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Caracterização de variedades minoritárias de vitis vinifera L. da região de Trás-os-Montes e contribuição para o estudo do seu potencial enológico

Costa, Daniela Coelho da
Fonte: Instituto Politécnico de Bragança, Escola Superior Agrária Publicador: Instituto Politécnico de Bragança, Escola Superior Agrária
Tipo: Dissertação de Mestrado
POR
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96.4%
Neste trabalho apresentam-se os resultados de uma prospeção de variedades de Vitis vinífera L., desconhecidas ou ameaçadas de extinção, realizada no nordeste de Portugal (Trás-os-Montes). O conhecimento das castas em quantidade, distribuição pelas regiões e em que características se expressam é de enorme interesse prático, pois a variabilidade só pode ser explorada e conservada se se dispuser de um conhecimento objetivo sobre ela, que acrescente valor e faça uma diferença positiva em relação a outros países ou regiões vitícolas. As castas referenciadas durante os trabalhos de campo, que decorreram nos anos de 2011 e 2012, foram marcadas e foi feita, ao longo do seu ciclo, a sua caracterização ampelográfica utilizando 72 descritores do OIV (Office Internationale de la Vigne et du Vin). Os resultados da prospeção e caracterização permitiram referenciar 29 castas minoritárias sendo a maioria desconhecidas. Analisam-se as características ampelográficas e determinam-se alguns parâmetros dos mostos (pH, peso por bago, índice de polifenóis totais, intensidade da cor, tonalidade da cor, acidez total e os sólidos solúveis totais). No conjunto das castas caracterizadas destacam-se duas com perfil para consumo como uva de mesa e as restantes com indicações de potencial enológico variável que deverá ser confirmado em estudos futuros. This work shows the results of a broad prospection carried out in the northeastern of Portugal (Trás-os-Montes)...

Efeito protetor de fitomedicamento Vitis Vinifera L na lesão renal aguda induzida pelo Tacrolimus; Protective effect of the phytomedicine Vitis Vinifera L in acute kidney injury by Tacrolimus

Silva, Wanessa Teixeira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 04/11/2010 PT
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As lesões renais agudas (LRAs) nefrotóxicas correspondem a 30% dos casos de LRA. A nefrotoxicidade é efeito indesejável de diversos fármacos de uso rotineiro na clínica, entre eles as drogas imunossupressoras. A nefrotoxicidade do Tracolimus (Fk 506) é uma das causas de LRA após o transplante renal. Com a ampla utilização do Fk 506 nas terapias imunossupressoras e devido ao seu potencial nefrotóxico que pode levar à perda do enxerto, o objetivo desse estudo foi avaliar o efeito renoprotetor do extrato de Vitis vinifera L, um fitomedicamento com efeito antiinflamatório e antioxidante, na nefrotoxicidade induzida pelo Tacrolimus em ratos. Foram utilizados ratos Wistar, machos, adultos, pesando entre 250 - 300g, todos tratados 1x/dia por 5 dias, conforme os seguintes grupos: Salina (grupo controle) (NaCl 0,9%, 0,1ml por gavagem); Vitis (Vitis vinifera L 3mg/kg por gavagem), Fk (Tacrolimus 0,5mg/kg por gavagem) e Fk+Vitis (Tacrolimus 0,5mg/kg + 3mg/kg por gavagem). Foram avaliados a função renal (FR) (clearance de creatinina, método Jaffé); os peróxidos urinários (PU) (FOX-2), o malondealdeído (MDA-TBARS) e a histologia renal. Os dados desse estudo confirmaram a lesão nefrotóxica de caráter oxidativo induzida pelo Tacrolimus. A Vitis vinifera L demonstrou efeito renoprotetor significativo...

Phenolic profile of Sercial and Tinta Negra Vitis vinifera L. grape skins by HPLC-DAD-ESI-MSn: Novel phenolic compounds in Vitis vinifera L. grape

Perestrelo, Rosa; Lu, Ying; Santos, Sónia A. O.; Silvestre, Armando J. D.; Neto, Carlos P.; Câmara, José S.; Rocha, Sílvia M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
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This study represents the first phytochemical research of phenolic components of Sercial and Tinta Negra Vitis vinifera L. The phenolic profiles of Sercial and Tinta Negra V. vinifera L. grape skins (white and red varieties, respectively) were established using high performance liquid chromatography-diode array detection-electrospray ionisation tandem mass spectrometry (HPLC-DAD-ESI-MSn), at different ripening stages (veraison and maturity). A total of 40 phenolic compounds were identified, which included 3 hydroxybenzoic acids, 8 hydroxycinnamic acids, 4 flavanols, 5 flavanones, 8 flavonols, 4 stilbenes, and 8 anthocyanins. For the white variety, in both ripening stages, hydroxycinnamic acids and flavonols were the main phenolic classes, representing about 80% of the phenolic composition. For red variety, at veraison, hydroxycinnamic acids and flavonols were also the predominant classes (71%), but at maturity, anthocyanins represented 84% of the phenolic composition. As far as we know, 10 compounds were reported for the first time in V. vinifera L. grapes, namely protocatechuic acid-glucoside, p-hydroxybenzoyl glucoside, caftaric acid vanilloyl pentoside, p-coumaric acid-erythroside, naringenin hexose derivate, eriodictyol-glucoside...

Efeitos da disponibilidade hídrica na fisiologia e produção de vitis vinifera l. Na região demarcada do douro

Sousa, Tiago Pinho Alves de
Fonte: Universidade de Trás-os-Montes e Alto Douro Publicador: Universidade de Trás-os-Montes e Alto Douro
Tipo: Tese de Doutorado
POR
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96.51%
Tese de Doutoramento em Ciências Agronómicas; A temática das relações hídricas na vinha e sobretudo o seu impacto na produção não é consensual. Embora a rega seja uma prática agronómica comum em muitas zonas vitícolas do Globo, continuam a ser levantadas questões acerca das vantagens/inconvenientes que poderão advir da sua utilização. O presente trabalho pretende contribuir para a clarificação do tema e contextualizá-lo nas condições particulares da Região Demarcada do Douro (RDD). Em 2003 foi assim estabelecido um campo experimental na sub-região do Douro Superior numa parcela de Vitis vinífera L. cv. Tinta Roriz enxertada em 1103P, com o objectivo de avaliar o impacto de diferentes regimes hídricos no comportamento fisiológico da videira e as suas consequências no rendimento e composição dos mostos da RDD. Os tratamentos experimentais definidos diferiam quer na quantidade de água aplicada à vinha, quer no período da sua aplicação: não regado (NR), regado da floração ao pintor a 50% da ET (FP1), regado da floração ao pintor a 100% da ET (FP2), regado do pintor à maturação a 50% da ET (PM1), regado do pintor à maturação a 100% da ET (PM2) e regado da floração à maturação a 100% da ET (FM2). Foram utilizados vários métodos de avaliação do estado hídrico da videira...

Chloroplast genome diversity in Portuguese grapevine (Vitis vinifera L.) cultivars.

Castro, Isaura; Pinto-Carnide, Olinda; Ortiz, Jesús María; Martín, Juan Pedro
Fonte: Humana Press Inc Publicador: Humana Press Inc
Tipo: Artigo de Revista Científica
ENG
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Grapevine chloroplast (cp) DNA diversity was analysed for the first time through amplification and digestion of fragments of the large single copy (LSC)region by polymerase chain reaction–restriction fragment length polymorphism methodology and also by amplification of three microsatellite loci, previously described as polymorphic in grapevine. Thirty-eight grapevine cultivars collected mainly in the North of Portugal, including some neglected cultivars, four international cultivars (Chasselas, Muscat of Alexandria, Muscat of Hamburg and Pinot) and Vitis riparia and Vitis rupestris, were used in this study with the main goal of finding out their pDNA diversity and compare the obtained results with previously published data on cultivars from other regions to ascertain their possible origin. Two different alleles were found in each of the three cpSSR loci. Allele variants of the three loci combined in a total of three different haplotypes (A, B and D). The most frequent haplotype, A, was previously reported as the most frequent in Iberian Peninsula and Occidental Europe. Haplotype B was unique to Rabigato, Muscat of Alexandria, V. riparia and V. rupestris. This haplotype was previously proposed to be an ancestral haplotype. Twenty-seven fragments of the LSC region of Vitis vinifera cpDNA were amplified and then digested with HinfI and TaqI restriction enzymes. Polymorphisms were found in the trnT-psbC (TC) and orf184-petA (OA) fragments. In the TC fragment...

Mineral stress affects the cell wall composition og grapevine (Vitis vinifera L.) callus

Fernandes, João C.; Garcia-Angulo, Penélope; Goulão, Luis F.; Acebes, José L.; Amâncio, Sara
Fonte: Universidade Técnica de Lisboa Publicador: Universidade Técnica de Lisboa
Tipo: Artigo de Revista Científica
Publicado em 28/01/2013 ENG
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Versão provisória aceite p. publicação na Plant Science (ISSN 0168-9452)em 19-01-2013; Grapevine (Vitis vinifera L.) is one of the most economically important fruit crops in the world. Deficit in nitrogen, phosphorus and sulfur nutrition impairs essential metabolic pathways. The influence of mineral stress in the composition of the plant cell wall (CW) has received residual attention. Using grapevine callus as a model system, 6-weeks deficiency of those elements caused a significant decrease in growth. Callus CWs were analyzed by Fourier transform infrared spectroscopy (FT-IR), by quantification of CW components and by immunolocalization of CW epitopes with monoclonal antibodies. PCA analysis of FT-IR data suggested changes in the main components of the CW in response to individual mineral stress. Decreased cellulose, modifications in pectin methyl esterification and increase of structural proteins were among the events disclosed by FT-IR analysis. Chemical analyses supported some of the assumptions and further disclosed an increase in lignin content under nitrogen deficiency, suggesting a compensation of cellulose by lignin. Moreover, polysaccharides of callus under mineral deficiency showed to be more tightly bonded to the CW...

Integration of stress produced reactive oxygen species in the stomatal regulation of micropropagated Vitis vinifera L. plantlets impaired in ABA signaling

Vilela, B.J.; Carvalho, L.C.; Amâncio, Sara
Fonte: Landes Bioscience Publicador: Landes Bioscience
Tipo: Artigo de Revista Científica
Publicado em //2008 ENG
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Article Addendum; Reactive oxygen species (ROS) have been repeatedly implicated as cellular second messengers important in the modulation of almost every ontogenic phase of plant development, from seedling to cell death. In all of these processes, ROS production and detoxification are highly regulated and its levels are kept under tight control, in order to achieve the desired effect at the cellular or plant level. Micropropagated Vitis vinifera L. transferred to ex vitro growth under increased irradiance gave an additional insight into ROS signaling by integrating stress produced hydrogen peroxide (H2O2) into normal signaling pathways with distinctive effects critical for the survival, growth and development of these plants. Here we discuss in further detail the relevance of these results and propose a model that may explain this phenomenon

Composition and synthesis of raphide crystals and druse crystals in berries of Vitis vinifera L. cv. Cabernet Sauvignon: Ascorbic acid as precursor for both oxalic and tartaric acids as revealed by radiolabelling studies

DeBolt, S.; Hardie, J.; Tyerman, S.; Ford, C.
Fonte: Australian Society of Viticulture and Oenology Publicador: Australian Society of Viticulture and Oenology
Tipo: Artigo de Revista Científica
Publicado em //2004 EN
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Biomineralisation in the fleshy pericarp of berries of Vitis vinifera L. gives rise to crystals of two distinct forms, viz. raphides and druses, which are found in exocarp and endocarp cells respectively. Druses have generally been considered as crystalline aggregates of calcium oxalate. However, the organic moiety of raphide crystals has been commonly accepted as tartrate, although we have found no analytical data to support that assumption. We now present TEM and X-ray powder diffraction analysis data showing that raphide crystals of V. vinifera berries are composed of calcium oxalate monohydrate. This work also established ascorbic acid as the biosynthetic precursor of both oxalic and tartaric acids. When ascorbic acid labelled with ¹⁴C at position 1 was introduced into berries via the rachis, 21% and 52% of the added radiolabel was recovered as oxalic and tartaric acids respectively. Purified crystals from the radiolabelled grape berries contained approximately 20% of the original radioactivity, further confirming the role of ascorbic acid in oxalic acid biosynthesis. To our knowledge, this is the first evidence to be published on the formation of oxalic and tartaric acids from ascorbic acid via two distinct pathways operating within the same physiological entity (organ).; Seth Debolt...

Direct measurement of hydraulic properties in developing berries of Vitis vinifera L. cv Shiraz and Chardonnay

Tyerman, S.; Tilbrook, J.; Pardo, C.; Kotula, L.; Sullivan, W.; Steudle, E.
Fonte: Australian Society of Viticulture and Oenology Publicador: Australian Society of Viticulture and Oenology
Tipo: Artigo de Revista Científica
Publicado em //2004 EN
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Berries of Vitis vinifera L. cv Shiraz can undergo weight loss during later stages of ripening. Existing published views on how weight loss occurs are based on changes in capacity of the vascular system to import water during development (McCarthy and Coombe, Australian Journal of Grape and Wine Research, 5, 17-21, 1999). One important element of these views is the proposed cessation of water flow through the xylem after veraison. We have now measured the water flow into berries of Shiraz and Chardonnay as they develop using the pressure probe and the high pressure flow meter (HPFM). The pressure probe connected to the pedicel of individual berries provided measurements of single berry hydraulic conductance. By systematic excision of tissue segments of the berry and pedicel we determined where in the pathway hydraulic conductance changed during development. The HPFM was used on whole bunches showing that berries (including pedicels) represent parallel high hydraulic resistances and that the hydraulic resistance of the bunch axis was rather small. The hydraulic conductance per berry could be determined from excision experiments. There was close agreement between the pressure probe and HPFM measurements. Both showed a ten-fold reduction in hydraulic conductance of whole berries from veraison to full ripeness. Shiraz had hydraulic conductances that were 2- to 5-fold higher than those for Chardonnay. Shiraz maintained a higher hydraulic conductance past 90 days after flowering than Chardonnay. The decrease in hydraulic conductance occurred in both the distal and proximal parts of the berry for both varieties. The pressure probe also provided measurements of the xylem pressure that non-transpiring berries could develop. These pressures were -0.2 to -0.1 MPa until veraison and increased to zero when the juice osmotic potential reached about -3 MPa in Chardonnay and -4 MPa in Shiraz. The results suggest values of the reflection coefficient of the osmotic barrier around the xylem vessels of about 0.1-0.2 at veraison decreasing to 0 at harvest. It is suggested that in addition to changes in xylem anatomy...

Development of seed polyphenols in berries from Vitis vinifera L. cv. Shiraz

Kennedy, J.; Troup, G.; Pilbrow, J.; Hutton, D.; Hewitt, D.; Hunter, C.; Ristic, R.; Iland, P.; Jones, G.
Fonte: Australian Society of Viticulture and Oenology Publicador: Australian Society of Viticulture and Oenology
Tipo: Artigo de Revista Científica
Publicado em //2000 EN
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Polyphenols extracted from the seeds of Vitis vinifera L. cv. Shiraz berries were monitored during berry development. Initially seeds were green, plump and had pliable seed coats, but beginning at veraison the seeds browned in colour, became desiccated and the seed coats hardened. Isolated polyphenols consisted of flavan-3-of monomers ((+)-catechin, (-)-epicatechin and (-)-epicatechin-3-O-gallate) and procyanidins. The procyanidins were maximal in the 3 weeks prior to veraison, increasing little during this period. The amounts of flavan-3-ol monomers increased 5-fold during this same period of time, indicating that the procyanidins and the flavan-3-ol monomers accumulate at different stages. Beginning at veraison, amounts of all polyphenols declined and changed in composition. The decrease in amount followed second-order kinetics. Polyphenol changes after veraison could be explained by oxidation and therefore, electron paramagnetic resonance (EPR) spectroscopy was used to follow the potential development of radical species in the developing seeds. Spectra consistent with a phenoxyl radical were observed in the developing seeds. The concentration of radicals remained low until veraison but then increased, reaching a maximum three weeks later...

Concentrações de cloreto de cálcio e tempos de armazenamento nos teores de açúcares redutores de uvas cv red globe (Vitis vinifera L)

Carvalho,Geny Lopes de; Lima,Luiz Carlos de Oliveira; Silva,José Daniel; Siqueira,Heloísa Helena; Morais,Elisa Carvalho
Fonte: Editora da Universidade Federal de Lavras Publicador: Editora da Universidade Federal de Lavras
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2008 PT
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Objetivou-se avaliar a ação do cloreto de cálcio na doçura pós-colheita de uvas 'Red Globe' (Vitis vinifera L) cultivadas em Jales, noroeste do Estado de São Paulo, foram adotados os procedimentos: os cachos foram tratados por imersão durante 10 minutos em soluções CaCl2 0, 1, 2 e 3% e deixados por 30, 60 e 90 dias a uma temperatura de 0ºC a 3ºC, 90+ 3% de UR e 0,2 0,3 m/s de velocidade do ar, após os quais foram deixados em temperatura ambiente (20 a 25ºC), para avaliações aos 0, 3 e 6 dias. O delineamento experimental foi inteiramente casualizado em esquema fatorial 4 x 3 x 3 (concentrações de CaCl2 x tempo de armazenamento refrigerado x tempo de armazenamento em temperatura ambiente) com três repetições. Foram feitas avaliações de açúcares redutores totais (g 100g -1), frutose (g 100g -1) e glicose (g 100g -1). Os teores de açúcares redutores totais, frutose e glicose dos frutos variaram com a aplicação de CaCl2, tempos de armazenamento a frio e em temperatura ambiente. Destaca-se a frutose cujos teores em todas as concentrações de CaCl2 (1, 2 e 3%), aos 30 e 60 dias de armazenamento refrigerado e 3 e 6 dias em temperatura ambiente, foram superiores aos teores dos frutos controle. Conclui-se que tratamentos com CaCl2 propicia às uvas melhor sabor...

Volatile organic compounds characterized from grapevine (Vitis vinifera L. cv. Malbec) berries increase at pre-harvest and in response to UV-B radiation

Gil, Mariana; Bottini, Ambrosio Ruben; Berli, Federico Javier; Pontin, Mariela Ana; Silva, Maria Fernanda; Piccoli, Patricia Noemi
Fonte: Elsevier Publicador: Elsevier
Tipo: info:eu-repo/semantics/article; info:ar-repo/semantics/artículo; info:eu-repo/semantics/publishedVersion Formato: application/pdf
ENG
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Ultraviolet-B solar radiation (UV-B) is an environmental signal with biological effects in plant tissues. Recent investigations have assigned a protective role of volatile organic compounds (VOCs) in plant tissues submitted to biotic and abiotic stresses. This study investigated VOCs in berries at three developmental stages (veraison, pre-harvest and harvest) of Vitis vinifera L. cv. Malbec exposed (or not) to UVB both, in in vitro and field experiments. By Head Space-Solid Phase Micro Extraction–Gas Chromatography–Electron Impact Mass Spectrometry (HS-SPME–GC–EIMS) analysis, 10 VOCs were identified at all developmental stages: four monoterpenes, three aldehydes, two alcohols and one ketone. Monoterpenes increased at pre-harvest and in response to UV-B in both, in vitro and field conditions. UV-B also augmented levels of some aldehydes, alcohols and ketones. These results along with others from the literature suggest that UV-B induce grape berries to produce VOCs (mainly monoterpenes) that protect the tissues from UV-B itself and other abiotic and biotic stresses, and could affect the wine flavor. Higher emission of monoterpenes was observed in the field experiments as compared in vitro, suggesting the UV-B/PAR ratio is not a signal in itself.; Fil: Gil...

Comparative Study of the Phenolic Composition of Seeds and Skins from Carme´n ere and Cabernet Sauvignon Grape Varieties (Vitis vinifera L.) during Ripening

Laureano, Olga; Zamora Marín, Fernando; López Solís, Remigio; Peña Neira, Alvaro; Da Silva, Jorge M. Ricardo; Obreque Slier, Elías
Fonte: Universidade do Chile Publicador: Universidade do Chile
Tipo: Artículo de revista
EN
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The phenolic composition of skins and seeds from Vitis vinifera L. cv. Carme´n ere and Cabernet Sauvignon grapes during ripening was evaluated by high-performance liquid chromatography-diode array detection and spectrophotometric analysis. As compared to Cabernet Sauvignon grape skins, Carme´n ere grape skins presented higher contents of total anthocyanins, monomeric flavan-3-ols, and total flavonoids, a higher mean degree of polymerization, a higher percentage of galloylation, a higher average molecular weight of the flavanol fraction, and a higher color intensity. As compared to Cabernet Sauvignon grape seeds, Carme´n ere grape seeds presented a lower content of monomeric flavan-3-ols, a higher mean degree of polymerization, a higher percentage of galloylation, a higher average molecular weight of the flavanol fraction, a lower content of (þ)-catechin, and higher contents of (-)-epicatechin, epicatechin-3-O-gallate, gallic acid, and dimeric procyanidins esterified with gallic acid. Altogether, we conclude that Carme´n ere grapes present a differential composition and evolution of phenolic compounds when compared to Cabernet Sauvignon grapes.; This study was partially supported by Grants Corfo-INNOVA TecnoVid-05CTE02- 04...

Phenolic profile of Sercial and Tinta Negra Vitis vinifera L. grape skins by HPLC–DAD–ESI-MSn: novel phenolic compounds in Vitis vinifera L. grape

Perestrelo, Rosa; Lu, Ying; Santos, Sónia A. O.; Silvestre, Armando J. D.; Neto, Carlos P.; Câmara, José S.; Rocha, Sílvia M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /11/2012 ENG
Relevância na Pesquisa
96.6%
This study represents the first phytochemical research of phenolic components of Sercial and Tinta Negra Vitis vinifera L. The phenolic profiles of Sercial and Tinta Negra V. vinifera L. grape skins (white and red varieties, respectively) were established using high performance liquid chromatography–diode array detection–electrospray ionisation tandem mass spectrometry (HPLC–DAD–ESI-MSn), at different ripening stages (véraison and maturity). A total of 40 phenolic compounds were identified, which included 3 hydroxybenzoic acids, 8 hydroxycinnamic acids, 4 flavanols, 5 flavanones, 8 flavonols, 4 stilbenes, and 8 anthocyanins. For the white variety, in both ripening stages, hydroxycinnamic acids and flavonols were the main phenolic classes, representing about 80% of the phenolic composition. For red variety, at véraison, hydroxycinnamic acids and flavonols were also the predominant classes (71%), but at maturity, anthocyanins represented 84% of the phenolic composition. As far as we know, 10 compounds were reported for the first time in V. vinifera L. grapes, namely protocatechuic acid-glucoside, p-hydroxybenzoyl glucoside, caftaric acid vanilloyl pentoside, p-coumaric acid-erythroside, naringenin hexose derivate, eriodictyol-glucoside...

Solid phase microextraction as a reliable alternative to conventional extraction techniques to evaluate the pattern of hydrolytically released components in Vitis vinifera L. grapes

Perestrelo, Rosa; Caldeira, Michael; Câmara, José S.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /06/2012 ENG
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96.41%
In present research, headspace solid-phase microextraction (HS-SPME) followed by gas chromatography–mass spectrometry (GC–qMS), was evaluated as a reliable and improved alternative to the commonly used liquid–liquid extraction (LLE) technique for the establishment of the pattern of hydrolytically released components of 7 Vitis vinifera L. grape varieties, commonly used to produce the world-famous Madeira wine. Since there is no data available on their glycosidic fractions, at a first step, two hydrolyse procedures, acid and enzymatic, were carried out using Boal grapes as matrix. Several parameters susceptible of influencing the hydrolytic process were studied. The best results, expressed as GC peak area, number of identified components and reproducibility, were obtained using ProZym M with b-glucosidase activity at 35 °C for 42 h. For the extraction of hydrolytically released components, HS-SPME technique was evaluated as a reliable and improved alternative to the conventional extraction technique, LLE (ethyl acetate). HS-SPME using DVB/CAR/PDMS as coating fiber displayed an extraction capacity two fold higher than LLE (ethyl acetate). The hydrolyzed fraction was mainly characterized by the occurrence of aliphatic and aromatic alcohols...

Optimisation of solid-phase microextraction combined with gas chromatography–mass spectrometry based methodology to establish the global volatile signature in pulp and skin of Vitis vinifera L. grape varieties

Perestrelo, Rosa; Barros, António S.; Rocha, Sílvia M.; Câmara, José S.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /09/2011 ENG
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The volatiles (VOCs) and semi-volatile organic compounds (SVOCs) responsible for aroma are mainly present in skin of grape varieties. Thus, the present investigation is directed towards the optimisation of a solvent free methodology based on headspace-solid-phase microextraction (HS-SPME) combined with gas chromatography–quadrupole mass spectrometry (GC–qMS) in order to establish the global volatile composition in pulp and skin of Bual and Bastardo Vitis vinifera L. varieties. A deep study on the extraction-influencing parameters was performed, and the best results, expressed as GC peak area, number of identified compounds and reproducibility, were obtained using 4 g of sample homogenised in 5 mL of ultra-pure Milli-Q water in a 20 mL glass vial with addition of 2 g of sodium chloride (NaCl). A divinylbenzene/carboxen/polydimethylsiloxane fibre was selected for extraction at 60 °C for 45 min under continuous stirring at 800 rpm. More than 100 VOCs and SVOCs, including 27 monoterpenoids, 27 sesquiterpenoids, 21 carbonyl compounds, 17 alcohols (from which 2 aromatics), 10 C13 norisoprenoids and 5 acids were identified. The results showed that, for both grape varieties, the levels and number of volatiles in skin were considerably higher than those observed in pulp. According to the data obtained by principal component analysis (PCA)...

Carpometria da grainha na identificação de ancestraissel vagens de Vitis vinifera L.

Cunha,J.; Cunha,J. P.; Carneiro,L. C.; Fevereiro,Pedro; Eiras-Dias,J.E.
Fonte: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional) Publicador: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2007 PT
Relevância na Pesquisa
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No presente trabalho, são estudadas as medidas da grainha como método para descriminar variedades cultivadas de Vitis vinifera ssp vinifera e plantas de Vitis vinifera ssp sylvestris.Caracterizaram-se ao nível da grainha 10 plantas femininas produtoras de bagos das populações selvagens, 61 variedades de videiratradicionalmente utilizadas em Portugal e 10 variedades internacionalmente conhecidas.O presente trabalho permite concluir que a razão largura/comprimento é o parâmetro que melhor descrimina as plantas em estudo. O métododesenvolvido pode vir a ser útil em estudos de achados de jazidas arqueológicas dado ser um método não destrutivo.Observou-se que as variedades Ferral (50104), Olho de Lebre (51109) e Uva Rei (50713) apresentam um quociente entre 0,65=QC=0,75, oque adverte para a hipótese de parentesco entre estas e as sylvestris.Estes aspectos justificam o parâmetro largura da grainha, um dado passaporte, para aplicação do quociente, no código de caracteresdescritivos de variedades e espécies de Vitis do OIV.

Comparação ampelométrica de populações selvagens de Vitis Vinífera L. e de castas antigas do Sul de Portugal

Coelho,I.; Cunha,J.; Cunha,J.P.; Carneiro,L. C.; Castro,R.; Dias,J. E. Eiras
Fonte: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional) Publicador: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2004 PT
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96.49%
Neste estudo pretende-se analisar as semelhanças existentes entre 11 castas antigas cultivadas no Alentejo e 3 populações de Vitis vinifera spp sylvestris (Gmel) Hegi recentemente detectadas na mesma região, usando métodos ampelometricos. Assim, caracterizaram-se as castas e as populações selvagens através de medições lineares na folha adulta. Para a comparação entre as duas subespécies de Vitis vinifera L. foram utilizados métodos de taxonomia numérica. A comparação entre as castas cultivadas e as videiras selvagens mostrou que é possível distinguilas através dos parametros utilizados na caracterização ampelométrica. Esta distinção decorre principalmente no recorte da folha e no tamanho do lóbulo C, consequentemente no grau de abertura do seio peciolar. Verificou-se também que a casta Marufo apresenta maior semelhança com as plantas da população silvestre de Castelo Branco do que com as castas cultivadas. Pode ainda concluir-se que, as videiras sylvestris possuem seio peciolar mais aberto e folhas mais pequenas que as variedades cultivadas.

Os bosques ribeirinhos, fonte de diversidade genética de Vitis vinifera L.

Cunha,J.; Cunha,J. P.; Lousã,M.; Eiras-Dias,J.E.
Fonte: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional) Publicador: INIAV - DOIS PORTOS (Ex-Estação Vitivinícola Nacional)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2004 PT
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96.4%
A elevada biodiversidade da fauna e da flora que integram os bosques ripícolas, bem como o seu contributo para a regulação física do meio, estabilização da geomorfologia, controlo das características hidrológicas dos sistemas fluviais e descontaminação das águas, conferem-lhes um importante papel no equilíbrio ecológico dos ecossistemas necessários à vida humana. Apesar disso, assiste-se a um quase total desconhecimento de algumas espécies aí presentes e de factores com interesse agronómico a elas associadas, como a rusticidade proveniente de gerações muito longínquas. Este trabalho constitui o primeiro contributo científico e técnico dos bosques ripícolas como suporte de diversidade biológica da espécie Vitis vinifera L., nomeadamente da subespécie espontânea (sylvestris).

Separation and purification of four stilbenes from Vitis vinifera L. cv. cabernet sauvignon roots through high-speed counter-current chromatography

Wei,Yangji; Li,Pi; Ma,Liyan; Li,Jingming
Fonte: South African Journal of Enology and Viticulture Publicador: South African Journal of Enology and Viticulture
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2014 EN
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A method for preparative separation and purification of trans-resveratrol, δ-viniferin, ε-viniferin and trans-vitisin B from the roots of Vitis vinifera L. cv. Cabernet Sauvignon was successfully established and is reported on in this paper. The four important stilbenes were purified by high-speed counter-current chromatography (HSCCC) with a suitable quaternary solvent system composed of chloroform-methanol-n-butanol-water (4:3:0.05:2, v/v). A total of 7.1 mg ± 0.2 mg of trans-resveratrol, 1.1 mg ± 0.1 mg of á-viniferin, 18.7 mg ± 0.5 mg of ε-viniferin, and 12.2 mg ± 0.2 mg of trans-vitisin B, with purities of 97.89%, 90.61%, 94.37% and 78.38% respectively, were obtained from 241 mg of crude sample in a one-step HSCCC separation. The chemical structures of trans-resveratrol and á-viniferin were further confirmed with the retention time using the method of standard addition, while the structural identification of ε-viniferin and trans-vitisin B was performed with LC-ESI/MS, Ή-NMR, and 13C-NMR.