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Phenolic profile of hazelnut (Corylus avellana L.) leaves cultivars grown in Portugal

Amaral, J.S.; Ferreres, F.; Andrade, P.B.; Valentão, P.; Pinheiro, C.; Santos, Alberto; Seabra, R.M.
Fonte: Taylor & Francis Publicador: Taylor & Francis
Tipo: Artigo de Revista Científica
ENG; ENG
Relevância na Pesquisa
56.35%
In this study, phenolic compounds of hazelnut leaves of 10 different cultivars with the same cultural, geographical, geological and climatic conditions were analyzed by HPLC/DAD and HPLC/DAD/MS/MS – ESI. Eight phenolic compounds (3-caffeoylquinic acid, 5-caffeoylquinic acid, caffeoyltartaric acid, p-coumaroyltartaric acid, myricetin 3-rhamnoside, quercetin 3-glycoside, quercetin 3-rhamnoside and kaempferol 3-rhamnoside) were identified and quantified. All of the analyzed samples showed a similar phenolic profile, in which myricetin 3-rhamnoside and quercetin 3-rhamnoside were the major compounds and caffeoyltartaric and p-coumaroyltartaric acids were present in vestigial amounts.

Phenolic profile of Cydonia oblonga Miller leaves

Oliveira, Andreia P.; Pereira, J.A.; Andrade, P.B.; Valentão, P.; Seabra, R.M.; Silva, B.M.
Fonte: ACS Publicador: ACS
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.38%
Cydonia oblonga Miller leaves phenolic compounds were analyzed by reversed-phase HPLC/DAD and HPLC/UV. Qualitative and quantitative analysis of phenolics were carried out in a total of 36 samples of quince leaves from three different geographical origins of Northern (Bragança and Carrazeda de Ansiães) and Central Portugal (Covilhã) and three collection months (June, August, and October of 2006). These leaves presented a common phenolic profile composed by nine compounds: 3-O-, 4-O- and 5-O-caffeoylquinic acids, 3,5-O-dicaffeoylquinic acid, quercetin-3-Ogalactoside, quercetin-3-O-rutinoside, kaempferol-3-O-glycoside, kaempferol-3-O-glucoside, and kaempferol-3-O-rutinoside. 5-O-caffeoylquinic acid was the major phenolic compound (36.2%), followed by quercetin 3-O-rutinoside (21.1%). Quince leaves are characterized by higher relative contents of kaempferol derivatives than fruits (pulps, peels, and seeds), especially in what concerns kaempferol-3-O-rutinoside (12.5%). C. oblonga leaves total phenolic content was very high, varying from 4.9 to 16.5 g/kg dry matter (mean value of 10.3 g/kg dry matter), indicating that these leaves can be used as a good and cheap source of bioactive constituents. Significantly differences were observed in 3-O-caffeoylquinic and 3...

Antioxidant properties and phenolic profile of the most widely appreciated cultivated mushrooms: a comparative study between in vivo and in vitro samples.

Reis, Filipa S.; Barros, Lillian; Martins, Anabela; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.35%
The present study reports a comparison of the antioxidant properties and phenolic profile of the most consumed species as fresh cultivated mushrooms and their mycelia produced in vitro: Agaricus bisporus (white and brown), Pleurotus ostreatus (oyster), Pleurotus eryngii (king oyster) and Lentinula edodes (shiitake). The antioxidant activity was evaluated through reducing power (Folin-Ciocalteu and Ferricyanide/Prussian blue assays), free radical scavenging activity (DPPH assay) and lipid peroxidation inhibition (β-carotene/linoleate and TBARS assays). The analysis of phenolic compounds was performed by HPLC/PAD. The mushroom species with the highest antioxidant potential was Agaricus bispous (brown). However, concerning to the species obtained in vitro, it was Lentinula edodes that demonstrate the highest reducing power. Generally, in vivo samples revealed higher antioxidant properties than their mycelia obtained by in vitro techniques. About the phenolic compounds researched, they were detected both in mushrooms and mycelia without any particular abundance. Results showed that there is no correlation between the studied commercial mushrooms and the corresponding mycelia obtained in vitro. Nevertheless, this study contributes to the rise of data relatively to the species consumed as fresh mushrooms and the possibility of their in vitro production as a source of bioactive compounds.

Phenolic profile of wild fruits of Rosa canina SL. from Northeast Portugal

Guimarães, Rafaela; Barros, Lillian; Reis, Filipa S.; Dueñas, Montserrat; Carvalho, Ana Maria; Santos-Buelga, Celestino; Queiroz, Maria João R.P.; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
46.42%
Plant polyphenols are a wide group of secondary metabolites and are a common component of our diet. Flavonoids represent the most common and widely distributed group of plant phenolics, and can be further broken into classes including flavones, flavonols, flavanones, flavanols, anthocyanins and isoflavonoids. Different classes of bioactive constituents are present in Rosa canina, including phenolic compounds. Rosa canina fruits can be eaten raw as snacks and possess prophylactic and therapeutic activities against a wide range of ailments, including the inflammatory disorders arthritis, rheumatism, gout, colds and gastrointestinal disorders, which might be related with their phenolic composition. This study aimed to characterize the phenolic compounds present in the above mentioned wild fruits. The analysis of phenolic compounds was carried out by reversed-phase HPLC-DAD and the major phenolic compounds were identified by ESI-MS, in order to establish the specific phenolic profile. Rosa canina presented different classes of flavonoids. Flavones, flavonols and dihydroflavonols represented 5.50 mg/100 g dry weight, among which taxifolin, a dihydroflavonol, was prominent (1.18 mg/100 g). Flavan-3-ols (i.e., catechins and proanthocyanidins) were other relevant flavonoids found. (+)-Catechin was the most abundant flavan-3-ol found in the fruits (3.59 mg/100 g)...

Phenolic profile of hydromethanolic extract and decoction of Rubus ulmifolius Schott

Martins, Ana; Barros, Lillian; Dueñas, Montserrat; Carvalho, Ana Maria; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
46.48%
Rubus ulmifolius Schoot (Rosaceae), known as wild blackberry, is a perennial shrubfound in wild and cultivated habitats in Europe, Asia and North Africa[1]. Traditionally, R. ulmifoliusis considered to be anticatarrhal, antiseptic, diuretic, anti-inflammatory, antioxidant, astringent, and antispasmodic. Oral dosage forms are used for diarrhea, menstrual pain, menopause disorders, liver diseases, aphtha, gingivitis, hypertension and diabetes [2].Different classes of bioactive constituents are present in R. ulmifolius, including phenolic compounds. This study aimed to determine the phenolic composition of different preparations (decoction and hydromethanolic extract)of R. ulmifoliusflower buds. The analysis of phenolic compounds was carried out by reversed-phase high performance liquid chromatography coupled to diode array detection and mass spectrometry with electron spray ionization (HPLC-DAD-ESI/MS), in order to establish the specific phenolic profile of each preparation. The phenolic profile of both preparations was identical, varying only in the concentrations found. Twenty-three phenolic compounds were identified in both samples, in which seven were identified as phenolic acids (di- and caffeolyqui nic, p-coumaroylquinicand feruloylquinic acids)...

Phenolic profile of wild Achillea millefolium L. obtained by HPLC-DADESI/MS.

Dias, Maria Inês; Barros, Lillian; Dueñas, Montserrat; Carvalho, Ana Maria; Oliveira, M.B.P.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
46.38%
Achillea millefolium L., commonly known as yarrow, belongs to Asteraceae family and it is very common in mountain meadows, pathways, crop fields and homegardens. It is widely used in Europe as an herbal remedy to treat digestive problems, diabetes, hepato-biliary diseases and amenorrhea, being also consumed for its antitumor, antimicrobial, anti-inflammatory and antioxidant properties [1,2]. Some studies related A. millefolium medicinal properties to the presence of phenolic compounds, namely flavonoids and phenolic acids [3,4]. In the present study, the phenolic profile of wild A. millefolium from the Northeastern Portugal was evaluated by HPLC-DAD-ESI/MS, after extraction with methanol:water (80:20, v:v). Twenty eight different compounds were identified: ten phenolic acids, in which 5-O-caffeolyquinic and 3,5-O-dicaffeolyquinic acids were the main compounds; eight flavones, namely different glycosylates forms of apigenin, being apigenin acylhexoside the major flavones; and ten flavonols, with quercetin derivatives as main compounds. Overall, this study reveals that A. millefolium is an important source of bioactive molecules, namely phenolic compounds that could be related to its pharmaceutical and ethnobotanical uses.

Phenolic profile of wild fruits of Rosa micrantha ex Sm. and Rosa canina L. from Northeast Portugal

Guimarães, Rafaela; Barros, Lillian; Dueñas, Montserrat; Carvalho, Ana Maria; Queiroz, Maria João R.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
46.48%
Phenolic metabolites are common constituents of fruits and vegetables, and t he interest of plant phenolic extracts derives from the evidence of their potent antioxidant activity and their wide range of pharmacologic properties including anticancer, antimicrobial and platelet aggregation inhibition activities [1]. Rosa canina L. fruits are eaten raw as snacks and possess prophylactic and therapeutic activities against a wide range of ailments. Rosa micrantha ex Sm. fruits are used to prepare homemade remedies that prevent or heal several human disorders and animal diseases [2] This study aimed to characterize the phenolic compounds present in the above mentioned wild fruits. The analysis of phenolic compounds was carried out by reversed-phase HPLC-DAD-ESI/MS, in order to establish the specific phenolic profile in two Rose species. R. canina and R. micrantha presented a very similar profile being detected different classes of flavonoids, but no phenolic acids were found in these samples. The highest flavone/ols (11.16 mg/100g), flavan-3-ols and galloyl derivates (19.90 mg/100g), and anthocyanins (1.19 mg/100g) content were found in R. micrantha fruits. Taxifolin pentoside was the most abundant flavanonol found in these fruits and represented 1.18 mg/100 mg for R. canina and 2.68 mg/100 g for R. micrantha. Methyl gallate hexoside was only found in R. micrantha (2.45 mg/ 100g). (+)-Catechin was the most abundant flavan-3-ol found in the samples (R. canina 3.59 mg/100 g and R. micrantha 4.93 mg/ 100g)...

Antimicrobial activity, phenolic profile and role in the inflammation of propolis

Silva, João; Rodrigues, Sandra; Feás, Xesús; Estevinho, Leticia M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.31%
Nowadays a great amount of information regarding chemical and biological aspects of bee products is available in the literature, but few data on its therapeutic uses are found. The aim of this study was to evaluate the phenolic profile, the in vitro antimicrobial activity and effect in the hyaluronidase enzyme (widely related with the inflammation process) of propolis harvested in Portugal. The efficacy of three extracts (hydro-alcoholic, methanolic and aqueous) was also compared. It was chosen the hydroalcoholic extract, because this was the most effective for extracting phenolic compounds. The antimicrobial activity was accessed in Gram-positive and Gram-negative bacteria and yeasts, isolated from different biological fluids and the results were then compared with the obtained for reference microorganisms. The propolis from Bragança was the one that possessed the highest polyphenols’ content. The sample from Beja showed the less significant inhibition of the hyaluronidase enzyme. Concerning the antimicrobial activity, Candida albicans was the most resistant and Staphylococcus aureus the most sensitive. The reference microorganisms were more sensitive than the ones isolated from biological fluids.

Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.

Živković, Jelena; Barreira, João C.M.; Stojković, Dejan; Cebovic, Tatjana; Santos-Buelga, Celestino; Maksimović, Zoran; Ferreira, Isabel C.F.R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
66.37%
This study was designed to characterize the phenolic profile and evaluate the antibacterial, antimutagenic and antitumor activities of Veronica urticifolia Jacq. methanolic extract. HPLC-DAD/ESI-MS analysis revealed the presence of phenolic acids, flavonoids and phenylethanoids, with acteoside as the main component (14.9 mg/g of extract). Antibacterial effect was determined using the microbroth dilution assay and Staphylococcus aureus was the most sensitive strain (MIC and MBC = 7.5 mg/mL). Antimutagenic activity was evaluated by Ames mutagenicity assay. At 1 mg/plate, the tested extract afforded high protection against the mutagenicity of nitroquinoline-N-oxide (4NQO) to Salmonella typhimurium strain TA100 (inhibition rate 48.3%). Antitumor activity was screened in Ehrlich ascites carcinoma (EAC) model. Pretreatment with 2 mg/kg body weight showed statistically significant decrease in tumor cell viability, while ascites volume and tumor cell count became slightly decreased, but not to a statistically significant extent. Above results indicate that V. urticifolia deserves further research into chemoprevention.

Phenolic profile of Sercial and Tinta Negra Vitis vinifera L. grape skins by HPLC-DAD-ESI-MSn: Novel phenolic compounds in Vitis vinifera L. grape

Perestrelo, Rosa; Lu, Ying; Santos, Sónia A. O.; Silvestre, Armando J. D.; Neto, Carlos P.; Câmara, José S.; Rocha, Sílvia M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.5%
This study represents the first phytochemical research of phenolic components of Sercial and Tinta Negra Vitis vinifera L. The phenolic profiles of Sercial and Tinta Negra V. vinifera L. grape skins (white and red varieties, respectively) were established using high performance liquid chromatography-diode array detection-electrospray ionisation tandem mass spectrometry (HPLC-DAD-ESI-MSn), at different ripening stages (veraison and maturity). A total of 40 phenolic compounds were identified, which included 3 hydroxybenzoic acids, 8 hydroxycinnamic acids, 4 flavanols, 5 flavanones, 8 flavonols, 4 stilbenes, and 8 anthocyanins. For the white variety, in both ripening stages, hydroxycinnamic acids and flavonols were the main phenolic classes, representing about 80% of the phenolic composition. For red variety, at veraison, hydroxycinnamic acids and flavonols were also the predominant classes (71%), but at maturity, anthocyanins represented 84% of the phenolic composition. As far as we know, 10 compounds were reported for the first time in V. vinifera L. grapes, namely protocatechuic acid-glucoside, p-hydroxybenzoyl glucoside, caftaric acid vanilloyl pentoside, p-coumaric acid-erythroside, naringenin hexose derivate, eriodictyol-glucoside...

Multivariate statistical approaches for wine classification based on low molecular weight phenolic compounds

Cabrita, Maria Joao; Aires de Sousa, Joao; Gomes da Silva, Marco; Rei, Fernando; Costa Freitas, Ana Maria
Fonte: Australian Society of Viticulture and Oenology Publicador: Australian Society of Viticulture and Oenology
Tipo: Artigo de Revista Científica
POR
Relevância na Pesquisa
46.55%
Background and Aims: Phenolic compounds influence the colour, flavour and astringency of wines. These compounds are extracted into the wine during grape fermentation and maceration and thus the winemaking process is the main factor affecting the phenolic content of wines, besides the varietal factor. In this work, we aimed to apply self organizing maps to investigate the relationships between the profile of phenolic compounds and grape variety of wines, as well as the changes in the phenolic profile resulting from the malolactic fermentation. The results are compared with principal component analysis, and variation partitioning. Methods and Results: A reversed phase liquid chromatography/DAD method was used for the analysis of major non-flavonoid phenolic compounds in wines. The method employed allowed to evaluate the impact of malolactic fermentation in low molecular phenolic compounds in different wine varieties: Trincadeira, Aragonez, Cabernet Sauvignon, Alfrocheiro, Castelão and Touriga Nacional. The malolactic fermentation process was also study in Trincadeira variety using indigenous bacteria and two different commercial lactic bacteria. The impact of malolactic fermentation and grape varieties on the phenolic profile was evaluated by different multivariate statistical approaches: principal component analysis...

Phenolic composition and antioxidant activity of Rocha pear and other pear cultivars: a comparative study

Salta, J.; Martins, A.; Santos, R. G.; Neng, N. R.; Nogueira, J. M.; Justino, Jorge; Rauter, A. P.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em //2010 ENG
Relevância na Pesquisa
56.31%
The phenolic profile and the antioxidant activity of Rocha pear, a Portuguese pear cultivar, were determined and compared with the commercially available pear varieties Cornice, Abate, General Leclerc and Passe Crassane. Phenolic composition of the methanolic extracts of these pears was determined by high performance liquid chromatography with diode array detection (HPLC-DAD), while antioxidant activities were evaluated using three complementary test systems: DPPH radical scavenging activity, ferric reducing power capacity and beta-carotene/linoleic acid bleaching assay. When compared to the studied varieties, Rocha pear (peel and flesh) presented the highest content of total phenolics. Among them, chlorogenic, syringic, ferulic and coumaric acids, arbutin and (-)-epicatechin were detected as major components. In addition, among the tested varieties, Rocha pear presented the best antioxidant activities in the DPPH and ferric reducing power assays.

Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic profile, antioxidant activity and prion binding properties

Justino, Jorge; Rauter, Amélia; Dias, Catarina; Martins, Alice; Branco, Isabel; Goulart, Margarida; Nogueira, José; Neng, Nuno; Silva, Filipa; Trevitt, Clare; Waltho, Jon
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /06/2012 POR
Relevância na Pesquisa
56.37%
Estudo de produção agronómica de espécis da salvia com vista à avaliação dos seus constituintes para o potencial controlo da doença de Alzaimer; Salvia sclareoides is an aromatic herb native to Portugal, of which phenolic content (Folin–Ciocalteau method), chemical profile (HPLC/DAD), antioxidant activity (DPPH, β-carotene/linoleic acid assays), acute toxicity (MTT method, adapted for non-adherent cells), genotoxicity (short-term chromosomal aberration assay) and prion binding properties were evaluated in the acetone, water, ethanol, methanol and n-butanol extracts. The latter presented the highest phenolic content and antioxidant activity (DPPH assay), and was the single one with the flavonoids (+)-catechin, kaempferol O-glucoside and quercetin. Vanillic acid was the major component of all extracts but gallic, gentisic, caffeic, syringic, coumaric and ferulic acids were also found in some extracts. Only the n-butanol extract had components binding to the cellular form of human prion protein detected by NMR which showed specificity for two regions of the folded domain and for the unstructured N-terminal region. Extracts were not cytotoxic nor genotoxic, reinforcing the potential of S. sclareoides for nutraceutical purposes.

Simultaneous analysis of 25 phenolic compounds in grape juice for HPLC: method validation and characterization of São Francisco Valley samples

Natividade, M. M. P.; Corrêa, L. C.; Souza, S. V. C.; Pereira, G. E.; Lima, L. C. O.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em 15/08/2013 EN
Relevância na Pesquisa
56.43%
A HPLC method for simultaneous determination of 25 phenolic compounds in grape juice was optimized, validated and applied in the characterization of juices produced in São Francisco Valley (SFV), Brazil. The performance char- acteristics of the method were established by assays with standard solutions of phenolic compounds, spiked and unspiked samples. Linearity, matrix effects, trueness, precision, detection and quantification limits were evaluated. Linearity was demonstrated in the concentration ranges tested for all phenolic compounds. Significant matrix ef- fects were not identified for the studied compounds. Mean recoveries ranged from 86.18 to 106.50%, demonstrat- ing no lack of trueness. Precision of the method was confirmed for the 25 phenolic compounds, with acceptable repeatability relative standard deviations (from 0.71 to 9.24%) and within-reproducibility relative standard devia- tions (from 1.34 to 9.26%) for unspiked and spiked samples. The theoretical limits of detection and quantification of the method varied from 0.001 to 0.19 μg mL −1 and 0.003 to 0.37 μg mL −1 , respectively. The results of the valida- tion process showed that the proposed method is fitness for purpose. This method was able to identify simulta- neously 25 phenolic compounds and had advantages such as low consumption of solvents and easy sample preparation. The phenolic profile of the grape juices from SFV varied according the grape cultivar. Phenolics of the anthocyanins and tannins class predominated in red grape juices...

Antioxidant activity and phenolic profile of commercial and wild roots of Fragaria vesca L.

Dias, Maria Inês; Barros, Lillian; Oliveira, M.B.P.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
46.41%
Fragaria vesca L (Rosaceae), wild strawberry, is commonly found in forests, slopes and roadsides. Widely spread across Europe, it can be also found in Korea, Japan, North America and Canada [1 ]. The roots are traditionally used as decoction and infusion to treat cough symptoms, urinary tract infections, haemorrhoids, diarrhoea, and gout, but also for their tonic, stimulant and diuretic properties. Those bioactive properties have been mainly related with the composition in various phenolic compounds such as anthocyanins, proanthocyanidins, flavonols, and derivatives of hydroxycinnamic and ellagic acids [2, 3]. As far as we know, there are no reports on the phenolic profile and antioxidant activity of F. vesca roots. In the present study, commercial and wild roots of this species were submitted to different extraction procedures, aqueous (by infusion or decoction) and hydromethanolic, in order to compare their antioxidant potential and to establish the individual phenolic profile of each one. Commercial and wild samples showed similarities in terms of flavan 3-ols (TF30), with catechin derivatives, mainly procyanidins, as major compounds in both samples. The commercial sample presented ellagic acid glycosides, whereas the wild sample presented flavonols (TF) and dihydroflavonols (taxifolin derivatives). The infusion of wild sample gave the highest content of total phenolic compounds (TPC...

Phenolic profile and antioxidant properties of commercial and wild Fragaria vesca L. roots: A comparison between hydromethanolic and aqueous extracts

Dias, Maria Inês; Barros, Lillian; Oliveira, M. Beatriz P.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.31%
The phenolic profile of hydromethanolic extracts, infusions and decoctions of commercial and wild samples of Fragaria vesca (wild strawberry) roots was obtained by HPLC-DAD/ESI-MS, and further correlated with their antioxidant properties. Commercial and wild samples showed similarities in terms of flavan-3-ols (TF3O), with catechin derivatives, mainly procyanidins, as major compounds in both samples. The commercial sample presented ellagic acid glycosides, whereas the wild sample presented flavonols (TF) and dihydroflavonols (TdhF, taxifolin derivatives). The infusion of wild sample gave the highest content of total phenolic compounds (TPC), DPPH (2,2-Diphenyl-1-picrylhydrazyl) scavenging activity, reducing power and TBARS (thiobarbituric acid reactive substances) inhibition. The antioxidant capacity (mainly β-carotene bleaching and TBARS inhibition) observed for the wild sample is correlated with TF3O, TF and TPC. Overall, the high antioxidant potential of F. vesca roots was demonstrated and could be achieved directly by consumption of infusions/decoctions or by incorporating hydromethanolic extracts in antioxidant formulations.; The authors are grateful to Fundação para a Ciência e a Tecnologia (FCT, Portugal) for financial support to CIMO (strategic project PEst-OE/AGR/UI0690/2011) and REQIMTE (PEst-C/EQB/LA0006/2011)...

Comparative study of the phenolic profile and antioxidant properties of Chamaemelum nobile: infusion, decoction, and hydroalcoholic extract

Guimarães, Rafaela; Barros, Lillian; Dueñas, Montserrat; Carvalho, Ana Maria; Santos-Buelga, Celestino; Queiroz, Maria João R.P.; Ferreira, Isabel C.F.R.
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
46.38%
This stud} aimed to determine the phenolic composition and to evaluate the antioxidant activity o f different preparation' of Roman chamomile: infusion. decoction and methanol: water (80:20) extract. The hydroalcoholic extract revealed the highest antioxidant activity in almost all the performed assays (EC\ , ≤0.62 mg/ml. depending on the assay ). which was in agreement with its highest total content in phenolic compounds. Otherwise. decoctions presented the lowest antioxidant potential (EC50 ≤1.4S mg/mL. depending on the assay). The phenolic profile of the different preparations was identical. varying only in the concentrations found. Phenolic acids (caffooylquinic acids). flavonols (quercetin and kaempferol derivatives) and flavones (apigenin and luteolin derivatives) were found in the three preparations. The most abundant compounds in the infusion and hydroalcoholic ex tract we re 5-0 -caffeoylquinic acid and an apigenin derivative. These. as also the other phenolic compounds, decreased significantly in the decoction .

Phenolic profile and antioxidant potential of wild watercress (Nasturtium officinale L.)

Zeb, Alam
Fonte: Springer International Publishing Publicador: Springer International Publishing
Tipo: Artigo de Revista Científica
Publicado em 24/11/2015 EN
Relevância na Pesquisa
46.48%
Phenolic profile, antioxidant potential and pigment contents of wild watercress (Nasturtium officinale L.) were studied to assess the potential for future studies and its applications in neutraceuticals and bioactive functional ingredients. Different extracts of watercress (roots, stem and leaves) were analysed for pigment composition, total phenolic contents, and radical scavenging activity. The phenolic profile of the leaves and roots was studied using reversed phase HPLC–DAD. Results showed that total phenolic compounds in all samples were higher in the methanolic extracts than its corresponding aqueous extracts. The RSA of methanolic extracts was higher than aqueous extracts. Fourteen phenolic compounds were identified in the leaves, where coumaric acid and its derivatives, caftaric acid and quercetin derivatives were present in higher amounts. In roots, a total of 20 compounds was tentatively identified, with coumaric acid and its derivatives, sinapic acid, caftaric acid and quercetin derivatives were the major phenolic compounds. In conclusion, watercress has significant antioxidant activity and contains important phenolic compounds, which could be of potential biological interest.

Phenolic profile of Sercial and Tinta Negra Vitis vinifera L. grape skins by HPLC–DAD–ESI-MSn: novel phenolic compounds in Vitis vinifera L. grape

Perestrelo, Rosa; Lu, Ying; Santos, Sónia A. O.; Silvestre, Armando J. D.; Neto, Carlos P.; Câmara, José S.; Rocha, Sílvia M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /11/2012 ENG
Relevância na Pesquisa
56.5%
This study represents the first phytochemical research of phenolic components of Sercial and Tinta Negra Vitis vinifera L. The phenolic profiles of Sercial and Tinta Negra V. vinifera L. grape skins (white and red varieties, respectively) were established using high performance liquid chromatography–diode array detection–electrospray ionisation tandem mass spectrometry (HPLC–DAD–ESI-MSn), at different ripening stages (véraison and maturity). A total of 40 phenolic compounds were identified, which included 3 hydroxybenzoic acids, 8 hydroxycinnamic acids, 4 flavanols, 5 flavanones, 8 flavonols, 4 stilbenes, and 8 anthocyanins. For the white variety, in both ripening stages, hydroxycinnamic acids and flavonols were the main phenolic classes, representing about 80% of the phenolic composition. For red variety, at véraison, hydroxycinnamic acids and flavonols were also the predominant classes (71%), but at maturity, anthocyanins represented 84% of the phenolic composition. As far as we know, 10 compounds were reported for the first time in V. vinifera L. grapes, namely protocatechuic acid-glucoside, p-hydroxybenzoyl glucoside, caftaric acid vanilloyl pentoside, p-coumaric acid-erythroside, naringenin hexose derivate, eriodictyol-glucoside...

Phenolic profile of hazelnut ( Corylus Avellana L.) leaves cultivars grown in Portugal

Amaral, Joana S.; Ferreres, Federico; Andrade, Paula B.; Valentão, Patricia; Pinheiro, Cristina; Santos, Alberto; Seabra, Rosa M.
Fonte: Taylor & Francis Publicador: Taylor & Francis
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
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56.35%
7 pages, 1 table, 1 figure.; In this study, phenolic compounds of hazelnut leaves of 10 different cultivars with the same cultural, geographical, geological and climatic conditions were analyzed by HPLC/DAD and HPLC/DAD/MS/MS - ESI. Eight phenolic compounds (3-caffeoylquinic acid, 5-caffeoylquinic acid, caffeoyltartaric acid, p -coumaroyltartaric acid, myricetin 3-rhamnoside, quercetin 3-glycoside, quercetin 3-rhamnoside and kaempferol 3-rhamnoside) were identified and quantified. All of the analyzed samples showed a similar phenolic profile, in which myricetin 3-rhamnoside and quercetin 3-rhamnoside were the major compounds and caffeoyltartaric and p -coumaroyltartaric acids were present in vestigial amounts.; J.S. Amaral is grateful to PRODEP III for financial support.; Peer reviewed