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Identificação de marcadores moleculares ligados a gene de resistência ao vírus do mosaico (PRSV-W) em melão (Cucumis melo L.).; Identification of molecular markers linked to a resistance gene to prsv-w in melon (Cucumis melo L).

Teixeira, Ana Paula Matoso
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 17/09/2004 PT
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45.95%
A importância da cultura do meloeiro é crescente no Brasil, sobretudo na região Nordeste, tanto pelo volume comercializado como por ser estabelecida geralmente em pequenas propriedades. Diversas enfermidades acometem esta cultura, destacando-se as viroses. Dentre estas, o mosaico, causado pelo Papaya ringspot virus - estirpe melancia (PRSV-W) é das mais importantes. Dentre as estratégias de controle desta doença, o emprego de cultivares resistentes apresenta-se como um método prático e eficiente. O objetivo deste trabalho foi identificar marcadores moleculares do tipo AFLP ligados ao gene Prv1 de resistência a PRSV-W em melão que futuramente pudessem ser utilizados em seleção assistida por marcadores. Para isto, foram analisadas duas linhagens quase-isogênicas (LQI-R e LQI-S) do tipo Amarelo CAC contrastantes para resistência ao vírus e uma linhagem do tipo Charentais doadora do gene de resistência. A LQI resistente foi obtida através de cruzamento entre a linhagem doadora do gene (LRD) e a linhagem recorrente (LQI-S), seguido de cinco retrocruzamentos de plantas resistentes com a linhagem recorrente. A porcentagem do genoma do parental recorrente recuperado na LQI-R foi de aproximadamente 98,44%. Polimorfismos entre as linhagens resistentes e a suscetível foram considerados marcadores candidatos ligados ao gene de reistência Prv1. Para análise de co-segregação entre gene e marcadores candidatos...

Identificação de marcador molecular ligado ao gene Pm-1 que confere resistência a raça 1 de oídio (Podosphaera xanthii) em melão (Cucumis melo L.); Identification of molecular marker linked to the Pm-1 gene that confers resistance to race 1 of to powdery mildew (Podosphaera xanthii) in melon (Cucumis melo L.)

Silva-Barreto, Fatima Aparecida da
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 20/07/2007 PT
Relevância na Pesquisa
55.92%
A cultura do meloeiro é de grande importância econômica no Brasil, sendo a região Nordeste a principal produtora e exportadora do fruto. A baixa resistência aos principais patógenos é um dos fatores limitantes para a competitividade brasileira da cultura no mercado internacional. Uma das doenças mais importantes é o oídio, causado por Podosphaera xanthii. Geralmente, o controle de P. xanthii é obtido com o uso de fungicidas. Porém, é necessário empregar medidas mais econômicas e que não agridam o ambiente, como a utilização de cultivares resistentes. O objetivo deste trabalho foi identificar marcadores moleculares de vários tipos ligados ao gene Pm-1 de resistência à raça 1 de P. xanthii com o intuito de auxiliar programas de melhoramento genético. Para tanto foram utilizadas duas linhagens quase-isogênicas (LQI) de melão AF426pm1 (P1) e AF426Pm1 (P2), ambas pertencentes à variedade inodorus, que são contrastantes para ausência e presença, respectivamente, do gene Pm-1. Para a análise de co-segregação entre gene e marcadores candidatos, foi utilizada uma população de retrocruzamento RC1F1, fenotipada para resistência a raça 1 de oídio, obtida a partir do cruzamento entre linhagens. As técnicas de LM-PCR e AFLP resultaram em marcadores polimórficos...

Desenvolvimento de marcador molecular para resistência a Tobacco mosaic virus e herança da resistência a Meloidogyne incognita raça 3 em tabaco; Development of molecular marker for resistance to Tobacco mosaic virus and heredity of resistance to Meloidogyne incognita race 3 in tobacco

Dalla Valle, Raphaelle Komatsu
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 05/09/2008 PT
Relevância na Pesquisa
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Este projeto objetivou desenvolver um marcador molecular ligado ao gene de resistência a Tobacco mosaic virus (TMV), em vista da necessidade de aprimorar os métodos de melhoramento de plantas para atender crescentes demandas de produtividade. O outro objetivo deste trabalho foi a avaliação de uma população segregante F2 e de retrocruzamento (RC1F1) a Meloidogyne incognita raça 3, oriunda do cruzamento das cultivares comerciais Coker 176 (C176) e Coker 371 Gold (C371G). Para o desenvolvimento do marcador ligado ao gene de resistência a TMV, o gene N, foram desenvolvidos iniciadores específicos para regiões conservadas (TIR, NBS e LRR) deste gene com base em sua seqüência. Estes iniciadores foram utilizados para amplificar um marcador cuja ligação ao referido gene foi confirmada em 200 indivíduos de população segregante F2 oriunda do cruzamento entre uma linhagem resistente (Coker176) e outra suscetível ao vírus (Kentucky326). A proporção entre o número de plantas resistentes e suscetíveis (154:46) não diferiu estatisticamente daquela esperada no caso de segregação de um gene dominante de resistência, que seria de 3:1. Os resultados indicaram que o marcador e o gene estão proximamente ligados segundo taxa de recombinação...

Mating system of a population of Myracrodruon urundeuva F.F. & M.F. Allemão using the fAFLP molecular marker

Freitas, Miguel L.M.; Sebbenn, Alexandre M.; Moraes, Mario L.T.; Lemos, Eliana G.M.
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: 425-431
ENG
Relevância na Pesquisa
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); The mating system and genetic diversity were studied in a natural population of Myracrodruon urundeuva originating from 30 open-pollinated trees at the Paulo de Faria Ecological Station, SP, Brazil. The progenies were planted on the Teaching and Research Farm of the Ilha Solteira Engineering School, UNESP. Using the fAFLP molecular marker, eleven loci were selected to study the mating system. The mating system was analyzed using the multilocus mixed-mating model. The estimates of genetic divergence between pollen and ovule allele frequencies were significant for eight loci, suggesting nonrandom outcrossing. The estimates of the multilocus outcrossing rate revealed that M. urundeuva possesses a mating system with a predominance of outcrossing events (theta = 0.940 ± 0.086). The estimates of coancestry among plants within progenies (theta = 0.185) was higher than that expected for half-sib progenies (0.125) and the indirect estimate of the correlation of outcrossed paternity within progeny arrays (r p) was 0.403, suggesting that progenies have a high proportion of full-sibs. Result analysis suggests the need for the application of biometric models that take into account deviations from random outcrossing in the estimations of genetic parameters for quantitative traits and the need for retaining large sample sizes in order to preserve genetic variability.

SARP2 as molecular marker of human sperm morphology; SARP2 como marcador molecular de morfologia de espermatozóides humanos

Santos, Nadine Castelhano
Fonte: Universidade de Aveiro Publicador: Universidade de Aveiro
Tipo: Dissertação de Mestrado
ENG
Relevância na Pesquisa
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A fosforilação proteica resulta de um equilíbrio entre fosfatases e quinases constituindo o principal regulador da maioria dos mecanismos existentes nos sistemas biológicos. Muitas doenças (cancro, diabetes, doenças neurodegenerativas, infertilidade, etc.) estão associadas à disrupção deste equilíbrio levando a mudanças nas actividades enzimáticas das proteínas fostatase e quinase. A proteína fosfatase 1 (PP1) é a principal fosfatase serina/treonina sendo ubíqua e altamente conservada nos eucariotas. A PP1 controla várias funções, tais como, a divisão celular, a transcrição, a neurotransmissão, a mobilidade dos espermatozóides, entre outras. A fosforilação proteica é uma das formas de os espermatozóides adquirirem funcionalidade, sendo a proteína PP1γ2 a isoforma mais fortemente enriquecida. Assim, no interior do espermatozóide podemos encontrar a PP1γ2 associada ao comprimento total da cauda e à região equatorial da cabeça, sugerindo uma possível função na mobilidade e reacção acrossómica, respectivamente. Existem inúmeras proteínas que interagem com a PP1γ2 que têm vindo a contribuir para a compreensão do seu papel nas funções fisiológicas do espermatozóide. Apesar de existirem outros...

Use of molecular marker techniques in seed testing by Brazilian seed companies

Della Vecchia,P.T.; Silva,C.A.R. da; Terenciano-Sobrinho,P.
Fonte: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" Publicador: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/1998 EN
Relevância na Pesquisa
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Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD, microsatelites, AFLP) for genetic purity determinations in seed testing. As far as we know, no Brazilian seed company is using, on regular basis, RAPD or other molecular marker techniques in the determination of genetic purity in seed testing. Most of these are using morphological or physiological traits expressed by seed, seedling or mature plant and/or electrophoresis of seed or seedling proteins/isoenzymes for that purpose. Main reasons for that are: DNA molecular marker techniques are relatively new; lack of specialized personnel to run DNA molecular marker assays on routine basis; higher cost/sample when compared to proteins/isoenzymes electrophoresis.

Mating system of a population of Myracrodruon urundeuva F.F. & M.F. Allemão using the fAFLP molecular marker

Freitas,Miguel L.M.; Sebbenn,Alexandre M.; Moraes,Mario L.T.; Lemos,Eliana G.M.
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2004 EN
Relevância na Pesquisa
65.88%
The mating system and genetic diversity were studied in a natural population of Myracrodruon urundeuva originating from 30 open-pollinated trees at the Paulo de Faria Ecological Station, SP, Brazil. The progenies were planted on the Teaching and Research Farm of the Ilha Solteira Engineering School, UNESP. Using the fAFLP molecular marker, eleven loci were selected to study the mating system. The mating system was analyzed using the multilocus mixed-mating model. The estimates of genetic divergence between pollen and ovule allele frequencies were significant for eight loci, suggesting nonrandom outcrossing. The estimates of the multilocus outcrossing rate revealed that M. urundeuva possesses a mating system with a predominance of outcrossing events (theta = 0.940 ± 0.086). The estimates of coancestry among plants within progenies (theta = 0.185) was higher than that expected for half-sib progenies (0.125) and the indirect estimate of the correlation of outcrossed paternity within progeny arrays (r p) was 0.403, suggesting that progenies have a high proportion of full-sibs. Result analysis suggests the need for the application of biometric models that take into account deviations from random outcrossing in the estimations of genetic parameters for quantitative traits and the need for retaining large sample sizes in order to preserve genetic variability.

A conservative region of the mercuric reductase gene (merA) as a molecular marker of bacterial mercury resistance

Sotero-Martins,Adriana; Jesus,Michele Silva de; Lacerda,Michele; Moreira,Josino Costa; Filgueiras,Ana Luzia Lauria; Barrocas,Paulo Rubens Guimarães
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2008 EN
Relevância na Pesquisa
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The most common bacterial mercury resistance mechanism is based on the reduction of Hg(II) to Hg0, which is dependent of the mercuric reductase enzyme (MerA) activity. The use of a 431 bp fragment of a conservative region of the mercuric reductase (merA) gene was applied as a molecular marker of this mechanism, allowing the identification of mercury resistant bacterial strains.

Multiple molecular marker testing (p53, C-Ki-ras, c-erbB-2) improves estimation of prognosis in potentially curative resected non-small cell lung cancer

Schneider, P M; Praeuer, H W; Stoeltzing, O; Boehm, J; Manning, J; Metzger, R; Fink, U; Wegerer, S; Hoelscher, A H; Roth, J A
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.01%
A prospective study was performed in patients with non-small cell lung cancer (NSCLC) to evaluate the prognostic importance of multiple molecular marker (p53, c-Ki-ras, c-erbB-2) testing. 103 patients with potentially curative resections (RO resection) for NSCLC in histopathological stages I–IIIA were included. SSCP analysis and DNA sequencing for p53 and c-Ki-ras genes were performed on paired tumour and normal lung tissue samples and immunohistochemistry (c-erbB-2) was done on frozen tissue sections with a specific anti-c-erbB-2 monoclonal antibody. 46/103 (44.6%) NSCLC showed p53 mutations and 17/103 (16.5%) c-Ki-ras mutations including 12/37 (32.4%) adenocarcinomas. Overexpression of c-erbB-2 (p185) was detected in 56/103 (54.4%) tumours. 24/103 (23.3%) NSCLC were negative for alterations in all 3 parameters (c-Ki-ras, p53 and p185) whereas 79/103 (76.7%) were positive for at least one of the 3 parameters. In a regression model including a multiple molecular marker parameter (negative for all 3 markers versus positive for at least one marker), histopathological stage (P< 0.00001), respectively the pT (P< 0.01) and pN (P< 0.00001) categories and the multiple molecular marker parameter (P< 0.01) were of significant prognostic importance. This study demonstrates that testing 3 molecular markers (c-Ki-ras...

Determination of genetic structure of germplasm collections: are traditional hierarchical clustering methods appropriate for molecular marker data?

Odong, T. L.; van Heerwaarden, J.; Jansen, J.; van Hintum, T. J. L.; van Eeuwijk, F. A.
Fonte: Springer-Verlag Publicador: Springer-Verlag
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
45.95%
Despite the availability of newer approaches, traditional hierarchical clustering remains very popular in genetic diversity studies in plants. However, little is known about its suitability for molecular marker data. We studied the performance of traditional hierarchical clustering techniques using real and simulated molecular marker data. Our study also compared the performance of traditional hierarchical clustering with model-based clustering (STRUCTURE). We showed that the cophenetic correlation coefficient is directly related to subgroup differentiation and can thus be used as an indicator of the presence of genetically distinct subgroups in germplasm collections. Whereas UPGMA performed well in preserving distances between accessions, Ward excelled in recovering groups. Our results also showed a close similarity between clusters obtained by Ward and by STRUCTURE. Traditional cluster analysis can provide an easy and effective way of determining structure in germplasm collections using molecular marker data, and, the output can be used for sampling core collections or for association studies.

The GCP molecular marker toolkit, an instrument for use in breeding food security crops

Van Damme, Veerle; Gómez-Paniagua, Humberto; de Vicente, M. Carmen
Fonte: Springer Netherlands Publicador: Springer Netherlands
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
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Crop genetic resources carry variation useful for overcoming the challenges of modern agriculture. Molecular markers can facilitate the selection of agronomically important traits. The pervasiveness of genomics research has led to an overwhelming number of publications and databases, which are, nevertheless, scattered and hence often difficult for plant breeders to access, particularly those in developing countries. This situation separates them from developed countries, which have better endowed programs for developing varieties. To close this growing knowledge gap, we conducted an intensive literature review and consulted with more than 150 crop experts on the use of molecular markers in the breeding program of 19 food security crops. The result was a list of effectively used and highly reproducible sequence tagged site (STS), simple sequence repeat (SSR), single nucleotide polymorphism (SNP), and sequence characterized amplified region (SCAR) markers. However, only 12 food crops had molecular markers suitable for improvement. That is, marker-assisted selection is not yet used for Musa spp., coconut, lentils, millets, pigeonpea, sweet potato, and yam. For the other 12 crops, 214 molecular markers were found to be effectively used in association with 74 different traits. Results were compiled as the GCP Molecular Marker Toolkit...

Evaluation of HOXB13 as a molecular marker of recurrent prostate cancer

JEONG, TAE-O; OH, KYUNG-JIN; NGUYEN, NGUYEN THI XUAN; KIM, YOUNG-RANG; KIM, MIN SOO; LEE, SANG DON; RYU, SOO BANG; JUNG, CHAEYONG
Fonte: D.A. Spandidos Publicador: D.A. Spandidos
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
45.95%
Many patients with prostate cancer have disease recurrence following surgical removal of tumors and fail to respond to androgen ablation therapy. Despite the existence of a number of clinical/pathological factors, it is not possible to predict which patients will fall into this category. The results of our previous studies demonstrated that the HOXB13 homeodomain protein plays a key role in the development of prostate cancer and the progression of this malignancy. In addition, HOXB13 has been reported to predict estrogen-resistant breast cancer tumors. The purpose of this study was to investigate whether HOXB13 could be used as a molecular marker to predict prostate cancer recurrence. To examine the role of HOXB13 as a molecular marker with clinical/pathological data, the expression of HOXB13 was compared using immunohistochemistry in 57 organ-confined prostate cancer tumors obtained by radical prostatectomy. There was no significant correlation between the expression of HOXB13 and most clinical/pathological parameters, including tumor margin, invasion, pathological stage and risk level. The HOXB13 expression levels correlated with the Gleason score and there was a positive correlation with the pre-operative prostate specific antigen (PSA) levels. Accordingly...

Identification of a molecular marker linked to apomixis in Brachiaria humidicola (Poaceae)

ZORZATTO, C.; CHIARI, L.; BITENCOURT, G. de A.; VALLE, C. B. do; LEGUIZAMON, G. O. de C.; SCHUSTER, I.; PAGLIARINI, M. S.
Fonte: Plant Breeding, v. 129, n. 6, p. 734-736, 2010. Publicador: Plant Breeding, v. 129, n. 6, p. 734-736, 2010.
Tipo: Artigo em periódico indexado (ALICE)
PT_BR
Relevância na Pesquisa
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A bulked segregant analysis using RAPD technique was carried out to identify molecular markers linked to apomixis in a Brachiaria humidicola F1 population that segregated 1 : 1 for the mode of reproduction (apomixis and sexual). A marker related to the apo-locus was found. Segregation data, together with this marker were used to generate a map of the region. This marker was located at 4.61 cM of the target locus, and it can be used in deploying marker-assisted selection for mode of reproduction in the hybrid progenies of this species.; 2010

Building a transcriptome molecular marker platform for diagnosis of fungal plant pathogens.

HERAI, R.; DITA, M.; WAALWIJK, C.; FERREIRA, G.; SOUZA, M.; KEMA, G.; FALCAO, P.; GIACHETTO, P.; YAMAGISHI, M.
Fonte: In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 8., 2012, Campinas. Abstract book... Ribeirão Preto: AB3C, 2012. Publicador: In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 8., 2012, Campinas. Abstract book... Ribeirão Preto: AB3C, 2012.
Tipo: Resumo em anais de congresso (ALICE) Formato: Não paginado.
EN
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By using an Expressed Sequence Tag (EST) library from all four races of Foc, we developed a molecular marker platform based on single sequence repeats (SSR).; 2012; X-MEETING 2012.

Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius L.)

Boersma, J.; Pallotta, M.; Li, C.D.; Buirchell, B.; Sivasithamparam, K.; Yang, H.
Fonte: Cellular & Molecular Biology Letters Publicador: Cellular & Molecular Biology Letters
Tipo: Artigo de Revista Científica
Publicado em //2005 EN
Relevância na Pesquisa
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A mapping population of F8 derived recombinant inbred lines (RILs) was established from a cross between a domesticated breeding line 83A:476 and a wild type P27255 in narrow-leaf lupin (Lupinus angustifolius L.). The parents together with the 89 RILs were subjected to DNA fingerprinting using microsatellite-anchored fragment length polymorphism (MFLP) to rapidly generate DNA markers to construct a linkage map. Five hundred and twenty two unique markers of which 21% were co-dominant, were generated and mapped. Phenotypic data for the domestication traits: mollis (soft seeds), leucospermus (white flower and seed colour); Lentus (reduced pod-shattering), iucundis (low alkaloid), Ku (early flowering) and moustache pattern on seed coats; were included. Three to 7 molecular markers were identified within 5cM of each of these domestication genes. The anthracnose resistance gene Lanr1 was also mapped. Linkage groups were constructed using MapManager version QTXb20, resulting in 21linkage groups consisting of 7 or more markers. The total map length was 1543cM, with an average distance of 3.4cM between adjacent markers. This is the first published map for a lupin species. The map can be exploited for marker assisted selection for genetic improvement in lupin breeding programs.; http://www.cmbl.org.pl/vol10_nr2.html; Jeffrey G. Boersma...

Temperature Switch PCR (TSP): a gel-based molecular marker technique for investigating single nucleotide polymorphisms

Thanh, L.; Khoo, K.
Fonte: Humana Press Publicador: Humana Press
Tipo: Parte de Livro
Publicado em //2014 EN
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Temperature Switch PCR (TSP) is a robust single-marker single nucleotide polymorphism (SNP) genotyping technique with broad applications in genetic studies of various organisms. The technique consists of a biphasic PCR with two sets of primers, a locus-specific set and a nested locus-specific set. The PCR products can be easily assessed for polymorphism based on different band sizes using agarose gel electrophoresis.; Le Phuoc Thanh and Kelvin Khoo

Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies

Nascimento,Ildon Rodrigues do; Maluf,Wilson Roberto; Figueira,Antônia Reis; Menezes,Cícero Beserra; Resende,Juliano Tadeu Vilela de; Faria,Marcos Ventura; Nogueira,Douglas Willian
Fonte: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" Publicador: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2009 EN
Relevância na Pesquisa
55.94%
The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5+) from susceptible (Sw-5+/Sw-5+) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes (Sw-5/Sw-5+), which confirmed the co-dominant inheritance mechanism of the marker. Fifty seven plants from the isogenic progenies were characterized based on bands pattern: 18 plants (31.6%) were identified as resistant homozygotes, 8 plants (14.0%) as resistant heterozygotes and 31 plants (54.4%) were characterized as susceptible. The SCAR 'Sw-421' marker is an important tool for selection and pyramid resistance alleles, mainly when other resistance sources to the TSWV are available...

Identificação de genótipos de tomateiro resistentes a tospovirus em progênies segregantes com marcador molecular; Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies

Nascimento, Ildon Rodrigues do; Maluf, Wilson Roberto; Figueira, Antônia Reis; Menezes, Cícero Beserra; Resende, Juliano Tadeu Vilela de; Faria, Marcos Ventura; Nogueira, Douglas Willian
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/06/2009 ENG
Relevância na Pesquisa
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O marcador molecular SCAR 'Sw-421' é localizado a 1,0 cM do alelo Sw-5, proveniente de Lycopersicon peruvianum (L.), que confere resistência ao vira-çabeça em tomateiro. Contudo, o mesmo ainda não havia sido testado em populações avançadas de tomateiro. O objetivo do trabalho foi distinguir plantas resistentes homozigotas (Sw-5/Sw-5), resistentes heterozigotas (Sw-5/Sw-5+) e suscetíveis (Sw-5+/Sw-5+) pelo marcador SCAR 'Sw-421'. As amplificações de bandas de 940 pb e 900 pb caracterizaram os genótipos resistentes homozigotos e suscetíveis, respectivamente. Duas bandas (900 pb e 940 pb) foram observadas nos genótipos heterozigotos, confirmando a herança codominante do marcador. De 57 plantas das progênies isogênicas avaliadas 18 (31,6%) plantas foram caracterizadas como resistentes, 8 (14,0%) como heterozigotas e 31 (54,4%) plantas suscetíveis. O marcador molecular SCAR 'Sw-421' constitui importante ferramenta para seleção e piramidação de alelos de resistência, especialmente quando se utilizam outras fontes de resistência ao vira-cabeça, como por exemplo, a fonte Rey de los Tempranos.; The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.)...

PRIMER NUMBERS OTIMIZATION FOR RAPD TO DETECT GENETIC VARIABILITY AMONG HAIRY BEGGARTICKS ACCESS; OTIMIZAÇÃO DO NÚMERO DE PRIMERS EMPREGADOS EM RAPD PARA DETECTAR VARIABILIDADE GENÉTICA ENTRE ACESSOS DE PICÃO-PRETO

VIDAL, Ribas Antonio; LAMEGO, Fabiane Pinto; NUNES, Anderson Luis
Fonte: UFPR Publicador: UFPR
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Artigo Avaliado pelos Pares Formato: application/pdf
Publicado em 06/03/2006 ENG
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Molecular markers such as RAPD (Random Amplified Polymorphic DNA) are among the most widely used because they are easy to apply, quick and with low cost. With this marker it is possible to evaluate the structure and the genetic diversity in weed and crop populations. The objective of this work was to evaluate the viability of primer number otimization of RAPD molecular marker to know the genetic similarity of target populations. The data bank of two different experiments conducted with hairy beggarticks (Bidens pilosa L.) acesses, at Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, were evaluated on the effects of the primer numbers on the genetic variability. It was possible to otimize the primer number used by RAPD marker. When few primers promoted good band formation in each population, large number of prime will be necessary for the adequate estimative of genetic variability.; Marcadores moleculares do tipo RAPD (polimorfismo de DNA amplificado ao acaso) estão entre os mais utilizados devido a sua facilidade de uso, rapidez e baixo custo. Através deste tipo de marcador é possível avaliar a estrutura e diversidade genética em populações naturais (plantas daninhas) e melhoradas. O objetivo deste trabalho foi avaliar a viabilidade da redução no número de primers utilizados em marcador molecular RAPD para conhecimento da variabilidade genética em populações de plantas daninhas. O banco de dados de dois trabalhos diferentes com acessos de picão-preto (Bidens pilosa L.)...

Genetic molecular analysis of Coffea arabica (Rubiaceae) hybrids using SRAP markers

Kumar Mishra,Manoj; Suresh,Narayana; Bhat,Asha M.; Suryaprakash,Nayani; Satheesh Kumar,Saya; Kumar,Anil; Jayarama,
Fonte: Revista de Biología Tropical Publicador: Revista de Biología Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2011 EN
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In Coffea arabica (arabica coffee), the phenotypic as well as genetic variability has been found low because of the narrow genetic basis and self fertile nature of the species. Because of high similarity in phenotypic appearance among the majority of arabica collections, selection of parental lines for inter-varietals hybridization and identification of resultant hybrids at an early stage of plant growth is difficult. DNA markers are known to be reliable in identifying closely related cultivars and hybrids. Sequence Related Amplified Polymorphism (SRAP) is a new molecular marker technology developed based on PCR. In this paper, sixty arabica-hybrid progenies belonging to six crosses were analyzed using 31 highly polymorphic SRAP markers. The analysis revealed seven types of SRAP marker profiles which are useful in discriminating the parents and hybrids. The number of bands amplified per primer pair ranges from 6.13 to 8.58 with average number of seven bands. Among six hybrid combinations, percentage of bands shared between hybrids and their parents ranged from 66.29% to 85.71% with polymorphic bands varied from 27.64% to 60.0%. Percentage of hybrid specific fragments obtained in various hybrid combinations ranged from 0.71% to 10.86% and ascribed to the consequence of meiotic recombination. Based on the similarity index calculation...