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Mastitis diagnosis in dairy goats through somatic cell counts and California mastitis test. Preliminary results

Mendonça, Álvaro; Nunes, Mário Armada; Valentim, Ramiro; Correia, Teresa Montenegro; Trigo, Margarida; Maurício, Raimundo; Costa, Cristina; Coelho, Alípio
Fonte: CIHEAM Publicador: CIHEAM
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
86.04%
The aim of this work was to evaluate somatic cell count (SCC) and Californian mastitis test (CMT) reliability as methods to survey mastitis in Serrana goats. Microbiological diagnosis, SCC and CTM were performed on 2028 samples, collected from individual glands during a lactation period. According to results CMT (predictive negative value = 69.5%) may be used as a cheap and practical method for sub clinical mastitis survey in Serrana goats. Decision on SCC use will depend on additional research works, since its values were very high even for bacteriological negative samples.

Efficiency of somatic cell count and california mastitis test in the diagnosis of subclinical mastitis in terrincha ewes

Mendonça, Álvaro; Machado, M.; Tavares, A.; Quintas, Hélder; Valentim, Ramiro; Maurício, Raimundo; Cardoso, Manuel
Fonte: Associação Portuguesa de Buiatria Publicador: Associação Portuguesa de Buiatria
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
86.22%
This study aimed to compare the efficiency of microbiological test with Californian Mastitis Test and somatic cell count in the diagnosis of Subclinical Mastitis (SM) in Terrincha sheep. Twenty-seven of a flock of about 200 Terrincha ewes (local breed) were studied for a period of 9 weeks (n > 497 samples). Milk samples were aseptically collected from each half udder once a week. At the same time, another sampled was collected from the bulk tank. After being transported to Lab under refrigeration all samples were immediately processed. The tests performed were the total microbial count (PCA), the Californian Mastitis Test (CMT) and the somatic cell count (SCC). After PCA testing, all samples exceeding 500 cfu/ml of milk (10-1 dilution) were considered positive to mastitis. The SCC was performed by a Fossmatic equipment at the Lactogal Lab. CMT was more accurate to predict Negative (87.1%) than Positive (43.1%) samples (Chi-square = 42.5; P≤0.001), meaning that 12.9% half udders were classified as negative being positive and 47.7% half udders were classified as positive being negative. PCA Negative and Positive samples were related to different SCC values (Negative: 277,048.9 ± 571,249.7 vs. Positive: 800,329.5 ± 1,444.970 somatic cells; P≤0.001)...

Short communication: Evaluation of an on-farm test to estimate somatic cell count

RODRIGUES, A. C. O.; CASSOLI, L. D.; MACHADO, P. F.; RUEGG, P. L.
Fonte: AMER DAIRY SCIENCE ASSOC-ADSA Publicador: AMER DAIRY SCIENCE ASSOC-ADSA
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.15%
The objective of this study was to compare the results of an on-farm test, named Somaticell, with results of electronic cell counting and for milk somatic cell count (SCC) among readers. The Somaticell test correctly determined the SCC in fresh quarter milk samples. Correlation between Somaticell and electronic enumeration of somatic cells was 0.92 and. coefficient 0.82. Using a threshold of 205,000 cells/mL, the sensitivity and specificity for determination of intramammary infections were 91.3 and 96.0%, respectively. The SCC was greater for milk samples from which major mastitis pathogens were recovered. Minor variation among readers was observed and most likely associated with the mixing procedure. However, the final analysis indicated that this variation was not significant and did not affect the amount of samples classified as having subclinical mastitis. The on-farm test evaluated in this study showed adequate capacity of determining SCC on quarter milk samples and may be considered as an alternative for on-farm detection of subclinical mastitis.; Madasa (Sao Paulo, Brazil)

Apoptosis and necrosis of polymorphonuclear leukocytes in goat milk with high and low somatic cell counts

Blagitz, Maiara Garcia; Souza, Fernando Nogueira; Gomes, Viviani; Libera, Alice Maria Melville Paiva Della
Fonte: Elsevier Science Bv Publicador: Elsevier Science Bv
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
86.09%
The purpose of the present trial was to compare the percentages of necrotic and apoptotic polymorphonuclear leukocytes (PMNL) in goat milk with low and high somatic cell count (SCC). Twenty eight milk samples were collected from 20 lactating goats, determined to be negative in bacteriological examination, and divided in three groups, according to their SCC: samples with SCC lower than 500 x 10(3) cells/mL; between 500 and 1500 x 10(3) cells/mL; and higher than 1500 x 10(3) cells/m L. SCC was performed in an automatic somatic cell counter. Apoptosis and necrosis were quantified using dual-color flow cytometry with fluorescein labeled annexin-V and propidium iodide (PI). Results of the present study showed a significant positive correlation between the percentage of the viable PMNL and milk SCC(r = 0.495, P=0.008), as well as a significant negative correlation between apoptotic PMNL and milk SCC(r = -0.486, P = 0.009). Results also pointed out lower PMNL viability rates due to higher apoptosis rates in milk samples with SCC lower than 5 x 10(5) cells/mL. (C) 2011 Elsevier B.V. All rights reserved.

Microbial and sensory changes throughout the ripening of Prato cheese made from milk with different levels of somatic cells

VIANNA, P. C. B.; MAZAL, G.; SANTOS, M. V.; BOLINI, H. M. A.; GIGANTE, M. L.
Fonte: AMER DAIRY SCIENCE ASSOC Publicador: AMER DAIRY SCIENCE ASSOC
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
76.21%
The objective of this research was to evaluate the effects of 2 levels of raw milk somatic cell count (SCC) on the composition of Prato cheese and on the microbiological and sensory changes of Prato cheese throughout ripening. Two groups of dairy cows were selected to obtain low-SCC (<200,000 cells/mL) and high-SCC (>700,000 cells/mL) milks, which were used to manufacture 2 vats of cheese. The pasteurized milk was evaluated according to the pH, total solids, fat, total protein, lactose, standard plate count, coliforms at 45 degrees C, and Salmonella spp. The cheese composition was evaluated 2 d after manufacture. Lactic acid bacteria, psychrotrophic bacteria, and yeast and mold counts were carried out after 3, 9, 16, 32, and 51 d of storage. Salmonella spp., Listeria monocytogenes, and coagulase-positive Staphylococcus counts were carried out after 3, 32, and 51 d of storage. A 2 x 5 factorial design with 4 replications was performed. Sensory evaluation of the cheeses from low- and high-SCC milks was carried out for overall acceptance by using a 9-point hedonic scale after 8, 22, 35, 50, and 63 d of storage. The somatic cell levels used did not affect the total protein and salt: moisture contents of the cheeses. The pH and moisture content were higher and the clotting time was longer for cheeses from high-SCC milk. Both cheeses presented the absence of Salmonella spp. and L. monocytogenes...

Effect of somatic cell count and mastitis pathogens on milk composition in Gyr cows

Malek dos Reis, Carolina Barbosa ; Barreiro, Juliana Regina; Mestieri, Lucineia; Porcionato, Marco Aurélio de Felício ; Santos, Marcos Veiga dos
Fonte: London Publicador: London
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
96.28%
Abstract Background Gyr cows are well adapted to tropical conditions, resistant to some tropical diseases and have satisfactory milk production. However, Gyr dairy herds have a high prevalence of subclinical mastitis, which negatively affects their milk yield and composition. The objectives of this study were (i) to evaluate the effects of seasonality, mammary quarter location (rear x front), mastitis-causing pathogen species, and somatic cell count (SCC) on milk composition in Gyr cows with mammary quarters as the experimental units and (ii) to evaluate the effects of seasonality and somatic cell count (SCC) on milk composition in Gyr cows with cows as the experimental units. A total of 221 lactating Gyr cows from three commercial dairy farms were selected for this study. Individual foremilk quarter samples and composite milk samples were collected once a month over one year from all lactating cows for analysis of SCC, milk composition, and bacteriological culture. Results Subclinical mastitis reduced lactose, nonfat solids and total solids content...

Correlação entre ácido ascórbico plasmático, contagem de células somáticas no leite e o perfil metabólico de vacas secas e em lactação; Correlation between plasma ascorbic acid, milk somatic cell count and metabolic profile in lactating and dry cows

Santos, Marcos Veiga dos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 26/10/1998 PT
Relevância na Pesquisa
96.1%
Vacas em lactação apresentam capacidade de síntese endógena de ácido ascórbico para suprir os seus requerimentos, no entanto, sob condições estressantes como altas temperaturas e umidade, elevadas produções de leite, parasitoses e incidência de doenças, pode haver produção insuficiente de ácido ascórbico para as demandas metabólicas do animal. Foram objetivos do presente estudo avaliar o efeito do estágio de lactação e número de lactações sobre a concentração plasmática de ácido ascórbico de 153 vacas em lactação e 40 vacas no período seco em 3 fazendas leiteiras, e sua correlação com: a contagem de células somáticas (CCS) no leite, níveis de glicose plasmática, níveis de ácidos graxos não-esterificados (AGNE), níveis de insulina plasmática, níveis de beta-hidroxibutirato (BHBA) plasmático, níveis de aspartato-aminotransferase (AST) plasmática, níveis de produção de leite e escore de condição corporal. Os animais foram escolhidos ao acaso e agrupados em 5 grupos de acordo com o estágio de lactação (estágio 1: 1-28 dias; estágio 2: 29-56 dias; estágio 3: 57-140 dias; estágio 4: 141-280 dias e estágio 5: vacas secas) e de acordo com o número de lactações (primíparas ou multíparas). Amostras de sangue foram coletadas para determinação de ácido ascórbico plasmático utilizando técnica de cromatografia líquida de alta pressão. A análise estatística foi realizada com o uso do programa computacional SAS. A concentração média (mg/L) de ácido ascórbico plasmático foi de 2...

Avaliação da contagem de células somáticas do leite como indicador da ocorrência de mastite em vacas Gir; Evaluation of milk somatic cell count as an indicator of mastitis occurrence in Gyr cows

Reis, Carolina Barbosa Malek dos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 31/03/2010 PT
Relevância na Pesquisa
96.1%
Os objetivos deste trabalho foram determinar a sensibilidade e especificidade do limiar de contagem de células somáticas (CCS) de vacas Gir para o diagnóstico de mastite subclínica causada por patógenos primários e secundários e avaliar os efeitos de rebanho, vaca, mês de coleta, quarto mamário, presença de infecção intramamária, tipo de microrganismo e suas interações sobre o logCCS e composição do leite. Avaliou-se a hipótese que o limiar da CCS para detecção de mastite subclínica é igual entre vacas Gir e Holandesas. Foram utilizadas 221 vacas Gir em lactação, provenientes de três fazendas comerciais. Foram coletadas amostras de leite individuais por quarto mamário e compostas uma vez por mês, durante um ano. Foram realizadas análises de CCS, composição do leite e cultura microbiológica. O quarto mamário e a vaca foram considerados unidades experimentais. Para determinar a sensibilidade, especificidade e odds ratio (OR) dos limiares da CCS para identificação de quartos infectados, foram utilizados quatro valores de CCS: 100, 200, 300 e 400 (x 103 células/mL), assim como a correlação entre a CCS e composição do leite. Não houve efeito do rebanho sobre o logCCS para amostras individuais de quartos mamários e compostas...

Somaticell® as a screening method for somatic cell count from bovine milk

Langoni, Helio; Penachio, Daniel da Silva; Nóbrega, Diego Borin; Guimarães, Felipe de Freitas; Lucheis, Simone Baldini
Fonte: Universidade Federal de Santa Maria (UFSM) Publicador: Universidade Federal de Santa Maria (UFSM)
Tipo: Artigo de Revista Científica Formato: 1095-1101
ENG
Relevância na Pesquisa
96.18%
Os objetivos do presente estudo foram avaliar a correlação entre a contagem eletrônica de células somáticas (eCCS) com o Somaticell® sob diferentes níveis de contagem de células somáticas (CCS) do leite e patógenos causadores de mastites, além de calcular a sensibilidade, especificidade e valores preditivos do Somaticell® utilizando diferentes limites de CCS estabelecidos pelos diferentes países. Trezentos e quarenta amostras de leite foram coletadas assepticamente após realização do California Mastitis Test (CMT). O Somaticell® e a eCCS foram realizados em todas as amostras de leite. A correlação entre o Somaticell® e a contagem eletrônica foi determinada de acordo com o CMT, patógeno isolado e escore de eCCS. de acordo com os escores de CCS estabelecidos, 26,5% das amostras de leite apresentaram escore 1 (69-166 x10³células mL-1), 26,8% escore 2 (167-418x10³células mL-1), 27,4% escore 3 (419-760x10³células mL-1) e 19,4% escore 4 (761 to 1970x10³células mL-1). A eCCS e o Somaticell® apresentaram correlação positiva em quase todos os escores estudados (exceto escore 2 e 3). O valor de r obtido entre CCS e o Somaticell® foi de 0,32. Observou-se que, quando o limite de CCS estabelecido aumentou, a sensibilidade decresceu e os valores de especificidade aumentaram. Os valores preditivos apresentaram-se constantes em todos os limites. Quando o limite de CCS era baixo (<760...

Efeito da contagem de celulas somaticas do leite na fabricação do queijo prato.; Effect of the somatic cell count on the manufacture of the prato cheese.

Guillaume Mazal
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 08/08/2005 PT
Relevância na Pesquisa
96.2%
A mastite é definida como uma inflamação do úbere e caracteriza-se pelo aumento de número de células somáticas no leite. O processo inflamatório provoca alterações da composição do leite e da atividade enzimática, o que dá origem a produtos lácteos de qualidade inferior. O objetivo do trabalho foi avaliar o efeito da contagem de células somáticas (CCS) do leite sobre a sua composição, o efeito do tratamento (CCS e tempo de coagulação do leite) sobre a composição do soro e do queijo, assim como, o rendimento e a maturação do queijo. Inicialmente, selecionaram-se dois grupos de animais para obtenção de leite com concentração de células somáticas baixa (< 200.000 cél/mL) e alta (> 600.000 cél/mL). Os leites foram submetidos a três tratamentos para obtenção de queijo Prato: (1) obtido a partir de leite com CCS baixa e coagulação em 35 minutos; (2) obtido a partir de leite com CCS alta e coagulação em 35 minutos; (3) obtido a partir de leite com CCS alta e tempo de coagulação ajustado. Avaliaram-se as composições dos leites, soros e queijos e calculou?se o rendimento e a recuperação dos constituintes do leite para o queijo. Os queijos foram avaliados após 5, 12, 19, 26, 33 e 40 dias de maturação quanto ao pH...

Microbial and sensory changes throughout the ripening of Prato cheese made from milk with different levels of somatic cells

VIANNA, P. C. B.; MAZAL, G.; SANTOS, M. V.; BOLINI, H. M. A.; GIGANTE, M. L.
Fonte: AMER DAIRY SCIENCE ASSOC Publicador: AMER DAIRY SCIENCE ASSOC
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
76.19%
The objective of this research was to evaluate the effects of 2 levels of raw milk somatic cell count (SCC) on the composition of Prato cheese and on the microbiological and sensory changes of Prato cheese throughout ripening. Two groups of dairy cows were selected to obtain low-SCC (<200,000 cells/mL) and high-SCC (>700,000 cells/mL) milks, which were used to manufacture 2 vats of cheese. The pasteurized milk was evaluated according to the pH, total solids, fat, total protein, lactose, standard plate count, coliforms at 45 degrees C, and Salmonella spp. The cheese composition was evaluated 2 d after manufacture. Lactic acid bacteria, psychrotrophic bacteria, and yeast and mold counts were carried out after 3, 9, 16, 32, and 51 d of storage. Salmonella spp., Listeria monocytogenes, and coagulase-positive Staphylococcus counts were carried out after 3, 32, and 51 d of storage. A 2 x 5 factorial design with 4 replications was performed. Sensory evaluation of the cheeses from low- and high-SCC milks was carried out for overall acceptance by using a 9-point hedonic scale after 8, 22, 35, 50, and 63 d of storage. The somatic cell levels used did not affect the total protein and salt: moisture contents of the cheeses. The pH and moisture content were higher and the clotting time was longer for cheeses from high-SCC milk. Both cheeses presented the absence of Salmonella spp. and L. monocytogenes...

Somaticell® as a screening method for somatic cell count from bovine milk

Langoni,Hélio; Penachio,Daniel da Silva; Nóbrega,Diego Borin; Guimarães,Felipe de Freitas; Lucheis,Simone Baldini
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2012 EN
Relevância na Pesquisa
96.18%
The objectives of the present study were to evaluate the correlation between electronic somatic cell count (eSCC) and Somaticell® under different milk somatic cell count (SCC) conditions and to different mastitis pathogens and calculate the, sensitivity, specificity and predictive values of Somaticell® using different SCC limits established by different countries. Three-hundred and forty milk samples were aseptically collected according to the California Mastitis Test (CMT) result. The Somaticell® and eSCC were carried out in all milk samples. The correlation between Somaticell® test results and electronic counts was determined according to the CMT, isolated pathogen and eSCC score. According to the SCC scores established, 26.5% milk samples showed score 1 (69-166x10³cells mL-1), 26.8% score 2 (167-418x10³cells mL-1), 27.4% score 3 (419-760x10³cells mL-1) and 19.4% score 4 (761 to 1,970x10³cells mL-1). According to Spearmann correlation test, eSCC and Somaticell® had a positive correlation (P<0.05) in almost all conditions (except eSCC score 2 and score 3). The r value obtained between the SCC and Somaticell® was 0.32. It was observed that as the SCC thresholds increased, the sensitivity values decrease and specificity increased. The predictive values remained constant among all limits. When the SCC limit is lower (<760...

Management factors and cow traits influencing milk somatic cell counts and teat hyperkeratosis during different seasons

Sandrucci,Anna; Bava,Luciana; Zucali,Maddalena; Tamburini,Alberto
Fonte: Sociedade Brasileira de Zootecnia Publicador: Sociedade Brasileira de Zootecnia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2014 EN
Relevância na Pesquisa
86.28%
The objective of the study was to analyze the effect of season, parity, stage of lactation and milking procedures on teat-end condition, cow cleanliness and milk somatic cell count (SCC) and identify risk factors associated with milk somatic cell counts greater than 100,000 cells/mL. A group of 15 Italian dairy farms were visited three times during different seasons: the cold (3.8 °C), the hot (23.5 °C) and the mild (12.1 °C) seasons. Hygiene of udder, flanks and legs was scored on 2,330 cows based on a 4-point scale system, from very clean (score 1) to very dirty skin (score 4). On the same cows, a total of 9,201 teats were assessed for teat-end condition and assigned to four different classes of hyperkeratosis: No lesion (N), Smooth ring (S), Rough (R) and Very rough skin (VR). The average percentage of teats classified in the worst classes of hyperkeratosis (R and VR) equaled 15.9%. Teat hyperkeratosis, cow cleanliness and milk somatic cell count were significantly affected by the season. Teat condition was significantly better in primiparous than in multiparous cows and deteriorated during lactation. Cows with the lowest values of SCC, better teat conditions and better hygiene scores were found in the farms where more than one milking practice (among forestripping...

Avaliação de métodos de análises para determinação da contagem de células somáticas no leite cru, mantido em tanque de resfriamento; Evaluation of methods of analysis to determine the somatic cell count in raw milk, kept in the cooling tank

Pereira Neto, Manoel
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Produção Animal; Sistemas de Produção Sustentáveis no Semi-árido; Caracterização, conservação e melhoramento genético Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Produção Animal; Sistemas de Produção Sustentáveis no Semi-árido; Caracterização, conservação e melhoramento genético
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
86.32%
We analyzed the quality of raw milk from eight dairy farms in Rio Grande do Norte stored in a cooling tank , in order to evaluate methods for determining somatic cell counts (SCC). The Somaticell® kit and a portable Direct Cell Counter (DCC) were compared with each other and with the MilkoScanTM FT+ (FOSS Denmark), which uses Fourier Transform Infrared (FTIR) spectroscopy). Direct cell counter data were processed for somatic cell scores (log-transformed somatic cell count) and analyzed with the SAS®, statistical package , Statistical Analysis System, (SAS, INSTITUTE, 1998). Comparison of means and correlation of somatic cell scores were conducted using Pearson s correlation coefficient and the Tukey Test at 1 %. No significant difference was observed for comparison of means. The correlation between somatic cell scores was significant, that is, 0.907 and 0.876 between the MilkoScanTM FT+ and the Somaticell® kit and Direct Cell Count (DCC) respectively, and 0.943 between the Somaticell® kit and Direct Cell Count (DCC). The methods can be recommended for monitoring the quality of raw milk kept in a cooling tank in the production unit; Conselho Nacional de Desenvolvimento Científico e Tecnológico; A qualidade do leite cru mantido em tanque de resfriamento de oito propriedades do Rio Grande do Norte foi analisada...

The effect of estrus synchronization treatments on somatic cell count of transitional-anestrus Awassi ewes' milk

Talafha, A.; Lafi, S.; Ababneh, M.
Fonte: Kluwer Academic Publ Publicador: Kluwer Academic Publ
Tipo: Artigo de Revista Científica
Publicado em //2009 EN
Relevância na Pesquisa
96.1%
Fifty-three transitional-anestrus Awassi ewes, randomly assigned to three groups: fluorogestone acetate (FGA, n = 18), FGA-Prostaglandin (FGA-PGF, n = 18) and control (n = 17), were used to examine the effect of estrus synchronization protocols and steroid hormones concentrations on milk somatic cell count (SCC). Intravaginal FGA sponge was inserted for 13 days and 600 IU equine chorionic gonadotropin was administered for ewes of FGA and FGA-PGF groups at the time of sponge removal (day 0). In addition, 10 mg was administered to ewes of FGA-PGF group on day 0. Blood and milk samples were collected from all ewes on days -13, -6, 0, 1, 2, 7 and 14. Estradiol had significant positive correlation with the SCC during the periods of sponge insertion (P = 0.015, r = 0.235) and within two days (P = 0.063 r = 0.23) after sponge removal with no correlation with SCC of both udder halves during the luteal phase. Progesterone concentrations, on the other hand, had a significant positive correlation (P < 0.001; r = 0.420) with the SCC of both udder halves during the luteal phase of the experiment, but not during the periods of sponge insertion and expected estrus. SCC returned under the influence of endogenous progesterone on days 7 and 14 to pre-synchronization values. In conclusion...

The effect of estus synchronization treatment on somatic cell count of transitional-anestrus local-Damascus cross breed goats' milk

Talafha, A.; Lafi, S.; Ababneh, M.
Fonte: Kluwer Academic Publ Publicador: Kluwer Academic Publ
Tipo: Artigo de Revista Científica
Publicado em //2008 EN
Relevância na Pesquisa
96.1%
An experiment was conducted to examine the effect of estrus synchronization protocols and steroid hormones concentrations on somatic cell count (SCC) of transitional-anestrus local-Damascus cross goats’ milk. Fifty-six goats (2–4-year old) were randomly assigned to three groups: fluorogestone acetate (FGA, n = 19), FGA-Prostaglandin (FGA-PGF, n = 19) and control (n = 18) groups. Intravaginal sponge containing 40 mg FGA was inserted for 13 days and an injection of 600 IU equine chorionic gonadotropin (eCG) was administered for goats of FGA and FGA-PGF groups at the time of sponge removal (day 0). In addition, goats of FGA-PGF group were injected with 10 mg dinoprost tromethamine (PGF₂α) on day 0. Five fertile local-Damascus cross bucks were turned-in with all goats on day 0. Blood and milk samples were collected from all goats on days -13 (beginning of experiment), -6, 0, 1, 2, 7, 13 and 20 (end of the experiment). Four-year old and second-parity goats had significantly higher (p < 0.05) SCC of both udder halves than 2- and 3-year old and first-parity goats, respectively. There was a significant effect (p < 0.05) for treatment and number of kids born in the last kidding season on SCC of both udder halves. Neither estradiol nor progesterone concentrations were correlated with SCC in goats in this experiment. The SCC of both udder halves and left udder halves in goats of the control and FGA groups...

Relationship between total bacteria counts and somatic cell counts from mammary quarters infected by mastitis pathogens

Lopes Júnior,João Emídio Ferreira; Lange,Carla Cristine; Brito,Maria Aparecida Vasconcelos Paiva; Santos,Fabiana Ribeiro; Silva,Marco Aurélio Souto; Moraes,Luciano Castro Dutra de; Souza,Guilherme Nunes de
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2012 EN
Relevância na Pesquisa
86.1%
This study was conducted to establish the relationship between somatic cell count (SCC) and bacterial shedding from mammary quarters according to mastitis pathogens. Milk samples from 638 mammary quarters were examined for mastitis pathogens, SCC and total bacterial count (TBC). The raw data of SCC and TBC were used to perform descriptive statistics. The significance of the arithmetic mean differences between SCC and TBC according to bacteriological examination results was determined by a two-tailed unpaired t-test. Pearson and Spearman´s correlations were done with logarithmic data and linear regression analyses. The geometric means of the bacteriological examination results were (cells mL-1; CFU mL-1): no growth (52,000; 12,000), coagulase-negative staphylococci (85,000; 17,000), Staphylococcus aureus (587,000; 77,000); other streptococci (432,000; 108,000) and Streptococcus agalactiae (1,572,000; 333,000). The Pearson and Spearman's correlations between SCC and TBC were higher than 0.60 for all mastitis pathogens. The regression analyses slopes showed different increase in TBC with the same increase in SCC according to mastitis pathogens. The slope for S. agalactiae (0.542) was higher than that for other mastitis pathogens. The results suggest that the intensity of inflammatory process was associated with number of mastitis pathogens shedding from the mammary gland.

Composição e contagem de células somáticas no leite de rebanhos caprinos do Sudeste do Brasil; Composition and bulk tank somatic cell counts of milk from dairy goat herds in Southeastern Brazil

Souza, Guilherme Nunes de; Brito, José Renaldi Feitosa; Brito, Maria Aparecida Vasconcelos Paiva; Lange, Carla; Faria, Cristiano Gomes de; Moraes, Luciano Castro Dutra de; Fonseca, Rafael Guedes; Silva, Yuri de Almeida
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; ; Formato: application/pdf
Publicado em 01/02/2009 ENG
Relevância na Pesquisa
86.15%
A composição do leite bem como a contagem de células somáticas (CCS) são requisitos para avaliar a qualidade do leite e mastite em rebanhos caprinos. Estudos conduzidos indicaram que a composição do leite varia entre os rebanhos devido a fatores genéticos, alimentação, sistema de produção, estágio de lactação, ano e estação do ano. O objetivo do estudo foi avaliar a CCS e outros indicadores de qualidade (gordura, proteína, lactose e sólidos totais) no leite de rebanhos caprinos. A influência do rebanho e estação do ano sobre a composição bem como a influência de rebanho, tipo de ordenha e estação do ano sobre a CCS também foi avaliado. Treze rebanhos caprinos localizados no Brasil, com aproximadamente 1.400 matrizes foram incluídos no estudo, Sendo ordenhados manualmente e os outros sete com equipamento de ordenha. As amostras de leite dos rebanhos foram coletadas semanalmente durante duas lactações. O total de 913 amostras foi analisado no equipamento automatizado. A média dos valores percentuais para gordura, proteína, lactose e sólidos totais foram, respectivamente; 3,44; 2,95; 4,45 e 11,69. O efeito de rebanho e estação do ano foi significante para todos os componentes do leite e CCS. A média para CCS de todos 13 rebanhos foi 779.000 células/ml. As médias para CCS dos rebanhos ordenhados manualmente e mecanicamente foram...

Efeito do nível de células somáticas sobre os constituintes do leite II-lactose e sólidos totais; Effects of somatic cell levels on milk components II-lactose and total solids

Prada e Silva, Luís Felipe; Pereira, Alfredo Ribeiro; Machado, Paulo Fernando; Sarriés, Gabriel Adrián
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/2000 POR
Relevância na Pesquisa
86.15%
Em laboratório foram determinadas eletronicamente a contagem de células somáticas (CCS) e as concentrações de lactose e sólidos totais de 1.361 amostras de leite com o objetivo de verificar como a concentração desses componentes do leite é alterada pelo aumento da CCS. A CCS foi transformada para escore linear. O aumento do escore levou a uma redução da concentração de lactose, mas não alterou a concentração de sólidos totais. A redução da concentração de lactose foi altamente significativa (p < 0,001), e aproximadamente 34% da variação de sua concentração podem ser explicados pelo aumento do escore linear.; In laboratory, electronic determination of somatic cell count (SCC) and concentration of lactose and total solids of 1,361 milk samples were carried out in order to evaluate the effects of somatic cell count (SCC) on the concentrations of milk lactose and total solids. All the SCC data were transformed to a linear score. Increases in the SCC score were associated with decreased lactose concentration. This increase was highly significant (p < 0.0001), and about 34% of the lactose concentration variation could be accounted for variation of the SCC score. Total solids were not influenced by the SCC score.

Efeito do nível de células somáticas sobre os constituintes do leite I-gordura e proteína; Effects of somatic cell levels on milk components I-fat and protein

Pereira, Alfredo Ribeiro; Prada e Silva, Luís Felipe; Molon, Leonardo Kehdi; Machado, Paulo Fernando; Barancelli, Giovana
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/1999 POR
Relevância na Pesquisa
86.04%
Foram analisadas eletronicamente 6.112 amostras de leite para CCS e concentração de gordura e proteína com o objetivo de verificar como estes teores são alterados pelo aumento da CCS. A CCS foi transformada para escore linear. O aumento do escore levou a um aumento da concentração de gordura, e a um aumento da concentração de proteína. Estes aumentos foram altamente significativos (p<0,0001), porém apenas 6,97% da variação da concentração de gordura e 12,3% da variação da concentração de proteína foram devidos à variação das CCS.; To evaluate the effects of somatic cell count (SCC) on the concentrations of milk fat and protein, eletronic analysis of 6112 samples were carried out at the Laboratory of Lactation Phisiology from the Department of Zoology, ESALQ - Univesity of São Paulo. All the SCC data were transformed to a linear score. Increases in the SCC score were associated with increases in fat and protein concentrations. This relationship was highly significant (p<0.0001), but only 6.97% of the fat concentration and 12.3 % of the protein concentration could be accounted for variation of the SCC.