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Expression of cell cycle regulatory proteins in epithelial components of dental follicles

Matsumoto, Mariza A.; Nary Filho, Hugo; Jorge, Francine M.; Salvadori, Daisy Maria Favero; Marques, Mariangela E. A.; Ribeiro, Daniel A.
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica Formato: 127-131
ENG
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Dental follicle is a component of tooth germs, which remain adjacent to the crown of unerupted or impacted teeth. Under the influence of pathologic changes, however, dental follicles that possess reduced epithelium can proliferate into stratified squamous epithelium as far as originate dental cysts. In order to clarify the role of apoptosis and cellular proliferation herein, expression of p53 and PCNA was examined in epithelial components of dental follicles associated with impacted third molars by means of immunohistochemistry. A total of 40 cases was included in this study being 22 cases with reduced epithelium and 18 cases with stratified epithelium. Expression of p53 expression was weak or not detected in dental follicles with reduced and stratified squamous epithelium. By contrast, PCNA positive cells were evidenced in basal and supra basal layers of the stratified squamous epithelium and in reduced epithelium of dental follicles, but without any significant statistically differences between them (P > 0.05). In conclusion, these data suggest that dental follicles possess proliferative activity as depicted by PCNA-positive nuclei in some epithelial cells. However, the biological behavior of dental follicles during the late stage of dental eruptive process may not be associated with deregulation of death and/or cell proliferation.

Morphologic and quantitative evaluation of preantral follicles of buffalo (Bubalus bubalis) in different reproductive phases

Carvalho, F. C. A.; Oba, E.; Leal, L. S.
Fonte: Pagepress Publ Publicador: Pagepress Publ
Tipo: Artigo de Revista Científica Formato: 691-693
ENG
Relevância na Pesquisa
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The objectives of the present study were to estimate the population of the normal and degenerated preantral follicles of buffaloes in different reproductive phases and to classify the different types of degeneration of ovarian preantral follicles. For this, 18 ovaries were divided in three groups: prepubertal, non pregnat and pregnant adult females. The ovaries were collected from a slaughterhouse (Frigol - Brazil) and processed for classic histological examination. The follicular population was estimated according to Cahill et al. (1979) and calculated according to Gougeon & Chainy (1987). Young buffaloes, pregnant and non pregnat adults presented an average number of 15.5, 3.7 and 8.9 preantral follicles with normal morphology and type I (the degeneration was observed just in oocyte) and II (oocyte and granulosa cells were degenerated) degeneration, respectively. This experiment demonstrated that the proportions of primordial, primary and secondary follicles were affected by reproductive phase. A significant difference was observed considering the proportions of preantral follicles with normal morphology and Type I degeneration in all reproductive phases evaluated. The primary follicles presented more percentage of Type I degeneration that the others follicles and the primordial and primary follicles presented more percentage of Type II degeneration in three reproductive phases evaluated.

Viability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue

Tsuribe, Patricia Miyuki; Monte Gobbo, Carlos Alberto; Landim e Alvarenga, Fernanda da Cruz
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 1976-1983
ENG
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37.03%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 02/02701-1; Objective: To develop an efficient technique to preserve primordial follicles from cryopreserved ovarian tissue.Design: Frozen-thawed and fresh preantral follicles were mechanically isolated for viability testing, and their morphology was histologically analyzed.Setting: Laboratory of Animal Reproduction, Faculty of Veterinary Medicine and Zootechny, University State of São Paulo, Brazil.Animal(s): Lambs 12-24 months of age.Intervention(s): Ovarian cortical fragments were prepared for cryoprotectant toxicity testing, freezing and thawing procedures, and in vitro culture.Main Outcome Measure(s): Histologic structure and follicular viability.Result(s): on day zero, no morphologic differences were observed between follicles isolated from fresh tissue and those treated with the cryopreservatives ethylene glycol (EG) and dimethyl sulfoxide (DMSO) and subjected to freezing. Even so, frozen follicles treated with DMSO + EG showed dark staining, indicating degeneration. on day zero, the follicular viability was similar between the control group (78.9%) and those treated with EG (77%) and frozen with EG (75%). After 10 days in culture, a reduced percentage of follicles was considered viable in all groups. This decrease was accentuated in those treated with DMSO (37.5% and 35.2% in those exposed to and frozen with DMSO...

Fibroblast growth factor-10 maintains the survival and promotes the growth of cultured goat preantral follicles

Chaves, R. N.; Lima-Verde, I. B.; Celestino, J. J. H.; Duarte, A. B. G.; Alves, A. M. C. V.; Matos, M. H. T.; Campello, C. C.; Name, K. P. O.; Bao, S. N.; Buratini, J.; Figueiredo, J. R.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 249-258
ENG
Relevância na Pesquisa
37.12%
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); The aim of the present study was to investigate the effects of fibroblast growth factor-10 (FGF-10) on the survival, activation (transition from primordial to primary follicles), and growth of goat preantral follicles cultured in vitro. Pieces of ovarian cortex were cultured for 1 and 7 d in the absence or presence of FGF-10 (0, 1, 10, 50, 100, and 200 ng/mL). Noncultured and cultured tissues were processed and analyzed by histology, transmission electron microscopy, and viability testing. Results showed that after 7 d, a greater percentage (79.9%) of morphologically normal follicles (containing an oocyte with regular shape and uniform cytoplasm, and organized layers of granulosa cells without a pyknotic nucleus) was observed when cultured with 50 ng/mL of FGF-10 when compared with other concentrations of FGF-10 (0 ng/mL, 67.3%; 1 ng/mL, 68.2%; 10 ng/mL, 63.3%; 100 ng/mL, 64.4%; 200 ng/mL, 52.7%). Ultrastructural analyses and viability testing using fluorescent markers confirmed the follicular integrity of FGF-10 (50 ng/mL)-treated fragments after 7 d of culture. After 7 d, all FGF-10 concentrations reduced the percentage of primordial follicles and increased the percentage of developing follicles. In the presence of 50 ng/mL of FGF-10...

Interactions of indole acetic acid with EGF and FSH in the culture of ovine preantral follicles

Andrade, E. R.; Seneda, M. M.; Alfieri, A. A.; de Oliveira, J. A.; Bracarense, APFRL; Figueiredo, JR; Toniolli, R.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 1104-1113
ENG
Relevância na Pesquisa
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The mechanisms that regulate the gradual exit of ovarian follicles from the non-growing, primordial pool are very poorly understood. The objective of this study was to evaluate the effects of adding indole acetic acid (IAA), epidermal growth factor (EGF) and follicle stimulating hormone (FSH) to the media for in vitro culture of ovine ovarian fragments and determine their effects on growth activation and viability of preantral follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed in Bouin (control). The other fragments were cultured for 2 or 6 days in culture plates with: minimum essential medium (MEM) supplemented with insulin-transferrin-selenium (ITS), pyruvate, glutamine, hypoxantine, bovine serum albumine and antibiotics (MEM+); MEM+ plus IAA (40 ng/mL); MEM+ plus EGF (100 ng/mL); MEM+ plus FSH (100 ng/mL); MEM+ plus IAA + EGF; MEM+ plus IAA + FSH; MEM+ plus EGF + FSH; or MEM+ plus IAA + EGF + FSH. After 2 or 6 days of culture in each treatment, the pieces of ovarian cortex were fixed in Bonin for histological examination. Follicles were classified as primordial or developing (primary and secondary) follicles. Compared to the control, in all media tested, the percentages of primordial follicles were reduced (P < 0.05) and the percentages of developing follicles were increased (P < 0.05) after 2 or 6 days of culture. Furthermore...

Effects of ascorbic acid on in vitro culture of bovine preantral follicles

Andrade, Evelyn R.; Van Den Hurk, Robert; Lisboa, Lívia A.; Hertel, Mariana F.; Melo-Sterza, Fabiana A.; Moreno, Kleber; Bracarense, Ana Paula F.R.L.; Landim-Alvarenga, Fernanda C.; Seneda, Marcelo M.; Alfieri, Amauri A.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 379-388
ENG
Relevância na Pesquisa
37.12%
The objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine their effects on growth activation and viability of early-stage follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed (control) and the other fragments were cultured in minimum essential medium (MEM) supplemented or not with various doses of ascorbic acid. Ovarian tissue was processed for histology, transmission electron microscopy (TEM) and immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA). Compared with control fragments, the percentage of primordial follicles was reduced (p < 0.05) and the percentage of growing follicles had increased (p < 0.05) in cultured cortical fragments, independent of the tested medium or incubation time. Furthermore, compared with control tissue, culture of ovarian cortex for 8 days reduced the percentages of healthy, viable follicles (p < 0.05), but not when cultures were supplemented with 25, 50 or 100 μg/ml of ascorbic acid. Ultrastructural and immunohistochemical analysis of 8 day cultured ovarian cortical fragments, however, showed the integrity and viability of follicles only when fragments were cultured in presence of 50 μg/ml of ascorbic acid. In conclusion...

Human follicle stimulating hormone (hFSH) and thyroxine (T4) in survival maintenance and in vitro growth promotion of caprine preantral follicles

Costa, Sanely Lourenço da; Costa, Eduardo Paulino da; Pereira, Emílio César Martins; Gonçalves, Wagner Gonzaga; Silva, Talita Fernandes da; Queiroz, Vanessa Lopes Dias
Fonte: Universidade Federal de Goiás Publicador: Universidade Federal de Goiás
Tipo: Artigo de Revista Científica Formato: 298-311
ENG
Relevância na Pesquisa
37.03%
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); The aim of this study was to investigate the interaction of human FSH (10ng/ml) with T4 (20ng/mL) on survival, activation and growth of preantral follicles cultured in vitro for 28 days. Fragments of non-cultured and cultured ovarian tissue were processed for classic histology and transmission electron microscopy. The results showed a reduction in the survival rate in all the media tested (one to 28 days) when compared to the fresh control. However the treatment with T4/hFSH for seven days of culture maintained the rate similar to the control. The media tested by one and 28 days reduced the percentage of primordial follicles in all periods of culture. However, T4/hFSH on day one of culture remained similar to the fresh control. None of the media were able to keep the percentage of the developing follicles. It was observed that the follicular diameter in the medium with T4/hFSH remained similar to the fresh control. The ultrastructural analysis confirmed the integrity of follicles cultured for seven days in a medium supplemented with T4/hFSH. In conclusion, the medium with T4/hFSH is able to maintain the survival, promote the activation, and the ultrastructural integrity of caprine preantral follicles for until seven days.; O objetivo deste estudo foi investigar a interação do FSH humano (10 ng/mL) com T4 (20 ng/mL) na sobrevivência...

Histological study of capuchin monkey (Cebus apella) ovarian follicles

Domingues,Sheyla Farhayldes Souza; Diniz,Luiz Viana; Furtado,Sonia Helena Costa; Ohashi,Otavio Mitio; Rondina,David; Silva,Lúcia Daniel Machado
Fonte: Instituto Nacional de Pesquisas da Amazônia Publicador: Instituto Nacional de Pesquisas da Amazônia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2004 EN
Relevância na Pesquisa
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The present study aimed to obtain quanti-qualitative data about the follicular ovarian population in Cebus apella females. Seven ovaries were obtained from 4 C. apella adult females. The ovaries were subjected to light microscopy. The number of preantral and antral follicles for each ovary was estimated using the Fractionator method. The preantral follicles were classified into primordial, transitional, primary and secondary follicles. Antral follicles were those that presented an antral cavity. All counted follicles were classified as normal or degenerated. The diameter of the follicles, oocytes and their nuclei were determined to accompany the follicular development. All results were represented as mean ± SE. The number of preantral follicles was 56,938 ± 21,888 and 49,133 ± 26,896 for the right and left ovaries, respectively. The percentage of normal follicles was 80 ± 4.95%. The follicular diameter ranged from 22 ± 0.5 µm to 61.2 ± 4.0 µm. Regarding the antral follicles, the number of normal and degenerate follicles per ovary were 60.0 ± 19.0 and 3 ± 1.8 follicles, respectively. The antral follicular diameter was 514.4 + 56.6 µm. In conclusion, the information obtained in this study can be used as a parameter for subsequent in vivo or in vitro studies about folliculogenesis in non-human neotropical primates of the C. apella species.

Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro

Lima-Verde,Isabel B.; Matos,Maria H.T.; Celestino,Juliana J.H.; Rossetto,Rafael; Name,Khesller P.O.; Báo,Sônia N.; Campello,Cláudio C.; Figueiredo,José R.
Fonte: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Publicador: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2012 EN
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We investigated the effects of progesterone and follicle stimulating hormone (FSH) on survival and growth of caprine preantral follicles. Pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (MEM) alone or containing progesterone (1, 2.5, 5, 10 or 20ng/mL), FSH (50ng/mL) or the interaction between progesterone and FSH. Fresh (non-cultured control) and cultured ovarian tissues were processed for histological and ultrastructural studies. After 7 days the addition of FSH to all progesterone concentrations maintained the percentage of normal follicles similar to fresh control. At day 7 of culture, a higher percentage of developing follicles was observed only in 2.5ng/ml of progesterone associated with FSH or 10ng/ml of progesterone alone when compared with control. From day 1 to day 7 of culture, a significant increase in the percentage of developing follicles was observed in MEM and 2.5ng/ml of progesterone + FSH. In addition, after 7 days, in all treatments, there was a significant increase in follicular diameter when compared with control, except for MEM alone and in 5ng/ml of progesterone + FSH or 10ng/ml of progesterone alone. Ultrastructural studies confirmed follicular integrity after 7 days of culture in 2.5ng/ml of progesterone with FSH. In conclusion...

Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

Costa,Sanely L.; Costa,Eduardo P.; Pereira,Emílio C.M.; Benjamin,Laércio A.; Rodrigues,Marcelo T.; Mendes,Vívian R.A.; Silva,Talita F.
Fonte: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Publicador: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2014 EN
Relevância na Pesquisa
37.07%
The aim of this study was to investigate the effects of the insulin-like growth factor -I (IGF-I) on survival, activation (transition from primordial to primary follicles) and growth of caprine preantral follicles cultured in vitro. Fragments of ovarian cortex were cultured for one and seven days in the absence or presence of IGF-I (0, 50 and 100ng/ml). The non-cultured and cultured tissues were processed and analyzed by histology and transmission electron microscopy. The culture for one day in a medium with 100ng/ml of IGF-I showed 86.7% of morphologically normal follicles. These results were similar (P>0.05) to the percentage of normal follicles found in the control (96.7%). It was also found that this medium increased the percentage of follicular activation (developing follicles) with one day of culture. The oocyte and follicular diameters remained similar to the control by culturing for one day in a medium containing 100ng/ml of IGF-I. The ultrastructural analysis did not confirm the integrity of the follicular fragments in a medium containing IGF-I (100ng/ml) after one and seven days of culture. In conclusion, this study demonstrated that the addition of 100 ng/ml of IGF-I in the culture medium enables the development of preantral follicles of goats with one day of culture. However...

Degeneration rate of goat primordial follicles maintained in TCM 199 or PBS at different temperatures and incubation times

Silva,José Roberto Viana; Brasil,Alline Ferreira; Santos,Regiane Rodrigues dos; Costa,Sônia Helena Furtado; Rodrigues,Ana Paula Ribeiro; Ferreira,Marcos Antônio Leal; Machado,Vanessa Porto; Figueiredo,José Ricardo de
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2003 EN
Relevância na Pesquisa
36.98%
The present work has investigated the degeneration rate of goat primordial follicles in situ after preservation in PBS or TCM 199 at different temperatures and incubation times. For each animal the ovarian pair was divided into 19 fragments. One ovarian fragment was taken randomly and immediately fixed (control). The other 18 ovarian fragments were randomly distributed in tubes containing PBS or TCM 199 and stored at 4º, 20º or 39ºC for 4, 12 or 24h. The storage of ovarian fragments in PBS or TCM 199 at 20ºC for 12h and 24h or at 39ºC, in all incubation times tested, increased significantly the percentage of degenerated primordial follicles (P<0.05). In contrast, for both media tested the degeneration rate of primordial follicles preserved at 4ºC for up to 24h and at 20ºC for 4h was similar to control values (P>0.05). In conclusion, this study shows that PBS was as efficient as TCM 199 in the preservation of goat primordial follicles in situ, being the best results observed at 4ºC.

Goat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro

Leitão,Cintia Camurça Fernandes; Costa,José Jackson Nascimento; Saraiva,Márcia Viviane Alves; Araújo,Valdevane Rocha; Figueiredo,José Ricardo; Hurk,Robert van den; Silva,José Roberto Viana
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2013 EN
Relevância na Pesquisa
37.16%
This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of mRNA for inhibin-ßA, and activin-A increased the levels of FSH-R mRNA. In conclusion, mRNA for inhibin-ßA is expressed at different levels in pre-antral and antral follicles and activin-A acts as a stimulator of the FSH-R expression in goat follicles. On its turn, the expression of inhibin-ßA is stimulated by FSH, which together with activin-A promotes secondary follicle growth in-vitro.

Preservation of goat preantral follicles in saline or coconut water solution

Costa,Sonia Helena Furtado; Santos,Regiane Rodrigues dos; Ferreira,Marcos Antônio Leal; Machado,Vanessa Porto; Rodrigues,Ana Paula Ribeiro; Ohashi,Otávio Mitio; Figueiredo,José Ricardo de
Fonte: Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo Publicador: Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2002 EN
Relevância na Pesquisa
37.03%
The present study investigated the efficiency of saline solution and coconut water solution in the preservation of goat preantral follicles enclosed in ovarian tissue, at different temperatures and for different incubation periods. At the slaughterhouse, the ovarian pair was divided into 19 fragments; one ovarian fragment was immediately fixed for histology (control-time zero). The other 18 ovarian fragments were preserved in both solutions at 4ºC, 20ºC or 39ºC for 4 h, 12 h or 24 h. The histological analysis showed that the storage of ovarian fragments in both solutions at 4ºC for up to 24 h kept the percentage of normal preantral follicles similar to the control values. In contrast, preservation at 20°C or 39ºC, in either solution, reduced significantly the percentage of normal preantral follicles compared to the control values, except in saline solution at 20ºC for 4 h or in coconut water solution at 20ºC for 4 h and 12 h. In conclusion, this study shows that both solutions can be used with the same efficiency to preserve goat preantral follicles at 4°C, irrespective of the incubation time. However, to preserve goat preantral follicles at higher temperatures, coconut water solution is recommended.

HUMAN FOLLICLE STIMULATING HORMONE (hFSH) AND THYROXINE (T4) IN SURVIVAL MAINTENANCE AND IN VITRO GROWTH PROMOTION OF CAPRINE PREANTRAL FOLLICLES

Costa,Sanely Lourenço da; Costa,Eduardo Paulino da; Pereira,Emílio César Martins; Gonçalves,Wagner Gonzaga; Silva,Talita Fernandes da; Queiroz,Vanessa Lopes Dias
Fonte: Universidade Federal de Goiás Publicador: Universidade Federal de Goiás
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2015 EN
Relevância na Pesquisa
37.03%
The aim of this study was to investigate the interaction of human FSH (10ng/ml) with T4 (20ng/mL) on survival, activation and growth of preantral follicles cultured in vitro for 28 days. Fragments of non-cultured and cultured ovarian tissue were processed for classic histology and transmission electron microscopy. The results showed a reduction in the survival rate in all the media tested (one to 28 days) when compared to the fresh control. However the treatment with T4/hFSH for seven days of culture maintained the rate similar to the control. The media tested by one and 28 days reduced the percentage of primordial follicles in all periods of culture. However, T4/hFSH on day one of culture remained similar to the fresh control. None of the media were able to keep the percentage of the developing follicles. It was observed that the follicular diameter in the medium with T4/hFSH remained similar to the fresh control. The ultrastructural analysis confirmed the integrity of follicles cultured for seven days in a medium supplemented with T4/hFSH. In conclusion, the medium with T4/hFSH is able to maintain the survival, promote the activation, and the ultrastructural integrity of caprine preantral follicles for until seven days.

Transcriptome profiling of granulosa cells from bovine ovarian follicles during atresia

Hatzirodos, N.; Hummitzsch, K.; Irving-Rodgers, H.; Harland, M.; Morris, S.; Rodgers, R.
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em //2014 EN
Relevância na Pesquisa
37.12%
Background: The major function of the ovary is to produce oocytes for fertilisation. Oocytes mature in follicles surrounded by nurturing granulosa cells and all are enclosed by a basal lamina. During growth, granulosa cells replicate and a large fluid-filled cavity (the antrum) develops in the centre. Only follicles that have enlarged to over 10 mm can ovulate in cows. In mammals, the number of primordial follicles far exceeds the numbers that ever ovulate and atresia or regression of follicles is a mechanism to regulate the number of oocytes ovulated and to contribute to the timing of ovulation. To better understand the molecular basis of follicular atresia, we undertook transcriptome profiling of granulosa cells from healthy (n = 10) and atretic (n = 5) bovine follicles at early antral stages (< 5 mm). Results: Principal Component Analysis (PCA) and hierarchical classification of the signal intensity plots for the arrays showed primary clustering into two groups, healthy and atretic. These analyses and size-frequency plots of coefficients of variation of signal intensities revealed that the healthy follicles were more heterogeneous. Examining the differentially-expressed genes the most significantly affected functions in atretic follicles were cell death...

Transcriptome profiling of granulosa cells of bovine ovarian follicles during growth from small to large antral sizes

Hatzirodos, N.; Irving-Rodgers, H.; Hummitzsch, K.; Harland, M.; Morris, S.; Rodgers, R.
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Publicado em //2014 EN
Relevância na Pesquisa
37.15%
Background: At later stages of folliculogenesis, the mammalian ovarian follicle contains layers of epithelial granulosa cells surrounding an antral cavity. During follicle development granulosa cells replicate, secrete hormones and support the growth of the oocyte. In cattle, the follicle needs to grow > 10 mm in diameter to allow an oocyte to ovulate, following which the granulosa cells cease dividing and differentiate into the specialised cells of the corpus luteum. To better understand the molecular basis of follicular growth and granulosa cell maturation, we undertook transcriptome profiling of granulosa cells from small (< 5 mm; n = 10) and large (> 10 mm, n = 4) healthy bovine follicles using Affymetrix microarrays (24,128 probe sets).Results: Principal component analysis for the first two components and hierarchical clustering showed clustering into two groups, small and large, with the former being more heterogeneous. Size-frequency distributions of the coefficient of variation of the signal intensities of each probe set also revealed that small follicles were more heterogeneous than the large. IPA and GO enrichment analyses revealed that processes of axonal guidance, immune signalling and cell rearrangement were most affected in large follicles. The most important networks were associated with: (A) Notch...

Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

Costa, Sanely L.; Costa, Eduardo P.; Pereira, Emilio C. M.; Benjamin, Laercio A.; Rodrigues, Marcelo T.; Mendes, Vivian R. A.; Silva, Talita F.
Fonte: Revista Pesquisa Veterinaria Brasileira Publicador: Revista Pesquisa Veterinaria Brasileira
Tipo: Artigo de Revista Científica Formato: 1037-1044
ENG
Relevância na Pesquisa
37.07%
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); The aim of this study was to investigate the effects of the insulin-like growth factor -I (IGF-I) on survival, activation (transition from primordial to primary follicles) and growth of caprine preantral follicles cultured in vitro. Fragments of ovarian cortex were cultured for one and seven days in the absence or presence of IGF-I (0, 50 and 100ng/ml). The non-cultured and cultured tissues were processed and analyzed by histology and transmission electron microscopy. The culture for one day in a medium with 100ng/ml of IGF-I showed 86.7% of morphologically normal follicles. These results were similar (P>0.05) to the percentage of normal follicles found in the control (96.7%). It was also found that this medium increased the percentage of follicular activation (developing follicles) with one day of culture. The oocyte and follicular diameters remained similar to the control by culturing for one day in a medium containing 100ng/ml of IGF-I. The ultrastructural analysis did not confirm the integrity of the follicular fragments in a medium containing IGF-I (100ng/ml) after one and seven days of culture. In conclusion...

The physiology of skin and wool follicles of finewool and strongwool merinos.

Hocking Edwards, Janelle Elizabeth
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //1994
Relevância na Pesquisa
37.1%
Wool production differences between sheep maintained under similar environmental conditions appear to reside in the functioning of individual follicles. The investigations presented in this thesis utilise the differing wool producing abilities of two strains of Merino, finewool and strongwool Merinos. The relationships between wool production (on both a unit area and individual follicle basis) and skin and follicle characteristics, blood flow and microvasculature of the skin and incorporation of ³H-glucose and ³⁵S-cystine by the skin were examined. The differences in the structure and function of wool follicles and their association with fibre production were examined in 6 finewool Merinos (Camden Park) and 6 strongwool Merinos (East Bungaree). The strongwool Merinos produced 2.4 times more wool per unit area of skin and 3.5 times the volume of fibre per follicle than the finewool Merinos, when both groups were maintained under similar environmental conditions. The finewool Merinos had a higher follicle density, but a lower average volume of germinative tissue in the follicle bulb and the skin than the strongwool Merinos. The rate of cell production in the follicle bulb was greater in the strongwool Merinos than the finewool Merinos...

Isolamento e caracterização ultraestrutural de folículos pré-antrais de vacas da raça Nelore (Bos taurus indicus); Isolation and ultrastructural characterization of preantral follicles in the Nelore breed cows (Bos taurus indicus)

Basso, Andréa Cristina; Esper, Cesar Roberto
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; ; Formato: application/pdf
Publicado em 01/01/2002 POR
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37.18%
Folículos pré-antrais de 41 vacas da raça Nelore foram quantitativa e ultraestruturalmente descritos neste estudo. Uma média de 35.539,20 folículos pré-antrais foram isolados mecanicamente ("Tissue Chopper") por animal. Os folículos foram processados para microscopia eletrônica de transmissão. Os folículos primordiais apresentaram um oócito rodeado por uma camada de células granulosas (CGs) achatadas, com algumas tendendo à forma cuboidal. As demais fases de desenvolvimento foram classificadas como folículos primários, com uma camada de CGs cuboidais, e secundários, com mais de duas camadas de CGs cuboidais. Os folículos primordiais apresentaram oócito evidente, com núcleo excêntrico e nucléolo bem definido, cercado por regiões de cromatina condensada. As organelas ao redor do núcleo eram, predominantemente, mitocôndrias arredondadas. Folículos em desenvolvimento apresentavam organelas mais dispersas e numerosas, com mitocôndrias alongadas. As comunicações entre o oócito e as CGs mantinham-se por zonas de aderência, "coated pits" e vesículas de endocitose. A zona pelúcida (ZP) começava a aparecer em folículos primários, mostrando microvilos pequenos e vesículas penetrando na ZP. Os folículos secundários apresentavam aglomerados de grânulos corticais em associação a complexos de Golgi. Concluímos que o método mecânico de isolamento fornece quantidades suficientes de folículos pré-antrais de ovários de vacas da raça Nelore e...

Conservação de folículos pré-antrais caprinos em solução salina ou à base de água de coco; Preservation of goat preantral follicles in saline or coconut water solution

Costa, Sonia Helena Furtado; Santos, Regiane Rodrigues dos; Ferreira, Marcos Antônio Leal; Machado, Vanessa Porto; Rodrigues, Ana Paula Ribeiro; Ohashi, Otávio Mitio; Figueiredo, José Ricardo de
Fonte: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia Publicador: Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; ; Formato: application/pdf
Publicado em 01/01/2002 ENG
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O presente estudo investigou a eficiência da solução salina e solução à base de água de coco na preservação de folículos pré-antrais inclusos em tecido ovariano, em diferentes temperaturas e diferentes tempos de incubação. No abatedouro, o par ovariano foi dividido em 19 fragmentos; um fragmento ovariano foi imediatamente fixado para histologia clássica (controle-tempo zero). Os outros 18 fragmentos ovarianos foram conservados em ambas as soluções a 4ºC, 20ºC ou 39ºC por 4 h, 12 h ou 24 h. A análise histológica mostrou que a conservação de fragmentos ovarianos em ambas as soluções a 4ºC por até 24 h mantém a percentagem de folículos pré-antrais normais similar aos valores do controle. Ao contrário, a conservação a 20°C ou 39ºC, em ambas as soluções, reduziu significativamente a percentagem de folículos pré-antrais normais comparado aos valores do controle, exceto em solução salina a 20ºC por 4 h ou em solução à base de água de coco a 20ºC por 4 h e 12 h. Em conclusão, esse estudo mostrou que ambas as soluções podem ser usadas com igual eficiência para conservar folículos pré-antrais caprinos a 4°C, independente do tempo de incubação. No entanto, para conservar folículos pré-antrais caprinos a altas temperaturas...