Página 1 dos resultados de 1477 itens digitais encontrados em 0.005 segundos

Evaluating a bioremediation tool for atrazine contaminated soils in open soil microcosms: The effectiveness of bioaugmentation and biostimulation approaches

Lima, D.; Viana, P.; André, S.; Chelinho, S.; Costa, C.; Ribeiro, R.; Sousa, J. P.; Fialho, A. M.; Viegas, C. A.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.08%
A previously developed potential cleanup tool for atrazine contaminated soils was evaluated in larger open soil microcosms for optimization under more realistic conditions, using a natural crop soil spiked with an atrazine commercial formulation (Atrazerba FL). The doses used were 20£ or 200£ higher than the recommended dose (RD) for an agricultural application, mimicking over-use or spill situations. Pseudomonas sp. strain ADP was used for bioaugmentation (around 107 or 108 viable cells g¡1 of soil) and citrate for biostimulation (up to 4.8 mg g¡1 of soil). Bioremediation treatments providing fastest and higher atrazine biodegradation proved to differ according to the initial level of soil ontamination. For 20£ RD of Atrazerba FL, a unique inoculation with Pseudomonas sp. ADP (9 ± 1 £ 107 CFU g¡1) resulted in rapid atrazine removal (99% of the initial 7.2 ± 1.6 lg g¡1 after 8 d), independent of citrate. For 200£ RD, an inoculation with the atrazine- degrading bacteria (8.5 ± 0.5 £ 107 CFU g¡1) supplemented with citrate amendment (2.4 mg g¡1) resulted in improved biodegradation (87%) compared with bioaugmentation alone (79%), even though 7.8 ± 2.1 lg of atrazine g¡1 still remained in the soil after 1 wk. owever, the same amount of inoculum...

EX-SITU BIOREMEDIATION OF BRAZILIAN SOIL CONTAMINATED WITH PLASTICIZERS PROCESS WASTES

Ferreira, I. D.; Morita, D. M.
Fonte: BRAZILIAN SOC CHEMICAL ENG; SAO PAULO Publicador: BRAZILIAN SOC CHEMICAL ENG; SAO PAULO
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.18%
The aim of this research was to evaluate the bioremediation of a soil contaminated with wastes from a plasticizers industry, located in Sao Paulo, Brazil. A 100-kg soil sample containing alcohols, adipates and phthalates was treated in an aerobic slurry-phase reactor using indigenous and acclimated microorganisms from the sludge of a wastewater treatment plant of the plasticizers industry (11gVSS kg(-1) dry soil), during 120 days. The soil pH and temperature were not corrected during bioremediation; soil humidity was corrected weekly to maintain 40%. The biodegradation of the pollutants followed first-order kinetics; the removal efficiencies were above 61% and, among the analyzed plasticizers, adipate was removed to below the detection limit. Biological molecular analysis during bioremediation revealed a significant change in the dominant populations initially present in the reactor.; FAPESP (The State of Sao Paulo Research Foundation) [06/50487- 0]; FAPESP (The State of Sao Paulo Research Foundation)

Bioremediation of Herbicide Velpar K (R) In Vitro in Aqueous Solution with Application of EM-4 (Effective Microorganisms)

Ramos, Marcio Antonio Gomes; Yoshioka, Sergio Akinobu
Fonte: INST TECNOLOGIA PARANA; CURITIBA-PARANA Publicador: INST TECNOLOGIA PARANA; CURITIBA-PARANA
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.25%
This work assessed the bioremediation of herbicide Velpar K (R), in vitro in aqueous solution, used against weeds in sugar cane in Sao Paulo state. The herbicide contained Hexazinone and Diuron. It was used the microbial inoculant denominated Effective Microorganisms (EM-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. Results for the depth of cultivation on agar-agar inoculated with EM-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the Velpar K (R) in the gel. The analysis of high performance liquid chromatography ( HPLC) showed that the EM-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron and 70% for hexazinone after 21 days in solution of 2:1 of Velpar K (R)/EM-4 ratio. These results could be useful for planning the bioremediation of contaminated areas with Velpar K (R).

Ex-situ bioremediation of Brazilian soil contaminated with plasticizers process wastes

Ferreira, I. D.; Morita, D. M.
Fonte: Brazilian Society of Chemical Engineering Publicador: Brazilian Society of Chemical Engineering
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.18%
The aim of this research was to evaluate the bioremediation of a soil contaminated with wastes from a plasticizers industry, located in São Paulo, Brazil. A 100-kg soil sample containing alcohols, adipates and phthalates was treated in an aerobic slurry-phase reactor using indigenous and acclimated microorganisms from the sludge of a wastewater treatment plant of the plasticizers industry (11gVSS kg-1 dry soil), during 120 days. The soil pH and temperature were not corrected during bioremediation; soil humidity was corrected weekly to maintain 40%. The biodegradation of the pollutants followed first-order kinetics; the removal efficiencies were above 61% and, among the analyzed plasticizers, adipate was removed to below the detection limit. Biological molecular analysis during bioremediation revealed a significant change in the dominant populations initially present in the reactor.

Bioremediation of herbicide velpar K® in vitro in aqueous solution with application of EM-4 (effective microorganisms)

Ramos, Márcio Antônio Gomes; Yoshioka, Sergio Akinobu
Fonte: Brazilian Archives of Biology and Technology Publicador: Brazilian Archives of Biology and Technology
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.25%
This work assessed the bioremediation of herbicide Velpar K®, in vitro in aqueous solution, used against weeds in sugar cane in São Paulo state. The herbicide contained Hexazinone and Diuron. It was used the microbial inoculant denominated Effective Microorganisms (EM-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. Results for the depth of cultivation on agar-agar inoculated with EM-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the Velpar K®in the gel. The analysis of high performance liquid chromatography (HPLC) showed that the EM-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron and 70% for hexazinone after 21 days in solution of 2:1 of Velpar K®/EM-4 ratio. These results could be useful for planning the bioremediation of contaminated areas with Velpar K®.

The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil

Mariano, Adriano Pinto; Crivelaro, Sérgio Henrique Rezende; Angelis, Dejanira de Franceschi de; Bonotto, Daniel Marcos
Fonte: Brazilian Archives of Biology and Technology Publicador: Brazilian Archives of Biology and Technology
Tipo: Artigo de Revista Científica Formato: 1043-1055
ENG
Relevância na Pesquisa
37.25%
Este trabalho investigou a possibilidade de se usar a vinhaça como um agente estimulador de processos de biorremediação ex-situ. Amostras de água subterrânea e solo foram coletadas em três postos de combustíveis. A biorremediação do solo foi simulada em frascos de Bartha, usados para medir a produção de CO2, durante 48 dias, onde a vinhaça foi adicionada a uma concentração de 33 mL.Kg-1 de solo. A eficiência de biodegradação também foi medida pela quantificação de hidrocarbonetos totais de petróleo (TPH) por cromatografia gasosa. A biorremediação da água subterrânea foi realizada em experimentos laboratoriais simulando condições aeradas (bioreatores) e não aeradas (frascos de DBO). em ambos os casos, a concentração de vinhaça foi de 5 % (v/v) e diferentes parâmetros físico-químicos foram avaliados durante 20 dias. Embora um aumento da fertilização e da população microbiana do solo foram obtidos com a vinhaça, esta estratégia não se mostrou adequada em aumentar a eficiência da biorremediação dos solos contaminados com óleo diesel. A adição de vinhaça às águas subterrâneas contaminadas teve efeitos negativos na biodegradação dos hidrocarbonetos, uma vez que a vinhaça, como uma fonte de carbono facilmente assimilável...

Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station

Mariano, Adriano Pinto; Kataoka, Ana Paula de Arruda Geraldes; Angelis, Dejanira de Franceschi de; Bonotto, Daniel Marcos
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: 346-353
ENG
Relevância na Pesquisa
37.18%
O objetivo do presente estudo foi investigar possíveis métodos para aumentar a taxa de biodegradação aeróbia de hidrocarbonetos (tratamentos ex-situ). Neste trabalho, processos de biorremediação foram aplicados a um solo arenoso com alto nível de contaminação ocasionada por um vazamento de um tanque de armazenamento de óleo diesel subterrâneo em um posto de combustíveis. Experimentos em escala laboratorial (respirômetros de Bartha) foram utilizados para avaliar a biodegradação do óleo diesel. Estímulo da biodegradação foi realizado utilizando-se as técnicas de bioestímulo (adição de soluções de nitrogênio e fósforo ou surfactante Tween 80) e de bioaumento (consórcio bacteriano isolado de um sistema de landfarming). Para investigar as interações entre os fatores otimizadores, e encontrar a melhor combinação entre esses agentes, o estudo foi baseado em um delineamento experimental fatorial completo. A eficiência de biodegradação foi simultaneamente medida com dois métodos: respirométrico (produção de CO2 microbiano) e cromatografia gasosa. Testes de toxicidade aguda com Daphnia similis foram aplicados para examinar a eficiência dos processos em termos de geração de produtos menos tóxicos. Resultados mostraram que todas as estratégias de biorremediação aceleraram a biorremediação natural do solo contaminado e os melhores resultados foram obtidos quando os tratamentos tinham adição de nutrientes. Dados respirométricos indicaram uma máxima mineralização de hidrocarbonetos de 19...

Degradação de compostos aromaticos por microormanismos haloficos e aplicação destes na biorremediação da agua de petroleo; Degradation of aromatic compounds microoganism and their application in bioremediation of water production oil

Maricy Raquel Lindenbah Bonfa
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 28/01/2009 PT
Relevância na Pesquisa
37.08%
Os microrganismos halofilicos sao aqueles capazes de viver em ambientes salinos. Dentre eles encontram-se, por exemplo, as bacterias halofilicas e as haloarqueias. Estas duas classes de organismos foram o objeto de estudo deste projeto. Estes microrganismos foram testados quanto a habilidade de degradacao de compostos monoaromaticos e poliaromaticos. A capacidade de degradacao destes compostos por microrganismos halofilicos e de extrema importancia ja que ambientes salinos estao sujeitos a contaminacoes por compostos toxicos, alem de muitos efluentes industriais, como por exemplo, a agua de producao de petroleo, possuir alta salinidade. As linhagens de haloarqueias testadas apresentaram a capacidade de crescer e degradar acidos aromaticos como unica fonte de carbono e energia. A degradacao foi menor em presenca de 0,05% de extrato de levedura. Estas mesmas linhagens tambem apresentaram a capacidade de degradar HAP em presenca de 0,05% de extrato de levedura. Foram utilizados primers para a amplificacao de genes que sintetizam catecol 1,2-dioxigenase, protocatecuato 3,4-dioxigenase e gentisato 1,2-dioxigenase. Somente a linhagem Halococcus morhuaea apresentou o gene da gentisato 1,2-dioxigenase. As haloarqueias estudadas foram identificadas atraves da amplificacao e sequenciamento do gene RNAr 16S e foram identificadas como pertencentes ao genero Haloferax. Foram isoladas 7 linhagens de haloarqueias a partir de coletas realizadas no Brasil (Nossa Senhora do Socorro-SE e Arraial do Cabo-RJ)...

Avaliação do potencial de microbiota originada de reservatórios de petróleo para biorremediação = : Evaluation of bioremediation potential of microorganisms from petroleum reservoirs; Evaluation of bioremediation potential of microorganisms from petroleum reservoirs

Bruna Martins Dellagnezze
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 22/01/2015 PT
Relevância na Pesquisa
37.25%
A poluição é um problema mundial amplamente discutido, incluindo os derramamentos de petróleo ocorridos através de acidentes ou por atividades humana, os quais acarretam grande impacto ambiental e econômico. O processo de biorremediação utiliza micro-organismos, associados ou não a outros compostos como biossurfactantes e até mesmo enzimas, com o objetivo de transformar compostos orgânicos em inorgânicos, levando à formação de compostos inertes ou não tóxicos. Deste modo, a biorremediação representa um modo efetivo e sustentável para se tratar áreas contaminadas. Neste trabalho foi possível avaliar o potencial de clones metagenômicos obtidos a partir da construção de uma biblioteca fosmidial e de linhagens de bactérias, todos provenientes de amostras de petróleo de reservatórios brasileiros em escala de microcosmos e mesocosmos, visando futura aplicação em processos de biorremediação. Em um primeiro ensaio os micro-organismos foram avaliados na forma livre, em 50 mL de água do mar artificial e petróleo bruto como única fonte de carbono, a cada sete dias durante 21 dias. Posteriormente, os micro-organismos com melhor potencial de biodegradação foram selecionados e aprisionados em esferas de quitosana e testados novamente em microcosmos...

Partial characterization of biosurfactant from Lactobacillus pentosus and comparison with sodium dodecyl sulphate for the bioremediation of hydrocarbon contaminated soil

Moldes, Ana; Paradelo, R.; Vecino, X.; Cruz, J. M.; Gudiña, Eduardo J.; Rodrigues, L. R.; Teixeira, J. A.; Dominguez, José Manuel; Barral, M. T.
Fonte: Hindawi Publishing Corporation; Hindawi Publishing Corp. Publicador: Hindawi Publishing Corporation; Hindawi Publishing Corp.
Tipo: Artigo de Revista Científica
Publicado em //2013 ENG
Relevância na Pesquisa
37.18%
The capability of a cell bound biosurfactant produced by Lactobacillus pentosus, to accelerate the bioremediation of a hydrocarbon-contaminated soil, was compared with a synthetic anionic surfactant (sodium dodecyl sulphate SDS-). The biosurfactant produced by the bacteria was analyzed by Fourier transform infrared spectroscopy (FTIR) that clearly indicates the presence of OH and NH groups, C=O stretching of carbonyl groups and NH nebding (peptide linkage), as well as CH2–CH3 and C–O stretching, with similar FTIR spectra than other biosurfactants obtained from lactic acid bacteria. After the characterization of biosurfactant by FTIR, soil contaminated with 7,000 mg Kg−1 of octane was treated with biosurfactant from L. pentosus or SDS. Treatment of soil for 15 days with the biosurfactant produced by L. pentosus led to a 65.1% reduction in the hydrocarbon concentration, whereas SDS reduced the octane concentration to 37.2% compared with a 2.2% reduction in the soil contaminated with octane in absence of biosurfactant used as control. Besides, after 30 days of incubation soil with SDS or biosurfactant gave percentages of bioremediation around 90% in both cases. Thus, it can be concluded that biosurfactant produced by L. pentosus accelerates the bioremediation of octane-contaminated soil by improving the solubilisation of octane in the water phase of soil...

Ex-situ bioremediation of Brazilian soil contaminated with plasticizers process wastes

Ferreira,I. D.; Morita,D. M.
Fonte: Brazilian Society of Chemical Engineering Publicador: Brazilian Society of Chemical Engineering
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2012 EN
Relevância na Pesquisa
37.18%
The aim of this research was to evaluate the bioremediation of a soil contaminated with wastes from a plasticizers industry, located in São Paulo, Brazil. A 100-kg soil sample containing alcohols, adipates and phthalates was treated in an aerobic slurry-phase reactor using indigenous and acclimated microorganisms from the sludge of a wastewater treatment plant of the plasticizers industry (11gVSS kg-1 dry soil), during 120 days. The soil pH and temperature were not corrected during bioremediation; soil humidity was corrected weekly to maintain 40%. The biodegradation of the pollutants followed first-order kinetics; the removal efficiencies were above 61% and, among the analyzed plasticizers, adipate was removed to below the detection limit. Biological molecular analysis during bioremediation revealed a significant change in the dominant populations initially present in the reactor.

The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil

Mariano,Adriano Pinto; Crivelaro,Sérgio Henrique Rezende; Angelis,Dejanira de Franceschi de; Bonotto,Daniel Marcos
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2009 EN
Relevância na Pesquisa
37.25%
This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days. Although an increase in the soil fertility and microbial population were obtained with the vinasse, it demonstrated not to be adequate to enhance the bioremediation efficiency of diesel oil contaminated soils. The addition of the vinasse in the contaminated groundwaters had negative effects on the biodegradation of the hydrocarbons, since vinasse, as a labile carbon source, was preferentially consumed.

Bioremediation of herbicide velpar K® in vitro in aqueous solution with application of EM-4 (effective microorganisms)

Ramos,Márcio Antônio Gomes; Yoshioka,Sergio Akinobu
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2012 EN
Relevância na Pesquisa
37.25%
This work assessed the bioremediation of herbicide Velpar K®, in vitro in aqueous solution, used against weeds in sugar cane in São Paulo state. The herbicide contained Hexazinone and Diuron. It was used the microbial inoculant denominated Effective Microorganisms (EM-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. Results for the depth of cultivation on agar-agar inoculated with EM-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the Velpar K®in the gel. The analysis of high performance liquid chromatography (HPLC) showed that the EM-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron and 70% for hexazinone after 21 days in solution of 2:1 of Velpar K®/EM-4 ratio. These results could be useful for planning the bioremediation of contaminated areas with Velpar K®.

Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station

Mariano,Adriano Pinto; Kataoka,Ana Paula de Arruda Geraldes; Angelis,Dejanira de Franceschi de; Bonotto,Daniel Marcos
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2007 EN
Relevância na Pesquisa
37.18%
The purpose of the present study was to investigate possible methods to enhance the rate of aerobic biodegradation of hydrocarbons (ex-situ treatments). In this work, the bioremediation processes were applied to a sandy soil with a high level of contamination originated from the leakage of a diesel oil underground storage tank at a petrol station. Laboratory scale experiments (Bartha biometer flasks) were used to evaluate the biodegradation of the diesel oil. Enhancement of biodegradation was carried out through biostimulation (addition of nitrogen and phosphorus solutions or Tween 80 surfactant) and bioaugmentation (bacterial consortium isolated from a landfarming system). To investigate interactions between optimizing factors, and to find the right combination of these agents, the study was based on full factorial experimental design. Efficiency of biodegradation was simultaneously measured by two methods: respirometric (microbial CO2 production) and gas chromatography. Acute toxicity tests with Daphnia similis were applied for examination of the efficiency of the processes in terms of the generation of less toxic products. Results showed that all bioremediation strategies enhanced the natural bioremediation of the contaminated soil and the best results were obtained when treatments had nutritional amendment. Respirometric data indicated a maximum hydrocarbon mineralization of 19.8%...

Metal Sulfide Formation by the Cyanobacterium Synechococcus leopoldiensis as a Mechanism of Toxic Metal Bioremediation

Raykin, Leon
Fonte: Quens University Publicador: Quens University
Tipo: Tese de Doutorado Formato: 943104 bytes; application/msword
EN
Relevância na Pesquisa
37.08%
The contamination of water bodies with toxic metals is an environmental issue that can be addressed through several approaches. One approach is bioremediation, in which living organisms are used to detoxify the contaminant. Recent studies have shown that a number of cyanobacteria are able to detoxify mercury by forming insoluble mercury sulfide precipitates. In this study, one of these organisms, Synechococcus leopoldiensis, was studied to determine whether it also forms metal sulfides with lead, arsenic or cadmium. Lead readily precipitated in the cyanobacteria culture medium. This interfered with biomass measurements and decreased the bioavailability of the metal, complicating the study of lead detoxification by S. leopoldiensis. Application of additional sulfate to the culture medium increased the toxicity of lead to the cyanobacterium, suggesting that a decrease in pH associated with sulfate application was increasing the solubility of the lead precipitates. Growth was initially inhibited in S. leopoldiensis cultures incubated with 15 and 30 ppm arsenic, but the cyanobacteria eventually recovered to a biomass not statistically different from that of control cultures incubated in parallel. The recovery was enhanced in 15 ppm cultures that had additional sulfate added to the culture medium...

Metal-sulfide based resistance to cadmium toxicity in the cyanobacterium, Synechococcus leopoliensis and the potential utilization for bioremediation.

Direnfeld, Samuel Mark
Fonte: Quens University Publicador: Quens University
Tipo: Artigo de Revista Científica Formato: 261023 bytes; application/pdf
EN
Relevância na Pesquisa
37.25%
Environmental metal contamination is prevalent and has serious consequences for human health and ecosystem viability. A number of methods have been applied to environmental metal detoxification, including bioremediation. Bioremediation systems use biological materials produced by organisms to detoxify environmental contaminants. Previous studies have shown that Synechococcus leopoliensis (Raciborski), a cyanobacterium, is an excellent candidate for utilization in metal bioremediation systems. These studies also demonstrated that metal-sulfide formation is likely the primary form of metal detoxification in cyanobacteria. Metal-sulfides are insoluble and are therefore, not biologically available to cause damage. In this study the cadmium remediation potential of S. leopoliensis was analyzed. Sulfate and cysteine supplementation enhanced the ability of the cyanobacteria to remediate cadmium [Cd(II)]. This is likely because a greater availability of sulfur nutrient resources enhanced the potential for cadmium-sulfide formation. There was significantly more growth in the 10x sulfate supplemented cyanobacteria than the control inoculated with 0.47 and 0.62 ppm Cd(II). At concentrations of 0.31 ppm Cd(II) and below, there was no difference in growth between the sulfate supplemented and control cyanobacteria...

Establishment of a bioremediation facility in South Australia - research and commercial potential

McClure, Nicholas Charles; Dandi, Cathy; Bentham, Richard H.; Franco, Chris; Singleton, Ian
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Artigo de Revista Científica
Publicado em //1997 EN
Relevância na Pesquisa
37.25%
Centralised bioremediation facilities are relatively common in the U.S.A., Canada and some parts of Europe. Whilst there is a preference throughout most States in Australia for conducting soil remediations on-site, in some cases this is either technically difficult or hinders rapid development which would recoup remediation costs. Remediation of contaminated soil at a remote facility has a number of additional advantages; it allows increased timescales for achieving minimum residual pollutant levels, reduces individual site preparation costs and increases the potential for conducting longer term research projects. This paper discusses the establishment of a centralised commercial and research bioremediation facility in South Australia, describes ongoing bioremediation projects being undertaken and identifies research needs relevant to ex situ soil bioremediation in the Australasian region.; Nick C. McClure, Cathy Dandie, Richard Bentham, Chris Franco and Ian Singleton

Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

Fuentes, María Soledad; Alvarez, Analia; Sáez, Juliana María; Benimeli, Claudia Susana; Amoroso, Maria Julia del R.
Fonte: Springer Publicador: Springer
Tipo: info:eu-repo/semantics/article; info:ar-repo/semantics/artículo; info:eu-repo/semantics/publishedVersion Formato: application/pdf
ENG
Relevância na Pesquisa
37.08%
Methoxychlor is an organochlorine pesticide used worldwide against several insect pests, resulting in human exposure. This pesticide mimics endocrine hormone functions, interfering with normal endocrine activity in humans and wildlife. For this reason, it is imperative to develop methods to remove this pesticide from the environment, and though, bioremediation using microorganisms results as an excellent strategy. Five Streptomyces spp. strains previously isolated from organochlorine-polluted sites and capable to grow and remove methoxychlor were combined as different mixed cultures to increase methoxychlor removal. From the 39 consortia tested, one consortium (Streptomyces spp. A6, A12, A14, M7) was selected because of its high pesticide removal and specific dechlorinase activity to be assayed on slurry and soil systems. This consortium showed higher biomass values (8.3 9 106 ± 5.7 9 105 CFU mL-1) and methoxychlor removal (56.2 ± 2.3 %) on enriched slurry than in nonenriched slurry (7.3 9 105 ± 1.2 9 105 CFU mL-1 and 45.6 ± 7.4 % of pesticide removal). In soil systems, Streptomyces consortium showed higher growth (1.0 9 1011 ± 5.0 9 1010 CFU g-1) than in enriched slurry, although differences in methoxychlor removal between both culture conditions were not statistically significant. Therefore...

Bioremediation of oil refinery sludge by landfarming in semiarid conditions: Influence on soil microbial activity

Marín, Antonio; Hernández Fernández Muñoz, María Teresa; García Izquierdo, Carlos
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
37.08%
11 pages, 5 figures, 4 tables.; Bioremediation of a refinery sludge containing hydrocarbons in a semi-arid climate using landfarming techniques is described. The objective of this study was to assess the ability of this technique to reduce the total hydrocarbon content added to the soil with the refinery sludge in semiarid climate (low rain and high temperature). In addition, we have evaluted the effect of this techique on the microbial activity of the soil involved. For this, biological parameters (carbon fractions, microbial bilmass carbon, basal respiration and ATP) and biochemical parameters(different enzymatic activities) were determined. The results showed that 80% of the hydrocarbons were eiminated in eleven months, half of this reduction taking place during the first three months. The labile carbon fractions, MBC, basal respiration and ATP of the soils submitted to landfarming showed higher values than the control soil during the first months of the process, although these values fell down by the end of the experimental period as the hydrocarbons were degraded by mineralisation. All the enzymatic activities studied: oxydoreductases such as dehydrogenase activity, and hydrolases of C(β-glucosidase activity) and N Cycle (urease and protease) showed higher values in the soils amended with the refinery sludge than in the control. As in the case of the previous parameters...

Assessment of in situ bioremediation of oil contaminated soil and groundwater in a petroleum refinery: a laboratory soil column study

Zargar, M.; Sarrafzadeh, M.H.; Taheri, B.; Keshavarz, A.
Fonte: Taylor & Francis Publicador: Taylor & Francis
Tipo: Artigo de Revista Científica
Publicado em //2014 EN
Relevância na Pesquisa
37.18%
Precipitation and seasonal water level fluctuations mostly add to the problem of soil and groundwater contamination and lead to pollution of capillary fringe layer and exacerbation of groundwater contamination. At the Tehran Oil Refining Company (TORC), with critical problem of soil and groundwater pollution, finding a suitable remediation method has been a big concern. As bioremediation is one of the most economically and technically attractive decontamination methods, it was chosen for preliminary study to solve this problem. For simulation of the region, soil column treatment method by using oil habituated indigenous microorganisms of the area was selected. The main purpose was assessing the suitability of bioremediation method in the target area for treatment of capillary fringe layer. Two columns (one as treatment column and the other as a control) were designed and the variation of different parameters including dissolved oxygen (DO), pH, the number of viable and active microorganisms (CFU/mL), and biodegradation rate during 30 working days were recorded. The experiments showed that a major part of degrading microorganisms were facultative anaerobic. pH was not an inhibiting factor and the number of active microorganisms was increasing during aeration time. The results of solvent extraction method also revealed that during 30 days...