Página 1 dos resultados de 364 itens digitais encontrados em 0.007 segundos

Potato cultivar identification using molecular markers

Rosa,Patricia Marchezi; Campos,Tatiana de; Sousa,Adna Cristina Barbosa de; Sforça,Danilo Augusto; Torres,Gisele Abigail Montan; Souza,Anete Pereira de
Fonte: Embrapa Informação Tecnológica; Pesquisa Agropecuária Brasileira Publicador: Embrapa Informação Tecnológica; Pesquisa Agropecuária Brasileira
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2010 EN
Relevância na Pesquisa
45.92%
The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.

Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems

Maluf,Mirian Perez; Silvestrini,Milene; Ruggiero,Luciana Machado de Campos; Guerreiro Filho,Oliveiro; Colombo,Carlos Augusto
Fonte: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" Publicador: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2005 EN
Relevância na Pesquisa
46.24%
One of the greatest problems in Coffea arabica breeding is identifying precisely any inbred line, based only on botanical and agronomical descriptors, because of the reduced genetic variability of the species, close pedigree origin, which results in small phenotypic variation. Recently, molecular markers have been used for plant germplasm characterization and identification in several commercial species. This work evaluates the reliability of three marker systems: RAPD, AFLP and SSR, to characterize the genetic variability of commercially-used Coffea inbred lines developed by the Instituto Agronômico (IAC), and their potential for cultivar identification. All methods identified polymorphisms among the cultivars. The genetic diversity recognized by the methods is very similar, although is very narrow. RAPD and SSR marker systems grouped more efficiently the evaluated cultivars according to parental origin. None of the methods allowed inbred line identification. Therefore for varietal protection, it would be necessary using a combination of botanical, agronomical and molecular markers descriptors for precise cultivar identification.

Varietal identification of coffee seeds by RAPD technique

Crochemore,Maria Lúcia; Nunes,Liliane Moreira; Andrade,Giselly Aparecida; Molinari,Hugo Bruno Correa; Vasconcellos,Maria Elizabeth
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2004 EN
Relevância na Pesquisa
45.92%
This study aimed the identification of cultivars and/or lines of Coffea arabica of commercial interest, using PCR-RAPD markers. The DNA of ground seeds lots of 12 cultivars and/or lines were evaluated with five primers (Operon OPA 01, OPA 04, OPG 11, OPY 16, and OPX 09) were obtained from a selection of 56 primers. The electrophoretic profiles allowed distinction among eight cultivars and/or lines as well as heterogeneity between and within lots of IAPAR59.

DNA fingerprinting of Japanese plum (Prunus salicina) cultivars based on microsatellite markers

Klabunde,Gustavo Henrique Ferrero; Dalbó,Marco Antonio; Nodari,Rubens Onofre
Fonte: Crop Breeding and Applied Biotechnology Publicador: Crop Breeding and Applied Biotechnology
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2014 EN
Relevância na Pesquisa
36.03%
Forty-seven Japanese plum (Prunus salicina) cultivars were genotyped with eight microsatellite markers, aiming at obtaining the DNA fingerprinting profiling, distinguishing and characterizing a representative set of Japanese plum cultivars. The eight SSR loci amplified 104 alleles (8 to 21 alleles per locus, mean 13). Polymorphism Information Content (PIC) ranged from 0.680 to 0.886 (mean 0.803). The observed heterozigozity (Ho) ranged from 0.529 to 0.915 (mean 0.770). Probability of Identity (I) of each locus ranged from 0.019 to 0.113 (mean 0.054). The combined Probability of Identity was 2.66 x 1011, and the Power of Exclusion of the eight loci was 99.99976%. 57 out of 104 alleles showed frequency lower than 0.05. These low allele frequencies contributed to raise the distinguish ability of plum cultivars. These results will contribute, as excellent descriptors, to select parental for crossings, to perform early identification of segregating clones with potential to be cultivars, and to protect the cultivars.

Rice Seed Cultivar Identification Using Near-Infrared Hyperspectral Imaging and Multivariate Data Analysis

Kong, Wenwen; Zhang, Chu; Liu, Fei; Nie, Pengcheng; He, Yong
Fonte: MDPI Publicador: MDPI
Tipo: Artigo de Revista Científica
Publicado em 12/07/2013 EN
Relevância na Pesquisa
46.03%
A near-infrared (NIR) hyperspectral imaging system was developed in this study. NIR hyperspectral imaging combined with multivariate data analysis was applied to identify rice seed cultivars. Spectral data was exacted from hyperspectral images. Along with Partial Least Squares Discriminant Analysis (PLS-DA), Soft Independent Modeling of Class Analogy (SIMCA), K-Nearest Neighbor Algorithm (KNN) and Support Vector Machine (SVM), a novel machine learning algorithm called Random Forest (RF) was applied in this study. Spectra from 1,039 nm to 1,612 nm were used as full spectra to build classification models. PLS-DA and KNN models obtained over 80% classification accuracy, and SIMCA, SVM and RF models obtained 100% classification accuracy in both the calibration and prediction set. Twelve optimal wavelengths were selected by weighted regression coefficients of the PLS-DA model. Based on optimal wavelengths, PLS-DA, KNN, SVM and RF models were built. All optimal wavelengths-based models (except PLS-DA) produced classification rates over 80%. The performances of full spectra-based models were better than optimal wavelengths-based models. The overall results indicated that hyperspectral imaging could be used for rice seed cultivar identification...

Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification* #

Jia, Hui-min; Shen, Yu-tong; Jiao, Yun; Wang, Guo-yun; Dong, Xiao; Jia, Hui-juan; Du, Fang; Liang, Sen-miao; Zhou, Chao-chao; Mao, Wei-hua; Gao, Zhong-shan
Fonte: Zhejiang University Press Publicador: Zhejiang University Press
Tipo: Artigo de Revista Científica
Publicado em /11/2014 EN
Relevância na Pesquisa
35.86%
Chinese bayberry (Myrica rubra Sieb. et Zucc.) is one of the important subtropical fruit crops native to the South of China and Asian countries. In this study, 107 novel simple sequence repeat (SSR) molecular markers, a powerful tool for genetic diversity studies, cultivar identification, and linkage map construction, were developed and characterized from whole genome shotgun sequences. M13 tailing for forward primers was applied as a simple method in different situations. In total, 828 alleles across 45 accessions were detected, with an average of 8 alleles per locus. The number of effective alleles ranged from 1.22 to 10.41 with an average of 4.08. The polymorphic information content (PIC) varied from 0.13 to 0.89, with an average of 0.63. Moreover, these markers could also be amplified in their related species Myrica cerifera (syn. Morella cerifera) and Myrica adenophora. Seventy-eight SSR markers can be used to produce a genetic map of a cross between ‘Biqi’ and ‘Dongkui’. A neighbor-joining (NJ) tree was constructed to assess the genetic relationships among accessions, and the elite accessions ‘Y2010-70’, ‘Y2012-140’, and ‘Y2012-145’, were characterized as potential new genotypes for cultivation.

Potato cultivar identification using molecular markers.

ROSA, P. M.; CAMPOS, T. de; SOUSA, A. C. B. de; SFORÇA, D. A.; TORRES, G. A. M.; SOUZA, A. P. de
Fonte: Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 1, p. 110-113, jan. 2010. Publicador: Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 1, p. 110-113, jan. 2010.
Tipo: Artigo em periódico indexado (ALICE)
EN
Relevância na Pesquisa
45.92%
Abstract ? The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.; 2010

Identification of earl millet cultivars using both microsatellites and enzymatic markers

Fonte: Ribeirão Preto Foundation for Research Publicador: Ribeirão Preto Foundation for Research
Tipo: Artigo de Revista Científica
EN_US
Relevância na Pesquisa
45.95%
The increasing number of protected and registered cultivars and problems involving seed commercialization make distinction and identification of cultivars imperative. Millet (Pennisetum glaucum), a crop species with protected cultivars in Brazil, has been the target of seed piracy. Thus, with the objective of identifying different lots with regard to origin, we characterized six cultivars of commercialized millet of proven origin by means of the electrophoretic patterns of the isoenzymes alcohol dehydrogenase, esterase and glutamate oxaloacetate transaminase and by microsatellite markers, using primers specific for millet. The six cultivars were separated with four microsatellite loci. Based on this characterization, certification of genetic purity was undertaken for public domain commercialized seed lots. The isoenzymatic markers were also tested for stability of the patterns. Esterase patterns were altered in seeds with different physiological quality and health conditions, but this alteration did not hinder identification of the cultivars. It was observed that most of the millet seed lots commercialized in Brazil as being in public domain belong to other cultivars.

Chimerism in grapevines: implications for cultivar identity, ancestry and genetic improvement

Franks, Tricia K.; Botta, R.; Thomas, M. R.; Franks, J.
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
Publicado em //2002 EN
Relevância na Pesquisa
35.92%
In the course of DNA profiling of grapevine cultivars using microsatellite loci we have occasionally observed more than two alleles at a locus in some individuals and have identified periclinal chimerism as the source of such anomalies. This phenomenon in long-lived clonally propagated crops, such as grapevine, which contains historically ancient cultivars, may have a role in clonal differences and affect cultivar identification and pedigree analysis. Here we show that when the two cell layers of a periclinal chimera, Pinot Meunier, are separated by passage through somatic embryogenesis the regenerated plants not only have distinct DNA profiles which are different from those of the parent plant but also have novel phenotypes. Recovery of these phenotypes indicates that additional genetic differences can exist between the two cell layers and that the Pinot Meunier phenotype is due to the interaction of genetically distinct cell layers. It appears that grapevine chimerism can not only modify phenotype but can also impact on grapevine improvement as both genetic transformation and conventional breeding strategies separate mutations in the L1 and L2 cell layers.; T. Franks, R. Botta, M. R. Thomas, J. Franks; The original publication can be found at www.springerlink.com

Crowdsourcing information system for camellia cultivar identification; Sistema colaborativo para a identificação de cultivares de camélia

Ventura, André Fidalgo
Fonte: Universidade de Aveiro Publicador: Universidade de Aveiro
Tipo: Dissertação de Mestrado
ENG
Relevância na Pesquisa
56.41%
Camellia cultivar identification is difficult due to the lack of complete, systematic information about the more than 20,000 registered cultivars, and cannot be achieved through disjointed individual efforts. This dissertation describes a collaborative platform for camellia cultivar identification harnessing the capabilities of modern information and communication technologies. The strategy proposed is based on the crowdsourcing concept. The entries on this system originate in cultivar identification requests submitted online. The system is equipped with a register of cultivar names. Through cultivar identification quizzes presented online, it gradually collects responses to the requests. Simultaneously, based on the performance of quiz respondents, the system applies metrics to establish their reputation and weigh their answers accordingly to calculate the probability of a specimen belonging to a given cultivar. User requests can be answered with a quantifiable degree of certainty according to the number of answers, their agreement and the reputation of each respondent.; A identificação de cultivares de camélia é difícil devido à falta de informação completa e sistemática sobre os mais de 20 000 cultivares registados, e não pode ser alcançada através de esforços individuais desconexos. Esta dissertação descreve uma plataforma colaborativa para a identificação de cultivares de camélia...

Optimal use of SSR markers for varietal identification of upland cotton

Pereira,Guilherme da Silva; Cazé,Ana Luíza Ramos; Silva,Michelle Garcia da; Almeida,Vanessa Cavalcante; Magalhães,Fernanda Oliveira da Cunha; Silva Filho,João Luís da; Barroso,Paulo Augusto Vianna; Hoffmann,Lúcia Vieira
Fonte: Embrapa Informação Tecnológica; Pesquisa Agropecuária Brasileira Publicador: Embrapa Informação Tecnológica; Pesquisa Agropecuária Brasileira
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/07/2015 EN
Relevância na Pesquisa
35.95%
Abstract: The objective of this work was to identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the varieties. Initially, 92 SSR markers were genotyped in 20 Brazilian cotton cultivars. Of this total, 38 loci were polymorphic, two of which were amplified by one primer pair; the mean number of alleles per locus was 2.2. The values of polymorphic information content (PIC) and discrimination power (DP) were, on average, 0.374 and 0.433, respectively. The mean genetic distance was 0.397 (minimum of 0.092 and maximum of 0.641). A panel of 96 varieties originating from different regions of the world was assessed by 21 polymorphic loci derived from 17 selected primer pairs. Among these varieties, the mean genetic distance was 0.387 (minimum of 0 and maximum of 0.786). The dendrograms generated by the unweighted pair group method with arithmetic average (UPGMA) did not reflect the regions of Brazil (20 genotypes) or around the world (96 genotypes), where the varieties or lines were selected. Bootstrap resampling shows that genotype identification is viable with 19 loci. The polymorphic markers evaluated are useful to perform varietal identification in a large panel of cotton varieties and may be applied in studies of the species diversity.

Almond improvement via micropropagation, cryopreservation, and s-allele identification.

Channuntapipat, Chockpisit
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2002
Relevância na Pesquisa
36.09%
The Australian almond improvement program was initiated in 1997 to develop improved cultivars that are adapted to local conditions and consumer demands. The program combines molecular techniques along with the traditional approach of controlled hybridisation with mass selection. This research project was carried out to assist the Australian almond improvement program in the areas of micropropagation, cryopreservation, and rapid identification of self-incompatibility genotypes of almond. Micropropagation was accomplished successfully for two commercially important almond cultivars ('Nonpareil' 15-1 and 'Ne Plus Ultra') and an almond/peach hybrid rootstock by culturing shoot tips, about 0.7 cm long with 3 - 5 leaves, on appropriate shoot multiplication media. For 'Nonpareil' 15-1, AP medium with 0.049 uM IBA, 3 uM BAP, 0.058 M sucrose, and 0.7% agar at pH 5.7 was effective. MS medium with 0.049 uM IBA, 5 uM BAP, 0.088 M sucrose, and 0.7% agar at pH 5.7 was suitable for 'Ne Plus Ultra'. For the almond/peach hybrid rootstock, MS medium supplemented with 10 uM BAP, 0.088 M sucrose, and 0.7% agar provided the best shoot proliferation. Shoots of the rootstock, about two cm long, readily produced roots after one week in the dark and two weeks in the light on half strength MS medium supplemented with 2.4 uM IBA...

'Florida Radiance' Strawberry

Chandler, Craig K. ( Author, Primary ); Santos, Bielinski M ( Author, Secondary ); Peres, Natalia A. ( Author, Secondary ); Jouquand, Celine ( Author, Secondary ); Plotto, Anne ( Author, Secondary ); Sims, Charles A. ( Author, Secondary )
Fonte: American Society of Horticultural Science; American Society of Horticultural Science Publicador: American Society of Horticultural Science; American Society of Horticultural Science
Tipo: mixed material Formato: Journal Article
ENGLISH
Relevância na Pesquisa
46.03%
(Acquisition) Collected for University of Florida's Institutional Repository by the UFIR Self-Submittal tool. Submitted by Suzanne Stapleton.; (Publication Status) Published; (Preferred Citation) HORTSCIENCE 44(6):1769–1770. 2009; (Additional Physical Form) NALT Terms: Fragaria ananassa, strawberries, plant breeding, germplasm releases, cultivar identification, agronomic traits, pedigree, Florida

Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars

Rojas, Gabriela; Muñoz, Carlos; Hinrichsen, Patricio; Lemus, Gamalier; Méndez, Marco A.
Fonte: UNIV CATOLICA DE VALPARAISO Publicador: UNIV CATOLICA DE VALPARAISO
Tipo: Artículo de revista
EN
Relevância na Pesquisa
45.92%
The genetic characterization of 117 peach and nectarine cultivars (Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. Analyzed genotypes include the complete list of cultivars under intellectual property (IP) protection in Chile. One hundred and two out of the 117 cultivars under study could be identified using only 7 SSRs. Other 5 cultivars were differentiated using 3 additional markers, but 5 pairs of genotypes were not differentiable. The average expected heterozygosity for the set of markers was 0.55, ranging from 0.28 in BPPCT-008 to 0.81 in CPPCT-022, with an F value of 0.37. A Neighbor-Joining dendrogram showed that, with few exceptions, peaches and nectarines clustered separately. These results are the basis for the development of a fingerprinting protocol for the unequivocal identification of most of the peach and nectarine cultivars officially registered in Chile.; Financed by FONDEF-Chile, Grant D04I-1060.

The Portuguese grapevine cultivar 'Amaral': synonymies, homonymies

Castro, Isaura; Martín, Juan Pedro; Ortiz, Jesús María; Mota, Maria Teresa; Pinto-Carnide, Olinda
Fonte: Universidade de Trás-os-Montes e Alto Douro Publicador: Universidade de Trás-os-Montes e Alto Douro
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
46.34%
Within Western Europe, Portugal is the country with highest diversity of grapevine cultivars. As the Iberian Peninsula has been considered a domestication centre of grapevines, the interest on Portuguese grapevine cultivars diversity has increased in the last years. Using twelve SSR markers scattered across twelve grape chromosomes, thirty-three accessions collected on Vinhos Verdes and Lafões Controlled Designations of Origin (DOC) and supposedly of the grapevine cultivar 'Amaral', were analysed. Names of the accessions ranged from 'Amaral' and 'Azal Tinto' to 'Sousão Galego'. Eight different genotypes were detected. The majority of accessions had the genotype attributed to 'Amaral'. However, three accessions were identified as misnaming cases and four genotypes were considered unknown, as no cultivar identification was achieved. Nevertheless, not only the misnamed cultivars, 'Vinhão', 'Melhorio' and 'Touriga Nacional', but also the four unknown genotypes, 'Azal Tinto' (AT2203 and AT2206), 'Amaral-1', 'Amaral-2' and 'Sousão Galego', share at least one allele in each of the 12 SSR loci analysed. The accessions AT2203 and AT2206 were found as possible offspring of 'Amaral' and 'Folgasão' and the accession 'Amaral-2' is likely result of a cross between 'Amaral' and 'Pedral'. The high number of synonym names that are attributed to 'Amaral'...

Varietal differences among the flavonoid profiles of white grape cultivars studied by high-performance liquid chromatography

Masa Vázquez, Antón; Vilanova de la Torre, María del Mar; Pomar, F.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 272896 bytes; application/msword
ENG
Relevância na Pesquisa
35.92%
In order to develop a method that allows to distinguish between white grape cultivars, the flavonoid profiles of 10 white accessions from the “Misión Biológica de Galicia” germplasm collection were studied during years 2003, 2004 and 2005 by high-performance liquid chromatography (HPLC). Twenty-four flavonoids (15 flavonols and 9 dihydroflavonols) were totally or partly identified, and significant differences between the studied flavonoid markers were found. With this method all the cultivars examined could be easily distinguished from each other, and we may conclude that this has been proved to be of great value for white grape cultivar recognition.; This study was financially supported by the project XUGA40301B94 from the Galician government (Spain). M.V. was supported by a I3P contract financed by the CSIC-European Social Fund.; Peer reviewed

Varietal differences among the anthocyanin profiles of 50 red table grape cultivars studied by high performance liquid chromatography

Pomar, F.; Novo, M.; Masa Vázquez, Antón
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 37888 bytes; application/msword
ENG
Relevância na Pesquisa
36.01%
In order to develop a method that allows to distinguish between grape cultivars, the anthocyanin profiles of 50 accessions from the “Misión Biológica de Galicia” germplasm collection were studied by high performance liquid chromatography (HPLC). Nineteen anthocyanins were totally or partly identified and significant quantitative differences between the studied anthocyanin markers were found. With this method all 50 cultivars examinated could be easily distinguished from each other. In addition, the HPLC fingerprints and the relative-area anthocyanins plot for every cultivar has been elaborated and stored in a database. To test the validity of this method, several unknown samples have been analysed comparing their anthocyanin profile with the fingerprint database, and we may conclude that this has been proved to be of great value for grape cultivar recognition.; This study was financially supported by the project XUGA-40301B97 from the Galician government in Spain and partially by the project RF99-110 (INIA) from the Spanish government.; Peer reviewed

Identification of phenolic compounds in tissues of the novel olive cultivar Hardys Mammoth

Ryan, Danielle; Antolovich, Michael; Herlt, Anthony; Prenzler, Paul D; Lavelle, Janet; Robards, Kevin
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
46.18%
A methodological approach to phenolic profiling making extensive use of LC-MS with extracted ion chromatograms was applied to extracts of five different olive tissues: pulp, seed, stone, new-season leaves, and old-season leaves. Tissue extracts of the cultivars Hardy's Mammoth, Corregiola, Verdale, and Manzanillo were analyzed by HPLC with UV and ESI MS detection. Chromatograms of samples of green Hardy's Mammoth drupes, a uniquely Australian olive cultivar, were dominated by a large, broad peak. This peak was not attributable to oleuropein, which is usually the dominant phenolic compound in green olive fruit, but the phenolic compound I. This compound was isolated by semipreparative HPLC and characterized by 1D- and 2D-NMR. Extraction studies showed that the compound was not likely to be an artifact of an enzymatic degradation process. Tritium labeling studies were used to establish a possible relationship between the biosynthesis of I and oleuropein.

Caracterização da diversidade genética de linhagens comerciais de Coffea arabica através de marcadores moleculares do tipo RAPD, AFLP e SSR; Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems

Maluf, Mirian Perez; Silvestrini, Milene; Ruggiero, Luciana Machado de Campos; Guerreiro Filho, Oliveiro; Colombo, Carlos Augusto
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/08/2005 ENG
Relevância na Pesquisa
46.24%
One of the greatest problems in Coffea arabica breeding is identifying precisely any inbred line, based only on botanical and agronomical descriptors, because of the reduced genetic variability of the species, close pedigree origin, which results in small phenotypic variation. Recently, molecular markers have been used for plant germplasm characterization and identification in several commercial species. This work evaluates the reliability of three marker systems: RAPD, AFLP and SSR, to characterize the genetic variability of commercially-used Coffea inbred lines developed by the Instituto Agronômico (IAC), and their potential for cultivar identification. All methods identified polymorphisms among the cultivars. The genetic diversity recognized by the methods is very similar, although is very narrow. RAPD and SSR marker systems grouped more efficiently the evaluated cultivars according to parental origin. None of the methods allowed inbred line identification. Therefore for varietal protection, it would be necessary using a combination of botanical, agronomical and molecular markers descriptors for precise cultivar identification.; A identificação de linhagens de Coffea arabica a partir de descritores botânicos e agronômicos é um problema para o desenvolvimento de cultivares. Basicamente...

Molecular markers to study the variability within the Eragrostis curvula complex

Zappacosta,D; Meier,M; Carrera,A; Pacheco,G; Cardone,S; Selva,JP; Echenique,V
Fonte: Phyton (Buenos Aires) Publicador: Phyton (Buenos Aires)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 EN
Relevância na Pesquisa
46.03%
Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in order to assess the reproductive mode through progeny tests, to determine intracultivar homogeneity or seed purity, to establish genetic relationships among the cultivars within the complex and to characterize the new materials obtained by our group. Eight commercial cultivars and three new plant materials were analyzed. Uniform and variable patterns were observed in progenies of apomictic and sexual plants, respectively. Seed purity was evaluated in seed bulks, observing a certain degree of contamination with seeds from different sources. AFLP were the markers with the highest potential for cultivar identification. The clustering using SSR and AFLP was consistent with previous studies using isozymes and morphological traits. The new tetraploid materials developed by our own group should be included within the curvula type. We also proposed the creation of a new morphological type for the diploid...