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Cytotoxic and genotoxic effects of two medicinal species of Verbenaceae

SOUSA, Saulo M.; SILVA, Pamela S.; CAMPOS, Jose Marcello S.; VICCINI, Lyderson F.
Fonte: UNIV FLORENCE BOTANY INST Publicador: UNIV FLORENCE BOTANY INST
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
27.58%
Lantana camara L. and Lippia alba (Mill.) N. E. Br. are two important species of Verbenaceae family and are commonly used in folk medicine in many countries of Central and Southern America. The aim of this study was to investigate, for the first time, the cytotoxic and genotoxic effects of aqueous extracts from leaves of both species on Lactuca sativa (lettuce) root tip meristem cells using a cytogenetic approach. Seeds of lettuce were separately treated during 72h with different concentrations of L. camara and L. alba aqueous extracts (5, 10, 20 and 30g/L). The percentage of germination, root development and cellular behavior were analyzed and the results showed that the highest concentration of aqueous extracts reduced the mitotic index, the seeds germination and the root development of lettuce. The extracts also induced chromosome aberrations and cellular death in roots cells of L. sativa. The cytogenotoxicity of L. camara and L. alba extracts was comparatively described.; FAPEMIG (Fundacao de Amparo a Pesquisa do Estado de Minas Gerais); CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior); CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)

Citogenotoxicidade e determinação de metais, PAHs e organoclorados em tecidos de Cathorops spixii e Centropomus parallelus provenientes de três complexos estuarinos da costa brasileira; Citogenotoxicity and evaluation of metals, PAHs and organochloride in tissues of Cathorops spixii and Centropomus parallelus from three estuarine systems in brazilian coast

Kirschbaum, Aline Alves
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 22/10/2010 PT
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Muitos dos poluentes lançados diariamente no ambiente apresentam capacidade de bioacumulação e potencial altamente tóxico, induzindo danos em diversos níveis de organização. O peixe demersal Cathorops spixii e o pelágico Centropomus parallelus se alimentam de invertebrados bentônicos e pequenos peixes e são importantes itens alimentares para a população em geral. Neste estudo foram avaliadas as respostas citogenotóxicas através da análise de micronúcleos (MN) e anomalias nucleares (AN) em eritrócitos e a bioacumulação de PAHs e organoclorados (em fígado) e metais (em tecido muscular) em 207 exemplares dessas espécies, coletados nos Complexos Estuarinos de Cananéia, Paranaguá e Santos/São Vicente durante o inverno/08 e verão/09. Os tecidos foram extraídos para análises químicas. Na maioria dos casos, os níveis de danos citogenotóxicos obtidos nos exemplares de Paranaguá se colocaram de maneira intermediária, enquanto os da região de Santos estiveram acima dos obtidos nas outras áreas. Ficou evidenciado que os níveis de contaminação destes estuários são capazes de causar danos moleculares nos organismos. C. parallelus respondeu de maneira mais efetiva às análises dos biomarcadores utilizados. A bioacumulação refletiu principalmente a influência de fatores intrínsecos aos organismos e apresentou correlação com os dados citogenotóxicos. A ocorrência de metais e PAHs sobressaíram aos outros compostos.; Several pollutants that are daily discharged in environment are able to bioaccumulate in organism tissues and present highly toxic potential inducing damages in different levels of biological organization. The demersal Cathorops spixii catfish and the pelagic snooks Centropomus parallelus feeds on small fishes and benthic invertebrates and represent an important food source for local populations. In this study the cytogenotoxic responses in erythrocytes were evaluated by micronucleus (MN) and nuclear abnormalities (AN) analysis...

Cytogenotoxicity biomarkers in fat snook Centropomus parallelus from Cananeia and Sao Vicente estuaries, SP, Brazil

Kirschbaum, Aline A.; Seriani, Robson; Pereira, Camilo D. S.; Assuncao, Andrea; Abessa, Denis Moledo de Souza; Rotundo, Matheus M.; Ranzani-Paiva, Maria J. T.
Fonte: Soc Brasil Genetica Publicador: Soc Brasil Genetica
Tipo: Artigo de Revista Científica Formato: 151-154
ENG
Relevância na Pesquisa
27.58%
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); The aquatic environment receives many contaminants that can induce damages at the molecular, biochemical, cellular and physiological levels. Centropomus parallelus, an important food resource for local populations, is a predator fish that feeds on small fishes and benthic invertebrates, thus being vulnerable to the bioconcentration and biomagnification processes. This study aimed to evaluate cytogenotoxic responses in erythrocytes from C. parallelus juveniles collected in the Cananeia and Sao Vicente estuaries, both in winter and in summer. After anesthesia, blood samples were collected by caudal puncture. Blood smears were prepared on glass slides and stained with May-Grunwald-Giemsa dye. Two thousand cells were analyzed per slide (1000x), and nuclear abnormalities (NA) and micronuclei (MN) were scored. The Sao Vicente sample showed MN and NA frequencies (%/1000 cells) of 0.325 and 3.575, in winter, and of 0.125 and 2.935 in summer respectively; the Cananeia sample showed frequencies of 0.0325 and 0.03, in winter, and of 0.065 and 0.355 in summer, respectively. The rates found in Sao Vicente were significantly higher than those found in Cananeia, evidencing that the levels of pollution in that estuary were high enough to induce genetic damages.

Cytogenotoxicity of Cymbopogon citratus (DC) Stapf (lemon grass) aqueous extracts in vegetal test systems

Sousa,Saulo M.; Silva,Pâmela S.; Viccini,Lyderson F.
Fonte: Academia Brasileira de Ciências Publicador: Academia Brasileira de Ciências
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2010 EN
Relevância na Pesquisa
16.87%
The lemon grass, Cymbopogon citratus (DC) Stapf, is an important species of Poaceae family commonly used in the folk medicine in many countries. The aim of this study was to investigate the cytotoxic and genotoxic effects of aqueous extracts from C. citratus leaves on Lactuca sativa (lettuce) root tip meristem cells by cytogenetic studies that have never been done before for lemon grass extracts. For this, lettuce seeds were treated for 72h with different concentrations of lemon grass aqueous extracts (5; 10; 20 and 30 mg/mL). The percentage of germination, root development and cellular behavior were analyzed, and the results showed that the highest concentration of aqueous extracts reduced the mitotic index, the seed germination and the root development of lettuce. The extracts have also induced chromosome aberrations and cellular death in the roots cells of L. sativa.

Cytogenotoxicity biomarkers in fat snook Centropomus parallelus from Cananéia and São Vicente estuaries, SP, Brazil

Kirschbaum,Aline A.; Seriani,Robson; Pereira,Camilo D. S.; Assunção,Andrea; Abessa,Denis M. de Souza; Rotundo,Matheus M.; Ranzani-Paiva,Maria J. T.
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2009 EN
Relevância na Pesquisa
27.58%
The aquatic environment receives many contaminants that can induce damages at the molecular, biochemical, cellular and physiological levels. Centropomus parallelus , an important food resource for local populations, is a predator fish that feeds on small fishes and benthic invertebrates, thus being vulnerable to the bioconcentration and biomagnification processes. This study aimed to evaluate cytogenotoxic responses in erythrocytes from C. parallelus juveniles collected in the Cananéia and São Vicente estuaries, both in winter and in summer. After anesthesia, blood samples were collected by caudal puncture. Blood smears were prepared on glass slides and stained with May-Grünwald-Giemsa dye. Two thousand cells were analyzed per slide (1000x), and nuclear abnormalities (NA) and micronuclei (MN) were scored. The São Vicente sample showed MN and NA frequencies (%/1000 cells) of 0.325 and 3.575, in winter, and of 0.125 and 2.935 in summer respectively; the Cananéia sample showed frequencies of 0.0325 and 0.03, in winter, and of 0.065 and 0.355 in summer, respectively. The rates found in São Vicente were significantly higher than those found in Cananéia, evidencing that the levels of pollution in that estuary were high enough to induce genetic damages.

Cytogenotoxicity biomarkers in fat snook Centropomus parallelus from Cananéia and São Vicente estuaries, SP, Brazil

Kirschbaum, Aline A.; Seriani, Robson; Pereira, Camilo D. S.; Assunção, Andrea; de Souza Abessa, Denis M.; Rotundo, Matheus M.; Ranzani-Paiva, Maria J. T.
Fonte: Sociedade Brasileira de Genética Publicador: Sociedade Brasileira de Genética
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
16.87%
The aquatic environment receives many contaminants that can induce damages at the molecular, biochemical, cellular and physiological levels. Centropomus parallelus, an important food resource for local populations, is a predator fish that feeds on small fishes and benthic invertebrates, thus being vulnerable to the bioconcentration and biomagnification processes. This study aimed to evaluate cytogenotoxic responses in erythrocytes from C. parallelus juveniles collected in the Cananéia and São Vicente estuaries, both in winter and in summer. After anesthesia, blood samples were collected by caudal puncture. Blood smears were prepared on glass slides and stained with May-Grünwald-Giemsa dye. Two thousand cells were analyzed per slide (1000x), and nuclear abnormalities (NA) and micronuclei (MN) were scored. The São Vicente sample showed MN and NA frequencies (%/1000 cells) of 0.325 and 3.575, in winter, and of 0.125 and 2.935 in summer respectively; the Cananéia sample showed frequencies of 0.0325 and 0.03, in winter, and of 0.065 and 0.355 in summer, respectively. The rates found in São Vicente were significantly higher than those found in Cananéia, evidencing that the levels of pollution in that estuary were high enough to induce genetic damages.

Ameliorative Effects of Dimetylthiourea and N-Acetylcysteine on Nanoparticles Induced Cyto-Genotoxicity in Human Lung Cancer Cells-A549

Srivastava, Ritesh Kumar; Rahman, Qamar; Kashyap, Mahendra Pratap; Lohani, Mohtashim; Pant, Aditya Bhushan
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 29/09/2011 EN
Relevância na Pesquisa
17.94%
We study the ameliorative potential of dimetylthiourea (DMTU), an OH• radical trapper and N-acetylcysteine (NAC), a glutathione precursor/H2O2 scavenger against titanium dioxide nanoparticles (TiO2-NPs) and multi-walled carbon nanotubes (MWCNTs) induced cyto-genotoxicity in cultured human lung cancer cells-A549. Cytogenotoxicity was induced by exposing the cells to selected concentrations (10 and 50 µg/ml) of either of TiO2-NPs or MWCNTs for 24 h. Anti-cytogenotoxicity effects of DMTU and NAC were studied in two groups, i.e., treatment of 30 minutes prior to toxic insult (short term exposure), while the other group received DMTU and NAC treatment during nanoparticles exposure, i.e., 24 h (long term exposure). Investigations were carried out for cell viability, generation of reactive oxygen species (ROS), micronuclei (MN), and expression of markers of oxidative stress (HSP27, CYP2E1), genotoxicity (P53) and CYP2E1 dependent n- nitrosodimethylamine-demethylase (NDMA-d) activity. In general, the treatment of both DMTU and NAC was found to be effective significantly against TiO2-NPs and MWCNTs induced cytogenotoxicity in A549 cells. Long-term treatment of DMTU and NAC during toxic insults has shown better prevention than short-term pretreatment. Although...

Exposure of Allium cepa Root Cells to Zidovudine or Nevirapine Induces Cytogenotoxic Changes

Onwuamah, Chika K.; Ekama, Sabdat O.; Audu, Rosemary A.; Ezechi, Oliver C.; Poirier, Miriam C.; Odeigah, Peter G C.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 05/03/2014 EN
Relevância na Pesquisa
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Antiretroviral drugs have proved useful in the clinical management of HIV-infected persons, though there are concerns about the effects of exposure to these DNA-reactive drugs. We investigated the potential of the plant model Allium cepa root tip assay to demonstrate the cytogenotoxicity of zidovudine and nevirapine and as a replace-reduce-refine programme amenable to resource–poor research settings. Cells mitotic index were determined in squashed root cells from Allium cepa bulbs exposed to zidovudine or nevirapine for 48 hr. The concentration of zidovudine and nevirapine inhibiting 50% root growth after 96 hr exposure was 65.0 µM and 92.5 µM respectively. Root length of all antiretroviral-exposed roots after 96 hr exposure was significantly shorter than the unexposed roots while additional root growth during a subsequent 48 hr recovery period in the absence of drug was not significantly different. By ANOVA, there was a significant association between percentage of cells in mitosis and zidovudine dose (p = 0.004), but not nevirapine dose (p = 0.68). Chromosomal aberrations such as sticky chromosomes, chromatin bridges, multipolar mitoses and binucleated cells were observed in root cells exposed to zidovudine and nevirapine for 48 hr. The most notable chromosomal aberration was drug-related increases in sticky chromosomes. Overall...

Differences in Cytotoxic, Genotoxic, and Inflammatory Response of Bronchial and Alveolar Human Lung Epithelial Cells to Pristine and COOH-Functionalized Multiwalled Carbon Nanotubes

Ursini, Cinzia Lucia; Cavallo, Delia; Fresegna, Anna Maria; Ciervo, Aureliano; Maiello, Raffaele; Buresti, Giuliana; Casciardi, Stefano; Bellucci, Stefano; Iavicoli, Sergio
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
16.87%
Functionalized MWCNTs are used in many commercial and biomedical applications, but their potential health effects are not well defined. We investigated and compared cytotoxic, genotoxic/oxidative, and inflammatory effects of pristine and carboxyl MWCNTs exposing human respiratory (A549 and BEAS-2B) cells to 1–40 μg/mL of CNTs for 24 h. Both MWCNTs induced low viability reduction (by WST1 assay) in A549 cells and only MWCNTs-COOH caused high viability reduction in BEAS-2B cells reaching 28.5% viability at 40 μg/mL. Both CNTs induced membrane damage (by LDH assay) with higher effects in BEAS-2B cells at the highest concentrations reaching 20% cytotoxicity at 40 μg/mL. DNA damage (by Fpg-comet assay) was induced by pristine MWCNTs in A549 cells and by both MWCNTs in BEAS-2B cells reaching for MWCNTs-COOH a tail moment of 22.2 at 40 μg/mL versus 10.2 of unexposed cells. Increases of IL-6 and IL-8 release (by ELISA) were detected in A549 cells exposed to MWCNTs-COOH from 10 μg/mL while IL-8 increased in BEAS-2B cells exposed to pristine MWCNTs at 20 and 40 μg/mL. The results show higher cytogenotoxicity of MWCNTs-COOH in bronchial and of pristine MWCNTs in alveolar cells. Different inflammatory response was also found. The findings suggest the use of in vitro models with different end points and cells to study CNT toxicity.

Citogenética e efeitos citogenotóxicos de Annona crassiflora Mart. (Annonaceae)

Fonte: UNIVERSIDADE FEDERAL DE LAVRAS; DBI - Programa de Pós-graduação; UFLA; BRASIL Publicador: UNIVERSIDADE FEDERAL DE LAVRAS; DBI - Programa de Pós-graduação; UFLA; BRASIL
Tipo: Tese de Doutorado
PT_BR
Relevância na Pesquisa
16.87%
Tese apresentada à Universidade Federal de Lavras, como parte das exigências do Programa de Pós-Graduação em Genética e Melhoramento de Plantas, área de concentração em Genética e Melhoramento de Plantas, para a obtenção do título de Doutor.; Fundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIG

Mecanismos anatômicos, citológicos e fisiológicos de vetiver (Chrysopogon zizanioides (L.) Roberty) para a recuperação de ambientes aquáticos contaminados por cádmio e chumbo

Fonte: UNIVERSIDADE FEDERAL DE LAVRAS; DBI - Programa de Pós-graduação; UFLA; BRASIL Publicador: UNIVERSIDADE FEDERAL DE LAVRAS; DBI - Programa de Pós-graduação; UFLA; BRASIL
Tipo: Artigo de Revista Científica
PT_BR
Relevância na Pesquisa
16.87%
Tese apresentada à Universidade Federal de Lavras, como parte das exigências do Programa de Pós-Graduação em Agronomia/Fisiologia Vegetal área de concentração em Anatomia Vegetal, para a obtenção do título de “Doutor”.; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES

Investigação citogenética do potencial alelopático de espécies de líquens e pteridófitas; Cytogenetic investigation of the allelopathic potential of lichens and pteridophytas species.

Fonte: UNIVERSIDADE FEDERAL DE LAVRAS; DBI - Departamento de Biologia; UFLA; BRASIL Publicador: UNIVERSIDADE FEDERAL DE LAVRAS; DBI - Departamento de Biologia; UFLA; BRASIL
Tipo: Dissertação
PT_BR
Relevância na Pesquisa
16.87%

In vitro mucus transportability, cytogenotoxicity, and hematological changes as non-destructive physiological biomarkers in fish chronically exposed to metals

Seriani, Robson; Abessa, Denis M. S.; Moreira, Lucas B.; Cabrera, Joana P. G.; Sanches, Juliana Q.; Silva, Carolina L. S.; Amorim, Francisca A.; Rivero, Dolores H. R. F.; Silva, Flavia L.; Fitorra, Lilian S.; Carvalho-Oliveira, Regiani; Macchione, Mariang
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 162-168
ENG
Relevância na Pesquisa
16.87%
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); The biomonitoring of fish using biomarkers represents a useful tool for the assessment of aquatic pollution. This study evaluated the sublethal toxic effects of aquatic pollution on fish collected from a site contaminated by metals. Water and fish (Oreochromis niloticus) samples were collected from a pond in the Parque Ecologico do Tiete (PET) that lies along the Tiete River (Sao Paulo, Brazil), and from a control site (an experimental fish farm). The metal content of the water was evaluated, and fish were used to examine the properties of gill mucus and blood. The PET fish were evaluated for alterations in the in vitro transportability of mucus and changes in blood properties (e.g., cell volume, hemoglobin concentration, red blood cells, and white blood cell count). The results of the water analyzes indicated metal levels above the legal standards for Fe (0.71 mg/L), Ni (0.06 mg/L), Mn (0.11 mg/L), and Pb (0.48 mg/L). Compared to the controls, the hematologic parameter analyzes of PET fish revealed significantly higher numbers of erythrocytes (RBC), leukocytes (WBC), lymphocytes, erythroblasts, and Mean Corpuscular Volume (MCV); however, the hemoglobin content and Mean Corpuscular Hemoglobin Concentration (MCHC) values were significantly lower. The frequencies of nuclear abnormalities and micronuclei were significantly higher and the mucociliary transport was significantly lower in PET fish than in the controls. These results suggest that fish from the contaminated site exhibit a series of physiological responses...

Hematological changes and cytogenotoxicity in the tilapia Oreochromis niloticus caused by sub-chronic exposures to mercury and selenium

Seriani, Robson; Franca, Jakeline Galvão; Lombardi, Julio Vicente; Brito, Jôse Mara; Tavares Ranzani-Paiva, Maria José
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica Formato: 311-322
ENG
Relevância na Pesquisa
27.94%
Fish bioassays are valuable tools that can be used to elucidate the toxicological potential of numerous substances that are present in the aquatic environment. In this study, we assessed the antagonistic action of selenium (Se) against the toxicity of mercury (Hg) in fish (Oreochromis niloticus). Six experimental groups with six fish each were defined as follows: (1) control, (2) mercury (HgCl2), (3) sodium selenite (Na2Se4O3), (4) sodium selenate (Na2Se6O4), (5) mercury + sodium selenite (HgCl2 + Na2Se4O3), and (6) mercury + sodium selenate (HgCl2 + Na2Se6O4). Hematological parameters [red blood cells (RBC), white blood cells (WBC), and erythroblasts (ERB)] in combination with cytogenotoxicity biomarkers [nuclear abnormalities (NAs) and micronuclei (MN)] were examined after three, seven, ten, and fourteen days. After 7 days of exposure, cytogenotoxic effects and increased erythroblasts caused by mercury, leukocytosis triggered by mercury + sodium selenite, leukopenia associated with sodium selenate, and anemia triggered by mercury + sodium selenate were observed. Positive correlations that were independent of time were observed between WBC and RBC, ERB and MN, and NA and MN. The results suggest that short-term exposure to chemical contaminants elicited changes in blood parameters and produced cytogenotoxic effects. Moreover...

Cytogenotoxicity of SPL "(Spent Pot Liner)" by plant root tip bioassays; Citogenotoxidade do SPL (Spent Pot Liner) por meio de bioensaios vegetais em ápice radicular

Andrade, Larissa Fonseca
Fonte: UNIVERSIDADE FEDERAL DE LAVRAS; DBI - Departamento de Biologia; UFLA; BRASIL Publicador: UNIVERSIDADE FEDERAL DE LAVRAS; DBI - Departamento de Biologia; UFLA; BRASIL
Tipo: Dissertação
Publicado em 07/10/2014 EN
Relevância na Pesquisa
16.87%
O SPL (Spent Pot Liner) é um resíduo sólido, tóxico, gerado nas indústrias de alumínio, durante o processo de eletrólise da alumina. Sua composição é bastante variável, incluindo sais de fluoreto, cianetos, metais e compostos orgânicos. No Brasil, mais de 35.000 t de SPL são geradas por ano. Algumas indústrias já adotaram o co-processamento do produto, que é utilizado como matéria prima nas indústrias cimenteiras. No entanto, os efeitos do SPL sobre os sistemas biológicos necessitam ser compreendidos, o que poderia auxiliar nas estratégias que buscam solucionar tal problema mais diretamente. A utilização de plantas para estudos de mutagênese tem sido reconhecida como excelente indicadora de alterações genéticas provocadas pela presença de substâncias químicas no meio ambiente e uma enorme quantidade de poluentes ambientais já foi testada. Neste contexto, metodologias que evidenciam danos no material genético, ou em estruturas celulares e tecidos são ferramentas úteis no estudo de citotoxidade de compostos sobre organismos. Além disso, as raízes são comumente utilizadas nos testes biológicos, pois são as primeiras a serem expostas aos produtos químicos ou resíduos lançados no solo ou na água. O objetivo do presente trabalho foi avaliar o efeito citogenotóxico do SPL em modelos biológicos por meio de bioensaios vegetais. Os resultados evidenciaram o efeito tóxico do SPL em função da alta freqüência de alterações cromossômicas...

Óleos essenciais de três espécies de myrtaceae:composição química, atividades antioxidante, hemolítica, antitumoral, antiocratoxigênica e citogenotóxica

Santiago, Juliana de Andrade
Fonte: Universidade Federal de Lavras; Programa de Pós-Graduação em Agroquímica; UFLA; brasil; Departamento de Química Publicador: Universidade Federal de Lavras; Programa de Pós-Graduação em Agroquímica; UFLA; brasil; Departamento de Química
Tipo: Tese de Doutorado
Publicado em 17/12/2015 POR
Relevância na Pesquisa
16.87%
The aim of this study was to determine the chemical composition and various biological activities of the essential oils from Melaleuca alternifolia (leaves and fruits), Melaleuca quinquenervia (leaves and fruits), and Backhousia citriodora (leaves). The EOs were extracted through the hydrodistillation technique and chemically characterized by GC/MS and GC-FID. The antitumor tests were performed in five cell lines derived from human tumors: A549, MCF7, HepG2, U251-MG and HT144. The frequency of apoptotic cells was analyzed by fluorescence microscopy; morphological changes through contrast microscopy, and DNA content, in the different phases of the cell cycle, through flow cytometry. In addition, several tests were performed to evaluate the antioxidant activity (ABTS, DPPH, hydroxyl radical, β-carotene/ linoleic acid, TBARS , and phosphomolybdenum complex). Hemolytic activity was evaluated in liquid medium (0.15% Ht) and solid medium. The oils extracted from the leaves were evaluated for inhibition of ochratoxin production by Aspergillus niger and A. carbonarius under two different conditions, 15 to 25°C. The cytogenotoxic effects of the essential oil (EO) from B. citriodora were evaluated on Lactuca sativa, using cytogenetic assays...