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Previsão de chuva com auxílio de radar de tempo visando a um sistema de alerta antecipado de cheias em áreas urbanas; Precipitation forecast aided by weather radar for early warning system of urban floods

Gonçalves, Micheli Fernandes
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 19/06/2009 PT
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Para reduzir as perdas humanas e materiais durante as inundações, é possível realizar estudo conciso da previsão de chuva, etapa principal de um sistema de alerta antecipado de inundação. O uso de informações de radar de tempo, quando acopladas a modelos de previsão de precipitação baseados fisicamente, pode contribuir para o monitoramento e previsão de episódios de chuva intensa. Desta forma, a previsão de chuva, baseada no uso de informações de radar, juntamente com um modelo conceitual de previsão hidrometeorológica, foi descrita neste trabalho. Teve-se por objetivo aperfeiçoar as previsões de chuva de curtíssimo prazo (poucos minutos), que acopladas a um modelo chuva-vazão, podem ser usadas em sistemas de alerta antecipado. O modelo hidrometeorológico adotado, que considera uma nuvem hipotética unidimensional vertical, foi inicialmente desenvolvido por Georgakakos e Bras (1984a) e ampliado, neste trabalho. Para tal, adotou-se o uso das informações de Topo dos Ecos para determinação da altura das nuvens e considerou-se que a componente do modelo relativa à massa de água líquida no interior da nuvem corresponde à estimativa do conteúdo de água líquida integrado verticalmente (VIL) efetuada por radar. Para eventos de natureza frontal quente e convectiva...

Human Herpesvirus 8 Interleukin-6 (vIL-6) Signals through gp130 but Has Structural and Receptor-Binding Properties Distinct from Those of Human IL-6

Wan, Xiaoyu; Wang, Hailin; Nicholas, John
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /10/1999 EN
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Human herpesvirus 8 (HHV-8) has been associated with classical, endemic (African), and AIDS-related Kaposi’s sarcoma (KS), body cavity-based primary effusion lymphomas, and multicentric Castleman’s disease (MCD). HHV-8 encodes a functional homologue of interleukin-6 (IL-6), a cytokine that promotes the growth of KS and myeloma cells and is found at elevated levels in MCD lesions and patient sera. We have previously reported that the viral IL-6 (vIL-6) gene product can support the growth of the IL-6-dependent murine hybridoma cell line, B9, and that the gp80 (IL-6 receptor [IL-6R]) component of the IL-6 receptor-signal transducer (gp180) complex plays a role in mediating this activity. However, it has been shown by others that vIL-6 can function in human cells independently of IL-6R. Here we have extended our functional studies of vIL-6 by identifying transcription factors and pathways used in human Hep3B cells, investigating the utilization of gp130 and IL-6R by vIL-6, and undertaking mutational analyses of vIL-6 and gp130. The data presented here establish that vIL-6, in common with its endogenous counterparts, can mediate signal transduction through gp130 and activate multiple transcription factors, map residues within the vIL-6 protein that are and are not important for vIL-6 signalling...

Structural Requirements for gp80 Independence of Human Herpesvirus 8 Interleukin-6 (vIL-6) and Evidence for gp80 Stabilization of gp130 Signaling Complexes Induced by vIL-6

Chen, Daming; Nicholas, John
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /10/2006 EN
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Human herpesvirus 8 interleukin-6 (vIL-6) displays 25% amino acid identity with human IL-6 (hIL-6) and shares an overall four-helix-bundle structure and gp130-mediated STAT/mitogen-activated protein kinase signaling with its cellular counterpart. However, vIL-6 is distinct in that it can signal through gp130 alone, in the absence of the nonsignaling gp80 α-subunit of the IL-6 receptor. To investigate the structural requirements for gp80 independence of vIL-6, a series of expression vectors encoding vIL-6/hIL-6 chimeric and site-mutated IL-6 proteins was generated. The replacement of hIL-6 residues with three vIL-6-specific tryptophans implicated in gp80 independence from crystallographic studies or the A and C helices containing these residues did not confer gp80 independence to hIL-6. The N- and C-terminal regions of vIL-6 could be substituted with hIL-6 sequences with the retention of gp80-independent signaling, but substitutions of other regions of vIL-6 (helix A, A/B loop, helix B, helix C, and proximal half of helix D) with equivalent sequences of hIL-6 abolished gp80 independence. Interestingly, the B helix of vIL-6 was absolutely required for gp80 independence, despite the fact that this region contains no receptor-binding residues. Point mutational analysis of helix C...

Signal Transduction by Human Herpesvirus 8 Viral Interleukin-6 (vIL-6) Is Modulated by the Nonsignaling gp80 Subunit of the IL-6 Receptor Complex and Is Distinct from Signaling Induced by Human IL-6▿

Hu, Fang; Nicholas, John
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
EN
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Human herpesvirus 8 (HHV-8) viral interleukin-6 (vIL-6) mediates signaling through the gp130 signal transducer but unlike human IL-6 (hIL-6) does not require the nonsignaling gp80 α subunit of the IL-6 receptor complex. By utilizing a gp80-refractory vIL-6 variant, vIL-6(R189L), we found that signal transduction, as measured by STAT1 and STAT3 activation and gp130 tyrosine phosphorylation in gp80+/gp130+ HEK293T cells, was modulated by gp80. Furthermore, the signaling and BAF-130 cell growth-promoting activities of vIL-6 and hIL-6 could be distinguished, and exogenous addition of soluble gp80 enhanced cell growth supported by vIL-6. Our findings demonstrate that gp80 can modulate vIL-6 activity and that vIL-6 and hIL-6 signaling are not directly equivalent.

HIV-1 Tat Promotes Kaposi’s Sarcoma-Associated Herpesvirus (KSHV) vIL-6-Induced Angiogenesis and Tumorigenesis by Regulating PI3K/PTEN/AKT/GSK-3β Signaling Pathway

Zhou, Feng; Xue, Min; Qin, Di; Zhu, Xiaofei; Wang, Cong; Zhu, Jianzhong; Hao, Tingting; Cheng, Lin; Chen, Xiuying; Bai, Zhiqiang; Feng, Ninghan; Gao, Shou-Jiang; Lu, Chun
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 02/01/2013 EN
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Kaposi’s sarcoma (KS)-associated herpesvirus (KSHV) is etiologically associated with KS, the most common AIDS-related malignancy. KS is characterized by vast angiogenesis and hyperproliferative spindle cells. We have previously reported that HIV-1 Tat can trigger KSHV reactivation and accelerate Kaposin A-induced tumorigenesis. Here, we explored Tat promotion of KSHV vIL-6-induced angiogenesis and tumorigenesis. Tat promotes vIL-6-induced cell proliferation, cellular transformation, vascular tube formation and VEGF production in culture. Tat enhances vIL-6-induced angiogenesis and tumorigenesis of fibroblasts and human endothelial cells in a chicken chorioallantoic membrane (CAM) model. In an allograft model, Tat promotes vIL-6-induced tumorigenesis and expression of CD31, CD34, SMA, VEGF, b-FGF, and cyclin D1. Mechanistic studies indicated Tat activates PI3K and AKT, and inactivates PTEN and GSK-3β in vIL-6 expressing cells. LY294002, a specific inhibitor of PI3K, effectively impaired Tat’s promotion of vIL-6-induced tumorigenesis. Together, these results provide the first evidence that Tat might contribute to KS pathogenesis by synergizing with vIL-6, and identify PI3K/AKT pathway as a potential therapeutic target in AIDS-related KS patients.

Aparato y procedimiento para determinar autom??ticamente la iluminancia del flujo luminoso producido por los focos de un autom??vil

Carn??r i Bad??a, Albert
Fonte: Madrid: Oficina espa??ola de patentes y marcas Publicador: Madrid: Oficina espa??ola de patentes y marcas
Tipo: Patente
ES
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N??mero de publicaci??n: 2 233 165 N??mero de solicitud: 200300416; Aparato y procedimiento para determinar autom??ticamente la iluminancia del flujo luminoso producido por los focos de un autom??vil. Aparato para determinar automaticamente la iluminancia del flujo luminoso producido por los focos de un autom??vil que comprende un robot con un medidor de iluminancia que se desplaza por una carretera, medios de posicionamiento del robot y un dispositivo inal??mbrico de transmisi??n de datos a un ordenador que toma los datos, los procesa y dibuja unas curvas de iluminancia. Procedimiento para determinar la iluminancia de los focos de un autom??vil.

Escudos y blasones en el autom??vil

Fuentes-Mart??n, Jos?? Miguel
Fonte: Universidade de Granada Publicador: Universidade de Granada
Tipo: Parte de Livro
SPA
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Las marcas de autom??viles han utilizado y utilizan escudos y blasones como distintivo de sus productos. Estos var??an en la forma seg??n su origen, y su representaci??n evoluciona partiendo de los escudos de armas de los pa??ses o regiones y la influencia del estilo de moda en el que fueron dibujados y dise??ados.The cars brands have used and used shields and blazons as a distinctive of their products. These vary in form according to their origin, and its representation evolves based of coats of arms of countries or regions and the influence of fashion style in which they were drawn and designed; The cars brands have used and used shields and blazons as a distinctive of their products. These vary in form according to their origin, and its representation evolves based of coats of arms of countries or regions and the influence of fashion style in which they were drawn and designed.

Aplicaciones de los smartphones y la web m??vil en la ciencia y la investigaci??n

Torres-Salinas, Daniel
Fonte: Anuario Thinkepi Publicador: Anuario Thinkepi
Tipo: Artigo de Revista Científica
SPA
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Una de las tendencias de internet con mayor auge es el acceso a la informaci??n a trav??s dispositivos m??viles y especialmente desde smartphones o tel??fonos inteligentes. La denominada web m??vil est?? afectando a todos lo ??mbitos, incluido el de la actividad cient??fica, que se est?? mostrando muy vers??til en su utilizaci??n. Por ello el objetivo de este trabajo es ilustrar el uso que la comunidad cient??fica est?? haciendo de los mismos a trav??s de dos bloques. En el primero nos detenemos en su uso como fuente de informaci??n y referencia, analiz??ndose para ello las principales aplicaciones m??viles de editores de revistas cient??ficas, bases de datos y gestores de referencias. En el segundo bloque se analizan los tel??fonos inteligentes desde el punto de vista de su utilidad como herramienta de investigaci??n y como medio de recolecci??n de datos, entre otros.

Atenci??n sanitaria emergente al usuario discapacitado mediante las nuevas tecnolog??as de la comunicaci??n

Sotillo Hidalgo, Ricardo; Mart??n Castro, Carmen; G??mez Jim??nez, Francisco Javier; Castillo Garz??n, Manuel J.; Mac??as Rodr??guez, Mar??a Fermina
Fonte: Fundaci??n eSalud Publicador: Fundaci??n eSalud
Tipo: Artigo de Revista Científica
SPA
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El objetivo de este trabajo ha sido desarrollar y validar un protocolo basado en comunicaci??n gr??fica y empleo del m??vil que permita a las personas con problemas para la comunicaci??n verbal el acceso al Sistema de Emergencias Sanitarias 061 de Andaluc??a (Espa??a). MATERIAL Y METODO Bas??ndonos en los modelos de cuestionarios que ya tiene implantados el servicio del 061 para env??o y recepci??n de fax por parte de la poblaci??n con discapacidad auditiva, se dise??ar??n unas plantillas que se pueden almacenar en la memoria del tel??fono m??vil y que una vez rellenas se pueden enviar v??a SMS. Se dispone de una plantilla general con los datos de la persona concreta para insertar en todos los mensajes, una plantilla de cuestiones comunes a todos los supuestos cl??nicos y una plantilla para cada supuesto determinado. Estas plantillas se les enviar??n por mensaje SMS a todas las personas que se dan de alta para poder acceder a este servicio. De esta manera las almacenan en sus tel??fonos m??viles para poder hacer uso de ellas en caso de encontrarse en situaci??n de emergencia. RESULTADOS Se recibieron 26 plantillas en forma de SMS, de las cuales 1 estaba sin rellenar, concretamente la primera. En 6 de esos casos no se envi?? imagen solamente la plantilla rellena. Y s??lo en uno de los casos se envi?? la plantilla rellena y la fotograf??a mucho m??s tarde. La respuesta con el consejo sanitario correspondiente en forma de MMS tiene una demora media de 1...

Dise??o y construcci??n de un sistema de transporte de equipos port??tiles para el Centro de Inform??tica de la Universidad EAFIT denominado "Aula M??vil"

Siegert L??pez, Jonnathan
Fonte: Universidad EAFIT; Ingenier??a de Dise??o de Producto; Escuela de Ingenier??a. Departamento de Ingenier??a de Dise??o de Producto Publicador: Universidad EAFIT; Ingenier??a de Dise??o de Producto; Escuela de Ingenier??a. Departamento de Ingenier??a de Dise??o de Producto
Tipo: bachelorThesis; Trabajo de grado; acceptedVersion
SPA
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D??a a d??a est?? m??s presente la tecnolog??a en nuestra cultura, y nunca se queda quieta la evoluci??n, es por este motivo que se debe buscar nuevas formas para hacer que estos desarrollos sean m??s llevaderos, ya que cada creaci??n hace que sea necesario nuevos artefactos para complementar estas nuevas creaciones -- Con este trabajo se busca contribuir a la soluci??n de un problema m??dico que fatiga a los empleados del Centro de Inform??tica de la Universidad EAFIT, con el desplazamiento accionado por la fuerza humana de un veh??culo que transporta 16 computadores port??tiles de un punto A hasta un punto B y viceversa, por todo el campus -- La soluci??n propuesta en este trabajo busca optimizar los tiempos de entrega de los equipos en las aulas de clase, aumentar la capacidad de almacenamiento del veh??culo para extender el n??mero de equipos por clase en un solo viaje, contribuir en la soluci??n de los problemas m??dicos que aquejan a los empleados del Centro de Inform??tica de la Universidad EAFIT, haciendo que el desplazamiento del veh??culo sea el??ctrico y por ultimo este ser?? una seguridad para los equipos de c??mputo ya que se encuentran dentro de un compartimiento en el cual los equipos est??n asegurados individualmente...

Overhaul de una planta de trituraci??n m??vil

Mej??a Escobar, David
Fonte: Universidad EAFIT; Ingenier??a Mec??nica; Escuela de Ingenier??a. Departamento de Ingenier??a Mec??nica Publicador: Universidad EAFIT; Ingenier??a Mec??nica; Escuela de Ingenier??a. Departamento de Ingenier??a Mec??nica
Tipo: bachelorThesis; Trabajo de grado; acceptedVersion
SPA
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The EBV Immunoevasins vIL-10 and BNLF2a Protect Newly Infected B Cells from Immune Recognition and Elimination

Jochum, Simon; Moosmann, Andreas; Lang, Stephan; Hammerschmidt, Wolfgang; Zeidler, Reinhard
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
EN
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Lifelong persistence of Epstein-Barr virus (EBV) in infected hosts is mainly owed to the virus' pronounced abilities to evade immune responses of its human host. Active immune evasion mechanisms reduce the immunogenicity of infected cells and are known to be of major importance during lytic infection. The EBV genes BCRF1 and BNLF2a encode the viral homologue of IL-10 (vIL-10) and an inhibitor of the transporter associated with antigen processing (TAP), respectively. Both are known immunoevasins in EBV's lytic phase. Here we describe that BCRF1 and BNLF2a are functionally expressed instantly upon infection of primary B cells. Using EBV mutants deficient in BCRF1 and BNLF2a, we show that both factors contribute to evading EBV-specific immune responses during the earliest phase of infection. vIL-10 impairs NK cell mediated killing of infected B cells, interferes with CD4+ T-cell activity, and modulates cytokine responses, while BNLF2a reduces antigen presentation and recognition of newly infected cells by EBV-specific CD8+ T cells. Together, both factors significantly diminish the immunogenicity of EBV-infected cells during the initial, pre-latent phase of infection and may improve the establishment of a latent EBV infection in vivo.

Marek's Disease Virus (MDV) Encodes an Interleukin-8 Homolog (vIL-8): Characterization of the vIL-8 Protein and a vIL-8 Deletion Mutant MDV†

Parcells, Mark S.; Lin, Su-Fang; Dienglewicz, Robert L.; Majerciak, Vladimir; Robinson, Dan R.; Chen, Hua-Chien; Wu, Zining; Dubyak, George R.; Brunovskis, Peter; Hunt, Henry D.; Lee, Lucy F.; Kung, Hsing-Jien
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /06/2001 EN
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Chemokines induce chemotaxis, cell migration, and inflammatory responses. We report the identification of an interleukin-8 (IL-8) homolog, termed vIL-8, encoded within the genome of Marek's disease virus (MDV). The 134-amino-acid vIL-8 shares closest homology to mammalian and avian IL-8, molecules representing the prototype CXC chemokine. The gene for vIL-8 consists of three exons which map to the BamHI-L fragment within the repeats flanking the unique long region of the MDV genome. A 0.7-kb transcript encoding vIL-8 was detected in an n-butyrate-treated, MDV-transformed T-lymphoblastoid cell line, MSB-1. This induction is essentially abolished by cycloheximide and herpesvirus DNA polymerase inhibitor phosphonoacetate, indicating that vIL-8 is expressed with true late (γ2) kinetics. Baculovirus-expressed vIL-8 was found to be secreted into the medium and shown to be functional as a chemoattractant for chicken peripheral blood mononuclear cells but not for heterophils. To characterize the function of vIL-8 with respect to MDV infection in vivo, a recombinant MDV was constructed with a deletion of all three exons and a soluble-modified green fluorescent protein (smGFP) expression cassette inserted at the site of deletion. In two in vivo experiments...

Detection of Direct Binding of Human Herpesvirus 8-Encoded Interleukin-6 (vIL-6) to both gp130 and IL-6 Receptor (IL-6R) and Identification of Amino Acid Residues of vIL-6 Important for IL-6R-Dependent and -Independent Signaling

Li, Hong; Wang, Hailin; Nicholas, John
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /04/2001 EN
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27.97%
Human herpesvirus 8 (HHV-8) is associated with Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease; in all of these diseases, interleukin-6 (IL-6) has been implicated as a likely mitogenic and/or angiogenic factor. HHV-8 encodes a homologue of IL-6 (viral IL-6 [vIL-6]) that has been shown to be biologically active in several assays and whose activities mirror those of its mammalian counterparts. Like these proteins, vIL-6 mediates its effects through the gp130 signal transducer, but signaling is not dependent on the structurally related IL-6 receptor (IL-6R; gp80) subunit of the receptor-signal transducer complex. However, as we have shown previously, IL-6R can enhance vIL-6 signal transduction and can enable signaling through a gp130 variant (gp130.PM5) that is itself unable to support vIL-6 activity, indicating that IL-6R can form part of the signaling complex. Also, our analysis of a panel of vIL-6 mutants in transfection experiments in Hep3B cells (that express IL-6R and gp130) showed that most were able to function normally in this system. Here, we have used in vitro vIL-6–receptor binding assays to demonstrate direct binding of vIL-6 to both gp130 and IL-6R and vIL-6-induced gp130–IL-6R complex formation...

Abrogation of Viral Interleukin-6 (vIL-6)-Induced Signaling by Intracellular Retention and Neutralization of vIL-6 with an Anti-vIL-6 Single-Chain Antibody Selected by Phage Display

Kovaleva, Marina; Bussmeyer, Ingo; Rabe, Björn; Grötzinger, Joachim; Sudarman, Enge; Eichler, Jutta; Conrad, Udo; Rose-John, Stefan; Scheller, Jürgen
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /09/2006 EN
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Human herpesvirus 8 (HHV-8) encodes several putative oncogenes, which are homologues to cellular host genes known to function in cell cycle regulation, control of apoptosis, and cytokine signaling. Viral interleukin (vIL-6) is believed to play an important role in the pathogenesis of Kaposi's sarcoma as well as primary effusion lymphoma and multicentric Castleman's disease. Therefore, vIL-6 is a promising target for novel therapies directed against HHV-8-associated diseases. By phage display screening of human synthetic antibody libraries, we have selected a specific recombinant antibody, called monoclonal anti-vIL-6 (MAV), binding to vIL-6. The epitope recognized by MAV was localized on the top of the D helix of the vIL-6 protein, which is a part of receptor binding site III. Consequently, MAV specifically inhibits vIL-6-mediated growth of the primary effusion lymphoma-derived cell line BCBL-1 and blocks STAT3 phosphorylation in the human hepatoma cell line HepG2. Since it was previously found that vIL-6 can also induce signals from within the cell, presumably within the endoplasmic reticulum, we fused the recombinant antibody MAV with the endoplasmic retention sequence KDEL (MAV-KDEL). As a result, COS-7 cells expressing MAV-KDEL and synthesizing vIL-6 ceased to secrete the cytokine. Moreover...

Expression of a virus-derived cytokine, KSHV vIL-6, in HIV-seronegative Castleman's disease.

Parravinci, C.; Corbellino, M.; Paulli, M.; Magrini, U.; Lazzarino, M.; Moore, P. S.; Chang, Y.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /12/1997 EN
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Castleman's disease is a rare B cell lymphoproliferative disorder related to excess interleukin-6 (IL-6)-like activity. Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8), which encodes a functional cytokine (vIL-6), has been found in some patients with Castleman's disease. Lymph nodes from 14 HIV-seronegative Castleman's disease patients were compared to hyperplastic lymph nodes from 25 HIV-seronegative patients as well as Kaposi's sarcoma lesions from 48 patients for KSHV infection and vIL-6, human IL-6, and Epstein-Barr virus EBER expression. While all Kaposi's sarcoma tissues examined were polymerase chain reaction-positive and all control lymph nodes were polymerase chain reaction-negative for KSHV, none had detectable vIL-6 expression. Six of 14 (43%) Castleman's tissues were positive for KSHV by polymerase chain reaction and all 6 had evidence of vIL-6 expression by immunohistochemistry. vIL-6-positive Castleman's disease patients generally had the multicentric plasma cell variant form of the disease and had a rapidly fatal clinical course frequently associated with autoimmune hemolytic anemia and gammopathy. In contrast, 7 (88%) of the 8 vIL-6-negative Castleman's disease patients had localized disease and have remained disease-free after therapy. KSHV vIL-6 expression appears to be limited to hematopoietic cells and is not present in Kaposi's sarcoma spindle cells. These data suggest that Castleman's disease is a syndrome of multiple etiologies involving aberrant IL-6 activity from either endogenous or viral sources.

Marek's Disease Virus-Encoded vIL-8 Gene Is Involved in Early Cytolytic Infection but Dispensable for Establishment of Latency

Cui, Xiaoping; Lee, Lucy F.; Reed, Willie M.; Kung, Hsing-Jien; Reddy, Sanjay M.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /05/2004 EN
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Marek's disease, a lymphoproliferative disease of chickens, is caused by an alphaherpesvirus, Marek's disease virus (MDV). This virus encodes a virokine, vIL-8, with general homology to cellular CXC chemokines such as interleukin-8 (IL-8) and Gro-α. To study the function of vIL-8 gene, we deleted both copies of vIL-8 residing in the terminal repeat long and internal repeat long region of the viral genome and generated a mutant virus with vIL-8 deleted, rMd5/ΔvIL-8. Growth kinetics study showed that vIL-8 gene is dispensable for virus replication in cell culture. In vivo, the vIL-8 gene is involved in early cytolytic infections in lymphoid organs, as evidenced by limited viral antigen expression of rMd5/ΔvIL-8. However, the rMd5/ΔvIL-8 virus is unimpaired in virus replication in the feather follicle epithelium. vIL-8 does not appear to be important for establishment of latency, since rMd5/ΔvIL-8 and the wild-type virus have similar viremia titers at 14 days postinfection, a period when the virus titer comes primarily from reactivated latent genomes. Nevertheless, because of the impaired cytolytic infections, the overall transformation efficiency of the virus with vIL-8 deleted is much lower, as reflected by the reduced number of transformed cells at 5 weeks postinoculation and the presence of fewer gross tumors. Importantly...

Kaposi's Sarcoma-Associated Herpesvirus (KSHV) vIL-6 Promotes Cell Proliferation and Migration by Upregulating DNMT1 via STAT3 Activation

Wu, Jing; Xu, Yuqiao; Mo, Dongping; Huang, Peijun; Sun, Ruihong; Huang, Lei; Pan, Shiyang; Xu, Jian
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 27/03/2014 EN
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Kaposi's sarcoma-associated herpesvirus (KSHV) is etiologically associated with Kaposi's sarcoma (KS), the most common AIDS-related malignancy. KSHV vIL-6 promotes KS development, but the exact mechanisms remain unclear. Here, we reported that KSHV vIL-6 enhanced the expression of DNA methyltransferase 1 (DNMT1) in endothelial cells,increased the global genomic DNA methylation, and promoted cell proliferation and migration. And this effect could be blocked by the DNA methyltransferase inhibitor, 5-azadeoxycytidine. We also showed that vIL-6 induced up-regulation of DNMT1 was dependent on STAT3 activation. Therefore, the present study suggests that vIL-6 plays a role in KS tumorigenesis partly by activating DNMT1 and inducing aberrant DNA methylation, and it might be a potential target for KS therapy.

Territoris sense model

Solà-Morales, Manuel de
Fonte: Universidade Autônoma de Barcelona Publicador: Universidade Autônoma de Barcelona
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em //1997 CAT
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La visió de les perifèries com a àmbits sense identitat espacial està relacionada amb l’absència de l’activitat i la construcció. Aquesta presència del buit és, però, la seva condició projectual més important. Les interpretacions d’aquests territoris han anat des del to denunciatori, unit a la crítica del creixement residencial especulatiu, la «perifèria vil», fins a la justificació esteticista del desordre territorial i l’empirisme perifèric, a partir de l’estima pels espais buits i descompostos, la «perifèria esplèndida». Sovint les aproximacions figuratives i les representacions del cinema i la fotografia han substituït un arsenal taxonòmic que manca encara, amb el qual definir les perifèries sense recórrer a la metàfora, l’analogia o la idea de degradació de la ciutat central. Les perifèries, però, apareixen com a espais actius del possible projecte contemporani de metròpoli. En aquest context, nous elements com la distància, argument compositiu de la forma urbana perifèrica, i el seu valor, que canvia en créixer l’escala del territori urbà considerat, mostren el protagonisme projectual de la separació vers la continuïtat unificadora de la ciutat compacta.; La visión de las periferias como ámbitos sin identidad espacial está relacionada con la ausencia de la actividad y la construcción. Esta presencia del vacío es su condición proyectual más importante. Las interpretaciones de estos territorios han ido desde el tono denunciatorio...

Grandes contenedores de cerámica -dolia- en la villa romana del cortijo del canal (Albolote, Granada)

Maeso Taviro, Carlos; Martinez-Sevilla, Francisco
Fonte: Universidade Autônoma de Barcelona Publicador: Universidade Autônoma de Barcelona
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em //2011 SPA
Relevância na Pesquisa
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Jornadas de Jóvenes en Investigación Arqueológica, JIA (3as : 5-7 de mayo 2010 : Universitat Autònoma de Barcelona). Sesión 8. El producto cerámico.; Este artículo se ofrecen los resultados obtenidos en las excavaciones realizadas en la villa romana del Cortijo del Canal (vega de Granada), y se analizan los elementos relacionados con la producción de aceite a través de la cerámica destinada para su almacenamiento, los dolia, realizando una ordenación tipológica y funcional de dicho contenedor.; This article presents de results obtained in the excavations at the roman villa Cortijo del Canal (Vega of Granada), and anlyces the elements related to the production of oil through the ceramic intended for storage, the dolia, by a typological an functional management of the container.; Aquest article ofereix els resultats obtinguts en les excavacions realitzades en la vil•la romana del Cortijo del Canal (Vega de Granada), i s’analitzen els elements relacionats amb la producció d’oli a traves de la ceràmica destinada per al seu emmagatzematge, les dolia, realitzant una ordenació tipològica i funcional d’aquest tipus de contenidor.