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O Folclore como instrumento de motivação para o ensino de Zoologia na escola; Folklore as a motivation resource for Zoology teaching in elementary school

Mendonca, Vivian Lavander
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 26/05/2008 PT
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O ensino de Zoologia na escola básica é a preocupação do presente trabalho, que enfatiza o diálogo entre o meio científico e o educacional. Com base em fundamentação teórica sobre interdisciplinaridade, natureza do conhecimento científico e do conhecimento popular na forma de folclore, desenvolveu-se a proposta deste trabalho, que tem como idéia central utilizar elementos do folclore como instrumento de motivação dos alunos no estudo de um grupo animal. A escolha do grupo animal recaiu nos anfíbios, muito presentes no folclore. Para essa finalidade, optou-se pela elaboração de um texto sobre anfíbios que alia os dois campos do saber, levando em conta a questão da transposição didática de conceitos científicos e as características estruturais de um texto didático. O principal produto desse trabalho é o texto "O Mundo dos Anfíbios - Folclore e Ciência", que sugere uma possibilidade na construção de significados na rede de conhecimentos, usando como elemento de motivação a abordagem interdisciplinar.; The teaching of Zoology in elementary schools is the preoccupancy of this work, in which the dialogue between scientific and educational fields is emphasized. With the support of theoretical fundamentality on interdisciplinarity ...

Conhecimento, atitude e pratica relacionados a sexualidade de mulheres tratadas de cancer genital

Milzen Jessel Lavander Giatti
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 31/08/2005 PT
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Objetivos - estudar alguns aspectos relacionados à sexualidade e avaliar o perfil sódio-demográfico de mulheres tratadas de câncer genital e compará-las com um grupo de mulheres sem câncer. Sujeitos e métodos - estudo de corte transversal, comparativo, tipo inquérito CAP ? Conhecimento, Atitude e Prática. No período de julho de 2002 a março de 2004, 260 mulheres foram entrevistadas, 130 anteriormente tratadas de câncer ginecológico e 130 sem antecedente de câncer, pareadas por idade e por domicílio. Foi idealizado um questionário estruturado, com perguntas pré-codificadas e abertas, cujas respostas foram armazenadas num banco de dados, utilizando-se o software EPI-INFO 6,04b. Para o processamento e análise das informações foram construídas tabelas de contingência com freqüências absolutas e relativas. A associação entre as variáveis foi avaliada através dos testes de qui-quadrado de Pearson ou exato de Fisher. Para a análise do conhecimento e da atitude criaram-se indicadores através de média aritmética das respostas corretas, cujos valores foram comparados na variável dependente através do teste de Mann-Whitney, e a diferença estatística de acertos de cada questão estudada através do teste exato de Fisher. Resultados - A idade média das mulheres foi de 56...

Remoção de compostos nitrogenados e fosfatados de efluentes por meio de reator anaeróbico com fluxo ascendente

Costa,Weruska de Melo; Lavander,Henrique David; Oliveira,Leônidas; Souza,André Batista de; Gálvez,Alfredo Olivera; Arana,Luiz Alejandro Vinatea
Fonte: Embrapa Informação Tecnológica; Pesquisa Agropecuária Brasileira Publicador: Embrapa Informação Tecnológica; Pesquisa Agropecuária Brasileira
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2013 PT
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O objetivo deste trabalho foi avaliar a remoção de compostos nitrogenados e fosfatados e da carga orgânica poluidora oriunda de cultivo heterotrófico de camarão marinho, por meio de reator anaeróbico. Foi construído um reator com fluxo ascendente, em que os efluentes de entrada e saída foram avaliados continuamente durante 120 horas, com três repetições. Os parâmetros físicos e químicos avaliados foram: temperatura, pH, condutividade, ortofosfato, nitrito, nitrato, amônia, demanda química de oxigênio e sólidos totais. O reator permaneceu estável, com boas condições de retenção de sólidos. O reator anaeróbico removeu 96,7% do nitrogênio amoniacal e 91% de ortofosfatos dos efluentes de cultivo de camarão marinho, o que mostra que os efluentes tratados estão dentro dos limites estabelecidos pela legislação.

Proteolytic Cleavage of the FlhB Homologue YscU of Yersinia pseudotuberculosis Is Essential for Bacterial Survival but Not for Type III Secretion

Lavander, Moa; Sundberg, Lena; Edqvist, Petra J.; Lloyd, Scott A.; Wolf-Watz, Hans; Forsberg, Åke
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /08/2002 EN
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Pathogenic Yersinia species employ a type III secretion system (TTSS) to target antihost factors, Yop proteins, into eukaryotic cells. The secretion machinery is constituted of ca. 20 Ysc proteins, nine of which show significant homology to components of the flagellar TTSS. A key event in flagellar assembly is the switch from secreting-assembling hook substrates to filament substrates, a switch regulated by FlhB and FliK. The focus of this study is the FlhB homologue YscU, a bacterial inner membrane protein with a large cytoplasmic C-terminal domain. Our results demonstrate that low levels of YscU were required for functional Yop secretion, whereas higher levels of YscU lowered both Yop secretion and expression. Like FlhB, YscU was cleaved into a 30-kDa N-terminal and a 10-kDa C-terminal part. Expression of the latter in a wild-type strain resulted in elevated Yop secretion. The site of cleavage was at a proline residue, within the strictly conserved amino acid sequence NPTH. A YscU protein with an in-frame deletion of NPTH was cleaved at a different position and was nonfunctional with respect to Yop secretion. Variants of YscU with single substitutions in the conserved NPTH sequence—i.e., N263A, P264A, or T265A—were not cleaved but retained function in Yop secretion. Elevated expression of these YscU variants did...

YscP and YscU Regulate Substrate Specificity of the Yersinia Type III Secretion System

Edqvist, Petra J.; Olsson, Jan; Lavander, Moa; Sundberg, Lena; Forsberg, Åke; Wolf-Watz, Hans; Lloyd, Scott A.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /04/2003 EN
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Pathogenic Yersinia species use a type III secretion system to inhibit phagocytosis by eukaryotic cells. At 37°C, the secretion system is assembled, forming a needle-like structure on the bacterial cell surface. Upon eukaryotic cell contact, six effector proteins, called Yops, are translocated into the eukaryotic cell cytosol. Here, we show that a yscP mutant exports an increased amount of the needle component YscF to the bacterial cell surface but is unable to efficiently secrete effector Yops. Mutations in the cytoplasmic domain of the inner membrane protein YscU suppress the yscP phenotype by reducing the level of YscF secretion and increasing the level of Yop secretion. These results suggest that YscP and YscU coordinately regulate the substrate specificity of the Yersinia type III secretion system. Furthermore, we show that YscP and YscU act upstream of the cell contact sensor YopN as well as the inner gatekeeper LcrG in the pathway of substrate export regulation. These results further strengthen the strong evolutionary link between flagellar biosynthesis and type III synthesis.

A Conserved α-Helix Essential for a Type VI Secretion-Like System of Francisella tularensis▿ †

Bröms, Jeanette E.; Lavander, Moa; Sjöstedt, Anders
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
EN
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Francisella tularensis harbors genes with similarity to genes encoding components of a type VI secretion system (T6SS) recently identified in several gram-negative bacteria. These genes include iglA and iglB encoding IglA and IglB, homologues of which are conserved in most T6SSs. We used a yeast two-hybrid system to study the interaction of the Igl proteins of F. tularensis LVS. We identified a region of IglA, encompassing residues 33 to 132, necessary for efficient binding to IglB, as well as for IglAB protein stability and intramacrophage growth. In particular, residues 103 to 122, overlapping a highly conserved α-helix, played an absolutely essential role. Point mutations within this domain caused modest defects in IglA-IglB binding in the yeast Saccharomyces cerevisiae but markedly impaired intramacrophage replication and phagosomal escape, resulting in severe attenuation of LVS in mice. Thus, IglA-IglB complex formation is clearly crucial for Francisella pathogenicity. This interaction may be universal to type VI secretion, since IglAB homologues of Yersinia pseudotuberculosis, Pseudomonas aeruginosa, Vibrio cholerae, Salmonella enterica serovar Typhimurium, and Escherichia coli were also shown to interact in yeast, and the interaction was dependent on preservation of the same α-helix. Heterologous interactions between nonnative IglAB proteins further supported the notion of a conserved binding site. Thus...

Autoproteolysis of YscU of Yersinia pseudotuberculosis Is Important for Regulation of Expression and Secretion of Yop Proteins ▿

Björnfot, Ann-Catrin; Lavander, Moa; Forsberg, Åke; Wolf-Watz, Hans
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
EN
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YscU of Yersinia can be autoproteolysed to generate a 10-kDa C-terminal polypeptide designated YscUCC. Autoproteolysis occurs at the conserved N↓PTH motif of YscU. The specific in-cis-generated point mutants N263A and P264A were found to be defective in proteolysis. Both mutants expressed and secreted Yop proteins (Yops) in calcium-containing medium (+Ca2+ conditions) and calcium-depleted medium (−Ca2+ conditions). The level of Yop and LcrV secretion by the N263A mutant was about 20% that of the wild-type strain, but there was no significant difference in the ratio of the different secreted Yops, including LcrV. The N263A mutant secreted LcrQ regardless of the calcium concentration in the medium, corroborating the observation that Yops were expressed and secreted in Ca2+-containing medium by the mutant. YscF, the type III secretion system (T3SS) needle protein, was secreted at elevated levels by the mutant compared to the wild type when bacteria were grown under +Ca2+ conditions. YscF secretion was induced in the mutant, as well as in the wild type, when the bacteria were incubated under −Ca2+ conditions, although the mutant secreted smaller amounts of YscF. The N263A mutant was cytotoxic for HeLa cells, demonstrating that the T3SS-mediated delivery of effectors was functional. We suggest that YscU blocks Yop release and that autoproteolysis is required to relieve this block.

The Role of the Francisella Tularensis Pathogenicity Island in Type VI Secretion, Intracellular Survival, and Modulation of Host Cell Signaling

Bröms, Jeanette E.; Sjöstedt, Anders; Lavander, Moa
Fonte: Frontiers Research Foundation Publicador: Frontiers Research Foundation
Tipo: Artigo de Revista Científica
Publicado em 21/12/2010 EN
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Francisella tularensis is a highly virulent gram-negative intracellular bacterium that causes the zoonotic disease tularemia. Essential for its virulence is the ability to multiply within host cells, in particular monocytic cells. The bacterium has developed intricate means to subvert host immune mechanisms and thereby facilitate its intracellular survival by preventing phagolysosomal fusion followed by escape into the cytosol, where it multiplies. Moreover, it targets and manipulates numerous host cell signaling pathways, thereby ameliorating the otherwise bactericidal capacity. Many of the underlying molecular mechanisms still remain unknown but key elements, directly or indirectly responsible for many of the aforementioned mechanisms, rely on the expression of proteins encoded by the Francisella pathogenicity island (FPI), suggested to constitute a type VI secretion system. We here describe the current knowledge regarding the components of the FPI and the roles that have been ascribed to them.

IglG and IglI of the Francisella Pathogenicity Island Are Important Virulence Determinants of Francisella tularensis LVS ▿ †

Bröms, Jeanette E.; Lavander, Moa; Meyer, Lena; Sjöstedt, Anders
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /09/2011 EN
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The Gram-negative bacterium Francisella tularensis is the causative agent of tularemia, a disease intimately associated with the multiplication of the bacterium within host macrophages. This in turn requires the expression of Francisella pathogenicity island (FPI) genes, believed to encode a type VI secretion system. While the exact functions of many of the components have yet to be revealed, some have been found to contribute to the ability of Francisella to cause systemic infection in mice as well as to prevent phagolysosomal fusion and facilitate escape into the host cytosol. Upon reaching this compartment, the bacterium rapidly multiplies, inhibits activation of the inflammasome, and ultimately causes apoptosis of the host cell. In this study, we analyzed the contribution of the FPI-encoded proteins IglG, IglI, and PdpE to the aforementioned processes in F. tularensis LVS. The ΔpdpE mutant behaved similarly to the parental strain in all investigated assays. In contrast, ΔiglG and ΔiglI mutants, although they were efficiently replicating in J774A.1 cells, both exhibited delayed phagosomal escape, conferred a delayed activation of the inflammasome, and exhibited reduced cytopathogenicity as well as marked attenuation in the mouse model. Thus...

DotU and VgrG, Core Components of Type VI Secretion Systems, Are Essential for Francisella LVS Pathogenicity

Bröms, Jeanette E.; Meyer, Lena; Lavander, Moa; Larsson, Pär; Sjöstedt, Anders
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 13/04/2012 EN
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The Gram-negative bacterium Francisella tularensis causes tularemia, a disease which requires bacterial escape from phagosomes of infected macrophages. Once in the cytosol, the bacterium rapidly multiplies, inhibits activation of the inflammasome and ultimately causes death of the host cell. Of importance for these processes is a 33-kb gene cluster, the Francisella pathogenicity island (FPI), which is believed to encode a type VI secretion system (T6SS). In this study, we analyzed the role of the FPI-encoded proteins VgrG and DotU, which are conserved components of type VI secretion (T6S) clusters. We demonstrate that in F. tularensis LVS, VgrG was shown to form multimers, consistent with its suggested role as a trimeric membrane puncturing device in T6SSs, while the inner membrane protein DotU was shown to stabilize PdpB/IcmF, another T6SS core component. Upon infection of J774 cells, both ΔvgrG and ΔdotU mutants did not escape from phagosomes, and subsequently, did not multiply or cause cytopathogenicity. They also showed impaired activation of the inflammasome and marked attenuation in the mouse model. Moreover, all of the DotU-dependent functions investigated here required the presence of three residues that are essentially conserved among all DotU homologues. Thus...

Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection

Bröms, Jeanette E.; Meyer, Lena; Sun, Kun; Lavander, Moa; Sjöstedt, Anders
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 20/11/2012 EN
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Gram-negative bacteria have evolved sophisticated secretion machineries specialized for the secretion of macromolecules important for their life cycles. The Type VI secretion system (T6SS) is the most widely spread bacterial secretion machinery and is encoded by large, variable gene clusters, often found to be essential for virulence. The latter is true for the atypical T6SS encoded by the Francisella pathogenicity island (FPI) of the highly pathogenic, intracellular bacterium Francisella tularensis. We here undertook a comprehensive analysis of the intramacrophage secretion of the 17 FPI proteins of the live vaccine strain, LVS, of F. tularensis. All were expressed as fusions to the TEM β-lactamase and cleavage of the fluorescent substrate CCF2-AM, a direct consequence of the delivery of the proteins into the macrophage cytosol, was followed over time. The FPI proteins IglE, IglC, VgrG, IglI, PdpE, PdpA, IglJ and IglF were all secreted, which was dependent on the core components DotU, VgrG, and IglC, as well as IglG. In contrast, the method was not directly applicable on F. novicida U112, since it showed very intense native β-lactamase secretion due to FTN_1072. Its role was proven by ectopic expression in trans in LVS. We did not observe secretion of any of the LVS substrates VgrG...

Screening for Inhibition of Vibrio cholerae VipA-VipB Interaction Identifies Small-Molecule Compounds Active against Type VI Secretion

Sun, Kun; Bröms, Jeanette; Lavander, Moa; Gurram, Bharat Kumar; Enquist, Per-Anders; Andersson, C. David; Elofsson, Mikael; Sjöstedt, Anders
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /07/2014 EN
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The type VI secretion system (T6SS) is the most prevalent bacterial secretion system and an important virulence mechanism utilized by Gram-negative bacteria, either to target eukaryotic cells or to combat other microbes. The components show much variability, but some appear essential for the function, and two homologues, denoted VipA and VipB in Vibrio cholerae, have been identified in all T6SSs described so far. Secretion is dependent on binding of an α-helical region of VipA to VipB, and in the absence of this binding, both components are degraded within minutes and secretion is ceased. The aim of the study was to investigate if this interaction could be blocked, and we hypothesized that such inhibition would lead to abrogation of T6S. A library of 9,600 small-molecule compounds was screened for their ability to block the binding of VipA-VipB in a bacterial two-hybrid system (B2H). After excluding compounds that showed cytotoxicity toward eukaryotic cells, that inhibited growth of Vibrio, or that inhibited an unrelated B2H interaction, 34 compounds were further investigated for effects on the T6SS-dependent secretion of hemolysin-coregulated protein (Hcp) or of phospholipase A1 activity. Two compounds, KS100 and KS200, showed intermediate or strong effects in both assays. Analogues were obtained...

Análise química e biológica de metabólitos voláteis isolados de plantas das famílias Myrtaceae e Zingiberaceae

Ribeiro, Jane Cristina Lara
Fonte: Curitiba Publicador: Curitiba
Tipo: Dissertação de Mestrado
POR
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In this work we investigated the chemical composition of volatile metabolites extracted from four plants Pepper pseudocaryophyllus (Myrtaceae), Psidium cattleyanum (Myrtaceae), Hedychium coronarium (Zingiberaceae) and Costus spiralis (Zingiberaceae) by gas chromatography-mass spectrometry (GC / MS). Results are presented relative to seasonal chemical composition of the essential oil obtained from the leaves of Pepper pseudocaryophyllus in samples collected bimonthly from January to November 2009. In November showed the highest concentrations on the major constituents of the essential oil from the leaves of this species, being chavibetol (70.90%) and methyleugenol (20.70%). Furthermore, we evaluated the antimicrobial activity of essential oil from the leaves of P. pseudocaryophyllus through techniques of diffusion disk and broth microdilution. The chavibetol, major compound was shown to be responsible for the greatest antifungal activity. We present also the seasonality of the essential oil obtained from the rhizome of Hedychium coronarium in samples collected bimonthly from March to January 2012-2013. In March the highest concentrations were observed on the major constituents of the essential oil of the rhizome of this species, and β-pinene (31.82%) and 1...

Dise??o e implementaci??n de un sistema de indicadores de gesti??n en la Lavander??a Classic Dry Cleaning?? Bogot?? bajo la metodolog??a del Balanced Scorecard

Urrea Cardona, Daniela
Fonte: Universidad EAFIT; Ingenier??a de Procesos; Escuela de Ingenier??a. Departamento de Ingenier??a Procesos Publicador: Universidad EAFIT; Ingenier??a de Procesos; Escuela de Ingenier??a. Departamento de Ingenier??a Procesos
Tipo: bachelorThesis; Trabajo de grado; acceptedVersion
SPA
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Diálogos de la comunicación

Fonte: Lima; Perú; Federación Latinoamericana de Asociaciones de Facultades de Comunicación Social (FELAFACS); Cuatrimestral; 35; 3; En curso; Diálogos de la comunicación; 1987 Publicador: Lima; Perú; Federación Latinoamericana de Asociaciones de Facultades de Comunicación Social (FELAFACS); Cuatrimestral; 35; 3; En curso; Diálogos de la comunicación; 1987
Tipo: REVISTA Formato: Impresa
ES
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Editorial : Diálogos; Ensayo: La nueva cara de nuestras radios en estos tiempos neoliberales : José Ignacio López Vigil; Ensayo: La radio: Una relación comunicativa : María Cristina Mata; Ensayo: Radio popular en Bolivia: la lucha de obreros y campesinos para democratizar la comunicación : Luis Ramiro Beltrán y Jaime Reyes; Ensayo: Recepción y consumo radial, una perspectiva desde los sujetos : Sandro Macassi Lavander; Ensayo: La voz de la mixteca . Diagnóstico y perspectivas : Inés Cornejo Portugal; Ensayo: Circuito interno de audi: Un desafío para la integración de la empresa moderna : André Barbosa Filbo; Investigación: Conceptualización y supervisión de la comunicación y el compromiso organizacional : Fderico Varona Madrid; Enseñanza: ¿Qué significa hablar de radio? : Gina Gogin; Enseñanza: La tensión teoría - práctica en la enseñanza de la comunicación : Carlos E. Luna Cortés; Epistolae : Diálogos; Noticias : Diálogos; Federación : Diálogos; Libros : Diálogos

The Twin Arginine Translocation System Is Essential for Virulence of Yersinia pseudotuberculosis

Lavander, Moa; Ericsson, Solveig K.; Bröms, Jeanette E.; Forsberg, Åke
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /03/2006 EN
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Yersinia species pathogenic to humans have been extensively characterized with respect to type III secretion and its essential role in virulence. This study concerns the twin arginine translocation (Tat) pathway utilized by gram-negative bacteria to secrete folded proteins across the bacterial inner membrane into the periplasmic compartment. We have shown that the Yersinia Tat system is functional and required for motility and contributes to acid resistance. A Yersinia pseudotuberculosis mutant strain with a disrupted Tat system (tatC) was, however, not affected in in vitro growth or more susceptible to high osmolarity, oxidative stress, or high temperature, nor was it impaired in type III secretion. Interestingly, the tatC mutant was severely attenuated via both the oral and intraperitoneal routes in the systemic mouse infection model and highly impaired in colonization of lymphoid organs like Peyer's patches and the spleen. Our work highlights that Tat secretion plays a key role in the virulence of Y. pseudotuberculosis.

Rapports entre l'évolution florale d'un lavandin et l'essence produite; Relationship between the floral evolution of a lavandin and the oil produced

Barbier, M.E.C.
Fonte: INRA - Instituto Nacional de Investigação Agronômica da França Publicador: INRA - Instituto Nacional de Investigação Agronômica da França
Tipo: Journal Article-postprint
FR; FRENCH
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Au cours de recherches sur les rapports existant entre le Lavandin et l'Abeille, nous avons été conduits à examiner en rapport avec l'évolution florale de la plante, les évolutions du rendement et de l'indice de réfraction des essences obtenues par des distillations successives. Ces travaux ont porté sur la variété de Lavandin "Z" ·sélectionnée au Domaine de Puberclaire (B.-A.), et cultivée au Laboratoire Apicole de Nice (A.-M.). Les 200 plants mis en place en 1951 ont atteint au moment des essais en 1957 leur plein développement.

OCCUPACIONAL EXPOSURE WITH POTENTIALLY HAZARDOUS BIOLOGICAL MATERIAL AMONG WORKERS IN HOSPITAL SERVICES; EXPOSIÇÃO OCUPACIONAL COM MATERIAL POTENCIALMENTE CONTAMINADO ENTRE PROFISSIONAIS DA ÁREA DE APOIO; EXPOSICIÓN OCUPACIONAL CON MATERIAL POTENCIALMENTE CONTAMINADO ENTRE PROFESIONALES DEL ÁREA DE APOYO

Morais, Natasha de Oliveira; Paniago, Anamaria Melo Miranda; Negri, Adriana Carla; de Oliveira, Olcinei Alves; da Cunha, Rivaldo Venâncio; de Oliveira, Sandra Maria do Valle Leone
Fonte: Universidade Federal do Paraná Publicador: Universidade Federal do Paraná
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 14/12/2009 POR
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Investigación descriptiva, cuantitativa, que se propuso a levantar la frecuencia de accidentes ocupacionales,envolviendo materiales biológicos potencialmente contaminados, que ocurrieron entre los profesionales que actúan en unhospital de enseñanza y caracterizar aquellos acometieron el equipo de higienización y limpieza y lavandería. Fueronanalizados 238 informes de expedientes de accidentes de trabajo del Servicio de Epidemiología entre 2004-2009. De estos,10.9% (26/239) correspondían a accidentes ocurridos con profesionales de la limpieza hospitalaria, 2.5% de profesionalesde la lavandería. Fue predominante el sexo femenino, faja etaria de 30-40 años, del tipo percutáneo, destacándose losquirodáctilos como área corporal más afectada y el líquido potencialmente contaminado fue la sangre. Tres grandes pilaresde ayuda deben ser fortalecidos: el sistema de información para subsidiar la política institucional, la formación continuadade los profesionales que generan residuos perforadores y la compra de materiales con dispositivos de seguridad.; This is a descriptive and quantitative research which aimed to study the annual frequency of workplaceaccidents that occurred among health professionals and to characterize the occurrence of work acidentes involvinghospital hygiene staff. 238 professional accidents’ reports were analyzed...