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Hydrolysis Influence on Phytochemical Composition, Antioxidant Activity, Plasma Concentration, and Tissue Distribution of Hydroethanolic Ilex paraguariensis Extract Components

RIVELLI, Diogo P.; ALMEIDA, Rebeca L.; ROPKE, Cristina D.; BARROS, Silvia B. M.
Fonte: AMER CHEMICAL SOC Publicador: AMER CHEMICAL SOC
Tipo: Artigo de Revista Científica
ENG
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The infusion of aerial parts of Ilex paraguariensis is widely consumed. Its antioxidant activity suggests an important role of this plant in the treatment/prevention of oxidative stress related diseases. Plant extract active compounds are frequently found in esterified form that may be poorly absorbed. Hydrolysis of the extract is a possible approach to increase its bioavailability. The aim of this study was to perform a phytochemical analysis and evaluate in rats the plasma concentration and tissue distribution of antioxidant compounds in the hydroethanolic extract of Ilex paraguariensis, before and after enzymatic hydrolysis. Both extracts presented high antioxidant activity and phenolic content. Rats given single or repeated doses of the hydrolyzed extract showed increased plasma antioxidant activity and higher plasma levels of caffeic acid. However, no changes of endogenous antioxidants were observed. In conclusion, hydrolysis of the extract of Ilex paraguariensis is a strategy to improve its bioavailability and in vivo antioxidant activity.; Fapesp (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)

Oral ingestion of a hydrolyzed gelatin meal in subjects with normal weight and in obese patients: Postprandial effect on circulating gut peptides, glucose and insulin

RUBIO, I. G. S.; CASTRO, G.; ZANINI, A. C.; MEDEIROS-NETO, G.
Fonte: EDITRICE KURTIS S R L Publicador: EDITRICE KURTIS S R L
Tipo: Artigo de Revista Científica
ENG
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Gut hormones Ighrelin, peptide YY (PYY) and ghrcagon-like peptide-1 (GLP-1)] are an important group of hormones that target appetite control. They are released from endocrine L cells of the small bowel in proportion to the volume, components and calories in a meal. In the current study, 20 g of gelatin (flavored and sweetened) were given to obese patients (n=12) and lean subjects (n=10). Subsequently, plasma samples were collected at-30-minute intervals rip to 180 minutes and glucose, insulin, PYY, GLP-1 and ghrelin were assayed using specific and sensitive immunofluorometric and radioimmunoassays. As expected, obese patients had normal serum glucose levels, higher serum insulin, and lower plasma concentration of ghrelin at all times compared to lean subjects. GLP-1 plasma levels were significantly elevated at 60 minutes, peaking at 120 minutes in obese patients and lean subjects. As a consequence, there was a significant rise in serum insulin levels with a significantly higher peak level at 60 min (obese) and 30 min (lean). There were no significant changes in PYY plasma concentrations and no correlation was found between body mass index and concentrations of ghrelin, PYY and GLP-1 in the group of obese patients. In conclusion, a single gelatin meal induces a rise in plasma GLP-1 followed by an increase in serum levels of insulin. These findings may be applied to maximize satiety in obese patients as a means of improving adherence to calorie-controlled diets as well as provide better control of diabetic patients.

Digestibilidade enzimática do bagaço de cana-de-açucar tratado quimio-mecanicamente; Enzimatic digestibility of chemomechanical pretreated sugarcane bagasse

Mendes, Fernanda Machado
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 21/12/2010 PT
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Métodos que convertem o bagaço de cana-de-açúcar em açúcares fermentescíveis são geralmente compostos por duas etapas principais: pré-tratamento para degradar a estrutura da planta e uma etapa de hidrólise enzimática ou química para converter as cadeias poliméricas em açúcares. Cada tecnologia de pré-tratamento tem um mecanismo diferente de ação sobre a estrutura do bagaço induzindo modificações físicas e/ou químicas, que são necessárias devido a presença de hemicelulose e lignina na parede celular da planta, o que impede o acesso das celulases nas partes internas do substrato. No presente trabalho, o processo quimio-mecânico foi utilizado para pré-tratar o bagaço de cana com o objetivo de aumentar a acessibilidade na parede celular pelas enzimas hidrolíticas. O processo associa a vantagem da remoção de um ou mais componentes do bagaço e o aumento da área superficial por trituração. Após o tratamento quimio-mecânico, rendimento do processo com adição de álcali foi de 91% e com sulfito alcalino de 75%, que corresponde à remoção de 33% e 53% de lignina e 13% e 29% da hemicelulose, respectivamente.A conversão de celulose de amostras pré-tratadas com álcali e sulfito-alcalino atingiu 50% e 85%...

Efeito da hidrólise enzimática seguida da moagem em moinho de bolas sobre as características estruturais e físico-químicas do amido de madioquinha-salsa

Conceição, Flaviana Sales
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Dissertação de Mestrado Formato: 71 f. : il. color.
POR
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE; Amidos modificados são utilizados pela indústria de alimentos por apresentarem melhor comportamento que amidos nativos. Modificações visando a redução do diâmetro granular para a obtenção de grânulos de tamanho similar aos da molécula de gordura tem sido de grande interesse, pois este amido modificado pode ser usado como substituto de gordura em diversas formulações. Para isso, o pré-tratamento enzimático pode ser usado para fragilizar a estrutura granular do amido e facilitar um posterior tratamento físico como a moagem, obtendo grânulos com menor diâmetro. O objetivo deste trabalho foi investigar o efeito da hidrólise enzimática seguida de moagem em moinho de bolas sobre as características estruturais e físico-químicas do amido de mandioquinha-salsa (Arracacia xanthorrhiza). Amido isolado de raízes de mandioquinha-salsa foi hidrolisado com α-amilase bacteriana e/ou amiloglucosidase fúngica, a 37 o C, em três diferentes condições: A: 40 SKB/g de α-amilase e 10 U/g amiloglucosidase por 36 h; B: 20 SKB/g de α-amilase e 5 U/g amiloglucosidase por 12 h; C: 5 U/g amiloglucosidase por 12 h. Após hidrólise os mesmos foram moídos por 1 e 2 h. Os amidos nativos e modificados foram analisados quanto à distribuição de tamanho de grânulos. Houve redução do diâmetro dos grânulos para todos os amidos tratados...

Desenvolvimento de processo enzimatico para redução de sedimentos em extratos de cafe soluvel; Development of enzymatic process for sediments reduction in coffe extracts

Paula Aparecida Delgado
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 09/05/2008 PT
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Polissacarídeos são os principais constituintes do café verde, torrado e solúvel, sendo principalmente galactomananos e arabinogalactanos. Um aspecto importante em relação a estes polissacarídeos é a insolubilidade, umas das possíveis razões para a formação de sedimentos na produção de café solúvel, reduzindo o rendimento do processo. Em face disto, o objetivo deste trabalho foi investigar a redução de sedimentos em extrato de café pela ação de enzimas. Diversas preparações comerciais de pectinases foram selecionadas e suas diferentes atividades determinadas. Rohapect B1L apresentou a maior atividade de mananase. Adicionalmente, a maioria das preparações estudadas apresentou outras atividades enzimáticas, tais como mananase, endoglucanase, xilanase, além da atividade atestada pelos fabricantes. Frações solúveis e insolúveis do extrato de café e sedimento foram preparadas e suas composições determinadas. Os principais componentes do extrato de café e sua fração insolúvel foram carboidratos e proteínas, enquanto que a fração insolúvel do sedimento apresentou também uma fração significativa de lignina. Pela análise dos carboidratos, galactose, arabinose e manose foram os principais monossacarídeos obtidos após hidrólise ácida...

Efeito do consumo de hidrolisado do soro de leite no metabolismo energético e no estado redox de ratos sedentários e exercitados; Effect of the intake of hydrolyzate whey proteins on energy metabolism and redox state of sedentary and exercised rats

Daniela Gasparetto
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 06/12/2011 PT
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As proteinas do soro de leite possuem alto valor nutritivo, sendo, portanto, extensamente estudadas em diversas areas do saber. A equipe do Laboratorio de Fontes Proteicas vem estudando a associacao entre o consumo do hidrolisado de proteina do soro de leite e seus efeitos biologicos e nutricionais, em varios niveis de atividade fisica. Seu consumo tem sido associada a diminuicao do estresse metabolico, reducao nos niveis de lactato, aumento das reservas de glicogenio muscular, maior estabilidade da albumina serica e melhora nos tempos de exaustao do animal treinado. Este trabalho teve por objetivo avaliar o efeito do consumo do hidrolisado de soro de leite no estado metabolico redox do rato e na utilizacao de lipideos pelo organismo como fonte de energia durante a atividade fisica. Ratos Wistar machos foram divididos em 3 grupos de dieta: Padrao (AIN 93-G, dieta elaborada com caseina), Controle (AIN 93-G, elaborada com concentrado de proteinas do soro de leite) e Experimental (AIN 93-G, elaborada com hidrolisado de proteinas do soro de leite). Cada dieta foi subdividida em 4 grupos (n = 7): sedentarios, sedentarios-exaustos, treinados e treinados-exaustos. O hidrolisado apresentou maior poder antioxidante in vitro, do que o concentrado e tres fracoes do soro...

Biotransformação do óleo de rícino em aromas por Yarrowia lipolytica

Braga, Adelaide
Fonte: Universidade do Minho Publicador: Universidade do Minho
Tipo: Trabalho de Conclusão de Curso
Publicado em //2009 POR
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Dissertação de mestrado integrado em Engenharia Biológica; Os compostos aromáticos produzidos por processos biotecnológicos são cada vez mais aceites pelo mercado consumidor, por serem considerados naturais. Além disso, são processos de grande interesse devido aos elevados rendimentos que apresentam relativamente aos processos de extracção a partir de fontes naturais. A γ-decalactona é um composto aromático de interesse industrial, que resulta da β-oxidação peroxisomal do ácido ricinoleico. Este ácido gordo, maior constituinte do óleo de rícino, é o precursor mais vulgarmente utilizado na produção biotecnológica deste aroma. Para que o substrato (óleo de rícino) esteja mais disponível para as células o utilizarem na produção de γ-decalactona, pode utilizar-se óleo de rícino hidrolisado. Esta hidrólise pode ser promovida por acção enzimática, mais especificamente por lipases. O objectivo geral do presente trabalho consiste na avaliação do papel das lipases, comerciais e produzidas pela levedura, na hidrólise do óleo de rícino e consequente impacto na produção de γ-decalactona. Inicialmente foi feita a validação do método experimental utilizado para determinar a actividade lipolítica...

Comparative Analysis of the Chemical Composition of Mixed and Pure Cultures of Green Algae and Their Decomposed Residues by 13C Nuclear Magnetic Resonance Spectroscopy

Zelibor, J. L.; Romankiw, L.; Hatcher, P. G.; Colwell, R. R.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/1988 EN
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It is known that macromolecular organic matter in aquatic environments, i.e., humic substances, is highly aliphatic. These aliphatic macromolecules, predominantly paraffinic in structure, are prevalent in marine and lacustrine sediments and are believed to originate from algae or bacteria. A comparative study of mixed and pure cultures of green algae and their decomposed residues was performed by using solid-state 13C nuclear magnetic resonance spectroscopy as the primary analytical method. Results obtained in this study confirm the presence of components that are chemically refractory and that are defined as alghumin and hydrolyzed alghumin. These were detected in heterogeneous, homogeneous, and axenic biomasses composed of several genera of Chlorophyta. Although the chemical composition of algal biomass varied with culture conditions, the chemical structure of the alghumin and hydrolyzed alghumin, demonstrated by 13C nuclear magnetic resonance spectroscopy appeared to be constant for members of the Chlorophyta examined in this study. The alghumin was dominated by carbohydrate-carbon, with minor amounts of amide or carboxyl carbon and paraffinic carbon, the latter surviving strong hydrolysis by 6 N HCI (hydrolyzed alghumin). Bacterial decomposition of heterogeneous algal biomass labeled with 13C was conducted under both aerobic and anaerobic conditions to determine chemical structure and stability of the refractory material. The refractory fraction ranged from 33% in aerobic to 44% in anaerobic cultures. The refractory fraction recovered from either aerobic or anaerobic degradation comprised 40% alghumin...

Purification of the influenza hemagglutinin glycoprotein and characterization of its carbohydrate components.

Collins, J K; Knight, C A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1978 EN
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Hemagglutinin from influenza A/PR8 virus was purified after treatment of the virus with sodium deoxycholate followed by extraction with tri-n-butyl phosphate. This fully disrupted the virus while preserving hemagglutinating activity. The hemagglutinin was obtained in the form of small aggregates that could be separated from other viral components. Purified hemagglutinin was hydrolyzed to determine carbohydrate composition and digested with Pronase to analyze oligosaccharide structures. Sugars present in the hemagglutinin were galactose, mannose, fucose, and glucosamine in molar rates of about 6:11:2:5, and these comprised 16% of the hemagglutinin glycoprotein. Oligosaccharides obtained from virus included a major component of a molecular weight of 2,800, composed of glucosamine, galactose, mannose, and fucose, and a minor heterogenous component of a molecular weight of 1,500 to 2,000, containing predominantly mannose. The 2,800-molecular-weight oligosaccharide was a constituent of the hemagglutinin, and treatment of this large oligosaccharide with specific exo-glycosidases demonstrated the presence of terminal galactose and fucose and allowed the deduction of a general structure for this component.

Electron Paramagnetic Resonance of Nitrogenase and Nitrogenase Components from Clostridium pasteurianum W5 and Azotobacter vinelandii OP

Orme-Johnson, W. H.; Hamilton, W. D.; Jones, T. L.; Tso, M.-Y. W.; Burris, R. H.; Shah, V. K.; Brill, W. J.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1972 EN
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The electron paramagnetic resonance of nitrogenase components, separately and together with the other reactants in the nitrogenase system (namely, reductant and Mg·ATP), have been examined at low temperatures (<20°K). The MoFe protein, component I or molybdoferredoxin, in the oxidized (but not oxygen-inactivated) state yields signals with g-values of 4.3, 3.7, and 2.01, and when reduced has no observable electron paramagnetic resonance. The Fe protein, component II, or azoferredoxin, yields a signal with g-values of 2.05, 1.94, and 1.89 in the reduced state that is converted by Mg·ATP into an axial signal with g-values near 2.05 and 1.94, and a second split signal near g = 4.3. The Fe protein has no definite electron paramagnetic resonance in the oxidized (not oxygen-denatured) state under these conditions. The Mg·ATP complex of reduced Fe protein reduces the MoFe protein, whereas dithionite alone does not reduce the MoFe protein. Reoxidation of the system by substrate leads to disappearance of the Fe protein signal and the reappearance of the MoFe protein signal. Thus Mg·ATP, which is hydrolyzed during substrate reduction, converts the Fe protein to a reductant capable of transferring electrons to MoFe protein, after which substrate reduction occurs.

Membrane-bound neuraminidase from calf brain: regulation of oligosialoganglioside degradation by membrane fluidity and membrane components.

Sandhoff, K; Pallmann, B
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1978 EN
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25.84%
The degradation of lipophilic ganglioside GD1a and hydrophilic sialyllactitol by membrane-bound neuraminidase (EC 3.2.1.18) from calf brain has been studied at substrate concentrations of 0.1 mM. Ganglioside GD1a taken up by cell membranes is hydrolyzed faster membrane-bound neuraminidase than are water-soluble substrates of the enzyme, sialyllactitol and des-GD1a. Availability and enzymic breakdown of the disialoganglioside are enhanced by general anesthetics such as N2O or halothane whereas the degradation of the hydrophilic substrate silayllactitol is not affected or even is decreased by these agents. General anesthetics lower the microviscosity of membranes as indicated by studies of fluorescence depolarization with the indicator 1,6-diphenylhexatriene. Increased fluidity can result in higher lateral diffusion of ganglioside GD1a, thus increasing its chances of presentation to, and interaction with, membrane-bound neuraminidase. Lipophilic derivatives of the disialoganglioside, gangliosides GM1 and GM2 and gangliotriaosylceramide GA2, are strong inhibitors of the ganglioside degradation whereas water-soluble derivatives des-GM1, des-GM2, N-acetylneuraminic acid, and sialyllactose are not. A model is presented that suggests that the activity of membrane-bound neuraminidase on gangliosides of brain membranes is regulated by the viscosity of these membranes and their monosialoganglioside content.

Direct evidence for the presence of lipopolysaccharide components in Pseudomonas ribosomal vaccine.

Lieberman, M M
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /08/1977 EN
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25.84%
The presence of sugars specific to lipopolysaccharide, glucose, and rhamnose was demonstrated in a Pseudomonas ribosomal vaccine. The detection of these sugars was accomplished by radiological means after paper chromatography of the neutral fraction of acid-hydrolyzed vaccine.

Studies on Inhibition of the Intestinal Absorption of Radioactive Strontium: IX. Relationship Between Biological Activity and Electron Microscopic Appearance of Alginic Acid Components

Tanaka, Y.; Inoue, S.; Skoryna, S. C.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 12/09/1970 EN
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The inhibitory action of alginate on intestinal absorption of radioactive strontium was investigated in order to correlate the biological activity with the chemical composition. Alginate from Laminaria hyperborea was partially hydrolyzed with oxalic acid and the degradation products were fractionated into polymannuronic and polyguluronic acid. The activity of these products was assessed biologically in rats and morphologically by electron microscopy. Sodium polymannuronate was found to be less effective than sodium polyguluronate in preventing absorption of radiostrontium. The inhibition of absorption of radio-calcium was low and not affected by hydrolysis or fractionation. When dried from dilute aqueous solutions, the polymannuronate retained the original helical structure of alginate, while the polyguluronate showed a strong tendency to coagulate, forming granules. The variation in the biological activity was attributed to the morphological differences between these alginic acid components and it is suggested that the degree of uncoiling of the polyguluronate chain in water is greater than that of the polymannuronate chain, thus making the carboxylate ions more accessible to strontium.

Cyclodextrin-modified micellar electrokinetic chromatography for the analysis of Esterom®, a topical product consisting of hydrolyzed benzoylecgonine in propylene glycol

Razak, Jennifer L.; Doyen, Heidi J.; Lunte, Craig E.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /06/2003 EN
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25.95%
Esterom®, a new drug currently in human clinical trials, is a mixture of compounds in a propylene glycol vehicle. It is being evaluated as a topical treatment to aid in the relief of muscle pain and to increase range of motion. Benzoylecgonine is the major component of Esterom and there are at least nine other minor constituents, including four hydroxypropyl esters that have multiple diasteriomers. The aim of the study was to develop a capillary electrophoresis method for the simultaneous separation of the main components in Esterom, including the multiple proposed diastereomers of the esters. Due to the complex sample composition, the use of micelles and cyclodextrins as buffer modifiers was evaluated. A cyclodextrin-modified micellar electrokinetic chromatography method was able to determine 7 of the 8 UV-active Esterom components, with baseline separation of 7 of the 10 diastereomers of the hydroxypropyl esters.

Organic nitrogen components in soils from southeast China*

Chen, Xian-you; Wu, Liang-huan; Cao, Xiao-chuang; Zhu, Yuan-hong
Fonte: Zhejiang University Press Publicador: Zhejiang University Press
Tipo: Artigo de Revista Científica
Publicado em /04/2013 EN
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Objective: To investigate the amounts of extractable organic nitrogen (EON), and the relationships between EON and total extractable nitrogen (TEN), especially the amino acids (AAs) adsorbed by soils, and a series of other hydrolyzed soil nitrogen indices in typical land use soil types from southeast China. Under traditional agricultural planting conditions, the functions of EON, especially AAs in the rhizosphere and in bulk soil zones were also investigated. Methods: Pot experiments were conducted using plants of pakchoi (Brassica chinensis L.) and rice (Oryza sativa L.). In the rhizosphere and bulk soil zone studies, organic nitrogen components were extracted with either distilled water, 0.5 mol/L K2SO4 or acid hydrolysis. Results: K2SO4-EON constituted more than 30% of TEN pools. K2SO4-extractable AAs accounted for 25% of EON pools and nearly 10% of TEN pools in rhizosphere soils. Overall, both K2SO4-EON and extractable AAs contents had positive correlations with TEN pools. Conclusions: EON represented a major component of TEN pools in garden and paddy soils under traditional planting conditions. Although only a small proportion of the EON was present in the form of water-extractable and K2SO4-extractable AAs, the release of AAs from soil exchangeable sites might be an important source of organic nitrogen (N) for plant growth. Our findings suggest that the content of most organic forms of N was significantly greater in rhizosphere than in bulk soil zone samples. However...

Determination of the flavonoid components of cashew apple (Anacardium occidentale) by LC-DAD-ESI/MS

de Brito, Edy Sousa; de Araújo, Manuela Cristina Pessanha; Lin, Long-Ze; Harnly, James
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em //2007 EN
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Liquid chromatography, with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI/MS), was used to identify and quantify flavonoids in cashew apple. One anthocyanin and thirteen glycosylated flavonols were detected in a methanol–water extract. Among them, the 3-O-galactoside, 3-O-glucoside, 3-O-rhamnoside, 3-O-xylopyranoside, 3-O-arabinopyranoside and 3-O-arabinofuranoside of quercetin and myricetin, as well as kaempferol 3-O-glucoside were identified by direct comparison with standards or positively identified flavonoids in cranberry. The anthocyanin was the 3-O-hexoside of methyl-cyanidin. Trace amounts of delphinidin and rhamnetin were detected in the hydrolyzed extract, suggesting their glycosides were present, but undetectable, in the original extract. The concentrations of the 14 flavonoids in the tested sample were determined. This is the first report of these flavonoids in cashew apple.

Honey Bee Foraging Preferences, Effects of Sugars, and Fruit Fly Toxic Bait Components

Mangan, Robert L.; Tarshis Moreno, Aleena
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica Formato: text/html
EN
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26.08%
Field tests were carried out to evaluate the repellency of the Dow AgroSciences fruit fly toxic bait GF-120 (NF Naturalyte) to domestic honey bees (Apis mellifera L.). GF-120 is an organically registered attractive bait for tephritid fruit flies composed of spinosad, hydrolyzed protein (Solulys), high-fructose corn syrup (ADM CornSweet 42 high-fructose corn syrup, referred to as invertose sugar or invertose here), vegetable oils, adjuvants, humectants, and attractants. Tests were carried out with non-Africanized honey bees in February and March 2005 and 2007 during periods of maximum hunger for these bees. In all tests, bees were first trained to forage from plates of 30% honey-water (2005) or 30% invertose (2007). In 2005 bees were offered choices between honey-water and various bait components, including the complete toxic bait. In 2007, similar tests were performed except bees were attracted with 30% invertose then offered the bait components or complete bait as no-choice tests. Initially, the 2005 tests used all the components of GF-120 except the spinosad as the test bait. After we were convinced that bees would not collect or be contaminated by the bait, we tested the complete GF-120. Behavior of the bees indicated that during initial attraction and after switching the baits...

Quantitative measures of lambs fed hydrolyzed sugarcane under aerobic and anaerobic conditions

Endo, Viviane; Silva Sobrinho, Americo Garcia da; Lins Lima, Natalia Ludmila; Almeida, Fabiana Alves de; Brancacci Lopes Zeola, Nivea Maria
Fonte: Univ Estadual Londrina Publicador: Univ Estadual Londrina
Tipo: Artigo de Revista Científica Formato: 1055-1066
ENG
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); This study aimed to evaluate the influence of aerobic and anaerobic conditions on hydrolyzed sugarcane, over the in vivo morphological measurements, quantitative traits of carcass and non-carcass components of lambs. Twenty four Ile de France lambs, from 15 to 32 kg of body weight (BW) were used. Treatments were: in natura sugarcane (IN), sugarcane hydrolyzed using 0.6% calcium oxide (CaO) under aerobic condition (AER), and sugarcane hydrolyzed using 0.6% CaO under anaerobic condition (ANA). Treatments were supplied to animals along with concentrate. Before slaughtering, the lambs were weighed and morphological measurements were taken. After slaughtering, carcasses were evaluated for weight, dressing, non-carcass components, as well as their morphological measurements and weight loss by cooling. A completely randomized design with three treatments and eight replicates was used and the means were compared by Tukey test at 5% of significance. Lambs fed hydrolyzed sugarcane in aerobic condition had higher leg length (32.21 cm) and lower rump height (55.23 cm) in vivo, whereas empty body weight (24.84 kg) was lower for lambs fed in natura sugarcane. No difference for the other variables was observed. Sugarcane hydrolyzed under aerobic and anaerobic conditions is not an important factor affecting the in vivo...

Estimación de la exposición a micotoxinas a través de dos técnicas: ocratoxina A en plasma y biodisponibilidad de fumonisina B1 en copos de maíz; Exposition estimation to mycotoxins by two techniques: ochratoxin a in plasma and bioaccesibility of fumonisin B1 from corn flakes

Motta, Estela Leonor
Fonte: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires Publicador: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Tipo: info:eu-repo/semantics/doctoralThesis; tesis doctoral; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2009 SPA
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La presencia de ocratoxina A (OTA) en sangre humana ha sido informada en muchos países, especialmente Europa. Sin embargo, hasta el momento no existen estudios que refieran su presencia en la Argentina. El objetivo de este estudio fue evaluar la concentración de OTA en plasma humano en dos localidades de la provincia de Buenos Aires. Las determinaciones de OTA en 199 muestras de donantes de sangre en Mar del Plata y 236 de General Rodríguez fueron realizadas por HPLC. La limpieza de las muestras se realizó con una columna Bakerbond® C-18 y una purificación final con columna de inmunoafinidad Ochraprep®. El límite de cuantificación de la OTA fue de 0,019 ng/ml. La confirmación de la presencia de la OTA fue realizada a través de la formación del metil éster de la OTA. El análisis reveló que 63,8 % y 62,3 % de las muestras plasmáticas de Mar del Plata y de General Rodríguez respectivamente fueron positivas para OTA. Se determinó un promedio winsorizado de 0,15 ng/ml y 0,43 ng/ml respectivamente. La encuesta alimentaria realizada permitió observar que la principal fuente de ingesta son los cereales y panificados, utilizando valores de ocurrencia de otros países se obtuvo un valor ingesta de OTA promedio de 2,21 ng/kg p.c./día...

Solid phase microextraction as a reliable alternative to conventional extraction techniques to evaluate the pattern of hydrolytically released components in Vitis vinifera L. grapes

Perestrelo, Rosa; Caldeira, Michael; Câmara, José S.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em /06/2012 ENG
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In present research, headspace solid-phase microextraction (HS-SPME) followed by gas chromatography–mass spectrometry (GC–qMS), was evaluated as a reliable and improved alternative to the commonly used liquid–liquid extraction (LLE) technique for the establishment of the pattern of hydrolytically released components of 7 Vitis vinifera L. grape varieties, commonly used to produce the world-famous Madeira wine. Since there is no data available on their glycosidic fractions, at a first step, two hydrolyse procedures, acid and enzymatic, were carried out using Boal grapes as matrix. Several parameters susceptible of influencing the hydrolytic process were studied. The best results, expressed as GC peak area, number of identified components and reproducibility, were obtained using ProZym M with b-glucosidase activity at 35 °C for 42 h. For the extraction of hydrolytically released components, HS-SPME technique was evaluated as a reliable and improved alternative to the conventional extraction technique, LLE (ethyl acetate). HS-SPME using DVB/CAR/PDMS as coating fiber displayed an extraction capacity two fold higher than LLE (ethyl acetate). The hydrolyzed fraction was mainly characterized by the occurrence of aliphatic and aromatic alcohols...