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The microorganisms in the Portuguese national curriculum and primary school text books

Mafra, Paulo; Lima, Nelson
Fonte: World Scientific Publicador: World Scientific
Tipo: Conferência ou Objeto de Conferência
ENG
Relevância na Pesquisa
35.8%
The main aim of the present work is the content analysis of the Portuguese National Curriculum and the Primary School textbooks where microorganisms are concerned. The content analysis through categories created a priori were used as methodology. In all analysed documents the topic microorganisms did not emerge in a clear way. However, several indirect themes related to microorganisms were found in the National Curriculum and textbooks of the Environment Study issue. These themes can be explored with pupils through experimental activities. The Science Education in primary schools can be introduced with proposals of activities involving microorganisms and contributing to a better understanding of the children’s world.

Assessment of antimicrobial effect of Biosilicate (R) against anaerobic, microaerophilic and facultative anaerobic microorganisms

MARTINS, Carlos Henrique Gomes; CARVALHO, Tatiane Cruz; SOUZA, Maria Gorete Mendes; RAVAGNANI, Christian; PEITL, Oscar; ZANOTTO, Edgar Dutra; PANZERI, Heitor; CASEMIRO, Luciana Assirati
Fonte: SPRINGER Publicador: SPRINGER
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
35.83%
This study assessed the antimicrobial activity of a new bioactive glass-ceramic (Biosilicate (R)) against anaerobic, microaerophilic, and facultative anaerobic microorganisms. Evaluation of the antimicrobial activity was carried out by three methods, namely agar diffusion, direct contact, and minimal inhibitory concentration (MIC). For the agar diffusion technique, bio glass-ceramic activity was observed against various microorganisms, with inhibition haloes ranging from 9.0 +/- 1.0 to 22.3 +/- 2.1 mm. For the direct contact technique, Biosilicate (R) displayed activity against all the microorganisms, except for S. aureus. In the first 10 min of contact between the microorganisms and Biosilicate (R), there was a drastic reduction in the number of viable cells. Confirming the latter results, MIC showed that the Biosilicate (R) inhibited the growth of microorganisms, with variations between <= 2.5 and 20 mg/ml. The lowest MIC values (7.5 to <= 2.5 mg/ml) were obtained for oral microorganisms. In conclusion, Biosilicate (R) exhibits a wide spectrum of antimicrobial properties, including anaerobic bacteria.; Brazilian Agencies National Council of Scientific and Technological Development (CNPq, Fundo Verde-Amarelo)[0400604/2004-3]; State of Sao Paulo Research Foundation (FAPESP)...

Bioremediation of Herbicide Velpar K (R) In Vitro in Aqueous Solution with Application of EM-4 (Effective Microorganisms)

Ramos, Marcio Antonio Gomes; Yoshioka, Sergio Akinobu
Fonte: INST TECNOLOGIA PARANA; CURITIBA-PARANA Publicador: INST TECNOLOGIA PARANA; CURITIBA-PARANA
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
35.77%
This work assessed the bioremediation of herbicide Velpar K (R), in vitro in aqueous solution, used against weeds in sugar cane in Sao Paulo state. The herbicide contained Hexazinone and Diuron. It was used the microbial inoculant denominated Effective Microorganisms (EM-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. Results for the depth of cultivation on agar-agar inoculated with EM-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the Velpar K (R) in the gel. The analysis of high performance liquid chromatography ( HPLC) showed that the EM-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron and 70% for hexazinone after 21 days in solution of 2:1 of Velpar K (R)/EM-4 ratio. These results could be useful for planning the bioremediation of contaminated areas with Velpar K (R).

Bioremediation of herbicide velpar K® in vitro in aqueous solution with application of EM-4 (effective microorganisms)

Ramos, Márcio Antônio Gomes; Yoshioka, Sergio Akinobu
Fonte: Brazilian Archives of Biology and Technology Publicador: Brazilian Archives of Biology and Technology
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
35.77%
This work assessed the bioremediation of herbicide Velpar K®, in vitro in aqueous solution, used against weeds in sugar cane in São Paulo state. The herbicide contained Hexazinone and Diuron. It was used the microbial inoculant denominated Effective Microorganisms (EM-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. Results for the depth of cultivation on agar-agar inoculated with EM-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the Velpar K®in the gel. The analysis of high performance liquid chromatography (HPLC) showed that the EM-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron and 70% for hexazinone after 21 days in solution of 2:1 of Velpar K®/EM-4 ratio. These results could be useful for planning the bioremediation of contaminated areas with Velpar K®.

Detecção de microrganismos periodontopatogênicos gram-negativos e quantificação de endotoxina  em bráquetes metálicos, com ou sem utilização de agente antimicrobiano - Estudo in vivo; Detection of Gram-negative periodontopathogenic microorganisms and quantification of endotoxin in orthodontic metallic brackets, with or without use of an antimicrobial agent - An in vivo study

Valdez, Remberto Marcelo Argandoña
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 22/07/2009 PT
Relevância na Pesquisa
35.8%
Empregando a técnica de biologia molecular Checkerboard DNA-DNA Hybridization e o teste Limulus Amebocyte Lysate, os objetivos do presente estudo clínico randomizado in vivo foram avaliar, em bráquetes ortodônticos metálicos: 1) A presença de 16 espécies de microrganismos periodontopatogênicos Gram-negativos pertencentes aos complexos laranja e vermelho, por meio de sondas de DNA; 2) A quantidade de endotoxina bacteriana presente; e 3) A eficácia da utilização do gluconato de clorexidina a 0,12%, sob a forma de bochechos, na redução da contaminação pelas 16 espécies de microrganismos periodontopatogênicos Gram-negativos e na redução da quantidade de endotoxina bacteriana. Participaram do estudo 33 pacientes de 11 a 33 anos de idade, em tratamento com aparelho ortodôntico fixo, nos quais foram colocados randomicamente 3 bráquetes metálicos novos nos pré-molares. Os pacientes do Grupo Controle (n=17) fizeram 2 bochechos semanais com solução placebo, durante 30 dias. Os pacientes do Grupo Experimental (n=16) fizeram bochechos com solução à base de gluconato de clorexidina a 0,12% (Periogard®), da mesma forma que o grupo Controle. Decorridos 30 dias, os 3 bráquetes foram removidos de cada paciente e processados para a detecção dos microrganismos...

Isolamento e seleção de microrganismos para remediação de efluente oleoso da indústria metal-mecânica; Isolation and selection of microorganisms for the oily effluent remediation of the metalworking industry

Queissada, Daniel Delgado
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 15/09/2009 PT
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A indústria metal-mecânica é responsável pela síntese de um dos efluentes oleosos mais complexos e difíceis de tratamento e reuso. Esses efluentes são constituídos, entre outros poluentes, por fluidos de corte, que servem para refrigerar e lubrificar peças metálicas no sistema de usinagem. Quando esses efluentes são tratados biologicamente é importante que se faça isso com microrganismos autóctones do mesmo, pois o uso desses microrganismos (bioaumentação) tende a ser mais eficaz, uma vez que os mesmos já estão adaptados aos poluentes nele existentes. Com este propósito, o presente trabalho teve como objetivo principal o isolamento e seleção de microrganismos para remediação de um efluente metalmecânico através de tratamentos unitários em bioreator do tipo air-lift e por sistema oxidativo UV/H2O2, além do tratamento integrado com ambos os processos. No tratamento biológico foram utilizados os microrganismos autóctones do efluente estudado, além do uso do fungo Aspergillus niger como microrganismo de referência. A caracterização do efluente bruto mostrou que, em decorrência dos parâmetros determinados como, 1495 mg Pt/L de cor, DQO de 9147 mg/L, 53 mg/L de fenóis-totais, 15666 mg/L de ST, 2,5 mgO2/L de OD...

Identificação de microrganismos do trato digestivo de pragas de cana-de-açúcar com atividade enzimática para degradação de substratos lignocelulósicos e potencial para bioconversão de D-xilose em xilitol; Identification of gut microorganisms in sugarcane pests, with enzymatic activity, for degradation of lignocelullosic substrates and bioconversion of D-xylose to xylitol potential

Milano, Heloíze de Souza
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 29/08/2012 PT
Relevância na Pesquisa
35.77%
A necessidade de economias sustentáveis tem aumentado o interesse no desenvolvimento de plataformas microbianas para novos processos, tanto para a produção de biocombustíveis quanto para a síntese de compostos que demandam alta capacidade energética e processamento químico em sua produção. O isolamento de microrganismos, capazes de degradação materiais lignocelulósicos, resistentes a diferentes inibidores e com rendimento elevado na biossíntese de moléculas específicas faz-se necessário para atender tais propósitos. Neste trabalho, microrganismos cultiváveis isolados do trato digestivo de larvas dos insetos pragas de cana-de-açúcar, besouro da raiz, Migdolus fryanus, bicudo da cana, Sphenophorus levis, broca-gigante da cana, Telchin licus licus e de broca-da-cana, Diatraea saccharalis, foram caracterizados quanto a atividade enzimática em fontes de carbono e identificados por técnicas moleculares. Larvas no terceiro ínstar de M. fryanus e S. levis e quinto ínstar de T. licus licus e D. saccharalis foram coletadas em plantios de cana-de-açúcar no interior de São Paulo. Um total de 341 microrganismos cultiváveis foram avaliados quanto a capacidade de degradação de substratos lignocelulósicos, como única fonte de carbono em meio sólido usando o índice de atividade enzimática (I.E.). Os isolados foram identificados por sequênciamento das regiões do 16S rRNA para bactérias...

Acesso a produtos naturais mediante a estratégia de cultivos mistos de endofíticos: o fungo Colletotrichum boninense FLe 8.1 e a actinobactéria Streptomyces albospinus RLe 7; Access to natural products by using the co-culture strategy of endophytic microorganisms: fungus Colletotrichum boninense FLe 8.1 and actinobacteria Streptomyces albospinus RLe 7.

Rodriguez, Andrés Mauricio Caraballo
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 22/02/2013 PT
Relevância na Pesquisa
35.83%
Na literatura encontram-se referências de estudos envolvendo micro-organismos endofíticos, e mais recentemente estudos que avaliam a interação entre micro-organismos o que resulta na modificação, no tipo ou quantidade dos compostos que são produzidos. Neste trabalho foram realizados cultivos simples e mistos do fungo Colletotrichum boninense FLe 8.1 e da actinobacteria Streptomyces albospinus RLe 7, endófiticos isolados de Lychnophora ericoides que pertencem à coleção do Laboratório de Química de Microorganismos (LQMo) da FCFRP-USP, com o objetivo de aumentar suas capacidades de produção de novos compostos com atividade biológica. O cultivo misto, ou co-cultivo, é uma estratégia que tem sido usada para o acesso aos produtos naturais de origem microbiana. Existem poucos relatos de compostos com atividade biológica isolados a partir de S. albospinus e não há relatos de metabólitos secundários obtidos a partir de C. boninense. Nenhum desses micro-organismos tem sido descrito como endofítico na literatura e não existem relatos sobre co-cultivos envolvendo qualquer um deles na busca de compostos bioativos. Juntando as informações geradas através das diferentes técnicas de detecção utilizadas, como TLC, HPLC-DAD...

In vitro antimicrobial activity of AH Plus, EndoREZ and Epiphany against microorganisms

Maekawa, Lilian; Nassri, Maria Renata; Ishikawa, Camila; Martins, Carolina; Chung, Adriana; Koga-Ito, Cristiane
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 469-472
ENG
Relevância na Pesquisa
35.8%
Objective : The aim of the present study was to evaluate the antimicrobial activity of endodontic sealers against microorganisms. Materials and Methods : The agar diffusion method was used. A double base layer of Mueller Hinton agar was done. The microorganisms used were: Candida albicans, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. The wells were obtained by removing a standardized portion of the agar. After the distribution of the sealers, Petri plates were incubated for 24 h. Inhibition halos formed around the wells were measured. Results : Epiphany did not show any antimicrobial activity on the tested microorganisms (without inhibition halo). The AH Plus showed the greatest inhibition halo on C. albicans followed by EndoREZ on S. aureus. EndoREZ also showed greater inhibition halo in comparison to AH Plus on E. faecalis and E. coli. Conclusion : It could be concluded that AH Plus and EndoREZ showed antimicrobial activity against all the tested microorganisms. No antimicrobial activity was observed for Epiphany.

The microorganisms in the Portuguese National Curriculum and Primary School textbooks

Mafra, Paulo; Lima, Nelson
Fonte: World Scientific Publicador: World Scientific
Tipo: Conferência ou Objeto de Conferência
Publicado em //2009 ENG
Relevância na Pesquisa
35.8%
The main aim of the present work is the content analysis of the Portuguese National Curriculum and the Primary School textbooks where microorganisms are concerned. The content analysis through categories created a priori were used as methodology. In all analysed documents the topic microorganisms did not emerge in a clear way. However, several indirect themes related to microorganisms were found in the National Curriculum and textbooks of the Environment Study issue. These themes can be explored with pupils through experimental activities. The Science Education in primary schools can be introduced with proposals of activities involving microorganisms and contributing to a better understanding of the children’s world.; Fundação para a Ciência e a Tecnologia (FCT) - CIFPEC (unidade de investigação 644)

Bioremediation of herbicide velpar K® in vitro in aqueous solution with application of EM-4 (effective microorganisms)

Ramos,Márcio Antônio Gomes; Yoshioka,Sergio Akinobu
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2012 EN
Relevância na Pesquisa
35.77%
This work assessed the bioremediation of herbicide Velpar K®, in vitro in aqueous solution, used against weeds in sugar cane in São Paulo state. The herbicide contained Hexazinone and Diuron. It was used the microbial inoculant denominated Effective Microorganisms (EM-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. Results for the depth of cultivation on agar-agar inoculated with EM-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the Velpar K®in the gel. The analysis of high performance liquid chromatography (HPLC) showed that the EM-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron and 70% for hexazinone after 21 days in solution of 2:1 of Velpar K®/EM-4 ratio. These results could be useful for planning the bioremediation of contaminated areas with Velpar K®.

Integrated evaluation of soil quality after the incorporation of organic matter and microorganisms

Valarini,Pedro J.; Alvarez,Mª Cruz Díaz; Gascó,José Mª; Guerrero,Francisca; Tokeshi,Hasime
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2002 EN
Relevância na Pesquisa
35.8%
The soil quality was evaluated following the addition of organic matter and microorganisms to a clay loam soil collected in Aranjuez (Madrid) under controlled conditions of temperature and moisture, and over a period of three months. The following treatments were carried out: soil (control); soil + 50 t/ha of animal manure (E50); soil + 50 t/ha of animal manure + 30l/ha of effective microorganisms (E50EM); soil + 30 t/ha of combination of various green crop residues and weeds (RC30) and soil + 30 t/ha of combination of various green crop residues and weeds + 30l/ha of effective microorganisms (RC30EM). The soil samples were taken before and after the incubation and analysed using physical, chemical and microbiological parameters. A significant increase in the production of polysaccharides and alkaline phosphatase and esterase enzymes in the treatments E50EM and RC30EM was observed, being in direct correlation with the humification of the organic matter, with the water retention at field capacity, and with the cationic exchange capacity (CEC). It can be concluded that the incorporation of microorganisms EM potentialized the soil biological activity and improved physico-chemical soil properties, contributing to a quick humification of fresh organic matter. Those findings were proved by microbiological activities of exopolysaccharides by alcaline phosphatase and esterase enzymes...

Heavy metal resistance of microorganisms isolated from coal mining environments of Santa Catarina

Castro-Silva,Marcus Adonai; Lima,André Oliveira de Souza; Gerchenski,Ana Valéria; Jaques,Daniela Batista; Rodrigues,André Luis; Souza,Pricila Lima de; Rörig,Leonardo Rubi
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2003 EN
Relevância na Pesquisa
35.77%
The coal mining activity is characterized by the generation of large amount of by-products. One of them is pyrite, which tends to acidify the water, solubilizing heavy metals. As a consequence the environment becomes acid and rich in heavy metals, selecting microorganisms able to survive in this condition, which are of great interest as bioremediation agents. This work describes the isolation and characterization of microorganisms from a coal mining area in Santa Catarina. These microorganisms comprised bacteria, fungi and yeasts resistant to zinc, nickel and cadmium.

Biodiversity of the oleaginous microorganisms in Tibetan Plateau

Li,Shi Lin; Lin,Qiang; Li,Xin Ran; Xu,Hui; Yang,Yun Xi; Qiao,Dai Rong; Cao,Yi
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2012 EN
Relevância na Pesquisa
35.8%
Microbial lipids, which are also known as single cell oils (SCO), are produced by oleaginous microorganisms including oleaginous bacteria, yeast, fungus and algae through converting carbohydrates into lipids under certain conditions. Due to its unique environment having extremely low temperature and anoxia, the Tibetan Plateau is amongst the regions with numerous rare ecotypes such as arid desert, salt marsh, alpine permafrost, hot spring, and lawn. By using a rapid, convenient screening method, we identified 31 strains of oleaginous microorganisms from different habitats in the Tibetan Plateau, which include wetlands, lawn, hot spring, alpine permafrost, and saline-alkali soil. Molecular identity analysis showed that they belong to 15 different species, 7 of which are reported for the first time as lipid-producing microorganisms, that is, Cladosporium sp., Gibberella fujikuro, Ochrobactrum sp., Plectosphaerella sp., Tilletiopsis albescens, Backusella ctenidia, and Davidiella tassiana. The distribution of the oleaginous microorganisms varies with habitats. 11 strains were found in hot spring (35.5%), 10 in farmland (32.3%), 6 in lawn (19.4%), 2 in sand (6.4%), 1 in wetland (3.2%), and 1 in permafrost (3.2%). Carbon utilization analysis indicated that most of these filamentous fungi can use xylose and carboxymethyl cellulose (CMC) as carbon source...

Production of gaba (γ - aminobutyric acid) by microorganisms: a review

Dhakal,Radhika; Bajpai,Vivek K.; Baek,Kwang-Hyun
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2012 EN
Relevância na Pesquisa
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GABA (γ-aminobutyric acid) is a four carbon non-protein amino acid that is widely distributed in plants, animals and microorganisms. As a metabolic product of plants and microorganisms produced by the decarboxylation of glutamic acid, GABA functions as an inhibitory neurotransmitter in the brain that directly affects the personality and the stress management. A wide range of traditional foods produced by microbial fermentation contain GABA, in which GABA is safe and eco-friendly, and also has the possibility of providing new health-benefited products enriched with GABA. Synthesis of GABA is catalyzed by glutamate decarboxylase, therefore, the optimal fermentation condition is mainly based on the biochemical properties of the enzyme. Major GABA producing microorganisms are lactic acid bacteria (LAB), which make food spoilage pathogens unable to grow and act as probiotics in the gastrointestinal tract. The major factors affecting the production of GABA by microbial fermentation are temperature, pH, fermentation time and different media additives, therefore, these factors are summarized to provide the most up-dated information for effective GABA synthesis. There has been a huge accumulation of knowledge on GABA application for human health accompanying with a demand on natural GABA supply. Only the GABA production by microorganisms can fulfill the demand with GABA-enriched health beneficial foods.

Antagonistic properties of some microorganisms isolated from Brazilian tropical savannah plants against Staphylococcus coagulase-positive strain

Ratti,R. P.; Serrano,N. F. G.; Hokka,C. O.; Sousa,C. P.
Fonte: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP Publicador: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2008 EN
Relevância na Pesquisa
35.82%
Endophytic microorganisms are relatively unstudied as potential sources of novel natural products for medical and commercial exploitation. The aim of this work was to investigate some Brazilian tropical savannah trees Cassia leptophylla and Prunus spp. in order to isolate the endophytic microorganisms associated with these plants. The samples were disinfected to eliminate the epiphytic population. Colonies were diluted and displayed as drops in media and growing colonies were inactivated. Staphylococcus coagulase-positive strain was used as indicator microorganism and subjected to the antibioses test. Data showed that the microorganisms isolated from Cassia leptophylla had no inhibition against Staphylococcus. On the other hand, microorganisms isolated from Prunus spp. leaves showed antibacterial activity and inhibited Staphylococcus when cultivated in peptone agar as well as in yeast extract agar. Investigation proceeds in order to classify the isolated microorganisms presenting bioactive substance and exploit the potential of the compounds produced to inhibit the indicator bacteria. Other bioactive properties will be investigated.

Seleção de microrganismos endofíticos com potencialidades para a biorremediação de ambientes contaminados com hidrocarbonetos de petróleo e/ou derivados; Selection of endophytic microorganisms for biorremediation in impacted soils with petroleum hydrocarbons and derivates

OLIVEIRA, Natalia Carvalhaes de
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Medicina Tropical; Medicina Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Medicina Tropical; Medicina
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
35.8%
Endophytic microorganisms live inside plants showing no apparently damage for the host, often assisting in survival of plants, helping its growth with production of phytohormones, phosphates solubilization, nitrogen fixation and enzymes production, or they can metabolize organic contaminants, like petroleum and derivates. This work aimed to isolated and identified endophytic microorganisms of plants present in impacted areas, as well as test their ability in petroleum and its derivatives degradation, identify bacteriocin production, to test their nitrogen fixation capability, phosphate solubilization, indol-acetic acid (IAA) and enzymes production. Plant samples were collected, in an area impacted with asphaltic and mud, were superficially disinfected using 70% ethanol, sodium hypochlorite and sterile distilled water. After macerated and fragmented, the samples were incubated at 30°C for about 72 hours, when growth of microorganisms was observed in culture media: Nutrient Agar, TSA (Tryptone Soya Agar) and King medium. The verification of petroleum and derivatives degradation capacity was performed in ELISA plates, exposing the bacteria to a solution of Minimal Medium, the dye DCPIP solution (2,6-dicloroindofenol sodium salt) and petroleum or derivative tested (burning oil...

Degradação de resíduos sólidos agrícolas por microrganismos isolados de bagaço de cana e seu percolado, e de efluentes de agroindústria.; Degradation of agricultural solid Wastes by microorganisms isolated from sugarcane bagasse, and its percolated, and of effluents from agri-industry.

Silva, Kelly Fernanda Seára da
Fonte: Universidade Federal de Alagoas; BR; Química; Biotecnologia; Programa de Pós-Graduação em Química e Biotecnologia; UFAL Publicador: Universidade Federal de Alagoas; BR; Química; Biotecnologia; Programa de Pós-Graduação em Química e Biotecnologia; UFAL
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
35.83%
Brazil occupies a place of prominence in the productive sector, as well as in the exploitation of agricultural waste, from sugar cane. This use is a fairly widespread practice, both for effluents, mainly vinasse, but also for solid residues, such as filter-cake and bagasse from sugar cane. Thus, at the start of the 2005/06 harvest, samples were collected from residual waters of the S.T.E. (Station for the treatment of effluents), bagasse and from the filtrate liquid (percolate) from bagasse that was accumulated since the 2004/05 harvest, from the industrial processing of sugar cane in S.A. Usina Coruripe Açúcar e Álcool . The target was the isolation of microorganisms that produce extracellular enzymes able to degrade cellulose, hemicellulose, and lignin phenols. There were originally 42 microorganisms isolated, of which 31 were screened for the verification of the production of these and other enzymes, as well as for their morphobiochemistry identification. From these, 29 were identified, with the predominance of the genera Flavobacterium, Chromobaterium and Achromobacter. The other bacterial isolates belong to the genus Corynebacterium, Aeromonas., Bacillus, Clostridium, Citrobacter, Nocardia, Kurthia, Mycobacterium, Serratia...

Degradação de resíduos sólidos agrícolas por microrganismos isolados de bagaço de cana e seu percolado, e de efluentes de agroindústria.; Degradation of agricultural solid Wastes by microorganisms isolated from sugarcane bagasse, and its percolated, and of effluents from agri-industry.

Silva, Kelly Fernanda Seára da
Fonte: Universidade Federal de Alagoas; BR; Química; Biotecnologia; Programa de Pós-Graduação em Química e Biotecnologia; UFAL Publicador: Universidade Federal de Alagoas; BR; Química; Biotecnologia; Programa de Pós-Graduação em Química e Biotecnologia; UFAL
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
35.83%
Brazil occupies a place of prominence in the productive sector, as well as in the exploitation of agricultural waste, from sugar cane. This use is a fairly widespread practice, both for effluents, mainly vinasse, but also for solid residues, such as filter-cake and bagasse from sugar cane. Thus, at the start of the 2005/06 harvest, samples were collected from residual waters of the S.T.E. (Station for the treatment of effluents), bagasse and from the filtrate liquid (percolate) from bagasse that was accumulated since the 2004/05 harvest, from the industrial processing of sugar cane in S.A. Usina Coruripe Açúcar e Álcool . The target was the isolation of microorganisms that produce extracellular enzymes able to degrade cellulose, hemicellulose, and lignin phenols. There were originally 42 microorganisms isolated, of which 31 were screened for the verification of the production of these and other enzymes, as well as for their morphobiochemistry identification. From these, 29 were identified, with the predominance of the genera Flavobacterium, Chromobaterium and Achromobacter. The other bacterial isolates belong to the genus Corynebacterium, Aeromonas., Bacillus, Clostridium, Citrobacter, Nocardia, Kurthia, Mycobacterium, Serratia...

Bioprospection of marine microorganisms: potential and challenges for Argentina

Dionisi,Hebe M; Lozada,Mariana; Olivera,Nelda L
Fonte: Revista argentina de microbiología Publicador: Revista argentina de microbiología
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2012 EN
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The marine environments of Argentina have a remarkable extension, as well as high biological productivity and biodiversity of both macro- and microorganisms. Despite having a great potential for biotechnological applications, the microorganisms inhabiting these ecosystems remain mostly unexplored and unexploited. In this review, we study the research topics and the interactions among Argentinean laboratories, by analyzing current articles published on biotechnology-related marine microbiology by researchers of this country. In addition, we identify the challenges and opportunities for Argentina to take advantage of the genetic potential of its marine microorganisms. Finally, we suggest possible actions that could improve the development of this research field, as well as the utilization of this knowledge to solve societal needs.