cws_home/104/description#description; G. Garcea, T.D. Lloyd, C. Aylott, G. Maddern and D.P. Berry; Copyright © 2003 Elsevier Ltd. All rights reserved.

The future of radiofrequency ablation is looking BETA : short and long term studies of bimodal electric tissue ablation (BETA) in a porcine model.

Dobbins, Christopher
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2008
Relevância na Pesquisa
46.38%
Introduction: Radiofrequency ablation (RFA) is a popular method of treating unresectable liver tumours by the use of a high frequency, alternating electrical current that heats and destroys tumour cells. The size of the ablation is limited by localised charring of adjacent tissue that prevents further conduction of the radiofrequency current. In the clinical setting, this results in increased rates of local recurrence in tumours that are greater than 3 cm in diameter as multiple, overlapping ablations need to be performed to treat the one tumour. To overcome this problem, a modified form of RFA called Bimodal Electric Tissue Ablation (BETA) has been created. BETA adds a direct electrical current to the alternating radiofrequency current, thus establishing its bimodal character. When direct currents are used in biological tissues, water is transferred from anode to cathode by a process called electro-osmosis. By attaching the cathode to the radiofrequency electrode, water is attracted to the area thus preventing tissue desiccation and charring. The BETA circuit has been constructed and tested using a porcine model. The aims of the studies are to confirm that larger ablations can be produced with the BETA system and that it is safe to use in an animal model. Three studies have been performed to test these aims in porcine liver. Methods: The first study was designed to compare sizes of the ablation produced between standard RFA and the BETA circuit. This was followed by a long-term study to assess associated changes to liver function and pathological changes within the liver as well as identifying any other treatment related morbidity. The third study assessed the difference in ablation size and safety aspects when the positive electrode of the direct current circuitry was moved from small surface area under the skin to a large surface area on the skin. Results: Ablations with significantly larger diameters are created with the BETA circuit using a multi-tine needle (49.55 mm versus 27.78 mm...

Untersuchung der differenziellen Aktivierung von zellulären Signalübertragungswegen in Maus-Lebertumoren mit Mutationen im Ha-ras-, B-raf- oder Ctnnb1-Onkogen mit Protein-Mikroarray-basierten Methoden; Analysis of the differential activation of cellular signal transduction pathways in mouse liver tumours with mutations in the Ha-ras-, B-raf- or Ctnnb1-oncogene with protein microarray based methods

Knorpp, Thomas
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
DE_DE
Relevância na Pesquisa
46.26%
Veränderungen in Signaltransduktions- und Regulationsmechanismen sind eine Voraussetzung für die Entstehung von Tumoren. Diese führen zur Deregulation von Signalwegen und machen eine Rezeptorstimulation zur Aktivierung überflüssig. Mutationsbedingte Veränderungen dieser Signalwege wurden in Lebertumoren, die in einem Tiermodell nach chemischer Kanzerogenese erhalten wurden, auf ihre Auswirkungen hinsichtlich der Aktivität und Wechselwirkungen zu anderen Signalwegen untersucht. Analysiert wurden Mutationen im Erk/MAPK- (Ha-ras- oder B-raf-Gene) und im Wnt-Signalweg (Ctnnb1), welche auch beim Menschen eine zentrale Rolle bei der Tumorentstehung einnehmen. Die betroffenen Proteine haben zentrale regulatorische Bedeutung bei der Aktivierung der Signalwege und üben einen großen Einfluss auf die Proteinexpression der betroffenen Zellen aus. Mit Protein-Mikroarrays wurden vergleichende Expressionsanalysen auf Proteinebene von katalytisch oder regulativ aktiven Proteinen durchgeführt. Auf diesen hoch parallelisierten und miniaturisierten Festphasenassays wurden Lysate von bis zu 40 Tumor- und Normalgeweben aufgebracht. Die Signalgenerierung erfolgte durch ein Antikörper-basiertes Fluoreszenzdetektionsverfahren. Über die Bestimmung der immobilisierten Proteinmenge auf der planaren Oberfläche der Mikroarray-Substrate wurde ein Verfahren zur Normierung des Assaysignals etabliert. Die Bestimmung differenzieller Expression und Modifikation wurde für Proteine des Wnt- und Erk/MAPK-Signalweges...

Untersuchungen zur Rolle von Connexin32 in der chemisch-induzierten Hepatokanzerogenese im Mausmodell; Role of Connexin32 in chemically-induced hepatocarcinogenesis in mice

Mönnikes, Oliver
Fonte: Universität Tübingen Publicador: Universität Tübingen
Tipo: Dissertation; info:eu-repo/semantics/doctoralThesis
DE_DE
Relevância na Pesquisa
46.24%
Connexin32 (Cx32) ist das in den Hepatozyten der Maus vorherrschende gap-junction-Protein. In der vorliegenden Arbeit wurde der Einfluss von Cx32 auf den Hepatokanzerogeneseprozess untersucht. Experiment I: Genetischer Hintergrund Mäuse verschiedener Stämme zeichnen sich durch unterschiedliche Lebertumor-Suszeptibilität aus. Cx32-Null-Mäuse wurden mit C57BL- (wenig) bzw. C3H-Mäusen (hoch suzeptibel) gekreuzt. Cx32Y/+ und Cx32Y/- Männchen (Cx32 ist X-chromosmal lokalisiert) der jeweiligen F1-Generation wurden einmalig mit dem Hepatokanzerogen DEN behandelt und die kanzerogene Antwort in der Leber anhand Glukose-6-Phosphatase (G-6-Pase) defizienter Läsionen quantifiziert. Hierbei zeigten Cx32Y/--Mäuse eine im Vergleich zum jeweiligen Wildtyp deutlich stärkere kanzerogene Antwort, insbesondere eine gesteigerte Wachstumsgeschwindigkeit der enzymveränderten Läsionen. Der Cx32-Gendefekt 'promoviert' unabhängig vom weiteren genetischen Hintergrund den Kanzerogeneseprozess, wobei, die weitaus stärkste Antwort in Cx32-Null-C3H-Mäusen beobachtet wurde. Mäuse dieses Stamms reagieren somit extrem sensitiv auf krebsauslösende Stoffe und sind somit potentiell zur empfindlichen Erfassung kanzerogener Wirkungen geeignet. Experiment II: Mosaik-Expression von Cx32 Die aufgrund X-chromosomaler Inaktivierung postulierte mosaikartige Expresssion von Cx32 in der Leber heterozygoter Cx32+/--Mäuse konnte im Lebernormalgewebe durch immunhistochemische Färbung nachgewiesen werden...

Electrolytic treatment of colorectal liver tumour deposits in a rat model: a technique with potential for patients with unresectable liver tumours

Wemyss-Holden, S.; Robertson, G.; de la M Hall, P.; Dennison, A.; Maddern, G.
Fonte: Karger Publicador: Karger
Tipo: Artigo de Revista Científica
Publicado em //2000 EN
Relevância na Pesquisa
56.42%
BACKGROUND/AIMS: Patients with unresectable malignant liver tumours have a poor prognosis. A technique is needed which improves long-term survival. Previous studies in the rat have shown that electrolysis is a safe, predictable and reproducible method for creating areas of necrosis in the normal rat liver. This study examined the effects of electrolysis on colorectal liver 'metastases' in the rat. METHODS: Tumours of colorectal origin were implanted into the livers of Wistar-WAG rats. Two weeks after implantation the tumours were treated with electrolysis. A direct current generator, connected to 2 platinum intrahepatic electrodes was used to examine the effects of various electrode configurations on the extent of tumour necrosis. RESULTS: Significant (p<0.001) tumour ablation was achieved with all electrode configurations. Tumour necrosis was more complete (p<0.05) with the electrodes positioned on either side of the tumour than with both electrodes placed in the centre of the tumour. Liver enzymes (AST and ALT) were significantly (p<0.001) elevated after treatment, but returned towards normal by 2 days. CONCLUSIONS: This study has shown that colorectal liver 'metastasis' can be ablated by electrolysis in a rat model. Two separate mechanisms of tumour ablation were observed: With the electrodes directly in or adjacent to the tumour...

Electrochemically-induced hepatic necrosis: the next step forward in patients with unresectable liver tumours?

Baxter, P.; Wemyss-Holden, S.; Dennison, A.; Maddern, G.
Fonte: BLACKWELL SCIENCE Publicador: BLACKWELL SCIENCE
Tipo: Artigo de Revista Científica
Publicado em //1998 EN
Relevância na Pesquisa
56.29%
BACKGROUND: The treatment of patients with unresectable liver tumours remains an unsolved clinical problem. Several methods of locoregional treatment have been developed. These methods rely mainly on direct thermal or chemical insults and consequently have their own inherent limitations in clinical usage. The 'ideal' treatment would combine the direct cytotoxic effects of chemical treatments with the relative predictability of thermal insults, without the associated complications. This study aims to investigate whether the direct chemical effect of electrolytic hepatic necrosis is associated with any heating effect, and if so, whether the temperature change is dose-dependent. METHODS: An electrolytic 'dose' sufficient to induce a localized zone of hepatic necrosis was delivered to the livers of rats and pigs via implanted platinum electrodes. RESULTS: The results showed that there was no significant temperature increase at low current levels (2-4 mA) in the rat liver. In the pig, there was a significant (P < 0.01) increase in temperature of 4.2 degrees C during electrolysis, when delivered at between 20 and 50 mA. However, such a small increase in temperature would have been insufficient to cause appreciable thermal necrosis. CONCLUSIONS: This study demonstrates that electrolysis-induced hepatic necrosis is produced without an increase in temperature; clearly cell death results from the direct effects of cytotoxic electrode products and an alteration of intracellular pH. Consequently...

Electrochemotherapy on liver tumours in rabbits.

Ramirez, L. H.; Orlowski, S.; An, D.; Bindoula, G.; Dzodic, R.; Ardouin, P.; Bognel, C.; Belehradek, J.; Munck, J. N.; Mir, L. M.
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em /06/1998 EN
Relevância na Pesquisa
46.32%
Electrochemotherapy (ECT) is a new therapeutic approach combining the effects of a low-permeant cytotoxic drug, bleomycin (BLM), administered i.v. and cell-permeabilizing electric pulses (EPs) locally delivered to tumours. The transient permeabilization of the cell membrane by the EPs allows free access of BLM to its intracellular targets, largely enhancing BLM's cytotoxic effects. ECT efficacy has been proved so far on transplanted subcutaneous murine tumours and on subcutaneous metastases in humans. Here, we present the first study of the effects of ECT on tumours transplanted to livers in rabbits. We used a recently developed EP applicator consisting of an array of parallel and equidistant needles to be inserted in tissues. Effects of EPs alone or of ECT were assessed by histological analysis, tumour growth rates and survival of the treated animals. A transient blood hypoperfusion was seen in the electropulsed areas, with or without BLM, related to EP-dependent vasoconstriction but this had no major effects on cell survival. Long-term effects depended on the presence of BLM at the time of EP delivery. Almost complete tumour necrosis was observed after ECT, resulting from both BLM direct cytotoxic effects on electropermeabilized tumour cells and indirect effects on the tumour vessels. A large reduction in tumour growth rate and significantly longer survival times were scored in comparison with control rabbits. Moreover...

Repeated resection for malignant liver tumours.

Moussa, M. E.; Bean, A. G.; Habib, N. A.
Fonte: Royal College of Surgeons of England Publicador: Royal College of Surgeons of England
Tipo: Artigo de Revista Científica
Publicado em /09/1995 EN
Relevância na Pesquisa
46.27%
Fifteen repeat hepatic resections were performed on 12 patients with either recurrent or residual malignant tumours of the liver. Of these, one patient underwent three repeat resections and another underwent two. Five had primary liver liver tumours and seven had liver metastases. Planned, 'staged', repeat resections were performed on three patients because of multiple deposits of tumour, cirrhosis or extensive disease at initial presentation. There was no operative mortality. The period of follow-up from the time of repeat sections ranged between 4 months and 36 months during which two patients died from recurrent disease. The mean survival after the repeat resection was 16.8 months (range 4-36 months). Although technically demanding, repeat hepatectomy is feasible and provide similar benefits.

Liver tumours in children: current surgical management and role of transplantation

Millar,A J W
Fonte: SAMJ: South African Medical Journal Publicador: SAMJ: South African Medical Journal
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2014 EN
Relevância na Pesquisa
46.24%
This article reviews the current surgical management of liver tumours in children in the light of improved chemotherapy, surgical techniques and outcomes from transplantation. It is a principle of management that complete removal of a tumour must be achieved for cure. Neoadjuvant chemotherapy may downstage advanced local disease to enable safe curative tumour resection. When this is not achievable, transplant is indicated. Conventional indications for transplant are unresectable stages 3 and 4 tumours confined to the liver. With the realisation that lifelong immunosuppressive therapy has considerable adverse consequences, there has been a recent trend towards extreme and 'acrobatic' liver resection to avoid transplantation, but still obtain a cure. The current literature is reviewed in the light of these trends and our own experience.