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Yeast biodiversity from oleic ecosystems: Study of their biotechnological properties

Romo-Sanchez, Sheila; Alves-Baffi, Milla; Arevalo-Villena, Maria; Ubeda-Iranzo, Juan; Briones-Perez, Ana
Fonte: Academic Press Ltd Elsevier B.V. Ltd Publicador: Academic Press Ltd Elsevier B.V. Ltd
Tipo: Artigo de Revista Científica Formato: 487-492
ENG
Relevância na Pesquisa
46.73%
The aim of this study was to know the yeast biodiversity from fresh olive (Olea europaea L.) fruits, olive paste (crush olives) and olive pomace (solid waste) from Arbequina and Cornicabra varieties. Yeasts were isolated from fruits randomly harvested at various olive groves in the region of Castilla La Mancha (Spain). Olive paste and pomace, a byproduct of the processing of this raw material, were also collected in sterile flasks from different oil mills. Molecular identification methodology used included comparison of polymerase chain reaction (PCR) amplicons of their 5.8S rRNA gene and internal transcribed spacers ITS1 and ITS2 followed by restriction pattern analysis (RFLP). For some species, sequence analysis of the 5.8S rDNA gene was necessary. The results were compared to sequences held in public databases (BLAST). These techniques allowed to identify fourteen different species of yeasts, belonging to seven different genera (Zygosaccharomyces, Pichia, Lachancea, Kluyveromyces, Saccharomyces, Candida, Torulaspora) from the 108 yeast isolates. Species diversity was thus considerable: Pichia caribbica, Zygosaccharomyces fermentati (Lachancea fermentati) and Pichia holstii (Nakazawaea holstii) were the most commonly isolated species...

Quantificação de estigmatadienos em azeite de oliva (Olea europea L.) por cromatografia em fase gasosa.; Stigmastadiene quantification in olive oil (Olea europaea L.) by gas chromatography.

Juliana Stefanon Modolo
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 25/07/2007 PT
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56.79%
O azeite de oliva é um produto com alto valor nutricional e tem conquistado seu espaço no mercado consumidor devido às suas marcantes características organolépticas. O cultivo e a colheita de olivas e a extração do azeite são atividades trabalhosas e, por isso, o azeite de oliva tem alto custo comercial. Por conseqüência, as tentativas de adulteração deste produto com outros tipos de azeites mais baratos, como o azeite de oliva refinado, por exemplo, é comum. Este trabalho visa despertar um maior interesse dos órgãos de vigilância e de controle de qualidade de azeites de oliva, mostrando a importância da aplicação de metodologias específicas para evidenciar adulterações deste produto. Este estudo propôs a quantificação de estigmastadienos como a principal técnica capaz de identificar fraudes em azeites de oliva extra virgem e virgem. Este método analítico consiste na saponificação da amostra de azeite na presença de padrão interno (colesta-3,5-dieno), extração e fracionamento dos componentes insaponificáveis por cromatografia em coluna aberta, seguida pela identificação e quantificação do estigmasta-3,5-dieno e seu isômero por cromatografia em fase gasosa com detector de ionização de chama. A separação cromatográfica do estigmastadieno foi realizada em coluna capilar de sílica fundida HP-5 (5% difenil 95% dimetil polisiloxano...

Valorisation of olive pomace by solid-state fermentation with Aspergillus species for lipase production

Oliveira, Felisbela Maria Araújo; Moreira, Cláudia; Salgado, José Manuel; Abrunhosa, Luís; Venâncio, Armando; Belo, Isabel
Fonte: WASTES 2013; Centro para a Valorização de Resíduos (CVR) Publicador: WASTES 2013; Centro para a Valorização de Resíduos (CVR)
Tipo: Conferência ou Objeto de Conferência
Publicado em //2013 ENG
Relevância na Pesquisa
66.83%
Pollution by olive mill wastes is a crucial problem in Mediterranean area and their proper management and utilization is demanded. Olive pomace offers excellent properties to produce enzymes by solid-state fermentation (SSF) using filamentous fungi. Particularly for lipase production, since it has residual content of olive oil. The aim of this work was to optimize the production of lipase by Aspergillus ibericus MUM 03.49, Aspergillus niger MUM 03.58 and Aspergillus tubingensis MUM 06.152, under SSF of olive pomace. A Taguchi L-9 orthogonal array based on 4 factors at 3 levels (ratio between olive pomace and wheat bran (OP:WB), NaNO3, Czapek nutrients and time) was implemented. SSF was carried out in Erlenmeyer flasks of 500 mL and lipase activity was measured using p-nitrophenyl butyrate as substrate. Results showed for all fungi that the factor with most significant effect on lipase production was the mixture OP:WB, concluding that the presence of wheat bran on substrate favored lipase production. NaNO3 concentration and time presented some effect and presence of Czapek nutrients did not added significant advantages on lipase production. A. ibericus was the best lipase producer, being a promising microorganism for lipase production. Under optimized conditions it produced 20.78 U/gds of lipase.

Management of agro-industrial wastes: utilization of exhausted grape mark as co-substrate with olive pomace for cellulases production by SSF

Salgado, José Manuel; Oliveira, Felisbela Maria Araújo; Abrunhosa, Luís; Venâncio, Armando; Belo, Isabel
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em /09/2013 ENG
Relevância na Pesquisa
46.83%
The reutilization of biological wastes is of great interest since, due to legislation and environmental reasons, the industry is increasingly being forced to find an alternative use for its residues. Moreover, the use of these wastes considerably reduces the production costs. Olive oil and wine production are the most common food processing activities in the countries of southern Europe. Environmental pollution posed by olive mill wastes is a growing problem especially in the Mediterranean region. Research into finding new uses for wastes from olive oil and wine industry will allow obtaining not only economic benefits, but also environment improvements in areas where industries are located. In previous studies, several wastes from wineries and olive mills were tested for cellulase production by solid state fermentation (SSF). It was observed that mixture of exhausted grape mark (EGM) with olive pomace (OP) were suitable substrates for cellulase production. After fungi screening study, Aspergillus ibericus showed more cellulase activity. To optimize cellulase production a full factorial experimental design 32 was planned. The independent variables were % of EGM mixture with olive pomace and urea supplementation and response variable was cellulase activity (U/gds). The obtained results showed that these agro-industrial wastes were suitable for the production of cellulases...

Influence of moisture content, temperature and inoculum size on lipase production by filamentous fungi under solid-state fermentation of olive pomace

Oliveira, Felisbela Maria Araújo; Salgado, José Manuel; Abrunhosa, Luís; Venâncio, Armando; Belo, Isabel
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em 04/06/2013 ENG
Relevância na Pesquisa
46.69%
Pollution by olive mill wastes is a crucial problem in the Mediterranean area and proper management is necessary. Olive pomace, a solid by-product generated by the olive oil two phase extraction process, is an acidic and very humid material, rich in organic matter, potassium, nitrogen, carbohydrates, phenols and also contains residual fats, which make an interesting substrate for lipase production under solid-state fermentation. The aim of this work was to optimise moisture content (MC), temperature and inoculum size, in order to improve lipase production by Aspergillus ibericus MUM 03.49, Aspergillus niger MUM 03.58 and Aspergillus tubingensis MUM 06.152, under solid-state fermentation (SSF) of olive pomace mixed with wheat bran. Firstly, a full factorial design of experiments was implemented in order to investigate the effect of MC (70%, 75% and 80% wet basis) and temperature (25 ºC, 30 ºC and 35 ºC) on its production. SSF was carried out in 500 mL Erlenmeyer flasks, containing 30 g solid dry substrate. Lipase activity was measured by colorimetric assay, using p-nitrophenyl butyrate as substrate. In general, MC presented a significant effect (p < 0.05) on lipase production. Temperature presented a significant effect only for A. ibericus. Maximum lipase production was obtained at 70% of MC for all microorganisms...

Optimization of initial moisture and temperature in solid-state fermentation to produce cellulases by Aspergillus uvarum on olive mill and winery wastes

Salgado, José Manuel; Abrunhosa, Luís; Domínguez, José Manuel; Venâncio, Armando; Belo, Isabel
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em //2014 ENG
Relevância na Pesquisa
46.37%
Wineries and olive oil industries are dominant agro-industrial activities in southern European regions. The use of byproducts of these industries could reduce the costs of enzymes production. Olive pomace, exhausted grape marc, vineshoot trimmings are lignocellulosic materials, thus they have potential to be used as substrate for cellulase production by solid-state fermentation. Since, moisture and temperature are important parameters on SSF, a full factorial design was planned to optimize these parameters. Optimum conditions were 29 ºC and 75% of moisture, and 33.49 U/g of cellulose activity were achieved.

Contribuição do sector do azeite para a produção de biodiesel

Sebadelhe, Cláudia Sofia Moutinho
Fonte: Universidade Técnica de Lisboa Publicador: Universidade Técnica de Lisboa
Tipo: Dissertação de Mestrado
Publicado em //2008 POR
Relevância na Pesquisa
67.02%
Mestrado em Olivicultura, Azeite e Azeitona de mesa - Instituto Superior de Agronomia; The olive oil sector, with a strong tradition in Portugal, can be a source of raw materials for biodiesel production. The olives infected by plages and diseases, will generate lampante virgin olive oil without food quality and the crude olive pomace oil extracted from the pomace is not appropriate for food consumption. The purpose of the present investigation was to study the biodiesel production from lampante virgin olive oil and crude olive pomace oil with acid values much higher than 1 %. In this study, two different processes for biodiesel production were tested and compared with these raw materials. One, by using the traditional alkaline catalyzed transesterification process, the other by a two step process using acid catalyzed esterification followed by alkaline catalyzed transesterification. The acid catalyzed esterification process was optimized by application the response surface methodology. The highest yields of fatty acid methyl esters were obtained by the two step process. The properties of the biodiesel obtained from both raw materials were determined and most of the parameters were in accordance to the specifications of biodiesel standard. Only the oxidative stability did not reach the minimum value established in these specifications.

Extracção e encapsulamento de compostos bioactivos do bagaço de azeitona

Duarte, Cláudia Sofia Caeiro
Fonte: ISA/UTL Publicador: ISA/UTL
Tipo: Dissertação de Mestrado
Publicado em //2011 POR
Relevância na Pesquisa
36.66%
Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia; The food industry by-products such as olive oil processing solid waste are an important source of natural bioactive compounds. The aim of the present work was to extract bioactive compounds from olive pomace using supercritical carbon dioxide extraction and to investigate the viability of their encapsulation in a food-stuff carrier as a way of protection from antioxidant properties losses. Several supercritical extractions were performed at a 250 bar and a 40 ºC with and without pre-treatments and levels of moisture content were also tested. Yields between 8 and 12% (w/w) were achieved and fatty acid content, sterols and total poliphenolic compounds were evaluated in all supercritical extracts. Since there are a strong correlation between antioxidant activity and poliphenolic compounds, the two most representative phenols, hydroxytyrosol and tyrosol, were identified and quantified. Antioxidant activity was also investigated using ORAC and HORAC methods. Extraction of bioactive compounds from olive pomace in soxhlet apparatus were also carried out for control and yields of 13% (w/w) were achieved. Encapsulation of the most bioactive extract achieved was performed by spray-drying using inulin as the wall material and antioxidant activity was evaluated.

Otimização de meios de cultura para a produção de xilitol com base em hidrolisados hemicelulósicos de bagaço de azeitona extratado

Morais, Ana Rita Colaço
Fonte: ISA/UTL Publicador: ISA/UTL
Tipo: Dissertação de Mestrado
Publicado em //2012 POR
Relevância na Pesquisa
56.62%
Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia; The extracted olive pomace (EOP) is the by-product of the olive pomace oil extraction industry, currently used in low value energy applications. In this work, it was studied the valorisation of hemicellulosic fraction of this material to development of the production of pentose-rich hydrolysates for subsequent bioconversion into xylitol. Given the high content of phenolic compounds, some high value-added, it was studied the aqueous pre-extraction (APE). The optimum conditions for APE were found at 100 ºC, 60 min and a liquid to solid ratio (LSR) of 5 g·g-1. To obtain hemicellulosic hydrolysates, it was studied for both APE-treated and untreated EOP using dilute sulphuric acid (3.5%, w/w) at 130ºC for 130 min at different LSR (3, 4 and 5). The hydrolysates obtained were detoxified using different methods which include the adsorption to NaOH and HCl activated charcoal, anionic resins, concentration by evaporation and membranes (nanofiltration). The impact of hydrolysis LSR, detoxification method and supplementation on xylitol production by the yeast D. hansenii, was evaluated. Nanofiltration was the method that enabled the highest removal of inhibitors. The highest xylitol volumetric productivity was 0.30 g·L-1·h-1 (yield 0.42 g·g-1) was obtained for resin-detoxified hydrolysates (pH 5.5). The highest yield...

Agro-food industry residues for biodiesel production: BIOFFA project

Costa, Paula; Bogel-Lukasik, R.; Passarinho, Paula C.; Oliveira, A. C.
Fonte: Laboratório Nacional de Energia e Geologia Publicador: Laboratório Nacional de Energia e Geologia
Tipo: Conferência ou Objeto de Conferência
Publicado em //2013 ENG
Relevância na Pesquisa
46.33%
The aim of the project BIOFFA is to develop processes for the production of biofuels from residual raw-materials with high free fatty acid (FFA). In technological terms, two distinct approaches, leading to different final products, are being assessed: production of fatty acid methyl esters (FAME) – biodiesel, and hydrogenated oil – H-oil. Different residues available in Portugal, including poultry fat, cattle fat, olive pomace oil and used frying oils, were collected and characterised, and the objectives of the project will be considered to be met if it will be possible to produce mixtures of both biofuels (biodiesel + H-oil) similar to the nowadays commercially available formulas (biodiesel + petro-diesel) with the superior advantages of valorising residues and producing the overall mixture from biological materials.

Production of bio-hydrocarbons by hydrotreating of pomace oil

Pinto, Filomena; Varela, F. T.; Gonçalves, Margarida; André, Rui Neto; Costa, Paula; Mendes, Benilde
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em //2014 ENG
Relevância na Pesquisa
77.15%
Olive pomace oil is a by-product from the olive oil industry that is still being used in the food industry as a low value vegetable oil. Crude olive pomace oil needs to be refined and is blended with virgin olive oils before being used as edible oil. The detection of toxic compounds led to more restricted legislation and to the search of alternative valorisation processes, such as hydrotreating to obtain bio-hydrocarbons. Hydrotreating of olive pomace oil at moderate temperatures (from 300 to 430 C) and in presence of initial hydrogen pressure of 1.1 MPa led to triglycerides destruction and to their conversion into a large range of organic compounds with predominance to hydrocarbons. Even without any catalyst, conversions into hydrocarbons were always higher than 90% (v/v). Catalyst presence, such as: CoMo/Al2O3, FCC (fluid catalytic cracking) or HZSM-5 changed hydrogenated liquids composition. The highest content of alkanes was obtained with CoMo catalyst, while FCC and HZSM-5 led to the highest contents of aromatic compounds. The results obtained showed that olive pomace oil can be efficiently converted into bio-hydrocarbons with a wide range of applications. It was also studied the effect of pyrolysing olive pomace oil prior to its hydrotreating. Pyrolysis pre-treatment seems to have favoured hydrotreating process by promoting initial cracking reactions. Thus...

Stigmastadiene and specific extitntion (270 nm) to evaluate the presence of refined oils in virgin olive oil commercialized in Brazil

Aued-Pimentel,Sabria; Silva,Simone Alves Da; Takemoto,Emy; Cano,Cristiane Bonaldi
Fonte: Sociedade Brasileira de Ciência e Tecnologia de Alimentos Publicador: Sociedade Brasileira de Ciência e Tecnologia de Alimentos
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2013 EN
Relevância na Pesquisa
46.71%
The increased marketing of olive oil in Brazil has intensified legal requirements to ensure regulation of this product. The measurement of the specific extinction at 270 nm (E 270) and content of stigmastadiene can be used to assess the presence of refined oils in virgin olive oil. During the vegetable oil refining process, compounds with conjugated double bonds are generated from unsaturated fatty acids that absorb at 270 nm and sterols, such as stigmasta-3,5-diene. To compare these parameters, seven samples of extra virgin olive oil and three samples of olive oil (blend of virgin and refined) were analyzed. Among the samples analyzed, four extra virgin samples had levels of stigmastadiene and E 270 higher than expected, among which two were adulterated with seed oil (rich in linoleic acid) and the other two with olive pomace oil. The results demonstrate the higher sensitivity of stigmastadiene to determine the presence of the refined oil in virgin olive oil and good agreement with determining E 270. The latter technique is a simple, quick, and low cost method of determination that can be easily implemented in laboratories to assist in the screening and regulation of olive oils sold in Brazil.

Antithrombotic and Antiatherosclerotic Properties of Olive Oil and Olive Pomace Polar Extracts in Rabbits

Tsantila, Nektaria; Karantonis, Haralabos C.; Perrea, Despina N.; Theocharis, Stamatios E.; Iliopoulos, Dimitrios G.; Antonopoulou, Smaragdi; Demopoulos, Constantinos A.
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.66%
Olive oil polar lipid (OOPL) extract has been reported to inhibit atherosclerosis development on rabbits. Olive pomace polar lipid (PPL) extract inhibits PAF activity in vitro and the most potent antagonist has been identified as a glycerylether-sn-2-acetyl glycolipid with common structural characteristics with the respective potent antagonist of OOPL. The aim of this study was to investigate the effect of PPL on early atherosclerosis development on rabbits and to compare it with the antiatherosclerotic effect of OOPL. OOPL and PPL inhibition potency, towards both PAF action and PAF binding, was tested in vitro on washed rabbit platelets. Consequently, rabbits were divided into three groups (A, B, and C). All groups were fed atherogenic diet for 22 days. Atherogenic diets in groups B and C were enriched with OOPL and PPL, respectively. At the end of the experimental time, rabbits were euthanized and aortic samples were examined histopathologically. OOPL and PPL inhibited PAF-induced aggregation, as well as specific PAF binding, with PPL being more potent. Free and bound PAF levels and PAF-AH activity were significantly elevated at the end of the experimental time. Plasma total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides levels were also found increased. Groups B and C exhibited significantly increased values of EC50 compared to group A. Histopathological examination revealed that the development of early atherosclerosis lesions in groups B and C were significantly inhibited compared to group A. Significant differences were noted in the early atherosclerosis lesions between groups B and C...

Thin Layer Drying Kinetics of By-Products from Olive Oil Processing

Montero, Irene; Miranda, Teresa; Arranz, Jose Ignacio; Rojas, Carmen Victoria
Fonte: Molecular Diversity Preservation International (MDPI) Publicador: Molecular Diversity Preservation International (MDPI)
Tipo: Artigo de Revista Científica
Publicado em 15/11/2011 EN
Relevância na Pesquisa
36.77%
The thin-layer behavior of by-products from olive oil production was determined in a solar dryer in passive and active operation modes for a temperature range of 20–50 °C. The increase in the air temperature reduced the drying time of olive pomace, sludge and olive mill wastewater. Moisture ratio was analyzed to obtain effective diffusivity values, varying in the oil mill by-products from 9.136 × 10−11 to 1.406 × 10−9 m2/s in forced convection (ma = 0.22 kg/s), and from 9.296 × 10−11 to 6.277 × 10−10 m2/s in natural convection (ma = 0.042 kg/s). Diffusivity values at each temperature were obtained using the Fick’s diffusion model and, regardless of the convection, they increased with the air temperature. The temperature dependence on the effective diffusivity was determined by an Arrhenius type relationship. The activation energies were found to be 38.64 kJ/mol, 30.44 kJ/mol and 47.64 kJ/mol for the olive pomace, the sludge and the olive mill wastewater in active mode, respectively, and 91.35 kJ/mol, 14.04 kJ/mol and 77.15 kJ/mol in natural mode, in that order.

Enhancing the bioconversion of winery and olive mill waste mixtures into lignocellulolytic enzymes and animal feed by Aspergillus uvarum using a packed-bed bioreactor

Salgado, José Manuel; Abrunhosa, Luís; Venâncio, Armando; Domínguez, José Manuel; Belo, Isabel
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artigo de Revista Científica
Publicado em 13/07/2015 ENG
Relevância na Pesquisa
46.37%
Wineries and olive oil industries are dominant agro-industrial activities in southern European regions. Olive pomace, exhausted grape marc and vine shoot trimmings are lignocellulosic residues generated by these industries, which could be valued biotechnologically. In the present work these residues were used as substrate to produce cellulases and xylanases through solid-state fermentation using Aspergillus uvarum. For that, two factorial designs (32) were first planned to optimize substrate composition, temperature and initial moisture level. Subsequently, the kinectics of cellulolytic enzymes production, fungal growth and fermented solid were characterized, Finally, the process was performed in a packed-bed bioreactor. The results showed that cellulase activity improved with the optimization processes, reaching 33.56 U/g, and with the packed-bed bioreactor aeration of 0.2 L/min, reaching 38.51 U/g. The composition of fermented solids indicated their potential use for animal feed because cellulose, hemicellulose, lignin and phenolic compounds were partially degraded 28.08, 10.78, 13.3 and 28.32% respectively, crude protein was increased from 8.47% to 17.08%, and, the mineral contents meet the requirements of main livestock.

Sonication of olive pomace to improve xylanases production by SSF

Leite, P.; Salgado, José Manuel; Abrunhosa, Luís; Venâncio, Armando; Belo, Isabel
Fonte: Taylor & Francis Publicador: Taylor & Francis
Tipo: Conferência ou Objeto de Conferência
Publicado em /09/2015 ENG
Relevância na Pesquisa
46.69%
Olive mill wastes are an important environmental problem in the areas where they are generated. Portugal is an important producer of olive oil in the world, thus it is interesting to find the most suitable valorization strategy to exploit their wastes which are generated in huge quantities in short periods of time. In this study, it was used the olive pomace as solid substrate to produce xylanases and cellulases by filamentous fungi. To improve the enzymes production ultrasounds pretreatment of olive pomace was evaluated. The results showed that the sonication led to a 3-fold increase of xylanase activity and a decrease of cellulase activity, indicating that ultrasounds treatment attacked the integrity of cell walls and increased the accessibility of hemicelluloses inducing the xylanases production by fungi.

Orujos de dos fases almacenados en balsas; Two phases olive pomace storaged in ponds

Ramos Hinojosa, Álvaro E.; Ruiz Méndez, Mª Victoria
Fonte: Consejo Superior de Investigaciones Científicas (España) Publicador: Consejo Superior de Investigaciones Científicas (España)
Tipo: Artículo
SPA
Relevância na Pesquisa
67.01%
During the harvesting period 1999-2000, real samples of two phases olive pomace were taken from storage ponds in two extraction facilities located in Southern region of Spain, every month until a maximum of five months. In addition, lots of two phases olive pomace and second-centrifuged two phases olive pomace were divided in closed recipients and, also, in opened recipients, and were stored in laboratory at 36ºC for 0, 1, 2, 4 and 6 months. Samples were analysed for moisture and fat content, and solvent-extracted; the resulting oils were analysed for acidity, fatty acid composition, polar compounds and triglycerides. The results indicate that oil deterioration increases throughout the storage period, particularly those oils extracted from surface and from second-centrifuged two phases olive pomace. The surface/volume ratio in the ponds should be as low as possible. Special attention should be paid to the storage of two phases olive pomace, establishing a maximum of two months in all cases.; Durante la campaña 1999-2000 se han tomado muestras reales de orujo de balsas de almacenamiento, cada mes hasta un máximo de 5 meses, y de lotes de orujo de dos fases almacenados en el laboratorio, en recipientes cerrados y abiertos, a 36 ºC durante 0...

Production of pomace olive oil; Obtención de aceite de orujo

Sánchez Moral, Pedro; Ruiz Méndez, Mª Victoria
Fonte: Consejo Superior de Investigaciones Científicas (España) Publicador: Consejo Superior de Investigaciones Científicas (España)
Tipo: Artículo
ENG
Relevância na Pesquisa
46.94%
Pomace oil is the principal by-product in olive oil processing and is currently developing considerably due to the technological advances which the mills have undergone in recent years. This investigation aims at presenting an overview of the complexity of this sector which, with around 100 years of existence, is searching for a role in the new integral strategy of the olive oil industry after the crisis suffered in 2001. Physical extraction, drying and solvent extraction are discussed, along with the influence of the technological treatments on the chemical composition of pomace oil.; El aceite de orujo es el principal subproducto en el procesado de aceite de oliva y su obtención se ha transformado considerablemente en los últimos tiempos debido a los avances tecnológicos que han experimentado las almazaras. Este trabajo tiene la finalidad de presentar una panorámica de la complejidad de este sector, de casi 100 años de existencia, que se afana en encontrar su función dentro de la nueva estrategia integral de la industria oleícola después de la crisis sufrida en el año 2001. En el mismo se comentan la extracción física, el secado, así como la extracción con disolvente, y la influencia que tienen los tratamientos tecnológicos sobre la composición química del aceite de orujo.; Peer reviewed

Treatment technologies of liquid and solid wastes from two-phase olive oil mills; Tecnologías de tratamiento de los efluentes líquidos y residuos sólidos resultantes del proceso de elaboración del aceite de oliva por centrifugación en dos fases

Borja Padilla, Rafael; Raposo Bejines, Francisco; Rincón, Bárbara
Fonte: Consejo Superior de Investigaciones Científicas (España) Publicador: Consejo Superior de Investigaciones Científicas (España)
Tipo: Artículo
ENG
Relevância na Pesquisa
56.93%
Over the last 10 years the manufacture of olive oil has undergone important evolutionary changes in the equipment used for the separation of olive oil from the remaining components. The latest development has been the introduction of a two-phase centrifugation process in which a horizontally-mounted centrifuge is used for a primary separation of the olive oil fraction from the vegetable solid material and vegetation water. Therefore, the new two-phase olive oil mills produce three identifiable and separate waste streams. These are: 1) the wash waters from the initial cleansing of the fruit; 2) the wash waters from the secondary centrifuge and 3) the aqueous solid residues from the primary centrifugation. As well as offering process advantages they also reduce the water consumption of the mill. The introduction of this technology was carried out in 90% of Spanish olive oil factories. Therefore, the new twophase olive mill effluents (TPOME) are made up of the mixture of effluents (1) and (2), the total volume of TPOME generated being around 0.25 l/kg of olives processed. In addition, the solid residue (two-phase olive pomace, TPOP) has a high organic matter concentration giving an elevated polluting load and it cannot be easily handled by traditional technology which deals with the conventional three-phase olive cake. So...

Valorization of olive pomace through combination of biocatalysis with supercritical fluid technology

Nogueira, João Humberto Gonçalves Francisco
Fonte: Universidade Nova de Lisboa Publicador: Universidade Nova de Lisboa
Tipo: Dissertação de Mestrado
Publicado em /09/2015 ENG
Relevância na Pesquisa
77.15%
A supercritical carbon dioxide (scCO2) based oil extraction method was implemented on olive pomace (alperujo), and an oil yield of 25,5 +/- 0,8% (goil/gdry residue) was obtained. By Soxhlet extraction with hexane, an oil extraction yield of 28,9 +/- 0,8 % was obtained, which corresponds to an efficiency of 88,4 +/- 4,8 % for the supercritical method. The scCO2 extraction process was optimized for operating conditions of 50 MPa and 348,15 K, for which an oil loading of 32,60 g oil/kg CO2 was calculated. As a proof of concept, olive pomace was used as feedstock for biodiesel production, in a process combining the use of lipase as a catalyst with the use of scCO2 as a solvent, and integrating the steps of oil extraction, oil to biodiesel transesterification and subsequent separation of the latter. In the conducted experiments, FAME (fatty acid methyl ester) purities of 90% were obtained, with the following operating parameters: an oil:methanol molar ratio of 1:24; a residence time of 7,33 and 11,6 mins; a pressure of 40 MPa; a temperature of 313,15 K; and Lipozyme (Mucor miehei; Sigma-Aldritch) as an enzyme. However, oscillations of FAME purity were registered throughout the experiments, which could possibly be due to methanol accumulation in the enzymatic reactor. Finally...