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Novel sequence types (STs) of Staphylococcus aureus isolates causing clinical and subclinical mastitis in flocks of sheep in the northeast of Brazil

ALMEIDA, Lara M. de; ALMEIDA, Mayra Zilta P. R. B. de; MENDONCA, Carla L. de; MAMIZUKA, Elsa M.
Fonte: CAMBRIDGE UNIV PRESS Publicador: CAMBRIDGE UNIV PRESS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
46.41%
Staphylococcus aureus is one of the most important infectious mastitis causative agents in small ruminants. In order to know the distribution of Staph. aureus strains associated with infectious mastitis in flocks of sheep in the northeast of Brazil and establish whether these clones are related to the strains distributed internationally, this study analysed the genetic diversity of Staph. aureus isolates from cases of clinical and subclinical mastitis in ewes by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In this research, 135 ewes with mastitis from 31 sheep flocks distributed in 15 districts were examined. Staph. aureus was isolated from sheep milk in 9 (29%) out of 31 herds located in 47% of the districts surveyed. MLST analysis allowed the identification of four STs (ST750, ST1728, ST1729 and ST1730). The last three with their respective novel alleles (g/p-220; pta-182 and yqil-180) were recently reported in the Staph. aureus MLST database (http://www.mlst.net). Each novel allele showed only a nucleotide different from those already described. The occurrence of CC133 (ST750 and ST1729) in this study is in agreement with other reports that only a few clones of Staph. aureus seem to be responsible for most cases of mastitis in dairy farms and that some of these clones may have broad geographic distribution. However...

Relationship between udder measurements and milk yield in Bergamasca ewes in Brazil

Emediato, R. M. S.; Siqueira, E. R.; Stradiotto, M. M.; Maesta, S. A.; Fernandes, S.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 232-235
ENG
Relevância na Pesquisa
46.31%
The present work aimed to evaluate udders of Bergamasca ewes and their correlation with milk yield in a mix system of milk yield. Seventy-seven ewes were fed with balanced concentrate starting 20 days before lambing until the end of the experiment. The lambs stayed with their mothers in pastures during the day and were separated at night. They returned to their mothers after the morning milking and were weaned at 45 days of age. Forty-eight hours after lambing, ewes were machine milked once daily at 7 am and the milk yield was recorded for a period of 60 days. Measurements of circumference, depth and width of the udder, and width and length of teats, at 30 and 60 days, were taken. A higher average daily yield of commercial milk was observed after lambs weaning (0.509 vs. 0.435 kg/ewe/day) than before. In the same way, the correlations between udder depth, circumference and width and milk yield were positive and significant only after weaning (0.74, 0.75 and 0.62, respectively). Udder measures had positive correlations with milk yield and can be used in programs of milk yield improvement. (c) 2007 Elsevier B.V. All rights reserved.

Detection of Toxoplasma gondii DNA in the milk of naturally infected ewes

Camossi, L. G.; Greca-Junior, H.; Correa, A. P. F. L.; Richini-Pereira, V. B.; Silva, R. C.; Da Silva, A. V.; Langoni, Helio
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 256-261
ENG
Relevância na Pesquisa
46.71%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 08/07876-8; Processo FAPESP: 08/05854-1; Toxoplasmosis is the major parasitic disease affecting sheep. It is important for veterinary medicine, animal science and public health since it causes reproductive and economic losses in the herd, as well as damaging human health due to consumption of contaminated meat and milk, which can facilitate zoonotic transmission. Detection of Toxoplasma gondii in ovine milk and lack of data in the literature describing differentiation between acute and chronic disease for this species stimulated the elaboration of the present research project. To achieve the aim of this study, the animals were allocated to two groups of 20 ewes each, of which group 1 was composed of animals with positive serology and group 2 with negative serology. Acute and chronic stages of the disease were differentiated by modified direct agglutination test (MAT), in which antigens were fixed with formalin (MAT-AF) and methanol (MAT-AM). The parasite was detected in milk by polymerase chain reaction (PCR), and the molecular identity of the amplified products was confirmed by sequencing. The serological results indicated that sheep had a chronic infection profile. T. gondii DNA was detected in seven milk samples from five seropositive sheep...

Aspectos de produção e etio-epidemiológicos referentes às vias de transmissão nas mastites ovinas

Bolsanello, Roberto Ximenes
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Dissertação de Mestrado Formato: 96 f.
POR
Relevância na Pesquisa
36.67%
Pós-graduação em Medicina Veterinária - FMVZ; A ovinocultura vem se expandindo no Brasil nos últimos anos, e com isso começa-se a observar também um maior interesse para a produção de leite dessa espécie. Mesmo em países cuja produção leiteira é uma tradição de muitos anos, há uma deficiência de novas tecnologias e de pesquisas ligadas à produção de leite ovino. O leite de ovelha é um produto caro, com nichos de mercados e com excelentes perspectivas de crescimento no Brasil. Este trabalho teve como finalidade estudar aspectos de etio-epidemiologia referentes às vias de transmissão de patógenos na mastite ovina, e os aspectos de produção leiteira em animais com ou sem mastite, durante toda lactação. O experimento foi realizado na área de Produção de Ovinos da Fazenda Edgárdia, da Faculdade de Medicina Veterinária e Zootecnia – UNESP – Campus de Botucatu. Foram utilizadas 37 ovelhas em lactação, da raça Bergamácia, submetidas a ordenha mecânica diariamente, com manejo idêntico. Os animais foram acompanhados durante toda a lactação (8 semanas). Avaliou-se o índice de mastite através do Califórnia Mastitis Test (CMT) e o leite dos animais positivos foi realizada cultura microbiológica e também se realizou a cultura microbiológica na água utilizada para higienização das tetas e equipamentos de ordenha; nos insufladores (teteiras); nas mãos dos ordenhadores; no óstio das tetas e na orofaringe dos respectivos cordeiros para correlacionar os achados microbiológicos encontrados nas diferentes situações...

Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk

Martins, Katheryne Benini; Faccioli-Martins, Patricia Yoshida; Riboli, Danilo Flávio Moraes; Pereira, Valéria Cataneli; Fernandes, Simone; Oliveira, Aline A.; Dantas, Ariane; Zafalon, Luiz Francisco; Cunha, Maria De Lourdes Ribeiro De Souza da
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: 535-543
ENG
Relevância na Pesquisa
56.46%
Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer...

Características físico-químicas e microbiológicas do leite de ovelha e atividade antagonista de sua microbiota lática; Physico-chemical and microbiological characteristics of sheep milk and antagonist activity of its lactic microbiota

Koch, Anna Carolina da Costa
Fonte: Universidade de Brasília Publicador: Universidade de Brasília
Tipo: Tese
POR
Relevância na Pesquisa
46.61%
Tese (doutorado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, 2014.; Texto parcialmente liberado pelo autor. Conteúdo restrito: capítulo 3.; A presente pesquisa avaliou a qualidade do leite de ovelhas criadas no Distrito Federal com o objetivo de determinar a sua qualidade e potencial tecnológico. Foram coletadas 126 amostras de leite cru diretamente da glândula mamária de ovelhas, das raças Santa Inês, Dorper e ovelhas leiteiras East Friesian e suas mestiças (EF x SI) em 15 propriedades rurais, que foram submetidas à análises das características físico-químicas (pH, acidez Dornic, densidade, crioscopia, gordura, sólidos não gordurosos, proteína e lactose), microbiológica por meio da enumeração de aeróbios mesófilos (AM), coliformes totais (CT) e Escherichia coli, Staphylococcus aureus e detecção de Listeria monocytogenes e Salmonella spp.. A avaliação da atividade antimicrobiana das amostras de leite foi realizada pela enumeração, identificação e caracterização da atividade antimicrobiana de BAL. No total 78 isolados foi testado quanto ao potencial antagonista frente a quatro cepas de L. monocytogenes, quatro cepas S. aureus e uma cepa de Lactobacillus sakei; em seguida...

Identification of peptides in traditional and probiotic sheep milk yoghurt with angiotensin I-converting enzyme (ACE)-inhibitory activity

Papadimitriou, Christos G.; Vafopoulou-Mastrojiannaki, Anna; Silva, Sofia Vieira; Gomes, Ana-Maria; Malcata, Francisco Xavier; Alichanidis, Efstathios
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /04/2007 ENG
Relevância na Pesquisa
66.4%
Two sets of traditional Greek sheep milk yoghurt were produced: the first one (YC) using normal yoghurt culture (Lactobacillus delbrueckii subsp. bulgaricus !10.13 and Streptococcus thermophilus !10.7) and the second (PR) with the same normal culture mixed with Lactobacillus paracasei subsp. paracasei DC412. YC and PR had similar physicochemical properties and proteolysis patterns throughout storage. Both products showed similar peptide profiles by RP-HPLC but quantitative differences were observed in respect to storage time. Single-strain cultures of the microorganisms used showed similar peptide profiles for both lactobacilli, yet L. delbrueckii subsp. bulgaricus was the most proteolytic of all three microorganisms. The peptide content and the ACE-inhibitory activity of the water-soluble extracts of yoghurts, YC and PR, increased throughout storage. Major peptides were identified from yoghurt PR and from the separate cultures of L. delbrueckii subsp. bulgaricus and L. paracasei subsp. paracasei. Most of these peptides were derived from b-casein. A peptide, b-CN f114-121, with well-established ACE-inhibitory and opiate-like activity was identified in yoghurt PR. Further identified peptides were regarded as potential ACE-inhibitors according to their sequence.

Isolation, enumeration, molecular identification and probiotic potential evaluation of lactic acid bacteria isolated from sheep milk

Acurcio,L.B.; Souza,M.R.; Nunes,A.C.; Oliveira,D.L.S.; Sandes,S.H.C.; Alvim,L.B.
Fonte: Universidade Federal de Minas Gerais, Escola de Veterinária Publicador: Universidade Federal de Minas Gerais, Escola de Veterinária
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 EN
Relevância na Pesquisa
66.51%
Lactic acid bacteria species were molecularly identified in milk from Lacaune, Santa Inês and crossbred sheep breeds and their in vitro probiotic potential was evaluated. The species identified were Enterococcus faecium (56.25%), E. durans (31.25%) and E. casseliflavus (12.5%). No other lactic acid bacteria species, such as lactobacilli, was identified. Most of the isolated enterococci were resistant to gastric pH (2.0) and to 0.3% oxgall. All tested enterococci were resistant to ceftazidime, oxacillin and streptomycin and sensible to clindamycin, erythromycin and penicillin. The resistance to ciprofloxacin, gentamicin, tetracycline and vancomycin varied among tested species. All tested enterococci strongly inhibited (P<0.05) Escherichia coli and Listeria monocytogenes, moderately inhibited E. faecalis and Staphylococcus aureus and did not inhibit Pseudomonas aeruginosa, Salmonella enterica var. Typhimurium and also one E. durans sample isolated from sheep milk. Four samples of E. faecium, one of E. durans and one of E. casseliflavus presented the best probiotic potential.

Feeding strategies to design the fatty acid profile of sheep milk and cheese

Nudda,Anna; Battacone,Gianni; Boaventura Neto,Oscar; Cannas,Antonello; Francesconi,Ana Helena Dias; Atzori,Alberto Stanislao; Pulina,Giuseppe
Fonte: Sociedade Brasileira de Zootecnia Publicador: Sociedade Brasileira de Zootecnia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2014 EN
Relevância na Pesquisa
66.68%
The majority of sheep milk produced in the world is transformed into cheese. Feeding is a major factor affecting the quality of sheep milk and, therefore, of sheep cheese. Because fat is the main compound of cheese, this review gives an update on the effects of feeding and nutrition on milk fat content and deeply discusses feeding strategies aimed at increasing the levels of healthy fatty acids (FA), such as conjugated linoleic acid and omega-3 FA, in milk and cheese in the human diet. In addition, the use of alternative feed resources such as by-products, aromatic plants, and phenolic compounds in the sheep diet and their effects on milk and cheese FA composition are also discussed. Among feeding strategies, grazing and the use of supplements rich in oils seem to be the best and the cheapest strategies to improve the nutritional value of the fatty acid profile in sheep cheese.

A quantitative study of enterotoxin production by sheep milk staphylococci.

Bautista, L; Gaya, P; Medina, M; Nuñez, M
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1988 EN
Relevância na Pesquisa
46.23%
Of 124 staphylococcal strains isolated from sheep milk, 78 produced enterotoxin A, B, C, or D when evaluated by an enzyme-linked immunosorbent assay. Enterotoxins A and D, elaborated by 44 and 43 strains, respectively, showed the highest incidence. Enterotoxin production by coagulase-negative strains (one Staphylococcus cohnii, three S. epidermidis, five S. haemolyticus, and four S. xylosus) was detected. Linear and logarithmic-logarithmic regressions of optical density on enterotoxin concentration yielded the best-fitting equations for enterotoxin quantitation. A significantly higher incidence of enterotoxin producers and significantly higher levels of enterotoxins produced were recorded for coagulase-positive, thermostable nuclease-positive, hemolysis-positive, or mannitol-positive strains. Mannitol utilization was the best test for discriminating between enterotoxigenic and nonenterotoxigenic staphylococci.

Staphylococcal food poisoning from sheep milk cheese.

Bone, F. J.; Bogie, D.; Morgan-Jones, S. C.
Fonte: Cambridge University Press Publicador: Cambridge University Press
Tipo: Artigo de Revista Científica
Publicado em /12/1989 EN
Relevância na Pesquisa
46.49%
Cheese made from sheep milk was implicated in food-poisoning incidents in December 1984 and January 1985. Bacteriological examination of batches of cheese failed to reveal a viable pathogen but enterotoxin A produced by Staphylococcus aureus was present. This was the first time that enterotoxin was detected in a food produced in the UK which was associated with poisoning and from which viable Staph. aureus could not be isolated. Subsequent detailed examination of milk, yoghurt and cheese from the same producer revealed that contamination with Staph. aureus was associated with post-infection carriage as well as clinical illness in ewes on the farm. Strains producing enterotoxon. A were still intermittently present in the bulk milk used for cheese production nearly 2 years afterwards, apparently in the absence of clinical illness in the sheep. The possible effects of heat treatment are discussed. Any changes in legislation should cover all non-human mammalian milk used for human consumption.

Dairy sheep production research at the University of Wisconsin-Madison, USA – a review

Thomas, David L; Berger, Yves M; McKusick, Brett C; Mikolayunas, Claire M
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 16/04/2014 EN
Relevância na Pesquisa
36.71%
Commercial milking of sheep is a new agricultural industry in the United States starting approximately 30 yr ago. The industry is still small, but it is growing. The majority of the sheep milk is used in the production of specialty cheeses. The United States is the major importer of sheep milk cheeses with 50 to 60% of annual world exports coming to the United States during the past 20 yr. Therefore, there is considerable growth potential for the industry in the United States. The only dairy sheep research flock in North America is located at the Spooner Agricultural Research Station of the University of Wisconsin-Madison. The research program started in 1993 and has been multifaceted; dealing with several areas important to commercial dairy sheep farmers. The East Friesian and Lacaune dairy breeds were compared and introduced to the industry through the research program. Both dairy breeds produced significantly more milk than traditional meat-wool breeds found in the U.S., but the two breeds differed in their production traits. East Friesian-cross ewes produced more lambs and slightly more milk than Lacaune-cross ewes whereas Lacaune-cross ewes produced milk with a higher percentage of fat and protein than East Friesian-cross ewes. Lactation physiology studies have shown that ewes with active corpora lutea have increased milk yields...

Prevalence, pathogenic capability, virulence genes, biofilm formation, and antibiotic resistance of Listeria in goat and sheep milk confirms need of hygienic milking conditions

Osman, Kamelia M; Zolnikov, Tara Rava; Samir, Ahmed; Orabi, Ahmed
Fonte: Maney Publishing Publicador: Maney Publishing
Tipo: Artigo de Revista Científica
Publicado em /01/2014 EN
Relevância na Pesquisa
46.75%
Goat and sheep milk is consumed by human populations throughout the world; as a result, it has been proposed as an alternative, nutrient-rich milk to feed infants allergic to cow’s milk. Unfortunately, potentially harmful bacteria have not been thoroughly tested in goat or sheep milk. Listeria monocytogenes is a harmful bacterium that causes adverse health effects if ingested by humans. The purpose of this study was to estimate the prevalence and characterize the phenotype, genotype, virulence factors, biofilm formation, and antibiopotential of Listeria isolated from the milk of goat and sheep. Udder milk samples were collected from 107 goats and 102 sheep and screened for mastitis using the California mastitis test (CMT). Samples were then examined for the presence of pathogenic Listeria spp; if detected, the isolation of pathogenic Listeria (L. monocytogenes and Listeria ivanovii) was completed using isolation and identification techniques recommended by the International Organization for Standards (ISO 11290-1, 1996), in addition to serological, in vitro and in vivo pathogenicity tests. The isolates were subjected to PCR assay for virulence associated genes (hlyA, plcA, actA, and iap). Pathogenic Listeria spp. were isolated from 5.6% of goat and 3.9% sheep milk samples...

Desempenho materno-filial de ovinos da ra??a Ideal submetidos ?? tosquia pr??-parto.; Desempenho materno-filial de ovinos da ra??a Ideal submetidos ?? tosquia pr??-parto.; Early pre-parturition shearing: performance of mother-offspring in Ideal sheep breed.; Early pre-parturition shearing: performance of mother-offspring in Ideal sheep breed.

SPHOR, Luiza de ??vila
Fonte: Universidade Federal de Pelotas; Zootecnia; Programa de P??s-Gradua????o em Zootecnia; UFPel; BR Publicador: Universidade Federal de Pelotas; Zootecnia; Programa de P??s-Gradua????o em Zootecnia; UFPel; BR
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
46.52%
The increase in consumption and valorization of sheep meat is turning this ranching activity increasingly attractive and profitable. The search for technologies to reduce the loss of lambs by neonatal death is an alternative to increase production levels in sheep raising. The objective of this study was to evaluate the effects of shearing in pregnant ewe on the birth and weaning weights of lambs, as well as on the production and composition of sheep milk. The study was conducted at the Instituto Nacional de Investigaci??n Agropecuaria - INIA, La Estanzuela, Uruguay (34?? 19 S 57 40 N). Twenty Ideal breed ewes, single bearers were allocated to two treatments based on expected day of parturition, body weight and body condition. Ten ewes were shorn at 53 days of gestation and ten ewes were kept unshorn during pregnancy. Milking started seven days after parturition and was repeated weekly during 15 weeks, until weaning. Milk production of ewes shorn during gestation was 22.2% higher compared to unshorn ewes but did not change its percentage of solids. At birth lambs of shorn ewes were 1.4 kg heavier and at weaning this superiority reached 4.5 kg. The pre-parturition shearing at 53 days of gestation is a management tool that can improve the productive characteristics of sheep breed ideal.; O aumento no consumo e na valoriza????o da carne ovina vem tornando essa atividade pecu??ria cada vez mais atrativa e rent??vel. Buscar tecnologias para diminuir a perda de cordeiros ocasionada pela morte neonatal ?? uma alternativa para aumentar os n??veis produtivos da ovinocultura. O presente trabalho teve como objetivo estudar o efeito da tosquia em ovelhas gestantes sobre o peso ao nascimento e ao desmame de seus cordeiros...

The effect of estrus synchronization treatments on somatic cell count of transitional-anestrus Awassi ewes' milk

Talafha, A.; Lafi, S.; Ababneh, M.
Fonte: Kluwer Academic Publ Publicador: Kluwer Academic Publ
Tipo: Artigo de Revista Científica
Publicado em //2009 EN
Relevância na Pesquisa
46.54%
Fifty-three transitional-anestrus Awassi ewes, randomly assigned to three groups: fluorogestone acetate (FGA, n = 18), FGA-Prostaglandin (FGA-PGF, n = 18) and control (n = 17), were used to examine the effect of estrus synchronization protocols and steroid hormones concentrations on milk somatic cell count (SCC). Intravaginal FGA sponge was inserted for 13 days and 600 IU equine chorionic gonadotropin was administered for ewes of FGA and FGA-PGF groups at the time of sponge removal (day 0). In addition, 10 mg was administered to ewes of FGA-PGF group on day 0. Blood and milk samples were collected from all ewes on days -13, -6, 0, 1, 2, 7 and 14. Estradiol had significant positive correlation with the SCC during the periods of sponge insertion (P = 0.015, r = 0.235) and within two days (P = 0.063 r = 0.23) after sponge removal with no correlation with SCC of both udder halves during the luteal phase. Progesterone concentrations, on the other hand, had a significant positive correlation (P < 0.001; r = 0.420) with the SCC of both udder halves during the luteal phase of the experiment, but not during the periods of sponge insertion and expected estrus. SCC returned under the influence of endogenous progesterone on days 7 and 14 to pre-synchronization values. In conclusion...

Comparative evaluation of Portuguese goat and sheep cheeses

Guiné, Raquel; Correia, Paula; Correia, Ana
Fonte: Instituto Politécnico de Viseu Publicador: Instituto Politécnico de Viseu
Tipo: Conferência ou Objeto de Conferência
Publicado em //2014 ENG
Relevância na Pesquisa
46.58%
Cheese is a solid food made by coagulating milk of different animals. It has a great expression worldwide and there is a great diversity in the types of cheese between and within different countries. Maturation (or cure) plays a key role in the development of the individual characteristics of each cheese. Sheep milk is very rich, containing higher amounts of total solids (19.3%), protein (4.5%) and fat (7.4%), of which most are glycerides (98%). Goat's milk is characterized by having 14.1% dry weight, 3.5% fat and 4.5% protein. In this work, six Portuguese cheeses made exclusively from sheep or goat milk were evaluated at the physical (texture and colour), chemical (moisture, aw, ashes protein and fat) and sensory (preference profile) levels. At the chemical level the results show some variability between samples by type of cheese, and the sample of sheep's cheese V presented higher values of protein and fat, 29.7% and 63.5%, respectively. This sample was also at the level of the physical parameters evaluated the one that stood out from the others, being darker (lower L*) and with a greater predominance of red (higher positive a*) and yellow (higher positive b*). With respect to texture, specifically the parameters of hardness, gumminess and chewiness...

Bioaccessibility of Calcium and Phosphorus in Milk Samples.

NOGUEIRA, A. R. de A.; BOSSU, C. M.; MENEZES, E. A.
Fonte: In: EURO ANALYSIS, 2009, Innsbruck. Proceedingis... Innsbruck: ASAC, 2009. 1CD-ROM. Publicador: In: EURO ANALYSIS, 2009, Innsbruck. Proceedingis... Innsbruck: ASAC, 2009. 1CD-ROM.
Tipo: Resumo em anais de congresso (ALICE)
PT_BR
Relevância na Pesquisa
46.31%
Milk is an essential macro and micronutrients source for growth, development and health, and as one of the animal protein sources for ali age's humans. This work aimed to study in vitro bioaccessibility with simulated gastric digestion in the Ca and P levels in samples of different kind of milk (UHT cow's milk, raw cow's milk, raw sheep milk, UHT goat's milk and soya's "milk"). The experiment was based on simulation of gastrointestinal digestion with pepsin-HCI during the gastric stage and pancreaticbiliary salts during the intestinal stage.; 2009

Evaluation of physico-chemical characteristics of fresh, refrigerated and frozen Lacaune ewes' milk

Fava,L.W.; Külkamp-Guerreiro,I.C.; Pinto,A.T.
Fonte: Universidade Federal de Minas Gerais, Escola de Veterinária Publicador: Universidade Federal de Minas Gerais, Escola de Veterinária
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2014 EN
Relevância na Pesquisa
46.32%
The production of ewe milk is seasonal and milk yield per animal is low, even in specialized animals. This study aimed to verify the possibility of preserving bulk tank milk for seven days under cooling (5°C) and freezing (-5°C), verify the influence of cooling treatments and of the months of the year on the physical and chemical characteristics of the product. The chemical composition of milk, including the fat, protein, lactose and total solids contents, was not altered by cooling and freezing. Protein and lactose contents varied according to the months of the year. The average percentage and standard deviation of fat, protein, lactose and total solids was 8.10±1.30, 5.22±0.37, 4.43±0.23 and 19.34±1.54, respectively. The density, pH, titratable acidity, as well as alcohol and heat stability tests were significantly influenced by the treatments used (P < 0.05), but no differences were found between fresh and frozen milk. Prolonged refrigeration caused an increase in acidity and decrease in pH, with a consequent reduction in the stability of milk. These results demonstrated that freezing does not affect the chemical composition and physical characteristics of milk in nature and it could be a solution for the producer and the sheep milk industry.

Milk Production, Conjugated Linoleic Acid Content, and In Vitro Ruminal Fermentation in Response to High Levels of Soybean Oil in Dairy Ewe Diet

Gómez-Cortés, Pilar; Frutos, Pilar; Mantecón, Ángel R.; Juárez, Manuela; Fuente, Miguel Ángel de la; Hervás, Gonzalo
Fonte: American Dairy Science Association Publicador: American Dairy Science Association
Tipo: Artículo Formato: 115948 bytes; application/pdf
ENG
Relevância na Pesquisa
46.48%
10 pages, 5 tables, 1 figure.-- PMID: 18349249 [PubMed].-- © 2008 American Dairy Science Association.; Previously published as proceeding at the XXXVIII Jornadas de Estudio AIDA (XII Jornadas sobre Producción Animal) (Zaragoza, Spain, May 16-17, 2007). https://digital.csic.es/handle/10261/4374; Feeding vegetable oils rich in linoleic acid has been demonstrated to be an effective strategy to enrich milk with conjugated linoleic acid (CLA). However, high amounts of vegetable oil in the diet in free form could adversely affect animal performance, mainly in sheep. The aim of this work was to improve the ewe milk fatty acid profile by increasing potentially healthy acids such as CLA without any detrimental effects on milk production and ruminal fermentation with soybean oil (SBO) diet supplementation. Twenty-four ewes were assigned to 2 treatments and fed 2 diets (control or supplemented with 6% of SBO; 2 lots of 6 animals per treatment) and fed ad libitum for 4 wk. The forage:concentrate ratio was 20:80. Batch cultures of rumen microorganisms were used to study in vitro rumen fermentation. Changes in fatty acid profile were characterized as a reduction in C6:0 to C16:0 at the expense of an increase in C18:0, C18:1 isomers, and CLA concentrations. Proportions of milk CLA and trans-11 C18:1 (vaccenic acid) went from 1.04 to 3.44 and 2.08 to 6.20 g/100 g of total fatty acids...

Dietary effect of silage type and combination with camelina seed on milk fatty acid profile and antioxidant capacity of sheep milk

Mierlita,D.; Vicas,S.
Fonte: South African Journal of Animal Science Publicador: South African Journal of Animal Science
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2015 EN
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46.35%
The present study sought to quantify the differences between maize-based (MS) and grass-silage-based (GS) diets in terms of their effect on the milk yield, milk fatty acid composition and antioxidant capacity in dairy ewes, and to test the hypothesis that it is possible to improve yield, fatty acid (FA) composition and antioxidant capacity by supplementing diet with camelina seed (Cs). Experimental diets consisted of a 2 x 2 factorial arrangement of type of silage (GS vs. MS) and camelina seed (-Cs vs. +Cs). Feeding the MS diets increased net energy for lactation (NE L) intake, raw milk yield and fat, protein and lactose yields. Feeding +Cs increased energy corrected milk (ECM), milk fat content and fat yield. Maize silage consumption is associated with an increased proportion of hypercholesterolemic fatty acids (HFA) and a higher value of the atherogenicity index. However, an MS diet led to an increased share of t11-C18:1 and c9,t11-conjugated linoleic acid (CLA) in milk. Milk FA profile in ewes fed GS diet was of higher quality for human beings owing to higher concentrations of α-linolenic acid and a lower content of HFA. Supplementing with camelina seed resulted in a higher concentration of t11-C18:1, c9,t11-CLA and C18:3n-3 in milk fat. The trolox equivalent antioxidant capacity (TEAC) value of milk was higher in milk from MS-fed ewes compared with that of their counterparts fed GS. Dietary supplementation with camelina seed increased the oxidative stability of milk samples. These results suggest that grass-silage-based diet supplemented with camelina seed results in milk of better quality for human consumption.