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Alkaline sulfite/anthraquinone pretreatment followed by disk refining of Pinus radiata and Pinus caribaea wood chips for biochemical ethanol production

Franco, Heriberto; Ferraz, André Luís; Milagres, Adriane Maria Ferreira; Carvalho, Walter de; Freer, Juanita; Baeza, Jaime; Teixeira Mendonca, Regis
Fonte: WILEY-BLACKWELL; MALDEN Publicador: WILEY-BLACKWELL; MALDEN
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
67.15%
BACKGROUND: Alkaline sulfite/anthraquinone (ASA) cooking of Pinus radiata and Pinus caribaea wood chips followed by disk refining was used as a pretreatment for the production of low lignified and high fibrillated pulps. The pulps produced with different delignification degrees and refined at different energy inputs (250, 750 and 1600 Wh) were saccharified with cellulases and fermented to ethanol with Saccharomyces cerevisiae using separated hydrolysis and fermentation (SHF) or semi-simultaneous saccharification and fermentation (SSSF) processes. RESULTS: Delignification of ASA pulps was between 25% and 50%, with low glucans losses. Pulp yield was from 70 to 78% for pulps of P. radiata and 60% for the pulp of P. caribaea. Pulps obtained after refining were evaluated in assays of enzymatic hydrolysis. Glucans-to-glucose conversion varied from 20 to 70%, depending on the degree of delignification and fibrillation of the pulps. The best ASA pulp of P. radiata was used in SHF and SSSF experiments of ethanol production. Such experiments produced maximum ethanol concentration of 20 g L-1, which represented roughly90% of glucose conversion and an estimated amount of 260 L ethanol ton(-1) wood. P. caribaea pulp also presented good performance in the enzymatic hydrolysis and fermentation but...

Apreciación cuantitativa del color de la madera por colorimetría. Parte 2 : de los cambios de color en pino radiata (pinus radiata) secado bajo un programa convencional acelerado; Aesthetics appreciation of wood colour and patterns by colorimetry : part 2. Measurements of kiln drying discoloration in radiata pine

Ananías, Rubén A.; Sarzosa, Rodrigo; Janin, Gérard; Bluskova, Genka; Gonçalez, Joaquim Carlos
Fonte: Universidade de Brasília Publicador: Universidade de Brasília
Tipo: Artigo de Revista Científica
ESP
Relevância na Pesquisa
67.17%
En la primera parte de este trabajo se presentaron las bases teóricas en las que se sustenta la medición cuantitativa y objetiva del color de un material. En base al sistema CIELab, se cuantifican los cambios de color de la madera de pino radiata Pinus radiata observados durante el secado industrial bajo un programa convencional acelerado. Los resultados confirman que las mediciones cuantitativas del color en la superficie de la madera permiten detectar la presencia de los cambios de color productos del secado por debajo de la superficie hasta una profundidad de 3 mm. Tales cambios de color son influenciados por la heterogeneidad de los anillos de crecimiento: relación madera inicial/madera final y el ancho de anillos. Además permiten adaptar el programa de secado de acuerdo al nivel de intensidad de la coloración permitida. _______________________________________________________________________________ ABSTRACT; On the first part of this work the colorimetry theory for the CIELAB system was presented. In this paper the drying discoloration of radiata pine Pinus radiata at accelerated temperature was measured quantitatively and objectively. The results showed that the kiln brown drying would be detected until 3 mm below the surface by colorimetry and that this parameters values were influenced by the heterogeneity of the annual ring: earlywood/latewood relation and annual ring width. Also this measurements of the wood colour changes can allow to adjust the drying schedule in according to the coloration intensity allowed.

Tree growth in a silvopastoral system established in acid soils with Pinus radiata D. Don

Mosquera-Losada, R.; Rigueiro-Rodriguez, A.; Ferreiro-Dominguez, N.
Fonte: EURAF Publicador: EURAF
Tipo: Outros
Publicado em /06/2014 ENG
Relevância na Pesquisa
66.88%
Poster; In Galicia (northwestern Spain), the soils tend to be acidic mainly due to the region’s humid climate, the prevalence of subtractive systems, frequent fires and, often, acidic parent material which can limit the tree growth and the pasture production in the silvopastoral systems. Therefore, it is advisable to carry out management techniques and fertilisation to improve soil fertility as well as the pasture and tree productivity. The objective of this experiment was to compare the effect of no fertilisation, three doses of sewage sludge (160, 320 and 480 kg N total ha-1), with or without liming (2.5 t CaCO3 ha–1), and the mineral fertilisation usually used in the region (8% N – 24% P2O5 – 16% K2O) on tree growth in a silvopastoral system established on an acidic forest soil with Pinus radiata D. Don in 1997. In the first years of the study, the results showed an increase of tree growth in the limed plots probably due to the reduction in the Al saturation percentage in the soil exchange complex. However, 12 years after the addition of lime and 9 years after the fertilisation with sewage sludge, the Al saturation percentage in the soil exchange complex was similar to that observed at the beginning of the study which decreased the tree growth in all plots and significant differences between treatments were not observed. Therefore...

Calibration of the parameters of Yield-SAFE model in silvopastoral systems under Pinus radiata D. Don

Ferreiro-Dominguez, N.; Palma, J.H.N.; Rigueiro-Rodriguez, A.; Minnuno, F.; Mosquera-Losada, R.
Fonte: EURAF Publicador: EURAF
Tipo: Outros
Publicado em /06/2014 ENG
Relevância na Pesquisa
67.12%
Poster; The establishment of silvopastoral systems in which trees, animals and pasture are integrated within the same area is promoted by the EU (Council Regulation 1698/2005 and 1305/2013) because these systems diversify and sustain production with increased social, economic and environmental benefits for land users at several levels. However, the environmental and economic benefits of silvopastoral systems are highly difficult to predict due to the interaction of many factors. Furthermore research through field experiments is expensive and time-consuming when tree measurements have to be taken into account. One option to determine the benefits of silvopastoral systems could be the use of Yield-SAFE model that provides one method for overcoming these drawbacks. Moreover, in Galicia (NW Spain) the environmental and economic benefits of silvopastoral systems are different from the rest of Europe mainly due to the region’s humid climate and they have not yet been adequately evaluated. The objective of this study was the calibration of the parameters of the Yield-SAFE model in silvopastoral systems established with Pinus radiata D. Don in Galicia (NW Spain). Parameter calibration of Yield-SAFE model was performed after measuring height and diameter of Pinus radiata D. Don established at two densities (833 y 2500 trees ha-1) from 1996 to 2013. Moreover...

Extração e caracterização de hemiceluloses de Pinus radiata e sua viabilidade para a produção de bioetanol

Reyes,Pablo; Mendonça,Regis Teixeira; Aguayo,María Graciela; Rodríguez,Jaime; Vega,Beatriz; Fardim,Pedro
Fonte: Sociedade de Investigações Florestais Publicador: Sociedade de Investigações Florestais
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2013 PT
Relevância na Pesquisa
66.96%
As galactoglucomananas são as principais frações de hemiceluloses presentes nas madeiras moles e contêm, principalmente, as hexoses galactose, glicose e manose. O isolamento eficiente e seletivo dessas hemiceluloses é um obstáculo crítico a superar para sua utilização. Os objetivos deste trabalho foram extrair e caracterizar soluções aquosas ácidas e neutras de hemiceluloses de cavacos de madeira de Pinus radiata, bem como avaliar sua viabilidade para a produção de bioetanol. As hemiceluloses em P. radiata representam 26 g/100 g de madeira (base seca), e as hexoses são responsáveis por aproximadamente 64% dessa quantidade. De acordo com as diferentes condições de extração, cerca de 50% da fração hemicelulósica foi solubilizada e recuperada depois de uma precipitação com etanol. As frações recuperadas de hemiceluloses estavam na forma de oligômeros com peso molecular médio (Mw) variando entre 4x10³ e 4x10(5) g/mol. Os oligômeros hemicelulósicos foram hidrolisados com ácido sulfúrico diluído e os hidrolisados concentrados até aproximadamente 70 g/L hexosas e fermentados pela levedura Saccharomyces cerevisiae. Os resultados de fermentação indicaram que os açúcares obtidos dos extratos ácidos e neutros foram fermentados com rendimentos máximos de etanol de 63% e 54% (22 g/L e 19 g/L)...

VARIATION OF MICROFIBRIL ANGLE OF Pinus radiata D. Don IN RELATION TO TREE SPACING IN CHILEAN PLANTATIONS

Alteyrac,Jerome
Fonte: Sociedade de Investigações Florestais Publicador: Sociedade de Investigações Florestais
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2015 EN
Relevância na Pesquisa
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ABSTRACTFour stands of 28-year-old radiata pine (Pinus radiata D. Don) grown in the eighth region (Biobio) of Chile were sampled to determine the effect of tree spacing on the microfibril angle. The samples were taken at two different stem levels of the tree, 2.5 m and 7.5 m, with increment strip taken in the Nothern direction. The four experimental stands were characterized by the following spacing 2x2, 2x3, 3x4 and 4x4. The microfibril angle was measured by X-ray diffraction with the SilviScan technology at the FP-Innovation-Paprican Division in Vancouver, Canada. The results showed a significant effect of tree spacing on the microfibril angle in both juvenile wood and mature wood as well as at the two stem levels considered. The minimum (9.42º) was reached in 2x2 stand at 7.5 m in mature wood, while maximum microfibril angle (24.54º) was obtained in 2x3 stand at 2.5 m in juvenile wood. Regarding the effect of tree spacing, 4x4 stand had the lowest microfibril angle,except in mature wood at 7.5 m where 4x4 had the highest microfibril angle (11°) of the four stands.

Estimation of aerial biomass using discrete-wave LiDAR data in combination with different vegetation indices in plantations of Pinus radiata (D. DON), Región del Maule, Chile.

Fonte: Universidade Católica de Temuco Publicador: Universidade Católica de Temuco
Tipo: Artículo de Revista
EN
Relevância na Pesquisa
66.88%
The aerial biomass of Pinus radiata plantations in the Región del Maule, Chile, was estimated from linear models using databases of LiDAR and multispectral LANDSAT ETM+. Six descriptive height variables were obtained from the LiDAR point cloud; the 25%, 50%, 75%, 95% and 100% percentiles and the mean height. Two variables associated with the density of points were also obtained, which relate the returns between fixed weighted intervals calculated as a function of the observed biomass. For multispectral variables we used NDVI, corrected NVDI (NDVIc) and the “Tasseled Cap” components brilliance, greenness and humidity. The results showed coefficients of determination (R2) between 0.801 and 0.814, with errors between 36.07 and 36.11 ton ha-1 for the models generated using height percentiles, and R2 from 0.807 to 0.823 with errors between 36.06 and 36.84 ton ha-1 for transformed LiDAR data. Finally, the stepwise model using all available variables had R2 of 0.821-0.835 with errors of 34.28 - 36.31 ton ha-1.; La biomasa aérea en bosques de pino insigne en la región del Maule, Chile, fue estimada utilizando modelos lineales sobre la base de datos LiDAR y multiespectrales de LANDSAT ETM+. De la nube de puntos LiDAR se obtuvo un total de seis variables descriptivas de altura...

Effect of the reaction conditions over the yield and properties of methylcellulose obtained from Pinus radiata kraft bleached cellulose

Basualto Flores, Carlos Alfonso; Valenzuela Lozano, Fernando; Andrade, C. T.; Sapag Hagar, Jaime; Hasson Muchnick, Jaime Mauricio; Tapia Villanueva, Cristián
Fonte: SOCIEDAD CHILENA DE QUIMICA Publicador: SOCIEDAD CHILENA DE QUIMICA
Tipo: Artículo de revista
EN
Relevância na Pesquisa
66.88%
Kraft bleached cellulose from Pinus radiata was submitted to methylation reactions in heterogeneous media. After activation with 29% (w/w) NaOH solution, methylation reactions were carried out both with dimethyl sulfate (DMS) and methyl iodide (MI). The effects of the pressing ratio, reaction time and type of methylation agent were studied. The better yields were obtained with dimethylsulphate and the pressing ratio and reaction time were not signficant over the yield. Methylcellulose (MC) of high degree of substitution was extracted with water and the MC of low degree of substitution was extracted with 8%w/w NaOH. Some of the MCs obtained were characterized by viscosity average molecular weight, thermal behavior, degree of substitution, steady shear solution behavior. The MC water extracted obtained under certain reaction conditions showed similar behavior that commercial MC.

Site index prediction for Pinus radiata D. Don at local scale on the southern tablelands of New South Wales using a national forest productivity model

Ford, Andrew; Brack, Cristopher; James, Ryde
Fonte: Institute of Foresters of Australia Publicador: Institute of Foresters of Australia
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
66.88%
Productivity indices, developed by the Australian Greenhouse Office as part of the National Carbon Accounting System (NCAS) to aid spatial estimation of native forest productivity across Australia, were tested to estimate site quality for Pinus radiata D.

Effects of irrigation of N fertilization on growth and structure of Pinus radiata stands between 10 and 29 years of age

Waterworth, Rob; Raison, R John; Brack, Cristopher; Benson, Martin; Khanna, Partap; Paul, Keryn
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
66.88%
Temporal change in stem volume, basal area, diameter, and height are reported for stands of Pinus radiata growing near Canberra, ACT, Australia between the ages of 10 and 29 years. The stands were subjected to either a single fertilization (F) at age 10 y

Observed and modelled soil carbon and nitrogen changes after planting a Pinus radiata stand onto former pasture

Kirschbaum, Miko; Guo, Lan Bin; Gifford, Roger M
Fonte: Pergamon-Elsevier Ltd Publicador: Pergamon-Elsevier Ltd
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
66.88%
After reforesting pasture land, it is often observed that soil carbon stocks decrease. The present work reports findings from a site near Canberra, Australia, where a pine forest (Pinus radiata) was planted onto a former unimproved pasture site. We report

Fertiliser and irrigation effects on wood density at various heights for Pinus radiata

Wielinga, Benjamin; Waterworth, Rob; Brack, Cristopher
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
67.02%
This study examined the combined effect of 19-years of nitrogen fertilisation and irrigation on the basic density along the stems of Pinus radiata (D.Don). The sample trees came from two treatments in the Biology of Forest Growth experiment: the control (

Genetics of wood stiffness and its component traits in Pinus radiata

Kain, Dominic; Dungey, Heidi S; Matheson, A C; Evans, Robert
Fonte: NRC Research Press Publicador: NRC Research Press
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
66.88%
The potential for breeding Pinus radiata D. Don to improve wood stiffness (modulus of elasticity, MoE) was examined by obtaining pith-to-bark cores from trees at breast height in two independent genetic trials. The effectiveness of early selection for sti

A comparison between bark extracts from Pinus pinaster and Pinus radiata: Antioxidant activity and procyanidin composition

Jerez, María; Selga, Ariadna; Sineiro, Jorge; Torres, Josep Lluís; Núñez, María José
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 19968 bytes; application/msword
ENG
Relevância na Pesquisa
67.15%
5 pages, 4 tables.-- Printed version published Feb 2007.; The composition and antiradical activity of procyanidins from the bark of two kinds of pine, Pinus pinaster and Pinus radiata, were compared. Both the total bark extract and the fraction soluble in both water and ethyl acetate (OW) were evaluated, because of their promising results in previous experiments.; Results showed that P. radiata bark was richer in total phenols and also in procyanidins, catechin always being the main unit, so terminal as extensional. For P. pinaster, epicatechin was the predominant extension unit. The mean degree of polymerization (mDP) was higher for the latter. Interestingly, opposite results were encountered for the corresponding OW fractions, where P. radiata showed a mDP of 2.9 vs. 2.3 of P. pinaster. It was also found that the higher the mDP the higher was the specific antiradical activity. The different procyanidin composition and specific antiradical activity of the two kinds of barks, and particularly their OW fractions, may lead to the design of efficient natural antioxidants with application in the food industry.; We thank the Spanish Ministry of Science and Technology for Project No. PPQ2003-06602-C04, Subprojects 1 and 2.; Peer reviewed

Biomass and morphology of Pinus radiata coarse root components in a sub-humid temperate silvopastoral system

Gautam, A Madan; Mead, Donald; Clinton, Peter; Chang, Scott X
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
67.01%
Understanding the dynamics and distribution of root system components and how they are affected by pasture-tree interactions in silvopastoral systems are important for better management of agroforestry systems. The biomass and morphology of coarse root components were studied for 3- and 4-year-old Pinus radiata clonal and seedling trees growing with or without lucerne (Medicago sativa). Root:shoot ratio and lateral and vertical root biomass were greater by 1.5, 2.3 and 6.1 times, respectively, in clonal than in seedling trees, particularly in the no understory treatment compared to the lucerne treatment. Fractional allocation of root biomass to lateral and vertical roots was higher in clonal than in seedling trees by 1.1 and 2.6 times, respectively, while allocation to the root core was 1.4 times higher in the seedlings than the clone. Competition from lucerne reduced fractional allocation of root biomass to lateral roots by 40% in the seedling tree in 1993 and increased allocation to the root core. Competition was more intense at age 3 than at 4 years. No competition effect on lateral roots was observed with the clonal trees. Radiata pine root systems showed strong morphological plasticity to respond to changing soil conditions. Ripping coupled with thinning increased lateral root growth in the ripped zone so that by age 4 years 60% of lateral roots was in this zone. This was probably due to alterations in soil structure and reduced competition...

Density loss and respiration rates in coarse woody debris of Pinus radiata, Eucalyptus regnans and Eucalyptus maculata

Mackensen, Jens; Bauhus, Juergen
Fonte: Pergamon-Elsevier Ltd Publicador: Pergamon-Elsevier Ltd
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
67.14%
This study compared field and laboratory decomposition rates of coarse woody debris (CWD) (> 10 cm diameter) from three tree species: Pinus radiata, Eucalyptus regnans, and Eucalyptus maculata. For this purpose, the density loss of logs on the ground sampled from chronosequences of sites following harvesting was determined using the water replacement technique. P. radiata logs were sampled 1, 2.5, 6, and 9 years following harvesting, and logs of E. regnans and E. maculata were collected from sites that were harvested 1, 3.5, 6.5, and 12 and 1.5, 6.5, and 11.5 years ago, respectively. In addition, the C/N ratio of wood was determined and current respiration rates of logs from these different age classes were measured through laboratory incubation. The times for loss of 95% of material (t0.95) determined from density loss for these species were 24 years for P. radiata, 43 years for E. regnans, and 62 years for E. maculata. The decomposition rates of CWD derived from laboratory respiration were 6.1, 5.9 and 11.9 times higher than the decay rates from density loss in P. radiata, E. regnans, and E. maculata, respectively. This points to severe constraints of decomposition through adverse conditions in the field. The changes in respiration rates and C/N ratio with age of decaying logs indicated that the single component...

The effect of water status and soil fertility on the C-isotope signature in Pinus radiata

Korol, R L; Kirschbaum, Miko U. F.; Farquhar, Graham; Jeffreys, M
Fonte: Heron Publishing Publicador: Heron Publishing
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
66.88%
The efficiency with which trees use water is a major determinant of growth under water-limited conditions. We investigated whether increased access to water and nutrients alters water-use efficiency in Pinus radiata D. Don. Intrinsic transpiration efficiency, defined here as the ratio of CO2 assimilated and water transpired at a given vapor pressure deficit, is determined by the difference between ambient atmospheric CO2 concentration (C(a)) and leaf intercellular CO2 concentration (C(i)). The mean value of c(i)/c(a) can be inferred from an analysis of carbon isotope discrimination (Δ) in wood samples. A total of 117 trees, growing at sites with widely varying soil and climatic conditions in Australia and New Zealand, were cored and distinct annual rings were analyzed for their carbon isotope ratio, and correlated with rainfall during the July-June growing season in the year in which the wood was grown. Where possible, carbon isotope ratios were compared for different years within the same trees. The c(i)/c(a) ratio decreased with decreasing water availability, suggesting that intrinsic transpiration efficiency increased with decreasing water availability. An increase in growing season rainfall of 900 mm resulted in an increase in Δ of about 2.0‰...

Assessment and forecasting of mechanical properties for the 30-year-old Pinus radiata D. Don BY means of the multiple regression system.; ESTIMACIÓN Y PREDICCIÓN DE LAS PROPIEDADES MECÁNICAS En Pinus radiata D. Don DE 30 AÑOS DE EDAD MEDIANTE REGRESIÓN MÚLTIPLE

Rozas, Carlos; Vargas Mc, Gilda; Anzaldo H, José
Fonte: FUPEF DO PARANÁ Publicador: FUPEF DO PARANÁ
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Artigo Avaliado pelos Pares Formato: application/pdf
Publicado em 04/05/2007 POR
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This paper presents the results of FONDEF Project D9712006 developed at Bío-Bío University (Chile). The objetive of the study was to develop multiple regression models to estimate mechanical properties of Pinus radiata D. Don wood taking into account physical properpies, wood type (juvenile and adult wood) and different wood source (regions). The models developed allow to obtain good estimates of the mechanical propierties. Parallel hardness, perpendicular hardness, MOR, MOE and maximum stress in parallel compression presented the highest determining coefficients, being 0.82; 0.84; 0.80; 0.70 and 0.70, respectively.; Este estudio presenta los resultados del Proyecto FONDEF D97I2006 desarrollado en la Universidad del Bío-Bío (Chile). El objetivo del estudio fue desarrollar modelos de regresión múltiple para estimar las propiedades mecánicas de la madera de Pinus radiata D. Don, considerando las propiedades físicas de la madera, tipo de madera (juvenil y adulta) y diferentes zonas de crecimiento. Los modelos desarrollados permitieron obtener una buena estimativa de las propiedades mecánicas. La dureza paralela, dureza perpendicular, el MOR, el MOE y el esfuerzo máximo en compresión paralela...

Crecimiento en volumen y estado nutricional de Eucalyptus globulus Labill y Pinus radiata D. Don en Asturias, España

Khouri,Elias Afif; Canga Líbano,Elena; Oliveira Prendes,José Alberto; Gorgoso Varela,José Javier; Cámara Obregón,María Asunción
Fonte: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias Publicador: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2010 ES
Relevância na Pesquisa
67.01%
Se estudiaron las propiedades del suelo que más influyen en el crecimiento en volumen y el estado nutricional de árboles en plantaciones de Eucalyptus globulus y Pinus radiata en la parte centro-oriental de Asturias, España. Para cada especie se consideraron 10 clases diamétricas de cinco centímetros de amplitud y se derribaron tres árboles dominantes por clase y taxón de edades comprendidas entre 13 y 52 años. Se evaluaron en acículas recolectadas del tercio superior mediante el método de los niveles críticos. Las deficiencias más importantes fueron en P, K y Mg para P. radiata y en N, P y K para E. globulus. Los suelos se muestrearon a una profundidad de 0-20 cm y presentaron un carácter muy ácido, alto contenido de materia orgánica, relación C/N elevada, bajo contenido en bases y P disponible extraído por el método de Mehlich 3, en los dos taxa. El incremento anual medio en volumen con corteza (IAVC) estuvo asociado significativamente con el contenido de Ca en acículas de P. radiata, pero se obtuvo una correlación negativa para dicho elemento y el contenido de P asimilable en el suelo y positiva con el pH. En E. globulus, el IAVC se correlacionó positivamente con el K cambiable, Mg foliar y el P asimilable en el suelo...

Reactivación de material vegetal élite de pinus radiata d. Don. Mediante microinjerto in vitro

Materán,María Elena; Vega,María Carolina; Sánchez-Olate,Manuel; Sáez,Katia; Rodríguez,Roberto; Ríos,Darcy
Fonte: ASOCIACIÓN INTERCIENCIA Publicador: ASOCIACIÓN INTERCIENCIA
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2008 ES
Relevância na Pesquisa
66.96%
Se evaluaron dos protocolos de asepsia, tipos de púa (yemas y braquiblastos), épocas de recolección (otoño y verano) y posición de la púa (apical y basal) en el ortet, sobre el establecimiento y consolidación de microinjertos de Pinus radiata D. Don. Las púas se obtuvieron de clones adultos cultivados en vivero. Las asepsias consistieron en la inmersión en hipoclorito de sodio 2,5% v/v i.a, durante 15min (A1); o por 20min, seguido de la inmersión en una solución de benomyl+cisteína 50mg·l-1 c/u, hasta su utilización (A2). Como patrón se utilizaron hipocótilos provenientes de semillas germinadas in vitro en medio QL sin reguladores de crecimiento. Se realizaron microinjertos según la técnica apical de cuña. Estos se mantuvieron en tubos con medio QL + 0,1 mg·l-1 AIB y 1mg·l-1 BAP, a 25 ±2°C, intensidad lumínica de 80µmol·m-2·s-1 y fotoperíodo de 16h. Se utilizó un diseño completamente al azar en arreglo factorial (2(4)), con 13 repeticiones. El establecimiento mostró diferencias significativas entre las interacciones tipo de asepsia/época del año y tipo de púa/época del año, resultando ser las yemas apicales recolectadas durante el otoño y la asepsia tipo A2, los mejores tratamientos para el establecimiento de los microinjertos de P. radiata. La consolidación estuvo influenciada por el tipo de púa y la época del año en que se realizaron los microinjertos...