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Mechanical caracterisation of polyester and sunflower natural fiber composite

Rocha, João; Queijo, Luís
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
POR
Relevância na Pesquisa
36.1%
The use of natural fibers as reinforcement in polymer matrix composites is gaining popularity in the development of renewable products. Although glass and other synthetic fiber-reinforced plastics possess high specific strength, their fields of application are very limited because of their inherent higher cost of production. This work deals with the characteristics of natural fiber composites that can offer several advantages, like low cost, weight savings and relatively good mechanical properties. It focuses on the effects of sunflower fibers as reinforcement agents in composites. Mechanical testing of Sunflower natural fibers composite was performed to qualify and quantify the reinforcement properties. At same time, Glass fiber composite sample tests have been produced and characterized and both results have been compared. From results comparison we can affirm that the produced polymeric sunflower fiber composite has adequate mechanical properties for lots of industrial applications even, as expected, these are lower than glass fiber composites. Using this natural fiber content this product become a more ecological substitute and environment friendly that the traditional polymeric glass fiber composites. For other way, once stems from sunflower plants are wastes that traditionally are left in the field...

Mechanical caracterisation of polyester and sunflower natural fiber composite

Rocha, João; Queijo, Luís
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Artigo de Revista Científica
POR
Relevância na Pesquisa
36.1%
The use of natural fibers as reinforcement in polymer matrix composites is gaining popularity in the development of renewable products. Although glass and other synthetic fiber-reinforced plastics possess high specific strength, their fields of application are very limited because of their inherent higher cost of production. This work deals with the characteristics of natural fiber composites that can offer several advantages, like low cost, weight savings and relatively good mechanical properties. It focuses on the effects of sunflower fibers as reinforcement agents in composites. Mechanical testing of Sunflower natural fibers composite was performed to qualify and quantify the reinforcement properties. At same time, Glass fiber composite sample tests have been produced and characterized and both results have been compared. From results comparison we can affirm that the produced polymeric sunflower fiber composite has adequate mechanical properties for lots of industrial applications even, as expected, these are lower than glass fiber composites. Using this natural fiber content this product become a more ecological substitute and environment friendly that the traditional polymeric glass fiber composites. For other way, once stems from sunflower plants are wastes that traditionally are left in the field...

Efeito da inclusão de óleo de girassol, selênio e vitamina E na dieta de vacas lactantes sobre a qualidade e aceitabilidade do leite; Effect of sunflower oil, selenium and vitamin E inclusion in the diet of lactating cows on the quality and acceptability of milk

D'Abreu, Léa Furlan
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 27/02/2015 PT
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O objetivo do presente trabalho foi avaliar o efeito da suplementação com óleo de girassol, selênio e vitamina E em vacas lactantes, com base no perfil de ácidos graxos e estabilidade oxidativa da gordura do leite, além da aceitabilidade do produto pelos consumidores. Para tal, 32 vacas da raça Jersey em início de lactação foram distribuídas aleatoriamente entre os seguintes tratamentos dietéticos: C) controle; O) inclusão de 4% de óleo de girassol (% na MS); SE) inclusão de 3.000 UI de vitamina E/d + 3,5 mg de selênio/kg de MS; OSE) inclusão de 4% de óleo de girassol + 3.000 UI de vitamina E/d + 3,5 mg de selênio/kg de MS. Foram realizadas análises do perfil de ácidos graxos, TBARS e índice de peróxidos. Para caracterização sensorial do produto final, foram empregados os testes de aceitabilidade e diferença do controle. Os tratamentos dietéticos não influenciaram o desempenho produtivo e a composição do leite (P>0,05). Os animais suplementados com óleo de girassol produziram leite com elevado teor de ácido vacênico (P<0,0001) e ácido esteárico (P<0,05). O leite dos animais que receberam óleo de girassol apresentou menor concentração de C10:0, C12:0 e C14:0 (P<0,05). A inclusão dos antioxidantes na dieta não conseguiu retardar a oxidação do leite (P>0...

Fresh and matured lamb meat quality fed with sunflower seeds and vitamin E

Sales, Ronaldo Oliveira; Silva Sobrinho, Américo Garcia da; Zeola, Nivea Maria Brancacci Lopes; Lima, Natália Ludmila Lins; Manzi, Gabriela Milani; Almeida, Fabiana Alves de; Endo, Viviane
Fonte: Universidade Federal de Santa Maria (UFSM) Publicador: Universidade Federal de Santa Maria (UFSM)
Tipo: Artigo de Revista Científica Formato: 151-157
ENG
Relevância na Pesquisa
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O objetivo deste estudo foi avaliar a qualidade da carne de cordeiros Ile de France alimentados com dietas contendo grãos de girassol e vitamina E. Trinta e dois cordeiros pesando inicialmente 15kg foram alojados em baias individuais e abatidos ao atingir 32kg de peso corporal. Os tratamentos consistiram de quatro dietas experimentais: D1) cana-de-açúcar + concentrado sem grãos de girassol; D2) cana-de-açúcar + concentrado com grãos de girassol; D3) cana-de-açúcar + concentrado grãos de girassol, mais 1000mg de vitamina E kg-1 de matéria seca (MS) da dieta; e D4) cana-de-açúcar + concentrado com grãos de girassol e 1000mg de vitamina E dieta kg-1 de MS. Os parâmetros temperatura, pH, cor, perda de peso ao cozimento, força de cisalhamento e capacidade de retenção de água foram determinados nos tempos de maturação de 0, 7 e 14 dias no músculo Longissimus dorsi. As características qualitativas da carne não foram afetadas pelas dietas contendo grãos de girassol combinado com vitamina E, no entanto, pH 24 horas foi afetado (P<0,05) pela dieta contendo grãos de girassol com vitamina E. A perda de peso por cozimento não foi influenciada (P>0,05) pelos tempos de dieta ou de maturação, por outro lado, a força de cisalhamento e capacidade de retenção de água foi significativamente afetada (P<0...

Avaliação do desenvolvimento de girassol por meio de analise de proteinas e metaloproteinas; Evaluation of sunflower development based on protein metalloprotein analysis

Jerusa Simone Garcia
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 15/09/2006 PT
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Este trabalho de Tese apresenta os resultados referentes ao crescimento e ao desenvolvimento da cultura de girassol (Helianthus annuus L.) submetida a 7 tratamentos e as suas inter-relações com as análises de proteínas e metaloproteínas. Em quatro tratamentos, foram adicionadas soluções de íons metálicos (Cd, Cu, Pb e Zn) ao substrato. Em outros dois tratamentos, usados como testemunhos, foi empregada somente água desionizada para a irrigação das plantas. No último tratamento foi acrescentada uma solução denominada mista, contendo simultaneamente os 4 contaminantes. Nos tratamentos foi avaliada a altura das plantas, a biomassa produzida e a distribuição e translocação de nutrientes do substrato para as partes do girassol. As plantas submetidas à contaminação com a solução mista foram as mais afetadas em termos fisiológicos. Também foi investigada a influência das espécies metálicas na composição protéica. Para isso, foi feita a extração das proteínas presentes no sistema foliar, caulinar e radicular dos girassóis, sendo, em seguida, determinada a concentração das proteínas totais. As proteínas das folhas foram ainda separadas utilizando a eletroforese do tipo SDS-PAGE. Este processo possibilitou identificar...

Equilíbrio líquido-líquido em sistemas-modelos formados por óleo de semente de girassol + aldeídos + etanol anidro a 25 °C sob pressão atmosférica; Liquid-liquid equilibria for the model systems composed by sunflower seed oil + aldehydes + anhydrous ethanol at 25 °C under atmospheric pressure

Perci Odilon Bonetti Homrich
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 06/03/2015 PT
Relevância na Pesquisa
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O girassol é a oleaginosa que se apresenta em quarto lugar em relação à produtividade agrícola destinada à obtenção de óleos e possui vantagens por apresentar uma grande quantidade de ácido linoléico (ácido graxo polinsaturado) e compostos nutracêuticos, trazendo benefícios à saúde humana. O óleo de semente de girassol deve passar pelo processo de refino químico para remover a acidez e compostos minoritários para que se torne comestível. O processo de refino químico consiste, basicamente, de quatro etapas: degomagem, desacidificação, branqueamento e desodorização. A etapa de desodorização remove compostos odoríferos (principalmente aldeídos) formados pela oxidação do óleo, além de contaminantes, como pesticidas, por esgotamento com vapor de arraste sob altas temperaturas (até 265 °C) e alto vácuo. Essas condições drásticas de processamento causam a degradação e a volatilização de compostos nutracêuticos (principalmente antioxidantes), além de reações de oxidação e isomerização cis-trans de ácidos graxos insaturados. Neste contexto, este trabalho investigou a possibilidade de pré-tratar o óleo de girassol com solvente, removendo os odores do óleo bruto por extração líquido-líquido...

Integration of sunflower (Helianthus annuus) residues with a pre-plant herbicide enhances weed suppression in broad bean (Vicia faba)

Alsaadawi,I.S; Khaliq,A; Al-Temimi,A.A; Matloob,A
Fonte: Sociedade Brasileira da Ciência das Plantas Daninhas Publicador: Sociedade Brasileira da Ciência das Plantas Daninhas
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 EN
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Field trial was conducted with the aim of utilizing allelopathic crop residues to reduce the use of synthetic herbicides in broad bean (Vicia faba) fields. Sunflower residue at 600 and 1,400 g m-2 and Treflan (trifluralin) at 50, 75 and 100% of recommended dose were incorporated into the soil alone or in combination with each other. Untreated plots were maintained as a control. Herbicide application in plots amended with sunflower residue had the least total weed count and biomass, which was even better than herbicide used alone. Integration of recommended dose of Treflan with sunflower residue at 1,400 g m-² produced maximum (987.5 g m-2) aboveground biomass of broad bean, which was 74 and 36% higher than control and recommended herbicide dose applied alone, respectively. Combination of herbicide and sunflower residue appeared to better enhance pod number and yield per unit area than herbicide alone. Application of 50% dose of Treflan in plots amended with sunflower residue resulted in similar yield advantage as was noticed with 100% herbicide dose. Chromatographic analysis of residue-infested field soil indicated the presence of several phytotoxic compounds of phenolic nature. Periodic data revealed that maximum suppression in weed density and dry weight synchronized with peak values of phytotoxins observed 4 weeks after incorporation of sunflower residues. Integration of sunflower residues with lower herbicide rates can produce effective weed suppression without compromising yield as a feasible and environmentally sound approach in broad bean fields.

Fresh and matured lamb meat quality fed with sunflower seeds and vitamin E

Sales,Ronaldo Oliveira; Silva Sobrinho,Américo Garcia da; Zeola,Nivea Maria Brancacci Lopes; Lima,Natália Ludmila Lins; Manzi,Gabriela Milani; Almeida,Fabiana Alves de; Endo,Viviane
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2013 EN
Relevância na Pesquisa
36.16%
The objective of the study is to evaluate meat quality from Ile de France lamb fed diets supplemented with sunflower seeds and vitamin E. Thirty-two lamb weighing initially 15kg were housed in individual stalls and slaughtered when body weight reached 32kg. The treatments consisted of four experimental diets: D1) sugarcane + concentrate without sunflower seeds; D2) sugarcane + concentrate with sunflower seeds; D3) sugarcane + concentrate without sunflower seeds plus 1000mg vitamin E kg-1 diet dry matter (DM); and D4) sugarcane + concentrate with sunflower seeds and 1000mg vitamin E kg-1 diet DM. The parameters temperature, pH, color, cooking loss, shear force and water holding capacity were determined at maturation times of 0, 7 and 14 days on the Longissimus dorsi muscle. Qualitative characteristics of the meat were not affected by the sunflower seed combined with vitamin E diet; however, pH 24 hours was affected (P<0.05) by the sunflower seed combined with vitamin E diet. Cooking loss was not significantly influenced (P>0.05) by diet or maturation times. On the other hand, shear force and water holding capacity were significantly affected (P<0.05) by the treatments. The use of vitamin E resulted in lamb meat with higher water holding capacity. Maturation time influenced the parameters pH...

Chemical changes in sunflower silage associated with different additives

Goes,Rafael Henrique de Tonissi e Buschinelli de; Miyagi,Eliane Sayuri; Oliveira,Euclides Reuter de; Brabes,Kelly Cristina da Silva; Patussi,Rosielen Augusto; Dambrós,Carlos Eduardo
Fonte: Editora da Universidade Estadual de Maringá - EDUEM Publicador: Editora da Universidade Estadual de Maringá - EDUEM
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2013 EN
Relevância na Pesquisa
36.18%
Thirty-six experimental silos arranged in a completely randomized 4 x 3 factorial design were provided to evaluate the chemical changes of sunflower silage treated with soybean hulls, sunflower crushed and urea at 14, 21 and 28 days of ensilage. The additives were based on 5% natural matter, whereas control consisted of silage with 100% sunflower plant. OM, NDIP, and MM had average rates 911.2; 86.6 and 92.9 g kg-1 of dry matter respectively. The addition of soybean hulls and sunflower crushed increased DM rates after 28 and 21 days. Urea increased nitrogen fractions and the addition of soybean hulls increased total carbohydrate content of silage by 5.1%, whereas the addition of sunflower crushed decreased the same by 2.18%. NDF, ADF and hemicellulose average rates were 625.4, 460.3 and 165.2 g kg-1 of DM. The addition of soybean hulls and sunflower crushed reduced the in vitro dry matter digestibility by 8.3 and 5.97%. The addition of 5% sunflower crushed and soybean hulls improved the nutritional value of sunflower silage and the addition of urea improved the protein rates.

Sunflower tolerance to aluminum.

CASTRO, C. de; MORAES, L. A. C.; OLIVEIRA JUNIOR, A. de; LEITE, R. M. V. B. C.; OLIVEIRA, F. A. de; RODAK, B. W.
Fonte: In: INTERNATIONAL SUNFLOWER CONFERENCE, 18., 2012, Mar del Plata & Balcarce. Proceedings... [Mar del Plata]: ISA: ASAGIR, 2012. Publicador: In: INTERNATIONAL SUNFLOWER CONFERENCE, 18., 2012, Mar del Plata & Balcarce. Proceedings... [Mar del Plata]: ISA: ASAGIR, 2012.
Tipo: Artigo em anais de congresso (ALICE) Formato: 1 CD-ROM.; 4 p.
EN
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36.13%
Sunflower is very sensitive to soil acidity and, in general, cannot tolerate Al saturation greater than 5%. Under these conditions, root growth is drastically affected reducing the ability of plants to explore larger soil volume, which consequently may block water and nutrient absorption. The aim of this study was to evaluate the tolerance of sunflower genotypes to four aluminum concentrations. An experiment was carried out at the Seed Laboratory of Embrapa Soja. Seeds of ten sunflower genotypes (Pioneer 6510, CF 101, Embrapa 122, BRS 323, BRS 322, V 70004, SYN 045, BRS G 32, HLA 211 CL and Catissol) were put directly on germination paper moistened with four doses of aluminum (0, 0.4, 0.8 and 1.2 mg dm-3 Al), using Al2(SO4)3.18H2O. Ten seeds were used for each treatment, with three replications. Rolls were placed in a growth chamber, in the dark and at a temperature of 25 ° C. Dry weight of roots was evaluated at seven days after. Dry weight of roots of sunflower genotypes was significantly affected by levels of aluminum in solution. Genotypes BRS 322, BRS 323 and BRS G 32 produced higher dry weight of roots, which was statistically superior to genotypes Catissol, Embrapa 122, SYN 045 and HLA 211 CL. The method is able to separate sunflower genotypes by development of roots under different concentrations of aluminum.; 2012

Avaliação do potencial fitorremediador da mamona (Ricinus communis L.) e girassol (Helianthus annuus L.) quanto à remoção de chumbo e tolueno em efluentes sintéticos.; Avaliação do potencial fitorremediador da mamona (Ricinus communis L.) e girassol (Helianthus annuus L.) quanto à remoção de chumbo e tolueno em efluentes sintéticos.; Assessment of potential for phytoremediation of castor bean (Ricinus communis L.) and sunflower (Helianthus annuus L.) as the removal of lead and toluene in synthetic wastewater; Assessment of potential for phytoremediation of castor bean (Ricinus communis L.) and sunflower (Helianthus annuus L.) as the removal of lead and toluene in synthetic wastewater

Lima, Anita Maria de
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Engenharia Química; Pesquisa e Desenvolvimento de Tecnologias Regionais Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Engenharia Química; Pesquisa e Desenvolvimento de Tecnologias Regionais
Tipo: Tese de Doutorado Formato: application/pdf
POR
Relevância na Pesquisa
36.15%
The development of research that aim to reduce or even eliminate the environmental impacts provided by anthropogenic actions. One of these main action is the discard of industrial waste in the biotic compartments such as soil, water and air, gained more space in academic settings and in private. A technique of phytoremediation involving the use of plants (trees, shrubs, creepers and aquatic) and their associated microorganisms in order to remove, degrade or isolate toxic substances to the environment. This study aimed to evaluate the potential for phytoremediation of castor bean (Ricinus communis L.) and sunflower (Helianthus annuus L.), wild crops suitable region of Rio Grande do Norte, to reduce concentrations of lead and toluene present in synthetic wastewater that simulate the characteristics of treated water production originated in the petrochemical Guamaré. The experiment was accomplished in randomized blocks in four replicates. Seeds of BRS Energy for the development of seedlings of castor beans and sunflower for Catissol 01, both provided by EMPARN (Empresa de Pesquisa Agropecuária do Rio Grande do Norte) were used. Lead concentrations tested were 250, 500 and 1000 mg/L called T2, T3 and T4, respectively, for toluene the concentrations used were 125...

Isolado proteico de girassol obtenção e propriedades; Isolated protein sunflower production and properties

Cristina Garibay Bagnis
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 18/07/1984 PT
Relevância na Pesquisa
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Neste trabalho foram estudadas as características físicas e químicas de oito variedades de sementes de girassol de maior importância no Brasil. Dentre todas as variedades estudadas, o novo cultivar IAC-Anhandy foi o que apresentou os maiores teores de óleo e amêndoa. A concentração de proteína nas sementes das diversas variedades variou entre 15,38 e 25,28%. As proteínas de girassol mostraram deficiência em lisina (3,5g/l00g de proteína) e um alto conteúdo de aminoácidos sulfurados (5,2g/l00g de proteína) em relação ao padrão da FAO, 1973. Verificou-se que o óleo das oito variedades de girassol conteve urna elevada porcentagem de ácidos graxos insaturados (86,3 a 91,1%), principalmente ácidos linoleico (37,4 a 69,0%) e oleico (19,9a 53,7%). O conteúdo de ácido clorogênico na farinha desengordurada das variedades de girassol variou entre 3,60' e 5,51% na base seca. Também foram determinadas as condições ótimas para a extração das proteínas d~.girassol, a partir da farinha desengordurada obtida de sementes do cultivar IAC-Anhandy. O maior rendimento na extração das proteínas,94%,foi obtido utilizando-se as seguintes condições: relação farinha:água, 1:10 (p/v); pH, 10,0; temperatura, 309C; tempo de agitação...

Socio-environmental impacts of sunflower production as feedstock for biodiesel in southern Minas Gerais/BR.

RAMOS, N. P.; BUSCHINELLI, C. C. A.; FONTES, L. P.; VIEIRA, H. B.; CASTRO, C.; LEITE, R. M. V. B. C.
Fonte: In: INTERNATIONAL SUNFLOWER CONFERENCE, 18., 2012, Mar del Plata & Balcarce. Proceedings... [Mar del Plata]: ISA: ASAGIR, 2012. Publicador: In: INTERNATIONAL SUNFLOWER CONFERENCE, 18., 2012, Mar del Plata & Balcarce. Proceedings... [Mar del Plata]: ISA: ASAGIR, 2012.
Tipo: Artigo em anais de congresso (ALICE) Formato: 1 CD-ROM.; 5 p.
EN
Relevância na Pesquisa
36.18%
The increase in global demand for renewable energy has encouraged, both directly and indirectly, the production of oilseeds, including sunflower, as feedstocks for biodiesel. In this scenario, Brazil stands out for its excellent agronomic and climatic conditions for growing these crops throughout its territory. However, there is still little information about the sustainability of the biodiesel production chain, depending on the source of raw materials and inputs, the rural context and the region where it is produced. Faced with these questions, this study aimed at evaluating the social and environmental impacts, both along the production chain of biodiesel obtained from sunflower, and at the rural establishment level, in the reference context of southern Minas Gerais state, Southeast Brazil. The methodology involved application of two impact assessment tools: a) the ?Base System for Eco-certification of rural activities' (Eco-cert.Rural)? comprised of 24 criteria and 125 indicators for performance evaluation at the value-chain level (gathering 19 experts with knowledge of different links in the biodiesel chain within the studied area) and b) "System of Environmental Impact Assessment of Rural Activities" (APOIA-NovoRural), comprised of 62 sustainability indicators applied to the rural establishment scale (through a detailed field survey / interview involving a reference sunflower producer). The study was conducted in November 2009 and information from the interviews were inserted directly into the weighting matrices of the systems...

Severe phosphorus stress affects sunflower and maize but not soybean root to shoot allometry.

Rubio, Gerardo; Gutiérrez Boem, Flavio Hernán; Fernández, Mariana Cecilia
Fonte: Amer Soc Agronomy Publicador: Amer Soc Agronomy
Tipo: info:eu-repo/semantics/article; info:ar-repo/semantics/artículo; info:eu-repo/semantics/publishedVersion Formato: application/pdf
ENG
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The predictions of two models on biomass allocation were compared on P-stressed and non-stressed crop plants. Allometric coefficients were calculated from paired measurements of root and shoot biomass obtained from field and greenhouse experiments with soybean [Glycine max (L.) Merr.], sunflower (Helianthus annuus L.), and maize (Zea mays L.) plants. Soybean consistently followed the allometric model, with the allocation pattern governed by the plant size (common slope K of 0.96 and 0.82 in the field and greenhouse, respectively). Sunflower and maize showed allometric trajectories in the field but optimal partitioning trajectories in the greenhouse. Field data for sunflower and maize adjusted to a unique line (K = 0.92 and 1.05, respectively) indicating that the biomass allocation is explained by allometric trajectories irrespective of the P level. In contrast, greenhouse data adjusted to two parallel lines (i.e., different elevation coefficient but similar slope: 0.91 for sunflower, 0.96 for maize). Only under severe P stress sunflower and maize plants modified their allocation pattern. Since the severity of the P stress needed to induce a shift in the allocation pattern would be large, we conclude that the three species follow unique root to shoot allometric trajectories under P levels usually found in the field. Most studies analyze the slope of the allometric relationships irrespective of the intercept. Here...

Mapeo por asociación en girasol: diversidad nucleotídica, desequilibrio de ligamiento e identificación de genes involucrados en la resistencia a la podredumbre húmeda del capítulo; Association mapping in sunflower: nucleotide diversity, linkage disequilibrium and identification of genes involved in sclerotinia head rot resistance

Fusari, Corina Mariana
Fonte: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires Publicador: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Tipo: info:eu-repo/semantics/doctoralThesis; tesis doctoral; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2010 SPA
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El mapeo por asociación es una herramienta poderosa que permite la identificación de loci cuya contribución explica parte de la variación fenotípica observada. En general, los marcadores utilizados en estos estudios son los Polimorfismos de Nucleótido Simple (SNPs, Single Nucleotide Polymorphisms) y los indels (eventos de inserción, insertion/deletion). Los objetivos de este trabajo comprendieron el estudio de la diversidad nucleotídica y el alcance del desequilibrio de ligamiento (DL) en girasol cultivado, y el diseño de una estrategia de mapeo por asociación para la resistencia a la Podredumbre Húmeda del Capítulo (PHC), causada por Sclerotinia sclerotiorum. La frecuencia de SNPs (1/61) y la diversidad nucleotídica (θ=0,0067) se determinaron sobre un panel de 31 genes y 19 líneas endocriadas de girasol. La frecuencia de SNPs en conjunto con un alcance del DL hasta 100 kb (r2~0,1) permitieron diseñar un estudio de asociación a través de la estrategia de genotipificación de genes candidatos. Un total de 30 genes candidatos fueron seleccionados a partir de: (1) el análisis de los perfiles transcripcionales de un genotipo de Brassica napus resistente a S. sclerotiorum, (2) una colección de transcriptos de expresión diferencial de una línea de girasol moderadamente resistente desafiada con S. sclerotiorum y (3) genes descriptos en literatura como partícipes en los procesos de defensa frente a PHC. La elección...

Identificación y caracterización funcional de genes candidatos asociados a la senescencia foliar en girasol basado en perfiles transcripcionales y metabólicos; Identification and functional characterization of candidate genes associated with leaf senescence in sunflower based on transcriptional and metabolic profiles

Moschen, Sebastián
Fonte: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires Publicador: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Tipo: info:eu-repo/semantics/doctoralThesis; tesis doctoral; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2014 SPA
Relevância na Pesquisa
36.21%
El proceso de senescencia en plantas es un mecanismo complejo controlado por múltiples variables genéticas y ambientales que condicionan el rendimiento de los cultivos. En el caso del girasol, el segundo cultivo oleaginoso en importancia económica para nuestro país, se trata de un proceso con impacto económico que interviene en la brecha existente entre el rendimiento potencial y el rendimiento real observado, por la mayor o menor oportunidad de las plantas para mantener el sistema fotosintético activo durante periodos prolongados. Los parámetros visuales resultan tardíos para evaluar el desencadenamiento y posterior tasa de evolución de la senescencia foliar. La clorosis, la variación en el contenido de clorofila así como también la necrosis de las hojas, son detectables mucho tiempo después que la señal iniciadora de la senescencia ha sido activada. Este trabajo tuvo como objetivo general el estudio del proceso de senescencia en girasol a través de distintos niveles de organización: ecofisiológico, metabolómico, transcriptómico, culminando con la integración de los diversos enfoques ómicos mediante una aproximación de biología de sistemas, con el objetivo final de identificar potenciales biomarcadores asociados al proceso de senescencia en girasol. Se condujeron distintos ensayos que fueron realizados tanto a campo...

Nuevas estrategias para la transformación y expresión de genes de interés en girasol; New strategies for the transformation and expression of genes of interest in sunflower

Radonic, Laura Mabel
Fonte: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires Publicador: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Tipo: info:eu-repo/semantics/doctoralThesis; tesis doctoral; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2010 SPA
Relevância na Pesquisa
36.13%
El girasol es una especie de la familia Asteraceae (Compositae) de gran importancia económica, considerada hasta hace una década recalcitrante al cultivo in vitro y la transformación genética. En un trabajo previo (Radonic, 2005) se logró establecer el protocolo de selección por enraizamiento en kanamicina con una eficiencia del 0,7 %. En esta tesis se mejoró este protocolo utilizando una construcción portadora de los genes antifúngicos glucanasa y quitinasa, ambos bajo el control del promotor CaMV35S y el enhancer Ω del TMV, obteniéndose una eficiencia de 1,26 %, al aumentar gradualmente la concentración del antibiótico kanamicina en los sucesivos medios de regeneración. También se evaluó la especificidad y estabilidad de dos vectores conteniendo la construcción CaMV35S-secuencia codificante para la β-glucuronidasa (GUS)-interrumpida por un intrón. Las plantas T1 derivadas de los eventos de transformación presentaron un patrón de expresión de tipo no constitutivo, con expresión de GUS localizada exclusivamente en los tricomas de las nervaduras de la cara abaxial de las hojas, siendo necesario la utilización de lupa para su visualización. En las plantas T2 no se pudo detectar la presencia de los transgenes. El nivel bajo de expresión es similar al descripto en crisantemo y la inestabilidad génica obtenida con este mismo promotor fue también descripta en lechuga...

Sunflower oil having a high stearic acid content

Osorio, Jorge; Fernández-Martínez, José María; Mancha Perello, Manuel; Garcés Mancheño, Rafael
Fonte: Conselho Superior de Investigações Científicas Publicador: Conselho Superior de Investigações Científicas
Tipo: Patente Formato: 698371 bytes; application/pdf
ENG
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36.18%
Filing Date: 1995-01-31.-- Priority Data: ES (1994-06-24)P-(1994-01-31); The invention relates to a sunflower seed, comprising a sunflower oil having an increased stearic acid content as compared to wild type seeds, obtainable by treating parent seeds with a mutagenic agent during a period of time and in a concentration sufficient to induce one or more mutations in the genetic trait involved in stearic acid biosynthesis resulting in an increased production of stearic acid, germinating the treated seeds and culturing progeny plants therefrom, collecting and analyzing progeny seeds, selecting seeds that have acquired the desirable genetic trait and optionally repeating the cycle of germination, culturing and collection of seeds. Preferably the seeds comprise an oil having a stearic acid content of between 19.1 and 35 % by weight related to the total amount of fatty acids in the oil, and are obtainable by treating the parent seeds with an alkylating agent, such as ethyl methane sulfonate in water, or with sodium azide in water. The invention further relates to sunflower oil obtainable by extracting the sunflower seeds, to a method for preparing sunflower seeds having an increased stearic acid content as compared to wild type seeds, a method for preparing a sunflower oil having an increased stearic acid content sunflower plants produced from the seeds and the use of the sunflower oil in various products.; Peer reviewed

Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes

Talia,Paola; Greizerstein,Eduardo J.; Hopp,H. Esteban; Paniego,Norma; Poggio,Lidia; Heinz,Ruth A.
Fonte: Biocell Publicador: Biocell
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2011 EN
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36.13%
Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect C-PRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower.

Oleic conversion effect on the tocopherol and phytosterol contents in sunflower oil

Ayerdi Gotor,A; Berger,M; Labalette,F; Centis,S; Daydé,J; Calmon,A
Fonte: Phyton (Buenos Aires) Publicador: Phyton (Buenos Aires)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2014 EN
Relevância na Pesquisa
36.13%
In sunflower, conventional breeding is widely used for the modification of traits such as the fatty acid composition, disease resistance, and mainly for obtaining commercial oil with high oleic acid content. There is a growing interest on tocopherols and phytosterols present in sunflower, due to their human health benefits. This emphasizes the need of studies on breeding programs for these bioactive components. A hundred of isogenic pairs of classic and its oleic version of hybrids and parental lines were cultivated in different locations in France between 2003 and 2006. The results indicated that sunflower oil is rich in α-tocopherol and β-sitosterol. However, there was little correlation between traditional linoleic and oleic sunflower oils for the total tocopherol content, and no correlation for the total phytosterol content. Additionally, there was little or no effect of the oleic conversion for the tocopherol and phytosterol contents. Nevertheless, tocopherol content was significantly lower in the oleic sunflower than in the classic genotypes, but it was function of the year.