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Estudos genético-moleculares em Panicum maximum : mapeamento genético-molecular e análise de transcriptoma via RNA-seq = Genetic and molecular studies in Panicum maximum: genetic and molecular mapping and transcriptome analysis via RNA-seq; Genetic and molecular studies in Panicum maximum : genetic and molecular mapping and transcriptome analysis via RNA-seq

Guilherme de Toledo e Silva
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 28/02/2014 PT
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No Brasil, a pecuária bovina é baseada principalmente na utilização de pastagens cultivadas para alimentação animal. A espécie Panicum maximum Jacq., popularmente conhecida no Brasil como capim colonião, encontra-se entre as espécies mais utilizadas na alimentação do gado de corte. Grande parte das áreas destinadas a pastagens encontra-se estabelecida com cultivares exóticas e de reprodução clonal. O monocultivo representa sério risco para todos os sistemas de produção baseados no uso de pastagens. Nesse sentido, o melhoramento genético de forrageiras e o lançamento de novas cultivares de P. maximum surgem como alternativa para a diversificação das pastagens. O presente trabalho teve como objetivo principal contribuir para o conhecimento básico sobre a genética e a biologia molecular de P. maximum. Primeiramente, foi realizada a montagem de novo e análise do transcriptoma da espécie utilizando a metodologia de sequenciamento massivo paralelo de cDNA (RNA-seq), resultando em 38.192 unigenes, os quais foram anotados em diferentes bancos de dados. A maioria dos genes relacionados as vias de fixação de carbono C4 e de síntese de lignocelulose foram identificados. Ainda, foram identificados 5.035 marcadores microssatélites (SSR) e 346.456 marcadores do tipo single nucleotide polymorphism (SNP) em todo o transcriptoma. Na segunda parte deste trabalho...

Integration of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Cloning for the Identification and Functional Characterization of Mobile ortho-Halobenzoate Oxygenase Genes in Pseudomonas aeruginosa Strain JB2

Hickey, W. J.; Sabat, G.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /12/2001 EN
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Protein mass spectrometry and molecular cloning techniques were used to identify and characterize mobile o-halobenzoate oxygenase genes in Pseudomonas aeruginosa strain JB2 and Pseudomonas huttiensis strain D1. Proteins induced in strains JB2 and D1 by growth on 2-chlorobenzoate (2-CBa) were extracted from sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and analyzed by matrix-assisted laser desorption ionization–time of flight mass spectrometry. Two bands gave significant matches to OhbB and OhbA, which have been reported to be the α and β subunits, respectively, of an ortho-1,2-halobenzoate dioxygenase of P. aeruginosa strain 142 (T. V. Tsoi, E. G. Plotnikova, J. R. Cole, W. F. Guerin, M. Bagdasarian, and J. M. Tiedje, Appl. Environ. Microbiol. 65:2151–2162, 1999). PCR and Southern hybridization experiments confirmed that ohbAB were present in strain JB2 and were transferred from strain JB2 to strain D1. While the sequences of ohbA from strains JB2, D1, and 142 were identical, the sequences of ohbB from strains JB2 and D1 were identical to each other but differed slightly from that of strain 142. PCR analyses and Southern hybridization analyses indicated that ohbAB were conserved in strains JB2 and D1 and in strain 142 but that the regions adjoining these genes were divergent. Expression of ohbAB in Escherichia coli resulted in conversion of o-chlorobenzoates to the corresponding (chloro)catechols with the following apparent affinity: 2-CBa ≈ 2...

Genetics and Regulation of Chitobiose Utilization in Borrelia burgdorferi

Tilly, Kit; Elias, Abdallah F.; Errett, Jennifer; Fischer, Elizabeth; Iyer, Radha; Schwartz, Ira; Bono, James L.; Rosa, Patricia
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /10/2001 EN
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Borrelia burgdorferi spends a significant proportion of its life cycle within an ixodid tick, which has a cuticle containing chitin, a polymer of N-acetylglucosamine (GlcNAc). The B. burgdorferi celA, celB, and celC genes encode products homologous to transporters for cellobiose and chitobiose (the dimer subunit of chitin) in other bacteria, which could be useful for bacterial nutrient acquisition during growth within ticks. We found that chitobiose efficiently substituted for GlcNAc during bacterial growth in culture medium. We inactivated the celB gene, which encodes the putative membrane-spanning component of the transporter, and compared growth of the mutant in various media to that of its isogenic parent. The mutant was no longer able to utilize chitobiose, while neither the mutant nor the wild type can utilize cellobiose. We propose renaming the three genes chbA, chbB, and chbC, since they probably encode a chitobiose transporter. We also found that the chbC gene was regulated in response to growth temperature and during growth in medium lacking GlcNAc.

Cloning and Molecular Characterization of a Multicopy, Linear Plasmid-Carried, Repeat Motif-Containing Gene from Borrelia turicatae, a Causative Agent of Relapsing Fever

Carlyon, Jason A.; Marconi, Richard T.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /09/1998 EN
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Borrelia turicatae is one of several spirochete species that can cause relapsing fever. Here, we describe the identification and characterization of a gene from B. turicatae and other relapsing-fever spirochetes that exhibits homology with the rep+ and ORF-E gene families of the Lyme disease spirochetes. This gene, which we have designated repA, encodes a putative protein of 30.2 kDa with an isoelectric point of 4.69. The central region of RepA harbors a series of amino acid repeat motifs which exhibit homology with casein kinase 2 phosphorylation sites. Through Southern hybridization analyses, we demonstrate that repA (or a closely related sequence) is multicopy in the relapsing-fever spirochetes and is carried on variably sized linear plasmids in both Borrelia parkeri and B. turicatae. Transcriptional analyses demonstrate that repA is expressed, albeit at low levels, during in vitro cultivation of B. turicatae. Transcriptional start site analysis revealed that repA is preceded by a consensus ribosomal binding site and an appropriately spaced promoter element. The sequence conservation, unique features, and multicopy status of repA and its homologs suggest that RepA may play an important genus-wide role in the biology of the Borrelia.

Transfer and Incorporation of Genes Controlling β-d-Galactosidase Synthesis from Hfr and F′ Donors of Escherichia coli1

Ganesan, Ann K.; Rotman, Boris
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1966 EN
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Ganesan, Ann K. (Syntex Institute of Molecular Biology, Palo Alto, Calif.), and Boris Rotman. Transfer and incorporation of genes controlling β-d-galactosidase synthesis from Hfr and F′ donors of Escherichia coli. J. Bacteriol. 92:1378–1382. 1966.—Comparisons were made between Hfr1 and F13 donors with respect to the frequency of transfer and incorporation of genes controlling β-d-galactosidase synthesis. The Hfr1 donor transfers these genes as part of the chromosome, and the F13 donor transfers them by F-duction. The criterion used for gene transfer was the acquisition by recipient cells of the ability to synthesize the enzyme, β-d-galactosidase, measured by fluorogenic assays at the single-cell level. The criterion for incorporation was the formation of lac+ recombinant colonies. It was found that the two types of donor showed the same frequency of gene transfer, but the probability of incorporation was 10-fold higher in F13 matings than in Hfr1 matings. In the former, between 46 and 97% of the merozygotes produced recombinant colonies; in the latter, 2 to 6% did so.

Structural and Functional Characterization of Three Polyketide Synthase Gene Clusters in Bacillus amyloliquefaciens FZB 42

Chen, Xiao-Hua; Vater, Joachim; Piel, Jörn; Franke, Peter; Scholz, Romy; Schneider, Kathrin; Koumoutsi, Alexandra; Hitzeroth, Gabriele; Grammel, Nicolas; Strittmatter, Axel W.; Gottschalk, Gerhard; Süssmuth, Roderich D.; Borriss, Rainer
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /06/2006 EN
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Although bacterial polyketides are of considerable biomedical interest, the molecular biology of polyketide biosynthesis in Bacillus spp., one of the richest bacterial sources of bioactive natural products, remains largely unexplored. Here we assign for the first time complete polyketide synthase (PKS) gene clusters to Bacillus antibiotics. Three giant modular PKS systems of the trans-acyltransferase type were identified in Bacillus amyloliquefaciens FZB 42. One of them, pks1, is an ortholog of the pksX operon with a previously unknown function in the sequenced model strain Bacillus subtilis 168, while the pks2 and pks3 clusters are novel gene clusters. Cassette mutagenesis combined with advanced mass spectrometric techniques such as matrix-assisted laser desorption ionization-time of flight mass spectrometry and liquid chromatography-electrospray ionization mass spectrometry revealed that the pks1 (bae) and pks3 (dif) gene clusters encode the biosynthesis of the polyene antibiotics bacillaene and difficidin or oxydifficidin, respectively. In addition, B. subtilis OKB105 (pheA sfp0), a transformant of the B. subtilis 168 derivative JH642, was shown to produce bacillaene, demonstrating that the pksX gene cluster directs the synthesis of that polyketide.

Characterization of the Transcriptional Activity of the Cryptic Plasmid pRN1 from Sulfolobus islandicus REN1H1 and Regulation of Its Replication Operon▿ †

Berkner, Silvia; Lipps, Georg
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
EN
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75.49%
The plasmid pRN1 from Sulfolobus islandicus REN1H1 belongs to the crenarchaeal plasmid family pRN. The plasmids in this family encode three conserved proteins that participate in plasmid replication and copy number regulation, as suggested by biochemical characterization of the recombinant proteins. In order to deepen our understanding of the molecular biology of these plasmids, we investigated the transcriptional activity of the model plasmid pRN1. We detected five major transcripts present at about 2 to 15 copies per cell. One long transcriptional unit comprises the genes for the plasmid-copy-number control protein Orf56/CopG and the replication protein Orf904. A second transcript with a long 3′-untranslated region codes for the DNA binding protein Orf80. For both transcripts, we identified countertranscripts which could play a regulatory role. The function of the fifth transcript is unclear. For the five transcripts, we determined the start site, the transcript end, the stability, and the abundance in different growth phases. Reporter gene experiments demonstrated that the copy number control protein Orf56 represses transcription of the orf56-orf904 cotranscript in vivo.

Molecular Genetics and Genomic Analysis of Scytonemin Biosynthesis in Nostoc punctiforme ATCC 29133▿

Soule, Tanya; Stout, V.; Swingley, W. D.; Meeks, J. C.; Garcia-Pichel, F.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
EN
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The indole-alkaloid scytonemin is the most common and widespread sunscreen among cyanobacteria. Previous research has focused on its nature, distribution, ecology, physiology, and biochemistry, but its molecular genetics have not been explored. In this study, a scytonemin-deficient mutant of the cyanobacterium Nostoc punctiforme ATCC 29133 was obtained by random transposon insertion into open reading frame NpR1273. The absence of scytonemin under conditions of induction by UV irradiation was the single phenotypic difference detected in a comparative analysis of the wild type and the mutant. A cause-effect relationship between the phenotype and the mutation in NpR1273 was demonstrated by constructing a second scytoneminless mutant through directed mutagenesis of that gene. The genomic region flanking the mutation revealed an 18-gene cluster (NpR1276 to NpR1259). Four putative genes in the cluster, NpR1274 to NpR1271, with no previously known functions, are likely to be involved in the assembly of scytonemin. Also in this cluster, there is a redundant set of genes coding for shikimic acid and aromatic amino acid biosynthesis enzymes, leading to the production of tryptophan and tyrosine, which are likely to be biosynthetic precursors of the sunscreen.

Combination of Chemical Genetics and Phosphoproteomics for Kinase Signaling Analysis Enables Confident Identification of Cellular Downstream Targets*

Oppermann, Felix S.; Grundner-Culemann, Kathrin; Kumar, Chanchal; Gruss, Oliver J.; Jallepalli, Prasad V.; Daub, Henrik
Fonte: The American Society for Biochemistry and Molecular Biology Publicador: The American Society for Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
EN
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85.47%
Delineation of phosphorylation-based signaling networks requires reliable data about the underlying cellular kinase-substrate interactions. We report a chemical genetics and quantitative phosphoproteomics approach that encompasses cellular kinase activation in combination with comparative replicate mass spectrometry analyses of cells expressing either inhibitor-sensitive or resistant kinase variant. We applied this workflow to Plk1 (Polo-like kinase 1) in mitotic cells and induced cellular Plk1 activity by wash-out of the bulky kinase inhibitor 3-MB-PP1, which targets a mutant kinase version with an enlarged catalytic pocket while not interfering with wild-type Plk1. We quantified more than 20,000 distinct phosphorylation sites by SILAC, approximately half of which were measured in at least two independent experiments in cells expressing mutant and wild-type Plk1. Based on replicate phosphorylation site quantifications in both mutant and wild-type Plk1 cells, our chemical genetic proteomics concept enabled stringent comparative statistics by significance analysis of microarrays, which unveiled more than 350 cellular downstream targets of Plk1 validated by full concordance of both statistical and experimental data. Our data point to hitherto poorly characterized aspects in Plk1-controlled mitotic progression and provide a largely extended resource for functional studies. We anticipate the described strategies to be of general utility for systematic and confident identification of cellular protein kinase substrates.

Applying Genetics and Molecular Biology to the Study of the Human Pathogen Cryptococcus neoformans

Chun, Cheryl D.; Madhani, Hiten D.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
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85.56%
The basidiomycete yeast Crytococcus neoformans is a prominent human pathogen. It primarily infects immunocompromised individuals producing a meningoencephalitis that is lethal if untreated. Recent advances in its genetics and molecular biology have made it a model system for understanding both the Basidiomycota phylum and mechanisms of fungal pathogenesis. The relative ease of experimental manipulation coupled with the development of murine models for human disease allow for powerful studies in the mechanisms of virulence and host responses. This chapter introduces the organism and its life cycle and then provides detailed step-by-step protocols for culture, manipulation of the genome, analysis of nucleic acids and proteins, and assessment of virulence and expression of virulence factors.

Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli

Mosberg, Joshua Adam Weintrob
Fonte: Harvard University Publicador: Harvard University
Tipo: Thesis or Dissertation
EN_US
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75.59%
The phage-derived Lambda Red recombination system utilizes exogenous DNA in order to generate precise insertion, deletion, and point mutations in Escherichia coli and other bacteria. Due to its convenience, it is a frequently-used tool in genetics and molecular biology, as well as in larger-scale genome engineering projects. However, limited recombination frequency constrains the usefulness of Lambda Red for several important applications. In this work, I utilize a mechanism-guided approach in order to improve the power and utility of Lambda Red recombination.

The genetics and molecular biology of unc.-86, a C. elegans cell lineage gene

Finney, Michael
Fonte: Massachusetts Institute of Technology Publicador: Massachusetts Institute of Technology
Tipo: Tese de Doutorado Formato: [3], 174 leaves; 6918542 bytes; 6918300 bytes; application/pdf; application/pdf
ENG
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75.51%
by Michael Finney.; Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1987.; Bibliography: leaves 172-174.

Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl.

GUIMARAES, C. T.; HONEYCUTT, R.J.; SILLS, G.R.; SOBRAL, W.S.
Fonte: Genetics and Molecular Biology, Ribeirão Preto, v. 22, n. 1, p. 125-132, 1999. Publicador: Genetics and Molecular Biology, Ribeirão Preto, v. 22, n. 1, p. 125-132, 1999.
Tipo: Artigo em periódico indexado (ALICE)
PT_BR
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95.59%
Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum La Purple) and its proposed progenitor species (S. robustum "Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps to both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1.881 cM, white the Mol 5829 map contained 301 SDMs, spanning 65 linkage gropus and 1,189 cM, Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae.; 1999

The Eukaryotic Ribosome: Current Status and Challenges*

Dinman, Jonathan D.
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
Publicado em 01/05/2009 EN
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75.49%
Despite having been identified first, their greater degree of complexity has resulted in our understanding of eukaryotic ribosomes lagging behind that of their bacterial and archaeal counterparts. A much more complicated biogenesis program results in ribosomes that are structurally, biochemically, and functionally more complex. However, recent advances in molecular genetics and structural biology are helping to reveal the intricacies of the eukaryotic ribosome and to address many longstanding questions regarding its many roles in the regulation of gene expression.

Investigation of the properties of wild type and mutant alkaline phosphatase variants: a laboratory project linking genotype and phenotype

Howitt, Susan
Fonte: International Union of Biochemistry and Molecular Biology Publicador: International Union of Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
75.52%
An understanding of the link between genotype and phenotype is essential for biology students. A 3-wk laboratory project aimed at demonstrating this link and introducing early year students to some aspects of the research process is described. Students in

Graphical Modelling in Genetics and Systems Biology

Scutari, Marco
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
75.55%
Graphical modelling has a long history in statistics as a tool for the analysis of multivariate data, starting from Wright's path analysis and Gibbs' applications to statistical physics at the beginning of the last century. In its modern form, it was pioneered by Lauritzen and Wermuth and Pearl in the 1980s, and has since found applications in fields as diverse as bioinformatics, customer satisfaction surveys and weather forecasts. Genetics and systems biology are unique among these fields in the dimension of the data sets they study, which often contain several hundreds of variables and only a few tens or hundreds of observations. This raises problems in both computational complexity and the statistical significance of the resulting networks, collectively known as the "curse of dimensionality". Furthermore, the data themselves are difficult to model correctly due to the limited understanding of the underlying mechanisms. In the following, we will illustrate how such challenges affect practical graphical modelling and some possible solutions.; Comment: Workshop on Foundations of Biomedical Knowledge Representation, October 29 - November 2, 2012 Leiden, The Netherlands

Quantum Genetics and Quantum Automata Models of Quantum-Molecular Evolution Involved in the Evolution of Organisms and Species

I. C. Baianu
Fonte: Nature Preceedings Publicador: Nature Preceedings
Tipo: Manuscript
Relevância na Pesquisa
75.72%
Previous theoretical or general approaches to the problems of Quantum Genetics and Molecular Evolution are considered in this article from the point of view of Quantum Automata Theory first published by the author in 1971 and further developed in several recent articles. The representation of genomes and Interactome networks in categories of many-valued logic LMn –algebras that are naturally transformed during biological evolution, or evolve through interactions with the environment provide a new insight into the mechanisms of molecular evolution, as well as organismal evolution, in terms of sequences of quantum automata. Phenotypic changes are expressed only when certain environmentally-induced quantum-molecular changes are coupled with an internal re-structuring of major submodules of the genome and Interactome networks related to cell cycling and cell growth. Contrary to the commonly held view of `standard’ Darwinist models of evolution, the evolution of organisms and species occurs through coupled multi-molecular transformations induced not only by the environment but actually realized through internal re-organizations of genome and interactome networks. The biological, evolutionary processes involve certain epigenetic transformations that are responsible for phenotypic expression of the genome and Interactome transformations initiated at the quantum-molecular level. It can thus be said that only quantum genetics can provide correct explanations of evolutionary processes that are initiated at the quantum--multi-molecular levels and propagate to the higher levels of organismal and species evolution. Biological evolution should be therefore regarded as a multi-scale process which is initiated by underlying quantum (coupled) multi-molecular transformations of the genomic and interactomic networks...

Quantum Genetics and Quantum Automata Models of Quantum-Molecular Evolution Involved in the Evolution of Organisms and Species

I. C. Baianu
Fonte: Nature Preceedings Publicador: Nature Preceedings
Tipo: Manuscript
Relevância na Pesquisa
75.72%
Previous theoretical or general approaches to the problems of Quantum Genetics and Molecular Evolution are considered in this article from the point of view of Quantum Automata Theory first published by the author in 1971 and further developed in several recent articles. The representation of genomes and Interactome networks in categories of many-valued logic LMn –algebras that are naturally transformed during biological evolution, or evolve through interactions with the environment provide a new insight into the mechanisms of molecular evolution, as well as organismal evolution, in terms of sequences of quantum automata. Phenotypic changes are expressed only when certain environmentally-induced quantum-molecular changes are coupled with an internal re-structuring of major submodules of the genome and Interactome networks related to cell cycling and cell growth. Contrary to the commonly held view of 'standard’ Darwinist models of evolution, the evolution of organisms and species occurs through coupled multi-molecular transformations induced not only by the environment but actually realized through internal re-organizations of genome and interactome networks. The biological, evolutionary processes involve certain epigenetic transformations that are responsible for phenotypic expression of the genome and Interactome transformations initiated at the quantum-molecular level. It can thus be said that only quantum genetics can provide correct explanations of evolutionary processes that are initiated at the quantum--multi-molecular levels and propagate to the higher levels of organismal and species evolution. Biological evolution should be therefore regarded as a multi-scale process which is initiated by underlying quantum (coupled) multi-molecular transformations of the genomic and interactomic networks...

Atualização em genética da surdez não-sindrômica; Atualization in genetics of nonsyndromic hereditary deafness

Braga, Maria Cristina C.; Abreu-Silva, Ronaldo Serafim; Lezirovitz, Karina; Mingroni-Netto, Regina C.
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; Formato: application/pdf
Publicado em 03/03/2001 POR
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Nonsyndromic hereditary deafness is a highly heterogeneous condition. Many different genes from many loci have been shown to influence the development and function of hearing. Here we present a review on the genetics and molecular biology of hearing loss. There exist about 30 different autosomal locirelated to recessive hearing loss, 40 dominant loci, eight X-linked loci; five different mitochondrial mutations have been already described. 80% of hereditary nonsyndromic hearing loss is produced by recessive mechanismand one specific mutation, 35delG, in the Conexin 26 gene, is the most frequent cause of congenital neurosensorial hearing loss. An increasing number of publications report cases of inherited hearing loss due to mitochondrial mutations; the most frequent of which is known as A1555G. In many families, hearing loss is associated to the use of aminoglicosidic antibiotics. Recent advances in molecular biology of hearing loss suggest that the screening of the frequent mutations in deaf populations should be considered.; A surdez não-sindrômica é uma condição altamente heterogênea, com inúmeros genes de locos diferentes interferindo no desenvolvimento e na fisiologia da audição. Apresentamos uma revisão sobre a genética e biologia molecular do defeito. Estão envolvidos na surdez hereditária não-sindrômica cerca de 30genes autossômicos recessivos e 40 dominantes...

Information, Genetics and Entropy; Informação, Genética e Entropia

Rubio Barrios, Julio Ernesto; School of Humanities and Social Science of ITESM - Ciudad de México.
Fonte: Federal University of Santa Catarina – UFSC Publicador: Federal University of Santa Catarina – UFSC
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 30/04/2015 ENG
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http://dx.doi.org/10.5007/1808-1711.2015v19n1p121The consolidation of the informational paradigm in molecular biology research concluded on a system to convert the epistemic object into an operational technological object and a stable epistemic product. However, the acceptance of the informational properties of genetic acids failed to clarify the meaning of the concept of information. The “information”’ as a property of the genetic molecules remained as an informal notion that allows the description of the mechanism of inheritance, but it was not specified in a logic–semantic structure. The metaphorical implications associated with the idea of genes as molecules with meaning, questioned the linguistics that seemed too foreign to molecular biology. A reformulation of the concept of information in molecular biology was developed upon the theory of Claude Shannon. The node for the structural coupling between biology, physics and information theory was the identification of an analog structure between the coded messages of Shannon’s theory.; http://dx.doi.org/10.5007/1808-1711.2015v19n1p121A consolidação do paradigma informacional na pesquisa biológica molecular culminou em um sistema para converter o objeto epistêmico num objeto tecnológico-operacional e um produto epistêmico estável. Contudo...