Página 1 dos resultados de 14443 itens digitais encontrados em 0.018 segundos

Immunohistochemical analysis of neuron types in the mouse small intestine

QU, Zheng-Dong; THACKER, Michelle; CASTELUCCI, Patricia; BAGYANSZKI, Maria; EPSTEIN, Miles L.; FURNESS, John B.
Fonte: SPRINGER Publicador: SPRINGER
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
36.6%
The definition of the nerve cell types of the myenteric plexus of the mouse small intestine has become important, as more researchers turn to the use of mice with genetic mutations to analyze roles of specific genes and their products in enteric nervous system function and to investigate animal models of disease. We have used a suite of antibodies to define neurons by their shapes, sizes, and neurochemistry in the myenteric plexus. Anti-Hu antibodies were used to reveal all nerve cells, and the major subpopulations were defined in relation to the Hu-positive neurons. Morphological Type II neurons, revealed by anti-neurofilament and anti-calcitonin gene-related peptide antibodies, represented 26% of neurons. The axons of the Type II neurons projected through the circular muscle and submucosa to the mucosa. The cell bodies were immunoreactive for choline acetyltransferase (ChAT), and their terminals were immunoreactive for vesicular acetylcholine transporter (VAChT). Nitric oxide synthase (NOS) occurred in 29% of nerve cells. Most were also immunoreactive for vasoactive intestinal peptide, but they were not tachykinin (TK)-immunoreactive, and only 10% were ChAT-immunoreactive. Numerous NOS terminals occurred in the circular muscle. We deduced that 90% of NOS neurons were inhibitory motor neurons to the muscle (26% of all neurons) and 10% (3% of all neurons) were interneurons. Calretinin immunoreactivity was found in a high proportion of neurons (52%). Many of these had TK immunoreactivity. Small calretinin neurons were identified as excitatory neurons to the longitudinal muscle (about 20% of neurons...

Análise morfológica da área de transição do intestino primitivo para o saco vitelino em embriões e fetos bovinos (24 a 50 dias de gestação); Morphologycal analysis of the transition site of the primitive intestine to the yolk sac in bovine embryos and fetal (24 to 50 days gestation)

Mançanares, Celina Almeida Furlanetto
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 19/12/2007 PT
Relevância na Pesquisa
36.6%
O saco vitelino é a principal fonte de nutrição do embrião durante o período em que a placenta verdadeira ainda não está completamente formada. É responsável pela hematopoiese, pois dele se desenvolvem os primórdios das células sanguíneas, bem como parte do sistema circulatório primitivo do embrião. Tivemos como objetivo caracterizar a área transicional do saco vitelino para o intestino primitivo através de análise macroscópica, microscopia de luz, microscopia eletrônica de transmissão e reação imunohistoquímica para detecção de células germinativas (Oct-4). Foram realizados cortes seqüenciais de todos os embriões para permitir a visualização da estrutura como um todo. Nas amostras obtidas, pudemos observar a presença do saco vitelino com a área de conexão com intestino primitivo composto por inúmeras ilhotas vasculares que correspondem aos vasos envolvidos pelas células vitelínicas e células mesenquimais. No interior dos vasos identificamos hemangioblastos em grande quantidade. A camada correspondente ao mesênquima é delgada e com células alongadas e endoderma da membrana vitelínica é composto por células endodérmicas, grandes, uni ou binucleadas. Tal arranjo celular se assemelha a uma glândula devido a sua arquitetura em ilhas vasculares somado às camadas teciduais. O epitélio do intestino primitivo inicia sua diferenciação com células colunares estratificadas e bordadura em escova seguido de células mesenquimais indiferenciadas. A área transicional entre o saco vitelínico e o intestino primitivo é estreita em relação à luz destas duas estruturas com células de formato irregular...

Ação de micro e nanopartículas de dióxido de titânio sobre a resposta inflamatória no intestino delgado de camundongos; Effects of micro and nano-sized titanium dioxide on the inflammatory response on small intestine in mice

Nogueira, Carolina Maciel
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 29/09/2010 PT
Relevância na Pesquisa
36.6%
Introdução: O dióxido de titânio (TiO2) é um corante encontrado na forma de partículas em diversos produtos industrializados. Muitos estudos, a maioria envolvendo o trato respiratório, alertam sobre os efeitos prejudiciais advindos da exposição ao TiO2. Embora partículas da dieta, tais como o TiO2, sejam ingeridas diariamente, ainda existem poucos estudos investigando seus efeitos sobre o trato gastrointestinal. Objetivos: O objetivo principal desse trabalho é investigar a ação de nano (NP) e micropartículas (MP) de TiO2 sobre a resposta inflamatória no intestino delgado de camundongos. Material e Métodos: Camundongos Bl 57/6 foram divididos em 2 grupos experimentais, os quais receberam NP TiO2 (66 nm) ou MP TiO2 (260 nm) a uma dose de 100 mg/Kg/dia, e um grupo controle, o qual recebeu água destilada. O tratamento foi administrado por gavagem, durante 10 dias, uma vez ao dia. Ao final, o intestino delgado foi coletado para a análise de citocinas (IL-1b, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17, IL-23, TNFa, IFNg e TGFb) por ELISA e quantificação de células T CD4+, T CD8+, células dendríticas e natural killers por imunohistoquímica. Resultados: Os resultados demonstram maior concentração de citocinas IL-12...

Caracterização morfoquantitativa do plexo mioentérico do intestino delgado de camundongos mdx : um modelo de distrofia muscular de Duchenne; Morphoquantitative features of myenteric plexus of small intestine of mdx mice: a model for Duchenne muscular dystrophy

Beber, Eduardo Henrique
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 18/08/2011 PT
Relevância na Pesquisa
36.6%
O plexo mioentérico é uma vasta rede de nervos e gânglios localizado entre as camadas longitudinal e circular da túnica muscular externa de todo o trato gastrintestinal (TGI). A distrofia muscular de Duchenne (DMD) é uma miopatia ligada ao cromossomo X causada pela ausência da distrofina que, além dos evidentes efeitos degenerativos no músculo esquelético, causa severas alterações do TGI. No entanto as causas dessas alterações não são claras. Pesquisadores demonstraram que a distrofina é expressa nas fibras musculares lisas e também nos neurônios do plexo mioentérico, todavia não existe um consenso sobre o papel desta nessas estruturas. Desta forma pretende-se estudar os componentes do plexo mioentérico do intestino delgado de camundongos mdx (o modelo animal da DMD) nas idades de 4 e 10 semanas e de seus respectivos controles, camundongos C57BL/10. Os animais de ambos os grupos tiveram o intestino delgado retirado e seccionado em segmentos oral, médio e aboral para posterior avaliação através das técnicas histoquímicas de evidenciação neuronal: NADH-d, NADPH-d e AChE. Além disso, a musculatura lisa e os neurônios do plexo mioentérico foram analisados por MET. A análise quantitativa mostrou que o grupo MDX4 apresentou uma área total do intestino delgado significativamente maior que o C4 (p<0...

Descrição histológica e ultra-estrutural da absorção de óleo de soja pelo intestino do jacaré do pantanal (Caiman yacare, Daudin, 1802).; Histological and ultrastructural description of soybean oil absorption by the intestine of the jacaré do pantanal (Caiman yacare, Daudin, 1802).

Borges, Ricardo Moraes
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 12/09/2014 PT
Relevância na Pesquisa
36.6%
O cultivo de Caiman yacare demanda grandes gastos com alimentação, deste modo, é necessário formular dietas mais baratas e mais eficientes para a engorda destes animais. Uma possibilidade de redução nos custos é o acréscimo de lipídeos de origem vegetal. Neste trabalho, analisou-se o processo de absorção de óleo de soja pelo intestino de Caiman yacare para avaliar possíveis alterações patológicas pelo uso deste óleo na mucosa intestinal de um animal carnívoro. A absorção de óleo de soja ocorreu massiçamente pelo intestino delgado, gerando esteatose intestinal temporária e formando quilomícrons pequenas, que foram transportadas pelo sistema linfático. Outros parâmetros que indicam ausência de patologias na mucosa intestinal, como alteração na proliferação celular, lesão tecidual, recrutamento de granulócitos, formação de heterófilos tóxicos e degranulação de mastócitos, não foram alterados, indicando o potencial uso de óleo de soja na dieta de Caiman yacare.; Cultivation of Caiman yacare demands large financial expenditures on food, so it is desirable to reduce costs by formulating cheaper and more efficient feedings. The soybean oil addition in the diet is an opportunity to reduce spending...

Lactose load enhances the growth of the small intestine in rats

Bicudo, M. H.
Fonte: Inst Tecnologia Parana Publicador: Inst Tecnologia Parana
Tipo: Artigo de Revista Científica Formato: 1005-1011
ENG
Relevância na Pesquisa
36.6%
The present study was undertaken to elucidate the controversial issue regarding the small intestinal structural adaptation, in lactose fed rats. Three study groups were used. One experimental fed 60% lactose and two controls in which the lactose was substituted for similar amount of starch. One of them was fed ad libitum and another a limited amount of food to match the body weight of lactose group. The weight, length of the intestine and intestinal mucosal DNA and protein were determined at days 2, 5, 10 and 30 of the experiment. As compared to starch fed ad libitum controls, animals fed 60% lactose diet ate similar amount of food, grew at a slower rate and weighed 16,7% less at the end of the experiment. In contrast to retarded gain in body weight, small intestinal mucosa of these animals contained more DNA (22,5%) and protein (37,5%) than that of controls. These changes were paralleled by increase in length (17%) and weight of the intestine (24,2%). Therefore, the results of the present study confirmed the findings that the small intestine increases in size in response to lactose feeding and that this occurs in the abscense of hyperphagia. It was further demonstrated that this increase was due both to mucosal cell hyperplasia and hypertrophy.

Walker 256 tumour growth causes marked changes of glutamine metabolism in rat small intestine

Lima, MMR; de Mello, MAR; Curi, R.
Fonte: Wiley-Blackwell Publicador: Wiley-Blackwell
Tipo: Artigo de Revista Científica Formato: 107-113
ENG
Relevância na Pesquisa
36.65%
The effect of Walker 256 tumour growth on the metabolism of glucose and glutamine in the small intestine of rats was examined. Walker 256 tumour has been extensively used as an experimental model to induce cancer cachexia in rats. Walker 256 tumour growth decreased body weight and small intestine weight and length. The activities of glucose-6-phosphate dehydrogenase and phosphate-dependent glutaminase were reduced in the proximal, median and distal portions of the intestine. Glutamine oxidation was reduced in the proximal portion only. The decrease in glutaminase activity was not due to a low synthesis of the protein as indicated by Western blotting analysis. Hexokinase and citrate synthase activities were not changed by the tumour. These findings led us to postulate that tumour growth impairs glutamine metabolism of small intestine but the mechanism involved remains to be elucidated. Copyright (C) 2001 John Wiley Sons, Ltd.

Prolactin regulates luminal bicarbonate secretion in the intestine of the sea bream (Sparus aurata L.)

Ferlazzo, A.; Carvalho, E. S. M.; Gregorio, S. F.; Power, Deborah; Canario, Adelino V. M.; Tischitta, F.; Fuentes, J.
Fonte: Company of Biologists Publicador: Company of Biologists
Tipo: Artigo de Revista Científica
Publicado em 01/11/2012 ENG
Relevância na Pesquisa
36.65%
The pituitary hormone prolactin is a pleiotropic endocrine factor that plays a major role in the regulation of ion balance in fish, with demonstrated actions mainly in the gills and kidney. The role of prolactin in intestinal ion transport remains little studied. In marine fish, which have high drinking rates, epithelial bicarbonate secretion in the intestine produces luminal carbonate aggregates believed to play a key role in water and ion homeostasis. The present study was designed to establish the putative role of prolactin in the regulation of intestinal bicarbonate secretion in a marine fish. Basolateral addition of prolactin to the anterior intestine of sea bream mounted in Ussing chambers caused a rapid (<20min) decrease of bicarbonate secretion measured by pH-stat. A clear inhibitory dose–response curve was obtained, with a maximal inhibition of 60–65% of basal bicarbonate secretion. The threshold concentration of prolactin for a significant effect on bicarbonate secretion was 10ngml–1, which is comparable with putative plasma levels in seawater fish. The effect of prolactin on apical bicarbonate secretion was independent of the generation route for bicarbonate, as shown in a preparation devoid of basolateral HCO3 –/CO2 buffer. Specific inhibitors of JAK2 (AG-490...

Changes in the electrophysiological parameters of the posterior intestine of Anguilla anguilla (Pisces) induced by oxytocin, urotensin II and aldosterone

Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/1997 EN
Relevância na Pesquisa
36.6%
In view of the importance of the intestine in the osmoregulation of freshwater fishes, we determined the effects of oxytocin, urotensin II (UII), and aldosterone added to the serosal side of the isolated posterior intestine of the freshwater-adapted teleost Anguilla anguilla on electrophysiological parameters. Oxytocin decreased the short-circuit current (SCC) and transepithelial potential difference (TPD) at concentrations of 1 and 10 mU/ml (to 50% and 42% of control values, respectively), but did not alter these parameters at a concentration of 0.1 mU/ml. UII reduced SCC and TPD at concentrations of 10 nM, 50 nM and 100 nM (to 85% of control values), but increased these parameters at the concentration of 500 nM (to 115% of control values). Aldosterone did not alter SCC or TPD at the concentrations tested (10 nM and 100 nM). Oxytocin may open Na+ channels in the apical membrane, allowing the flow of Na+ to the serosa, reducing SCC and TPD. Should this hypothesis be correct, oxytocin would be important for freshwater adaptation, since it would increase Na+ absorption. The reduction of SCC and TPD in the posterior intestine of A. anguilla induced by UII is evidence that this neurohormone is also important for freshwater adaptation in teleosts. Aldosterone did not show this effect probably due to the lack of receptors in this organ

Effects of exposure to Diazinon on the lung and small intestine of Guinea pig, histological and some histochemical changes

Rady,Mohamed Ibrahim
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2009 EN
Relevância na Pesquisa
36.6%
The present study aimed to evaluate the histological changes and adverse effects on the total protein and general carbohydrates in the lung and small intestine of guinea pig "Cavia porcellus" induced by the exposure to sub lethal doses of the organophosphate insecticide diazinon. Primarily, diazinon insecticide oral LD50 was investigated after administrating orally different doses in guinea pig and was determined as 213 mg/Kg bw., the animals exposed to sub lethal doses of this insecticide revealed histological changes in both lung and intestine in a dose dependent manner. Also, significant decrease in the total protein and general carbohydrates in the lung and intestine were mostly observed, especially with the higher doses of the insecticide in comparison with control group.

Acute increase, stimulated by prostaglandin E2, in glucose absorption via the sodium dependent glucose transporter-1 in rat intestine

Scholtka, B; Stumpel, F; Jungermann, K
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/1999 EN
Relevância na Pesquisa
36.6%
BACKGROUND/AIMS—Acute stimulation by cAMP of the sodium dependent glucose cotransporter SGLT1 has previously been shown. As prostaglandin E2 (PGE2) increases intracellular cAMP concentrations via its receptor subtypes EP2R and EP4R, it was investigated whether PGE2 could enhance intestinal glucose absorption. 
METHODS—The action of PGE2 on carbohydrate absorption in the ex situ perfused rat small intestine and on 3-O-[14C]methylglucose uptake in isolated villus tip enterocytes was determined. Expression of mRNA for the PGE2 receptor subtypes 1-4 was assayed in enterocytes by reverse transcriptase polymerase chain reaction (RT-PCR). 
RESULTS—In the perfused small intestine, PGE2 acutely increased absorption of glucose and galactose, but not fructose (which is not a substrate for SGLT1); in isolated enterocytes it stimulated 3-O-[14C]methylglucose uptake. The 3-O-[14C]methylglucose uptake could be inhibited by the cAMP antagonist RpcAMPS and the specific inhibitor of SGLT1, phlorizin. High levels of EP2R mRNA and EP4R mRNA were detected in villus tip enterocytes. 
CONCLUSION—PGE2 acutely increased glucose and galactose absorption by the small intestine via the SGLT1, with cAMP serving as the second messenger. PGE2 acted directly on the enterocytes...

Effects of TGF-α gene knockout on epithelial cell kinetics and repair of methotrexate-induced damage in mouse small intestine; Effects of TGF-alpha gene knockout on epithelial cell kinetics and repair of methotrexate-induced damage in mouse small intestine

Xian, C.; Cool, J.; Howarth, G.; Read, L.
Fonte: Wiley-Liss Publicador: Wiley-Liss
Tipo: Artigo de Revista Científica
Publicado em //2002 EN
Relevância na Pesquisa
36.75%
While previous studies have indicated that exogenous TGF-alpha stimulates epithelial growth, maintenance, and repair of the gut, roles of endogenous TGF-alpha are less well-defined particularly in the small bowel. The current study examined effects of TGF-alpha knockout on adult small intestinal epithelial cell proliferation, migration, apoptosis, and damage/repair response after methotrexate treatment. Compared to normal mice, TGF-alpha gene knockout did not affect crypt cell production, mitosis position, migration, and apoptosis in non-injured intestine. RT-PCR gene expression analysis revealed presence of four out of six TGF-alpha related EGF family ligands in the normal intestine, suggesting a possible functional redundancy of the EGF family in maintenance of the intestine. Although TGF-alpha gene knockout did not significantly impair the overall mucosal repair in methotrexate-induced acute damage in the small intestine, it resulted in a higher apoptotic response in the early hours following methotrexate challenge, and a delayed and reduced crypt cell proliferation during repair. Consistently, after methotrexate challenge, intestinal TGF-alpha mRNA was found to be markedly upregulated in the early hours and during repair in the wild type...

Dietry short-chain fructooligosaccharides increase calbindin-D9k levels only in the large intestine in rats independent of dietary calcium deficiency or serum 1,25 dihydroxy vitamin D levels

Takasaki, M.; Inaba, H.; Ohta, A.; Motohashi, Y.; Sakai, K.; Morris, H.; Sakuma, K.
Fonte: Verlag Hans Huber Publicador: Verlag Hans Huber
Tipo: Artigo de Revista Científica
Publicado em //2000 EN
Relevância na Pesquisa
36.79%
Dietary short-chain fructooligosaccharides (Sc-FOS) increase mucosal calbindin-D9k (CaBP) levels in the large intestine whereas levels in the small intestine are decreased in rats. In the present study, we investigated the mechanism by which Sc-FOS induce this increase in CaBP in the large intestine by measuring intestinal CaBP levels in rats fed normal and calcium-deficient diets. Dietary groups included a calcium-containing (0.5%) diet with or without Sc-FOS (100 g/kg diet) and a calcium-deficient (abt. 0.01%) diet with or without Sc-FOS (100 g/kg diet). The rats were fed these diets for 10 days following which they were killed and the intestine removed for collection of the entire mucosa which was divided into four segments, i.e., proximal and distal segments of the small intestine, the cecum and the colorectum. Mucosal CaBP and plasma calcium (Ca), 1,25-dihydroxycholecalciferol (1,25(OH)2D3), 25-hydroxycholecalciferol (25(OH)D3), parathyroid hormone (PTH) and calcitonin levels were measured. Feeding of calcium deficient diet resulted in an increase in CaBP levels in the small intestine, but did not influence levels in the large intestine. Moreover, a significant positive correlation between plasma 1,25(OH)2D3 and CaBP levels in the case of both small intestinal segments (proximal...

IGFBP mRNA expression in small intestine of rat during postnatal development

Shoubridge, C.; Steeb, C.B.; Read, L.
Fonte: Amer Physiological Soc Publicador: Amer Physiological Soc
Tipo: Artigo de Revista Científica
Publicado em //2001 EN
Relevância na Pesquisa
36.75%
In contrast to the adult gut, the immature intestine is refractory to subcutaneously infused insulin-like growth factor I (IGF-I). IGF binding protein (IGFBP) mRNA expression was characterized in intestinal tissues from 6-, 19-, and 90-day-old rats to determine if changes in local expression could account for this age-related change in IGF-I potency. For all age groups, IGFBP-3 to -6, but not IGFBP-1 or -2, were detected by Northern blot analysis. IGFBP-3, -4, and -5 were more intensely expressed in the 6-day-old rat intestine compared with weanling or adult tissue. In contrast, IGFBP-6 expression peaked at the time of weaning. In situ hybridization showed IGFBP-3 to -6 expression was confined to cells of the lamina propria and submucosa and also in the muscularis layer for IGFBP-5. Furthermore, the pattern of IGFBP-5 localization in the intestine changed with development. The findings indicate that the expression of IGFBP-3 to -6 is higher in the immature intestine compared with the adult intestine, suggesting locally produced IGFBPs may inhibit systemically derived IGF-I action in the intestine. Therefore, changes to local IGFBP expression may contribute to the varying response of the rat intestine to IGF-I peptides during postnatal development.

Expression und Funktion arzneimittelmetabolisierender Enzyme und des ABC-Transporters P-Glykoprotein in Dünndarm und Leber des Menschen.; Expression and function of drug metabolizing enzymes and the ABC-transporter P-glycoprotein in human small intestine and liver.

Richter, Oliver von
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
DE_DE
Relevância na Pesquisa
36.6%
Für eine Vielzahl von Arzneimitteln ist die Bioverfügbarkeit nach oraler Gabe gering und weist große interindividuelle Schwankungen auf. Bisher war man davon ausgegangen, daß die Leber das für die präsystemische Elimination von oral applizierten Arzneimitteln entscheidende Organ ist. In jüngster Zeit gibt es jedoch Hinweise darauf, daß auch die Darmwand in erheblichem Umfang zum First-Pass Metabolismus und damit zur unvollständigen und variablen Bioverfügbarkeit von Arzneimitteln beitragen kann. In der vorliegenden Arbeit wurde mittels biochemischer, molekular- und zellbiologischer Techniken die maßgeblich am Phase I Stoffwechsel beteiligten Enzyme der Cytochrom P450 2C- und 3A-Subfamilien, am Phase II Stoffwechsel von Morphin beteiligte UDP-Glucuronosyltransferasen sowie der Arzneimitteltransport durch P-Glykoprotein in aus Dünndarmmukosa isolierten Enterozyten als Mechanismen der oben beschriebenen Variabilität untersucht. Es konnte gezeigt werden, daß Leber und Darm ein unterschiedliches Expressionsprofil für Enzyme des Phase I und Phase II Arzneimittelstoffwechsels aufweisen. CYP3A4 und P-Glykoprotein waren in Enterozyten im Mittel stärker exprimiert als in den entsprechenden Lebern (3,7- bzw. 6,4-fach). CYP2C9 war im Mittel in Enterozyten 9-fach geringer als in den entsprechenden Leberproben exprimiert. In keiner der untersuchten Enterozytenproben konnte CYP2C8 Protein nachgewiesen werden...

Correspondence of gradual developmental increases of expression of galectin-reactive glycoconjugates with alterations of the total contents of the two differentially regulated galectins in chicken intestine and liver as indication for overlapping functions

Lips, K.S.; Kaltner, H.; Reuter, G.; Stierstorfer, B.; Sinowatz, F.; Gabius, H.J.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
36.6%
The duplication of genes for recognition molecules and the ensuing diversification of the members of such families generate complex groups of homologous proteins. One example are galactosidespecific lectins whose sequences display constant features related to sugar binding, the galectins. Based on the inverse abundance of the chicken galectins CG-14 and CG-16 in adult intestine and liver, these two lectins represent a model to comparatively study expression of the related proteins and the galectin-reactive sites (glycoproteins and glycolipids) biochemically and histochemically. Functional overlap andtor acquisition of distinct functions would be reflected in qualitative andlor quantitative aspects of ligand display. Using five different stages of embryogenesis, differential regulation of the two galectins was detected in liver and intestine. The clear preference for one galectin (CC-14) was observed in intestine already at rather early stages, whereas equivalence for both proteins was noted in liver from day 12 to day 18 prior to hatching, as seen by ELISA assays and Western blot analysis. Presentation of galectin-reactive glycoproteins showed a tendency for gradual increase in both organs. Galectin-blotting analysis revealed primarily very similar patterns of positive bands at the different stages of development and only few quantitative and qualitative changes. The reactivity of glycolipids in a solid-phase assay was more variable...

Prognostic significance of metallothionein expression in correlation with Ki-67 expression in adenocarcinomas of large intestine

Dziegiel, Piotr; Forgacz, J.; Suder, E.; Surowiak, P.; Kornafel, Jan; Zabel, M.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
36.65%
The study aimed at determining levels of metallothionein (MT) and Ki-67 antigen expression in adenocarcinomas of large intestine and examining relation of the expression levels with various clinical and pathological variables. The studies were performed on 81 cases of large intestine adenocarcinoma. Using immunocytochemistry, expressions of MT (positive reaction in 73 cases) and of Ki-67 (positive reaction in 79 cases) antigen were examined and the obtained results were compared with, i.a., grade (G) of the tumour and depth to which intestinal wall was infiltrated by individual tumours. Patient survival analysis was also performed, as correlated to expression levels of the two antigens. The obtained results permitted to disclose that the lower was grade of histological differentiation (G2, G3), the more pronounced was expression of MT and Ki-67. Also, the deeper was neoplastic infiltration of intestinal wall, the more pronounced was MT and Ki-67 expression. Despite the relatively strong correlation between MT expression and Ki-67 expression (r=0.536; p<0.05), only Ki-67 antigen expression in large intestine adenocarcinomas was inversely correlated to survival of the patients. Ki-67 proved to be a better prognostic marker...

P-glycoprotein in sheep liver and small intestine: gene expression and transport efflux activity.

Ballent, Mariana; Wilkens, M.R.; Maté, María Laura; Muscher, A.S.; Virkel, Guillermo Leon; Sallovitz, Juan Manuel; Schroder, B.; Lanusse, Carlos Edmundo; Lifschitz, Adrian Luis
Fonte: Wiley-blackwell Publishing, Inc Publicador: Wiley-blackwell Publishing, Inc
Tipo: info:eu-repo/semantics/article; info:ar-repo/semantics/artículo; info:eu-repo/semantics/publishedVersion Formato: application/pdf
ENG
Relevância na Pesquisa
36.6%
The role of the transporter P-glycoprotein (P-gp) in the disposition kinetics of different drugs therapeutically used in veterinary medicine has been demonstrated. Considering the anatomo-physiological features of the ruminant species, the constitutive expression of P-gp (ABCB1) along the sheep gastrointestinal tract was studied. Additionally, the effect of repeated dexamethasone (DEX) administrations on the ABCB1 gene expression in the liver and small intestine was also assessed. The ABCB1 mRNA expression was determined by real-time quantitative PCR. P-gp activity was evaluated in diffusion chambers to determine the efflux of rhodamine 123 (Rho 123) in the ileum from experimental sheep. The constitutive ABCB1 expression was 65-fold higher in the liver than in the intestine (ileum). The highest ABCB1 mRNA expression along the small intestine was observed in the ileum (between 6- and 120-fold higher). The treatment with DEX did not elicit a significant effect on the P-gp gene expression levels in any of the investigated gastrointestinal tissues. Consistently, no significant differences were observed in the intestinal secretion of Rho 123, between untreated control and DEX-treated animals. The understanding of the efflux transporters expression and activity along the digestive tract may help to elucidate clinical implications emerging from drug interactions in livestock.; Fil: Ballent...

Comprimento total do intestino em Bubalus bubalis (Linnaeus, 1758); The length of the intestine in Bubalus bubalis (Linnaeus, 1758)

Borelli, Vicente; Fernandes Filho, Antonio; Ferreira, Nilson; Higashi, Haruo
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; Formato: application/pdf
Publicado em 15/12/1975 POR
Relevância na Pesquisa
36.65%
The length of the various enteric segments as well as the intestine in its total length were studied in 20 adults buffaloes (10 males and 10 females).The results obtained were:small intestine ............................... .27.12 m ± 1.74cecum .............................................. O.43 m ± 0.05colon and rectum ............................. 7.70 m ± 0.53large intestine ................................. 8 .13 m ± 0.58total length ..................................... 32.25 m ± 2.03There was no significant difference concerning sex and intestinal length and no correlation either between the total length of the intestine and the weight and or length of the carcasse.; Os autores estudando o comprimento total do intestino, bem como de seus diversos segmentos, em 20 búfalos de raça não definida, 10 machos e 10 fêmeas, encontraram os seguintes valores:intestino delgado ................................... 27,12 ± 1,74 mceco ...................................................... 0,43 ±  0,05 mcólon e reto ............................................ 7,70 ±  0,53 mintestino grosso ....................................... 8,13 ±  0,58 mcomprimento total ................................. 32,25 ±  2,03 mOs resultados...

Comprimento total do intestino em bovinos de origem Européia; The lenght of the intestine in bovines

Paiva, Orlando Marques de; Borelli, Vicente; Peduti Neto, José
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; Formato: application/pdf
Publicado em 15/09/1977 POR
Relevância na Pesquisa
36.65%
Foi estudado o comprimento dos vários tratos do intestino, bem como seu comprimento total, em bovinos de raças europeias, 20 machos e 20 fêmeas, adultos e encontradas as seguintes medidas: intestino delgado.......................... 39,51m ± 3,09 ceco.............................................. 0,58m ± 0,06 cólon e reto................................... 7,08m ± 0,57 intestino grosso............................. 7,66m ± 0,59 comprimento total ....................... 43,57m ± 2,89 A análise estatística não mostrou diferenças significantes entre os sexos, nem correlação entre o comprimento total do intestino com o peso ou o comprimento da carcaça.; The AA. studied the length of the various enteric tracts as well as their full length in 40 adult bovines of European origin (20 males and 20 females) and achieved the following average, once there was no significant difference concerning sex: thin intestine ....................... 39,51m ± 3,09 caecun .................................. 0,58m ± 0,06 colon and rectun ..................... 7,08m ± 0,57 gross intestine ........................ 7,66m ± 0,59 full length ............................. 43,57m ± 2,89 On the other hand, there was no correlation between the full length of the intestine and the weight or length of the carcasses...