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Relações entre seleção de testadores de milho e suas divergências genéticas; Relationship of Maize Testers Selection and their Genetic Divergences

Alves, Geovani Ferreira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 14/12/2006 PT
Relevância na Pesquisa
46.2%
Os objetivos deste trabalho foram relacionar as magnitudes das correlações entre os testecrosses com as divergências genéticas dos testadores, a fim de verificar a possibilidade da redução do número de testadores e, também, se a intensidade de seleção que pode ser aplicada é função das divergências genéticas dos testadores. Cinco testadores, previamente avaliados em um dialelo completo, foram cruzados com 50 linhagens de diferentes grupos heteróticos, em um esquema fatorial. Os 250 testecrosses foram avaliados em 13 ambientes no delineamento de látice simples 16x16; sendo que seis híbridos comerciais foram alocados nos experimentos como testemunhas. Os caracteres avaliados foram: produção de grãos corrigida para 15% de umidade (PROD), florescimento masculino (FM) e feminino (FF), altura da planta (AP) e espiga (AE), posição relativa da espiga (PRE), acamamento de plantas (ACMQ), prolificidade (PROL), comprimento de espiga (CE), diâmetro de espiga (DE), diâmetro de sabugo (DS), profundidade de grão (PROF), número de fileiras (NFIL), número de grãos por fileira (NGF) e peso de 500 grãos (P500). Os mesmos caracteres avaliados nos testecrosses também foram avaliados nos testadores em quatro ambientes no delineamento blocos completos...

Similaridade genética entre acessos de Bidens pilosa resistentes aos herbicidas inibidores da ALS; Genetic similarity among Bidens pilosa accesses resistant to ALS-inhibiting herbicides

Vidal, Ribas Antonio; Lamego, Fabiane Pinto; Resende, Luciane Vilela; Silva, Paulo Roberto da; Delatorre, Carla Andrea; Trezzi, Michelangelo Muzell
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
POR
Relevância na Pesquisa
56.19%
Herbicidas inibidores da enzima acetolactato sintase (ALS) têm sido amplamente utilizados no controle da planta daninha picão-preto (Bidens pilosa). A pressão de seleção causada pelo uso intensivo desses herbicidas tem selecionado biótipos de picão-preto resistentes. O objetivo deste trabalho foi avaliar o grau de similaridade genética entre acessos de picão-preto resistentes aos herbicidas inibidores da ALS, bem como a relação entre coeficiente de similaridade genética e distância geográfica desses acessos. Para isso, sementes de dois grupos de acessos de picão-preto, originárias de uma propriedade em Pato Branco, Paraná, resistentes aos herbicidas inibidores da ALS foram colhidas, e plântulas foram cultivadas em casa de vegetação na Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, em outubro de 2004. Por meio do marcador molecular RAPD (polimorfismo de DNA amplificado ao acaso) foi possível avaliar a similaridade genética entre os acessos de picão-preto. Na análise conjunta dos acessos, dos 20 primers utilizados, 17 apresentaram-se polimórficos, amplificando um total de 94 bandas. Houve baixa similaridade genética (38%) entre acessos de picão-preto resistentes aos herbicidas inibidores da ALS originários de uma mesma propriedade. Não foi observada relação entre distância genética e distância geográfica entre os acessos avaliados.; Acetolactate synthase (ALS)-inhibiting herbicides have been widely used to control the weed hairy beggarticks (Bidens pilosa). The selection pressure caused by intensive herbicide use has selected hairy beggarticks resistant biotypes. The objectives of this work were to evaluate the degree of genetic similarity among hairy beggarticks accesses (Bidens pilosa) resistant to ALS-inhibiting herbicides...

Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Morales, Rafael Gustavo Ferreira; Resende, Juliano Tadeu Vilela; Faria, Marcos Ventura; Andrade, Marcela Carvalho; Resende, Luciane Vilela; Delatorre, Carla Andrea; Silva, Paulo Roberto da
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
ENG
Relevância na Pesquisa
56.12%
Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers...

Study of the genetic variability and similarity among and within Arachis villosulicarpa, A-pietrarellii and A-hypogaea through isoenzyme analysis

Galgaro, L.; Valls, JFM; Lopes, C. R.
Fonte: Kluwer Academic Publ Publicador: Kluwer Academic Publ
Tipo: Artigo de Revista Científica Formato: 9-15
ENG
Relevância na Pesquisa
56.17%
Arachis villosulicarpa is a perennial species cultivated for its soft and tasty seeds by indigenous inhabitants of the Mate Grosso State, Brazil. Besides A. hypogaea, this species is considered as the only species of Arachis which represents a valuable food source for human consumption. Due to the lack of knowledge concerning the genetic diversity of A. villosulicarpa, this study was conducted to evaluate the genetic variability of the accessions from the Germplasm Collection of CENARGEN/EMBRAPA (Brasilia, DF, Brazil) and Institute Agronomico (IAC, Campinas, SP, Brazil). In addition, the genetic similarity between A. villosulicarpa, the related wild species A. pietrarellii, and the cultivated peanut A. hypogaea cv. Tatu was evaluated. From the entire sample analyzed of A. villosulicarpa, the accession from Institute Agronomico showed the highest indices of diversity for both enzymatic systems analyzed, pointing this accession as a promising source of genetic variability that must be preserved in the Germplasm Bank. A high level of genetic similarity was observed between A. pietrarellii and A. villosulicarpa, supporting previous suggestions that A. pietrarellii could be the ancestral progenitor species of A. villosulicarpa or that both species originated from a common ancestor.

Genetic variation between several species of sections Extranervosae, Caulorrhizae, Heteranthae, and Triseminatae (genus Arachis) estimated by DNA polymorphism

Galgaro, Leticia; Lopes, Catalina Romero; Gimenes, Marcos; Valls, José F.M.; Kochert, Gary
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 445-454
ENG
Relevância na Pesquisa
56.12%
Genetic variation within and among accessions of the genus Arachis representing sections Extranervosae, Caulorrhizae, Heteranthae, and Triseminatae was evaluated using RFLP and RAPD markers. RAPD markers revealed a higher level of genetic diversity than did RFLP markers, both within and among the species evaluated. Phenograms based on various band-matching algorithms revealed three major clusters of similarity among the sections evaluated. The first group included the species from section Extranervosae, the second group consisted of sections Triseminatae, Caulorrhizae, and Heteranthae, and the third group consisted of one accession of Arachis hypogaea, which had been included as a representative of section Arachis. The phenograms obtained from the RAPD and RFLP data were similar but not identical. Arachis pietrarellii, assayed only by RAPD, showed a high degree of genetic similarity with Arachis villosulicarpa. This observation supported the hypothesis that these two species are closely related. It was also shown that accession V 7786, previously considered to be Arachis sp. aff. pietrarellii, and assayed using both RFLPs and RAPDs, was possibly a new species from section Extranervosae, but very distinct from A. pietrarellii.

Genetic similarity between coriander genotypes using ISSR markers

Melo,Roberto de A; Resende,Luciane V; Menezes,Dimas; Beck,Ana Paula A; Costa,José Carlos da; Coutinho,Alisson E; Nascimento,Ana Verônica S do
Fonte: Associação Brasileira de Horticultura Publicador: Associação Brasileira de Horticultura
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 EN
Relevância na Pesquisa
46.24%
With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16), resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão...

Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Morales,Rafael Gustavo Ferreira; Resende,Juliano Tadeu Vilela; Faria,Marcos Ventura; Andrade,Marcela Carvalho; Resende,Luciane Vilela; Delatorre,Carla Andrea; Silva,Paulo Roberto da
Fonte: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" Publicador: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 EN
Relevância na Pesquisa
56.12%
Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers...

Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients

Pretto,Luana; de-Paris,Fernanda; Machado,Alice Beatriz Mombach Pinheiro; Martins,Andreza Francisco; Barth,Afonso Luís
Fonte: Brazilian Society of Infectious Diseases Publicador: Brazilian Society of Infectious Diseases
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2013 EN
Relevância na Pesquisa
55.96%
Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients. The aim of this work was to perform pulsed-field gel electrophoresis-based molecular typing of B. cenocepacia isolates to evaluate the epidemiology of this species at our hospital. A total of 28 isolates from 23 cystic fibrosis patients were analyzed. Initially, we compared isolates obtained from the same patient at different periods of time. We then compared the pulsed-field gel electrophoresis profiles of 15 IIIA isolates, and in a third analysis, evaluated the genetic profile of 8 IIIB isolates from different patients. The pulsed-field gel electrophoresis profiles of isolates from the same patient indicated that they are genetically indistinguishable. Analysis of isolates from different patients revealed the presence of multiple clonal groups. These results do not indicate cross-transmission of a unique clone of B. cenocepacia among cystic fibrosis patients, although this has been observed in some patients. Our findings highlight the importance of adequate patient follow-up at cystic fibrosis centers and adherence to management and segregation measures in cystic fibrosis patients colonized with B. cenocepacia.

Genetic similarity among soybean (Glycine max (L) Merrill) cultivars released in Brazil using AFLP markers

Bonato,Ana Lídia V.; Calvo,Eberson S.; Geraldi,Isaias O.; Arias,Carlos Alberto A.
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2006 EN
Relevância na Pesquisa
66.27%
Genetic similarity among soybean genotypes was studied by applying the amplified fragment length polymorphism (AFLP) technique to 317 soybean cultivars released in Brazil from 1962 to 1998. Genetic similarity (GS) coefficients were estimated using the coefficient of Nei and Li (Nei and Li 1979), and the cultivars were clustered using the unweighted pair-group method with averages (UPGMA). The parentage coefficients of 100 cultivars released between 1984 and 1998 were calculated and correlated with the genetic similarity obtained by the markers. The genetic similarity coefficients varied from 0.17 to 0.97 (x = 0.61), with 56.8% of the coefficients being above 0.60 and only 9.7% equal to or less than 0.50. The similarity coefficients have remained constant during the last three decades. Dendrogram interpretation was hindered by the large number of cultivars used, but it was possible to detect groups of cultivars formed as expected from their genealogy. Another dendrogram, composed of 63 cultivars, allowed a better interpretation of the groups. Parentage coefficients among the 100 cultivars varied from zero to one (x = 0.21). However, no significant correlation (r = 0.12) was detected among the parentage coefficients and the AFLP genetic similarity. The results show the efficiency of AFLP markers in large scale studies of genetic similarity and are discussed in relation to soybean breeding in Brazil.

Change in genetic size of small-closed populations: lessons from a domestic mammal population

Ghafouri-Kesbi,Farhad
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2010 EN
Relevância na Pesquisa
56.08%
The aim of this study was to monitor changes in genetic size of a small-closed population of Iranian Zandi sheep, by using pedigree information from animals born between 1991 and 2005. The genetic size was assessed by using measures based on the probability of identity-by-descend of genes (coancestry, f, and effective population size, Ne), as well as measures based on probability of gene origin (effective number of founders, f e, effective number of founder genomes, f g, and effective number of non-founder genomes, f ne). Average coancestry, or the degree of genetic similarity of individuals, increased from 0.81% to 1.44% during the period 1993 to 2005, at the same time that Ne decreased from 263 to 93. The observed trend for f e was irregular throughout the experiment in a way that f e was 68, 87, 77, 92, and 80 in 1993, 1996, 1999, 2002, and 2005, respectively. Simultaneously, f g, the most informative effective number, decreased from 61 to 35. The index of genetic diversity (GD) which was obtained from estimates of f g,decreased about 2% throughout the period studied. In addition, a noticeable reduction was observed in the estimates of f ne from 595 in 1993 to 61 in 2005. The higher than 1 ratio of f e to f g indicated the presence of bottlenecks and genetic drift in the development of this population of Zandi sheep. From 1993 to 1999...

Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers

Oliveira,Luciana do Valle Rego; Faria,Ricardo Tadeu de; Ruas,Claudete de Fátima; Ruas,Paulo Maurício; Santos,Melissa de Oliveira; Carvalho,Valdemar P.
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2010 EN
Relevância na Pesquisa
56.1%
In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to establish a germplasm core collection for the genus and to provide support for breeding programs.

Genetic similarity among Agistemus pallinii Matioli et al (Acari: Stigmaeidae) found in citrus orchards in Viçosa, Minas Gerais State, Brazil

Matioli,André L; Pallini,Angelo; Tavares,Mara G
Fonte: Sociedade Entomológica do Brasil Publicador: Sociedade Entomológica do Brasil
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2009 EN
Relevância na Pesquisa
56.17%
Stigmaeidae are very important predators of mite and insect pests on several crops in Brazil. It is considered the second most important family of predatory mites in citrus orchards in Brazil. However, their identification, especially that of the members of the genus Agistemus, is rather difficult based only on morphology. Hence, this study describes the use of random amplified polymorphic DNA (RAPD-PCR) markers to determine the genetic similarity of an Agistemus pallinii Matioli et al population found in 2004 in a citrus orchard in Viçosa, Minas Gerais State, Brazil, preying on Panonychus citri (McGregor). Amplifi cations were performed with 12 random primers (OPAA8, OPAA19, OPAB1, OPAB5, OPAB18, OPAC9, OPAC17, OPAC19, OPAD10, OPAE9, OPAE12 and OPAE17), which generated 119 bands, with 53.8% polymorphism. The coefficients of genetic similarity among the individuals ranged from 0.68 to 0.99, indicating a high genetic similarity among them. The 3D projection analysis clustered the majority of individuals confirming their high similarity. Though individuals of A. pallinii are minute (± 360 µm long), the PCR-RAPD technique can still be used for their identification, complementing morphological analyses or for comparison of populations collected in different geographic regions. This is the first molecular study carried out with stigmaeid mites.

Genetic base of paddy rice cultivars of Southern Brazil

Raimondi,Juliana Vieira; Marschalek,Rubens; Nodari,Rubens Onofre
Fonte: Crop Breeding and Applied Biotechnology Publicador: Crop Breeding and Applied Biotechnology
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2014 EN
Relevância na Pesquisa
56.13%
The genetic knowledge of the germplasm is crucial to the plant breeding in order todevelop new varieties. This study was carried out to evaluate the genetic similarity of 40 rice cultivarsdeveloped in Southern Brazil with the use of two tolls: genealogy and AFLP markers. The genealogy approach showed that 90% of the cultivars were developed from only six parents, revealing a narrow genetic basis among these cultivars.A little genetic diversity among them was confirmed by AFLP markers, since 111polymorphic markers identified 87% similarity among them. The geneticsimilarity among the cultivars released by Epagri in Santa Catarina state waslower, compared to that among the cultivars released by others public institutions, Embrapa and Irga. This study has demonstrated that rice cultivars developed in Southern Brazil have narrow genetic base, which suggests high genetic vulnerability risk.

Genomic compatibility occurs over a wide range of parental genetic similarity in an outcrossing plant

Willi, Yvonne; Van Buskirk, Josh
Fonte: The Royal Society Publicador: The Royal Society
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.24%
The theory of inbreeding and outbreeding suggests that there is a hump-shaped relationship between the genetic similarity of sexually reproducing parents and the performance of their offspring. Inbreeding depression occurs when genetic similarity is high, whereas hybrid breakdown is expected when genetic similarity is low. Between these extremes, the effect of genetic similarity on fitness is unclear. We studied the shape of this relationship by crossing 65 target genotypes of the clonal, self-incompatible Ranunculus reptans with partner genotypes spanning a broad scale of genetic similarity, ranging from crosses within populations to between-population crosses and hybridisation with a closely related species. Offspring were raised in outdoor tubs. Results revealed a quadratic relationship between parental genetic distance and offspring performance, with the clonal component of fitness more strongly hump-shaped than the sexual component. Optimal genetic similarity encompassed a broad range of within-population and between-population crosses. This pattern of genomic compatibility has important implications for the evolution of mating systems and mate choice.

Genetic similarity and hatching success in birds.

Spottiswoode, Claire; Møller, Anders Pape
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 07/02/2004 EN
Relevância na Pesquisa
46.21%
The ecological correlates of fitness costs of genetic similarity in free-living, large populations of organisms are poorly understood. Using a dataset of genetic similarity as reflected by band-sharing coefficients of minisatellites, we show that bird species with higher genetic similarity experience elevated hatching failure of eggs, increasing by a factor of six across 99 species. Island distributions and cooperative breeding systems in particular were associated with elevated genetic similarity. These findings provide comparative evidence of detrimental fitness consequences of high genetic similarity across a wide range of species, and help to identify ecological factors potentially associated with increased risk of extinction.

Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD markers

Rego Oliveira, L.; de Faria, R.; de Fatima Ruas, C.; Ruas, P.; de Oliveira Santos Garcia, M.; Carvalho, V.
Fonte: Instituto de Tecnologia do Parana Publicador: Instituto de Tecnologia do Parana
Tipo: Artigo de Revista Científica
Publicado em //2010 EN
Relevância na Pesquisa
56.1%
In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to establish a germplasm core collection for the genus and to provide support for breeding programs.; Luciana do Valle Rego Oliveira, Ricardo Tadeu de Faria, Claudete de Fátima Ruas, Paulo Maurício Ruas, Melissa de Oliveira Santos and Valdemar P. Carvalho

Genetic similarity of citrus fresh fruit market cultivars

Oliveira,Roberto Pedroso de; Radmann,Elizete Beatriz
Fonte: Sociedade Brasileira de Fruticultura Publicador: Sociedade Brasileira de Fruticultura
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2005 EN
Relevância na Pesquisa
66.17%
The objective of this work was to assess the genetic similarity of the following citrus fresh fruit market seedless cultivars: Lane Late, Navelate, Navelina and Salustiana sweet oranges (Citrus sinensis (L.) Osbeck), Clemenules and Marisol mandarins (C. reticulata Blanco) and Okitsu satsuma mandarin (C. unshiu Marcovitch), and the hybrids Nova [C. clementina x (C. paradisi x C. tangerina)] and Ortanique (tangor probably derived from C. sinensis (L.) Osbeck x C. reticulata Blanco), utilizing isoenzymatic markers. Electrophoresis analysis of proteins extracted from leaf tissues was utilized to detect polymorphisms at ten isoenzymatic systems. Out of 30 alleles, 16 were polymorphic. The Jaccard coefficient was utilized to estimate the genetic similarity between the cultivars and the unweigthed pair-group method using an arithmetic average (UPGMA) was used to obtain the phenogram (NTSYS 1.7). The cultivars showed high genetic similarity (>72.5%), and were classified in five main groups: sweet oranges, 'Clemenules' and 'Marisol' mandarins, 'Nova', 'Ortanique', and 'Okitsu' satsuma mandarin.

Clone size distributions in networks of genetic similarity

Hernández-García, Emilio; Rozenfeld, Alejandro F.; Eguíluz, Víctor M.; Arnaud-Haond, Sophie; Duarte, Carlos M.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 2373 bytes; 421648 bytes; text/plain; application/pdf
ENG
Relevância na Pesquisa
66.07%
8 pages.-- ArXiv pre-print: http://arxiv.org/abs/q-bio/0608018.-- Final full-text version of the paper available at: http://dx.doi.org/10.1016/j.physd.2006.09.015.; We build networks of genetic similarity in which the nodes are organisms sampled from biological populations. The procedure is illustrated by constructing networks from genetic data of a marine clonal plant. An important feature in the networks is the presence of clone subgraphs, i.e. sets of organisms with identical genotype forming clones. As a first step to understand the dynamics that has shaped these networks, we point up a relationship between a particular degree distribution and the clone size distribution in the populations. We construct a dynamical model for the population dynamics, focussing on the dynamics of the clones, and solve it for the required distributions. Scale free and exponentially decaying forms are obtained depending on parameter values, being the first type obtained when clonal growth is the dominant process. Average distributions are dominated by the power law behavior presented by the fastest replicating populations.; This research was funded by a project of the BBVA Foundation (Spain), by project NETWORK (POCI/MAR/57342/2004) of the Portuguese Science Foundation (FCT) and by the project CONOCE2 (FIS2004-00953) of the Spanish MEC. S.A.H. was supported by a postdoctoral fellowship from FCT and the European Social Fund and A.F.R. by a post-doctoral fellowship from the Spanish Ministry of Education and Science.

Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Morales, Rafael Gustavo Ferreira; Resende, Juliano Tadeu Vilela; Faria, Marcos Ventura; Andrade, Marcela Carvalho; Resende, Luciane Vilela; Delatorre, Carla Andrea; Silva, Paulo Roberto da
Fonte: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz Publicador: Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/12/2011 ENG
Relevância na Pesquisa
56.12%
Most strawberry (Fragaria × ananassa Duchesne) cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers...

Genetic variability within mexican race avocado (Persea americana Mill.) germplasm collections determined by ISSRs

Cuiris-Pérez,H.; Guillén-Andrade,H.; Pedraza-Santos,M. E.; López-Medina,J.; Vidales-Fernández,I.
Fonte: Universidad Autónoma Chapingo Publicador: Universidad Autónoma Chapingo
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2009 EN
Relevância na Pesquisa
56.1%
The present study was undertaken in order to asses the existing genetic diversity within a germplasm collection of avocado (Persea americana Mill.) kept at the Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) Campo Experimental Uruapan (CEFAP-Uruapan). The parental relationship among 77 accessions (231 plants) of the Mexican race was investigated by using seven Inter Simple Sequence Repeat microsatellites (ISSRs). A total of 154 loci were detected. Percentage polymorphism ranged from 82.3 to 95.4, with number of bands ranging from 17 to 25 within accessions. Genetic similarity analysis revealed formation of two major groups, one with eleven subgroups and the other one with three subgroups. Genetic similarity was highest between accession 237 (Atlixco, Puebla) and accession XTC01 (Uruapan, Michoacan), while accessions 532 (Atlixco, Puebla) and 369 (Chilchota, Michoacan) were the most dissimilar ones. None of the accessions were found to be duplicates. In general, the present study demonstrated the usefulness of ISSRs analysis for determination of genetic diversity in avocado.