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Os sufixos agentivos -nte e-(d/t/s)or no português: um estudo semântico-diacrônico; The agentive suffixes nte and (d/t/s)or in the Portuguese language: a semantic diachronic study

Oliveira, Anielle Aparecida Gomes Gonçalves Jacometti de
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 08/09/2014 PT
Relevância na Pesquisa
28.06%
No processo derivacional, os sufixos nte e (d/t/s)or são conhecidos por formarem vocábulos designados na língua como agentivos. O conceito de agente, por sua vez, é fundamental para os estudos da linguagem, pois descreve uma categoria primária a qualquer ser vivo, servindo, por isso, de base para a criação de outras categorias. Propôs-se observar, descrever e organizar os significados dos sufixos nte e (d/t/s)or presentes na língua portuguesa, utilizando o mecanismo da paráfrase. Por tratar-se de afixos abrangentes, fizeram-se necessários dois tipos de abordagem: uma qualitativa e uma quantitativa. Num primeiro momento, fez-se uma pesquisa etimológica específica nos agentivos nomeadores de profissionais, isto é, em designadores de seres humanos portadores de uma função, em que se examinou o emprego primitivo dos sufixos que compõem essa análise. Depois, abarcaram-se palavras pertencentes a campos diversos, limitados pela presença desses sufixos. Os sufixos formadores de nomina agentis nte e (d/t/s)or remetem aos falantes a ideia de alguém ou algo que faz alguma coisa, isto é, forma duas séries nas quais se corporizam as derivações deverbais de designações de pessoas que são agentes da ação implicada no significado do verbo. A hipótese do trabalho é a de que os nomes em nte e (d/t/s)or possuem a propriedade do aspecto...

Mechanisms for consideration for intervention in the development of organophosphorus-induced delayed neuropathy

Emerick, Guilherme Luz; DeOliveira, Georgino H.; dos Santos, Antonio C.; Ehrich, Marion
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 177-184
ENG
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 09/51048-8; Processo FAPESP: 12/00168-6; Organophosphorus-induced delayed neuropathy (OPIDN) is a neurodegenerative disorder characterised by ataxia progressing to paralysis with concomitant central and peripheral distal axonopathy. Symptoms of OPIDN in people include tingling of the hands and feet. This tingling is followed by sensory loss, progressive muscle weakness and flaccidity of the distal skeletal muscles of the lower and upper extremities and ataxia, which appear about 8-14 days after exposure. Some organophosphorus compounds (OPs) that are still used in worldwide agriculture have potential to induce OPIDN, including methamidophos, trichlorfon, dichlorvos and chorpyrifos. This review summarizes experimental attempts to prevent and/or treat OPIDN and the different mechanisms involved in each approach. The initial mechanism associated with development of OPIDN is phosphorylation and inhibition of neuropathy target esterase (NTE). The phosphorylated enzyme undergoes a second reaction known as aging that results in the loss of one of the R groups bound to the phosphorus of the OP. A second mechanism involved in OPIDN is an imbalance in calcium homeostasis. This can lead to the activation of calcium-activated neutral protease and increases in calcium/calmodulin-dependent protein kinases. These events contribute to aberrant phosphorylation of cytoskeletal proteins and protein digestion in the terminal axon that can proceed similarly to Wallerian-type degeneration. Several experimental studies demonstrated alleviation of the signs and symptoms of OPIDN by restoring calcium balance. Other studies have used preadministration of NTE inhibitors...

Avaliação de neurotoxicidade de formas enantioméricas de praguicidas organifosforados: estudos in vitro, in vivo e de neuroproteção

Emerick, Guilherme Luz
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Tese de Doutorado Formato: 174 f. : il., gráfs., tabs.
POR
Relevância na Pesquisa
27.56%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Pós-graduação em Ciências Farmacêuticas - FCFAR; Clinicamente, os sintomas da intoxicação por organofosforados (OPs) são divididos em quatro categorias: síndrome colinérgica, síndrome intermediária, transtornos neuropsiquiátricos crônicos induzidos por OPs e neuropatia retardada induzida por OPs (NRIOP). Há estudos que comprovam a ocorrência de casos de NRIOP em seres humanos após intoxicação por metamidofós, porém existe grande dificuldade de estudar esta neuropatia no modelo experimental (galinha), devido à forte crise colinérgica que ocorre após a inibição da acetilcolinesterase (AChE). Uma possível explicação para tal diferença de efeito entre a espécie humana e a galinha é o fato de que os OPs possuem centros assimétricos e, assim sendo, se apresentam sob formas enantioméricas que podem apresentar diferenças de inibição da AChE e da esterase susceptível à neuropatia (ESNp). Assim, o objetivo deste trabalho foi avaliar, em sangue de galinhas e de seres humanos, em cérebro de galinhas (in vitro e in vivo) e em células da linhagem SH-SY5Y de neuroblastoma humano, a neurotoxicidade de formas enantioméricas de compostos organofosforados utilizados como praguicidas...

Antropometria nutricional e ecologia humana dos Xav??nte de Sangradouro-Volta Grande, Mato Grosso

Gugelmin, Silvia Angela
Fonte: Fundação Oswaldo Cruz Publicador: Fundação Oswaldo Cruz
Tipo: Tese de Doutorado
PT_BR
Relevância na Pesquisa
27.89%
Tem como objetivo discutir o perfil ecol??gico-humano e de nutri????o dos Xav??nte, apresentando como eixo a an??lise comparativa de um conjunto de estudos realizados entre os Xav??nte de Pimentel Barbosa e de Sangradouro-Volta Grande. Os Xav??nte s??o um povo ind??gena cujas terras est??o localizadas em Mato Grosso e cujo contato permanente com a sociedade nacional aconteceu a partir da d??cada de 1940. A an??lise comparativa foi empregada a fim de investigar as diferen??as nas v??rias dimens??es da vida destes grupos, em especial ??quelas relacionadas as atividades de subsist??ncia, padr??es alimentares e perfis antropom??tricos, relacionando-as as diversas trajet??rias hist??ricas de contato com a sociedade nacional e inser????o no mercado regional. Durante o trabalho de campo foram coletados dados de identifica????o, aloca????o de tempo, antropometria, condi????es sanit??rias e econ??micas e crescimento f??sico das crian??as. Al??m disso, foram realizadas observa????es de campo sobre aspectos alimentares. A partir dos cinco artigos descritos nesta tese observou-se que a baixa estatura para idade ?? um problema relevante entre as crian??as Xav??nte menores de 5 anos; enquanto o sobrepeso e a obesidade afeta a grande maioria da popula????o adulta...

Avalia????o do estado nutricional da popula????o Xav??nte de S??o Jos??, Terra Ind??gena Sangradouro - Volta Grande, Mato Grosso

Leite, Maur??cio Soares
Fonte: Fundação Oswaldo Cruz Publicador: Fundação Oswaldo Cruz
Tipo: Dissertação
PT_BR
Relevância na Pesquisa
27.56%
Descreve o crescimento f??sico e a epidemiologia da anemia na popula????o Xav??nte da aldeia de S??o Jos??, Terra Ind??gena Sangradouro - Volta Grande, Mato Grosso. O inqu??rito antropom??trico inclui medidas de massa corporal, estatura (ou comprimento), circunfer??ncia braquial e dobra cut??nea tricipital de 546 indiv??duos de 0 a 90 anos. A preval??ncia e a distribui????o da anemia foram determinadas a partir da dosagem de hemoglobina, realizada em 238 indiv??duos desta mesma popula????o. A avalia????o do estudo nutricional de crian??as e adultos revela que entre os adultos j?? s??o comuns casos de obesidade, enquanto entre as crian??as a desnutri????o prot??ico-energ??tica ?? um problema freq??ente. Quando comparadas ?? popula????o-refer??ncia do NCHS, as crian??as Xav??nte s??o pequenas em termos de estatura para a idade, mas apresentam uma propor????o adequada entre massa corporal e estatura. De acordo com os crit??rios da Organiza????o Mundial de Sa??de (WHO, 1995), 31,7 por cento dos menores de 5 anos apresentam baixa estatura para a idade e seriam, portanto, diagnosticados como desnutridos. A compara????o com a popula????o brasileira revela m??dias de estatura bastante pr??ximas e mesmo superiores ??s brasileiras, particularmente a partir dos 5 anos de idade. Diante das prec??rias condi????es sanit??rias e da elevada preval??ncia de doen??as infecto-parasit??rias observadas na aldeia...

Evidence that mouse brain neuropathy target esterase is a lysophospholipase

Quistad, Gary B.; Barlow, Carrolee; Winrow, Christopher J.; Sparks, Susan E.; Casida, John E.
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
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Neuropathy target esterase (NTE) is inhibited by several organophosphorus (OP) pesticides, chemical warfare agents, lubricants, and plasticizers, leading to OP-induced delayed neuropathy in people (>30,000 cases of human paralysis) and hens (the best animal model for this demyelinating disease). The active site region of NTE as a recombinant protein preferentially hydrolyzes lysolecithin, suggesting that this enzyme may be a type of lysophospholipase (LysoPLA) with lysolecithin as its physiological substrate. This hypothesis is tested here in mouse brain by replacing the phenyl valerate substrate of the standard NTE assay with lysolecithin for an “NTE-LysoPLA” assay with four important findings. First, NTE-LysoPLA activity, as the NTE activity, is 41–45% lower in Nte-haploinsufficient transgenic mice than in their wild-type littermates. Second, the potency of six delayed neurotoxicants or toxicants as in vitro inhibitors varies from IC50 0.02 to 13,000 nM and is essentially the same for NTE-LysoPLA and NTE (r2 = 0.98). Third, the same six delayed toxicants administered i.p. to mice at multiple doses inhibit brain NTE-LysoPLA and NTE to the same extent (r2 = 0.90). Finally, their in vivo inhibition of brain NTE-LysoPLA generally correlates with delayed toxicity. Therefore...

Placental Failure and Impaired Vasculogenesis Result in Embryonic Lethality for Neuropathy Target Esterase-Deficient Mice

Moser, Markus; Li, Yong; Vaupel, Kristina; Kretzschmar, Doris; Kluge, Reinhart; Glynn, Paul; Buettner, Reinhard
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /02/2004 EN
Relevância na Pesquisa
17.89%
Age-dependent neurodegeneration resulting from widespread apoptosis of neurons and glia characterize the Drosophila Swiss Cheese (SWS) mutant. Neuropathy target esterase (NTE), the vertebrate homologue of SWS, reacts with organophosphates which initiate a syndrome of axonal degeneration. NTE is expressed in neurons and a variety of nonneuronal cell types in adults and fetal mice. To investigate the physiological functions of NTE, we inactivated its gene by targeted mutagenesis in embryonic stem cells. Heterozygous NTE+/− mice displayed a 50% reduction in NTE activity but underwent normal organ development. Complete inactivation of the NTE gene resulted in embryonic lethality, which became evident after gastrulation at embryonic day 9 postcoitum (E9). As early as E7.5, mutant embryos revealed growth retardation which did not reflect impaired cell proliferation but rather resulted from failed placental development; as a consequence, massive apoptosis within the developing embryo preceded its resorption. Histological analysis indicated that NTE is essential for the formation of the labyrinth layer and survival and differentiation of secondary giant cells. Additionally, impairment of vasculogenesis in the yolk sacs and embryos of null mutant conceptuses suggested that NTE is also required for normal blood vessel development.

Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration

Akassoglou, Katerina; Malester, Brian; Xu, Jixiang; Tessarollo, Lino; Rosenbluth, Jack; Chao, Moses V.
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
17.89%
Neuropathy target esterase (NTE) is a neuronal membrane protein originally identified for its property to be modified by organo-phosphates (OPs), which in humans cause neuropathy characterized by axonal degeneration. Drosophila mutants for the homolog gene of NTE, swisscheese (sws), indicated a possible involvement of sws in the regulation of axon-glial cell interaction during glial wrapping. However, the role of NTE/sws in mammalian brain pathophysiology remains unknown. To investigate NTE function in vivo, we used the cre/loxP site-specific recombination strategy to generate mice with a specific deletion of NTE in neuronal tissues. Here we show that loss of NTE leads to prominent neuronal pathology in the hippocampus and thalamus and also defects in the cerebellum. Absence of NTE resulted in disruption of the endoplasmic reticulum, vacuolation of nerve cell bodies, and abnormal reticular aggregates. Thus, these results identify a physiological role for NTE in the nervous system and indicate that a loss-of-function mechanism may contribute to neurodegenerative diseases characterized by vacuolation and neuronal loss.

Neuropathy target esterase catalyzes osmoprotective renal synthesis of glycerophosphocholine in response to high NaCl

Gallazzini, Morgan; Ferraris, Joan D.; Kunin, Margarita; Morris, Ryan G.; Burg, Maurice B.
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
18%
Glycerophosphocholine (GPC) is an osmoprotective compatible and counteracting organic osmolyte that accumulates in renal inner medullary cells in response to high NaCl and urea. We previously found that high NaCl increases GPC in renal [Madin–Darby canine kidney (MDCK)] cells. The GPC is derived from phosphatidylcholine, catalyzed by a phospholipase that was not identified at that time. Neuropathy target esterase (NTE) was recently shown to be a phospholipase B that catalyzes production of GPC from phosphatidylcholine. The purpose of the present study was to test whether NTE contributes to the high NaCl-induced increase of GPC synthesis in renal cells. We find that in mouse inner medullary collecting duct cells, high NaCl increases NTE mRNA within 8 h and NTE protein within 16 h. Diisopropyl fluorophosphate, which inhibits NTE esterase activity, reduces GPC accumulation, as does an siRNA that specifically reduces NTE protein abundance. The 20-h half-life of NTE mRNA is unaffected by high NaCl. TonEBP/OREBP is a transcription factor that is activated by high NaCl. Knockdown of TonEBP/OREBP by a specific siRNA inhibits the high NaCl-induced increase of NTE mRNA. Further, the lower renal inner medullary interstitial NaCl concentration that occurs chronically in ClCK1−/− mice and acutely in normal mice given furosemide is associated with lower NTE mRNA and protein. We conclude that high NaCl increases transcription of NTE...

Spongiform Pathology in Mouse CNS Lacking ‘Neuropathy Target Esterase’ and Cellular Prion Protein

Rosenbluth, Jack; Schiff, Rolf; Lam, Pokman; Nuriel, Tal; Chao, Moses V.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
17.89%
Conditional inactivation of the ‘neuropathy target esterase’ (NTE) gene in mouse nerve cells was previously shown to result in CNS pathology comparable to the spongiform encephalopathy characteristic of prion diseases. To determine whether cellular prion protein (PrPc) is essential for development of this pathology we examined hippocampi of mice lacking NTE alone, PrPc alone or both NTE and PrPc. Light microscopic survey showed clear-cut spongiform changes in a majority of NTE-/- and NTE/PrP-/- double knockout mice but in only one PrP-/- mouse. EM analysis of spongiform lesions from NTE-/- and NTE/PrP-/- mice, and from the one affected PrP-/- mouse, revealed patches of branching tubular inclusions, comparable to the ‘tubulovesicular inclusions’ described previously in prion diseases. We conclude that spongiform pathology in conditional NTE knockout mice is not mediated by PrPc, and that tubulovesicular inclusions can be seen in spongiform encephalopathy of other etiologies and are not pathognomonic of prion disease.

CREB Is Required for cAMP/PKA Signals Upregulating Neuropathy Target Esterase Expression

Chen, Jia-Xiang; Wu, Yi-Jun
Fonte: Mary Ann Liebert, Inc. Publicador: Mary Ann Liebert, Inc.
Tipo: Artigo de Revista Científica
Publicado em /04/2013 EN
Relevância na Pesquisa
18%
Neuropathy target esterase (NTE), which has been proposed as the primary target of organophosphorus compounds that cause delayed neuropathy with degeneration of nerve axons, is expressed primarily in neural cells but is also detected in non-neural cells. However, little is known about the regulation of NTE gene in cells. We found that a cyclic-AMP (cAMP)-response element (CRE) exists in the 5′ flanking sequence of NTE gene in HeLa cells, which implies that NTE may be regulated by the transcription factor cAMP-response element-binding protein (CREB). In the study, knockdown of CREB decreased the protein and mRNA levels of NTE and inhibited the upregulation by cAMP/PKA signaling. Moreover, we observed that knockdown of CREB significantly decreased luciferase activity of the NTE gene promoter, while it had no effect on that of the CREB binding sites of mutated NTE gene promoter and truncated NTE gene promoter lacking the CREB binding site. cAMP/PKA signals could increase NTE reporter gene activity, while knockdown of CREB inhibited the increase. We found that the transcription factor CREB can bind to the promoter sequence of NTE by chromatin immunoprecipitation. In conclusion, we provided evidence that CREB is required for cAMP/PKA signals upregulating NTE expression in HeLa cells.

Structural and Functional Characterization of the N Terminus of Schizosaccharomyces pombe Cwf10

Livesay, S. Brent; Collier, Scott E.; Bitton, Danny A.; Bähler, Jürg; Ohi, Melanie D.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /11/2013 EN
Relevância na Pesquisa
18.02%
The spliceosome is a dynamic macromolecular machine that catalyzes the removal of introns from pre-mRNA, yielding mature message. Schizosaccharomyces pombe Cwf10 (homolog of Saccharomyces cerevisiae Snu114 and human U5-116K), an integral member of the U5 snRNP, is a GTPase that has multiple roles within the splicing cycle. Cwf10/Snu114 family members are highly homologous to eukaryotic translation elongation factor EF2, and they contain a conserved N-terminal extension (NTE) to the EF2-like portion, predicted to be an intrinsically unfolded domain. Using S. pombe as a model system, we show that the NTE is not essential, but cells lacking this domain are defective in pre-mRNA splicing. Genetic interactions between cwf10-ΔNTE and other pre-mRNA splicing mutants are consistent with a role for the NTE in spliceosome activation and second-step catalysis. Characterization of Cwf10-NTE by various biophysical techniques shows that in solution the NTE contains regions of both structure and disorder. The first 23 highly conserved amino acids of the NTE are essential for its role in splicing but when overexpressed are not sufficient to restore pre-mRNA splicing to wild-type levels in cwf10-ΔNTE cells. When the entire NTE is overexpressed in the cwf10-ΔNTE background...

Neuropathy Target Esterase Is Required for Adult Vertebrate Axon Maintenance

Read, David J.; Li, Yong; Chao, Moses V.; Cavanagh, John B.; Glynn, Paul
Fonte: Society for Neuroscience Publicador: Society for Neuroscience
Tipo: Artigo de Revista Científica
Publicado em 16/09/2009 EN
Relevância na Pesquisa
17.89%
The enzyme neuropathy target esterase (NTE) is present in neurons and deacylates the major membrane phospholipid, phosphatidylcholine (PtdCho). Mutation of the NTE gene or poisoning by neuropathic organophosphates—chemical inhibitors of NTE—causes distal degeneration of long spinal axons in humans. However, analogous neuropathological changes have not been reported in nestin-cre:NTEfl/fl mice with NTE-deficient neural tissue. Furthermore, altered PtdCho homeostasis has not been detected in NTE-deficient vertebrates. Here, we describe distal degeneration of the longest spinal axons in ∼3-week-old nestin-cre:NTEfl/fl mice and in adult C57BL/6J mice after acute dosing with a neuropathic organophosphate: in both groups early degenerative lesions were followed by swellings comprising accumulated axoplasmic material. In mice dosed acutely with organophosphate, maximal numbers of lesions, in the longest spinal sensory axon tract, were attained within days and were preceded by a transient rise in neural PtdCho. In nestin-cre:NTEfl/fl mice, sustained elevation of PtdCho over many months was accompanied by progressive degeneration and massive swelling of axons in sensory and motor spinal tracts and by increasing hindlimb dysfunction. Axonal lesion distribution closely resembled that in hereditary spastic paraplegia (HSP). The importance of defective membrane trafficking in HSP and the association of NTE with the endoplasmic reticulum—the starting point for the constitutive secretory pathway and transport of neuronal materials into axons—prompted investigation for a role of NTE in secretion. Cultured NTE-deficient neurons displayed modestly impaired secretion...

Organophosphate-Induced Changes in the PKA Regulatory Function of Swiss Cheese/NTE Lead to Behavioral Deficits and Neurodegeneration

Wentzell, Jill S.; Cassar, Marlène; Kretzschmar, Doris
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 18/02/2014 EN
Relevância na Pesquisa
27.68%
Organophosphate-induced delayed neuropathy (OPIDN) is a Wallerian-type axonopathy that occurs weeks after exposure to certain organophosphates (OPs). OPs have been shown to bind to Neuropathy Target Esterase (NTE), thereby inhibiting its enzymatic activity. However, only OPs that also induce the so-called aging reaction cause OPIDN. This reaction results in the release and possible transfer of a side group from the bound OP to NTE and it has been suggested that this induces an unknown toxic function of NTE. To further investigate the mechanisms of aging OPs, we used Drosophila, which expresses a functionally conserved orthologue of NTE named Swiss Cheese (SWS). Treating flies with the organophosporous compound tri-ortho-cresyl phosphate (TOCP) resulted in behavioral deficits and neurodegeneration two weeks after exposure, symptoms similar to the delayed effects observed in other models. In addition, we found that primary neurons showed signs of axonal degeneration within an hour after treatment. Surprisingly, increasing the levels of SWS, and thereby its enzymatic activity after exposure, did not ameliorate these phenotypes. In contrast, reducing SWS levels protected from TOCP-induced degeneration and behavioral deficits but did not affect the axonopathy observed in cell culture. Besides its enzymatic activity as a phospholipase...

Motor neuron disease due to neuropathy target esterase mutation: enzyme analysis of fibroblasts from human subjects yields insights into pathogenesis

Hein, Nichole D.; Rainier, Shirley R.; Richardson, Rudy J.; Fink, John K.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
18.13%
Recently, we identified neuropathy target esterase (NTE) mutation as the cause of an autosomal recessive motor neuron disease (NTE-MND). Subsequently, we showed that NTE-MND mutations reduced specific activity (SA) and altered inhibitory kinetics of NTE catalytic domain constructs. Recent preliminary results showed that NTE is expressed in cultured human skin fibroblasts, and others have used mutant forms of neuronal proteins expressed in fibroblasts as biomarkers of neurogenetic diseases. Therefore, the present study was carried out to test the hypothesis that NTE in cultured skin fibroblasts from NTE-MND subjects also exhibit altered enzymological properties assessed by SA and IC50 values of mipafox (MIP) and chlorpyrifos oxon (CPO). NTE SA was reduced to 65% of control (wild type NTE from commercially obtained fibroblasts) in homozygous M1012V fibroblasts and 59-61% of control in compound heterozygous R890H/c2946_2947InsCAGC fibroblasts. MIP IC50 values were unaffected by the NTE mutations, but the CPO IC50 increased 4.5-fold in homozygous M1012V fibroblasts. Interestingly, markedly reduced NTE SAs (40-43% of control) were observed in fibroblasts from asymptomatic subjects heterozygous for NTE insertion c2946_2947InsCAGC. This insertion is predicted to produce truncated NTE missing the last 235 residues of its catalytic domain. These observations confirm that NTE-MND mutations reduce NTE SA in vitro. Moreover...

Entre o discurso modernizante e a precariedade da prática: Núcleo de Tecnologia Educacional e formação de professores; Between the discourse of modernization and the precariousness of Practice: Center for Educational Technology and Teacher Education

Santos, Sebastião Pereira dos
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Educação; Ciências Humanas Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Educação; Ciências Humanas
Tipo: Dissertação Formato: application/pdf
POR
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This study, in the research approach of teachers formation and professionalization of the Post Graduation Program of Education College of Universidade Federal de Goiás (UFG), had as objectives to investigate the quality of the multipliers teachers formation process at the Núcleo de Tecnologia Educacional (NTE) Goiânia regarding to the critical reflexive reference for the use of the Technologies of Information and Communication (TICs) and to analyse the real conditions related to that formation process. Some of the questions which directed this study development are: how does the public teachers formation for the use of the TICs developed by NTE-Goiânia multipliers teachers happen? The courses offered by NTE Goiânia are based in which conceptions? Do the courses offer anything besides technical training? To answer these and other questions which appeared during the research, the Study of case was used in a historical dialectical perspective. In order to have a theoretical base we searched the contribution of Barreto (2001; 2004 and 2006), Belloni (2001; 2005), Castells (1996), Cysneiros (1999; 2000 and 2001), Dupas (2003 and 2000), Frigotto (1996, 2001), Kenski (2001; 2003), Pretto (2001; 2002 and 2006), Santos (2003)...

Silencing of PNPLA6, the Neuropathy Target Esterase (NTE) codifying gene, alters neurodifferentiation of human embryonal carcinoma stem cells (NT2)

PAMIES David; PRICE Anna; FABBRI Marco; GRIBALDO Laura; SCELFO BIBIANA; HARRIS GEORGINA; COLLOTTA Angelo; VILANOVA Eugenio; SOGORB Miguel A.
Fonte: PERGAMON-ELSEVIER SCIENCE LTD Publicador: PERGAMON-ELSEVIER SCIENCE LTD
Tipo: Articles in Journals Formato: CD-ROM
ENG
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Neuropathy Target Esterase (NTE) is a protein involved in the development of a polyneuropathy caused by exposure to certain organophosphorus compounds. In vivo and in vitro studies have also associated NTE with embryonic development since NTE null mice embryos are non-viable, and silencing the NTE-codifying gene (Pnpla6) in mouse embryonic stem cells strongly alters the differentiation of vascular and nervous systems. In this paper, human embryonal carcinoma stem cells (hNT2) are used as an in vitro neurodifferentiation model to determine whether PNPLA6 silencing is able to alter the differentiation process. In control cultures, PNPLA6 mRNA levels increased in parallel with other neuroectodermal markers during neurodifferentiation. PNPLA6 silencing with specific interference RNA reached a 97% decrease in gene expression 3 days after transfection and with a maximum reduction in NTE enzymatic activity (50%), observed on day 4. Silencing PNPLA6 showed an 80% decrease in quantifiable neuronal cells after 13 days in vitro (DIV) compared to controls and absence of different neuronal markers after 66 DIV. Microarray data analysis of the PNPLA6-silenced cells showed alterations in several developmental processes, mainly neurogenesis and epithelium tube morphogenesis. PNPLA6 silencing also led to a reduction in electrical activity and an altered neuronal phenotype. This work is the first proof supporting the hypothesis that NTE plays a role in human early neurodevelopment using a human cell differentiation model.; JRC.I.5-Systems Toxicology

Redonda me(nte) amarela

Tibola, Talita
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
27.08%
Entre o estilo de Gilles Deleuze e o prazer do texto de Roland Barthes: o texto. Uma escuta das palavras e das coisas, a escritura em primeiro plano e tudo o que ela desestabiliza: o pensamento, o corpo, uma cor sem-lugar. Tirar as palavras de seu lugar, trabalhar com recortes de frases: um procedimento. O duplo, o monstro, o Corpo sem Órgãos, a morte, o esvaecimento, o sentido, o acontecimento. Uma obsessão, sutil talvez: o amarelo, o amarelo-acontecimento. Repetição: o amarelo insistência, persistência. Palavra, som, coisa, efeito. De poesia? O texto: fala do amarelo. Mas, antes, do que desaparece e se torna transparente. Do corpo imóvel, de sua dura queda e de seu esvair-se. O que há depois do esquecimento? Talvez o amarelo. Um corpo desarrumado? De um corpo desfeito, o que resta é o intenso. Amarelo intenso. Amar-elo. Do amar, o elo. Da escrita, a marca. Redonda-me(nte)-amarela.; Entre le style de Gilles Deleuze et le plaisir du texte de Roland Barthes: le texte. Une écoute de mots et de choses. L'écriture surtout et tout ce qu'elle dérange: la pensée, le corps, une couleur sans lieu. Déplacer les mots, découper les énoncés: un procédé. Le double, le monstre, le Corps sans Organe, la mort, la dissipation, le sens...

Enexinnova Creación de Valor a Través de Innovación en Enex

Distribuidora y Comercializacion de Accesorios Ycombustibles Nte Ltda; Empresa Nacional de Energía Enex S. A.; Servicios e Inversiones Lolenco Limitada; Javiera Francisca Sarmiento Martinez
Fonte: Corporação de Fomento da Produção Publicador: Corporação de Fomento da Produção
Tipo: proyecto
Publicado em 26/06/2012
Relevância na Pesquisa
27.08%
Enex es una Compañía en Constantes Cambios a Partir de su Formación en 2011 con la Compra de los Activos de Shell Chile por el Grupo Quiñenco. Desde Ese Instante la Estrategia de la Compañía Considero que la Innovación y la Creatividad Son Actitudes Claves que Necesitan Ser Compartidas por la Organización con Miras hacia el Logro de los Objetivos Centrales en los Próximos Años de Crecimiento Orgánico Sustentabilidad y Excelencia Operacional. A Raíz de esto la Compañía ha Definido que la Innovación Sea una de las Herramientas Más Importantes y Poderosas para que Enex se Convierta en un Actor Relevante en el Mercado Energético Nacional. Con la Mirada Puesta en el Cliente Tanto Consumidores como Industrias se Puso en Marcha el Programa de Innovación de la Compañía que Aspira a Generar un Proceso Establecido y Sistemático para la Generación de Nuevas Ideas su Evaluación y Posterior Implementación en Temas Definidos como Nuevas Propuestas de Valor Mejoras en la Operación y en el Relacionamiento con los Clientes Socios y la Comunidad en General. Este Programa en Funcionamiento desde Abril de 2012 Tiene por Objetivo Establecer Procesos para Innovar como También Posibilitar el Desarrollo de Competencias para la Innovación en la Organización a Través de Intervenciones en la Cultura de la Compañía. el Programa que Está Sido Delineado y Ejecutado con Apoyo de la Consultora Transforme se Organiza en Torno a Desafíos de Ideas desde los Cuales Surgirán Propuestas de Proyectos que Serán Evaluados e Implementados al Mismo Tiempo se Busca Incentivar el Surgimiento de Competencias y Actitudes Propicias para la Innovación en la Organización a Través de Sucesivas Actividades de Formación y Motivación. La Compañía Presenta Esta Postulación con Miras a Profundizar y Ampliar el Alcance de su Actual Programa de Innovación para que a Partir de los Recursos Obtenidos a Través del Subsidio Corfo para Gestión de la Innovación en Empresas 2012 se Pueda Incorporar por un Lado su Filial: Dicomac y por Otro Lado Realizar un Mayor Número de Actividades de Capacitación y Motivación Ampliando Así la Cobertura y Alcance del Programa Dentro de la Compañía lo que También Incluye Incrementar su Plazo de Duración Original. De Esta Forma el Programa de Innovación Espera Lograr en 18 Meses de Ejecución (contando desde Abril de 2012 Fecha de su Lanzamiento) Resultados en Surgimiento de Proyectos Innovadores Concretos para Ser Implementados Cambios Culturales en la Organización y Posicionamiento de Enex como Compañía Innovadora Todo Gracias a un Modelo de Innovación Abierta que Combina las Importantes Capacidades Internas Adquiridas tras los Largos Años de Operación del Negocio y Capacidades Externas Entregadas por la Consultoría Experta y el Relacionamiento con Entes Innovadores como Universidades Centros Tecnológicos Emprendimientos entre Otros. el Programa Pretende Transformarse en un Vehículo para Unir y Mejorar la Comunicación Dentro de la Organización al Incorporar También a la Filial Dicomac en Todas sus Actividades y Asimismo a las Instalaciones en Diversas Partes del País como Antofagasta y Concepción que También se Sumarán al Programa Participando de sus Convocatorias Junto al Vital Apoyo de nuestros Clientes y Proveedores Todo en Pro de Hacer de la Innovación Nuestra Mayor Herramienta de Creación de Valor.; Consolidar la Estrategia de Innovación de la Compañía y la Estructura Organizacional Propuesta Incluyendo el Comité de Innovación de Forma Tal que la Organización Reconozca a Esta Estructura como Apoyo y Catalizador del Proceso de Innovación.; Detectar las Capacidades para Innovar Dentro de la Compañía a Través de los Distintos Desafíos e Iniciativas que Surgen de ellos Identificando Asimismo Oportunidades para Colaborar con Entidades Externas que Apoyen a Enex en Llevar a Cabo Estas Iniciativas.; Enexinnova Tiene por Objetivo Principal Configurar el Ecosistema de Innovación en la Compañía a Través de la Creación y Fortalecimiento de la Estructura y Cultura Organizacional lo que Permitirá la Generación de Procesos para Innovar en Forma Sistemática y Organizada Permitiendo el Surgimiento Constante de Nuevas Ideas los que Transformados en Proyectos Generarán Soluciones que se Espera Puedan Ser Implementadas durante el Tiempo de Ejecución del Programa. Estas Iniciativas se Gestarán con el Propósito de Favorecer al Negocio en su Conjunto con Nuevas Propuestas de Valor Formas de Hacer y Mejoras de Operación. Para la Sustentabilidad del Programa es Vital el Surgimiento de Innovadores Naturales en la Compañía por lo que los Procesos para este Fin También Forman Parte del Objetivo Principal. Se Espera que Estos Innovadores con las Herramientas que Les Otorgue el Programa Puedan Liderar la Implementación de sus Ideas Haciéndose Parte de las Mejoras que Éstas Generarán para Enex.; Establecer Indicadores para Medir la Actividad de Innovación de la Compañía.; Establecer Procesos para Innovar en Forma Sistemática y Constante en la Compañía en las Dimensiones de Propuesta de Desafíos de Innovación Generación de Ideas Evaluación de Propuestas e Implementación de Proyectos que Puedan Ser Utilizados por la Organización a Través del Tiempo. Esto Incluye Establecer Responsables y Capacidades para los Diversos Roles y Generar un Pipeline de Proyectos que Evolucionará en el Tiempo.; Influenciar el Clima Organizacional para Propiciar Cambios Culturales que Hagan de Enex una Compañía Más Permeable y Dispuesta a Reconocer Oportunidades para Innovar en Todos sus Niveles Creando Valor a Clientes Socios y la Propia Compañía.; Potenciar y Ampliar el Actual Programa de Gestión de Innovación que se Lleva a Cabo en la Compañía a Partir de los Recursos Adicionales Provenientes del Subsidio Entregado por Corfo para este Fin.; Propiciar el Surgimiento Espontáneo de Innovadores Internos que Lideren la Implementación de Ideas que Han Sido Transformadas en Proyectos de Innovación Luego del Proceso de Evaluación.; Corporación de Fomento de la Producción

Common origin of exotic properties in ceramic and hybrid negative thermal expansion materials

Fang, Hong; Dove, Martin T.; Phillips, Anthony E.
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
17.89%
Many ceramic and hybrid metal-organic framework materials show negative thermal expansion (NTE): they \textit{contract} instead of expanding on heating \cite{Barrera_Miller_Lind_Romao 2005}. Their structures are invariably characterised as a network of polyhedral groups of atoms that are connected through sharing of corner atoms or by shared ligands. Empirically, NTE materials tend to show pressure-induced softening, pressure enhancement of NTE, and the reduction of NTE on heating. But such effects have only been investigated in a small number of materials \cite{Pantea 2006,Chapman 2005,Chapman 2007,Fangexp 2013}, and as yet there is no general framework for understanding the whole suite of properties together. By studying models with Hamiltonians chosen to reflect the physical picture generally accepted as responsible for NTE in framework materials, we demonstrate that NTE, pressure-enhanced NTE, and pressure-induced softening naturally emerge together. We then show how anharmonic interactions lead to structural warm hardening---something that has only previously been seen in laser-excited warm-dense matter \cite{Ernstorfer 2009}---as well as to the transition from NTE to positive thermal expansion and the disappearing of the pressure-induced softening at high temperatures.