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Resultados filtrados por Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Ciências Farmacêuticas; Ciências da Saúde - Farmácia

Desenvolvimento e validação de uma metodologia analítica, em CLAE-PDA, para avaliação da estabilidade do 4-nerolidilcatecol na presença de seus produtos de degradação; Development and validation of an HPLC-PDA method for 4-nerolidylcatechol stability-indicating assay method

CARDOSO, Fabiana Fernandes de Santana e Silva
Fonte: Universidade Federal de Goiás; BR; UFG; Mestrado em Ciências Farmacêuticas; Ciências da Saúde - Farmácia Publicador: Universidade Federal de Goiás; BR; UFG; Mestrado em Ciências Farmacêuticas; Ciências da Saúde - Farmácia
Tipo: Dissertação Formato: application/pdf
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The 4-nerolidylcatechol is the main secondary metabolite of the species Pothomorphe umbellata L. Miq. with established antioxidant, anti-inflammatory and photoprotective actions. Nevertheless, after its isolation and storage, evidence of photoinstability is observed, in a way that the characterization of the factors that influence such instability become mandatory, facing the perspective of a new drug development. Therefore, the aim of this paper was to develop and validate a hromatographic methodology able to analyze the degradation kinetic of the 4-NRC, in the presence of the degradation products, using the high performance liquid chromatography coupled to a detector with photodiodes (HPLC-PDA). Our results showed that the analytical method was selective (5 μg/mL) and linear over a wide range of concentrations (5.0 to 500.0 μg/mL) indicating high intra-day (0,02 2.83%) and inter-day precision (0.59 4.89%), besides accuracy (98.46 104.88%) and ruggedness. The photolytic study showed a fast degradation of 4- nerolidylcatechol (tR = 25 min), generating a major product after a total run time of 8 min. The hydrolytic study showed high instability of the substance in alkaline medium (pH 13.00), generating a major product after a chromatographic run time of 26 min. In acid conditions (pH 1.00)...