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"Avaliação da expressão dos receptores de interleucina-8, CXCR1 e CXCR2, e da atividade proliferativa em fibroblastos de quelóide e de pele normal" ; Determination of the interleukin-8 receptors CXCR1 and CXCR2, and proliferative activity in keloids and normal skin fibroblasts

Abdo Filho, Décio
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 05/09/2006 PT
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O quelóide é um tumor fibroso benigno que ocorre durante a cicatrização da pele em indivíduos geneticamente predispostos. A cicatrização é um processo biológico complexo e depende da interação de diferentes estruturas teciduais e de um grande número de tipos celulares residentes e infiltrativos, que produzem citocinas. A interleucina 8 (IL-8), citocina pró-inflamatória, é super-expressa pelos fibroblastos durante o desenvolvimento do tecido de granulação, acelerando o processo de cicatrização. Como o quelóide resulta de uma reparação tecidual anormal após lesão da pele, o presente estudo teve por objetivo determinar a expressão dos receptores da IL-8, CXCR1 e CXCR2, e a capacidade proliferativa, pelo ciclo celular, dos fibroblastos queloideanos cultivados e extraídos ex vivo, por citometria de fluxo. Fibroblastos de cicatriz queloideana e de pele normal foram obtidos de 21 pacientes da raça negra, com idade variando entre 10 e 40 anos, de lesões com até 2 anos de evolução. Em nosso estudo constatamos expressão reduzida dos receptores para a IL-8, CXCR1(35,7%±11,2) e CXCR2 (27,8%±11,3), em fibroblastos de cicatriz queloideana cultivados, comparando com a pele normal (44,1±16,2 e 46,3±27,1 respectivamente). Entretanto...

Efeito da metformina sobre IL-8 e IL-1b em um modelo de células estromais endometriais hiperinsulinêmicas e hiperandrogênicas in vitro

Machado, Amanda de Barros
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
POR
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O endométrio é a mucosa que reveste o útero. A receptividade uterina é definida como um estado em que o endométrio se encontra receptivo à implantação do blastocisto. E, a preparação do endométrio para a implantação não é somente uma questão de estimulação hormonal adequada, depende da interação entre o blastocisto e o endométrio. Esta interação envolve uma complexa sequência de eventos de sinalização e uma variedade de moléculas. As concentrações de interleucina-8 (IL-8) e interleucina-1β (IL-1β) estão correlacionadas com o processo de implantação. Em humanos, a taxa de insucesso desse processo é alta e ocasionada por diversos fatores. A síndrome dos ovários policísticos (SOP) é um distúrbio endócrino-ginecológico que afeta de 6 a 8 % das mulheres em idade reprodutiva, e se caracteriza, principalmente, por anovulação crônica e hiperandrogenismo, estando diretamente relacionada à infertilidade feminina. Apesar da incerteza sobre a causa primária da SOP, há relatos sobre a importância da hiperinsulinemia na sua promoção. O objetivo deste trabalho foi estabelecer um modelo de hiperinsulinemia e hiperandrogenismo em células estromais endometriais in vitro, simulando características de SOP; identificar o melhor gene normalizador para estudos de expressão gênica em amostras das células em cultivo; avaliar o efeito da metformina sobre a proliferação celular e expressão gênica da IL-8 e IL-1β no modelo proposto. O tecido endometrial foi obtido de pacientes submetidas a histerectomia. A cultura primária das células estromais foi padronizada e as células foram divididas em sete grupos de tratamento: estradiol (G1); estradiol e progesterona (G2); estradiol...

Haplotypes in the Interleukin 8 Gene and Their Association with Chronic Periodontitis Susceptibility

Scarel-Caminaga, Raquel M.; Kim, Yeon J.; Viana, Aline C.; Curtis, Karen M. C.; Corbi, Samia C. T.; Sogumo, Patricia M.; Orrico, Silvana R. P.; Cirelli, Joni A.
Fonte: Springer/plenum Publishers Publicador: Springer/plenum Publishers
Tipo: Artigo de Revista Científica Formato: 292-302
ENG
Relevância na Pesquisa
65.98%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 03/10424-0; Processo FAPESP: 05/03231-7; Processo FAPESP: 06/04492; Interleukin-8 (IL-8), which is responsible for the migration and activation of neutrophils, is an important inflammatory mediator involved in the initiation and amplification of acute inflammatory reactions and chronic inflammatory processes. IL-8 plays an important role in periodontitis, an inflammatory disease characterized by the loss of connective tissue and alveolar bone. The aim of this study was to investigate whether the SNPs rs2227307 (+396) and rs2227306 (+781), and the haplotypes they formed together with the previously investigated rs4073 (-251), were associated with chronic periodontitis susceptibility. Clinical periodontal exams were performed and DNA samples were collected from 493 individuals (223 with periodontitis and 270 controls). Associations between SNPs, haplotypes, and subject phenotypes were analyzed using the chi(2) test followed by multivariate logistic regression modeling. We conclude that the +396TT genotype and the haplotypes ATC/TTC and AGT/TGC were significantly associated with chronic periodontitis susceptibility in Brazilians.

Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay

Kim, Yeon Jung; Viana, Aline Cavalcanti; Tfaile Corbi, Samia Cruz; de Carvalho Curtis, Karen Maria; Renzi, Rivelto; Perez Orrico, Silvana Regina; Cirelli, Joni Augusto; Scarel-Caminaga, Raquel Mantuaneli
Fonte: Universidade Federal de Uberlândia (UFU) Publicador: Universidade Federal de Uberlândia (UFU)
Tipo: Artigo de Revista Científica Formato: 136-142
ENG
Relevância na Pesquisa
65.98%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Processo FAPESP: 03/10424-0; Processo FAPESP: 05/03231-7; Processo FAPESP: 05/04553-8; Processo FAPESP: 06/04492-1; Interleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient...

Interleukin-8 as a prognostic serum marker in canine mammary gland neoplasias

Gelaleti, Gabriela Bottaro; Jardim, Bruna Victorasso; Leonel, Camila; Moschetta, Marina Gobbe; Pires de Campos Zuccari, Debora Ap
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 106-112
ENG
Relevância na Pesquisa
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Mammary gland tumors in female dogs are an excellent model for the clinic-pathological, diagnostic and prognostic investigation of mammary neoplasias. Prognostic and predictive markers are effective in research and routine diagnosis. Interleukins play a fundamental role in cancer, with a particular function in tumor growth, invasion and metastatic potential. Interleukin-8 (IL-8) is known to possess tumorigenic and pro-angiogenic properties, and its overexpression is seen in a number of human tumors. IL-8 serum levels were determined and correlated with the clinic-pathological features and clinical evolution of mammary gland neoplasias in female dogs. IL-8 was measured by an immunoenzymatic assay in 30 female dogs with mammary neoplasias within a 12 month follow-up and in 50 control animals. The correlation between IL-8 concentration and clinical parameters was investigated. A statistically significant difference in the IL-8 serum levels was found in tumor-bearing dogs compared to the controls. In addition, when the individual parameters were evaluated, IL-8 content showed a positive correlation with the tumor progression, lymph node involvement, recurrence and death. Single and multivariate analyses showed associations between tumor recurrence...

An immunohistochemical study of interleukin-8 (IL-8) in breast cancer

Pires de Campos Zuccari, Debora Aparecida; Leonel, Camila; Castro, Rodrigo; Gelaleti, Gabriela Bottaro; Jardim, Bruna Victorasso; Moscheta, Marina Gobbe; Regiani, Vitor Rafael; Ferreira, Livia Carvalho; Lopes, Juliana Ramos; Neto, Dalisio de Santi; Esteve
Fonte: Elsevier Gmbh, Urban & Fischer Verlag Publicador: Elsevier Gmbh, Urban & Fischer Verlag
Tipo: Artigo de Revista Científica Formato: 571-576
ENG
Relevância na Pesquisa
66.15%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); The use of prognostic markers for breast cancer is important for routine diagnosis and research. Interleukin-8 is a chemotactic cytokine produced by several cell types in response to inflammation, however, its expression, regulation and function are poorly understood. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. The present study investigated the correlation between interleukin-8 expression and breast cancer prognosis. Interleukin-8 expression was assessed in 72 women with mammary neoplasia by immunohistochemistry and the results were statistically correlated with clinical-pathological findings. There was an inverse correlation between interleukin-8 expression and metastasis (p = 0.03) and/or local recurrence (p = 0.02). In the patient group that received post-surgery chemotherapy and radiotherapy, a lower interleukin-8 expression was found in those women that showed local recurrence (p = 0.01). Multivariate logistic regression showed estrogen receptor negativity, progesterone positivity and metastasis with increased risk of death (p < 0.05). The data reflect the complexity of the role of interleukin-8 in tumor microenvironment and support its classification as a possible prognostic marker...

Interleukin-8 expression associated with canine mammary tumors

Zuccari, D. A. P. C.; Castro, R.; Gelaleti, G. B.; Mancini, U. M.
Fonte: Funpec-editora Publicador: Funpec-editora
Tipo: Artigo de Revista Científica Formato: 1522-1532
ENG
Relevância na Pesquisa
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 05/51936-0; The use of prognostic markers for mammary cancer is important for routine diagnosis and research. Interleukin-8 (IL-8) is a chemotactic cytokine, produced by several cell types in response to inflammation. The expression, regulation and function of IL-8 in dogs are little known. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. We investigated a possible correlation between IL-8 expression and mammary tumor prognosis in female dogs. IL-8 expression was measured in 50 dogs with mammary neoplasia by immunohistochemistry and real-time PCR. Immunohistochemical staining was done with anti-IL-8 antibodies and PCR amplifications were performed in a 7500 Fast Real-Time PCR system. Gene expression stability was analyzed by the geNorm software. Quantitative real-time PCR showed that IL-8 expression decreased in malignant mammary cells compared to normal mammary tissue, while weak immunostaining was associated with a diagnosis of carcinoma. Complementing earlier studies on IL-8 expression in several types of cancer, including mammary cancer, we conclude that IL-8 has potential for use as a prognostic marker for canine mammary neoplasia.

Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer

Vinagre,Ruth Maria Dias Ferreira; Corvelo,Tereza Cristina de Oliveira; Arnaud,Vanda Catão; Leite,Ana Claudia Klautau; Barile,Katarine Antonia dos Santos; Martins,Luisa Caricio
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2011 EN
Relevância na Pesquisa
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CONTEXT: Gastric neoplasia is the second most common cause of death by cancer in the world and H. pylori is classified as a type I human carcinogen by the World Health Organization. However, despite the high prevalence of infection by H. pylori around the world, less than 3% of individuals carrying the bacteria develop gastric neoplasias. Such a fact indicates that evolution towards malignancy may be associated with bacterial factors in the host and the environment. OBJECTIVES: To investigate the association between polymorphism in the region promoting the IL-8 (-251) gene and the H. pylori genotype, based on the vacA alleles and the presence of the cagA gene, using clinical and histopathological data. METHODS: In a prospective study, a total of 102 patients with stomach cancer and 103 healthy volunteers were analysed. Polymorphism in interleukin 8 (-251) was determined by the PCR-restriction fragment length polymorphism reaction and sequencing. PCR was used for genotyping the vacA alleles and the cagA in the bacterial strains PCR. Gastric biopsies were histologically assessed. RESULTS: The H. pylori serology was positive for 101 (99%) of all patients analysed, and 98 (97%) of them were colonized by only one strain. In patients with monoinfection...

Abnormal spontaneous interleukin 8 receptor expression: a brief report of two cases

Antas,Paulo; Holland,Steven; Sterling,Timothy
Fonte: Sociedade Brasileira de Medicina Tropical - SBMT Publicador: Sociedade Brasileira de Medicina Tropical - SBMT
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2012 EN
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Interleukin 8 (CXCL8) is an autocrine chemokine specific for the chemoattraction and activation of granulocytes, NKT cells and T lymphocytes. Patients with tuberculosis and latent Mycobacterium tuberculosis infection were assessed for the spontaneous expression of CXCR1 (CD128) and CXCR2 on lymphocytes and monocytes. Compared with ex vivo profiles, increased spontaneous CXCR2 expression and normal CXCR1 expression were found on lymphocytes in two out of 59 individuals. Monocytes showed normal ex vivo profiles for both receptors. After stimulation with purified protein derivative, the in vitro levels of CXCL8 were below the median levels of all patients with prior tuberculosis. Spontaneous CXCR2 modulation did not cause notable variation in the in vitro levels of CXCL8.

Participation of interleukin-5, interleukin-8 and leukotriene B4 in eosinophil accumulation in two different experimental models

Oliveira,Sandra HP; Faccioli,Lúcia H; Ferreira,Sérgio H; Cunha,Fernando Q
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/1997 EN
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There are several experimental models describing in vivo eosinophil (EO) migration, including ip injection of a large volume of saline (SAL) or Sephadex beads (SEP). The aim of this study was to investigate the mechanisms involved in the EO migration in these two models. Two consecutive injections of SAL given 48 hr apart, induced a selective recruitment of EO into peritoneal cavity of rats, which peaked 48 hr after the last injection. SEP, when injected ip, promoted EO accumulation in rats. The phenomenom was dose-related and peaked 48 hr after SEP injection. To investigate the mediators involved in this process we showed that BW A4C, MK 886 and dexamethasone (DXA) inhibited the EO migration induced by SAL and SEP. To investigate the source of the EO chemotactic factor we showed that mast cells, macrophages (MO), but not lymphocytes, incubated in vitro in presence of SAL released a factor which induced EO migration. With SEP, only mast cells release a factor that induced EO migration, which was inhibited by BW A4C, MK 886 and DXA. Furthermore, the chemotactic activity of SAL-stimulated mast cells was inhibited by antisera against IL-5 and IL-8 (interleukin). SAL-stimulated MO were only inhibited by anti-IL-8 antibodies as well SEP-stimulated mast cells. These results suggest that the EO migration induced by SAL may be dependent on resident mast cells and MO and mediated by LTB4...

Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer

VINAGRE, Ruth Maria Dias Ferreira; CORVELO, Tereza Cristina de Oliveira; ARNAUD, Vanda Cat?o; LEITE, Ana Claudia Klautau; BARILE, Katarine Antonia dos Santos; MARTINS, Luisa Caricio
Fonte: Universidade Federal do Pará Publicador: Universidade Federal do Pará
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
56.11%
CONTEXTO: A neoplasia g?strica ? a segunda causa mais comum de morte por c?ncer no mundo e o H. pylori ? classificado como carcin?geno humano tipo I pela Organiza??o Mundial de Sa?de. Entretanto, apesar da elevada preval?ncia da infec??o pelo H. pylori em todo mundo, menos de 3% de indiv?duos portadores dessa bact?ria desenvolvem neoplasias g?stricas. Tal fato indica que a evolu??o para maligniza??o possa estar associada a fatores bacterianos, do hospedeiro e do ambiente. OBJETIVOS: Investigou-se a associa??o do polimorfismo da regi?o promotora do gene IL-8 (-251) e do gen?tipo do H. pylori, baseado nos alelos vacA e na presen?a do gene cagA, com a cl?nica e os dados histopatol?gicos. M?TODOS: Em estudo prospectivo, 102 pacientes com c?ncer g?strico e 103 volunt?rios saud?veis foram analisados. O polimorfismo da IL-8 (-251) foi determinado pela rea??o de PCR-RFLP e sequenciamento. Para genotipagem dos alelos vacA e do gene cagA das cepas bacterianas foi utilizada a PCR. As biopsias g?stricas foram avaliadas histologicamente. RESULTADOS: A sorologia para o H. pylori foi positiva em 101 (99%) de todos os pacientes analisados, e 98 (97%) deles foram colonizados por apenas uma cepa bacteriana. Em pacientes com monoinfec??o, 82 (84%) das cepas bacterianas observadas apresentavam o gen?tipo s1b/m1. O gene cagA foi detectado em 74 (73%) dos pacientes infectados pelo H. pylori. A presen?a do gene cagA demonstrou estar associada com a presen?a do gen?tipo s1b/m1 do gene vacA (P = 0...

Diverging signal transduction pathways activated by interleukin 8 and related chemokines in human neutrophils II: IL-8 and Groa differentially stimulate calium influx through IL-8 receptors A & B

Damaj, B.; McColl, S.; Neote, K.; Hebert, C.; Naccache, P.
Fonte: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC Publicador: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Tipo: Artigo de Revista Científica
Publicado em //1996 EN
Relevância na Pesquisa
66.11%
Interleukin 8 (IL-8) and Gro-α are members of the CXC branch of a family of cytokines recently designated the “chemokine” superfamily. Recent evidence indicates that, contrary to previously held beliefs, IL-8 and Gro-α may not be perceived equivalently by neutrophils. In this study, we have evaluated the effects of IL-8 and Gro-α on the rate of calcium influx in human neutrophils and in 293 cells transfected with type A or type B IL-8 receptors. Of these two chemokines, only Gro-α induced an influx of calcium in neutrophils as judged by the sensitivity of the mobilization of calcium to the extracellular calcium chelator EGTA and to the nonselective divalent cation channel inhibitor SK&F 96365, as well as by manganese quenching experiments. IL-8 was similarly without effect on the rate of Mn²⁺ influx in 293 cells transfected with IL-8 receptor A (IL-8RA) or IL-8RB. On the other hand, Gro-α induced an SK&F 96365-sensitive increase of the rate of Mn⁺² influx in IL-8RB-, but not in IL-8RA-transfected 293 cells. These results indicate not only that neutrophils respond differently to IL-8 than they do to Gro-α but, furthermore, that the consequences of the binding of IL-8 and Gro-α to IL-8RB are distinct.; Bassam B. Damaj...

Physical association of Gi₂α with Interleukin-8 receptors; Physical association of Gi(2)alpha with Interleukin-8 receptors

Damaj, B.; McColl, S.; Mahana, W.; Crouch, M.; Naccache, P.
Fonte: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC Publicador: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Tipo: Artigo de Revista Científica
Publicado em //1996 EN
Relevância na Pesquisa
66.13%
Interleukin-8 (IL-8), one of the major mediators of the inflammatory response, belongs to a family of chemokines that includes NAP-2 (Graphiceutrophil-Graphicctivating Graphiceptide-2) and Gro-α and whose biological activities are directed to a great extent toward neutrophils. Two distinct receptors have been described with overlapping, but not identical, binding affinities for IL-8, NAP-2, and Gro-α. This study was designed to examine the intracellular pathways activated upon the occupation of each of the IL-8 receptors (IL-8R). The formation of a physical coupling between IL-8 receptors and the α-subunit of heterotrimeric G proteins was tested in neutrophils by examining the presence of the former in anti-Gα immune precipitates. The addition of IL-8 to a suspension of human neutrophils led to a time-dependent detection of IL-8 in anti-Gi2α (raised against amino acids 159-168 (LERIAQSDYI) of Gi2α) and anti-Gtα (raised against the COOH-terminal 10 amino acids (KENLKDCGLF) of Gtα), but not anti-Gq, immunoprecipitates. Similar results were obtained in human 293 cells stably transfected with IL-8RA or IL-8RB. The peptide derived from the COOH-terminal sequence of Gt inhibited the co-immunoprecipitation of IL-8R and Gi observed in response to the anti-Gtα and anti-Gi2α antibodies. On the other hand...

Shiga toxin 2 and Flagellin from Shiga-toxigenic Escherichia coli superinduce interleukin-8 through synergistic effects on host stress-activated protein kinase activation

Jandhyala, D.; Rogers, T.; Kane, A.; Paton, A.; Paton, J.; Thorpe, C.
Fonte: Amer Soc Microbiology Publicador: Amer Soc Microbiology
Tipo: Artigo de Revista Científica
Publicado em //2010 EN
Relevância na Pesquisa
66.17%
Shiga toxins expressed in the intestinal lumen during infection with Shiga-toxigenic Escherichia coli must translocate across the epithelium and enter the systemic circulation to cause systemic (pathological) effects, including hemolytic uremic syndrome. The transepithelial migration of polymorphonuclear leukocytes in response to chemokine expression by intestinal epithelial cells is thought to promote uptake of Stx from the intestinal lumen by compromising the epithelial barrier. In the present study, we investigated the hypothesis that flagellin acts in conjunction with Shiga toxin to augment this chemokine expression. We investigated the relative contributions of nuclear factor B (NF-B) and mitogen-activated protein kinase (MAPK) signaling to transcription and translation of interleukin-8. Using reporter gene constructs, we showed that flagellin-mediated interleukin-8 gene transcription is heavily dependent on both NF-B and extracellular signal-regulated kinase 1 and 2 (ERK-1/2) activation. In contrast, inhibition of p38 has no detectable effect on interleukin-8 gene transcription, even though flagellin-mediated activation of host p38 is critical for maximal interleukin-8 protein expression. Inhibition of MAPK-interacting kinase 1 suggests that p38 signaling affects the posttranscriptional regulation of interleukin-8 protein expression induced by flagellin. Cotreatment with Stx2 and flagellin results in a synergistic upregulation of c-Jun N-terminal protein kinases (JNKs)...

Interleukin-8-Konzentration in Plasma und in lysiertem Vollblut : Referenzwerte bei gesunden Neugeborenen und die Wertigkeit bei neonataler bakterieller Infektion; interleukin-8 concentrations in plasma and lysed whole blood in newborns and those with early-onset bacterial infection

Neunhoeffer, Felix Sebastian Daniel
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
DE_DE
Relevância na Pesquisa
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Nach wie vor zählt die neonatale bakterielle Infektion (NBI) aufgrund der hohen Morbidität und Mortalität zu den Hauptgefahren für das neugeborene Kind. IL-8 in Plasma ist ein bedeutender Parameter für die Diagnostik einer neonatalen bakteriellen Infektion, besitzt aber nur eine kurze Halbwertszeit. IL-8, in Vollblut-Lysat bestimmt, setzt sich sowohl aus freiem extrazellulären als auch aus zellgebundenen IL-8 zusammen. Ziel dieser Arbeit war, die Kinetik von IL-8 in Plasma und in Lysat bei gesunden Neugeborenen und bei Neugeborenen mit Verdacht auf NBI zu untersuchen und zu testen, ob die Bestimmung von IL-8 in Lysat eine höhere Sensitivität für die Diagnostik der NBI ermöglicht. 61 Neugeborene mit NBI konnten untersucht werden; die Kontrollgruppe bestand aus 188 Neugeborenen, die Risikofaktoren für NBI zeigten, aber nicht erkrankten. In der Kontrollgruppe fanden sich bis zu 24 Stunden nach Geburt 280-fach höhere IL-8 Konzentrationen in Lysat (9599 pg/ml; SD 4433) als in Plasma (34,2 pg/ml; 18,1). Die Sensitivität von IL-8 in Lysat versus IL-8 in Plasma für NBI betrug innerhalb der ersten 6 Lebensstunden 97% versus 71% und nach 24 Stunden 70% versus 32%. Die zugehörige Spezifität lag innerhalb der ersten 6 Lebensstunden bei 95% versus 90% und nach 24 Stunden bei 92% versus 99%. Der negativ prädiktive Wert für IL-8 in Lysat versus IL-8 in Plasma war 80% versus 66%. IL-8 in Lysat zeigte im Vergleich zu IL-8 in Plasma zum Zeitpunkt des Verdachts auf NBI eine höhere Sensitivität und blieb länger erhöht. Bei gesunden Reifgeborenen waren die IL-8 Werte in Plasma und Lysat vom Geburtsmodus abhängig. Anhand der Kinetik bei unterschiedlichen Geburtsmodi und bei Neugeborenen mit NBI schien das „IL-8-Gedächtnis“ des Pools zirka 24 Stunden zu reichen. Die IL-8-Bestimmung in Lysat bedeutet somit einen entscheidenden Vorteil im kritischen Zeitfenster einer beginnenden NBI.; Background and objective: In neonatal bacterial infection (NBI)...

Modulation proinflammatorischer Mechanismen durch physiologische Darmbakterien am Beispiel der durch Yersinia enterocolitica pYV- induzierten Interleukin-8-Sekretion; Modulation of proinflammatory mechanisms by physiologic intestinal bacteria using the example of Yersinia enterocolitica pYV- induced Interleukin-8 secretion

Quitadamo, Matteo
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
DE_DE
Relevância na Pesquisa
66.14%
Die Bakterien der physiologischen Darmflora spielen eine wichtige Rolle in der Homöostase des Intestinaltraktes, u.a durch Stimulation und Modulation des Immunsystems. Von besonderem Interesse sind hierbei sogenannte probiotische Bakterien, also Mikroorganismen, die per Definition für den Wirt vorteilhafte Wirkungen entfalten. Ziel der vorliegenden Arbeit war es, die Modulation proinflammatorischer Mechanismen durch physiologische Darmbakterien zu untersuchen, um neue Einblicke in die Wirkungsmechanismen probiotischer Mikroorgansimen zu bekommen. Als Modell einer proinflammatorischen Reaktion wurde die über Invasin-/Integrin-Signaling vermittelte Interleukin-8-Induktion in HeLa-Zellen durch den enteropathogenen Stamm Yersinia enterocolitica pYV- gewählt, da die hieran beteiligten Mechanismen auf molekularer Ebene bereits gut charakterisiert sind. Untersucht wurden verschiedene kommensale Bakterienstämme, darunter der bekanntermaßen probiotische Stamm E. coli Nissle 1917, verschiedene apathogene E. coli-Stämme sowie eine Reihe weiterer Stämme, die aus den Fäzes gesunder Erwachsener isoliert wurden und zu den Gattungen Lactobacillus, Bifidobacterium, Streptococcus, Bacteroides sowie Veillonella gehören. Nach Charakterisierung der Testbakterien im Hinblick auf Adhäsion...

Einflussfaktoren auf Interleukin-8-Konzentrationen in Plasma und lysiertem Vollblut beim Neugeborenen; Influencing factors on plasma and DLWB interleukin-8-concentrations in newborns

Siedler, Diana
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
DE_DE
Relevância na Pesquisa
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Einflussfaktoren auf Interleukin-8-Konzentrationen in Plasma und lysiertem Vollblut beim Neugeborenen D. Siedler, M. Deperschmidt#, C. Henkel#, M. Eichner*, Ch. F. Poets, Th. Orlikowsky Univ.-Kinderklinik Tübingen, Abt. Neonatologie, #Univ.-Frauenklinik Tübingen, *Institut für medizinische Biometrie Tübingen Hintergrund: Trotz wirksamer Antibiotikatherapie ist die frühe neonatale bakterielle Infektion (EOBI) mit einer hohen Morbidität und Mortalität verbunden. Deshalb ist die frühzeitige Diagnose und Behandlung wichtig, um Spätfolgen zu verhindern und eine normale Entwicklung des Kindes zu gewährleisten. IL-8 ist ein Akutphase-Zytokin, welches früh bei bakteriellen Infektionen ausgeschüttet wird. Es wird neben den spezifischen IL-8-Rezeptoren auf Granulozyten auch unspezifisch an Erythrozyten gebunden. Im Plasma gemessen ist seine Halbwertszeit kurz und beträgt ein bis drei Stunden. In lysiertem Vollblut erwies es sich als Parameter, welcher der Plasmabestimmung im Hinblick auf Sensivität, Spezifität sowie dem prädiktiven Wert bei EOBI überlegen war. Ziel: Untersuchung von Einflussgrößen prä-, peri- und postnataler Faktoren, hierbei insbesondere des Erythrozytenstatus, auf IL-8-Konzentrationen in Plasma und lysiertem Vollblut...

Serielle Lactatbestimmung als prognostischer Parameter nach Herzoperationen im Kindesalter - Vergleich mit der prognostischen Aussagekraft von Procalcitonin, Interleukin 6 und 8 in Bezug auf das postoperative Geschehen nach Herzoperationen im Kindesalter; Serial measurement of lactate as a prognostic parameter after heart surgery in children- a comparison with the prognostic meaning of procalcitonin, interleukin 6 and 8 referring to the postoperative course in infancy

Weiß, Christine
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
DE_DE
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Lactat entsteht bei der anaeroben Glykolyse. Das postoperative Lactat ermöglicht, das Ausmaß der intraoperativen Gewebehypoxie mit eventuellen Folgeschäden abzuschätzen. Procalcitonin, ein aus 116 Aminosäuren bestehendes Protein, und die Interleukine 6 und 8, zur Gruppe der Zytokine gehörend, dienen als Inflammationsmarker. Die vorliegende Arbeit besteht aus zwei Teilen. Im ersten Teil wurde im Rahmen einer retrospektiven Studie bei 253 an der Universitäts-Kinderklinik Tübingen in der Zeit von März 1997 bis Mai 1998 am Herzen operierten Kinder die postoperativen Lactatspiegel bestimmt. Die Kinder waren zwischen einem Tag und 16,5 Jahren alt. Ein Ziel war es herauszufinden, ob das Lactat ein Parameter ist, der zur Vorhersage des postoperativen Outcomes beitragen kann. Im zweiten Teil, einer prospektiven Studie, wurden Lactatspiegel bei 51 Kindern, die in der Zeit von August 2000 bis Februar 2001 ebenfalls an der herzchirurgischen Abteilung der Universitätskinderklinik Tübingen am Herzen operiert wurden, untersucht. Diese Kinder waren zwischen 4 Tagen und 16 Jahre alt. Die Lactatverläufe wurden bis zum 6. postoperativen Tag analysiert. Zusätzlich wurde sieben Mal bis zum 6. postoperativen Tag die Bestimmung von Procalcitonin (PCT)...

Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease

Vendramini,Alex Augusto; Lábio,Roger Willian de; Rasmussen,Lucas Trevizani; Reis,Nathali Mattiuzo dos; Minett,Thais; Bertolucci,Paulo Henrique Ferreira; Pinhel,Marcela Augusta de Souza; Souza,Dorotéia Rossi Silva; Mazzotti,Diego Robles; Smith,Marília d
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2011 EN
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56.09%
An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin-1α (IL-1α), have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8 -251T > A (rs4073) and IL-1α-889C > T (rs1800587) and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young controls were obtained. DNA samples were isolated from blood cells, and the PCR-RFLP method was used for genotyping. The genotype distributions of polymorphisms IL-8, IL-1α and APOE were as expected under Hardy-Weinberg equilibrium. The allele frequencies did not differ significantly among the three groups tested. As expected, the APOE4 allele was strongly associated with AD (p < 0.001). No association of AD with either the IL-1α or the IL-8 polymorphism was observed...

Interleukin 8 as a vaso-occlusive marker in Brazilian patients with sickle cell disease

Gonçalves,M.S.; Queiroz,I.L.; Cardoso,S.A.; Zanetti,A.; Strapazoni,A.C.; Adorno,E.; Albuquerque,A.; Sant'Ana,A.; Reis,M.G. dos; Barral,A.; Barral Netto,M.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2001 EN
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66.03%
Sickle cell disease has a worldwide distribution and is a public health problem in Brazil. Although vaso-occlusive crisis (VOC) is one of the most important clinical features of the disease, there are still several steps of its pathogenesis which are unknown. The increase of the chemotactic factor interleukin 8 (IL-8) has been reported to be involved in sickle cell disease crisis, but this has not been demonstrated conclusively. In the present study we analyzed serum IL-8 levels by ELISA and hematological parameters and hemoglobin patterns by standard techniques in 23 (21 SS and 2 SC) Brazilian patients with sickle cell syndromes during VOC caused by different inducing factors, 22 (21 SS and 1 SC) sickle cell patients out of crisis, and 11 healthy controls. Increased IL-8 levels were observed in 19 of 23 VOC patients (79.2%), 3 of them with more than 1,000 pg/ml. Seventeen of 22 (77.3%) non-crisis patients showed low IL-8 levels (less than 15 pg/ml). Healthy controls had low IL-8 levels. A significant difference in serum IL-8 levels was observed between crisis and non-crisis sickle cell patients (P<0.0001). There was no correlation between IL-8 levels and hematological data or hemoglobin patterns. High serum IL-8 levels were observed in VOC patients independently of the crisis-inducing factor. We conclude that in the studied population...