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SEM observation of the bond integrity of fiber-reinforced composite posts cemented into root canals

BONFANTE, Estevam A.; PEGORARO, Luiz F.; GOES, Mario R. de; CARVALHO, Ricardo M.
Fonte: ELSEVIER SCI LTD Publicador: ELSEVIER SCI LTD
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
36.51%
Objectives. To test the null hypothesis that continuity of resin cement/dentin interfaces is not affected by location along the root canal walls or water storage for 3 months when bonding fiber posts into root canals. Methods. Fiber posts were luted to bovine incisors using four resinous luting systems: Multilink, Variolink II, Enforce Dual and Enforce PV. After cementation, roots were longitudinally sectioned and epoxy resin replicas were prepared for SEM analysis (baseline). The original halves were immersed in solvent, replicated and evaluated. After 3 months water storage and a second solvent immersion, a new set of replicas were made and analyzed. The ratio (%) between the length (mm) of available bonding interface and the actual extension of bonded cement/dentin interface was calculated. Results. Significant lower percent values of bond integrity were found for Multilink (8.25%) and Variolink 11 (10.08%) when compared to Enforce Dual (25.11%) and Enforce PV (27.0%) at baseline analysis. The same trend was observed after immersion in solvent, with no significant changes. However, bond integrity was significantly reduced after 3 months water storage and a second solvent immersion to values below 5% (Multilink = 3.31%, Variolink=1.87%...

Avaliação da integridade da superfície no torneamento de um ferro fundido nodular com carboneto. ; Assessment of surface integrity in turning of an ductile cast iron with carbides.

Alvarez Rosário, John Ferney
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 17/04/2006 PT
Relevância na Pesquisa
36.51%
Neste trabalho, estudou-se a integridade superficial durante o torneamento de um ferro fundido nodular com carbonetos, considerando a heterogeneidade microestrutural (fração volumétrica, tamanho e morfologia de grafita e dos carbonetos) e os efeitos dos parâmetros de corte (avanço, velocidade de corte e profundidade de corte), baseados num planejamento fatorial fracionário 24-1. Este texto apresenta, numa primeira parte, uma revisão baseada em referências bibliográficas clássicas da engenharia de superfícies, na tentativa de definir os conceitos que compõem a integridade da superfície. Posteriormente, descrevem-se os resultados de ensaios preliminares, realizados com a finalidade avaliar os níveis do processo e os efeitos desses parâmetros na integridade da superfície. Finalmente, realizaram-se os ensaios de usinagem em corpos-de-prova de ferro fundido nodular com carboneto, nos diâmetros de 80 mm e 140mm. Durante os ensaios de torneamento empregaram-se duas velocidades de corte (60 m/min e 180 m/min), dois avanços (0,1 mm/rev e 0,3 mm/rev) e duas profundidades de corte (0,2 mm e 0,3 mm). Após os ensaios de usinagem, foram realizadas: a avaliação das propriedades do material usinado (dureza e microestrutura), a análise de topografia da superfície usinada mediante avaliação da rugosidade...

Estudo da integridade superficial e seu efeito na fadiga de contato de um aço ferramenta.; The effect of surface integrity on the contact fatigue resistance of a tool steel.

Alvarez Rosário, John Ferney
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 19/09/2011 PT
Relevância na Pesquisa
36.51%
Os processos de manufatura, bem como os parâmetros de processamento utilizados produzem diferentes resultados com relação à integridade de uma superfície. Estas alterações se refletem em mudança das propriedades da superfície, as quais podem influenciar o desempenho dos componentes. O presente trabalho estuda o efeito da integridade superficial gerada pelos processos de torneamento e retificação na resistência à fadiga de contato do aço ferramenta AISI H13. A influência do processo de usinagem e dos parâmetros de corte no estado da superfície e subsuperfície, e como estas características afetam o desempenho são determinados. Foram geradas quatro condições diferentes para as superfícies torneadas mediante a combinação de parâmetros de corte. As superfícies retificadas foram produzidas empregando as mesmas condições de retificação, com isso, obteve-se a mesma condição para todos os corpos de prova ensaiados. As características medidas da integridade superficial foram: os parâmetros de rugosidade, tensões residuais, microdureza Vickers da seção transversal e ruído magnético de Barkhausen. Foi determinado o desempenho das superfícies por fadiga de contato de rolamento, num equipamento na configuração esfera-plano. Os corpos de prova foram anéis de AISI H13 temperados e revenidos. A totalidade dos ensaios foi realizada até a ocorrência de lascamento (Spalling). Os ensaios foram realizados com lubrificação e com uma máxima pressão de contato de Hertz de 3...

O uso da marca sob a ótica da integridade; The trademark use under the perspective of the integrity

Machado, Alexandre Fragoso
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 04/06/2013 PT
Relevância na Pesquisa
36.55%
O presente estudo tem como objetivo analisar os efeitos do uso da marca pelo titular ou por terceiros sob a ótica da integridade. O uso da marca traz consequências negativas ao próprio signo no que tange à sua integridade material, causando o desgaste da sua distinção entre marcas de concorrentes, o que faz diminuir a distância distintiva entre os sinais de uma mesma atividade econômica ou afins. Além disso, o uso da marca também pode causar a perda completa de sua unicidade, corpo distintivo da marca, chegando, às vezes, à vulgarização da mesma, tornando-se res communis omnium. Do outro lado, a integridade material da marca pode passar por processos positivos que fortalecem o seu corpo distintivo. O uso da marca com sucesso faz aumentar sua fama perante o mercado e consumidores, diferenciando-a das demais e sendo alçada ao posto de marca famosa. A fama através do uso impulsiona marcas ordinárias ao status de marcas notoriamente conhecidas ou marcas de alto renome. Ambas representam degraus superiores de integridade material em relação às marcas que são ordinariamente protegidas dentro de suas atividades comerciais. Além disso, apesar do direito de uso exclusivo oferecido às marcas registradas, tal direito não é absoluto. Ou seja...

Integridade biótica dos ecossistemas na região do Arquipélago dos Alcatrazes, São Sebastião - SP; Biotic integrity from the ecosystems of the Alcatrazes archipelago surroundings, São Sebastião - SP

Hoff, Natasha Travenisk
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 23/06/2015 PT
Relevância na Pesquisa
36.51%
A Estação Ecológica (ESEC) Tupinambás, uma UC marinha de proteção integral, está localizada no litoral norte do estado de São Paulo (SP). Foi criada em 1987, sendo utilizada pela Marinha do Brasil para exercícios de tiro até recentemente no arquipélago dos Alcatrazes (São Sebastião, SP). Isto justifica a baixa quantidade de informações disponíveis sobre essa região. Através do levantamento de informações sobre a composição biológica, aspectos socioeconômicos e oceanográficos da área do arquipélago, da utilização de índices ecológicos (dominância, diversidade, riqueza e equabilidade) e multimétricos (Índice ABC e Índice de Integridade Biótica), e da elaboração da Carta de Sensibilidade Ambiental ao Derramamento de Óleo, verificou-se (1) a semelhança da composição ictiofaunística do arquipélago dos Alcatrazes em relação à Santos e São Sebastião, (2) se a região mantém a integridade biótica da ictiofauna mesmo localizando-se entre regiões impactadas, e (3) se o ponto mais sensível ecologicamente detectado pela carta SAO pode ser a área de maior suscetibilidade de ser atingida por um derramamento de óleo. Os dados analisados são provenientes de trabalhos pretéritos e coletas realizadas em setembro de 2011 e janeiro de 2014. A composição ictíica variou ao longo do tempo e períodos de coleta...

SEM observation of the bond integrity of fiber-reinforced composite posts cemented into root canals

BONFANTE, Estevam A.; PEGORARO, Luiz F.; GOES, Mario R. de; CARVALHO, Ricardo M.
Fonte: ELSEVIER SCI LTD Publicador: ELSEVIER SCI LTD
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
36.51%
Objectives. To test the null hypothesis that continuity of resin cement/dentin interfaces is not affected by location along the root canal walls or water storage for 3 months when bonding fiber posts into root canals. Methods. Fiber posts were luted to bovine incisors using four resinous luting systems: Multilink, Variolink II, Enforce Dual and Enforce PV. After cementation, roots were longitudinally sectioned and epoxy resin replicas were prepared for SEM analysis (baseline). The original halves were immersed in solvent, replicated and evaluated. After 3 months water storage and a second solvent immersion, a new set of replicas were made and analyzed. The ratio (%) between the length (mm) of available bonding interface and the actual extension of bonded cement/dentin interface was calculated. Results. Significant lower percent values of bond integrity were found for Multilink (8.25%) and Variolink 11 (10.08%) when compared to Enforce Dual (25.11%) and Enforce PV (27.0%) at baseline analysis. The same trend was observed after immersion in solvent, with no significant changes. However, bond integrity was significantly reduced after 3 months water storage and a second solvent immersion to values below 5% (Multilink = 3.31%, Variolink=1.87%...

Protein kinase C controls activation of the DNA integrity checkpoint

Soriano-Carot, María; Quilis, Inma; Bañó, M. Carmen; Igual, J. Carlos
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
36.51%
The protein kinase C (PKC) superfamily plays key regulatory roles in numerous cellular processes. Saccharomyces cerevisiae contains a single PKC, Pkc1, whose main function is cell wall integrity maintenance. In this work, we connect the Pkc1 protein to the maintenance of genome integrity in response to genotoxic stresses. Pkc1 and its kinase activity are necessary for the phosphorylation of checkpoint kinase Rad53, histone H2A and Xrs2 protein after deoxyribonucleic acid (DNA) damage, indicating that Pkc1 is required for activation of checkpoint kinases Mec1 and Tel1. Furthermore, Pkc1 electrophoretic mobility is delayed after inducing DNA damage, which reflects that Pkc1 is post-translationally modified. This modification is a phosphorylation event mediated by Tel1. The expression of different mammalian PKC isoforms at the endogenous level in yeast pkc1 mutant cells revealed that PKCδ is able to activate the DNA integrity checkpoint. Finally, downregulation of PKCδ activity in HeLa cells caused a defective activation of checkpoint kinase Chk2 when DNA damage was induced. Our results indicate that the control of the DNA integrity checkpoint by PKC is a mechanism conserved from yeast to humans.

A Test of Integrity

Lebsanft, Colette
Fonte: Universidade Nacional da Austrália Publicador: Universidade Nacional da Austrália
Tipo: Relatório
Relevância na Pesquisa
36.69%
An integrity test is an anti-corruption measure used by law enforcement agencies. It involves the creation of a situation or condition that is designed to provoke a reaction by the subject of the test. The subject is allowed to perform, or fail to perform, in a manner consistent with departmental and legislative requirements. How to best combat law enforcement corruption has been a perennial problem for all modern policing bodies. Australian law enforcement agencies have a chequered past but these experiences have resulted in widespread police reform. Australian law enforcement agencies continually strive to improve integrity standards and have developed a range of methods for preventing and detecting corruption. One of the latest moves to strengthen integrity came in 2006 when the Australian Federal Parliament enacted legislation to establish the office of Integrity Commissioner assisted by the Australian Commission for Law Enforcement Integrity (ACLEI). The Australian Federal Police (AFP) has recently announced they are considering implementing an integrity testing regime. Tests take a variety of forms and can be applied on a targeted basis in response to specific suspicions or randomly across an organisation. Regardless of type...

Qiiis custodiet ipsos custodes: can lessons from domestic and overseas experience assist in the implementation of the Law Enforcement Integrity Commissioner Act?

Marwood, Damian
Fonte: Universidade Nacional da Austrália Publicador: Universidade Nacional da Austrália
Tipo: Relatório
Relevância na Pesquisa
36.55%
Law Enforcement corruption is existent throughout the world. Whilst the extent of corruption within any one society is difficult to study, Transparency International annually provide a Corruption Perception Index; a study that surveys the extent of corruption, as perceived by the individuals of the home country. Results from the survey suggest that lower levels of corruption can often be associated lower populated, democratic nations. Regardless of the extent of corruption within these countries, the types of corruption and the factors that contribute to corruption are remarkably similar. Corruption of authority, kickbacks, opportunistic theft, shakedowns, protection of illegal activities, fixing, direct criminal activities, internal payoffs and flaking are types of corruption experienced in all countries studied in this report. While countries such as Australia rank significantly lower on the Corruption Perception Index than countries such as South Africa, the existence of such corruption is still apparent. Factors contributing to corruption such as low wages, promotion prospects, police culture, weak legislation, high community contact, political pressure, poor recruitment standards and inadequate training have all been cited as contributors to corruption from each of the studied countries. Whilst some countries such as Singapore...

Academic Integrity Standards: A Preliminary Analysis of the Academic Integrity Policies at Australian Universities

Bretag, T.; Mahmud, S.; East, J.; Green, M.; James, C.; McGowan, U.; Partridge, L.; Walker, R.; Wallace, M.
Fonte: Australian Universities Quality Agency; Melbourne Publicador: Australian Universities Quality Agency; Melbourne
Tipo: Conference paper
Publicado em //2011 EN
Relevância na Pesquisa
36.69%
As academic integrity is fundamental to assessment practices, it is critical that it is dealt with consistently by staff and taught to students. How a university defines academic integrity in its policy will affect the way it is taught and embedded in the curriculum. While Australian universities all have policy, teaching and learning practices, decision making and review processes relating to academic integrity, these aspects do not always align in a way that reflects a shared understanding of standards of academic integrity, either at intra or inter-university levels. This paper reports on the preliminary findings from the first stage of an ALTC funded project, Academic integrity standards: Aligning policy and practice in Australian universities set to conclude in June 2012. This project seeks to develop a shared understanding across the Australian higher education sector of academic integrity standards with the aim of improving the alignment of academic integrity policies and their implementation. During Stage 1, the project team members analysed the publicly available online academic integrity policies of 39 Australian universities. Our study found that while a significant proportion of academic integrity policies have a punitive element in their approach...

Core elements of exemplary academic integrity policy in Australian higher education

Bretag, T.; Mahmud, S.; Wallace, M.; Walker, R.; James, C.; Green, M.; East, J.; McGowan, U.; Partridge, L.
Fonte: UniSA; Australia Publicador: UniSA; Australia
Tipo: Conference paper
Publicado em //2011 EN
Relevância na Pesquisa
36.58%
This paper reports on one important aspect of the preliminary findings from the Australian Learning and Teaching Council (ALTC) project, Academic integrity standards: Aligning policy and practice in Australian universities. Our project aims to identify approaches to the complex issues of academic integrity, and then to build on these approaches to develop exemplars for adaptation across the higher education sector. Based on analysis of publicly available online academic integrity policies at each of the 39 Australian universities, we have identified five core elements of exemplary academic integrity policy. These have been grouped under the headings, Access, Approach, Responsibility, Detail and Support, with no element given priority over another. In this paper we compare the five core elements identified in our research with best practice guidelines recommended by the Higher Education Academy (HEA) in the UK. We conclude that an exemplar policy needs to provide an upfront, consistent message, reiterated throughout the entire policy, which indicates a systemic and sustained commitment to the values of academic integrity and the practices that ensure it. Whereas the HEA created two discrete resources, the key aim and challenge of this project will be to develop exemplars that demonstrate a strong alignment between policy and practice.; http://www.apcei.catl.uwa.edu.au/procs; Tracey Bretag...

Impact of potassium permanganate on cyanobacterial cell integrity and toxin release and degradation

Fan, J.; Daly, R.; Hobson, P.; Ho, L.; Brookes, J.
Fonte: Pergamon-Elsevier Science Ltd Publicador: Pergamon-Elsevier Science Ltd
Tipo: Artigo de Revista Científica
Publicado em //2013 EN
Relevância na Pesquisa
36.51%
Potassium permanganate (KMnO4) is commonly used as a pre-treatment oxidant to remove soluble manganese (Mn) and iron (Fe) which can contribute to dirty water in drinking water supplies. Because Mn and Fe problems are commonly associated with thermal stratification in summer and autumn, they frequently coincide with the presence of cyanobacteria. The use of KMnO4 as an oxidant for Mn and Fe control therefore needs to consider the potential impacts on cyanobacterial cell integrity and toxin release. This study aims to assess the effect of KMnO4 on cyanobacteria cell integrity, toxin release and toxin oxidation. A toxic strain of Microcystis aeruginosa was exposed to various concentrations of KMnO4 and the cell integrity of cyanobacteria was measured with flow cytometry. Further the intra- and extra-cellular toxin concentrations were quantified and it was apparent that KMnO4 reduced both the intra- and extra-cellular toxins at low initial concentrations of 1 and 3 mg L(-1) without complete cell lysis. However, the cell integrity of cyanobacteria was compromised at KMnO4 concentrations of 5 mg L(-1) and 10 mg L(-1) and led to intracellular toxin release. In the 10 mg L(-1) KMnO4 treatment, the total toxin was oxidised after 7h contact time. A model describing the two step process of release and degradation was developed and may provide a tool to assess the risk water quality posed by toxin release. Consequently...

Rising to the Challenge: Critiquing the Australian Commission for Law Enforcement Integrity (ACLEI) in preventing misconduct in the Australian Federal Police International Deployment Group (AFP IDG)

Santelises, Aaron
Fonte: Universidade Nacional da Austrália Publicador: Universidade Nacional da Austrália
Tipo: Relatório
Relevância na Pesquisa
36.55%
Due to this globalised world and Australia's commitment to peacekeeping and law enforcement, we have committed ourselves to deploying our police overseas in the program known as the Australian Federal Police International Deployment Group (AFP IDG). With this deployment, come consequences such as corruption and the vulnerability of ruining Australia's intentional prestige. Thus, it's the integrity institutions responsibility to make sure that this doesn't occur. One of those institutions is the subject of this report, which is the Australian Commission for Law Enforcement Integrity (ACLEI), an institution run by the Integrity Commissioner, investigates any form of corruption, misconduct or perversion of justice by the Australian Federal Police (AFP), Australian Crime Commission or Customs officials within Australia. However, with the AFP IDG, deployed overseas, ACLEI will have to perform investigations overseas if the need ever arises. This report aims to ascertain whether ACLEI is fully prepared to conduct an investigation overseas, for the AFP IDG. A comparing and contrasting method will be utilised, by reviewing statute, legislative branch committee meetings and speeches from USA and UK case studies. Maintaining integrity is vital for any law enforcement agency to ensure that they are perceived as agents of the state that enforce the rule of law and not a group of individuals that exploit their power. This is paramount...

Integrity-Based Kernel Malware Detection

Zhu, Feng
Fonte: FIU Digital Commons Publicador: FIU Digital Commons
Tipo: Artigo de Revista Científica Formato: application/pdf
Relevância na Pesquisa
36.62%
Kernel-level malware is one of the most dangerous threats to the security of users on the Internet, so there is an urgent need for its detection. The most popular detection approach is misuse-based detection. However, it cannot catch up with today's advanced malware that increasingly apply polymorphism and obfuscation. In this thesis, we present our integrity-based detection for kernel-level malware, which does not rely on the specific features of malware. We have developed an integrity analysis system that can derive and monitor integrity properties for commodity operating systems kernels. In our system, we focus on two classes of integrity properties: data invariants and integrity of Kernel Queue (KQ) requests. We adopt static analysis for data invariant detection and overcome several technical challenges: field-sensitivity, array-sensitivity, and pointer analysis. We identify data invariants that are critical to system runtime integrity from Linux kernel 2.4.32 and Windows Research Kernel (WRK) with very low false positive rate and very low false negative rate. We then develop an Invariant Monitor to guard these data invariants against real-world malware. In our experiment, we are able to use Invariant Monitor to detect ten real-world Linux rootkits and nine real-world Windows malware and one synthetic Windows malware. We leverage static and dynamic analysis of kernel and device drivers to learn the legitimate KQ requests. Based on the learned KQ requests...

Influence of cutting process mechanics on surface integrity and electrochemical behavior of OFHC copper

DENGUIR, Lamice; OUTEIRO, José; FROMENTIN, Guillaume; VIGNAL, Vincent; BESNARD, Rémy
Fonte: ELSEVIER Publicador: ELSEVIER
EN
Relevância na Pesquisa
36.51%
The authors gratefully acknowledge the support received from IC ARTS and CEA Valduc; Superfinishing machining has a particular impact on cutting mechanics, surface integrity and local electrochemical behavior. In fact, material removal during this process induces geometrical, mechanical and micro-structural modifications in the machined surface and sub-surface. However, a conventional 3D cutting process is still complex to study in terms of analytical/numerical modeling and experimental process monitoring. So, researchers are wondering if a less intricate configuration such as orthogonal cutting would be able to provide information about surface integrity as close as possible to that one generated by a 3D cutting process. For that reason, in the present paper, two different machining configurations were compared: face turning and orthogonal cutting. The work material is oxygen free high conductivity copper (OFHC) and the cutting tools are uncoated cemented carbide. The research work was performed in three steps. In the first step, the process mechanics of superfinishing machining of OFHC copper was performed. In the second step, the surface integrity and the chemical behavior of the machined samples were analyzed. Finally, in the third step...

Evaluation of stream ecological integrity using litter decomposition and benthic invertebrates

Castela, José; Ferreira, Verónica; Graça, Manuel A. S.
Fonte: Universidade de Coimbra Publicador: Universidade de Coimbra
Tipo: Artigo de Revista Científica Formato: aplication/PDF
ENG
Relevância na Pesquisa
36.58%
Biomonitoring programs to access the ecological integrity of freshwaters tend to rely exclusively on structural parameters. Here we evaluated stream ecological integrity using (a) benthic macroinvertebrate derived metrics and a biotic index as measures of structural integrity and (b) oak litter decomposition and associated fungal sporulation rates as measures of functional integrity. The study was done at four sites (S1, S2, S3 and S4) along a downstream increasing phosphorus and habitat degradation gradient in a small stream. The biotic index, invertebrate metrics, invertebrate and fungal communities' structure and sporulation rates discriminated upstream and downstream sites. Decomposition rates classified sites S4 and S2 as having a compromised ecosystem functioning. Although both functional and structural approaches gave the same results for the most impacted site (S4), they were complementary for moderately impacted sites (S2 and S3), and we therefore support the need for incorporating functional measures in evaluations of stream ecological integrity.; http://www.sciencedirect.com/science/article/B6VB5-4PXG7N9-1/1/9b2636d1de504e67a3370feb1b137b52

Cyclic load testing for integrity evaluation of prestressed concrete girders

Barrios F.; Ziehl P.H.
Fonte: Universidade de Medellín Publicador: Universidade de Medellín
Tipo: Article; info:eu-repo/semantics/article
ENG
Relevância na Pesquisa
36.6%
This study is focused on the performance and validation of the 24-hour load test (24h LT) method and the cyclic load test (CLT) method as applied to full-scale lightweight and normalweight self-consolidating prestressed concrete girders. This study examines data obtained from the four-point loading tests of six full-scale T-girders and applies the current criteria from these methodologies to evaluate the presence of damage and structural integrity. The experimental results indicate that the recovery criteria of the 24h LT method were insensitive to damage and hence did not provide a satisfactory integrity assessment of the members. Also, the permanency and repeatability criteria of the CLT were insensitive to damage for the girders studied. The global integrity parameter (GIP) based on the deviation from linearity criterion from the CLT is proposed for the quantitative assessment of the level of damage in prestressed concrete girders, and the results indicate good correlation with the experimental data. © 2012, American Concrete Institute.

Methods of high integrity RNA extraction from cell/agarose construct

Ogura, Takahiro; Tsuchiya, Akihiro; Minas, Tom; Mizuno, Shuichi
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
EN_US
Relevância na Pesquisa
36.55%
Background: Agarose hydrogels are widely used for three-dimensional cell scaffolding in tissue engineering and cell biology. Recently, molecular profiles have been obtained with extraction of a minimal volume of RNA using fluorescent-tagged quantitative polymerase chain reaction (qPCR), which requires high integrity RNA. However, the agarose interferes considerably with the quantity and quality of the extracted RNA. Moreover, little is known about RNA integrity when the RNA is extracted from cell/agarose construct. Thus, in order to obtain RNA of sufficient integrity, we examined various extraction methods and addressed reproducible methodologies for RNA extraction from cell/agarose constructs using spectrophotometry and microfluidic capillary electrophoresis. Results: With various extraction methods using a mono-phasic solution of phenol and guanidine isothiocyanate, we evaluated quantity and quality of total RNA from cell/agarose construct. Extraction with solution of phenol and guanidine isothiocyanate followed by a silica based membrane filter column gave sufficient RNA integrity number, which allowed us to proceed to fluorescent-tagged qPCR for evaluating various cellular activities. Conclusions: The RNA extraction methods using phenol and guanidine isothiocyanate solution and a silica membrane column can be useful for obtaining high integrity RNA from cell/agarose constructs rich in polysaccharide and extracellular matrix. Our study contributes to further investigation using agarose hydrogels and other materials rich in polysaccharide in the field of cellular and tissue engineering.

Comparação entre o índice de integridade biótica e um método de multicritério, para análise da qualidade ambiental de três riachos tributários ao reservatório de Itaipu; Comparison between the index of biotic integrity and a method of multicriteria for analysis of the environmental quality of three streams tributaries to the of Itaipu reservoir; Comparison between the index of biotic integrity and a method of multicriteria for analysis of the environmental quality of three streams tributaries to the of Itaipu reservoir

Fernandes, Cleodimar; UFPR; Volpi, Neida Maria Patias; UFPR; Baumgartner, Gilmar; UNIOESTE
Fonte: Universidade Federal Fluminense - LATEC Publicador: Universidade Federal Fluminense - LATEC
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; Formato: application/pdf
Publicado em 27/05/2009 POR
Relevância na Pesquisa
36.62%
As atividades humanas têm exercido profunda e, normalmente, negativa influência sobre oscorpos hídricos, sendo que alguns efeitos negativos são devidos aos poluentes, enquantooutros estão associados às mudanças na hidrologia da bacia, modificações no habitat ealterações das fontes de energia. Muitas situações na tomada de decisão, sobre umambiente, envolvem a seleção de alternativas, eventos ou cursos de ações. No entanto,essas decisões geralmente possuem pontos de vista conflitantes, com diferentes juízos devalores, forçando os tomadores de decisão a decidirem sem uma base técnica suficiente,induzindo muitas vezes a decisões equivocadas. Em vista do exposto acima, o objetivodesta pesquisa foi apresentar um método de multicritério (ELECTRE TRI), para avaliar aqualidade ambiental de riachos e compará-lo ao Índice de Integridade Biótica, sendo queambos foram aplicados em afluentes ao Reservatório de Itaipu (rios Ajuricaba, Arroio Fundoe Curvado), utilizando-se a comunidade de peixes como indicador. De acordo com osresultados constatou-se que a escolha do método multicritério para este tipo de trabalho éválida, pois quando comparados com o Índice de Integridade Biótica, os resultados não sedistanciaram. Deste modo...

A conceptual framework of integrity

Barnard,Antoni; Schurink,Willem; De Beer,Mariè
Fonte: SA Journal of Industrial Psychology Publicador: SA Journal of Industrial Psychology
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2008 EN
Relevância na Pesquisa
36.58%
This article reports on the findings of a qualitative study in which the construction of integrity of some business leaders was explored. Data were gathered through ten in-depth interviews with six South African business leaders commended to be champions of integrity. A grounded-theory approach to the data analysis elicited five themes. These themes and their interrelatedness are discussed in this article and a conceptual framework of integrity is proposed. Integrity is conceptualised as a multifaceted and dynamic construct based on a moral foundation and inner drive that is managed by cognitive and affective processes manifesting various integrity-related behaviours.