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Tolerância cruzada no modelo de inflamação pulmonar alérgica experimental.; Cross-tolerance in a model of experimental allergic lung inflammation.

Balbino, Bianca
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 02/12/2014 PT
Relevância na Pesquisa
27.26%
A tolerância pode ser considerada um dos pilares da imunologia. Sabe-se que a tolerância a um antígeno pode gerar tolerância a outro antígeno não relacionado, fenômeno conhecido como tolerância cruzada. Neste trabalho caracterizamos a tolerância cruzada utilizando a OVA como tolerógeno e extrato de Blomia tropicalis (Bt) ou Hemocianina de Keyhole limpet (KLH) como alérgenos. Verificamos que é possível reproduzir o fenômeno da tolerância cruzada neste modelo de inflamação alérgica induzida tanto pelo KLH quanto pela Bt, com diminuição do infiltrado inflamatório no pulmão, eosinófilos, IgE total e produção de muco. Ainda, a estratégia de utilizar a tolerância cruzada terapeuticamente, i.é., após a sensibilização com KLH, a indução de tolerância cruzada não foi capaz de prevenir a resposta alérgica. Em conjunto, nossos dados mostram que a tolerância à OVA modifica as respostas alérgicas tanto à Bt quanto ao KLH no modelo de inflamação pulmonar experimental de forma profilática, mas que a tolerância cruzada não é eficiente em animais já sensibilizados.; Tolerance is among the Immunology pillars. Experimental data indicate that tolerance towards an antigen can promote tolerance to an unrelated antigen...

A continental scale evaluation of the role of limpet grazing on rocky shores

Coleman, R. A.; Underwood, A. J.; Benedetti-Cecchi, L.; Aberg, P.; Arenas, F.; Arrontes, J.; Castro, J. J.; Hartnoll, R. G.; Jenkins, S. R.; Paula, J.; Santina, P. D.; Hawkins, S. J.
Fonte: Universidade de Évora Publicador: Universidade de Évora
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
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It is critical for our knowledge of biodiversity and ecosystem processes to understand how individual species contribute to ecosystem processes and how these contributions vary in space and time. We used a manipulative field experiment in five locations over 17 º of latitude [from southern Portugal to the Isle of Man (British Isles)] to determine the relative response of rocky intertidal algal assemblages released from control by the grazing of limpets. Response ratios showed that when limpets were removed there was a trend of effects from north to south. In the north, grazing had a strong effect on algal assemblages, but removing grazers reduced spatial variability in assemblages. In the south, the effect of limpet grazing was far weaker and removal of grazers had a much reduced impact on spatial variability. Here we show a clear trophic control of an ecosystem in that grazing by limpets not only determines macroalgal abundance overall but also modifies ecosystem stability via variability in cover of algae.

A case of simultaneous hermaphroditism in the Azorean endemic limpet Patella candei gomesii (Mollusca: Patellogastropoda), a gonochoristic species

Cunha, Luís; Martins, Gustavo M.; Amaral, André; Rodrigues, Armindo
Fonte: Balaban, Philadelphia/Rehovot Publicador: Balaban, Philadelphia/Rehovot
Tipo: Artigo de Revista Científica
Publicado em /11/2007 ENG
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Copyright © 2007, Taylor & Francis.; A case of simultaneous hermaphroditism was observed in Patella candei gomesii, an Azorean endemic species, which has been considered a gonochoristic species. Here we show that simultaneous hermaphroditism can occur within the family Patellidae.

Limpet shell modifications at intertidal hydrothermal vents.

Couto, Ruben P.; Neto, Ana I.; Rodrigues, Armindo
Fonte: Universidade dos Açores Publicador: Universidade dos Açores
Tipo: Conferência ou Objeto de Conferência
Publicado em /08/2010 ENG
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26.84%
45th European Marine Biology Symposium. Edinburgh, Escócia, 23-27 de Agosto de 2010.

Illegal harvesting affects the success of fishing closure areas.

Martins, Gustavo M.; Jenkins, Stuart R.; Hawkins, Stephen J.; Neto, Ana I.; Medeiros, André; Thompson, Richard C.
Fonte: Marine Biological Association of the United Kingdom Publicador: Marine Biological Association of the United Kingdom
Tipo: Artigo de Revista Científica
Publicado em /06/2011 ENG
Relevância na Pesquisa
27.37%
Copyright © Marine Biological Association of the United Kingdom 2010.; There is clear evidence that marine reserves can be used as effective tools to foster the recovery of disturbed ecosystems. In the Azores, intense exploitation of the patellid limpets Patella candei and P. aspera has led to a rapid decline in their populations and subsequent collapse of the fishery in 1985. In 1993, legislation was passed to protect limpets, including the establishment of limpet protected zones (LPZs) where harvesting was completely prohibited. Outside LPZs, a seasonal fishing closure prohibited the harvesting of limpets from October to May. Here we examine the effect of such measures 16 years after they were put into practice. In each of the 3 years examined, limpet density, biomass and size were generally similar both inside and outside the LPZs. In addition, there were clear signs of exploitation as most individual limpets inside the LPZ were smaller than the legal catch size suggesting that illegal harvesting was taking place. Observations confirmed that illegal harvesting of limpets was common both inside and outside LPZs. Lack of enforcement of regulations is therefore a likely reason for the failure of legislation to protect limpet populations and facilitate stock recovery.

Conectividade em populações de lapas nas ilhas da Macaronésia (Atlântico-NE): uma abordagem multidisciplinar

Faria, João; Ribeiro, Pedro; Hawkins, Stephen J.; Neto, Ana I.
Fonte: Governo dos Açores - Secretaria Regional da Educação, Ciência e Cultura Publicador: Governo dos Açores - Secretaria Regional da Educação, Ciência e Cultura
Tipo: Artigo de Revista Científica
Publicado em //2013 POR
Relevância na Pesquisa
26.84%
Jornadas "Ciência nos Açores – que futuro? Tema Ciências Naturais e Ambiente", Ponta Delgada, 7-8 de Junho de 2013.; De uma forma geral, é consensual que a sobre-exploração de um dado recurso natural exerce um impacto profundo na respectiva comunidade, sobretudo quando se trata de uma espécie chave para o ecossistema. Por exemplo, as lapas que têm tradicionalmente sido exploradas para consumo humano em todos os arquipélagos da Macaronésia e, particularmente nos Açores, viram o seu efectivo populacional reduzido nas últimas décadas na grande maioria das ilhas. Alguns trabalhos recentes sugerem que a legislação existente para a sua captura tem sido ineficaz na protecção das suas populações, com óbvias implicações para a sustentabilidade das comunidades costeiras das quais fazem parte. Para fins de conservação é necessário determinar o grau de conectividade existente entre populações de lapas distribuídas ao longo de um sistema dispersivo como seja a regi.o da Macaronésia. Neste sentido, torna-se fundamental o estudo da variabilidade genética, conectividade, e resiliência das populações de lapas na Macaronésia, com especial ênfase para o arquipélago dos Açores. A conjugação de dados genéticos...

Variabilidade genética, conectividade e reiliência de populações de lapas nas ilhas da Macronésia (Atlântico-NE)

Faria, João; Ribeiro, Pedro; Neto, Ana I.
Fonte: Governo dos Açores - Secretaria Regional da Educação, Ciência e Cultura Publicador: Governo dos Açores - Secretaria Regional da Educação, Ciência e Cultura
Tipo: Conferência ou Objeto de Conferência
Publicado em //2013 POR
Relevância na Pesquisa
26.84%
Jornadas "Ciência nos Açores – que futuro? Tema Ciências Naturais e Ambiente", Ponta Delgada, 7-8 de Junho de 2013.; De uma forma geral, é consensual que a sobre-exploração de um dado recurso natural exerce um impacto profundo na respetiva comunidade, sobretudo quando se trata de uma espécie chave para a mesma. Por exemplo, as lapas que têm tradicionalmente sido exploradas para consumo humano em todos os arquipélagos da Macaronésia e particularmente nos Açores, viram o seu efetivo populacional reduzido nas últimas décadas, na grande maioria das ilhas. Alguns trabalhos recentes sugerem que a legislação existente para a sua captura tem sido ineficaz na proteção das populações deste recurso, com óbvias implicações para a sustentabilidade das comunidades costeiras das quais fazem parte. O presente projeto tem por objetivo caracterizar a variabilidade genética, conectividade, e resiliência das populações de lapas na Macaronésia, com especial foco para o arquipélago dos Açores. O principal objetivo é determinar o grau de conectividade existente entre populações de lapas distribuídas ao longo de um sistema dispersivo como seja a região da Macaronésia. Dados genéticos, biológicos, ecológicos e oceanográficos poderão contribuir de forma significativa para compreender a dinâmica populacional destes organismos...

Conservation of limpet populations: a heavily exploited resource in Azores, NE-Atlantic

Faria, João; Ribeiro, Pedro; Martins, Gustavo M.; Rivas, Manuel; Hawkins, Stephen J.; Presa, Pablo; Neto, Ana I.
Fonte: Universidade dos Açores Publicador: Universidade dos Açores
Tipo: Conferência ou Objeto de Conferência
Publicado em //2014 ENG
Relevância na Pesquisa
27.26%
10th International Temperate Reefs Symposium, The University of Western Australia, 12-17 de janeiro.; Limpet harvesting in Azores (NE Atlantic) has been taking place probably since the islands were first colonized in the XV century. Limpet species are highly exploited and populations from most islands have declined steadily bringing about catastrophic effects on coastal communities. Despite their economic importance limpets are also ecologically key species and require prioritizing conservation strategies. Patellid limpets are broadcast spawners which go through a planktonic larval stage in their life cycle. They are benthic as adults and the larva is the only phase during their life-cycle which has the ability to disperse over assumed large spatial distances. However, there is now mounting evidence that gene flow between islands and mainlands can be low, even for species with a relatively long planktonic larval stage. Low levels of larval exchange may thus limit the success of conservation objectives expected upon migration and recruitment. In dispersive isolated oceanic islands such as the Macaronesian Islands, the Azores Archipelago in particular, is not clear whether limpet populations from different islands form a single meta-population or...

Evaluation of tests based on the antibody response to keyhole limpet haemocyanin and soluble egg antigen to differentiate acute and chronic human schistosomiasis mansoni

Beck,Lílian; Van-Lüme,Daniele SM; Souza,Joelma R de; Morais,Clarice N Lins de; Melo,Wlademir G; Xavier,Edeneide; Barbosa,Constança S; Aroucha,Marcílio L; Domingues,Ana Lúcia C; Favre,Tereza; Pieri,Otávio; Abath,Frederico GC; Montenegro,Silvia ML
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2004 EN
Relevância na Pesquisa
37.1%
Specific IgG and IgM responses to soluble egg antigen (SEA) and keyhole limpet haemocyanin (KLH) were measured by ELISA in patients with acute and chronic schistosomiasis. The tests based upon IgM and IgG antibodies responses to KLH presented the best diagnostic discrimination, and can be used in conjunction with clinical and epidemiological data to the differential diagnosis of acute schistosomiasis.

Polyclonal antibodies from patients immunized with a globo H-keyhole limpet hemocyanin vaccine: Isolation, quantification, and characterization of immune responses by using totally synthetic immobilized tumor antigens

Wang, Z.-G.; Williams, L. J.; Zhang, X.-F.; Zatorski, A.; Kudryashov, V.; Ragupathi, G.; Spassova, M.; Bornmann, W.; Slovin, S. F.; Scher, H. I.; Livingston, P. O.; Lloyd, K. O.; Danishefsky, S. J.
Fonte: The National Academy of Sciences Publicador: The National Academy of Sciences
Tipo: Artigo de Revista Científica
Publicado em 14/03/2000 EN
Relevância na Pesquisa
26.84%
We have previously reported on a carbohydrate-based vaccine program for immunotherapy in cancer patients. One such vaccine, based on the globo H antigen conjugated to the protein keyhole limpet hemocyanin (KLH), has been in clinical evaluation. Although this and other carbohydrate vaccines have been shown to induce antibody responses, there are currently no quantitative data on the antibody levels achieved in immunized patients by these or other anti-cancer vaccines. We report herein an efficient route to complex synthetic oligosaccharides attached to an affinity matrix for identifying and isolating antibodies elicited against such a carbohydrate-based vaccine in humans. Pre- and postvaccination profiles from serum samples of patients immunized with globo H-KLH were compared. All anti-globo H antibody activity was efficiently separated from other serum constituents. The isolated antibodies were readily quantified, and their specificities were analyzed. Since no comparable data were available on antibodies resulting from the vaccination of other cancer patients, we compared the observed levels with those quoted in studies with bacterial polysaccharide vaccines that had been quantified. Remarkably, cancer patients immunized with globo H-KLH produce anti-globo H antibody levels often exceeding those formed by immunization with bacterial polysaccharides. In addition...

Purification and properties of α-d-mannosidase from the limpet, Patella vulgata

Snaith, Sybil M.; Levvy, G. A.; Hay, A. J.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /03/1970 EN
Relevância na Pesquisa
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1. α-Mannosidase from the limpet, Patella vulgata, was purified nearly 150-fold, with 40% recovery. β-N-Acetylglucosaminidase was removed from the preparation by treatment with ethanol. The final product was virtually free from β-galactosidase. 2. Limpet α-mannosidase was assayed at pH3.5 and at this pH it was necessary to add Zn2+ for full activity. At pH5, the enzyme had the same activity in the presence or absence of added Zn2+. 3. On incubation at acid pH, the enzyme underwent reversible inactivation, which was prevented by adding Zn2+. 4. EDTA accelerated inactivation and the addition of Zn2+ at once restored activity. No other cation was found to reactivate the enzyme. 5. Cl− had an unspecific effect on hydrolysis by limpet α-mannosidase. It increased the rate of reaction with substrate. The anion did not prevent or reverse inactivation by EDTA. 6. It is concluded that α-mannosidase is a metalloenzyme or enzyme–metal ion complex, dissociable at the pH of activity, and that it requires Zn2+ specifically.

The human primary immune response to keyhole limpet haemocyanin: interrelationships of delayed hypersensitivity, antibody response and in vitro blast transformation

Curtis, J. E.; Hersh, E. M.; Harris, J. E.; McBride, C.; Freireich, E. J.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/1970 EN
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27.1%
The immune response to a protein antigen, keyhole limpet haemocyanin, was studied in fourteen normal subjects and twenty-one patients with solid tumours. Immunological responsiveness was assessed by intracutaneous skin testing, by haemagglutinin titres and by in vitro blast transformation. No significant difference was found in the kinetics or magnitude of the immune response among subjects immunized with 0·01, 0·10, or 5·0 mg. Delayed hypersensitivity to keyhole limpet haemocyanin developed in thirty-two of thirty-four skin tested; a positive antibody titre occurred in all; and thirty-one of thirty-five had positive in vitro responses. Patients in good general condition (Group 1) had significantly greater delayed hypersensitivity and antibody responses than the normals but similar in vitro responses. All immunological parameters were depressed in the patients with advanced neoplastic disease (Group 2).

Optimized nanoscale composite behaviour in limpet teeth

Lu, Dun; Barber, Asa H.
Fonte: The Royal Society Publicador: The Royal Society
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.37%
Limpet teeth are striking examples of a biological fibrous nanocomposite consisting of goethite mineral within a polymeric chitin matrix. The mechanical function of limpet teeth is critically dependent on the efficient composite behaviour of goethite, formed as distinct discontinuous nanofibres, reinforcing the matrix. The mechanical properties of discrete volumes from a limpet tooth measured using atomic force microscopy indicate how the tooth structure can be approximated as a short fibre-reinforced composite. Short fibre composite analysis reveals how the goethite nanofibres have a length optimized for the transfer of stress from the matrix to fibre and highlight how this limpet tooth structure is efficient in a mechanical load-bearing function.

A Novel Immunomodulatory Hemocyanin from the Limpet Fissurella latimarginata Promotes Potent Anti-Tumor Activity in Melanoma

Arancibia, Sergio; Espinoza, Cecilia; Salazar, Fabián; Del Campo, Miguel; Tampe, Ricardo; Zhong, Ta-Ying; De Ioannes, Pablo; Moltedo, Bruno; Ferreira, Jorge; Lavelle, Ed C.; Manubens, Augusto; De Ioannes, Alfredo E.; Becker, María Inés
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 23/01/2014 EN
Relevância na Pesquisa
27.1%
Hemocyanins, the huge oxygen-transporting glycoproteins of some mollusks, are used as immunomodulatory proteins with proven anti-cancer properties. The biodiversity of hemocyanins has promoted interest in identifying new anti-cancer candidates with improved immunological properties. Hemocyanins promote Th1 responses without known side effects, which make them ideal for long-term sustained treatment of cancer. In this study, we evaluated a novel hemocyanin from the limpet/gastropod Fissurella latimarginata (FLH). This protein has the typical hollow, cylindrical structure of other known hemocyanins, such as the keyhole limpet hemocyanin (KLH) and the Concholepas hemocyanin (CCH). FLH, like the KLH isoforms, is composed of a single type of polypeptide with exposed N- and O-linked oligosaccharides. However, its immunogenicity was significantly greater than that of KLH and CCH, as FLH induced a stronger humoral immune response and had more potent anti-tumor activity, delaying tumor growth and increasing the survival of mice challenged with B16F10 melanoma cells, in prophylactic and therapeutic settings. Additionally, FLH-treated mice demonstrated increased IFN-γ production and higher numbers of tumor-infiltrating CD4+ lymphocytes. Furthermore...

Extreme strength observed in limpet teeth

Barber, Asa H.; Lu, Dun; Pugno, Nicola M.
Fonte: The Royal Society Publicador: The Royal Society
Tipo: Artigo de Revista Científica
Publicado em 06/04/2015 EN
Relevância na Pesquisa
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The teeth of limpets exploit distinctive composite nanostructures consisting of high volume fractions of reinforcing goethite nanofibres within a softer protein phase to provide mechanical integrity when rasping over rock surfaces during feeding. The tensile strength of discrete volumes of limpet tooth material measured using in situ atomic force microscopy was found to range from 3.0 to 6.5 GPa and was independent of sample size. These observations highlight an absolute material tensile strength that is the highest recorded for a biological material, outperforming the high strength of spider silk currently considered to be the strongest natural material, and approaching values comparable to those of the strongest man-made fibres. This considerable tensile strength of limpet teeth is attributed to a high mineral volume fraction of reinforcing goethite nanofibres with diameters below a defect-controlled critical size, suggesting that natural design in limpet teeth is optimized towards theoretical strength limits.

Keyhole limpet haemocyanin – a model antigen for human immunotoxicological studies

Swaminathan, Ashwin; Lucas, Robyn M; Dear, Keith; McMichael, Anthony J
Fonte: Wiley Publicador: Wiley
Tipo: Artigo de Revista Científica Formato: pp 1135–1142
Relevância na Pesquisa
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Immunization with a T-cell dependent antigen has been promoted as a reliable and sensitive tool for assessing the influence of putative immunotoxic exposures or agents on immune function. Keyhole limpet haemocyanin (KLH) is a very large, copper-containing protein molecule derived from the haemolymph of the inedible mollusc, Megathura crenulata. KLH is a highly immunogenic T-cell dependent antigen that is used increasingly in immunotoxicological studies, particularly in those involving animals. This report systematically reviews the human clinical studies that have used trans-cutaneous KLH immunization for assessment of the influence of various physiological and disease states and exposures on immune function over the last 20 years (1994–2013). These studies varied in their immunization protocols, formulation of KLH, dose, site and route of administration and immunoassay platforms developed to assess KLH-specific responses. KLH immunization has been well tolerated with only mild to moderate adverse effects reported. Though very promising as a model antigen candidate in immunotoxicology research, more work on standardizing immunization and immunoassay protocols is required.

Enhancing stocks of the exploited limpet Patella candei d'Orbigny via modifications in coastal engineering

Martins, Gustavo M.; Thompson, Richard C.; Neto, Ana I.; Hawkins, Stephen J.; Jenkins, Stuart R.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /01/2010 ENG
Relevância na Pesquisa
37.1%
Copyright © 2009 Elsevier Ltd. All rights reserved.; It is widely recognised that microhabitats are important for a variety of marine organisms, yet this knowledge has rarely been applied in the construction of engineered structures as a means of enhancing biodiversity or populations of species at risk. Here we examined the influence of microhabitats on the distribution and survival of the exploited limpet Patella candei on natural shores before determining the effect of introducing such habitats to an artificial seawall. On natural shores individuals were associated with pits (a natural feature of volcanic rocky shores). Animals inhabiting pits showed reduced mortality and were smaller than those on open rock. Microhabitat utilisation was similar over the vertical range of distribution of P. candei. Following observation of natural patterns, we applied this knowledge by experimentally drilling pits at varying densities and sizes in a seawall that had been constructed with simple topographical complexity. Overall, the number of animals increased in areas with experimentally increased microhabitat area. There was evidence that this was the result of immigration (larger animals) but also of increased recruitment. This study demonstrates one cost-effective way of conciliating the need to protect our coastlines while promoting the conservation and stock enhancement of over-exploited species.

Shells of the limpet Patella aspera as habitat for epibionts

Faria, João; Martins, Gustavo M.; Furtado, Miguel; Neto, Ana I.
Fonte: Sociedade Afonso Chaves Publicador: Sociedade Afonso Chaves
Tipo: Conferência ou Objeto de Conferência
Publicado em //2013 ENG
Relevância na Pesquisa
37.1%
World Congress of Malacology, Universidade dos Açores, Ponta Delgada, 21-28 de julho.; Although limpets can control the abund ance and distribution of algae and other organisms, their shells can offer a refuge for the establishment of diverse assemblage that would otherwise be excluded. In this study, we make a preliminary analysis of the epibiota growing on shells of the limpet Patella aspern. Individuals of P. aspera were collected in all islands of the Azores and the biota on their shells was identified and quantified to species leveli. A total of 144 species was identified. The composition of the epibiota assemblage varied among islands and among locations within islands. There was also significant variation in epibiota richness at the scale of locations, but not at the scale of islands. A positive and highly significant correlation was also found between epibiota richness and shell length. This study adds to the literature by showing that the shells of P. aspern, an over-exploited species in the Azores, support a very rich community of algae and invertebrates and that this is controlled by processes operating at multiple spatial scales ranging from metres (among individuals) to 100's of kilometers (among islands).

A Novel Immunomodulatory Hemocyanin from the Limpet Fissurella latimarginata Promotes Potent Anti- Tumor Activity in Melanoma

Del Campo, Miguel; Tampe, Ricardo; Espinoza, Cecilia; Zhong, Ta- Ying; Ferreira Parker, Jorge Federico; Manubens, Augusto; Becker Contreras, María Inés; Lavelle, Ed C.; De Ioannes, Pablo; Moltedo, Bruno; De Ioannes, Alfredo E.; Salazar, Fabián; Arancib
Fonte: PLOS One Publicador: PLOS One
Tipo: Artículo de revista
EN
Relevância na Pesquisa
27.1%
Artículo de publicación ISI; Hemocyanins, the huge oxygen-transporting glycoproteins of some mollusks, are used as immunomodulatory proteins with proven anti-cancer properties. The biodiversity of hemocyanins has promoted interest in identifying new anti-cancer candidates with improved immunological properties. Hemocyanins promote Th1 responses without known side effects, which make them ideal for long-term sustained treatment of cancer. In this study, we evaluated a novel hemocyanin from the limpet/gastropod Fissurella latimarginata (FLH). This protein has the typical hollow, cylindrical structure of other known hemocyanins, such as the keyhole limpet hemocyanin (KLH) and the Concholepas hemocyanin (CCH). FLH, like the KLH isoforms, is composed of a single type of polypeptide with exposed N- and O-linked oligosaccharides. However, its immunogenicity was significantly greater than that of KLH and CCH, as FLH induced a stronger humoral immune response and had more potent anti-tumor activity, delaying tumor growth and increasing the survival of mice challenged with B16F10 melanoma cells, in prophylactic and therapeutic settings. Additionally, FLH-treated mice demonstrated increased IFN-c production and higher numbers of tumor-infiltrating CD4+ lymphocytes. Furthermore...

Lower genetic diversity in the limpet Patella caerulea on urban coastal structures compared to natural rocky habitats

Fauvelot, Cecile; Bertozzi, Francesca; Costantini, Federica; Airoldi, Laura; Abbiati, Marco
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 10/11/2009
Relevância na Pesquisa
27.1%
Human-made structures are increasingly found in marine coastal habitats. The aim of the present study was to explore whether urban coastal structures can affect the genetic variation of hard-bottom species. We conducted a population genetic analysis on the limpet Patella caerulea sampled in both natural and artificial habitats along the Adriatic coast. Five microsatellite loci were used to test for differences in genetic diversity and structure among samples. Three microsatellite loci showed strong Hardy-Weinberg disequilibrium likely linked with the presence of null alleles. Genetic diversity was significantly higher in natural habitat than in artificial habitat. A weak but significant differentiation over all limpet samples was observed, but not related to the type of habitat. While the exact causes of the differences in genetic diversity deserve further investigation, these results clearly point that the expansion of urban structures can lead to genetic diversity loss at regional scales.