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Chemical composition and biological properties of portuguese wild mushrooms: a comprehensive study

Barros, Lillian; Venturini, Bruna A.; Baptista, Paula; Estevinho, Leticia M.; Ferreira, Isabel C.F.R.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
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56.53%
The chemical composition and biological properties of Portuguese wild mushrooms (Cantharellus cibarius, Hypholoma fasciculare, Lepista nuda, Lycoperdon molle, Lycoperdon perlatum, Ramaria botrytis, Tricholoma acerbum) were evaluated in order to assess these products as sources of nutrients and nutraceuticals. The analyzed mushrooms contain very useful phytochemicals such as phenolics, tocopherols, ascorbic acid, and carotenoids. All of the species proved to have antioxidant activity (measured by four different methods), being more significant for R. botrytis (EC50 values < 1 mg/ mL). Lycoperdon species were resistant to all of the tested microorganisms, and the other samples revealed antimicrobial activity selectively against Gram-positive bacteria, with very low minimal inhibitory concentration, in some cases, even lower than the standard. The combination of bioactive compounds and rich nutritional composition (high contents in protein and carbohydrates, low content in fat with the precious contribution of unsaturated fatty acids and the absence of trans fatty acids) in the mushroom makes it a very special food

Validation of an electrothermal atomization atomic absorption spectrometry method for quantification of total Chromium and Chromium(VI) in wild mushrooms and underlying soils

Figueiredo, Ana E.; Soares, Maria Elisa; Baptista, Paula; Castro, Marisa; Bastos, Maria de Lourdes
Fonte: ACS Publications Publicador: ACS Publications
Tipo: Artigo de Revista Científica
ENG
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56.53%
An ETAAS method was validated to quantify total Cr and CrVI in mushrooms and the underlying soils. The method includes a sample pretreatment for total Cr dissolution using a wet acid digestion procedure and a selective alkaline extraction for CrVI. The limits of detection were, expressed in íg/L, 0.15 and 0.17 for total Cr and CrVI, respectively. The linearity ranges under the optimized conditions were 0.15-25.0 and 0.17-20.0 íg/L for total Cr and CrVI, respectively. The limits of quantification were, expressed in íg/g of dry weight, 0.0163 and 0.0085 for total and hexavalent chromium, respectively. The precision of the instrumental method for total Cr and CrVI was lower than 1.6%, and for the analytical method, it was lower than 10%. The accuracy of the method for CrVI quantification was evaluated by the standard additions method, with the recoveries being higher than 90% for all of the added concentrations. For total Cr, certified reference materials (lichen CRM 482 and soil sample NCS ZC73001) were used. An interference study was also carried out in a mushroom simulated matrix, and it was verified that the deviations of the expected values were lower than 4.0% for both total Cr and CrVI. The validated method was applied to the evaluation of total Cr and CrVI in 34 wild mushrooms and 34 respective underlying soil samples collected in two different regions of Portugal (Beira Interior and Trás-os-Montes)...

A QCAR model for predicting antioxidant activity of wild mushrooms

Froufe, Hugo J.C.; Abreu, Rui M.V.; Ferreira, Isabel C.F.R.
Fonte: Taylor & Francis Publicador: Taylor & Francis
Tipo: Artigo de Revista Científica
ENG
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66.65%
Wild mushrooms have been described as sources of natural antioxidants, particularly phenolic compounds. However, many other compounds present in wild mushrooms can also act as antioxidants (reducers), so whole extracts from a wide range of species need to be examined. To gain further knowledge in this area, the relationship between the antioxidant potential (scavenging effect and reducing power) and chemical composition of twenty three samples from seventeen Portuguese wild mushroom species was investigated. A wide range of analytical parameters reported by our research group (including ash, carbohydrates, proteins, fat, monounsaturated fatty acids, polyunsaturated fatty acids, saturated fatty acids, phenolics, flavonoids, ascorbic acid and -carotene) were studied and the data were analysed by partial least squares (PLS) regression analysis to allow correlation of all the parameters. Antioxidant activity correlated well with phenolic and flavonoid contents. A QCAR (Quantitative Composition-Activity Relationships) model was constructed, using the PLS method, and its robustness and predictability were verified by internal and external cross-validation methods. Finally, this model proved to be a useful tool in the prediction of mushrooms’ reducing power.

Phenolic acids determination by HPLC-DAD-ESI/MS in sixteen different portuguese wild mushrooms species

Barros, Lillian; Dueñas, Montserrat; Ferreira, Isabel C.F.R.; Baptista, Paula; Santos-Buelga, Celestino
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
66.51%
Analysis of phenolic compounds in sixteen Portuguese wild mushrooms species has been carried out by high-performance liquid chromatography coupled to photodiode array detector and mass spectrometer (HPLC–DAD–ESI/MS). No flavonoids were detected in the analysed samples, but diverse phenolic acids namely protocatechuic, p-hydroxybenzoic and p-coumaric acids, and two vanillic acid isomers were found and quantified. A related non-phenolic compound, cinnamic acid, was also detected in some samples, being the only compound found in Cantharellus cibarius (14.97 mg/kg, dry matter), Lycoperdon perlatum (14.36 mg/kg) and Macrolepiota procera (21.53 mg/kg). p-Hydroxybenzoic acid was found in the majority of the samples, being the most abundant compound in Agaricus silvicola (238.7 mg/kg). Ramaria botrytis showed the highest phenolic acids concentration (356.7 mg/kg) due to the significant contribution of protocatechuic acid (342.7 mg/kg).

Study and characterization of selected nutrients in wild mushrooms from Portugal by gas chromatography and high performance liquid chromatography

Heleno, Sandrina A.; Barros, Lillian; Sousa, Maria João; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.68%
The present work aims at contributing to the documentation of the nutritional composition of wild mushrooms. Fatty acid and sugar profiles of 10 different Portuguese wild mushrooms were obtained by gas chromatography coupled to a flame ionization detector (GC/FID) and high performance liquid chromatography coupled to a refraction index detector (HPLC/RID), respectively, the latter methodology being then completely validated. The macronutrient profile in general revealed that the wild mushrooms were rich sources of protein (24.32–76.63 g/100 g) and carbohydrates (10.35–55.48 g/100 g), and had low amounts of fat (0.36–2.63 g/ 100 g). The highest energetic contribution was guaranteed by Hygrophoropsis aurantiaca. The analysis of fatty acid composition allowed the quantification of 25 fatty acids. Unsaturated fatty acids and, in particular, oleic and linoleic acids, were predominant (17–61% and 20–54%, respectively). In the analysis of free sugars, all the compounds were separated in a period of time of 10 min; the method used proved to be sensitive, reproducible and accurate. Arabinose (1.53–7.66 g/100 g), mannitol (0.38–18.41 g/100 g) and trehalose (0.21–18.66 g/ 100 g) were the most abundant sugars.

Tocopherols composition of Portuguese wild mushrooms with antioxidant capacity

Heleno, Sandrina A.; Barros, Lillian; Sousa, Maria João; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
66.64%
The antioxidant composition and properties of 18 Portuguese wild mushrooms (Clitocybe alexandri, Cortinarius glaucopus, Fistulina hepatica, Hydnum repandum, Hygrophoropsis aurantiaca, Hypholoma capnoides, Laccaria amethystina, Laccaria laccata, Lactarius aurantiacus, Lactarius salmonicolor, Lepista inversa, Lepista sordida, Mycena rosea, Russula delica, Russula vesca, Suillus collinitus, Suillus mediterraneensis, Tricholoma sulphureum) were evaluated, in order to contribute to the overall characterisation of these products. Their radical-scavenging capacity, reducing power and inhibition of lipid peroxidation measured in liposome solutions was fully studied. Furthermore, the tocopherols composition was determined by HPLC-fluorescence. The analysed mushrooms contain powerful antioxidants such as phenols (0.51– 7.90 mg/g) and tocopherols (0.02–8.04 lg/g). b-Tocopherol was the vitamer detected in higher amounts, while d-tocopherol was not detected in the majority of the samples. All the species proved to have antioxidant activity being more significant for H. aurantiaca (EC50 values lower than 1.35 mg/ml) due to the contribution of antioxidants such as phenols (7.90 mg/g) and tocopherols (0.02–1.94 lg/g). The ongoing research states the nutraceutical potential of all these unique species...

Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines

Vaz, Josiana A.; Heleno, Sandrina A.; Martins, Anabela; Almeida, Gabriela M.; Vasconcelos, M. Helena; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
66.59%
The in vitro antioxidant and growth inhibitory activity of extracts obtained from two Portuguese wild mushrooms (Clitocybe alexandri and Lepista inversa) was studied in human tumour cell lines. The extracts were phenolic (methanolic and ethanolic) and polysaccharidic (boiling water). The antioxidant activity assays included evaluation of radical-scavenging capacity, reducing power and inhibition of lipid peroxidation measured in liposome solutions. Extract-induced cell growth inhibition was measured in four different tumour cell lines (lung, breast, colon and gastric cancer) using the SRB assay. The polysaccharidic extract oft. inversa was the most potent as antioxidant (EC50 < 1.8 +/- 0.1 mg/ml), while the phenolic ethanolic extract of C alexandri was the most potent as inhibitor of growth of the studied cancer cell lines (Gl(50) <26.0 +/- 1.3 mu g/ml). Together, these activities indicate that these mushrooms are promising sources of bioactive compounds. (C) 2010 Elsevier Ltd. All rights reserved.

Phenolic profile of seventeen Portuguese wild mushrooms

Vaz, Josiana A.; Barros, Lillian; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.51%
Analysis of phenolic compounds in seventeen Portuguese wild mushroom species was 26 carried out by high-performance liquid chromatography coupled to photodiode array 27 detection (HPLC-DAD). Protocatechuic, p-hydroxybenzoic, p-coumaric and cinnamic acid 28 were found and quantified. Fistulina hepatica showed the highest phenolic acids 29 concentration (111.72 mg/Kg, dw) due to the significant contribution of protocatechuic 30 (67.62 mg/Kg) and p-hydroxybenzoic (41.92 mg/kg) acids. The edible mushrooms 31 analyzed could be directly used in the human diet to combat oxidative stress, while 32 inedible species could represent a source of extractable phenolic compounds to be used as 33 additives in the food industry or as components in pharmaceutical and cosmetic 34 formulations, due to their well-known antioxidant properties.

Volatile composition of three species of wild mushrooms from Trás-os-Montes region using HS-SPME and GC/IT-MS

Nogueira, Liliana; Malheiro, R.; Pinho, P. Guedes de; Baptista, Paula
Fonte: Universidade de Aveiro Publicador: Universidade de Aveiro
Tipo: Conferência ou Objeto de Conferência
ENG
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66.68%
Trás-os-Montes region is considered one of the richest regions of Portugal concerning the existence of wild mushrooms. In the present work, was studied the volatile profile of three species of wild mushrooms, Hebeloma crustuliniforme, Clitocybe odora and Tricholoma fracticum. These species have different flavours, namely radish-, anise-like odor, and not distinctive or slightly mealy odor, respectively. The volatile fractions were determined in fresh mushrooms by headspace solid-phase microextraction (HS-SPME) combined with gas-chromatography/ion trap-mass spectrometry (GC/IT-MS). Several volatile compounds were identified, belonging to different chemical classes. The three studied mushroom species reported different volatile profiles as presented in Figure 1. Meanwhile these species are mainly composed by alcohols and aldehydes, and in minor content by sesquiterpenes, terpenic compounds, esters, ketones and other chemical classes. 3-Octanol and 1-octanol were identified in the species contributing to their flavour. ρ-Anisaldehyde was only identified in Clitocybe odora and was the main volatile compound identified in this specie. It contributes with the characteristic anise smell of this mushroom, and could be also used as a chemical authenticity marker. Linalool was present in the three species in considerable amounts contributing with floral scents. Overall the volatile profile of the three species contributes with characteristic fragrances...

Biomolecule profiles in inedible wild mushrooms with antioxidant value

Reis, Filipa S.; Pereira, Eliana; Barros, Lillian; Sousa, Maria João; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: MDPI Publicador: MDPI
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
66.59%
The use of natural products isolated from mushrooms, included inedible species, against infection, cancer diseases and other oxidative-stress related diseases is one of the cornerstones of modern medicine. In the present work, the antioxidant molecule profiles of inedible mushroom species were evaluated and compared with those of edible species. The order of antioxidant abundance found in inedible wild mushrooms was: phenolics > flavonoids > ascorbic acid > tocopherols > carotenoids, similar to that of edible species. Furthermore the same energetic biomolecules were found including the disaccharide trehalose, the monosaccharide alcohol derivative mannitol and the fatty acids palmitic, oleic and linoleic acids. Fomitopsis pinicola revealed a very high phenolics concentration (388 mg GAE/g extract) and powerful antioxidant properties, mainly reducing power (EC50 value 60 g/mL similar to the standard Trolox®). It could find applications in the prevention of free radical-related diseases as a source of bioactive compounds.

QCAR models to predict wild mushrooms radical scavenging activity, reducing power and lipid peroxidation inhibition

Froufe, Hugo J.C.; Abreu, Rui M.V.; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
66.68%
Wild mushrooms have become attractive as a source of physiologically beneficial compounds including antioxidants such as phenolic compounds and tocopherols. The concentrations of antioxidant compounds (phenolics and α-tocopherol) and EC50 values of antioxidant activity (concentration required to achieve 50% of radical scavenging activity and lipid peroxidation inhibition, or 0.5 of absorbance in reducing power) were analyzed by partial least square (PLS) regression analysis. Three QCAR (Quantitative Composition-Activity Relationship) models were constructed and their robustness and predictability were verified by internal and external cross-validation methods. Antioxidant activity correlated well with phenolics and -tocopherol contents, the major antioxidants in wild mushrooms. The models proved to be useful tools in the prediction of mushrooms radical scavenging activity, reducing power and lipid peroxidation inhibition.

Phenolic, polysaccharidic and lipidic fractions of mushrooms from northeast Portugal: chemical compounds with antioxidant properties.

Heleno, Sandrina A.; Barros, Lillian; Queiroz, Maria João R.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.53%
Mushrooms do not constitute a significant portion of the human diet, but their consumption continues to increase due to their functional benefits and presence of bioactive compounds. Some of those compounds can be found in the phenolic, polysaccharidic, and lipidic fractions of edible and inedible species. Herein, those fractions of five wild mushrooms (Coprinopsis atramentaria, Lactarius bertillonii, Lactarius vellereus, Rhodotus palmatus, and Xerocomus chrysenteron) from northeastern Portugal were studied for their chemical composition and antioxidant properties. Protocatechuic, p-hydroxybenzoic, p-coumaric, and cinnamic acids were found in the phenolic fraction; rhamnose, xylose, fucose, arabinose, fructose, glucose, manose, mannitol, sucrose, maltose, and trehalose were quantified in polysaccharidic fraction; and linoleic and stearic (only in Lactarius sp.) acids, and β- and γ-tocopherols were the main compounds in the lipidic fraction. C. atramentaria and X. chrysenteron phenolic fractions gave the highest free radical scavenging activity, reducing properties, and lipid peroxidation inhibition in brain homogenates, which is in agreement with its highest content in total phenolics. Furthermore, among the polysaccharidic fractions C. atramentaria also gave the highest antioxidant activity...

Add-value of Lactarius deliciosus and Macrolepiota procera wild mushrooms due to their nutritional and nutraceutical potential.

Fernandes, Ângela; Oliveira, M.B.P.P.; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
56.51%
This study describes the nutritional and nutraceutical potential of two species of wild edible mushrooms (Lactarius deliciosus and Macrolepiota procera) commonly consumed in the region of Trás-os-Montes, Northeast Portugal. The nutritional parameters analyzed were moisture, ash, fat, proteins, carbohydrates and energetic contribution. Free sugars and fatty acids were also determined by high performance liquid chromatography coupled with a refractive index detector (HPLC/RI) and gas chromatography coupled with a flame ionization detector (GC/FID), respectively. Macronutrient profile revealed that the studied species are rich sources of carbohydrates, proteins and energy, revealing low fat content. Mannitol and trehalose were the most abundant sugars in both species. The main fatty acid found in M. procera was linoleic acid, while stearic acid was the most abundant in L. deliciosus. The nutraceutical potential was also evaluated through antioxidant properties measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of LPO using thiobarbituric acid reactive substances (TBARS). The mentioned assays showed and high antioxidant activity for both samples species. Phenolic acids and related compounds were analysed by HPLC coupled to diode array detection (HPLC/DAD) and p-Hydroxybenzoic acid was found in L. deliciosus and cinnamic acid in M. procera.

Study of chemical changes and antioxidant activity variation induced by gamma-irradiation on wild mushrooms: comparative study through principal component analysis

Fernandes, Ângela; Barreira, João C.M.; Antonio, Amilcar L.; Santos, Pedro M.P.; Martins, Anabela; Oliveira, M.B.P.P.; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.63%
Mushrooms are especially sensitive to senescence, browning, water loss and microbial attack. Furthermore, wild species are characterized for their seasonality, demanding the development of suitable preservation technology. Gamma-irradiation was previously tested in wild Lactarius deliciosus, being verified that its application up to 1 kGy did not imply significant changes in chemical parameters. Herein, the effects of higher gamma-irradiation doses, typically used in natural food matrices like fruits or vegetables, were assessed in Boletus edulis Bull.: Fr. and Hydnum repandum L.: Fr. by checking for changes in nutritional parameters, free sugars, tocopherols, fatty acids, organic acids and antioxidant activity indicators. To have representative samples, the used carpophores were collected in different maturity stages, using the same number of specimens for each stage and also for each mushroom species. The specific effects of each tested irradiation were evaluated in an integrated manner through principal component analysis. The correspondent biplots indicate that differences caused by gamma-irradiation are enough to separate irradiated and non-irradiated samples of both mushrooms. Nevertheless, nutritional profiles were not affected in high extension...

Chemical characterization and bioactivity of two different species of wild mushrooms: comparison between Portuguese and Serbian samples

Reis, Filipa S.; Stojković, Dejan; Soković, Marina; Glamočlija, Jasmina; Ćirić, Ana; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
56.59%
Mushrooms are widely appreciated all over the world for their nutritional properties, and also for their pharmacological value. They have been considered valuable health foods, being a source of many different bioactive compounds such as unsaturated fatty acids, tocopherols, organic acids and phenolic compounds. The present work reports and compares the nutritional value, bioactive compounds and antioxidant properties of two wild edible mushroom species from Portugal and Serbia: Boletus aereus and Calocybe gambosa. Regarding the nutritional value of the studied species, carbohydrates were the macronutrients found in higher amounts and no major differences were observed between the energetic values of the samples. Mannitol and trehalose were quantified in all the studied samples and it was also possible to quantify rhamnose and fructose in Boletus aereus from Serbia. Analyzing the fatty acids profile, unsaturated fatty acids predominated over saturated fatty acids. About tocopherols, β-tocopherol was only found in Boletus aereus from Serbia; α-,γ- and δ-isoforms were part of the profile of the Portuguese samples. Protocatechuic acid was only quantified in Calocybe gambosa from Portugal. Two acids, p-hydroxybenzoic and p-coumaric...

Analytical methods applied to the chemical characterization and antioxidant properties of three wild edible mushroom species from Northeastern Portugal

Reis, Filipa S.; Barros, Lillian; Sousa, Maria João; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.63%
The chemical composition and the antioxidant potential of three species of wild mushrooms from Northeastern Portugal, namely Agaricus albertii, Agaricus urinascens var. excellens, and Pleurotus eryngii, were compared. Standard procedures were followed in the nutritional value evaluation, while chromatographic procedures were used to analyze free sugars, fatty acids, tocopherols, phenolic compounds, and organic acids. To assess the antioxidant potential, reducing power, radical-scavenging activity, and lipid peroxidation inhibition were evaluated. P. eryngii revealed the highest levels of macronutrients, except proteins, as also the highest sugars, tocopherols, and monounsaturated fatty acids contents. A. albertii and A. urinascens var. excellens showed similar macronutrients composition. However, A. albertii revealed the highest content in PUFA and phenolic compounds. P. eryngii revealed the highest reducing power and radical-scavenging activity and A. albertii the highest lipid peroxidation inhibition. This study provides a detailed chemical characterization and antioxidant potential evaluation of three species of wild mushrooms from Portugal not yet previously reported. Thus, this work intended to contribute to the increase of information concerning species of edible mushrooms (directed to the scientific community and general population) as well as contribute to the conservation of these resources as sources of compounds of interest.

Volatile biomarkers for wild mushrooms species discrimination

Malheiro, R.; Pinho, P. Guedes de; Soares, Sandra; Ferreira, António César da Silva; Baptista, Paula
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.55%
Secondary metabolites present in the volatile fraction of six wild mushroom species (Clitocybe odora, Clitocybe fragrans, Hebeloma crustuliniforme, Lepista nuda, Tricholoma fracticum and Tricholoma terreum) were studied, as an attempt to identify compounds capable to distinguish mushroom species for taxonomic and authentication purposes. Volatiles were assessed by headspace solid phase microextraction (HS–SPME) and by gas chromatography/ion-trap mass-spectrometry (GC/IT–MS). By using target analysis, 46 volatiles were grouped in 5 chemical classes: alcohols, aldehydes, ketones, sesquiterpene-like compounds and terpenes. Each species revealed a unique volatile profile, with changes in the dominant chemical class. Aliphatic compounds with eight carbon atoms, such as 3-octanol, 1-octanol and 3-octanone were the most abundant. The non-target approach application, using all HS–SPME–GC–MS data (raw chromatograms) aims to detect a large number of compounds to get a fingerprint of each sample. This procedure, involving previous data treatments as chromatogram data alignments, sample data fingerprints, and multivariate analysis, represents a powerful tool to execute an initial screening of the analytical results, enabling a faster interpretability of the results without time-consuming through identifications and quantifications. Unsupervised signal decomposition techniques such as principal component analysis (PCA) applied both to targeted and non-targeted approaches revealed 11 volatile compounds (3-octanol...

Gamma irradiation preserves oleic acid in wild Hydnum repandum L.: Fr.

Fernandes, Ângela; Barreira, João C.M.; Antonio, Amilcar L.; Oliveira, M.B.P.P.; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
56.63%
Oleic acid is an essential fatty acid, omega 9, which participates in the metabolism and plays a key role in the synthesis of hormones. Studies have demonstrated that monounsaturated fatty acids help in lowering the leveis of LDL (low-density lipoprotein), increasing the leveis of HDL (high-density lipoprotein), suppressed appetite and short-term food intake in overweight subjects [1]. Wild mushrooms are excellent to be induded in low caloric diets, presenting higher levels of unsaturated fatty adds than saturated ones [2]. Nevertheless, the high perishability is a common characteristic in mushrooms that leads to lose quality immediately after harvest. In this sense, there are continuous investigations to find an effective consenservation technology. Gamma irradiation has been applied with success in extend the post-harvest of fresh mushrooms [3]. In the present work, the effects of gamma irradiation (1 and 2 kGy) in fresh samples of wild Hydnum repandum L.: Fr. were assessed, regarding the content and profile of fatty acids. The fruiting bodies were collected in Trás-os-Montes (Northeast of Portugal) in November 2012. The irradiation was performed in experimental equipment with four Co-60 sources and fatty acids were analyzed by gas-chromatography coupled to flame ionization detection (GC-FID). The most abundant fatty acids in H. repandum were palmitic acid (C16:0)...

Phenolic, polysaccharidic and lipidic fractions of mushrooms from northeast Portugal : chemical compounds with antioxidant properties

Heleno, Sandrina Alves; Barros, Lillian; Martins, Anabela; Queiroz, Maria João R. P.; Santos-Buelga, Celestino; Ferreira, Isabel C. F. R.
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artigo de Revista Científica
Publicado em 20/04/2012 ENG
Relevância na Pesquisa
56.53%
Mushrooms do not constitute a significant portion of the human diet, but their consumption continues to increase due to their functional benefits and presence of bioactive compounds. Some of those compounds can be found in the phenolic, polysaccharidic and lipidic fractions of edible and inedible species. Herein, those fractions of five wild mushrooms (Coprinopsis atramentaria, Lactarius bertillonii, Lactarius vellereus, Rhodotus palmatus and Xerocomus chrysenteron) from Northeast Portugal were studied for their chemical composition and antioxidant properties. Protocatechuic, p-hydroxybenzoic, p-coumaric and cinnamic acids were found in the phenolic fraction, ramnose, xylose, fucose, arabinose, fructose, glucose, manose, mannitol, sucrose, maltose and trehalose were quantified in polysaccharidic fraction, linoleic and stearic (only in Lactarius sp.) acids, and β- and γ-tocopherols were the main compounds in the lipidic fraction. C. atramentaria and X. chrysenteron phenolic fractions gave the highest free radical scavenging activity, reducing properties and lipid peroxidation inhibition in brain homogenates, which is in agreement with its highest content in total phenolics. Furthermore, among the polysaccharidic fractions C. atramentaria also gave the highest antioxidant activity...

Free radical scavenging and antimicrobial properties of extracts of wild mushrooms

Oyetayo,V.O.
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2009 EN
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66.71%
Antioxidant and antimicrobial potentials of extracts obtained from four wild mushrooms, Termitomyces clypeatus (TCE), Termitomyces robustus (TRE), Lentinus subnudus (LSE) and Lenzites species (LZE) collected in Nigeria were investigated. LSE and LZE displayed good scavenging activity against 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) and ferrous ion radicals at concentration of 2 mg/mL. However, TRE and TCE exhibited better superoxide anion scavenging effect at 2 mg/mL. All extracts (TCE, TRE, LSE and LZE) had comparable scavenging effect on hydroxyl radicals as butylated Hydroxytoluene (BHT) used as control. Moreover, extracts from the wild mushrooms were able to inhibit the growth of all indicator organisms at concentrations between 12.5 mg/mL to 100 mg/mL. LSE and LZE, however, showed better antimicrobial effect on the indicator organisms. The results suggest that extracts obtained from the four wild mushrooms may serve as sources of new bioactive compounds with effective antioxidant and antimicrobial activity.