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HLA-G polymorphisms in women with squamous intraepithelial lesions harboring human papillomavirus

SIMOES, Renata T.; GONCALVES, Maria Alice G.; CASTELLI, Erick C.; Mendes Junior, Celso Teixeira; BETTINI, Jussara S. R.; DISCORDE, Magali L.; DUARTE, Geraldo; QUINTANA, Silvana M.; SIMOES, Aguinaldo L.; MOREAU, Philippe; CAROSELLA, Edgardo D.; SOARES, Eds
Fonte: NATURE PUBLISHING GROUP Publicador: NATURE PUBLISHING GROUP
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
45.64%
Human papillomavirus (HPV) infection is etiologically associated with low-(LSIL) and high-grade squamous intraepithelial lesions (HSIL) and with cervical cancer. The progression or regression of the lesions may depend, among other factors, on the host heritable immune response. Because human leukocyte antigen (HLA)-G molecules are involved in the modulation of innate and adaptive immune responses, and because no previous studies have evaluated HLA-G polymorphism in patients with SIL, we conducted a study to assess the association between HLA-G polymorphisms and cervical lesions harboring HPV infection. Cervico-vaginal scrapings and blood samples were collected from 125 women with SIL (68 LSIL and 57 HSIL) and from 94 healthy women without HPV infection and cytological abnormalities. HPV type and HLA-G polymorphisms in exons 2, 3 and 8 (14 bp insertion/deletion) were evaluated by PCR methodology, and digested with restriction endonucleases. The Genepop software and the EM and PHASE algorithms were used for statistical analysis. A significant protective association was observed between the presence of the G*0103 allele and SIL and between the G0101/G0104 genotype and HSIL in the group of patients compared to control. The presence of the G0104/+14 bp and G0104/-14 bp haplotypes conferred susceptibility to SIL compared to control. In addition...

Expression of human leucocyte antigen-G primarily targets affected skin of patients with psoriasis

CARDILI, R. N.; ALVES, T. G.; FREITAS, J. C. O. C.; SOARES, C. P.; MENDES-JUNIOR, C. T.; SOARES, E. G.; DONADI, E. A.; SILVA-SOUZA, C.
Fonte: WILEY-BLACKWELL Publicador: WILEY-BLACKWELL
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
45.65%
P>Background The nonclassical human leucocyte antigen (HLA)-G molecule has been well recognized as a tolerogenic molecule and few studies have evaluated the role of the molecule in inflammatory cutaneous autoimmune diseases. Objectives To evaluate the expression of HLA-G in skin specimens of patients with psoriasis and to analyse its correlation with epidemiological and clinical variables. Methods Thirty untreated patients with psoriasis and 32 healthy individuals were enrolled. Immunohistochemistry was applied to identify HLA-G expression in formalin-fixed paraffin-embedded cutaneous skin biopsies. Results Soluble and membrane-bound HLA-G expression was detected in 30 (90%) of the skin specimens from patients presenting clinical and histopathological features of psoriasis. Although infiltrating lymphomononuclear cells of the dermis exhibited HLA-G expression, the epidermis was primarily targeted. HLA-G expression was also observed in 27% (three of 11) of the specimens that exhibited no clinical and histopathological features of psoriasis (nonaffected areas). In contrast, skin specimens obtained from healthy individuals exhibited no HLA-G expression (P < 0 center dot 0001). The intensity of HLA-G expression was not associated with type I/II psoriasis...

Low Expression of Human Histocompatibility Soluble Leukocyte Antigen-G (HLA-G5) in Invasive Cervical Cancer With and Without Metastasis, Associated With Papilloma Virus (HPV)

GUIMARAES, Marcia C. M.; SOARES, Christiane P.; DONADI, Eduardo A.; DERCHAIN, Sophie F. M.; ANDRADE, Liliana A. L. A.; SILVA, Tarsia G. A.; HASSUMI, Marcela K.; SIMOES, Renata T.; MIRANDA, Fabiana A.; LIRA, Regia C. P.; CRISPIM, Janaina; SOARES, Edson G.
Fonte: HISTOCHEMICAL SOC INC Publicador: HISTOCHEMICAL SOC INC
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
45.6%
Human leukocyte antigen-G (HLA-G) is a non-classical major histocompatibility complex class lb molecule that acts as a specific immunosuppressor. Some studies have demonstrated that human papillomavirus (HPV) seems to be involved in lower or absent HLA-G expression, particularly in cervical cancer. In this study, we performed a cross-sectional study, systematically comparing the qualitative expression of the HLA-G5 isoform in invasive cervical carcinoma (ICC), stratifying patients according to the presence [ICC with metastasis (ICC(W))) and absence [ICC without metastasis (ICC(WT))] of metastasis, correlating these findings with interference of HPV and demographic and clinical variables. Seventy-nine patients with a diagnosis of ICC were stratified into two groups: ICC(WT) (n=52 patients) and ICC(W) (n=27). Two biopsies were collected from each patient (one from the tumor lesion and one from a lymph node). Immunohistochemistry analyses were performed for the HLA-G5 isoform, for HPV detection, and virus typing. HLA-G5 isoform molecules were detected in 25 cases (31.6%), 17 (32.7%) without metastasis and 8 (29.6%) with metastasis. HPV was detected in the cervical lesions of 74 patients (93.7%), but low expression of the HLA-G5 isoform was observed in all HPV-related cases. These findings are important; however...

Expression of nonclassical molecule human leukocyte antigen-G in oral lesions

Fregonezi, Paula A. G.; Silva, Tarsia G. A.; Simoes, Renata T.; Moreau, Philipe; Carosella, Edgardo D.; Klaey, Carla P. M.; Goncalves, Maria A. G.; Soares, Edson G.; Souto, Francisco; Donadi, Eduardo A.; Soares, Christiane P.
Fonte: W B SAUNDERS CO-ELSEVIER INC; PHILADELPHIA Publicador: W B SAUNDERS CO-ELSEVIER INC; PHILADELPHIA
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
45.63%
Introduction: Human leukocyte antigen (HLA)-G is a nonclassic class I molecule that acts as a modulator of immune responses, and the expression of these molecules in virus-infected cells has been associated with subversion of the immune response. Objective: In this study, we performed a cross-sectional study, systematically comparing the expression of the HLA-G in benign, premalignant, and malignant oral lesions and correlating it with the presence of high-risk and low-risk human papillomavirus (HPV) types. Specimens and Methods: Oral biopsies were collected from 51 patients and analyzed by immunohistochemistry using anti HLA-G antibody. Human papillomavirus detection and typing from oral biopsies were obtained by polymerase chain reaction using GP5+/GP6+ and specific primers. Results: The 51 biopsies were stratified into 3 groups according to lesion grade: oral benign lesions (oral hyperplasia and papilloma, n = 16), oral premalignant lesions (oral leukoplakia with dysplasia and lichen planus, n = 17), and malignant lesions (oral squamous cell carcinoma, n = 18). Human leukocyte antigen G overexpression was mainly observed in benign and premalignant oral lesions but was not related to HPV infection (P>.05). On the other hand, HPV DNA was detected in 24 (47%) oral lesions...

Expression of human leucocyte antigen-G primarily targets affected skin of patients with psoriasis

Cardili, R. N.; Alves, T. G.; Freitas, J. C. O. C.; Soares, C. P.; Mendes-Junior, C. T.; Soares, E. G.; Donadi, E. A.; Silva-Souza, C.
Fonte: Wiley-Blackwell Publicador: Wiley-Blackwell
Tipo: Conferência ou Objeto de Conferência Formato: 769-775
ENG
Relevância na Pesquisa
45.64%
P>BackgroundThe nonclassical human leucocyte antigen (HLA)-G molecule has been well recognized as a tolerogenic molecule and few studies have evaluated the role of the molecule in inflammatory cutaneous autoimmune diseases.ObjectivesTo evaluate the expression of HLA-G in skin specimens of patients with psoriasis and to analyse its correlation with epidemiological and clinical variables.MethodsThirty untreated patients with psoriasis and 32 healthy individuals were enrolled. Immunohistochemistry was applied to identify HLA-G expression in formalin-fixed paraffin-embedded cutaneous skin biopsies.ResultsSoluble and membrane-bound HLA-G expression was detected in 30 (90%) of the skin specimens from patients presenting clinical and histopathological features of psoriasis. Although infiltrating lymphomononuclear cells of the dermis exhibited HLA-G expression, the epidermis was primarily targeted. HLA-G expression was also observed in 27% (three of 11) of the specimens that exhibited no clinical and histopathological features of psoriasis (nonaffected areas). In contrast, skin specimens obtained from healthy individuals exhibited no HLA-G expression (P < 0 center dot 0001). The intensity of HLA-G expression was not associated with type I/II psoriasis...

Helivypolide G. A novel dimeric bioactive sesquiterpene lactone

MacÍ-as, Francisco A.; López, Adriana; Varela, Rosa M.; Molinillo, José M. G.; Alves, Pedro Luis C. A.; Torres, Ascensión
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 6567-6570
ENG
Relevância na Pesquisa
45.58%
Helivypolide G was isolated from leaves of Helianthus annuus L. cv. Stella. In the course of our ongoing research for new allelochemicals from Helianthus annuus, a novel dimeric bioactive sesquiterpene lactone, helivypolide G has been isolated and characterized from the medium polar active fractions of the leaves of cultivar variety Stella. The monomers are connected through carbons C-15 of each unit and an oxygen bridge, forming an enolic oxane ring. © 2004 Elsevier Ltd. All rights reserved.

Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness

Silva, Tarsia G.; Crispim, Janaina C.O.; Miranda, Fabiana A.; Hassumi, Marcela K.; de Mello, Júlia M.Y.; Simões, Renata T.; Souto, Francisco; Soares, Edson G.; Donadi, Eduardo A.; Soares, Christiane Pienna
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 1487-1497
ENG
Relevância na Pesquisa
45.62%
Introduction: HLA-G and HLA-E are two nonclassical class I molecules, which have been well recognized as modulators of innate and adaptive immune responses, and the expression of these molecules in virus infected cells has been associated with subversion of the immune response. Objective: In this study we performed a cross-sectional study, systematically comparing the expression of HLA-G and HLA-E in benign, premalignant and malignant laryngeal lesions, correlating with demographic and clinical variables and with the presence of high-risk and low-risk HPV types. Materials and methods: Laryngeal lesions were collected from 109 patients and stratified into 27 laryngeal papillomas, 17 dysplasias, 10 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastases, 28 laryngeal carcinomas with metastasis along with their respective draining cervical lymph nodes, and 10 normal larynx specimens. The expression of HLA-G and HLA-E molecules was determined by immunohistochemistry. HPV DNA detection and typing was performed using generic and specific primers. Results: HLA nonclassical molecules showed a distinct distribution pattern, according to the larynx lesion grade. HLA-G expression increased in benign and premalignant lesions, and gradually decreased in invasive carcinomas and in respective draining cervical lymph nodes. Conversely...

A chromatographic method for the production of a human immunoglobulin G solution for intravenous use

Tanaka,K.; Sawatani,E.; Shigueoka,E.M.; Campos,T.C.X.B.; Nakao,H.C.; Dias,G.A.; Fujita,R.K.; Arashiro,F.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/1998 EN
Relevância na Pesquisa
45.58%
Immunoglobulin G (IgG) of excellent quality for intravenous use was obtained from the cryosupernatant of human plasma by a chromatographic method based on a mixture of ion-exchange, DEAE-Sepharose FF and arginine Sepharose 4B affinity chromatography and a final purification step by Sephacryl S-300 HR gel filtration. The yield of 10 experimental batches produced was 3.5 g IgG per liter of plasma. A solvent/detergent combination of 1% Tri (n-butyl) phosphate and 1% Triton X-100 was used to inactivate lipid-coated viruses. Analysis of the final product (5% liquid IgG) based on the mean for 10 batches showed 94% monomers, 5.5% dimers and 0.5% polymers and aggregates. Anticomplementary activity was 0.3 CH50/mg IgG and prekallikrein activator levels were less than 5 IU/ml. Stability at 37ºC for 30 days in the liquid state was satisfactory. IgG was stored in flasks (2.5 g/flask) at 4 to 8ºC. All the characteristics of the product were consistent with the requirements of the 1997 Pharmacopée Européenne.

Using green fluorescent protein to understand the mechanisms of G-protein-coupled receptor regulation

Ferguson,S.S.G.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/1998 EN
Relevância na Pesquisa
45.6%
G protein-coupled receptor (GPCR) activation is followed rapidly by adaptive changes that serve to diminish the responsiveness of a cell to further stimulation. This process, termed desensitization, is the consequence of receptor phosphorylation, arrestin binding, sequestration and down-regulation. GPCR phosphorylation is initiated within seconds to minutes of receptor activation and is mediated by both second messenger-dependent protein kinases and receptor-specific G protein-coupled receptor kinases (GRKs). Desensitization in response to GRK-mediated phosphorylation involves the binding of arrestin proteins that serve to sterically uncouple the receptor from its G protein. GPCR sequestration, the endocytosis of receptors to endosomes, not only contributes to the temporal desensitization of GPCRs, but plays a critical role in GPCR resensitization. GPCR down-regulation, a loss of the total cellular complement of receptors, is the consequence of both increased lysosomal degradation and decreased mRNA synthesis of GPCRs. While each of these agonist-mediated desensitization processes are initiated within a temporally dissociable time frame, recent data suggest that they are intimately related to one another. The use of green fluorescent protein from the jellyfish Aqueora victoria as an epitope tag with intrinsic fluorescence has facilitated our understanding of the relative relationship between GRK phosphorylation...

High quality human immunoglobulin G purified from Cohn fractions by liquid chromatography

Tanaka,K.; Sawatani,E.; Dias,G.A.; Shigueoka,E.M.; Campos,T.C.X.B.; Nakao,H.C.; Arashiro,F.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2000 EN
Relevância na Pesquisa
45.58%
In order to obtain intravenous immunoglobulin G (iv IgG) of high quality from F-I+II+III or F-II+III pastes prepared by the Cohn method, we developed a chromatography process using ion exchange gels, Q-Sepharose FF and CM-Sepharose FF, and Sephacryl S-300 gel filtration. Viral inactivation was performed by incubating the preparation with pepsin at pH 4.0 at 35oC for 18 h. The characteristics of 28 batches produced by us were: yield 4.3 ± 0.2 g/l plasma, i.e., a recovery of 39.1 ± 1.8%; IgG subclasses distribution: IgG1 = 58.4%, IgG2 = 34.8%, IgG3 = 4.5% and IgG4 = 2.3%; IgG size distribution was 98.4% monomers, 1.2% dimers and 0.4% polymers and protein aggregates; anticomplement activity was less than 0.5 CH50/mg IgG, and prekallikrein activator activity (PKA) was less than 5 IU/ml. These characteristics satisfied the requirements of the European Pharmacopoea edition, and the regulations of the Brazilian Health Ministry (M.S. Portaria No. 2, 30/10/1998).

Estruturação, modelagem e implantação do banco de dados geográfico para cafeicultura do sul de Minas, utilizando o modelo de dados OMT-G (Object Modelling Technique - Geographic).

VIEIRA, G. C.; ALVES, H. M. R.; SILVEIRA, S. P. S.; BERNARDES, T.; SOUZA, V. C. O.
Fonte: In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007. Publicador: In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.
Tipo: Artigo em anais de congresso (ALICE)
PT_BR
Relevância na Pesquisa
55.56%
Geotecnologias, dentre elas o sensoriamento remoto e os sistemas de informação geográfica (SIG), têm sido utilizadas para agilizar estudos e planejamento da agricultura. No caso da cultura do café, podem facilitar a avaliação da distribuição das áreas cafeeiras, com a sua quantificação e o entendimento das relações entre os sistemas de produção e o ambiente. Este trabalho descreve a estruturação, modelagem e a implantação do banco de dados geográfico do Sistema de Informação da Cafeicultura do Sul de Minas por meio do SIG-SPRING. O OMT-G foi o modelo utilizado para a modelagem dos dados geográficos gerados neste banco. Os resultados obtidos estão disponibilizados no Portal Vertical GeoSolos - http://www.epamig.br/geosolos.; 2007

A new insight about pharmaceutical dosage forms for benzathine penicillin G

Holanda e Silva, K. G; Xavier Junior, F. H; Farias, I. E. G; Caldas Neto, J. A; Silva, A. K. A. S; Nakashima Júnior, T.; Araújo, I. B.; Oliveira, A. G.; Medeiros, A. C.; Tabosa do Egito, E. S.
Fonte: Universidade Federal do Rio Grande do Norte Publicador: Universidade Federal do Rio Grande do Norte
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
45.58%
In this work, a micellar system of benzathine penicillin G (BPG) in sodium deoxycholate (NaDC) was developed and evaluated physicochemically. The solubility profile of the drug in water and buffer solutions at various pH was determined, as well as its n-octanol/water partition coefficient. The Critical Micellar Concentration of NaDC and its ability to incorporate BPG were also assessed. The study was carried out at low and high ionic strength which was adjusted by the addition of sodium chloride. The results demonstrated the ability of the micellar system to incorporate BPG, as well as to increase its apparent solubility in water. The enhancement of the solubility of BPG by the presence of NaDC micelles could be analyzed quantitatively within the framework of the pseudo-phase model. Concentration analysis showed that the micellar system could attain up to 90% incorporation of BPG. The incorporated drug is expected to exhibit improved stability, since the antibiotic enclosed in the hydrophobic core of micelles is rather shielded from the aqueous external environment

An investigation of the impacts of induced parturition, birth weight, birth order, litter size, and sow parity on piglet serum concentrations of immunoglobulin G

Nguyen, K.; Cassar, G.; Friendship, R.; Dewey, C.; Farzan, A.; Kirkwood, R.; Hodgins, D.
Fonte: American Association of Swine Veterinarians Publicador: American Association of Swine Veterinarians
Tipo: Artigo de Revista Científica
Publicado em //2013 EN
Relevância na Pesquisa
45.61%
OBJECTIVE: To determine the impacts of induced parturition, birth weight, birth order, litter size, and sow parity on piglet serum concentrations of immunoglobulin G (IgG). MATERIALS AND METHODS: In Experiment 1, sows were either induced to farrow (n = 56) or allowed to farrow naturally (n = 84). Litters of induced sows were placed immediately into a warm crèche until farrowing was complete, then all piglets were weighed and placed with the sow at the same time. Blood samples were collected at 3 days of age from one or two of the smallest pigs, one medium pig, and the largest pig in each litter for measurement of serum IgG. In Experiment 2, the firstborn and last-born piglets in 78 litters were blood sampled at 3 days of age and sera were assayed for total IgG. RESULTS: Experiment 1. Mean serum IgG concentration was higher in piglets from induced litters than in piglets from control sows (P < .001). Serum IgG concentrations increased with increased piglet weight (P < .001). Piglets from larger litters had lower serum IgG (P < .001). Serum IgG concentrations in piglets were not affected by sow parity (Experiment 1) or birth order (Experiment 2). IMPLICATIONS: Supervision of farrowing may allow for improved colostrum intake with benefits to passive immunity. However...

Dimensiones simb??licas sobre el sistema sexo/g??nero entre los ind??genas zapotecas del Istmo de Tehuantepec (M??xico)

G??mez Su??rez, ??gueda; Miano Borusso, Marinella
Fonte: Pedro G??mez Publicador: Pedro G??mez
Tipo: Artigo de Revista Científica
SPA
Relevância na Pesquisa
55.61%
La interacci??n social entre g??neros se ha ido modificando hist??ricamente, trat??ndose de un sistema relacional que var??a seg??n las coordenadas sociales, econ??micas o culturales de cada ??poca y espacio concreto. Uno de los territorios ins??litos y singulares donde la relaci??n de g??nero es excepcionalmente horizontal, se encuentra en la ciudad ind??gena zapoteca de Juchit??n, ubicada en el Istmo de Tehuantepec (M??xico), donde algunos autores han llegado a afirmar que existe un moderno sistema matriarcal. Con esta propuesta de investigaci??n dentro del ??mbito de tem??ticas en torno al g??nero y la etnicidad en Am??rica Latina, se pretende reflexionar sobre las causas que han determinado que entre la sociedad juchiteca la mujer sea protagonista de la vida social, a trav??s de la situaci??n socioecon??mica, ideol??gica y cultural dominante en esta sociedad y que la diversidad sexual se acepte e institucionalice como un "tercer g??nero".; The social interaction between the genders has been in flux historically, as this system of relations varies according to social, economic, and cultural references of each time and place. One of the lesser-known and singular territories where relations between the genders is exceptionally horizontal is in the indigenous Zapatecan city of Juchit??n...

G??nero y ciencia. Una relaci??n fruct??fera

Mass?? Guijarro, Ester
Fonte: Pedro G??mez Publicador: Pedro G??mez
Tipo: Artigo de Revista Científica
SPA
Relevância na Pesquisa
45.6%
Este breve trabajo pretende hacer una revisi??n muy sucinta acerca de la pol??mica -y f??rtil- relaci??n que actualmente se establece entre los conceptos g??nero y ciencia, as?? como de las tem??ticas fundamentales que genera o se le vinculan. As??, pretendo delimitar especialmente el concepto de g??nero y las principales corrientes en epistemolog??a feminista que lo interpretan; daremos cuenta tambi??n de algunas cr??ticas a estas tendencias y de sus tem??ticas afines m??s importantes con la Filosof??a de la Ciencia. Las principales reflexiones sobre g??nero y ciencia se desarrollan en el seno mismo de la Filosof??a de la Ciencia y en los llamados Estudios sobre Ciencia, Tecnolog??a y Sociedad (CTS). Otro objetivo importante es exponer el estado de la cuesti??n actual a este respecto.; This brief paper presents a succinct overview of the controversial -and fertile- relationship involving the concepts of gender and science as well as the fundamental themes generated or linked to it. Moreover, I attempt to define the concept of gender and the main trends in feminist epistemology to interpret this. I will also explain the criticism towards these trends in the Philosophy of Science. The main considerations on gender and science are developed in the same field of the Philosophy of Science and in the so-called Studies on Science...

G??nero, profesiones sanitarias y salud p??blica

Ortiz G??mez, Teresa; Birriel-Salcedo, Johanna; Ortega del Olmo, Rosa
Fonte: Universidade de Granada Publicador: Universidade de Granada
Tipo: Artigo de Revista Científica
SPA
Relevância na Pesquisa
45.6%
En este trabajo llevamos a cabo una revisi??n de los principales resultados de la investigaci??n hist??rico-social sobre g??nero y pr??cticas de salud, proponemos un modelo de aplicaci??n de la categor??a de g??nero al estudio de las profesiones sanitarias y analizamos algunas formas actuales de desigualdades de g??nero en asociaciones y publicaciones espa??olas de salud p??blica. Las principales conclusiones resaltan distintos aspectos: la construcci??n hist??rica de identidades profesionales sexuadas; la existencia de una segregaci??n vertical en las sociedades cient??ficas y en los equipos editoriales de las revistas; la existencia de pr??cticas androc??ntricas en las revistas cient??ficas que se manifiestan en el estilo de firma mediante la inicial del nombre; la inexistencia en las sociedades cient??ficas de datos recogidos por sexo; las dificultades que todo ello supone para las investigaciones cuantitativas aplicando la variable sexo y una perspectiva de g??nero, y la necesidad de fomentar la investigaci??n cualitativa sobre el tema.; In this article we review the main results of historical-social research on gender and medical practice, propose a model for applying a gender perspective to the study of healthcare professions...

Identidad socioprofesional e identidad de g??nero. Un caso emp??rico

T??llez Infantes, Anastasia
Fonte: Pedro G??mez Publicador: Pedro G??mez
Tipo: Artigo de Revista Científica
SPA
Relevância na Pesquisa
45.58%
El presente trabajo se centra en el an??lisis de la identidad socioprofesional y la identidad de g??nero de las trabajadoras de una industria tradicional. El objetivo principal es demostrar que la participaci??n de los sujetos en determinados procesos productivos genera en ellos una espec??fica identidad que se manifiesta en su realidad social cotidiana a trav??s de unos rasgos culturales compartidos tales como su argot profesional, la percepci??n del tiempo, los saberes espec??ficos, las destrezas, la forma de interpretar parte de su mundo, las relaciones laborales, etc. Junto a ello se constata, a trav??s de un caso de estudio emp??rico, c??mo las representaciones ideol??gicas en relaci??n al g??nero que existen en nuestra sociedad modelan la identidad de g??nero de las mujeres analizadas a la vez que se convierten en uno de los factores fundamentales en la estructuraci??n de este proceso productivo.; The present study focuses on the analysis of the professional identity and the gender identity of the workers of a traditional industry. The main objective is to demonstrate that the participation of the subjects in certain productive processes generates in them a specific identity that is manifested in its daily social reality, through some shared cultural features such as professional jargon...

Frequency of the ATM IVS10-6T -> G variant in Australian multiple-case breast cancer families

Lindeman, G.; Hiew, M.; Visvader, J.; Leary, J.; Field, M.; Gaff, C.; McKinlay Gardner, R.; Trainor, K.; Cheetham, G.; Suthers, G.; Kirk, J.
Fonte: Biomed Central Ltd Publicador: Biomed Central Ltd
Tipo: Artigo de Revista Científica
Publicado em //2004 EN
Relevância na Pesquisa
45.62%
BACKGROUND: Germline mutations in the genes BRCA1 and BRCA2 account for only a proportion of hereditary breast cancer, suggesting that additional genes contribute to hereditary breast cancer. Recently a heterozygous variant in the ataxia–telangiectasia mutated (ATM) gene, IVS10-6T→G, was reported by an Australian multiple-case breast cancer family cohort study (the Kathleen Cuningham Foundation Consortium for Research into Familial Breast Cancer) to confer a substantial breast cancer risk. Although this variant can result in a truncated ATM product, its clinical significance as a high-penetrance breast cancer allele or its role as a low-penetrance risk-modifier is controversial. METHODS: We determined the frequency of ATM IVS10-6T→G variants in a cohort of individuals affected by breast and/or ovarian cancer who underwent BRCA1 and BRCA2 genetic testing at four major Australian familial cancer clinics. RESULTS: Seven of 495 patients (1.4%) were heterozygous for the IVS10-6T→G variant; the carrier rate in unselected Australian women with no family history of breast cancer is reported to be 6 of 725 (0.83%) (P = 0.4). Two of the seven probands also harboured a pathogenic BRCA1 mutation and one patient had a BRCA1 unclassified variant of uncertain significance. CONCLUSION: These findings indicate that the ATM IVS10-6T→G variant does not seem to occur at a significantly higher frequency in affected individuals from high-risk families than in the general population. A role for this variant as a low-penetrance allele or as a modifying gene in association with other genes (such as BRCA1) remains possible. Routine testing for ATM IVS10-6T→G is not warranted in mutation screening of affected individuals from high-risk families.; Geoffrey J Lindeman...

Haplotype frequencies based on eight polymorphic sites at the 3' untranslated region of the HLA-G gene in individuals from two different geographical regions of Brazil

Lucena-Silva, N.; Monteiro, A. R.; de Albuquerque, R. S.; Gomes, R. G.; Junior, Celso Teixeira Mendes; Castelli, E. C.; Donadi, Eduardo Antonio
Fonte: WILEY-BLACKWELL; MALDEN Publicador: WILEY-BLACKWELL; MALDEN
Tipo: Artigo de Revista Científica
ENG
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The Brazilian population represents an admixture of native Amerindians, Portuguese settlers and Africans who were brought as slaves during the colonization period that began in the 16th century and was followed by waves of immigrations of Europeans and Asians in the 20th century. The contribution of these different ethnic groups to the constitution of Brazilian populations from different geographic regions is variable and, in addition to environmental factors, might act by determining different allele profiles among Brazilian populations from different regions. We studied polymorphic sites at the 3' untranslated region of the HLA-G gene in individuals from a Northeastern Brazilian region and compared them to our previously published data about a Southeastern Brazilian region, located at a distance of 2589 km. Our results showed that most polymorphic sites present a similar distribution in both populations, except for the lower frequency of the +3003C allele in the Northeastern population compared to the Southeastern population. Although differences in genotypic distribution were only significant for the +3003 locus (P = 0.0201), the diversity of haplotypes was distinct for each population. These results are important for casecontrol studies on the association of human leucocyte antigen-G polymorphism with disease and also in terms of the genetic structure of two distinct Brazilian populations.; Program for Technological Development in Tools for Health-PDTISFIOCRUZ; Program for Technological Development in Tools for HealthPDTISFIOCRUZ; Instituto de Hematologia do Nordeste - IHENE; Instituto de Hematologia do Nordeste IHENE; CNPq [150572/2011-5]; CNPq; FACEPE; FACEPE [APQ-0184-4.01/10]

IFN-g in human Chagas' disease: protection or pathology?

Bahia-Oliveira,L.M.G.; Gomes,J.A.S.; Rocha,M.O.C.; Moreira,M.C.V.; Lemos,E.M.; Luz,Z.M.P.; Pereira,M.E.S.; Coffman,R.L.; Dias,J.C.P.; Cançado,J.R.; Gazzinelli,G.; Corrêa-Oliveira,R.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/1998 EN
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An apparently paradoxical role for IFN-g in human Chagas' disease was observed when studying the pattern of cytokine production by peripheral blood mononuclear cells (PBMC) obtained from two groups of chagasic patients after specific stimulation with Trypanosoma cruzi-derived antigens. The groups studied were 1) patients treated with benznidazole during the acute phase of Trypanosoma cruzi infection and 2) chronically infected untreated patients. In the treated group, higher levels of IFN-g were produced by PBMC from individuals cured after treatment when compared to non-cured patients. In contrast, in the chronically infected group (not treated) higher levels of IFN-g were produced by PBMC from cardiac patients in comparison with asymptomatic (indeterminate) patients. This apparently paradoxical role for IFN-g in human Chagas' disease is discussed in terms of the possibility of a temporal difference in IFN-g production during the initial stages of the infection (acute phase) in the presence or absence of chemotherapy. The maintenance of an immune response with high levels of IFN-g production during the chronic phase of the infection may favor cure or influence the development of the cardiac form of the disease