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Specific sublingual immunotherapy with peach LTP (Pru p 3). One year treatment: a case report

Pereira, C; Bartolomé, B; Asturias, JA; Ibarrola, I; Tavares, B; Loureiro, G; Machado, D; Chieira, C
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em //2009 ENG
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INTRODUCTION: Food allergy is an increasing problem with limited therapeutic approaches apart from to the eviction diet. CASE PRESENTATION: A 40-year-old female patient with food allergy symptoms was polysensitized to almost all vegetable food since the age of 36; the onset of symptoms was during pregnancy. The allergological study demonstrated positive skin prick tests (SPT) to nuts, legumes, cereals, spices, several fresh fruits including peach, and other groups of vegetable foods however, it was negative to common aeroallergens. Serum specific IgE levels were negative (<0.35 kU/L) to profilin and carbohydrate determinants, but positive to Pru p 3 (3.5 kU/L). Positive double-blind placebo-controlled food challenge to peach confirmed the allergic disease. She received specific sublingual immunotherapy with native Pru p 3 at a concentration of 40 mug/ml with 5 administrations per week and a cumulative dose of 200 mug of nPru p 3 per month. After an ultra-rush build-up phase concluded in one day she continued therapy during a year with 5 administrations per week. The clinical evolution and laboratory studies demonstrated an early reduction on SPT reactions with no relevant changes on serum specific IgE, IgG, IgG(1) and IgG(4) to Pru p 3 during the immunotherapy period. The challenge test was negative 4 months after the beginning of the SLIT. Regarding clinical response she markedly improved after the first month of treatment...

Specific sublingual immunotherapy with peach LTP (Pru p 3). One year treatment: a case report

Pereira, Celso; Bartolomé, Borja; Asturias, Juan Andrés; Ibarrola, Iñaki; Tavares, Beatriz; Loureiro, Graça; Machado, Daniel; Chieira, Celso
Fonte: Cases Network Ltd Publicador: Cases Network Ltd
Tipo: Artigo de Revista Científica
Publicado em 12/05/2009 EN
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FAST: towards safe and effective subcutaneous immunotherapy of persistent life-threatening food allergies

Zuidmeer-Jongejan, Laurian; Fernandez-Rivas, Montserrat; Poulsen, Lars K; Neubauer, Angela; Asturias, Juan; Blom, Lars; Boye, Joyce; Bindslev-Jensen, Carsten; Clausen, Michael; Ferrara, Rosa; Garosi, Paula; Huber, Hans; Jensen, Bettina M; Koppelman, Stef;
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 09/03/2012 EN
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The FAST project (Food Allergy Specific Immunotherapy) aims at the development of safe and effective treatment of food allergies, targeting prevalent, persistent and severe allergy to fish and peach. Classical allergen-specific immunotherapy (SIT), using subcutaneous injections with aqueous food extracts may be effective but has proven to be accompanied by too many anaphylactic side-effects. FAST aims to develop a safe alternative by replacing food extracts with hypoallergenic recombinant major allergens as the active ingredients of SIT. Both severe fish and peach allergy are caused by a single major allergen, parvalbumin (Cyp c 1) and lipid transfer protein (Pru p 3), respectively. Two approaches are being evaluated for achieving hypoallergenicity, i.e. site-directed mutagenesis and chemical modification. The most promising hypoallergens will be produced under GMP conditions. After pre-clinical testing (toxicology testing and efficacy in mouse models), SCIT with alum-absorbed hypoallergens will be evaluated in phase I/IIa and IIb randomized double-blind placebo-controlled (DBPC) clinical trials, with the DBPC food challenge as primary read-out. To understand the underlying immune mechanisms in depth serological and cellular immune analyses will be performed...

Severe peach allergy in patients non-sensitized to Pru p 3

Leoni, MC; Caimmi, D; Chiriac, AM; Demoly, MP; Demoly, P
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 25/07/2013 EN
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Prevalence and clinical relevance of low levels of Pru p 3 specific IgE in patients with peach sensitization

Pascal, M; Mensa, A; Sánchez-López, J; Milà, J; Millan, L; Juan, M; Valero, A; Vilella, R; Picado, C; Yagüe, J; Bartra, J
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 25/07/2013 EN
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35.72%

Frequency of LTP (Pru p 3) and profilin (Pru p 4) sensitization in 1052 patients referenced to an Imunoallergology Department in Lisbon

Silva, PM; Pestana, L; Costa, AC; Barbosa, MP
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 25/07/2013 EN
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Hypoallergenic allergen derivatives of Pru p 3 for immunotherapy of IgE-mediated peach allergy

Linhart, B; Gstoettner, A; Gamez, C; Swoboda, I; Mari, A; Papadopoulos, N; Valenta, R
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 25/07/2013 EN
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Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines

Gómez-Casado, C.; Garrido-Arandia, M.; Gamboa, P.; Blanca-López, N.; Canto, G.; Varela, J.; Cuesta-Herranz, J.; Pacios, L. F.; Díaz-Perales, A.; Tordesillas, L.
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
EN
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Nowadays, treatment of food allergy only considered the avoidance of the specific food. However, the possibility of cross-reactivity makes this practice not very effective. Immunotherapy may exhibit as a good alternative to food allergy treatment. The use of hypoallergenic molecules with reduced IgE binding capacity but with ability to stimulate the immune system is a promising tool which could be developed for immunotherapy. In this study, three mutants of Pru p 3, the principal allergen of peach, were produced based on the described mimotope and T cell epitopes, by changing the specific residues to alanine, named as Pru p 3.01, Pru p 3.02, and Pru p 3.03. Pru p 3.01 showed very similar allergenic activity as the wild type by in vitro assays. However, Pru p 3.02 and Pru p 3.03 presented reduced IgE binding with respect to the native form, by in vitro, ex vivo, and in vivo assays. In addition, Pru p 3.03 had affected the IgG4 binding capacity and presented a random circular dichroism, which was reflected in the nonrecognition by specific antibodies anti-Pru p 3. Nevertheless, both Pru p 3.02 and Pru p 3.03 maintained the binding to IgG1 and their ability to activate T lymphocytes. Thus, Pru p 3.02 and Pru p 3.03 could be good candidates for potential immunotherapy in peach-allergic patients.

LTP cross-reactivity - primary sensitization to mugwort pollen LTP Art v 3, facilitates subsequent sensitisation to peach LTP Pru p 3 in mice

Wangorsch, Andrea; Schülke, Stefan; Gadermaier, Gabriele; Albrecht, Melanie; Wallner, Michael; Randow, Stefanie; Wolfheimer, Sonja; Lidholm, Jonas; Lauer, Iris; Ferreira, Fatima; Toda, Masako; Vieths, Stefan; Reese, Gerald; Scheurer, Stephan
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 17/03/2014 EN
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35.76%

Frequency of LTP (Pru p 3) and Profilin (Pru p 4) sensitisation in 1052 patients referenced to an immunoallergology department in Lisbon

Pereira Santos, M Conceição; Silva, Pedro; Costa, Célia; Pestana, Letícia; Pereira Barbosa, Manuel
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 17/03/2014 EN
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35.96%

Basophils response to Pru p 3 and Ara h 9 in patients sensitised to peach under specific immunotherapy

Gomez, Francisca; Gomez, Enrique; Doña, Inmaculada; Galindo, Luisa; Campo, Paloma; Posadas, Teresa; Torres, Maria Jose; Diaz-Perales, Araceli; Blanca, Miguel; Mayorga, Lina
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 17/03/2014 EN
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35.72%

Development of a hypoallergenic and immunogenic Pru p 3 proline variant for treatment of peach allergy

Eichhorn, Stephanie; Steiner, Markus; Laimer, Josef; Lackner, Peter; Zuidmeer-Jongejan, Laurian; Briza, Peter; Mari, Adriano; van Ree, Ronald; Ferreira, Fatima; Gadermaier, Gabriele
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 17/03/2014 EN
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Poster 1020: Efficacy and safety of sublingual specific immunotherapy with Pru p 3 in a portuguese population - clinical and immunological evaluation during 12 months

Costa, Ana Célia; Duarte, Fátima; Pedro, Elisa; Melo, Alcinda; Barbosa, Manuel Pereira; Santos, Conceição
Fonte: World Allergy Organization Publicador: World Allergy Organization
Tipo: Artigo de Revista Científica
Publicado em 03/02/2014 EN
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Evaluation of peach tolerance after one year of sublingual immunotherapy with LTP (Pru p 3) in allergic patients sensitises to food by LTPs

Bagos, Gador; Gómez, Francisca; Salas, María; Campo, Paloma; Galindo, Luisa; Torres, María Jose; Mayorga, Cristobalina; Jimeno, Lucía; Blanca, Miguel
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 30/03/2015 EN
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Immunological changes after one year of specific immunotherapy with Pru p 3

Gomez, Francisca; Torres, Maria Jose; Bogas, Gador; Galindo, Luisa; Fernandez, Tahía; Melende, Lidia; Barber, Domingo; Blanca, Miguel; Mayorga, Cristobalina
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 30/03/2015 EN
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Prostaglandin F2alpha- and FAS-activating antibody-induced regression of the corpus luteum involves caspase-8 and is defective in caspase-3 deficient mice

Carambula, Silvia F; Pru, James K; Matikainen, Tiina; Gonçalves, Paulo Bayard D; Flavell, Richard A; Lynch, Maureen Patricia; Tilly, Jonathan Lee; Rueda, Bo Ruben
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
EN_US
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We recently demonstrated that caspase-3 is important for apoptosis during spontaneous involution of the corpus luteum (CL). These studies tested if prostaglandin F[sub]2α (PGF[sub]2α) or FAS regulated luteal regression, utilize a caspase-3 dependent pathway to execute luteal cell apoptosis, and if the two receptors work via independent or potentially shared intracellular signaling components/pathways to activate caspase-3. Wild-type (WT) or caspase-3 deficient female mice, 25–26 days old, were given 10 IU equine chorionic gonadotropin (eCG) intraperitoneally (IP) followed by 10 IU human chorionic gonadotropin (hCG) IP 46 h later to synchronize ovulation. The animals were then injected with IgG (2 micrograms, i.v.), the FAS-activating antibody Jo2 (2 micrograms, i.v.), or PGF[sub]2α (10 micrograms, i.p.) at 24 or 48 h post-ovulation. Ovaries from each group were collected 8 h later for assessment of active caspase-3 enzyme and apoptosis (measured by the TUNEL assay) in the CL. Regardless of genotype or treatment, CL in ovaries collected from mice injected 24 h after ovulation showed no evidence of active caspase-3 or apoptosis. However, PGF[sub]2α or Jo2 at 48 h post-ovulation and collected 8 h later induced caspase-3 activation in 13.2 ± 1.8% and 13.7 ± 2.2 % of the cells...

Estudio observacional para la determinación de los perfiles de sensibilización alergénica a pólenes en el área sanitaria de Toledo: establecimiento del grado de concordancia entre diferentes técnicas diagnósticas y papel de los panalérgenos en la interpretación diagnóstica de la alergia a pólenes

Abengózar Muela, Ricardo
Fonte: Universidade de Alcalá Publicador: Universidade de Alcalá
Tipo: info:eu-repo/semantics/doctoralThesis; info:eu-repo/semantics/acceptedVersion Formato: application/pdf
SPA
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También disponible en CD-ROM e Internet; Durante las últimas décadas se ha producido un incremento importante de la enfermedad alérgica respiratoria. El diagnóstico tradicional de la alergia a pólenes se realiza mediante pruebas cutáneas y la determinación de IgE específica a alérgenos completos. Recientemente disponemos de una nueva tecnología sanitaria para realizar el diagnóstico de alergia a pólenes. Se trata del diagnóstico molecular o por componentes. Con este método se puede identificar las positividades que se deben a reactividad cruzada por la participación de panalérgenos, que pueden actuar como factores de confusión en el diagnóstico de pacientes polisensibilizados. Dichas polisensibilizaciones son más frecuentes en climas secos y continentales como el de Toledo. El objetivo principal del proyecto ha sido establecer el mapa de sensibilización a pólenes en el área sanitaria de Toledo mediante el uso de técnicas de diagnóstico molecular, estableciendo al mismo tiempo el grado de concordancia de estas técnicas diagnósticas con las pruebas cutáneas convencionales y, valorando el papel de los panalérgenos como posibles factores que enmascaran el diagnóstico. Como objetivos secundarios nos hemos planteado conocer a qué alérgeno de pólenes se asocia la sensibilización a panalérgenos...

Sensitization Prevalence, Antibody Cross-Reactivity and Immunogenic Peptide Profile of Api g 2, the Non-Specific Lipid Transfer Protein 1 of Celery

GADERMAIER, Gabriele; HAUSER, Michael; EGGER, Matthias; FERRARA, Rosetta; BRIZA, Peter; SANTOS, Keity Souza; ZENNARO, Danila; GIRBL, Tamara; ZUIDMEER-JONGEJAN, Laurian; MARI, Adriano; FERREIRA, Fatima
Fonte: PUBLIC LIBRARY SCIENCE Publicador: PUBLIC LIBRARY SCIENCE
Tipo: Artigo de Revista Científica
ENG
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Background: Celery (Apium graveolens) represents a relevant allergen source that can elicit severe reactions in the adult population. To investigate the sensitization prevalence and cross-reactivity of Api g 2 from celery stalks in a Mediterranean population and in a mouse model. Methodology: 786 non-randomized subjects from Italy were screened for IgE reactivity to rApi g 2, rArt v 3 (mugwort pollen LTP) and nPru p 3 (peach LTP) using an allergen microarray. Clinical data of 32 selected patients with reactivity to LTP under investigation were evaluated. Specific IgE titers and cross-inhibitions were performed in ELISA and allergen microarray. Balb/c mice were immunized with purified LTPs; IgG titers were determined in ELISA and mediator release was examined using RBL-2H3 cells. Simulated endolysosomal digestion was performed using microsomes obtained from human DCs. Results: IgE testing showed a sensitization prevalence of 25.6% to Api g 2, 18.6% to Art v 3, and 28.6% to Pru p 3 and frequent co-sensitization and correlating IgE-reactivity was observed. 10/32 patients suffering from LTP-related allergy reported symptoms upon consumption of celery stalks which mainly presented as OAS. Considerable IgE cross-reactivity was observed between Api g 2...

Prostaglandin F2alpha- and FAS-activating antibody-induced regression of the corpus luteum involves caspase-8 and is defective in caspase-3 deficient mice

Carambula, Silvia F; Pru, James K; Lynch, Maureen P; Matikainen, Tiina; Gonçalves, Paulo Bayard D; Flavell, Richard A; Tilly, Jonathan L; Rueda, Bo R
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em 11/02/2003 EN
Relevância na Pesquisa
35.86%
We recently demonstrated that caspase-3 is important for apoptosis during spontaneous involution of the corpus luteum (CL). These studies tested if prostaglandin F2α (PGF2α) or FAS regulated luteal regression, utilize a caspase-3 dependent pathway to execute luteal cell apoptosis, and if the two receptors work via independent or potentially shared intracellular signaling components/pathways to activate caspase-3. Wild-type (WT) or caspase-3 deficient female mice, 25–26 days old, were given 10 IU equine chorionic gonadotropin (eCG) intraperitoneally (IP) followed by 10 IU human chorionic gonadotropin (hCG) IP 46 h later to synchronize ovulation. The animals were then injected with IgG (2 micrograms, i.v.), the FAS-activating antibody Jo2 (2 micrograms, i.v.), or PGF2α (10 micrograms, i.p.) at 24 or 48 h post-ovulation. Ovaries from each group were collected 8 h later for assessment of active caspase-3 enzyme and apoptosis (measured by the TUNEL assay) in the CL. Regardless of genotype or treatment, CL in ovaries collected from mice injected 24 h after ovulation showed no evidence of active caspase-3 or apoptosis. However, PGF2α or Jo2 at 48 h post-ovulation and collected 8 h later induced caspase-3 activation in 13.2 ± 1.8% and 13.7 ± 2.2 % of the cells...

Eficácia e tolerância de Imunoterapia sublingual com Pru p 3 em doentes com alergia grave ao pêssego: evolução clínica e imunológica ao longo de 12 meses

Costa,Ana Célia; Melo,Alcinda; Duarte,Fátima; Pereira-Barbosa,Manuel; Santos,M Conceição Pereira
Fonte: Sociedade Portuguesa de Alergologia e Imunologia Clínica Publicador: Sociedade Portuguesa de Alergologia e Imunologia Clínica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2015 PT
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Introdução: A alergia ao pêssego é prevalente, persistente e potencialmente grave, sendo as proteínas de transferência lipídica (LTPs) - Pru p 3 e as profilinas - Pru p 4, os principais alergénios envolvidos.A presença oculta de Pru p 3 em produtos alimentares pode desencadear reacções acidentais graves, incluindo anafilaxia e os doentes podem reagir a outros alimentos que contêm LTPs (síndrome LTP). Este tipo de alergia pode ser considerado um alvo importante para imunoterapia (IT) a alergénios. Objectivo: Demonstrar a eficácia e segurança da IT sublingual (SLIT) com Pru p 3 (SLIT-Pru p 3) em doentes com reacções sistémicas (RS) associadas à ingestão de pêssego, através da avaliação de parâmetros clínicos e imunológicos, ao longo de 12 meses. Material e métodos: Oito doentes (7F,1M; média de idades -25,6;19-41 anos) com história de alergia ao pêssego, confirmada por Prova de Provocação Oral (excepto se anafilaxia e evidência de sensibilização) submetidos a SLIT -Pru p 3 (Alk -Abelló) durante 1 ano. Todos os doentes tiveram RS (75% anafilaxia) associadas à ingestão de pêssego (62,5% referiam sintomas com outros alimentos com LTP). Realizaram testes cutâneos por picada (TCP) com bateria de aeroalergénios...