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Combined glucosamine and chondroitin sulfate provides functional and structural benefit in the anterior cruciate ligament transection model

SILVA JR., Francisco Saraiva; YOSHINARI, Natalino Hajime; CASTRO, Rondinelle Ribeiro; GIRAO, Virginia Claudia Carneiro; POMPEU, Margarida Maria Lima; FEITOSA, Judith Pessoa de Andrade; ROCHA, Francisco Airton Castro
Fonte: SPRINGER Publicador: SPRINGER
Tipo: Artigo de Revista Científica
ENG
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Evidence that combined glucosamine sulfate and chondroitin sulfate (Gluchon) or isolated glucosamine (Glu) modifies joint damage in osteoarthritis (OA) is still lacking. We studied joint pain and cartilage damage using the anterior cruciate ligament transection (ACLT) model. Wistar rats were subjected to ACLT of the right knee ( OA) or sham operation. Groups received either Glu (500 mg/kg), Gluchon (500 mg/kg glucosamine +400 mg/kg chondroitin) or vehicle (non-treated-NT) per os starting 7 days prior to ACLT until sacrifice at 70 days. Joint pain was evaluated daily using the rat-knee joint articular incapacitation test. Structural joint damage was assessed using histology and biochemistry as the chondroitin sulfate ( CS) content of cartilage by densitometry (microgram per milligram dried cartilage), comparing to standard CS. The molar weight (Mw) of the CS samples, used as a qualitative biochemical parameter, was obtained by comparing their relative mobility on a polyacrylamide gel electrophoresis to standard CS. Gluchon, but not Glu, significantly reduced joint pain (P<0.05) compared to NT. There was an increase in CS content in the OA group (77.7 +/- 8.3 mu g/mg) compared to sham (53.5 +/- 11.2 mu g/mg) (P<0.05). The CS from OA samples had higher Mw (4:62 +/- 0:24 x 10(4) g/mol) compared to sham (4:18 +/- 0:19 x 10(4) g/mol) (P<0.05). Gluchon administration significantly reversed both the increases in CS content (54.4 +/- 12.1 mu g/mg) and Mw (4:18 +/- 0:2 x 104 g/mol) as compared to NT. Isolated Glu decreased CS content though not reaching statistical significance. Cartilage histology alterations were also significantly prevented by Gluchon administration. Gluchon provides clinical (analgesia) and structural benefits in the ACLT model. This is the first demonstration that biochemical alterations occurring in parallel to histological damage in OA are prevented by Gluchon administration.; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Urinary glycosaminoglycans in horse osteoarthritis. Effects of chondroitin sulfate and glucosamine

Baccarin, Raquel Y. A.; Machado, Thais S. L.; Lopes-Moraes, Ana P.; Vieira, Fabiana A. C.; Michelacci, Yara M.
Fonte: ELSEVIER SCI LTD; OXFORD Publicador: ELSEVIER SCI LTD; OXFORD
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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Our objectives were to characterize the urinary excretion of glycosaminoglycans (GAGs) in horse osteoarthritis, and to investigate the effects of chondroitin sulfate (CS) and glucosamine (GlcN) upon the disease. Urinary GAGs were measured in 47 athletic horses, 20 healthy and 27 with osteoarthritis. The effects of CS and GlcN were investigated in mild osteoarthritis. In comparison to normal, urinary GAGs were increased in osteoarthritis, including mild osteoarthritis affecting only one joint. Treatment with CS + GlcN led to a long lasting increase in the urinary CS and keratan sulfate (KS), and significant improvement in flexion test of tarsocrural and metacarpophalangeal joints was observed. In conclusion, urinary CS and KS seems to reflect the turnover rates of cartilage matrix proteoglycans, and the measurement of these compounds could provide objective means of evaluating and monitoring joint diseases. (C) 2011 Elsevier Ltd. All rights reserved.; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasilia, DF, Brazil; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasilia, DF, Brazil; Fundacao Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brasilia, SP, Brazil; Fundacao Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)...

Novos tensoativos derivados da 2-D-glucosamina; New surfactants based on 2-D-glucosamine

Bazito, Reinaldo Camino
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 13/12/2001 PT
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Foram sintetizadas duas novas séries de tensoativos de açúcar derivados da 2-D-glucosamina: os metil 2-acilamido-2-deóxi-6-O-sulfonato-D-glucopiranosídeos de sódio (aniônicos) e os cloretos de metil 2-acilamido-2,6-dideóxi-6-trimetilamônio-D-glucopiranosídeos (catiônicos). Os tensoativos aniônicos foram obtidos pela acilação da 2-D-glucosamina com cloretos de acila (com 8, 12 e 16 carbonos), seguida pela metilação desses derivados com metanol em meio ácido, e posterior sulfatação dos metil glucosídeos com complexo trióxido de enxofre-piridina. Os tensoativos catiônicos foram obtidos pela tosilação dos metil glucosídeos, seguida pela quaternização com trimetilamina e troca do contra-íon tosilato por cloreto com resina de troca-iônica. Esses tensoativos apresentaram c.m.c. similares a de outros tensoativos iônicos de cadeia hidrofóbica de igual comprimento, mas energias livres de transferência do grupo polar para a micela muito mais favoráveis. Esse fato foi atribuído à formação de ligações de hidrogênio entre os grupos polares do tensoativo na micela, e à hidrofobicidade do açúcar. As micelas formadas apresentaram números de agregação maiores que os obtidos para outros tensoativos, provavelmente devido às interações atrativas entre os grupos polares.; Two new sugar-based surfactant series were synthesized from 2-D-glucosamine: sodium methyl 2-acylamido-2-deoxi-6-O-sulfonate-D-glucopyranosides (anionic) and methyl 2-acylamido-2...

Osteoartrite experimental em ratos: efeito de sulfato de glicosamina e sulfato de condroitina sobre a incapacitação articular e a lesão de cartilagem articular; Experimental osteoarthritis in rats: evaluation of antinociceptive and chondroprotective effects of glucosamine sulfate and chondroitin sulfate

Silva Junior, Francisco Saraiva da
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 11/04/2007 PT
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OBJETIVOS: Avaliar o efeito de sulfato de glicosamina e sulfato de condroitina sobre a nocicepção e o dano da cartilagem articular em um modelo de osteoartrite experimental em ratos. MÉTODOS: Osteoartrite (OA) foi induzida em ratos Wistar machos por transecção do ligamento cruzado anterior (TLCA) do joelho direito. Um grupo falso-operado (SHAM) foi utilizado como controle. Animais OA foram tratados v.o. desde 7 dias antes, até o sacrifício, 70 dias após a TLCA, com sulfato de glicosamina 500 mg/kg (Glu), a combinação sulfato de glicosamina 500 mg/kg e sulfato de condroitina 400 mg/kg (GluChon), ou salina (OANT). Um grupo controle positivo recebeu meloxicam 6 mg/kg s.c. A dor articular foi avaliada pelo teste de incapacitação articular para ratos. Os animais foram sacrificados em diferentes períodos (7,14,28 e 70 dias) após a TLCA. A gravidade das lesões histopatológicas foi graduada nos fêmures após coloração por H&E e azul de toluidina através do escore da OARSI. A quantidade de glicosaminoglicanos (GAGs) extraídos da cartilagem articular dos côndilos femorais foi medida após eletroforese em gel de agarose. O tamanho molecular dos GAGs foi medido após eletroforese em gel de poliacrilamida. A liberação de NO no líquido sinovial foi medida 7 dias após TLCA. RESULTADOS: GluChon reduziu significativamente a dor articular nesse modelo (p<0...

Determinação da estrutura cristalográfica da enzima da Glucosamina-6-fosfato desaminase de E.coli K12 e seus complexos com ativador alostérico e inibidor; Crystal structure of enzyme glucosamine-6-phosphate deaminase de E. coli K12 and its complexes with allosteric activator and inhibitor

Fontes, Marcos Roberto de Mattos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 07/08/1995 PT
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A enzima Glucosamina-6-fosfato desaminase (GlcN6P desaminase) é envolvida na conversão reversível da D-glucosamina-6-fosfato (GlcN6P) em Fru6P e amônia, como parte do caminho metabólico de aminoaçúcares como fonte de energia celular. A enzima hexamérica (peso mol. 178200) exibe uma cooperatividade homotrópica intensa em direção à GlcN6P a qual é modulada alostericamente pelo ativador N-acetil-D-glucosamina 6-fosfato (GlcNAc6P). A GlcN6P desaminase foi cristalizada no grupo espacial R32, com parâmetros de rede a = b = 125.9 Å e c = 223.2 Å e um conjunto de dados à 2.1 Å de resolução foi coletado usando radiação de luz síncrotron (Horjales et ai., 1992). A procura no banco de dados de seqüências OWL não mostrou homologia significante com qualquer outra família de proteína, desta maneira a determinação da estrutura foi feita pela técnica de substituição isomórfica múltipla (MIR) a partir de dois derivados, um composto de platina, o K2PtCl4 e um complexo de mercúrio, o ácido mersálico. O mapa MIR a 3 Å de resolução mostrou contornos claros e utilizando técnicas de nivelamento de solvente (solvent flattening) estendeu-se as fases até 2.5 Å. A enzima cristaliza-se com dois monômeros na unidade assimétrica. A densidade eletrônica final foi interpretada com o auxílio do programa gráfico 'O'...

Comparação do efeito condroprotetor terapêutico da glicosamina em relação à diacereína no modelo experimental de artrose em ratos; A comparison between the therapeutic chondroprotective effects of glucosamine and diacerhein in an experimental model of arthritis in rats

Lopes, Alex Silva Santiago
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 11/12/2006 PT
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O objetivo do presente estudo é o de comparar funcionalmente ehistologicamente o efeito terapêutico da glicosamina em relação a diacereína no modelo experimental de artrose em ratos. Trinta ratos Wistar foram submetidos a meniscectomia medial do joelho direito. Dez animais receberam 50mg/kg/dia de diacereína a partir do 3o até o 7o mês de pósoperatório (PO), dez animais receberam 40mg/kg/dia de glicosamina e dez animais não receberam nenhuma medicação. Todos foram sacrificados no 7o mês PO. Foram medidos os ângulos de extensão máxima de cada joelho. A análise histológica com hematoxilina-eosina e alcian blue foi feita de cada um dos côndilos tibiais e femorais. Todos os joelhos operados apresentaram amplitude de extensão do joelho mais limitada que o lado contra-lateral (p=0,001), porém os que receberam diacereína apresentavam menor rigidez (p=0,005). Histologicamente, o modelo experimental levou a uma artrose de leve a moderada estatisticamente diferente do joelho contra-lateral não operado (p=0,001). Já os joelhos dos ratos que fizeram uso das medicações não apresentaram diferenças significativas (p=0,3) entre aqueles que tomaram glicosamina e os que fizeram uso da diacereína. As diferenças entre os joelhos operados medicados e não medicados também não foram estaticamente diferentes. Conclusões: O uso terapêutico da diacereína e glicosamina diminuiu a rigidez articular e retardou a progressão da artrose. A diacereína atuou melhor na mobilidade articular do que a glicosamina...

Structure and catalytic mechanism of glucosamine 6-phosphate deaminase from Escherichia coli at 2.1 Å resolution

Oliva, Glaucius; Fontes, Marcos R.M.; Garratt, Richard C.; Altamirano, Myriam M.; Calcagno, Mario L.; Horjales, Eduardo
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 1323-1332
ENG
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Background: Glucosamine 6-phosphate deaminase from Escherichia coli is an allosteric hexameric enzyme which catalyzes the reversible conversion of D-glucosamine 6-phosphate into D-fructose 6-phosphate and ammonium ion and is activated by N-acetyl-D-glucosamine 6-phosphate. Mechanistically, it belongs to the group of aldose-ketose isomerases, but its reaction also accomplishes a simultaneous amination/deamination. The determination of the structure of this protein provides fundamental knowledge for understanding its mode of action and the nature of allosteric conformational changes that regulate its function. Results: The crystal structure of glucosamine 6-phosphate deaminase with bound phosphate ions is presented at 2.1 Å resolution together with the refined structures of the enzyme in complexes with its allosteric activator and with a competitive inhibitor. The protein fold can be described as a modified NAD-binding domain. Conclusions: From the similarities between the three presented structures, it is concluded that these represent the enzymatically active R state conformer. A mechanism for the deaminase reaction is proposed. It comprises steps to open the pyranose ring of the substrate and a sequence of general base-catalyzed reactions to bring about isomerization and deamination...

Participation of N-acetyl-D-glucosamine carbohydrate moieties in the recognition of Schistosoma mansoni sporocysts by haemocytes of Biomphalaria tenagophila

Martins-Souza,Raquel Lopes; Pereira,Cintia Aparecida Jesus; Rodrigues,Leonardo; Araújo,Emília Souza; Coelho,Paulo Marcos Zech; Corrêa Jr,Ary; Negrão-Corrêa,Deborah
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2011 EN
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Lectin-carbohydrate binding may be involved in the recognition of Schistosoma mansoni sporocysts by haemocytes of Biomphalaria; therefore, we tested if this interaction is associated with snail resistance against Schistosoma infection. In vitro data showed that most of the S. mansoni sporocysts cultured with haemocytes from Biomphalaria glabrata BH, a highly susceptible snail strain, had a low number of cells that adhered to their tegument and a low mortality rate. Moreover, the addition of N-acetyl-D-glucosamine (GlcNAc) did not alter this pattern of adherence and mortality. Using haemocytes and haemolymph of Biomphalaria tenagophila Cabo Frio, we observed a high percentage of sporocysts with adherent cells, but complete encapsulation was not detected. Low concentrations of GlcNAc increased haemocyte binding to the sporocysts and mortality, which returned to basal levels with high concentrations of the carbohydrate. In contrast, haemocytes plus haemolymph from B. tenagophila Taim encapsulated cellular adhesion index of level 3 and destroyed over 30% of the S. mansoni sporocysts in culture. Interestingly, the addition of GlcNAc, but not mannose, to the culture medium resulted in the significant inhibition of cellular adhesion to the parasite tegument and the reduction of parasite mortality...

Use of glucosamine and chondroitin to treat osteoarthritis: a review of the literature

Lopes Junior,Osmar Valadao; Inacio,Andre Manoel
Fonte: Sociedade Brasileira de Ortopedia e Traumatologia Publicador: Sociedade Brasileira de Ortopedia e Traumatologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2013 EN
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To evaluate the current evidence that support or disprove the use of glucosamine and chondroitin in the treatment of patients with osteoarthritis. We performed a literature review using the databases of Medline, PubMed and the Cochrane Controlled Trial Register and Cochrane Databases Systematic Reviews (Cochrane Library).We considered only studies with high level of evidence.The study included analysis of randomized controlled trials that included at least 100 patients in each intervention group, meta-analyzes and systematic reviews. Seven meta-analysis, one systematic review and five randomized clinical trials fit inclusion criteria of this review. Considering the best evidences until now, the use of glucosamine and chondroitin does not provide clinical relevant benefits to patients with osteoarthritis of the knee or hip (Level I of evidence and grade A of recommendation). Further trials with adequate technology are necessaries to elucidate this question.

Design, synthesis and In Vitro evaluation on glucosamine-6P synthase of aromatic analogs of 2-Aminohexitols-6P

Dias,Danielle F.; Roux,Céline; Durand,Philippe; Iorga,Bogdan; Badet-Denisot,Marie A.; Badet,Bernard; Alves,Ricardo J.
Fonte: Sociedade Brasileira de Química Publicador: Sociedade Brasileira de Química
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2010 EN
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The aminosugars are very important structural components of bacterial and fungi cell walls. Glucosamine-6-phosphate synthase (GlmS), which catalyses the first step of the aminosugar biosynthetic pathway i.e. the formation of D-glucosamine-6-phosphate from D-fructose-6-phosphate, is therefore an interesting target in the fight against microorganisms. In this work is described the synthesis of aromatic analogs of 2-amino-2-deoxy-D-glucitol-6-phosphate (ADGP) and its epimer 2-amino-2-deoxy-D-manitol-6-phosphate (ADMP), two important inhibitors of GlmS. The aromatic analogs displayed modest inhibitory activity against GlmS, with IC50 in the mmol L-1 range.

Diacerhein versus glucosamine in a rat model of osteoarthritis

Rezende,Márcia Uchôa de; Gurgel,Henrique Melo de Campos; Vilaça Junior,Paulo Roberto; Kuroba,Rogério Kawassaki; Lopes,Alex Silva Santiago; Phillipi,Renée Zon; Hernandez,Arnaldo José
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2006 EN
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PURPOSE: The purpose of this study was to compare the chondroprotective effect of diacerein and glucosamine regarding degenerative changes and articular stiffness in an experimental model of arthritis. METHODS: Twenty rats underwent medial meniscectomy on the right knee. Ten animals were given diacerhein, and 10 were given glucosamine, from day 1 to the third month postoperatively, when all of them were killed. Histological and functional analysis of the knees were performed (measurement of maximum extension). RESULTS: All operated knees showed more limited extension values and more degenerative changes as compared to nonoperated contralateral sides. A comparison of the two drugs showed that the degree of articular stiffness was significantly lower with diacerein, although degenerative changes were similar. CONCLUSIONS: 1) Prophylactic use of diacerein leads to lower degree of articular stiffness when compared to glucosamine; 2) The prophylactic chondroprotective effects of diacerein and glucosamine are histologically similar.

Effects of glucosamine on the tibial epiphyseal disk of ovariectomized rats: morphologic and morphometric analysis

Maganhin,Carla C; Correa,Olga; Regina CT,Gomes; Simões,Ricardo; Baracat,Edmund C; Soares-Jr,José Maria
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2007 EN
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OBJECTIVE: The aim of this study was to analyze the effect of glucosamine sulfate on the tibial epiphyseal disk of the ovariectomized rats. METHODS: After ovariectomy (OVx), 28 female rats were randomly divided into 4 experimental groups with 7 animals each, treated as follows: OVx 21 - vehicle (NaCl 0.9%) 0.5 mL/day) for 21 days; OVx GS21 - 230 mg/kg/day glucosamine sulphate for 21 days; OVx 45 - treated with NaCl 0.9% as above for 45 days; and OVx - GS45230 mg/kg/day glucosamine sulphate for 45 days. Seven intact animals in the proestrous phase were used as controls (CG). Upon treatment completion, the animals were sacrificed and the left knee joint was dissected and prepared for histological analysis. RESULTS: The percentage of remaining cartilage in new bone of the CG; that found in THE OVx GS45 group was significantly less than that of the OVx 21, OVx GS21, and OVx 45 groups. The percentage of trabecular bone in proestrous animals was the highest. The OVx GS45 group showed higher values compared with the other ovariectomized groups. These results were paralleled by the findings regarding the cells of the proliferative zone, since the CG had the highest values, and the values of the OVx GS45 group were greater than those of the OVx 21...

Glucosamine May Reduce Pain in Individuals with Knee Osteoarthritis

Pierce, Jacqueline
Fonte: Quens University Publicador: Quens University
Tipo: Objeto de Aprendizagem Formato: 117714 bytes; application/pdf
EN
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Clinical Question: Does treatment with oral glucosamine reduce the progression of joint structure and symptoms in people with knee osteoarthritis? Clinical Bottom Line: The effectiveness of oral glucosamine in reducing the progression of joint space narrowing has not yet been definitively established due to the small numbers of long term clinical trials and weak statistical evidence. However, the evidence from these studies suggests that oral glucosamine is effective in reducing symptomatic pain in subjects with knee osteoarthritis.

Cumulus expansion and glucose utilisation by bovine cumulus-oocyte complexes during in vitro maturation: the influence of glucosamine and follicle-stimulating hormone

McDowall, M.; Gilchrist, R.; Thompson, J.
Fonte: Bio Scientifica Ltd Publicador: Bio Scientifica Ltd
Tipo: Artigo de Revista Científica
Publicado em //2004 EN
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37.21%
Glucose is an important metabolite and its presence during in vitro oocyte maturation (IVM) can have profound effects on the oocyte’s developmental capacity. We have demonstrated that glucose uptake increases over a 24 h IVM period, with most accounted for as L-lactate production. However, as maturation proceeds, L-lactate production remains constant, suggesting an alternative role for glucose metabolism. We hypothesised that in the latter stages of oocyte maturation, glucose not accounted for by L-lactate production is utilised for FSH-stimulated extracellular matrix (ECM) synthesis. To examine precursor utilisation for synthesis of ECM, bovine cumulus–oocyte complexes (COCs) were matured in ± FSH and/or glucosamine (an alternative substrate of matrix components). Measurements included COC diameters, glucose consumption and L-lactate production in spent media and [U-14C]glucose incorporation into ECM. FSH significantly stimulated both diameter and glucose consumption during 20–24 h maturation compared with unstimulated complexes, although co-incubation with glucosamine and FSH decreased total glucose consumption 1.7-fold compared with FSH alone (P < 0.05). Furthermore, there was a linear relationship between glucose and L-lactate metabolism in the presence of glucosamine...

The effect of glucosamine concentration on the development and sex ratio of bovine embryos

Kimura, K.; Iwata, H.; Thompson, J.
Fonte: Elsevier Science BV Publicador: Elsevier Science BV
Tipo: Artigo de Revista Científica
Publicado em //2008 EN
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Glucosamine is a component of hyaluronic acid and an alternative substrate to glucose for the extracellular matrix synthesis of COCs. Its addition to an IVM medium reduces the glucose consumption of bovine COCs. Glucosamine is also metabolized to UDP-N-acetyl glucosamine (UDP-GlcNAc) via the hexosamine biosynthesis pathway and is utilized for O-linked glycosylation by the X-linked enzyme, O-linked GlcNAc transferase (OGT). Moreover, the inactivation of the second X chromosome in female embryos is influential in producing the sex ratio bias observed in vitro when embryos are cultured in the presence of glucose above 2.5mM. Accordingly, the aim of this study is to examine whether the presence of glucosamine during maturation or embryo culture causes a sex ratio bias in bovine blastocysts. Glucosamine was added to the medium in three different embryo developmental periods: in vitro maturation, the one-cell to eight-cell stage (before the maternal-zygotic transition, MZT), and the eight-cell to blastocyst stage (after MZT). When glucosamine was added during in vitro maturation, the developmental competence of oocytes was severely compromised. However, the sex ratio of embryos was not influenced. When glucosamine was added to embryo culture medium during development from one-cell to eight-cell stage (before MZT)...

Difficulties with assessing the benefit of glucosamine sulphate as a treatment for osteoarthritis

Burdett, N.; McNeil, J.
Fonte: Blackwell Publishing Asia Publicador: Blackwell Publishing Asia
Tipo: Artigo de Revista Científica
Publicado em //2012 EN
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Osteoarthritis is a chronic disease with a major impact on quality of life for a large proportion of the population. It is a disease for which to date there has been no disease-modifying therapy identified. As a result of its physiological role in articular cartilage, glucosamine sulphate has been postulated as a treatment for osteoarthritis. Claims have included symptomatic relief and even reduction in the rate of disease progression. Despite promising in vitro studies, however, the role of glucosamine sulphate in the management of osteoarthritis remains unclear. Studies addressing this issue have generated a wide range of conclusions, and these are discussed here. Methodological issues need to be addressed in order to gauge whether there is true benefit. On current evidence, it would appear that the benefits of dietary supplementation with glucosamine sulphate are limited to mild symptomatic relief, while a disease-modifying agent for this disease remains elusive.; Nikki Burdett and Julian David McNeil

Use of chondroitin sulphate and glucosamine sulphate in degenerative changes in TMJ: a systematic review

Machado,Eduardo; Machado,Patricia; Cunali,Paulo Afonso
Fonte: Dental Press International Publicador: Dental Press International
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2012 EN
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INTRODUCTION: Degenerative changes in Temporomandibular Joint (TMJ) have increased in prevalence and severity over the years. Within this context, it's necessary to obtain safe and effective therapies for control and management of the patient in cases of osteoarthritis and osteoarthrosis of the TMJ. Therapeutic options range from intra-articular infiltration protocols, occlusal splints, pharmacological therapies and physiotherapy and educational measures. The alternative treatment with structure-modifying agents, like as chondroitin and glucosamine sulphates, showed promising results, and especially safety. Thus, through a systematic literature review, this study aimed to analyze and discuss effectiveness and safety of chondroitin and glucosamine in degenerative changes of the TMJ. METHODS: Survey in research bases MEDLINE, Cochrane, EMBASE, Pubmed, Lilacs and BBO, between the years of 1966 and January 2009, with focus in randomized clinical trial (RCTs) and quasi-randomized clinical trials, systematic reviews and meta-analysis. RESULTS: After application of the inclusion criteria 2 articles were selected, both randomized controlled double-blind clinical trials, which evaluated the effectiveness of chondroitin and glucosamine in degenerative changes of the TMJ. CONCLUSIONS: There is the necessity of further RCT...

Prescribing patterns of glucosamine in an older population: a national cohort study

Galvin, Rose; Cousins, Gráinne; Boland, Fiona; Motterlini, Nicola; Bennett, Kathleen; Fahey, Tom
Fonte: BioMed Central Publicador: BioMed Central
Tipo: info:eu-repo/semantics/article; all_ul_research; ul_published_reviewed
ENG
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peer-reviewed; Background: Glucosamine is commonly prescribed as a disease modulating agent in osteoarthritis. However, the evidence to date suggests that it has a limited impact on the clinical symptoms of the disease including joint pain, radiological progression, function and quality of life. The aim of this study was to examine the prescribing patterns of glucosamine from 2002–2011 in an elderly Irish national population cohort using data from the Health Service Executive Primary Care Reimbursement (HSE-PCRS) General medical services (GMS) Scheme. Methods: Patients aged ≥ 70 years on the HSE-PCRS pharmacy claims database between January 2002 and December 2011 were included. ATC code M01AX05 (glucosamine) was extracted. Prevalence rates per 1000 eligible population with 95% confidence intervals were calculated for all years and age groups (70–74 years, ≥75 years). A negative binomial regression analysis was used to determine longitudinal usage trends and compare prevalence rates across years, sex and age groups. Results: The annual patient rate of glucosamine prescribing increased significantly from 13.0/1000 eligible population (95% CI 12.6-13.4) in 2002 to 68.7/1000 population (95% CI 67.8-69.5) in 2009 before decreasing to 62.4/1000 population (95% CI 61.6-63.2) in 2011. The rate of prescribing of glucosamine varied with sex...

Diacereína x glicosamina no modelo de artrose em ratos; Diacerhein versus glucosamine in a rat model of osteoarthritis

Rezende, Márcia Uchôa de; Gurgel, Henrique Melo de Campos; Vilaça Junior, Paulo Roberto; Kuroba, Rogério Kawassaki; Lopes, Alex Silva Santiago; Phillipi, Renée Zon; Hernandez, Arnaldo José
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/10/2006 ENG
Relevância na Pesquisa
37.12%
OBJETIVO: O trabalho foi realizado com o objetivo de comparar o efeito condroprotetor da diacereína em relação ao da glicosamina quanto às alterações degenerativas e à rigidez articular num modelo experimental de artrose. MÉTODOS: Vinte ratos foram submetidos à meniscectomia medial do joelho direito. Dez animais receberam diacereína, e dez glicosamina, todos do primeiro dia ao terceiro mês pós-operatório, quando foram sacrificados. Foram realizadas análise histológica e funcional (medida da extensão máxima) dos joelhos. RESULTADOS: Todos os joelhos operados apresentaram amplitude de extensão mais limitada e maiores alterações degenerativas, em relação ao lado contra-lateral não operado. Ao compararmos as duas drogas, a rigidez articular foi significantemente menor com a diacereína, e as alterações degenerativas foram semelhantes. CONCLUSÕES: 1- O uso profilático da diacereína leva à menor rigidez articular em relação a glicosamina. 2- O efeito condroprotetor profilático da diacereína é semelhante, histologicamente, ao da glicosamina.; PURPOSE: The purpose of this study was to compare the chondroprotective effect of diacerein and glucosamine regarding degenerative changes and articular stiffness in an experimental model of arthritis. METHODS: Twenty rats underwent medial meniscectomy on the right knee. Ten animals were given diacerhein...

Efeitos da terapia de reposição hormonal estroprogestativa sobre o sistema de coagulação e de fibrinólise em mulheres na pós-menopausa; Effects of glucosamine on the tibial epiphyseal disk of ovariectomized rats: morphologic and morphometric analysis

Maganhin, Carla C; Correa, Olga; Regina CT, Gomes; Simões, Ricardo; Baracat, Edmund C; Soares-Jr, José Maria
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/2007 ENG
Relevância na Pesquisa
37.12%
OBJETIVO: O alvo deste estudo foi analisar o efeito do sulfato de glicosamina no disco epifisário da tíbia em ratas ooforectomizadas. MÉTODOS: Após a ooforectomia (OVx), 28 ratas foram aleatoriamente divididas em 4 grupos experimentais de 7 animais cada, tratados da seguinte maneira: OVx 21 - veículo (0,5ml de NaCl 0.9% ip uma vez ao dia) por 21 dias; OVx GS21 230 - mg/kg peso corporal por dia de sulfato de glicosamina, 21 dias; OVx 45 - tratados com NaCl 0.9% igual ao grupo OVx 21, por 45 dias; e OVx GS45 - 230 mg/kg peso corporal por dia com sulfato de glicosamina, 45 dias. Sete animais intactos, na fase de proestro, foram usados como controle (CG). Ao completar o tratamento, os animais foram sacrificados e a articulação do joelho esquerdo foi dissecada e preparada para análise histológica. RESULTADOS: A porcentagem de cartilagem remanescente no novo osso do CG foi a menor. Os achados no grupo OVx GS45 foi significantemente menor do que no grupo OVx 21, OVx GS21 e OVx 45. A porcentagem de osso trabecular nos animais em pró-estro foi a maior. O grupo OVx GS45 mostrou valores maiores em relação aos outros grupos ooforectomizados. Esses resultados foram correspondentes aos achados em relação às células da zona proliferativa...