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PRACTICAL USE OF MOLECULAR MARKERS IN BEEF AND DAIRY ZEBU CATTLE IN BRAZIL

FERRAZ, Jose Bento Sterman; ELER, Joanir Pereira; MEIRELLES, Flavio Vieira; BALIEIRO, Julio Cesar Carvalho
Fonte: UNIV FED RIO GRANDE DO SUL Publicador: UNIV FED RIO GRANDE DO SUL
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
55.95%
Selection of zebu (Bos indicus) beef and dairy cattle in Brazil and the validation process of genetic markers for growth, carcass and meat quality traits and also for milk production, fat and protein milk content are discussed as concerned to the concepts and details of their use as auxiliary tools in selection processes. It is highlighted, also, the importance of right selection of ova donor cows for production of embryos to be transferred.

Listeria monocytogenes em camarão (Penaeus brasiliensis): marcadores sorológicos e genéticos no monitoramento de sua disseminação em uma unidade processadora de pescado; Listeria monocytogenes in shrimp (Penaeus brasiliensis): serologic and genetic markers to trace the dissemination in a sea food processing plant

Destro, Maria Teresa
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 25/07/1995 PT
Relevância na Pesquisa
55.97%
A ocorrência de Listeria monocytogenes em alimentos vem sendo estudada desde o início dos anos 80, após seu envolvimento em vários surtos de doença de origem alimentar. Os frutos do mar são o grupo de alimentos que despertou menor atenção por parte dos pesquisadores, apesar de terem sido envolvidos em casos esporádicos de listeriose e mesmo em surtos da doença. A amostragem ambiental e de produto, ao longo de uma linha de processamento é uma forma de localizar áreas relacionadas à contaminação do alimento permitindo que sejam feitas correções para evitar a produção de bens que exponham o consumidor a doenças. Com a finalidade de avaliar a contribuição da matéria prima e fatores ambientais na ocorrência e distribuição de L. monocytogenes em uma indústria processadora de pescados, e mais especificamente, numa linha processadora de camarão rosa (penoeus brasiliensis), é que desenvolveu-se a presente pesquisa. Também buscou-se determinar as diversidades antigênica e genética das cepas de L. monocytogenes isoladas, e correlacionar esta diversidade à sua distribuição na indústria. Assim, um total de 363 amostras coletadas em diferentes pontos de uma linha de processamento de camarão rosa foram examinadas para a presença de L. monocytogenes...

Estudo do polimorfismo genético C242T no gene da p22phox e a incidência de eventos cardiovasculares na doença arterial coronária; Identification of genetic markers with diagnosis and prognosis potential in coronary artery disease

Pereira, Alexandre da Costa
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 09/05/2008 PT
Relevância na Pesquisa
56.01%
O desenvolvimento de uma abordagem de estratificação de risco para a doença arterial coronária é certamente uma ferramenta de grande utilidade para o cardiologista clínico ou clínico geral, assim como para o planejamento de saúde pública e organização de ações de saúde pública mais eficazes. No entanto, esse conhecimento deve ser discutido dentro de um cenário de custo-efetividade e de acordo com seu potencial como objeto de valor econômico. O presente estudo tem como objetivo a identificação de fatores de risco genético de eventos cardiovasculares na população brasileira e o desenvolvimento de um algoritmo preditor que utilize essas informações para o diagnóstico. Esse trabalho encontra-se expositivamente dividido em dois módulos. No primeiro, procuramos exemplificar através de um estudo de associação genética nossa capacidade atual de encontrar e caracterizar variantes genéticas com poder de estratificação populacional com relação ao risco cardiovascular. Utilizamos dados obtidos a partir de pacientes com doença coronária multiarterial, analisando a relevância do polimorfismo C242T do gene da p22phox, subunidade protéica da NADPH oxidase, na predição de mortalidade desta população. Nossos dados permitem não apenas associar esse marcador genético a um risco aumentado de mortalidade nessa população...

Prospecção da influência de marcadores genéticos sobre características de crescimento, carcaça e qualidade de carne em bovinos da raça Nelore; Prospection of the genetic markers influence on growth, carcass and meat quality traits in Nellore cattle

Rezende, Fernanda Marcondes de
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 27/03/2009 PT
Relevância na Pesquisa
56.06%
Dados de desenvolvimento ponderal de 3.844 bovinos da raça Nelore, criados em pastagens em duas fazendas do sudoeste do Brasil, dos quais 1.889 tiveram suas carcaças avaliadas por ultra-sonografia e 674 foram confinados por 90 a 120 dias e abatidos por volta dos 24 meses de idade tiveram análises de associação com dezenas de marcadores genéticos realizadas, visando detectar a associação desses marcadores com características economicamente relevantes. Foram analisadas as características de crescimento, peso ao nascer (PNAS), peso a desmama (PDES), peso ao sobreano (PSOB), ganho de peso pós-desmama (GP345), escores visuais de conformação frigorífica (CONF), precocidade de acabamento (PREC), musculosidade (MUSC) e de carcaça área de olho de lombo medida por ultra-sonografia (AOL_US), espessuras de gorduras medida por ultra-som na região lombar (EGS_US) e na picanha (EGP8). Adicionalmente, foram analisadas as características medidas post-mortem, relacionadas a qualidade de carcaça, peso de carcaça quente (PCQ), área de olho de lombo (AOL), espessura de gordura no músculo Longissimus dorsi (EGS) e as características ligadas à qualidade de carne, perdas por exsudação após 7, 14 ou 21 dias de maturação da carne (PEX7...

Impacto de marcadores genéticos no fenótipo de rigidez arterial em uma população geral; Impact of genetic markers on arterial stiffness phenotype in a general population

Alvim, Rafael de Oliveira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 07/08/2012 PT
Relevância na Pesquisa
55.97%
Introdução: A rigidez arterial é um fenômeno complexo caracterizado pela diminuição da complacência vascular frente aos estímulos fisiológicos e patológicos. Semelhantemente a outros fenótipos cardiovasculares, a etiologia da rigidez arterial é modulada por fatores ambientais e genéticos. Levando em consideração a moderada herdabilidade e a característica poligênica do presente fenótipo, torna-se interessante a investigação de marcadores genéticos referentes aos diferentes sistemas envolvidos no remodelamento vascular. Objetivo: Avaliar o impacto dos polimorfismos C242T da subunidade p22phox da NADPH oxidase, G1036C da TXNIP, C609T/T471C da APOE, G1355A da elastina, I/D da ECA e A855G da MMP-9 no fenótipo de rigidez arterial em uma população geral. Métodos: Participaram do estudo 1.663 indivíduos da população geral da cidade de Vitória-ES. O DNA foi extraído a partir de uma amostra de sangue venoso. Posteriormente foram realizadas as genotipagens para as variantes genéticas supracitadas. A rigidez arterial foi avaliada por meio do método da velocidade de onda de pulso (VOP). Resultados: Em relação à VOP, os polimorfismos C242T da subunidade p22phox da NADPH oxidase e G1036C da TXNIP foram signifcativamente associados. Os indivíduos portadores do genótipo TT do polimorfismo C242T da subunidade p22phox (CC+TC=9...

Taenia solium and Taenia saginata: Identification of sequence characterized amplified region (SCAR) markers

Dias, Ana Karina Kerche; Aoki, Sérgio Moraes; Garcia, José Fernando; Nunes, Cáris Maroni
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 9-12
ENG
Relevância na Pesquisa
56.06%
Cysticercosis is one of the most important zoonosis, not only because of the effects on animal health and its economic consequences, but also due to the serious danger it poses to humans. The two main parasites involved in the taeniasis-cysticercosis complex in Brazil are Taenia saginata and Taenia solium. Differentiating between these two parasites is important both for disease control and for epidemiological studies. The purpose of this work was to identify genetic markers that could be used to differentiate these parasites. Out of 120 oligonucleotide decamers tested in random amplified polymorphic DNA (RAPD) assays, 107 were shown to discriminate between the two species of Taenia. Twenty-one DNA fragments that were specific for each species of Taenia were chosen for DNA cloning and sequencing. Seven RAPD markers were converted into sequence characterized amplified region (SCAR) markers with two specific for T. saginata and five specific for T. solium as shown by agarose gel electrophoresis. These markers were developed as potential tools to differentiate T. solium from T. saginata in epidemiological studies. © 2007 Elsevier Inc. All rights reserved.

Distinct genetic structure in populations of Chrysoperla externa (Hagen) (Neuroptera, Chrysopidae) shown by genetic markers ISSR and COI gene

Barbosa, Nara C. C. P.; Freitas, Sérgio De; Morales, Adriana C.
Fonte: Sociedade Brasileira de Entomologia Publicador: Sociedade Brasileira de Entomologia
Tipo: Artigo de Revista Científica Formato: 203-211
ENG
Relevância na Pesquisa
56.16%
Distinct genetic structure in populations of Chrysoperla externa (Hagen) (Neuroptera, Chrysopidae) shown by genetic markers ISSR and COI gene. Green lacewings are generalist predators, and the species Chrysoperla externa presents a great potential for use in biological control of agricultural pests due to its high predation and reproduction capacities, as well as its easy mass rearing in the laboratory. The adaptive success of a species is related to genetic variability, so that population genetic studies are extremely important in order to maximize success of the biological control. Thus, the present study used nuclear (Inter Simple Sequence Repeat - ISSR) and mitochondrial (Cytochrome Oxidase I - COI) molecular markers to estimate the genetic variability of 12 populations in the São Paulo State, Brazil, as well as the genetic relationships between populations. High levels of genetic diversity were observed for both markers, and the highest values of genetic diversity appear associated with municipalities that have the greatest areas of native vegetation. There was high haplotype sharing, and there was no correlation between the markers and the geographic distribution of the populations. The AMOVA indicated absence of genetic structure for the COI gene...

The association of genetic markers and malaria infection in the Brazilian Western Amazonian region

Beiguelman,B; Alves,FP; Moura,MM; Engracia,V; Nunes,ACS; Heckmann,MIO; Ferreira,RGM; Pereira da Silva,LH; Camargo,EP; Krieger,H
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2003 EN
Relevância na Pesquisa
55.9%
Almost all individuals (182) belonging to an Amazonian riverine population (Portuchuelo, RO, Brazil) were investigated for ascertaining data on epidemiological aspects of malaria. Thirteen genetic blood polymorphisms were investigated (ABO, MNSs, Rh, Kell, and Duffy systems, haptoglobins, hemoglobins, and the enzymes glucose-6-phosphate dehydrogenase, glyoxalase, phosphoglucomutase, carbonic anhydrase, red cell acid phosphatase, and esterase D). The results indicated that the Duffy system is associated with susceptibility to malaria, as observed in other endemic areas. Moreover, suggestions also arose indicating that the EsD and Rh loci may be significantly associated with resistance to malaria. If statistical type II errors and sample stratification could be ruled out, hypotheses on the existence of a causal mechanism or an unknown closely linked locus involved in susceptibility to malaria infection may explain the present findings.

Distinct genetic structure in populations of Chrysoperla externa (Hagen) (Neuroptera, Chrysopidae) shown by genetic markers ISSR and COI gene

Barbosa,Nara C. C. P.; Freitas,Sérgio de; Morales,Adriana C.
Fonte: Sociedade Brasileira De Entomologia Publicador: Sociedade Brasileira De Entomologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 EN
Relevância na Pesquisa
56.16%
Distinct genetic structure in populations of Chrysoperla externa (Hagen) (Neuroptera, Chrysopidae) shown by genetic markers ISSR and COI gene. Green lacewings are generalist predators, and the species Chrysoperla externa presents a great potential for use in biological control of agricultural pests due to its high predation and reproduction capacities, as well as its easy mass rearing in the laboratory. The adaptive success of a species is related to genetic variability, so that population genetic studies are extremely important in order to maximize success of the biological control. Thus, the present study used nuclear (Inter Simple Sequence Repeat - ISSR) and mitochondrial (Cytochrome Oxidase I - COI) molecular markers to estimate the genetic variability of 12 populations in the São Paulo State, Brazil, as well as the genetic relationships between populations. High levels of genetic diversity were observed for both markers, and the highest values of genetic diversity appear associated with municipalities that have the greatest areas of native vegetation. There was high haplotype sharing, and there was no correlation between the markers and the geographic distribution of the populations. The AMOVA indicated absence of genetic structure for the COI gene...

Development and characterisation of oil palm (Elaeis guineensis Jacq.) microsatellite markers.

BILLOTE, N.; RISTERUCCI, A. M.; BARCELOS, E.; NOYER, J. L.; AMBLARD, P.; BAURENS, F. C.
Fonte: In: INTERNATIONAL SIMPOSIUM ON OIL PALM GENETIC RESOURCES AND UTILIZATION, 2000, Kuala Lumpur. Proceedings... Kuala Lumpur: MPOB, 2000. Publicador: In: INTERNATIONAL SIMPOSIUM ON OIL PALM GENETIC RESOURCES AND UTILIZATION, 2000, Kuala Lumpur. Proceedings... Kuala Lumpur: MPOB, 2000.
Tipo: Artigo em anais de congresso (ALICE) Formato: p. W1-W17.
EN
Relevância na Pesquisa
56.09%
A set 21 SSR (simple sequence repeats) loci are evaluated with estimates of allele size range and expected heterozygosity in oil palm (Elaeis guineensis Jacq.) as in the close related caiaue (E. oleifera, Cortes) species where an optimal transportability of the SSR markers was observed. SSR mendelian segregation allows oil palm breeders to use SSR markers for genetic mapping and for the search of agronomic genes based on both intra- or inter-specific progenies. Multivariate data analysis show the ability of our developed SSR markers to reveal the genetic diversity structure of oil palm and caiaue natural populations, in accordance with already measured relationships and with geographica orgins. Very high levels of allelic variability indicate that SSRs will be a powerful tool for variety identification in the Elaeis species.; 2000

Construction of a microsatellite based genetic linkage map of almond.

Tavassolian, Iraj
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2008
Relevância na Pesquisa
46.22%
Almond (Prunus dulcis) is the most important nut crop in terms of world production. Due to its health benefit and high nutritional value the consumption and world supply of almond is increasing. To remain competitive in the world market, the Australian almond breeding program was established to produce cultivars with better adaptation to Australian conditions. As part of this program an almond mapping population consisting of 93 F₁ progeny derived from a cross between the American cultivar ‘Nonpareil’ (NP) and the European self-compatible cultivar ‘Lauranne’ (LA) was produced to construct the genetic linkage maps. The first almond linkage map developed prior to the commencement of this project failed to produce the eight linkage groups similar to the basic chromosome number of almond (x = 8) and many large gaps were also observed on the linkage groups. Therefore, more markers were needed to saturate the maps. Microsatellite markers are considered one of the best choices for mapping studies. 195 microsatellite markers isolated from Prunus species were obtained from published papers or by personal communication. Polymorphism was revealed by three different methods, and in general, polyacrylamide gel electrophoresis (PAGE) compared to the fluorescent labelled marker detection using an automated DNA sequencer or agarose gel electrophoresis...

Identification and characterization of genetic markers and metabolic pathways controlling net feed efficiency in beef cattle.

Naik, Madan Bhaskar
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2008
Relevância na Pesquisa
66.01%
Net feed intake or residual feed intake is the feed intake of an animal after adjustment for its average weight and weight gain while on the feed test. High net feed efficiency (NFE) animals have a low net feed intake, so the aim is to select animals that have high net feed efficiency in order to reduce the 70% expenditure for feed costs. Thus far, very few studies have been undertaken on beef cattle to identify genetic markers for NFE and to understand the molecular genetics of feed intake regulation and energy balance. Therefore, in an attempt to identify genes and metabolic pathways controlling feed efficiency in beef cattle, three different experimental approaches were taken herein: a) linkage and linkage disequilibrium quantitative trait loci (QTL) mapping for net feed intake in Lirnousin x Jersey animals, b) mitochondrial oxidative phosphorylation enzyme assays in high and low NFE cattle, and c) 2-dimensional fluorescent gel electrophoresis (DIGE) proteomics analysis of mitochondrial proteins. For the cattle QTL mapping, the results from a previous trial were utilized. In the trial, a double back-cross design was employed using two extremely divergent Bos taurus breeds [Jersey (J) dairy breed and Limousin (L) beef breed]. These breeds known to differ in many traits including carcass composition...

Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers

Kaur, S.; Pembleton, L.; Cogan, N.; Savin, K.; Leonforte, A.; Paull, J.; Materne, M.; Forster, J.
Fonte: BioMed Central Ltd. Publicador: BioMed Central Ltd.
Tipo: Artigo de Revista Científica
Publicado em //2012 EN
Relevância na Pesquisa
56.02%
BACKGROUND: Field pea (Pisum sativum L.) and faba bean (Vicia faba L.) are cool-season grain legume species that provide rich sources of food for humans and fodder for livestock. To date, both species have been relative ‘genomic orphans’ due to limited availability of genetic and genomic information. A significant enrichment of genomic resources is consequently required in order to understand the genetic architecture of important agronomic traits, and to support germplasm enhancement, genetic diversity, population structure and demographic studies. RESULTS: cDNA samples obtained from various tissue types of specific field pea and faba bean genotypes were sequenced using 454 Roche GS FLX Titanium technology. A total of 720,324 and 304,680 reads for field pea and faba bean, respectively, were de novo assembled to generate sets of 70,682 and 60,440 unigenes. Consensus sequences were compared against the genome of the model legume species Medicago truncatula Gaertn., as well as that of the more distantly related, but better-characterised genome of Arabidopsis thaliana L.. In comparison to M. truncatula coding sequences, 11,737 and 10,179 unique hits were obtained from field pea and faba bean. Totals of 22,057 field pea and 18,052 faba bean unigenes were subsequently annotated from GenBank. Comparison to the genome of soybean (Glycine max L.) resulted in 19...

Convergent evolution in the genetic basis of Müllerian mimicry in Heliconius butterflies; Convergent evolution in the genetic basis of Mullerian mimicry in Heliconius butterflies

Baxter, S.; Papa, R.; Chamberlain, N.; Humphray, S.; Joron, M.; Morrison, C.; ffrench-Constant, R.; McMillan, W.; Jiggins, C.
Fonte: Genetics Publicador: Genetics
Tipo: Artigo de Revista Científica
Publicado em //2008 EN
Relevância na Pesquisa
56.08%
The neotropical butterflies Heliconius melpomene and H. erato are Müllerian mimics that display the same warningly colored wing patterns in local populations, yet pattern diversity between geographic regions. Linkage mapping has previously shown convergent red wing phenotypes in these species are controlled by loci on homologous chromosomes. Here, AFLP bulk segregant analysis using H. melpomene crosses identified genetic markers tightly linked to two red wing-patterning loci. These markers were used to screen a H. melpomene BAC library and a tile path was assembled spanning one locus completely and part of the second. Concurrently, a similar strategy was used to identify a BAC clone tightly linked to the locus controlling the mimetic red wing phenotypes in H. erato. A methionine rich storage protein (MRSP) gene was identified within this BAC clone, and comparative genetic mapping shows red wing color loci are in homologous regions of the genome of H. erato and H. melpomene. Subtle differences in these convergent phenotypes imply they evolved independently using somewhat different developmental routes, but are nonetheless regulated by the same switch locus. Genetic mapping of MRSP in a third related species, the "tiger" patterned H. numata...

Bayesian methods for meta-analysis of causal relationships estimated using genetic instrumental variables

Thompson, S.; Burgess, S.; CRP CHD Genetics Collaboration
Fonte: John Wiley & Sons Ltd Publicador: John Wiley & Sons Ltd
Tipo: Artigo de Revista Científica
Publicado em //2010 EN
Relevância na Pesquisa
56.13%
Genetic markers can be used as instrumental variables, in an analogous way to randomization in a clinical trial, to estimate the causal relationship between a phenotype and an outcome variable. Our purpose is to extend the existing methods for such Mendelian randomization studies to the context of multiple genetic markers measured in multiple studies, based on the analysis of individual participant data. First, for a single genetic marker in one study, we show that the usual ratio of coefficients approach can be reformulated as a regression with heterogeneous error in the explanatory variable. This can be implemented using a Bayesian approach, which is next extended to include multiple genetic markers. We then propose a hierarchical model for undertaking a meta-analysis of multiple studies, in which it is not necessary that the same genetic markers are measured in each study. This provides an overall estimate of the causal relationship between the phenotype and the outcome, and an assessment of its heterogeneity across studies. As an example, we estimate the causal relationship of blood concentrations of C-reactive protein on fibrinogen levels using data from 11 studies. These methods provide a flexible framework for efficient estimation of causal relationships derived from multiple studies. Issues discussed include weak instrument bias...

Coeffcients of herdability and relatedness in a forest fragment of Araucaria angustifolia (Bertol.) Kuntze using genetic markers

Bueno da Silva, Erica Cristina; Kuboyama Kubota, Thaisa Yuriko; Teixeira de Moraes, Mario Luiz; Sebbenn, Alexandre Magno
Fonte: Ipef-inst Pesquisas Estudos Florestais Publicador: Ipef-inst Pesquisas Estudos Florestais
Tipo: Artigo de Revista Científica Formato: 147-153
POR
Relevância na Pesquisa
55.95%
Methods based on genetic markers to estimate the coefficient of heritability in natural populations are important to understand the effects of natural selection on inheritance of quantitative traits. The objective of this study was to investigate the genetic control of the trait plant height in a fragmented population of Araucaria angustifolia. This study was conducted in a forest fragment of 5.4 ha of area, located in the State of Parana, Brazil. Estimates of heritability were performed using data from genotypes and height of regenerating individuals of the population. Four methods to estimate the relatedness between pairs of individuals (RITLAND, 1996; LYNCH; RITLAND, 1999; QUELLER; GOODNIGHT, 1989; WANG, 2002) for three distances (without criteria, 25 and 50 m) were used. The coefficient of heritability estimated using the estimator of relatedness of Ritland (1996), suggest that the genetic control of the trait height is low in the regeneration, thus the natural selection as well as the artificial selection have a low potential to change the mean of the population. The estimates based on the other methods to calculate the relatedness presented low precision, indication that these methods are not adequate for the data used.

Integration of transcript and genetic maps of chromosome 16 at near-1-Mb resolution: demonstration of a 'hot-spot' for recombination at 16p12

Callen, D.; Lane, S.; Kozman, H.; Kremmidiotis, G.; Whitmore, S.; Lowenstein, M.; Doggett, N.; Kenmochi, N.; Page, D.; Maglott, D.; Nierman, W.; Murakawa, K.; Sikela, J.; Houlgatte, R.; Auffray, C.; Sutherland, G.
Fonte: Academic Press Publicador: Academic Press
Tipo: Artigo de Revista Científica
Publicado em //1995 EN
Relevância na Pesquisa
55.98%
A single mapping resource, a mouse/human somatic cell panel with average distance between breakpoints of 1.2 Mb and a potential resolution of 1 Mb, has been utilized to integrate the genetic map and a transcript map of human chromosome 16. This map includes 141 genetic markers and 200 genes and transcripts. The localization of four genes (CHEL3, TK2, TRG1, and MMP9) reported to map to chromosome 16 could not be confirmed, and for three of these localizations to other human chromosomes are reported. A correlation between genetic and physical distance over a region estimated to be 23 Mb on the short arm of chromosome 16 identified an interval demonstrating a greatly increased rate of recombination where, in females, 1 cM is equivalent to a physical distance of 100 kb.

Implementation of a parentage control system in Portuguese beef-cattle with a panel of microsatellite markers

Carolino,Inês; Sousa,Conceição O.; Ferreira,Sónia; Carolino,Nuno; Silva,Fátima S.; Gama,Luís T.
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2009 EN
Relevância na Pesquisa
56%
A study was conducted to assess the feasibility of applying a panel of 10 microsatellite markers in parentage control of beef cattle in Portugal. In the first stage, DNA samples were collected from 475 randomly selected animals of the Charolais, Limousin and Preta breeds. Across breeds and genetic markers, means for average number of alleles, effective number of alleles, expected heterozygosity and polymorphic information content, were 8.20, 4.43, 0.733 and 0.70, respectively. Enlightenment from the various markers differed among breeds, but the set of 10 markers resulted in a combined probability above 0.9995 in the ability to exclude a random putative parent. The marker-set thus developed was later used for parentage control in a group of 140 calves from several breeds, where there was the suspicion of possible faulty parentage recording. Overall, 76.4% of the calves in this group were compatible with the recorded parents, with most incompatibilities due to misidentification of the dam. Efforts must be made to improve the quality of pedigree information, with particular emphasis on information recorded at the calf's birth.

Genetic markers of insulin sensitivity and insulin secretion are associated with spontaneous postnatal growth and response to growth hormone treatment in short SGA children: the North European SGA Study (NESGAS)

Jensen, Rikke Beck; Thankamony, Ajay; Day, Felix; Scott, Robert A.; Langenberg, Claudia; Kirk, Jeremy; Donaldson, Malcolm; Ivarsson, Sten-A.; S?der, Olle; Roche, Edna; Hoey, Hilary; Juul, Anders; Ong, Ken K.; Dunger, David B.
Fonte: Endocrine Society Publicador: Endocrine Society
Tipo: Article; accepted version
EN
Relevância na Pesquisa
55.93%
This is the accepted manuscript. The final version is available at http://dx.doi.org/10.1210/jc.2014-3469.; Purpose: The wide heterogeneity in the early growth and metabolism of children born small for gestational age (SGA), both before and during growth hormone (GH) therapy, may reflect common genetic variations related to insulin secretion or sensitivity. Method: Combined multi-allele single nucleotide polymorphism (SNP) scores with known associations with insulin sensitivity or insulin secretion were analysed for their relationships with spontaneous postnatal growth and 1st year responses to GH therapy in 96 short SGA children. Results: The insulin sensitivity allele score (GS-InSens) was positively associated with spontaneous postnatal weight gain (B:0.12 SD scores per allele, 95% CI:0.01-0.23, p=0.03) and also in response to GH therapy with 1st year height velocity (0.18 cm/year per allele, 0.02-0.35, p=0.03) and change in IGF-I (0.17 SD scores per allele, 0.00-0.32, p=0.03). The association with 1st year height velocity was independent of reported predictors of response to GH therapy (adjusted p=0.04). The insulin secretion allele score (GS-InSec) was positively associated with spontaneous postnatal height gain (0.15...

A review of new PCR-based genetic markers and their utility to weed ecology

O'Hanlon, P; Peakall, Rodney; Briese, D
Fonte: Blackwell Publishing Ltd Publicador: Blackwell Publishing Ltd
Tipo: Artigo de Revista Científica
Relevância na Pesquisa
56.11%
Several recent molecular developments provide new genetic tools for addressing difficult problems in weed ecology. In this review, we describe some of the techniques and the DNA markers they generate [including amplified fragment length polymorphisms (AFLPs), simple sequence repeats (SSRs) or microsatellites, and intron-polymerase chain reaction (intron-PCR)], contrast their relative advantages and disadvantages, and discuss how they might be used in weed research. As these new markers generally reveal higher levels of variation than other techniques, they promise to improve our understanding of breeding systems, assist in determining the origin(s) of invaders, help to resolve taxonomic boundaries and relationships between closely related taxa and enable the identification of phenotypic linked markers. In addition, compared with other techniques, some of these markers may be more cost-effective, less technically demanding and more reliable. Regardless of the marker adopted, all genetic studies will benefit from careful consideration of experimental design and the formulation of testable hypotheses with practical outcomes.