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Caracterização da diversidade genética, da estrutura populacional e do parentesco de arara-azul-grande (Anodorhynchus hyacintthinus) por meio da análise dos genomas nuclear e mitocondrial; Characterization of the genetic diversity, population genetic structure and relatedness of hyacinth macaw (Anodorhynchus hyacinthinus) based on microsatellite and mitochondrial DNA

Presti, Flavia Torres
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 27/01/2011 PT
Relevância na Pesquisa
36.28%
O Brasil é o país mais rico do mundo em espécies de psitacídeos (cerca de 74), sendo 17 delas ameaçadas de extinção. Entre elas está a arara-azul (Anodorhynchus hyacinthinus) que é considerada vulnerável e pode se tornar ameaçada num futuro próximo, em conseqüência do intenso tráfico ilegal e perda do seu habitat. No presente estudo estimamos os níveis de variabilidade e caracterizamos a estrutura genética de populações naturais de A. hyacinthinus. Analisamos 10 locos de microssatélites de 98 indivíduos e seqüências concatenadas de genes mitocondriais (ND5, citocromo-b e ND2; 2123 pb total) de 80 indivíduos. O índice de diversidade genética foi considerado baixo em relação a outras espécies de psitacídeos. Além disso, os índices RST e a análise bayesiana dos dados de microssatélites indicaram moderada estruturação genética entre indivíduos de quatro regiões geográficas (Pantanal norte, Pantanal sul, norte e nordeste), mas os índices de FST indicaram diferenciação somente entre três regiões (norte e nordeste sem diferenciação). A estruturação entre essas três regiões foi congruente com a forte estruturação genética apontada pelos índices de FST e pela rede de haplótipos das seqüências mitocondriais. Baseado nos dados mitocondriais o tempo de divergência entre os grupos genéticos de A. hyacinthinus foi estimado em 16 a 42 mil anos atrás...

Development of a real-time PCR method for rapid sexing of human preimplantation embryos

Martinhago, C. D.; Vagnini, L. D.; Petersen, C. G.; Mauri, A. L.; Baruffi, R. L.; de Oliveira, R. M.; Franco, J. G.
Fonte: Reproductive Healthcare Ltd Publicador: Reproductive Healthcare Ltd
Tipo: Artigo de Revista Científica Formato: 75-82
ENG
Relevância na Pesquisa
36.59%
Genes on the X chromosome are known to be responsible for more than 200 hereditary diseases. After IVF, the simple selection of embryo sex before uterine transfer can prevent the occurrence of affected offspring among couples at risk for these genetic disorders. The aim of this investigation was to develop a rapid method of preimplantation genetic diagnosis (PGD) using real-time polymerase chain reaction (PCR) for the sexing of human embryos, and to compare it to the fluorescence in-situ hybridization technique, considered to be the gold standard. After biopsies were obtained from 40 surplus non-viable embryos for transfer, a total of 98 blastomeres were analysed. It was possible to analyse 24 embryos (60%) by both techniques, generating a total of 70 blastomeres (35 per technique), white 28 blastomeres from 16 embryos (40%) were analysed only by real-time PCR. A rapid and safe method was developed in the present study for the sexual diagnosis of a single human cell (blastomere and buccal cell) using the emerging technology of real-time PCR. (C) 2009, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Practical considerations of embryo manipulation: Preimplantation genetic typing

Garcia, J. F.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 1393-1399
ENG
Relevância na Pesquisa
36.24%
In the past years, research in embryo technologies is moving to the establishment of preimplantation genetic typing or also denominated preimplantation genetic diagnosis (PGD). The objectives of these tests are the prevention of genetic diseases transmission and the prediction of phenotypic characteristics, as well as sex determination, genetic disorders and productive and reproductive profiles, prior to the embryo transfer or freezing, during early stages of development. This paper points out the state-of-the-art of PGD, mainly in cattle and discuss the perspectives of multiloci genetic analysis of embryos. (C) 2001 by Elsevier B.V.

ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya

Costa,Fabiane Rabelo da; Pereira,Telma Nair Santana; Gabriel,Ana Paula Candido; Pereira,Messias Gonzaga
Fonte: Crop Breeding and Applied Biotechnology Publicador: Crop Breeding and Applied Biotechnology
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 EN
Relevância na Pesquisa
36.17%
ISSR markers are polymorphic and their results easily reproducible. They are therefore intensely used in phylogenetic studies and sex differentiation of some economically interesting plant species. The objectives of this study were to analyze the genetic diversity in Caricaceae using ISSR markers, to identify a specific ISSR band that could distinguish female from hermaphrodite papaya genotypes and to verify whether this marker could be used for early sex differentiation. The ISSR-PCR was performed with nine primers and they could distinguish all species. It was observed that Jacaratia spinosa was closer to Vasconcellea than to Carica. The species C. papaya was only distantly related to both genera. A 500 bp ISSR marker was found in 25 % of the papaya genotypes studied. Specifically in these cases this marker could be used for early sex differentiation in papaya.

Genetic elimination of dengue vector mosquitoes

Wise de Valdez, Megan R.; Nimmo, Derric; Betz, John; Gong, Hong-Fei; James, Anthony A.; Alphey, Luke; Black, William C.
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
36.12%
An approach based on mosquitoes carrying a conditional dominant lethal gene (release of insects carrying a dominant lethal, RIDL) is being developed to control the transmission of dengue viruses by vector population suppression. A transgenic strain, designated OX3604C, of the major dengue vector, Aedes aegypti, was engineered to have a repressible female-specific flightless phenotype. This strain circumvents the need for radiation-induced sterilization, allows genetic sexing resulting in male-only releases, and permits the release of eggs instead of adult mosquitoes. OX3604C males introduced weekly into large laboratory cages containing stable target mosquito populations at initial ratios of 8.5–10∶1 OX3604C∶target eliminated the populations within 10–20 weeks. These data support the further testing of this strain in contained or confined field trials to evaluate mating competitiveness and environmental and other effects. Successful completion of the field trials should facilitate incorporation of this approach into area-wide dengue control or elimination efforts as a component of an integrated vector management strategy.

A genetic polymorphism in the sex-linked ATP5A1 gene is associated with individual fitness in Ovenbirds (Seiurus aurocapilla)

Toms, Judith D; Eggert, Lori S; Arendt, Wayne J; Faaborg, John
Fonte: Blackwell Publishing Ltd Publicador: Blackwell Publishing Ltd
Tipo: Artigo de Revista Científica
Publicado em /06/2012 EN
Relevância na Pesquisa
36.17%
While testing genetic sexing techniques in Ovenbirds (Seiurus aurocapilla), we found a genetic polymorphism in the ATP5A1 gene in 38% of individuals. The Z′ allele included changes in both intronic and exonic portions of the sequenced region, but there was no evidence that this changed the resulting ATP synthase product. Males that had one or more copies of this allele had higher relative body mass (mass corrected for size) than other genotypes. This allele was unrelated to stable isotope signatures, and so was not a useful predictor of latitude within the eastern portion of the Ovenbird breeding range. Future studies are needed to determine whether this polymorphism may be a useful geographic marker. This study is the first to link polymorphisms in the sex-linked ATP5A1 gene with fitness effects.

Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori

Tan, Anjiang; Fu, Guoliang; Jin, Li; Guo, Qiuhong; Li, Zhiqian; Niu, Baolong; Meng, Zhiqi; Morrison, Neil I.; Alphey, Luke; Huang, Yongping
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.59%
Transgene-based genetic sexing methods are being developed for insects of agricultural and public health importance. Male-only rearing has long been sought in sericulture because males show superior economic characteristics, such as better fitness, lower food consumption, and higher silk yield. Here we report the establishment of a transgene-based genetic sexing system for the silkworm, Bombyx mori. We developed a construct in which a positive feedback loop regulated by sex-specific alternative splicing leads to high-level expression of the tetracycline-repressible transactivator in females only. Transgenic animals show female-specific lethality during embryonic and early larval stages, leading to male-only cocoons. This transgene-based female-specific lethal system not only has wide application in sericulture, but also has great potential in lepidopteran pest control.

Australian endemic pest tephritids: genetic, molecular and microbial tools for improved Sterile Insect Technique

Raphael, Kathryn A; Shearman, Deborah CA; Gilchrist, A Stuart; Sved, John A; Morrow, Jennifer L; Sherwin, William B; Riegler, Markus; Frommer, Marianne
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 01/12/2014 EN
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36.2%
Among Australian endemic tephritid fruit flies, the sibling species Bactrocera tryoni and Bactrocera neohumeralis have been serious horticultural pests since the introduction of horticulture in the nineteenth century. More recently, Bactrocera jarvisi has also been declared a pest in northern Australia. After several decades of genetic research there is now a range of classical and molecular genetic tools that can be used to develop improved Sterile Insect Technique (SIT) strains for control of these pests. Four-way crossing strategies have the potential to overcome the problem of inbreeding in mass-reared strains of B. tryoni. The ability to produce hybrids between B. tryoni and the other two species in the laboratory has proved useful for the development of genetically marked strains. The identification of Y-chromosome markers in B. jarvisi means that male and female embryos can be distinguished in any strain that carries a B. jarvisi Y chromosome. This has enabled the study of homologues of the sex-determination genes during development of B jarvisi and B. tryoni, which is necessary for the generation of genetic-sexing strains. Germ-line transformation has been established and a draft genome sequence for B. tryoni released. Transcriptomes from various species...

Development of a Genetic Sexing System for Anopheles albimanus

Kaiser, P. E.; Seawright, J. A.; Dame, D. A.; Joslyn, D. J.
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica Formato: text/html
EN
Relevância na Pesquisa
46.45%
Several strains of a mosquito, Anopheles albimanus Wiedemann, were synthesized that allow the preferential elimination of females during any of the 4 life stages. This genetic sexing system utilizes propoxur susceptibility as a recessive conditional lethal, a T(Y;2R) translocation, and an In(2R) inversion. The propoxur resistance allele (dominant) was linked to the Y chromosome via a radiation-induced translocation and subsequent genetic recombination was suppressed by inversions. In one of the strains produced, 99.8% of the females were eliminated when treated with propoxur without male loss. Chemically sterilized males of one strain were found to be fully competitive for mating in a laboratory cage.

Genetic Sexing Systems in Anopheles arabiensis Patton (Diptera: Culicidae)

Lines, J. D.; Curtis, C. F.
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica Formato: text/html
EN
Relevância na Pesquisa
36.12%
Irradiation of male Anopheles arabiensis Patton, followed by crossing and selection, produced a stock in which both malathion and dieldrin resistance were linked closely to the male sex. Testis squashes revealed that a translocation had been induced between one of the autosomes and the Y chromosome. Translocations of this kind could be used to allow elimination of females from batches of males being prepared for a genetic control program. However, of the two resistances which have been translocated only dieldrin could in practice be used for sex separation, because this is expressed in the larval as well as the adult stage, whereas malathion resistance was found only in the adult stage. An attempt to produce a similar sex separation system for A. stephensi Liston was unsuccessful despite the screening of 712 progeny of irradiated males.

Gen??mica aplicada ?? reprodu????o equina; Gen??mica aplicada ?? reprodu????o equina

LEON, Priscila Marques Moura de
Fonte: Universidade Federal de Pelotas; Biotecnologia; Programa de P??s-Gradua????o em Biotecnologia; UFPel; BR Publicador: Universidade Federal de Pelotas; Biotecnologia; Programa de P??s-Gradua????o em Biotecnologia; UFPel; BR
Tipo: Tese de Doutorado Formato: application/pdf
POR
Relevância na Pesquisa
26.45%
In equine, the intersections between reproduction and genomics are numerous, however, little known about the genetic factors that acting on fertility. The conclusion of equine genome sequencing project, brings the oportunity to evaluate candidate genes and molecular biomarkers for specific reproductive characteristics. Based on this information, this PhD thesis aimed to develop genomic studies applied to equine reproduction. The first paper analyzed the expression of apoptotic-related genes (Bax, Bcl-2, survivin and p53) in equine cumulus-oocyte complex by qRT-PCR, comparing gene expression between morphologically distinct oocytes and cumulus cells during in vitro maturation. Results showed that survivin mRNA levels were higher (P<0.05) and p53 mRNA levels was lower (P<0.01) in oocytes compared to cumulus cells in morphologically healthy. On the other hand, expression of the Bax was significantly higher in morphologically healthy cumulus cells (P<0.02). The second paper analyzed a single nucleotide polymorphism (SNP) in the p53 gene, looking for associations with reproductive parameters in Thoroughbred mares. This is the first study demonstrating the Arginine/Proline SNP in equine exon 4 p53 gene. The heterozygous Arginine/Proline was found in 73.3% of Thoroughbreds compared to the homozygous Arg/Arg and Pro/Pro that was detected in 17.1% and 9.6% of mares...

Optimization of microsatellite genotyping and genetic sexing of non-invasively collected Polar Bear (Ursus maritimus) hair samples.

Harris, Christopher Matthew
Fonte: Quens University Publicador: Quens University
Tipo: Tese de Doutorado Formato: 794624 bytes; application/msword
EN
Relevância na Pesquisa
46.39%
Management of Polar Bears in Canada is based on population viability analysis done on data from aerial capture-mark-recapture survey data. These surveys are completed every 12-15 years, however with growing concern about declining numbers, and impact of global warming on habitat, there is interest in monitoring population status more often. This study investigates the utility of genotyping and genetic sexing of polar bear hairs as part of a bear activity survey which includes Inuit estimates of bear sex, age, and size estimates from tracks and the utility of multivariate analyses of digital images from footprints. From 7 sampling stations comprising cordons of barbed wire erected on the sea ice off northern King William Island in May 2006, samples of hair were collected from 79 barbs in 9 days. Most of these samples comprised 1-4 hairs, with the numbers of barbs involved in polar bear contacts ranging from 1-16. Eleven of 15 microsatellite loci worked well on hair samples with useful data even collected from single hairs. The genotyping error in using these methods was 5% or less across all loci – similar to that expected by chance. Twenty consensus genotypes were generated comprising 18 unique individuals. This was two less than the estimate of contacts provided by the Inuit participants based on tracks alone. Management implications and improvements to future studies are discussed.; NSERC

An Evaluation of Non-Invasive Ursus maritimus (Polar Bear) Sampling Stations: Is the Sampling Method Sex Biased?

Van Zuiden, Thomas
Fonte: Quens University Publicador: Quens University
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
36.39%
The current status of polar bear (Ursus maritimus) populations around the globe is generally unknown. Because polar bears are listed as ‘threatened’ under the endangered species act, there has been a large research effort towards learning more about this species in order to help conserve them. Until recently, all data gathered on polar bears was determined by aerial Capture, Mark, Recapture (CMR) surveys, usually performed every 12-15 years. This study attempts to investigate potential sex biases gathered by non-invasive sampling stations that allow for monitoring of polar bears over shorter time intervals. Through the genetic sexing of polar bear hairs using ZFX and SRY, genes 12 unique males and 19 unique females were resolved from 179 hair snags collected over three weeks from 66 sampling stations in May 2009. This sex ratio was not significantly different from a 50:50 sex ratio nor a sex ratio derived from an aerial Capture, Mark, Recapture survey of the same region during 1998-2000 (Taylor et al., 2006; χ2, P>0.05). The genetic sex ratio for these samples was significantly different from the sex ratio estimate derived from Inuit diagnoses of bear tracks from the same area and time of year ( χ2, P<0.05). The implications of these findings are discussed.; Dr. Boag's NSERC

Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient; Validação comparativa utilizando PCR quantitativo em tempo real (qPCR) e PCR convencional de sêmen bovino centrifugado em gradiente de densidade contínuo

RESENDE, M.V.; LUCIO, A.C.; PERINI, A.P.; OLIVEIRA, L.Z.; ALMEIDA, A.O.; ALVES, B.C.A.; MOREIRA-FILHO, C.A.; SANTOS, I.W.; HOSSEPIAN DE LIMA, V.F.M.
Fonte: Universidade Federal de Minas Gerais, Escola de Veterinária Publicador: Universidade Federal de Minas Gerais, Escola de Veterinária
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
26.44%
The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P<0.05). The percentage of female embryos in the Percoll and OptiPrep groups was 62.0% and 47.1%, respectively. These results were confirmed by qPCR of spermatozoa DNA and underestimation was seen only in the Percoll group. It was possible to sexing sperm using simple approach.; O objetivo do presente estudo foi determinar o enriquecimento de espermatozoides portadores do cromossomo X após a centrifugação em gradiente de densidade contínuo de Percoll ou OptiPrep, utilizando reação em cadeia da polimerase quantitativa em tempo real (qPCR) do DNA do espermatozoide e dos embriões bovinos produzidos in vitro resultantes pela PCR convencional. Espermatozoides descongelado foram depositados em gradientes de densidade e os tubos foram centrifugados. Os sobrenadantes foram gentilmente aspirados e os espermatozoides recuperados do fundo dos tubos. As taxas de clivagem e de blastocisto foram determinadas pela produção in vitro de embriões e a PCR foi realizada para a identificação genética do sexo dos embriões. Verificou-se diferença na taxa de blastocistos entre os grupos Percoll e OptiPrep (P<0...

Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient

Resende, M. V.; Lucio, A. C.; Perini, A. P.; Oliveira, L. Z.; Almeida, A. O.; Alves, B. C. A.; Moreira-Filho, C. A.; Santos, I. W.; Lima, V. F. M. Hossepian de
Fonte: Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária Publicador: Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
Tipo: Artigo de Revista Científica Formato: 544-551
ENG
Relevância na Pesquisa
26.44%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 04/06044-0; Processo FAPESP: 05/59357-9; O objetivo do presente estudo foi determinar o enriquecimento de espermatozoides portadores do cromossomo X após a centrifugação em gradiente de densidade contínuo de Percoll ou OptiPrep, utilizando reação em cadeia da polimerase quantitativa em tempo real (qPCR) do DNA do espermatozoide e dos embriões bovinos produzidos in vitro resultantes pela PCR convencional. Espermatozoides descongelado foram depositados em gradientes de densidade e os tubos foram centrifugados. Os sobrenadantes foram gentilmente aspirados e os espermatozoides recuperados do fundo dos tubos. As taxas de clivagem e de blastocisto foram determinadas pela produção in vitro de embriões e a PCR foi realizada para a identificação genética do sexo dos embriões. Verificou-se diferença na taxa de blastocistos entre os grupos Percoll e OptiPrep (P<0,05). A porcentagem de embriões de fêmeas nos grupos Percoll e OptiPrep foi de 62,0% e 47,1%, respectivamente. Estes resultados foram confirmados pela qPCR do DNA de espermatozoides e uma subestimação foi observada no grupo do gradiente de densidade de Percoll. Foi possível a sexagem de espermatozoides utilizando uma metodologia simples.; The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa...

A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina

Yan, Ying; Scott, Maxwell J.
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em 05/11/2015 EN
Relevância na Pesquisa
26.53%
Genetic approaches, including the sterile insect technique (SIT), have previously been considered for control of the Australian sheep blow fly Lucilia cuprina, a major pest of sheep. In an SIT program, females consume 50% of the diet but are ineffective as control agents and compete with females in the field for mating with sterile males, thereby decreasing the efficiency of the program. Consequently, transgenic sexing strains of L. cuprina were developed that produce 100% males when raised on diet that lacks tetracycline. However, as females die mostly at the pupal stage, rearing costs would not be significantly reduced. Here we report the development of transgenic embryonic sexing strains of L. cuprina. In these strains, the Lsbnk cellularization gene promoter drives high levels of expression of the tetracycline transactivator (tTA) in the early embryo. In the absence of tetracycline, tTA activates expression of the Lshid proapoptotic gene, leading to death of the embryo. Sex-specific RNA splicing of Lshid transcripts ensures that only female embryos die. Embryonic sexing strains were also made by combining the Lsbnk-tTA and tetO-Lshid components into a single gene construct, which will facilitate transfer of the technology to other major calliphorid livestock pests.

Evolution of mammalian sex chromosomes and sex determination genes: insights from monotremes.

Toledo-Flores, Deborah Fernanda
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2015
Relevância na Pesquisa
46.59%
Genetic sex determination systems are generally based on the presence of differentiated sex chromosomes. Birds have a ZZ/ZW sex chromosome system in which males are ZZ and females ZW, whereas mammals have an XX/XY system with males being XY and females XX. Monotremes have an extraordinary sex chromosome system that consists of multiple sex chromosomes: 5X5Y in platypus and 5X4Y in echidna. Intriguingly, the monotreme sex chromosomes show extensive homology to the bird ZW and not to the therian XY. However, sex determination in monotremes is still a mystery; the Y-specific Sry gene that triggers male sex determination in therian mammals is absent and so far very few genes have been identified on Y chromosomes in monotremes. To gain more insights into the gene content of Y-chromosomes and to identify potential sex determination genes in the platypus a collaborative large scale transcriptomic approach led to the identification of new male specific genes including the anti-Muellerian hormone AMH that I mapped to Y₅, this makes Amhy an exciting new candidate for sex determination in monotremes. Platypus chromosome 6 is largely homologous to the therian X and therefore it represents the therian proto sex chromosome. In addition, this autosome features a large heteromorphic nucleolus organizer region (NOR) and associates with the sex chromosomes during male meiosis (Casey and Daish personal communication). I investigated chromosome 6 heteromorphism in both sexes and found a number of sex-specific characteristics related to the extent of the NOR heteromorphism...

Identificação do sexo e variabilidade genética em uma população de Astronotus ocellatus (Agassiz, 1831) por marcadores ISSR

Paiva, Isadora Marques
Fonte: Universidade Federal de Lavras; Programa de Pós-Graduação em Ciências Veterinárias; UFLA; brasil; Departamento de Medicina Veterinária Publicador: Universidade Federal de Lavras; Programa de Pós-Graduação em Ciências Veterinárias; UFLA; brasil; Departamento de Medicina Veterinária
Tipo: Dissertação
Publicado em 17/12/2015 POR
Relevância na Pesquisa
26.75%
Studies conducted in order to investigate alternative methods for sexing fish species, such as Astronotus ocellatus, using reduced sexual dimorphism, are extremely important to facilitate reproduction management techniques. The objective of this study was to determine the feasibility of manual sexing, widely used by fish farmers and hobbyists, and the identification of specific molecular markers for a particular sex using ISSR markers. The latter technique also generates genetic diversity and similarity data, and is important for conservation studies. Manual sexing was performed by macroscopic analysis of the urogenital papilla. Fin and gonad samples of 30 A. ocellatus (±83,32g e ±15, 96 cm) were collected for DNA extraction and histology, respectively. For DNA extraction, we adopted the NaCl protocol (SAMBROOK, 1989). Quality samples were used for amplification, using universal ISSR primers with subsequent separation of the generated fragments by electrophoresis, and assessment of similarity and genetic diversity levels. The manual sexing did not appear as a viable technique to distinguish the sexes for this species, given the occurrence of 37% of errors during selection. Likewise...

Genética da conservação aplicada ao tráfico ilegal de aves; Conservation genetics applied to birds illegal trade

Gonçalves, BP; Wasko, AP
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ART.; Formato: application/pdf
Publicado em 26/12/2013 POR
Relevância na Pesquisa
26.59%
Birds represent the greater part of the animals associated to illegal trade/commerce in Brazil, specially due to some characteristics as song and feathers colors. Nowadays, genetic analyses comprehend one of the most effi cient approaches to generate data in order to solve and minimize the results of environmental crimes and illegal trade of wild animals. In birds, one of the genetic survey that can be used to subside conservation plans associated to illegal trade refers to molecular sexing, since it is not possible to identify the gender in some avian species based on morphological characters. The molecular sexing can be performed using DNA from different samples, as feathers and blood, and further amplifi cation of the CHD-Z e CHD-W (chromo helicaseDNA binding) gene regions. The sex-specifi c genetic profi les can support conservation programs of captive maintenance and/or reproduction and subsequent release or reintroduction of the animals on wild environment.; Entre os animais silvestres envolvidos em tráfico/comércio ilegal no Brasil, as aves compreendem um dos grupos mais atingidos, especialmente devido a características como canto e colorido das penas. Atualmente, análises genéticas compreendem uma das formas mais eficazes de gerar dados para solucionar e minimizar os resultados de crimes ambientais e comércio ilegal de animais silvestres. Uma das análises genéticas que podem ser utilizadas com o intuito de subsidiar programas conservacionistas associados ao tráfico ilegal de aves refere-se à sexagem molecular...

Sexing of in vitro produced bovine embryos: A review of methods with emphasis on PCR; DETERMINAÇÃO DO SEXO DE EMBRIÕES BOVINOS PRODUZIDOS IN VITRO: UMA REVISÃO DE MÉTODOS COM ÊNFASE PARA A PCR

RUFINO, F.A.; SENEDA, M.M.; ALFIERI, A.A.
Fonte: UFPR Publicador: UFPR
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Artigo Avaliado pelos Pares Formato: application/pdf
Publicado em 15/08/2006 POR
Relevância na Pesquisa
36.29%
The development of new animal reproduction biotechniques has been provided the obtaining of genetic improvement in cattle herds around the world. Recently, the ascension of the in vitro production (IVP) of bovine embryos provided important development in the technology of bovine embryos. Although being a consolidated technique, some studies show disproportion between the sexes of calves born by means of IVP, being the percentage of males higher than the theoretically expected rate of 50% for each sex. This inconvenient opened the perspective for the development of methods for sex determination for the embryos before the transference, once the production of females is desirable in economic terms. This revision will approach the advantages and disadvantages of the invasive and non-invasive methods developed for the sex determination in bovine embryos, with emphasis for PCR assay that is the technique that displays the best results.; O desenvolvimento de novas biotécnicas em reprodução animal tem proporcionado incrementos significativos no padrão genético de bovinos em todo o mundo. A produção in vitro (PIV) de embriões bovinos ocasionou um salto de qualidade na tecnologia da produção de embriões. Embora a PIV seja uma técnica consolidada...