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Mycobiota and aflatoxigenic profile of Portuguese almonds and chestnuts from production to commercialisation

Rodrigues, Paula
Fonte: Instituto Politécnico de Bragança Publicador: Instituto Politécnico de Bragança
Tipo: Tese de Doutorado
ENG
Relevância na Pesquisa
27.53%
Aflatoxin (AF) contamination of nuts is an increasing concern to the consumer’s health. Portugal is a big producer of almonds and chestnuts, but there is no scientific knowledge on the safety of those nuts. AFs B1, B2, G1 and G2 are produced mainly by some species of Aspergillus belonging to section Flavi, which is composed of a large number of very closely related species. While these species are difficult to differentiate morphologically and even genetically, they differ in a characteristic that is of paramount importance for food safety, as only some are responsible for the production of the highly toxigenic AFs. Taxonomy and species identification are therefore subject of great interest, and the establishment of schemes for species and for aflatoxigenic strains identification that are simultaneously accurate, sensitive, robust and expedite is mandatory. This work had three major goals: the first was to provide knowledge on the general mycobiota, aflatoxigenic fungi and AF contamination of Portuguese almonds and chestnuts, and its evolution throughout the various stages of production (field, storage and processing). For this matter, 45 chestnut samples were collected from orchards from Trás-os-Montes. Forty-seven almond samples were collected in Trás-os-Montes at different stages of production: field...

Mycobiota and mycotoxins of almonds and chestnuts with special reference to aflatoxins

Rodrigues, Paula; Venâncio, Armando; Lima, Nelson
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
27.21%
Almond tree, Prunus dulcis, is a cultivated tree originating from wild trees from Central Asia which is currently dispersed throughout the world, mainly in Mediterranean countries and others with similar climatic characteristics, like USA. Chestnuts are produced by a wide variety of species from the genus Castanea. These species divide into three major groups that have specific geographical distribution and produce three varieties of chestnuts: the Asian (Japanese and Chinese) chestnut; the American chestnut; and the European (sweet) chestnut. Studies on mycobiota of almonds and chestnuts make it clear that Aspergillus species are frequent in these commodities, being Sections Flavi and Nigri the predominant aspergilla. Despite of it, the contamination risk with Aspergillus Section Flavi and their mycotoxins, namely aflatoxins, is much more studied than any other. Tree nuts are among the commodities with moderate to high risk of aflatoxin contamination, since they are generally produced under environmental conditions which also favour growth of aflatoxigenic fungi and toxin production. In fact, biotic and abiotic conditions under which nuts are produced, stored and processed are key factors to the level of fungal and aflatoxin contamination. The aim of this review is to outline the overall aspects underlying fungal and mycotoxin contamination of two of the most important nuts produced worldwide...

Mycobiota and mycotoxins in Brazil nut samples from different states of the Brazilian Amazon region

Reis, T. A.; Oliveira, T. D.; Baquião, A. C.; Goncalves, S. S.; Zorzete, P.; Correa, B.
Fonte: ELSEVIER SCIENCE BV; AMSTERDAM Publicador: ELSEVIER SCIENCE BV; AMSTERDAM
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
27.21%
The objective of this study was to evaluate the presence of fungi and mycotoxins (aflatoxins and cyclopiazonic acid) in Brazil nut samples collected in different states of the Brazilian Amazon region: Acre, Amazonas, Amapa, and Para. A total of 200 husk samples and 200 almond samples were inoculated onto Aspergillus flavus-parasiticus agar for the detection of fungi. Mycotoxins were analyzed by high-performance liquid chromatography. The mycobiota comprised the following fungi, in decreasing order of frequency: almonds - Phialemonium spp. (54%), Penicillium spp. (16%), Fusarium spp. (13%), Phaeoacremonium spp. (11%), and Aspergillus spp. (4%), husks - Phialemonium spp. (62%), Phaeoacremonium spp. (11%), Penicillium spp. (10%), Fusarium spp. (9%), and Aspergillus spp. A polyphasic approach was used for identification of Aspergillus species. Aflatoxins were detected in 22 (11%) of the 200 almond samples, with 21 samples presenting aflatoxin B-1 levels above 8 mu g/kg, the limit established by the European Commission for Brazil nuts for further processing. Nineteen (9.5%) of the 200 husk samples contained aflatoxins, but at levels lower than those seen in almonds. Cyclopiazonic acid (CPA) was detected in 44 (22%) almond samples, with levels ranging from 98.65 to 1612 mu g/kg. Aspergillus nomius and A. flavus were the most frequent Aspergillus species. The presence of fungi does not necessarily imply mycotoxin contamination...

Mycobiota, aflatoxins and cyclopiazonic acid in stored peanut cultivars

Zorzete, Patricia; Baquião, Arianne Costa; Atayde, Danielle Diniz; Reis, Tatiana Alves dos; Gonçalez, Edlayne; Correa, Benedito
Fonte: SciVerse ScienceDirect; Essex Publicador: SciVerse ScienceDirect; Essex
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
27.21%
This study evaluated the presence of fungi and mycotoxins [aflatoxins (AFs), cyclopiazonic acid (CPA), and aspergillic acid] in stored samples of peanut cultivar Runner IAC Caiapó and cultivar Runner IAC 886 during 6 months. A total of 70 pod and 70 kernel samples were directly seeded onto Aspergillus flavus and Aspergillus parasiticus agar for fungi isolation and aspergillic acid detection, and AFs and CPA were analyzed by high-performance liquid chromatography. The results showed the predominance of Aspergillus section Flavi strains, Aspergillus section Nigri strains, Fusarium spp., Penicillium spp. and Rhizopus spp. from both peanut cultivars. AFs were detected in 11.4% of kernel samples of the two cultivars and in 5.7% and 8.6% of pod samples of the Caiapó and 886 cultivars, respectively. CPA was detected in 60.0% and 74.3% of kernel samples of the Caiapó and 886 cultivars, respectively. Co-occurrence of both mycotoxins was observed in 11.4% of kernel samples of the two cultivars. These results indicate a potential risk of aflatoxin production if good storage practices are not applied. In addition, the large number of samples contaminated with CPA and the simultaneous detection of AFs and CPA highlight the need to investigate factors related to the control and co-occurrence of these toxins in peanuts.; FAPESP; CNPq; ANVISA; Cooperativa Agrícola Mista da Alta Paulista (CAMAP)

Micobiota e ocorrência de fumonisinas em diferentes genótipos de milho transgênico (Bt).; Mycobiota and occurrence of fumonisins in different genotypes of transgenic corn (Bt).

Barroso, Vinicius de Morais
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 15/04/2013 PT
Relevância na Pesquisa
27.53%
Fusarium verticillioides, produtor de fumonisinas, é comumente associado aos grãos de milho. O objetivo proposto foi pesquisar a micobiota e fumonisinas em diferentes genótipos de milho transgênico (Bt) e seus respectivos controles. A micobiota dos grãos, realizada pela técnica da semeadura direta, revelou a presença de variados gêneros fúngicos. As cepas de Fusarium isoladas foram identificadas até espécie por metodologia clássica e molecular. A atividade de água média nos híbridos foi superior a 0,90. Observamos maior contaminação fúngica nos controles, com exceção do 2B710Hx. A freqüência relativa do gênero Fusarium nos diferentes híbridos revelou menor contaminação nos transgênicos, excetuando o AG8088 VTPRO. F. verticillioides foi o fungo mais frequente. A análise de fumonisinas, revelou fumonisina B1 em 92,5 % das amostras e fumonisina B2 em 47,1%. Não foi constatada variação na produção de fumonisinas nos genótipos 2B710 e 30F35 (transgênico e não transgênico). Já para o genótipo AG8088 houve menor produção de fumonisina B1 na variedade transgênica.; Fusarium verticillioides, fumonisin producer, is commonly associated with corn kernels. The proposed objective was to investigate the mycobiota and fumonisins in different genotypes of transgenic corn (Bt) and their respective controls. The mycobiota of grain...

Sistemas de rotação de culturas e infecção de grãos de milho por Fusarium verticillioides em regiões produtoras no estado de São Paulo.; Crop rotation systems and infection of maize grains by Fusarium verticillioides in maize-producing regions in the state of São Paulo.

Atayde, Danielle Diniz
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 20/02/2014 PT
Relevância na Pesquisa
27.21%
Propomos avaliar a influência da rotação de culturas na infecção de milho transgênico (Bt) e cobertura morta por F. verticillioides e na presença de fumonisinas em amostras provenientes de Palmital e Capão Bonito. O isolamento fúngico da cobertura morta e do milho foi realizado em DG18 e DRBC, respectivamente. Os fungos isolados pertencentes ao gênero Fusarium foram identificados até espécie. Utilizamos, para análise de fumonisinas, Cromatografia Líquida de Alta Eficiência. Micobiota da cobertura morta revelou maior frequência de Cladosporium spp. Nas amostras de milho, F. verticillioides foi o fungo mais isolado. Dentro do gênero Fusarium, a espécie F. verticillioides foi a mais frequente. Análise micotoxicológica do milho revelou a presença de fumonisinas em 88,9% das amostras provenientes de Palmital e em 86,1% de Capão Bonito. Dos isolados de F. verticillioides 84,6% foram produtores de FB1 + FB2. A presença de F. verticillioides e fumonisinas nos grãos de milho estabelece um problema econômico e de saúde pública.; The objective of this study was to evaluate the influence of crop rotation systems on the infection of maize grains and mulch by F. verticillioides and on the presence of fumonisins in maize samples collected in Palmital and Capão Bonito. Surface seeding (DG18) and direct seeding (DRBC) were employed for fungal isolations from mulch and maize...

Micobiota e ocorrência de micotoxinas em amostras de castanha-do-Brasil provenientes de diferentes estados brasileiros.; Mycobiota and mycotoxins in Brazil nut samples from different Brazilian states.

Reis, Tatiana Alves dos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 24/02/2014 PT
Relevância na Pesquisa
27.21%
O objetivo deste estudo foi avaliar a presença de fungos e micotoxinas [aflatoxinas e ácido ciclopiazônico (ACP)] em amostras de castanha-do-Brasil coletadas em diferentes estados da região Amazônia brasileira: Acre, Amazonas, Amapá e Pará. Um total de 200 amostras de casca e 200 amostras de amêndoa foram semeadas em ágar Aspergillus flavus-parasiticus para pesquisa da micobiota. As micotoxinas foram analisadas por cromatografia líquida de alta eficiência. A morfológica clássica e a análise molecular revelou a presença dos seguintes fungos, em ordem decrescente de frequência: Phialemonium spp., Penicillium spp., Fusarium spp., Phaeoacremonium spp. e Aspergillus spp. A presença de aflatoxinas e ACP foi detectada em amostras de amêndoa, na frequência de 11% e 22%, respectivamente. Aspergillus nomius e Aspergillus flavus foram as espécies mais isoladas de Aspergillus. A presença de fungos não implica necessariamente na contaminação por micotoxinas, porém a amêndoa da castanha-do-Brasil parece ser um bom substrato para o crescimento de fungos.; The aim of this study was to evaluate the presence of fungi and mycotoxins [aflatoxin and cyclopiazonic acid (ACP)] in Brazil nut samples collected in different States of the Brazilian Amazon: Acre ...

Avaliação da micobiota de grãos de café e dos metabolitos fungicos na qualidade da bebida; Evaluation of coffe bean mycobiota and fungal metabolites on beverage quality

Beatriz Thie Iamanaka
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 27/01/2010 PT
Relevância na Pesquisa
27.53%
O café passa por vários processos até chegar a ser consumido como bebida e vários fatores contribuem para a sua qualidade final, dentre eles a população microbiana presente. A contaminação dos grãos pelos microrgranismos é diversificada, envolvendo a participação de bactérias, bolores e leveduras, com a predominância de um ou outro grupo, dependendo da etapa de processamento dos grãos. Existem evidências, ainda não conclusivas de que vários fungos presentes no café podem produzir uma série de compostos que podem vir a prejudicar a qualidade da bebida. Esta pesquisa teve como objetivos analisar a micobiota dos grãos obtidos em diferentes etapas da cadeia produtiva do café; investigar a produção dos compostos voláteis produzidos pelos isolados e o impacto dos mesmos na qualidade da bebida e; avaliar sensorialmente a bebida, correlacionando com os fungos presentes. A micobiota de 41 amostras de grãos de café cru, de duas regiões produtoras do Brasil, Cerrado Mineiro/MG e Piraju/SP foram analisadas. As amostras foram coletados do pé (cereja), do solo (varreção), do terreiro (maduro, seco e passas no pé e verde) e da tulha (estocagem) e comparados dois tipos de preparo dos grãos: secagem natural e cereja descascado. As amostras de Minas Gerais apresentaram baixa infecção fúngica...

Application of classification-tree models to characterize the mycobiota of grapes on the basis of origin

Serra, Rita; Lourenço, Anália; Belo, Orlando; Venâncio, Armando
Fonte: Asociación Española de Especialistas en Micología Publicador: Asociación Española de Especialistas en Micología
Tipo: Artigo de Revista Científica
Publicado em //2006 ENG
Relevância na Pesquisa
37.72%
Classification-tree(CT) models are a simple and robust exploratory data analysis technique that can be used in classification, regressions and summaries of data. They distill complex ecological relationships into simplified rules and identify the species necessary for sample classification on the basis of detailed ecological inventories. The usefulness of this technique to characterize and represent differences in the grape mycobiota of distinct origins was evaluated. Grapes from four Portuguese winemaking regions were selected for a 3-year study: Alentejo, Douro, Ribatejo and Vinhos Verdes. The mycobiota of grapes was assessed with planting methods and the frequencies of isolations of the fungal taxa identified in 32 samples were used as a training dataset. The CT algorithm selected the fungal taxa and respective frequencies to classify grapes according to its region of origin. The ten-fold cross-validation technique was used for model evaluation. The success rate of the model was quantified and expressed in the number of correctly classified samples overall and into region. Furthermore, model refinement was performed using attribute selection algorithms and class redefinition. A simple tree model was generated that classified grapes into three regional origins: Douro...

Mycobiota and aflatoxigenic profile of Portuguese almonds and chestnuts from production to commercialisation

Rodrigues, Paula Cristina Azevedo
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Tese de Doutorado
Publicado em 01/03/2011 ENG
Relevância na Pesquisa
27.53%
Tese de doutoramento em Engenharia Química e Biológica; Aflatoxin (AF) contamination of nuts is an increasing concern to the consumer’s health. Portugal is a big producer of almonds and chestnuts, but there is no scientific knowledge on the safety of those nuts. AFs B1, B2, G1 and G2 are produced mainly by some species of Aspergillus belonging to section Flavi, which is composed of a large number of very closely related species. While these species are difficult to differentiate morphologically and even genetically, they differ in a characteristic that is of paramount importance for food safety, as only some are responsible for the production of the highly toxigenic AFs. Taxonomy and species identification are therefore subject of great interest, and the establishment of schemes for species and for aflatoxigenic strains identification that are simultaneously accurate, sensitive, robust and expedite is mandatory. This work had three major goals: the first was to provide knowledge on the general mycobiota, aflatoxigenic fungi and AF contamination of Portuguese almonds and chestnuts, and its evolution throughout the various stages of production (field, storage and processing). For this matter, 45 chestnut samples were collected from orchards from Trás-os-Montes. Forty-seven almond samples were collected in Trás-os-Montes at different stages of production: field...

Mycotoxins and mycobiota in Brazil nuts and strategies for their control

Silva, Otniel Freitas
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Tese de Doutorado
Publicado em 16/12/2011 ENG
Relevância na Pesquisa
27.53%
Tese de doutoramento em Engenharia Biológica e Química; The Brazil nut (Bertholethia excelsa) is an important non timber forest product (NTFP) from the Amazonian forest. Despite their nutritious value, Brazil nuts are susceptible to fungi contamination. The mycobiota associated in nuts is mainly concern due to some species of Aspergillus belonging to section Flavi, which is composed of a large number of very closely related species, same of which may produce Aflatoxins (AF). AF presence in nuts is not only an increasing concern to the consumer’s health but also to economic aspects, causing losses in all phases of global distribution chain, affecting major exporting countries. The purpose of this study was to determine which part of the nut contributes to contamination by aflatoxins and to identify the mycobiota in Brazil nut samples. Unshelled and shell nuts were analyzed by measuring the total count of filamentous fungi (Aspergillus sections Flavi, Nigri and Circumdati) in sanitised (1 % sodium hypochlorite for 10 minutes) and non-sanitised treatments. To perform the AF analysis, samples of Brazil nuts were milled separately. The AF from the shell and kernel were extracted with chloroform and analysed by the HPLC-FD system in isocratic mode. The Aspergillus section Flavi count was 21.67% lower and the production of AF by this section was around 25%. The AFB1 concentrations of shelled nuts and shell samples were 35.0 and 1.78 μg/kg...

Ácido D-glucônico e sua relação com a micobiota de uvas produzidas em Santa Catarina; D-gluconic acid and its relation with the mycobiota of grapes produced in Santa Catarina

Nunes, Estela de Oliveira; Furigo Junior, Agenor; Venâncio, Armando
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Conferência ou Objeto de Conferência
Publicado em 06/08/2008 POR
Relevância na Pesquisa
27.72%
D-gluconic acid concentration in musts and wines is natural of acid sources, being produced by filamentous fungi and/or bacteria. Aspergillus, Botrytis, and Penicillium sp., oxidize to glucose to produce D-gluconic acid, that is not used by yeasts or bacteria and can be used as a fruit deterioration indicator. Since the detection of ochratoxin A (OTA) in juices and wines related studies to mycobiota of the grapes, under the ochratoxigenic potential point of view has been being evaluated. The present study had the goal of evaluating the grapes sanity degree produced in Santa Catarina in the crops 2005/2006, using the D-gluconic acid concentration as indicator. D-gluconic acid correlation between OTA, mycobiota and population of Aggregate A. niger went 0,017; 0,004 and 0,008, respectively, reflecting the low correlation presented with OTA. For the population of Botrytis the decisive factor was 0751, or only 24.9% of the variation can not be explained, since the mycobiota with the relationship was very low, reinforcing the hypothesis of association with other factors such as the presence of bitter rot.

Caracterização preliminar do micobiota de enchidos tradicionais portugueses embalados em atmosferas protectoras

Almeida, Inês Filipa Martins de
Fonte: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária Publicador: Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária
Tipo: Trabalho de Conclusão de Curso
Publicado em 21/09/2009 POR
Relevância na Pesquisa
27.53%
Dissertação de Mestrado Integrado em Medicina Veterinária; Os produtos cárneos tradicionais (enchidos) apresentam uma grande variedade comercial, como resultado da diversidade das matérias-primas, dos ingredientes e dos processos de fabrico utilizados. A qualidade do produto pode estar em causa sempre que se desenvolvem microrganismos que comprometam o sabor, o cheiro, a consistência e o aspecto destes produtos, ou seja, microrganismos envolvidos em processos glucolíticos, proteolíticos e lipolíticos. A presença de leveduras lipolíticas pode afectar a qualidade destes géneros alimentícios. A β-oxidação peroxissomal das leveduras resulta na produção de peróxido de hidrogénio e ácidos gordos livres pela degradação incompleta dos triacilgliceróis. A micoflora dos enchidos tem, por isso, manifesta influência na decomposição, afectando assim negativamente a qualidade deste tipo de produtos. Foram efectuados isolamentos e contagens da micoflora de contaminação de morcelas, chouriços, farinheiras e linguiças, embalados em atmosferas protectoras, em duas fases distintas do circuito comercial, de forma a avaliar o teor destes agentes: no inicio e no fim do prazo de validade do produto. Cada tipo de colónia isolada foi observada para caracterização das morfologias (macro e microscópica) e para identificação bioquímica até à espécie. A identificação das leveduras foi realizada em galerias bioquímicas convencionais e a leitura das provas bioquímicas foi efectuada automaticamente através do sistema ATB. Cada levedura identificada foi submetida a testes para detecção de actividade lipolítica. Utilizando Tributirina Agar foram consideradas positivas as colónias que exibiam halos transparentes na sua periferia. Na primeira fase do estudo (início do prazo de validade)...

Mycobiota and occurrence of Fumonisin B1 in wheat harvested in Southern Brazil

Mendes,Gabriela da Rocha Lemos; Reis,Tatiana Alves dos; Corrêa,Benedito; Badiale-Furlong,Eliana
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2015 EN
Relevância na Pesquisa
27.53%
Although fumonisin B1 (FB1) is a mycotoxin poorly studied in wheat, the Fusarium genus fungi have been frequently detected in this cereal, especially in subtropical regions such as southern Brazil, thus studies on their occurrence are required. This study evaluated the mycobiota, water activity and the presence of FB1 in 11 wheat cultivars grown in the northeastern and northwestern of Rio Grande do Sul. The mycobiota was identified by morphological and molecular techniques and FB1 was determined by liquid chromatography with fluorescence detection (HPLC-FL). Microbial counts lower than 103CFU were found in the samples, and the fungi genera Phoma, Cladosporium and Fusarium were the most frequent. Despite this, 54% of the samples were contaminated with FB1 at levels ranging from 958 to 4,906µg FB1 kg-1.

Integument Mycobiota of Wild European Hedgehogs (Erinaceus europaeus) from Catalonia, Spain

Molina-López, R. A.; Adelantado, C.; Arosemena, E. L.; Obón, E.; Darwich, L.; Calvo, M. A.
Fonte: International Scholarly Research Network Publicador: International Scholarly Research Network
Tipo: Artigo de Revista Científica
Publicado em 25/09/2012 EN
Relevância na Pesquisa
27.53%
There are some reports about the risk of manipulating wild hedgehogs since they can be reservoirs of potential zoonotic agents like dermatophytes. The aim of this study was to describe the integument mycobiota, with special attention to dermatophytes of wild European hedgehogs. Samples from spines and fur were cultured separately in Sabouraud dextrose agar (SDA) with antibiotic and dermatophyte test medium (DTM) plates. Nineteen different fungal genera were isolated from 91 cultures of 102 hedgehogs. The most prevalent genera were Cladosporium (79.1%), Penicillium (74.7%), Alternaria (64.8%), and Rhizopus (63.7%). A lower prevalence of Aspergillus (P = 0,035; χ2 = 8,633) and Arthrinium (P = 0,043; χ2 = 8,173) was isolated during the spring time and higher frequencies of Fusarium (P = 0,015; χ2 = 10,533) during the autumn. The prevalence of Acremonium was significantly higher in young animals (70%, 26/37) than in adults (30%, 11/37) (P = 0,019; χ2 = 5,915). Moreover, the majority of the saprophytic species that grew at the SDA culture were also detected at the DTM. Finally, no cases of ringworm were diagnosed and no dermatophytes spp. were isolated. Concluding, this study provides the first description of fungal mycobiota of the integument of wild European hedgehogs in Spain...

Inhibitory Effects of Na-Hypochlorite and Heating on the Mycobiota Associated with Fruits or Juice of Passion (Passiflora edulis Sims) in Uganda

Ismail, Mady A.
Fonte: The Korean Society of Mycology Publicador: The Korean Society of Mycology
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.86%
A total of 34 species belonging to 21 genera of fungi were recorded on passion fruits of both pure and hybrid origin in Uganda, however, the pure type exhibited wider spectrum (28 species and 16 genera) than the hybrid type (21 & 15). Also, yeasts (unidentified and Rhodotorula mucilaginosa) were also encountered in high numbers. Moreover, the mean count of all mycobiota obtained from the pure type was higher than that of hybrid, despite the bigger size of the later. Members of yeasts and Cladosporium followed by Phoma, Penicillium, Fusarium and Alternaria species dominated on passion fruits of pure origin, while only C. cladosporioides, F. solani and yeasts dominated on the hybrid type. Treatment with Na-hypochlorite exhibited inhibitory effects on the total mycobiotic propagules as well as the dominant species from fruits of both types. The current results, therefore, suggest the use of Na-hypochlorite to control the post-harvest mycobiota associated with passion fruits. Regarding the mycobiota contaminating passion juice, yeasts were found to be the major contaminants with Candida parapsilosis being the most common. Moulds constituted only a minor proportion with Acremonium strictum followed by Fusarium chlamydosporum, F. moniliforme...

The Mycobiota of Air Inside and Outside the Meju Fermentation Room and the Origin of Meju Fungi

Kim, Dae-Ho; Kim, Sun-Hwa; Kwon, Soon-wo; Lee, Jong-Kyu; Hong, Seung-Beom
Fonte: The Korean Society of Mycology Publicador: The Korean Society of Mycology
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.53%
The fungi on Meju are known to play an important role as degrader of macromolecule of soybeans. In order to elucidate the origin of fungi on traditional Meju, mycobiota of the air both inside and outside traditional Meju fermentation rooms was examined. From 11 samples of air collected from inside and outside of 7 Meju fermentation rooms, 37 genera and 90 species of fungi were identified. In outside air of the fermentation room, Cladosporium sp. and Cladosporium cladosporioides were the dominant species, followed by Cladosporium tenuissimum, Eurotium sp., Phoma sp., Sistotrema brinkmannii, Alternaria sp., Aspergillus fumigatus, Schizophyllum commune, and Penicillium glabrum. In inside air of the fermentation room, Cladosporium sp., Aspergillus oryzae, Penicillium chrysogenum, Asp. nidulans, Aspergillus sp., Cla. cladosporioides, Eurotium sp., Penicillium sp., Cla. tenuissimum, Asp. niger, Eur. herbariorum, Asp. sydowii, and Eur. repens were collected with high frequency. The concentrations of the genera Aspergillus, Eurotium, and Penicillium were significantly higher in inside air than outside air. From this result and those of previous reports, the origin of fungi present on Meju was inferred. Of the dominant fungal species present on Meju...

What is living on your dog's skin? Characterization of the canine cutaneous mycobiota and fungal dysbiosis in canine allergic dermatitis

Meason-Smith, Courtney; Diesel, Alison; Patterson, Adam P.; Older, Caitlin E.; Mansell, Joanne M.; Suchodolski, Jan S.; Rodrigues Hoffmann, Aline
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.72%
To characterize the skin-associated fungal microbiota (mycobiota) in dogs, and to evaluate the influence of body site, individual dog or health status on the distribution of fungi, next-generation sequencing was performed targeting the internal transcribed spacer region. A total of 10 dogs with no history of skin disease were sampled at 10 distinct body sites consisting of haired and mucosal skin, and 8 dogs with diagnosed skin allergies were sampled at six body sites commonly affected by allergic disease. Analysis of similarities revealed that body site was not an influencing factor on membership or structure of fungal communities in healthy skin; however, the mucosal sites were significantly reduced in fungal richness. The mycobiota from body sites in healthy dogs tended to be similar within a dog, which was visualized in principle coordinates analysis (PCoA) by clustering of all sites from one dog separate from other dogs. The mycobiota of allergic skin was significantly less rich than that of healthy skin, and all sites sampled clustered by health status in PCoA. Interestingly, the most abundant fungi present on canine skin, across all body sites and health statuses, were Alternaria and Cladosporium—two of the most common fungal allergens in human environmental allergies.

Constituição da micobiota aérea de bibliotecas públicas no município de Fortaleza, Estado do Ceará, Brasil; Constitution of the airborne mycobiota of public libraries in the municipality of Fortaleza, Ceará, Brazil

Pantoja, Lydia Dayanne Maia; Universidade Federal do Ceará - UFC; Rizzo, Roberta Silva; Universidade Estadual do Ceará.; Carvalho, Bruno Silva; Universidade Estadual do Ceará.; Ferreira, Victor Conde; Universidade Estadual do Ceará.; Galas, Kandarpa S
Fonte: Departamento de Ciência da Informação – UFSC Publicador: Departamento de Ciência da Informação – UFSC
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Pesquisa Experimental; Formato: application/pdf
Publicado em 05/08/2012 POR
Relevância na Pesquisa
37.53%
http://dx.doi.org/10.5007/1518-2924.2012v17n34p31Estudos da qualidade do ar de diferentes tipos de ambientes fechados vêm merecendo atenção, devido principalmente à grande quantidade de substratos favoráveis à ação de agentes biodegradadores, como os fungos anemófilos, sobre os acervos escritos e digitais. A presente pesquisa identificou e monitorou a micobiota aérea de 04 bibliotecas públicas de referência no município de Fortaleza-CE, durante 24 meses (janeiro/2009 a dezembro/2010). A coleta foi realizada pelo método da sedimentação passiva das partículas presentes no ar em placas de Petri contendo Ágar Batata Dextrose (Himedia®). Essas placas foram dispostas, bimestralmente, em diversos setores das bibliotecas: acervo de obras, área de pesquisa, sala de leitura, sala de documentos históricos e em áreas administrativas (secretaria), perfazendo 240 amostras. As placas foram incubadas por 7 dias (26 – 28 °C) com posterior contagem global e identificação das colônias fúngicas baseada nas análises macro e micromorfológicas. Nas bibliotecas A, B, C e D foram contabilizadas 3.347, 5.874, 6.328 e 5.333 colônias fúngicas, respectivamente e identificados 61 diferentes achados fúngicos (34 gêneros e 27 espécies fúngicas)...

Contribution to the study of the mycobiota present in the natural habitats of Histoplasma capsulatum: an integrative study in Guerrero, Mexico

Ulloa,Miguel; Lappe,Patricia; Aguilar,Samuel; Park,Houng; Pérez-Mejía,Amelia; Toriello,Conchita; Taylor,Maria Lucia
Fonte: Instituto de Biología Publicador: Instituto de Biología
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2006 EN
Relevância na Pesquisa
37.53%
The mycobiota present in natural habitats of Histoplasma capsulatum was determined in samples of bat guano, poultry droppings, and intestinal contents of bats. The following fungi were isolated: 1) from bat guano: the ascomycetes Aphanoascus fulvescens, Gymnascella citrina, Gymnoascus dankaliensis, and Chaetomidium fimeti; the mitosporic fungi Aspergillus flavo-furcatis, A. terreus, A. terreus var. aureus, Penicillium spp., Malbranchea aurantiaca, and Sporothrix sp.; and the yeasts Candida catenulata, C. ciferrii, C. famata var. flareri, C. guilliermondii var. guilliermondii, and Rhodotorula spp. 2) from poultry droppings: the coelomycete Phoma sp.; and the yeasts C. albicans, C. catenulata, C. ciferrii, C. famata var. flareri, C. tropicalis, Cryptococcus albidus, Trichosporon moniliiforme, and Trichosporon spp. 3) from the intestinal contents of insectivorous, hematophagous, nectarivorous, and frugivorous bats: Ch. fimeti; the mitosporic fungi Aspergillus candidus, A. flavo-furcatis, A. sulphureus, A. sydowii, A. terreus, A. versicolor, Aspergillus sp., M. aurantiaca, Gliomastix murorum, and Scopulariopsis sp.; and C. famata var. flareri, C. lipolytica, Cr. albidus, and Trichosporon spp. Most of the species found are first records for these substrata and environments in Mexico. The coexistence with H. capsulatum was demonstrated by high specific antibody titers in ELISA serological method...