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Karyotype variability in KP1(+) and KP1(-) strains of Trypanosoma rangeli isolated in Brazil and Colombia

CABRINE-SANTOS, Marlene; FERREIRA, Keila A. M.; TOSI, Luiz R. O.; LAGES-SILVA, Eliane; RAMIREZ, Luis Eduardo; PEDROSA, Andre Luiz
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artigo de Revista Científica
ENG
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In the present study, the molecular karyotypes of 12 KP1(+) and KP1(-) Trypanosoma rangeli strains were determined and 10 different molecular markers were hybridized to the chromosomes of the parasite, including seven obtained from T. rangeli [ubiquitin hydrolase (UH), a predicted serine/threonine protein kinase (STK), hexose transporter, hypothetical protein, three anonymous sequences] and three from Trypanosoma cruzi [ubiquitin-conjugating enzyme E2 (UBE2), ribosomal RNA methyltransferase (rRNAmtr), proteasome non-ATPase regulatory subunit 6 (PSMD6)]. Despite intraspecific variation, analysis of the karyotype profiles permitted the division of the T rangeli strains into two groups coinciding with the KP1(+) and KP1(-) genotypes. Southern blot hybridization showed that, except for the hexose transporter probe, all other probes produced distinct patterns able to differentiate the KP1(+) and KP1(-) genotypes. The UH, STK and An-1A04 probes exclusively hybridized to the chromosomes of KP1(+) strains and can be used as markers of this group. In addition, the UBE2, rRNAmtr and PSMD6 markers, which are present in a conserved region in all trypanosomatid species sequenced so far, co-hybridized to the same T. rangeli chromosomal bands, suggesting the occurrence of gene synteny in these species. The finding of distinct molecular karyotypes in KP1(+) and KP1 (-) strains of T rangeli is noteworthy and might be used as a new approach to the study of genetic variability in this parasite. Together with the Southern blot hybridization results...

Cariótipo molecular: uma ferramenta para o estudo analítico e evolutivo do genoma de Trypanosoma cruzi; Molecular karyotype: a tool for the analytical and evolutionary study of Trypanosoma cruzi genome.

Pedroso Junior, Aurelio
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 20/01/2005 PT
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Diferentes métodos de caracterização e inferências filogenéticas baseadas na seqüência de alguns genes nucleares indicam que Typanosoma cruzi pode ser dividido em dois grupos principais, denominados T. cruzi I e T. cruzi II. Outros subgrupos de isolados foram descritos, tais como grupo de rDNA 1/2 e zimodema 3 (Z3) cuja relação filogenética com os grupos T.cruzi I e T.cruzi II ainda não está clara. O cariótipo molecular é uma ferramenta que permite abordar diferentes aspectos do genoma de organismos. Neste trabalho, caracterizamos o cariótipo molecular de 22 isolados de T. cruzi a partir da separação de seus cromossomos por eletroforese de campo pulsado e hibridação com sondas que representam genes codificadores de proteína e de RNA. Verificamos extenso polimorfismo cromossômico entre os isolados e que isolados pertencentes aos grupos T. cruzi II, rDNA 1/2 e Z3 têm cromossomos de tamanho molecular maior (até 3,5 Mb) em relação a isolados de T. cruzi I (até 2,8 Mb). Os dados do cariótipo molecular também foram utilizados para averiguar o significado evolutivo do polimorfismo cromossômico. As análises fenéticas foram baseadas no índice de diferença absoluta de tamanho cromossômico (aCSDI) obtido a partir da hibridação dos cromossomos com um número variável de marcadores genéticos. Inicialmente analisamos nove isolados...

Karyotype differentiation patterns in species of the subfamily Scarabaeinae (Scarabaeidae, Coleoptera)

Cabral-de-Mello, Diogo Cavalcanti; de Oliveira, Sarah Gomes; Ramos, Ituza Celeste; de Moura, Rita de Cassia
Fonte: Pergamon-Elsevier B.V. Ltd Publicador: Pergamon-Elsevier B.V. Ltd
Tipo: Artigo de Revista Científica Formato: 1243-1250
ENG
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); The aim of this study was to describe the karyotype of species belonging to the subfamily Scarabaeinae (Coleoptera, Scarabaeidae) and to compile the conventional cytogenetic data available in the literature for this group. The karyotypes of ten species belonging to the tribes Canthonini, Coprini, Onthophagini and Phanaeini were analyzed by conventional staining. Eight of these species were described for the first time (Canthon aff carbonarius, Canthon chalybaeus, Coprophanaeus dardanus, Deltochilum aff amazonicum, Dichotomius geminatus, Oxysternon silenus, Phanaeus chalcomelas and Malagoniella aff astyanax) and two were redescribed (Diabroctis mimas and Digitonthophagus gazella) since their karyotypes differed from those previously published in the literature. Four species studied showed a diploid number of 2n = 20 and a parachute type sex determining system and the karyotype was 2n = 20,Xy in two species and 2n = 18,Xy(p), 2n = 19,X0,2n = 12,XY and 2n = 14,neoXY in one each. The chromosome morphology of the different species varied, with the observation of metacentric, submetacentric, subacrocentric and acrocentric chromosomes. The X chromosome was predominantly meta or submetacentric in the species analyzed...

A new karyotype of Calomys (Rodentia, Sigmodontinae)

Lima, J. Fernando de S.; Kasahara, Sanae
Fonte: Fundação Zoobotânica do Rio Grande do Sul Publicador: Fundação Zoobotânica do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: 133-136
ENG
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); The genus Calomys Waterhouse, 1837 is widely distributed within South America, being found in Venezuela, Colombia, Peru, Bolivia, Brazil, Paraguay, Uruguay and Argentina. Specimens of Calomys were collected in Formoso do Araguaia, Tocantins, Brazil. For chromosome characterization standard staining techniques and as G-banding and nucleolar organizer region were used. The karyotype was 2n=46 and AN=66. The X chromosome is a medium metacentric and the Y chromosome a small acrocentric chromosome. Chromosome homologies with other species were observed. Probably, karyotype differences were basically due to Robertsonian rearrangements.

Karyotype description of two Neotropical Psittacidae species: the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and the Hawk-headed Parrot, Deroptyus accipitrinus (Psittaciformes: Aves), and its significance for conservation plans

Lunardi, Vitor de Oliveira; Francisco, Mercival Roberto; Rocha, Guaracy Tadeu; Goldschmidt, Beatriz; Galetti Junior, Pedro Manoel
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: 283-287
ENG
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Neotropical parrots are among the most threatened groups of birds in the world, and many species are facing extinction in a near future. At the same time, the taxonomic position of many species remains unclear. Karyotype analysis has been used to elucidate the phylogenetic status of many bird groups, also providing important information for both in situ and ex situ conservation plans. The objective of the present study was to describe for the first time the karyotypes of the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and of the Hawk-headed Parrot, Deroptyus accipitrinus. A diploid number of 2n = 70 and a karyotype similar to the main pattern previously found for the genera Ara, Cyanopsitta, Aratinga, Propyrrhura, Pionites, Pionopsitta, Nandayus, and Guaruba were found for both species. These karyotype descriptions can be a starting point for the genetic monitoring of these two declining species.

Karyotype analysis in Brachiaria (Poaceae) species

Bernini, C.; Marin-Morales, M. A.
Fonte: Faculty Press Publicador: Faculty Press
Tipo: Artigo de Revista Científica Formato: 157-171
ENG
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This is the first karyotype characterization of Brachiaria species. Twelve accessions belonging to five species were analysed. The basic chromosome number was x = 9 and 7, the same reported for the tribe Paniceae. Variations in the chromosome number were observed in B. decumbens (2n = 18; 36) and B. humidicola (20 = 36; 42; 54). Chromosome numbers of 20 = 18 in B. ruziziensis and 20 = 36 in B. brizantha and B. jubata were recorded. Inter- and intraspecific karyotype differentiation of the accessions analysed was facilitated by variations in karyotypic symmetry. The karyotypes were generally considered symmetrical, with a tendency to asymmetry in the direction of the polyploids. It is suggested that addition, deletions and mainly polyploidy have been the most direct causes involved in the chromosome evolution of this genus.

Karyotype studies of some species of Dalechampia plum. (Euphorbiaceae)

Vanzela, A. L. L.; Ruas, P. M.; Marin-Morales, M. A.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 25-33
ENG
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Karyotype studies in eight species of Dalechampia, including 10 natural populations, revealed chromosome numbers (2n = 36, 46, 138 and 198) differing from two numbers cited in the literature (2n = 44 and 72). The basic number x = 6, as in the genus Acalypha, may be considered ancestral in Dalechampia. Analysis of Chromosome number, haploid chromosome length and karyotype symmetry suggests that the major chromosome mechanism acting in karyotype evolution of Dalechampia is polyploidy, but differences in chromosome morphology may be caused by chromosome rearrangements.

A new karyotype of oligoryzomys (sigmodontinae, rodentia) from central Brazil

De S. Lima, J. Fernando; Bonvicino, Cibele R.; Kasahara, Sanae
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 1-6
ENG
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); A new karyotype of the genus Oligoryzomys was described for specimens collected in Brazilian Cerrado in Tocantins and Goias States. Conventional staining, G-, C-banding, and Ag-NOR staining techniques were used for describe this karyotype with 2n = 70, AN = 74 or 76. The chromosome complement, with the highest diploid number known among Oligoryzomys species, differs from all others previously reported. This small sized Oligoryzomys species can be differentiated from other Brazilian Oligoryzomys not only by chromosomal complement, but also in some morphological attributes. The new species is apparently restricted in distribution and endemic of Brazilian Cerrado, occurring only in Rio Tocantins basin.

Description of the nucleolar activity and karyotype in germinative cell lines of Rhodnius domesticus (Triatominae, Heteroptera)

Morielle, Alessandra; Oliveira, Maria Tercília Vilela de Azeredo
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 31-37
ENG
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The purpose of this work was to study the karyotype, spermatogenesis and nucleolar activity at meiosis, in the species Rhodnius domesticus (Heteroptera, Triatominae). The testicular tubules were cytologically prepared by the conventional method of cell crushing and subsequent application of cytogenetic staining techniques with lacto-acetic orcein and silver-ion impregnation. The species under study presented karyotype 2n= 20A+XY, the modal number of the subfamily Triatominae. The chromosomes presented no primary constriction and were therefore characterized as holocentric. It was observed that the sex chromosomes sometimes were located at the periphery, close to the ring formed by autosomes, at first meiotic division. At metaphases II, sex chromosomes were positioned in the center of the autosomal ring, thus evidencing a postreductional behavior. These same chromosomes showed late migration at anaphases and were clearly impregnated with silver-ions, suggesting they bore Nucleolar Organizer Regions. Dispersed nucleolar corpuscles in cytoplasm until telophase II and small dots in spermatids strongly impregnated with silver, could be seen. Thus, it may be inferred that, in triatomines, the nucleolus does not completely disappear but remains in the form of small corpuscles that have a role in cell differentiation.

Chromosomal characteristics and karyotype evolution of oxyopidae spiders (araneae, entelegynae)

Stávale, L. M.; Schneider, M. C.; Brescovit, A. D.; Cella, D. M.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 752-763
ENG
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We made a cytogenetic analysis of four species of Oxyopidae and compared it with the karyotype data of all species of this family. In Hamataliwa sp, the mitotic cells showed 2n♂ = 26+X 1X 2 and telocentric chromosomes. The 2n♂ = 28, which has been described for only one oxyopid spider, is the highest diploid number reported for this family. Peucetia species exhibited distinct karyotype characteristics, i.e., 2n♂ = 20+X 1X 2 in P. flava and 2n♂ = 20+X in P. rubrolineata, revealing interspecific chromosome variability within this genus. However, both Peucetia species exhibited telocentric chromosomes. The most unexpected karyotype was encountered in Oxyopes salticus, which presented 2n♂ = 10+X in most individuals and a predominance of biarmed chromosomes. Additionally, one male of the sample of O. salticus was heterozygous for a centric fusion that originated the first chromosomal pair and exhibited one supernumerary chromosome in some cells. Testicular nuclei of Hamataliwa sp and O. salticus revealed NORs on autosomal pairs, after silver impregnation. The majority of Oxyopidae spiders have their karyotype differentiated by both reduction in diploid number chromosome number and change of the sex chromosome system to X type; however...

Molecular karyotype and chromosomal localization of genes encoding ß-tubulin, cysteine proteinase, hsp 70 and actin in Trypanosoma rangeli

Toaldo,CB; Steindel,M; Sousa,MA; Tavares,CC
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2001 EN
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The molecular karyotype of nine Trypanosoma rangeli strains was analyzed by contour-clamped homogeneous electric field electrophoresis, followed by the chromosomal localization of ß-tubulin, cysteine proteinase, 70 kDa heat shock protein (hsp 70) and actin genes. The T. rangeli strains were isolated from either insects or mammals from El Salvador, Honduras, Venezuela, Colombia, Panama and southern Brazil. Also, T. cruzi CL-Brener clone was included for comparison. Despite the great similarity observed among strains from Brazil, the molecular karyotype of all T. rangeli strains analyzed revealed extensive chromosome polymorphism. In addition, it was possible to distinguish T. rangeli from T. cruzi by the chromosomal DNA electrophoresis pattern. The localization of ß-tubulin genes revealed differences among T. rangeli strains and confirmed the similarity between the isolates from Brazil. Hybridization assays using probes directed to the cysteine proteinase, hsp 70 and actin genes discriminated T. rangeli from T. cruzi, proving that these genes are useful molecular markers for the differential diagnosis between these two species. Numerical analysis based on the molecular karyotype data revealed a high degree of polymorphism among T. rangeli strains isolated from southern Brazil and strains isolated from Central and the northern South America. The T. cruzi reference strain was not clustered with any T. rangeli strain.

Congenital heart disease and chromossomopathies detected by the karyotype

Trevisan,Patrícia; Rosa,Rafael Fabiano M.; Koshiyama,Dayane Bohn; Zen,Tatiana Diehl; Paskulin,Giorgio Adriano; Zen,Paulo Ricardo G.
Fonte: Sociedade de Pediatria de São Paulo Publicador: Sociedade de Pediatria de São Paulo
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 EN
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OBJECTIVE: To review the relationship between congenital heart defects and chromosomal abnormalities detected by the karyotype. DATA SOURCES: Scientific articles were searched in MEDLINE database, using the descriptors "karyotype" OR "chromosomal" OR "chromosome" AND "heart defects, congenital". The research was limited to articles published in English from 1980 on. DATA SYNTHESIS: Congenital heart disease is characterized by an etiologically heterogeneous and not well understood group of lesions. Several researchers have evaluated the presence of chromosomal abnormalities detected by the karyotype in patients with congenital heart disease. However, most of the articles were retrospective studies developed in Europe and only some of the studied patients had a karyotype exam. In this review, only one study was conducted in Latin America, in Brazil. It is known that chromosomal abnormalities are frequent, being present in about one in every ten patients with congenital heart disease. Among the karyotype alterations in these patients, the most important is the trisomy 21 (Down syndrome). These patients often have associated extra-cardiac malformations, with a higher risk of morbidity and mortality, which makes heart surgery even more risky. CONCLUSIONS: Despite all the progress made in recent decades in the field of cytogenetic...

Karyotype description of two Neotropical Psittacidae species: the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and the Hawk-headed Parrot, Deroptyus accipitrinus (Psittaciformes: Aves), and its significance for conservation plans

Lunardi,Vitor de Oliveira; Francisco,Mercival Roberto; Rocha,Guaracy Tadeu; Goldschmidt,Beatriz; Galetti Junior,Pedro Manoel
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2003 EN
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36.96%
Neotropical parrots are among the most threatened groups of birds in the world, and many species are facing extinction in a near future. At the same time, the taxonomic position of many species remains unclear. Karyotype analysis has been used to elucidate the phylogenetic status of many bird groups, also providing important information for both in situ and ex situ conservation plans. The objective of the present study was to describe for the first time the karyotypes of the endangered Hyacinth Macaw, Anodorhynchus hyacinthinus, and of the Hawk-headed Parrot, Deroptyus accipitrinus. A diploid number of 2n = 70 and a karyotype similar to the main pattern previously found for the genera Ara, Cyanopsitta, Aratinga, Propyrrhura, Pionites, Pionopsitta, Nandayus, and Guaruba were found for both species. These karyotype descriptions can be a starting point for the genetic monitoring of these two declining species.

Karyology of the Atlantic forest rodent Juliomys (Cricetidae): a new karyotype from southern Brazil

Paresque,Roberta; Christoff,Alexandre Uarth; Fagundes,Valéria
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2009 EN
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Juliomys is a small rodent from the family Cricetidae which inhabits the Atlantic forest and forests from Argentina to eastern Brazil. The three species recognized so far have different karyotypes. In this paper, we describe a new karyotype with 2n = 32, FN = 48 found in Juliomys specimens from a high-altitude area in the Atlantic forest of southern Brazil. The karyotype was analyzed after G- and C-banding and silver staining of the nucleolus organizer regions (Ag-NOR) and its G-banding patterns were compared with those of the newly described species Juliomys ossitenuis (2n = 20, FN = 36). The 2n = 32 karyomorph presented peculiar features and was very different from those of the other species of the genus: J. pictipes (2n = 36, FN = 34), J. rimofrons (2n = 20, FN = 34) and J. ossitenuis (2n = 20, FN = 36). Differences were mostly due to centric and tandem fusions, pericentric inversion and loss of heterochromatin. The karyotype represents a powerful tool to differentiate Juliomys species and our data suggest that the karyotype described herein belongs to a new species.

The karyotype of Adenomera diptyx (Boettger 1885) (Anura, Leptodactylidae) from northeastern Argentina

Zaracho,Víctor Hugo; Hernando,Alejandra Beatriz
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2011 EN
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In this work we analyzed the karyotype of five populations of Adenomera diptyx from Argentina after conventional staining, Ag-NOR and C-banding. All specimens presented 2n = 26 and FN = 34. The karyotype was formed by three submetacentric, one metacentric and nine telocentric pairs. Silver staining revealed that the NOR was located on a secondary constriction in pair 7. C- banding evidenced constitutive heterochromatin at the pericentromeric region of all chromosomes. The karyotype of A. diptyx was similar to that of A. hylaedactyla (2n = 26, FN = 34) and different from that of A. andreae (2n = 26, FN = 40) in the fundamental number and secondary constriction position. It also differed from the karyotypes of A. marmorata (2n = 24, FN = 34 and 36) and of A. aff. bokermanni (2n = 23, FN = 34) in diploid number. Until a comprehensive cytogenetic analysis of all the species of the genus is performed, their chromosome evolution will remain poorly understood.

Karyotype variability in species of the genus Zephyranthes Herb. (Amaryllidaceae-Hippeastreae).

FELIX, W. J. P.; FELIX, L. P.; MELO, N. F. de; OLIVEIRA, M. B. M.; DUTILH, J. H. A.; CARVALHO, R.
Fonte: Plant Systematics and Evolution, New York, v. 294, p. 263-271, 2011. Publicador: Plant Systematics and Evolution, New York, v. 294, p. 263-271, 2011.
Tipo: Artigo em periódico indexado (ALICE)
PT_BR
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Abstract In this work, the cytotaxonomic implications of the chromosomal characterization of cultivated and native Zephyranthes species described in northeastern Brazil were studied. All individuals had karyotype formed by a set of metacentric chromosomes, in addition to submetacentric and acrocentric chromosomes. In Zephyranthes robusta, 2n = 12 was observed and karyotype with formula 4M ? 2SM in somatic cells, representing the most symmetric karyotype among the investigated species. Z. sylvatica showed three different chromosome complement numbers: 2n = 12 with formula 1M ? 5SM, 2n = 12 ? 1B with1M ? 5SM ? (1B), and 2n = 18 formed by cracks. The cultivated species Z. rosea Lindl. presented 2n = 24 with 4M ? 7SM ? 1A, however Z. grandiflora Lindl. showed the same chromosome number with 2M ? 5SM ? 5A. Zephyranthes aff. rosea Lindl. presented 2n = 25 with one small metacentric forming a crack in the fourth metacentric pair. Z. brachyandra has 2n = 24 ? (1B) and formula 4M ? 3SM ? 5A ? (1B). Z. candida Herb. presented 2n = 38 and karyotype formula 9M ? 10SM. In Habranthus itaobinus numerical variation was observed, with the majority of populations showing a chromosome complement composed of 2n = 44 ? 1B with 5M ? 12SM ? 5A ? (1B), or 2n = 44 ? 3B in a single population. Mechanisms involved in the formation of these karyotypes from chromosomal imbalance data are discussed. Taken together...

Comparative karyotype analysis of populations in the Alstroemeria presliana Herbert (Alstroemeriaceae) complex in Chile

Baeza,Carlos; Finot,Víctor L.; Ruiz,Eduardo
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2015 EN
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Alstroemeria L., one of the most diverse genera of the Chilean flora and of high floricultural value, is represented by 35 species, most of them distributed between 28–38° S in the Mediterranean zone of Central Chile. There are 24 complex-forming taxa, of which 18 have conservation problems (8 are considered “endangered” and 10 as “vulnerable”). One of these complexes is Alstroemeria presliana Herb. with two subspecies: subsp. presliana and subsp. australis Bayer. Alstroemeria presliana grows in Chile and Argentina: subsp. presliana is distributed from Reserva Nacional Siete Tazas (35°27′ S, Region of Maule) to Antuco, (37°25′ S, Region of Bío-Bío), and is also found in Neuquén, Argentina; subsp. australis is endemic to the Cordillera of Nahuelbuta. A comparative karyotype study was carried out among six populations of A. presliana subsp. presliana and five populations of A. presliana subsp. australis. The eleven populations presented an asymmetric karyotype, with 2n = 2× = 16 chromosomes but with different karyotype formulae. A. presliana subsp. presliana shows the haploid formula 2m + 2m-sat + 1sm-sat + 1st-sat + 1t + 1 t-sat, and A. presliana subsp. australis presents a formula 1m + 2m-sat + 1sm + 2t + 2t-sat chromosomes. The architecture of the karyotype between the subspecies is very different. The scatter plot among CVCL vs. MCA shows different groupings between populations of the two subspecies. According to the results obtained it is possible to consider raising Alstroemeria presliana subsp. australis at species level.

Chromosomal size conservation through the cell cycle supports karyotype stability in Trypanosoma cruzi

Toro, Gabriela Cecilia; Ruiz, Andres Mariano; Sabaj, Valeria; López Solís, Remigio; Espinoza, Ingrid; Galanti, Norbel; Búa, Jaqueline; Grenet, Jose; Galindo, Mario
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artículo de revista
EN
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The Trypanosoma cruzi karyotype shows an extensive chromosomal size polymorphism. Absence of condensed mitotic chromosomes and chromatin fragility are characteristic features of T. cruzi which would allow DNA breaks and chromosomal rearrangements during cell proliferation. We have investigated by pulsed field gel electrophoresis (PFGE) eventual changes in chromosomal size during exponential and stationary phases of T. cruzi epimastigotes in culture, in G0 trypomastigotes and throughout the cell cycle in synchronized epimastigotes. T. cruzi molecular karyotype was stable throughout the cell cycle and during differentiation. Thus, the chromosomal size polymorphism previously reported in T. cruzi contrasts with the stability of the molecular karyotype observed here and suggests that chromosomal rearrangements leading to changes in chromosomal size are scarce events during the clonal propagation of this parasite; Supported by a Grant from the Programa Bicentenario de Ciencia y Tecnologı´a, Proyecto Anillo ACT29, The World Bank/CONICYT, Chile.

Karyotype characterization of Argania spinosa (L.) Skeel (Sapotaceae)

Majourhat, Khalid; Jabbar, Y.; Araneda, L.; Zeinalabedini, Mehrshad; Hafidi, Abdellatif; Martínez-Gómez, Pedro
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
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3 pages, 1 figure.; Argania spinosa (L.) Skeel is an endemic species from Southwest Morocco being the unique representative of the tropical Sapotaceae in this area. The cytology of this species is poorly known in spite of its great socio-economical and ecological interest in these arid and semi-arid zones. The objective of this work is to characterize the karyotype of A. spinosa species in somatic cells from root tips. Samples analyzed showed a karyotype constituted for ten pairs of chromosomes (2n = 2x = 20) and the putative karyotype proposed has been of four submetacentric and six metacentric pairs. The four submetacentric pairs were the longest with a mean total length between 1.14 and 1.69 μm and the total length of six metacentric pairs were between 0.59 and 1.03 μm.; This work has been supported by grant A/6210/06 “Caractérisation biochimique et génomiques des morphotypes de l´arganier (Argania spinosa L. Skeels)” from the AECI (Agencia Española de Cooperación Internacional) of Spain.; Peer reviewed

Contribuição ao estudo do cariótipo do carneiro doméstico Ovis aries; Determination of the Karyotype of sheep (Ovis aries, L.) raised in Brazil

Oliveira Filho, Enoch Borges de
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; Formato: application/pdf
Publicado em 15/09/1978 POR
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This study was conducted aiming to give elements for determination of the karyotype of sheep (Ovis aries, L) raised in Brazil. Six males, not cast rated, Ideal crossbred, at eight to nine months of age, were used, belonging to the College of Medicine of Ribeirão Preto, SP. The lynphocytes culture followed the MORHEAD and cols, technics, modified by FERRARI. Good quality metaphases were photomicrographed for Karyotype mounting. The SCHERES technics, modified, was used for G bands . The diploid number of 54 chromosome was confirmed. There were no difficulties, for Karyotype mounting, toward the six large metacentric autosomes and sexual chromosomes, browever for the 46 acrocentric antosomes , only with G banding should be attained a certain degree of confidence the determination of a banding pattern was not obtained, being suggested new investigations on this matter.; Visou-se fornecer elementos para a determinação do cariótipo de carneiros (Ovis aires L.) criados no Brasil. Foram utilizados seis machos, não castrados, mestiços da raça Ideal, com oito a nove meses de idade, do Biotério da Faculdade de Medicina de Ribeirão Preto. SP. A cultura de linfócitos seguiu a técnica de MOORHEAD e Cols. modificada por FERRARI. As metáfases de boa qualidade eram fotomicrografadas para a montagem do cariótipo. Foi utilizada a técnica de SCHERES modificada para bandas G. Foi confirmado o número displóide de 54 cromossomos. Para a montagem do cariótipo...