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Staphylococcus Aureus Contamination in a Pediatric Dental Clinic

NEGRINI, Thais de Cassia; DUQUE, Cristiane; OLIVEIRA, Ana Carolina Mascarenhas de; HEBLING, Josimeri; SPOLIDORIO, Luis C.; SPOLIDORIO, Denise M. P.
Fonte: JOURNAL PEDODONTICS INC Publicador: JOURNAL PEDODONTICS INC
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
46.2%
Staphylococcus aureus strains can be disseminated during dental treatment and occasionally lead to contamination and infection of patients and dentists. The objective of this study was to determine the frequency and compare the number of S.aureus colonies isolated from the nose, hands and tongue of students and patients, as well as from the clinical environment, before and after dental treatment. Staphylococcus species were isolated from the tongue, nose and hands of 30 students and 30 patients and from the environment of a Pediatric Dentistry Clinic. The samples were incubated in SMA plates at 37 degrees C for 48 hours. Results: The colonies that showed the presence of mannitol fermentation were collected as identification for Staphylococcus aureus, using CHROMagar and the coagulase test. The highest amount of S.aureus was found in the nose and tongue of children. In relation to dental students, more contamination was observed on gloved hands, followed by the tongue and hands without gloves, before clinical attendance. At the end of dental treatment, S. aureus colonies isolated from the gloved hands of students decreased significantly. Considering the clinical environment, the most contaminated areas were the auxiliary table and the storeroom...

Contaminação microbiológica e avaliação de métodos de higienização de panos de limpeza utilizados em serviços de alimentação; Microbial contamination and evaluation of two disinfection methods of cleaning cloths used in food services

Bartz, Sabrina
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
56.19%
Panos de limpeza têm sido considerados importantes fontes de contaminação cruzada, contudo seu uso continua muito freqüente em serviços de alimentação. O objetivo desse trabalho foi avaliar a contaminação e multiplicação microbiana, além de dois procedimentos de higienização de panos de limpeza. Em uma primeira etapa, 35 panos de limpeza foram coletados em serviços de alimentação da grande Porto Alegre, RS/Brasil e foram submetidos à quantificação de bactérias totais, coliformes e Staphylococcus coagulase positiva, aqui chamado de Staphylococcus aureus presuntivos. Os panos foram lavados manualmente e desinfetados por dois métodos, separadamente: a) fervura em água potável por 15 minutos e b) imersão em solução clorada a 200ppm, por 15 minutos, sendo enxaguados logo após. Os resultados demonstraram que a as contagens de bactérias totais variaram de 2,0 x 104 UFC/cm2 até 1,0 x 108 UFC/cm2, com média de 9,1 x 106 UFC/cm2. A contaminação por coliformes foi de 4,4 x 102 a 1,6 x 107 UFC/cm2, sendo que 40% das amostras apresentou contagens de aproximadamente 106 UFC/cm2. Quantidades de S. aureus presuntivos variaram de 1,0 x 104 UFC/cm2 a 2,8 x 106 UFC/cm2, com média de 4,6 x 105 UFC/cm2. De modo geral...

Avaliação da contaminação microbiológica e de procedimentos de desinfecção de esponjas utilizadas em serviços de alimentação; Evaluation of microbiological contamination and disinfection procedures of sponges used in food services

Rossi, Eliandra Mirlei
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
POR
Relevância na Pesquisa
56.15%
Esponjas de cozinha podem promover contaminação cruzada ao transferirem microrganismos de superfícies variadas para os alimentos. Os objetivos deste estudo foram avaliar a contaminação microbiológica e a eficácia de dois procedimentos de desinfecção de esponjas utilizadas em serviços de alimentação, bem como avaliar a transferência microbiana a partir de esponjas para o aço inoxidável e polietileno. Na primeira parte deste estudo, 80 esponjas naturalmente contaminadas foram coletadas em serviços de alimentação e então transferidas para o laboratório, onde foram divididas em três partes iguais. Uma das partes foi submetida à contagem de microrganismos heterotróficos (MH), coliformes a 45 oC (CF), Staphylococcus coagulase positiva (SA) e à pesquisa de Salmonella sp. (SAM). As outras duas partes foram submetidas, separadamente, à fervura em água durante cinco minutos e à desinfecção por hipoclorito de sódio 200ppm, por 10 minutos, adicionada de enxágue com água potável. Na segunda parte do estudo, 24 esponjas naturalmente contaminadas foram friccionadas sobre superfícies de aço inoxidável AISI 316 e polietileno, separadamente, a fim de investigar o número de microrganismos transferidos e sua sobrevivência. Os resultados demonstraram contaminações médias por MH de aproximadamente 9...

Transfer of Salmonella Enteritidis to four types of surfaces after cleaning procedures and cross-contamination to tomatoes

Soares, Vanessa Mendonca; Pereira, Juliano Goncalves; Viana, Cibeli; Izidoro, Thiago Braga; Bersot, Luciano dos Santos; Pinto, José Paes de Almeida Nogueira
Fonte: Academic Press Ltd Elsevier B.V. Ltd Publicador: Academic Press Ltd Elsevier B.V. Ltd
Tipo: Artigo de Revista Científica Formato: 453-456
ENG
Relevância na Pesquisa
66.17%
The objectives of the present study were to evaluate the spread of Salmonella Enteritidis to different cutting boards (wood, triclosan-treated plastic, glass, and stainless steel) from contaminated poultry skin (5 log CFU/g) and then to tomatoes and to analyze the effect of different protocols used to clean these surfaces to control contamination. The following procedures were simulated: (1) no cleaning after handling contaminated poultry skin; (2) rinsing in running water; (3) cleaning with dish soap and mechanical scrubbing; and (4) cleaning with dish soap and mechanical scrubbing, followed by disinfection with hypochlorite. The pathogen was recovered from all surfaces following procedure 1, with counts ranging from 1.90 to 2.80 log, as well as from the tomatoes handled on it. Reduced numbers of S. Enteritidis were recovered using the other procedures, both from the surfaces and from the tomatoes. Counts were undetectable after procedure 4. From all surfaces evaluated, wood was the most difficult to clean, and stainless steel was the easiest. The use of hypochlorite as a disinfecting agent helped to reduce cross-contamination. (C) 2011 Elsevier Ltd. All rights reserved.

Cross-contamination in the dental laboratory through the polishing procedure of complete dentures

Agostinho,Alessandra Marçal; Miyoshi,Paula Regina; Gnoatto,Nelson; Paranhos,Helena de Freitas Oliveira; Figueiredo,Luciene Cristina de; Salvador,Sérgio Luiz
Fonte: Fundação Odontológica de Ribeirão Preto Publicador: Fundação Odontológica de Ribeirão Preto
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2004 EN
Relevância na Pesquisa
56.17%
Polishing of dental prostheses can cause a dangerous cycle of cross-contamination involving dentists, laboratory technicians, patients and auxiliary personnel. The aim of this study was to show the microbial contamination in the dental laboratory during the polishing procedure of complete dentures. For this purpose, 4 experiments were conducted. Experiment I - Determination of the total colony-forming units (CFU) counts contaminating complete maxillary dentures. During the polishing procedure, determination of the CFU counts transferred to the operator (Experiment II) and of the total CFU counts transferred to previously sterilized complete dentures (Experiment III). Experiment IV - The total counts of remaining CFU in the lathe spindle after Experiments II and III. Complete dentures were highly contaminated (mean = 1.4 x 10(7) CFU/mL). There was a elevated level of contamination by splatter and aerosols. There was high microbial transfer from the contaminated lathe spindle to the sterile prostheses (mean = 1.7 x 10(7) CFU/mL). The spindles were highly contaminated after polishing procedures (mean = 3.5 x 10(8) CFU/mL). The polishing of dental prostheses is a possible source of transmission of communicable diseases in the laboratory and requires improved techniques for infection control.

Estimation of the Rate of Unrecognized Cross-Contamination with Mycobacterium tuberculosis in London Microbiology Laboratories

Ruddy, M.; McHugh, T. D.; Dale, J. W.; Banerjee, D.; Maguire, H.; Wilson, P.; Drobniewski, F.; Butcher, P.; Gillespie, S. H.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /11/2002 EN
Relevância na Pesquisa
46.26%
Isolates from patients with confirmed tuberculosis from London were collected over 2.5 years between 1995 and 1997. Restriction fragment length polymorphism (RFLP) analysis was performed by the international standard technique as part of a multicenter epidemiological study. A total of 2,779 samples representing 2,500 individual patients from 56 laboratories were examined. Analysis of these samples revealed a laboratory cross-contamination rate of between 0.54%, when only presumed cases of cross-contamination were considered, and 0.93%, when presumed and possible cases were counted. Previous studies suggest an extremely wide range of laboratory cross-contamination rates of between 0.1 and 65%. These data indicate that laboratory cross-contamination has not been a common problem in routine practice in the London area, but in several incidents patients did receive full courses of therapy that were probably unnecessary.

Inoculum Size Influences Bacterial Cross Contamination between Surfaces

Montville, R.; Schaffner, D. W.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /12/2003 EN
Relevância na Pesquisa
46.24%
Many factors have been shown to influence bacterial transfer between surfaces, including surface type, bacterial species, moisture level, pressure, and friction, but the effect of inoculum size on bacterial transfer has not yet been established. Bacterial cross contamination rates during performance of common food service tasks were previously determined in our laboratory using nalidixic acid-resistant Enterobacter aerogenes. Eight different transfer rates were determined, each involving a minimum of 30 volunteers. The influence of source inoculum level on the percentage of bacteria transferred (percent transfer rates) and log10 CFU per recipient surface was determined using statistical analysis. The effect of inoculum size on transfer rate was highly statistically significant (P < 0.0001) for all transfer rate data combined (352 observations) and for each individual cross contamination rate, except for data on contamination via transfer from chicken to hand through a glove barrier (P = 0.1643). Where inoculum size on the source was greater, transfer rates were lower, and where inoculum size on the source was less, transfer rates were higher. The negative linear trend was more obvious for activities that had a larger range of inoculum sizes on the source surface. This phenomenon has serious implications for research seeking to determine bacterial cross contamination rates...

Cross contamination of blood cultures associated with a multiple use venting device.

Holliman, R E; Johnson, J D
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /03/1999 EN
Relevância na Pesquisa
46.35%
AIM: To investigate cross contamination of blood cultures associated with multiple use venting devices, which are widely used in clinical microbiology laboratories to reduce labour costs. METHODS: Systematic analysis of 13,880 blood culture results in a large teaching hospital where multiple use venting devices were employed. RESULTS: Nine series of potential cross contamination were identified in a 12 month period. Four series involved coagulase negative staphylococci and were unlikely to represent true cross contamination. Five series involved blood cultures which had significant bacterial growth at the time of venting. CONCLUSIONS: Multiple use venting devices can be associated with cross contamination of blood cultures. This may result from contamination of the internal lumen of the venting device which is not exposed to the biocide. Medical microbiologists should consider the possibility of cross contamination associated with venting procedures when interpreting blood culture results. Further development of multiple use venting devices is required to reduce the risk of cross contamination of cultures.

Evidence of cross-contamination by Campylobacter spp. of broiler carcasses using genetic characterization of isolates

Normand, Valérie; Boulianne, Martine; Quessy, Sylvain
Fonte: Canadian Veterinary Medical Association Publicador: Canadian Veterinary Medical Association
Tipo: Artigo de Revista Científica
Publicado em /10/2008 EN
Relevância na Pesquisa
46.2%
Campylobacter is recognized as one of the leading cause of gastroenteritis worldwide, and is frequently isolated from the small intestines and ceca microflora of chickens. Twenty-one out of 81 Campylobacter-positive poultry flocks were selected to evaluate the genetic diversity of Campylobacter isolates and to study the distribution of genotypes among flocks. Campylobacter isolates recovered from chicken carcasses and ceca were analyzed by pulsed-field gel electrophoresis (PFGE). Little diversity was found among Campylobacter strains isolated from a given carcass, with a maximum of 2 different genotypes being present. However, at flock level, as many as 4 different profiles were observed. Typing of strains showed that most strains isolated from ceca were similar to those isolated from corresponding broiler carcasses. A total of 39 different macrorestriction profiles were observed, with evidence of Campylobacter cross-contamination among broiler flocks in Quebec slaughterhouses. Surprisingly, some flocks shared related genotypes both with and without sharing similar rearing practices. Existence of such cross-contamination must be considered to in developing strategies to control Campylobacter in chickens, and to avoid bacteria contamination of noncolonized flocks. Further typing studies of Campylobacter found in hatcheries...

Factors Associated with Cross-Contamination of Hides of Scottish Cattle by Escherichia coli O157▿

Mather, A. E.; Reid, S. W. J.; McEwen, S. A.; Ternent, H. E.; Reid-Smith, R. J.; Boerlin, P.; Taylor, D. J.; Steele, W. B.; Gunn, G. J.; Mellor, D. J.
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.29%
The putative source of hide contamination for 236 cattle in Scotland followed from the farm through to slaughter was determined using phage and verocytotoxin type data. The majority of cattle (84%) were found to have subtypes of Escherichia coli O157 on their hide that had not been found previously in any animal from the farm of origin, strongly suggesting that contamination occurred once animals had left the farm of origin. Using logistic regression analysis, several variables and factors were found to be strongly associated (P < 0.01) with cross-contamination of cattle hides at the univariate level; commercial transport to slaughter, transport with other animals, use of a crush, line automation, and increasing slaughterhouse throughput were all risk factors, while feeding hay in lairage, processing an animal earlier in a slaughter cohort, and cleaning the landing area poststunning were protective. In the multivariable model, with the slaughterhouse and the farm group included as random effects, factors associated with the cross-contamination of cattle hides were identified. Transport to the slaughterhouse by a commercial hauler had a borderline-significant association with increased odds of an animal having a cross-contaminated hide (odds ratio [OR] [95% confidence interval {CI}] = 5.7 [0.99...

Evaluation of Low-Colony-Number Counts of Mycobacterium tuberculosis on Solid Media as a Microbiological Marker of Cross-Contamination▿

Ribeiro, F. K. C.; Lemos, E. M.; Hadad, D. J.; Leão, S. C.; Viana-Niero, C.; Dietze, R.; Johnson, J. L.; Eisenach, K. D.; Palaci, M.
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.2%
Low-colony-number counts on solid media are considered characteristic of cross-contamination, although they are normally observed in true-positive cultures from some groups of patients. The aim of this study was to evaluate low-yield growth cultures as a microbiological marker for cross-contamination. We evaluated 106 cultures with <15 colonies from 94 patients, and the proportions of false-positive cultures were 0.9% per sample and 1.1% per patient, which indicates that low-yield growth is not a reliable marker of cross-contamination.

A Prospective, Multicenter Study of Laboratory Cross-Contamination of Mycobacterium tuberculosis Cultures

Jasmer, Robert M.; Roemer, Marguerite; Hamilton, John; Bunter, John; Braden, Christopher R.; Shinnick, Thomas M.; Desmond, Edward P.
Fonte: Centers for Disease Control and Prevention Publicador: Centers for Disease Control and Prevention
Tipo: Artigo de Revista Científica
Publicado em /11/2002 EN
Relevância na Pesquisa
46.2%
A prospective study of false-positive cultures of Mycobacterium tuberculosis that resulted from laboratory cross-contamination was conducted at three laboratories in California. Laboratory cross-contamination accounted for 2% of the positive cultures. Cross-contamination should be a concern when an isolate matches the genotype of another sample processed during the same period.

Infrequent MODS TB culture cross-contamination in a high-burden resource-poor setting

Moore, David A.J.; Caviedes, Luz; Gilman, Robert H.; Coronel, Jorge; Arenas, Fanny; LaChira, Doris; Salazar, Cayo; Saravia, Juan Carlos; Oberhelman, Richard A.; Hollm-Delgado, Maria-Graciela; Escombe, A. Roderick; Evans, Carlton A.W.; Friedland, Jon S.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.3%
One obstacle to wider use of rapid liquid culture-based tuberculosis diagnostics such as the microscopic observation drug susceptibility (MODS) assay is concern about cross-contamination. We investigated the rate of laboratory cross-contamination in MODS, automated MBBacT, and Lowenstein–Jensen (LJ) cultures performed in parallel, through triangulation of microbiologic (reculturing stored samples), molecular (spoligotype/RFLP), and clinical epidemiologic data. At least 1 culture was positive for Mycobacterium tuberculosis for 362 (11%) of 3416 samples; 53 were regarded as potential cross-contamination suspects. Cross-contamination accounted for 17 false-positive cultures from 14 samples representing 0.41% (14/3416) and 0.17% (17/10 248) of samples and cultures, respectively. Positive predictive values for MODS, MBBacT (bioMérieux, Durham, NC), and LJ were 99.1%, 98.7%, and 99.7%, and specificity was 99.9% for all 3. Low rates of cross-contamination are achievable in mycobacterial laboratories in resource-poor settings even when a large proportion of samples are infectious and highly sensitive liquid culture-based diagnostics such as MODS are used.

Identity tests: determination of cell line cross-contamination

Cabrera, C. M.; Cobo, F.; Nieto, A.; Cortés, J. L.; Montes, R. M.; Catalina, P.; Concha, A.
Fonte: Kluwer Academic Publishers Publicador: Kluwer Academic Publishers
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.26%
Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses.

Laser Cutting Eliminates Nucleic Acid Cross-Contamination in Dried-Blood-Spot Processing

Murphy, Sean C.; Daza, Glenda; Chang, Ming; Coombs, Robert
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /12/2012 EN
Relevância na Pesquisa
46.2%
Dried blood spots (DBS) are useful for molecular assays but are prone to false positives from cross-contamination. In our malaria DBS assay, cross-contamination was encountered despite cleaning techniques suitable for HIV-1. We therefore developed a contact-free laser cutting system that effectively eliminated cross-contamination during DBS processing.

Effect of Disinfectants on Preventing the Cross-Contamination of Pathogens in Fresh Produce Washing Water

Banach, Jennifer L.; Sampers, Imca; Van Haute, Sam; van der Fels-Klerx, H.J. (Ine)
Fonte: MDPI Publicador: MDPI
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
46.2%
The potential cross-contamination of pathogens between clean and contaminated produce in the washing tank is highly dependent on the water quality. Process wash water disinfectants are applied to maintain the water quality during processing. The review examines the efficacy of process wash water disinfectants during produce processing with the aim to prevent cross-contamination of pathogens. Process wash water disinfection requires short contact times so microorganisms are rapidly inactivated. Free chlorine, chlorine dioxide, ozone, and peracetic acid were considered suitable disinfectants. A disinfectant’s reactivity with the organic matter will determine the disinfectant residual, which is of paramount importance for microbial inactivation and should be monitored in situ. Furthermore, the chemical and worker safety, and the legislative framework will determine the suitability of a disinfection technique. Current research often focuses on produce decontamination and to a lesser extent on preventing cross-contamination. Further research on a sanitizer’s efficacy in the washing water is recommended at the laboratory scale, in particular with experimental designs reflecting industrial conditions. Validation on the industrial scale is warranted to better understand the overall effects of a sanitizer.

Surveillance and cross contamination of salmonella spp., in pork, with profiles of nalidixic acid resistance in salmonella

Dillon, Colm
Fonte: University of Limerick Publicador: University of Limerick
Tipo: info:eu-repo/semantics/masterThesis; all_ul_research; ul_published_reviewed; ul_theses_dissertations
ENG
Relevância na Pesquisa
66.26%
peer-reviewed; Salmonella is a major foodborne pathogen and porcine products are an important source. With this in mind, pork sausages were surveyed for Salmonella prevalence. Sausages were sampled during August-December 2008, none of which were positive for Salmonella. As an alternative porcine source of salmonella, 102 pig ear pet treats were surveyed from October 2008 to September 2009. Salmonella was detected in 24.5% of treats using a culture detection method and 28.4% using peR. As dogs are the main consumers of pig ear treats, a veterinary surveillance study was undertaken. Salmonella was not detected in any of 86 rectal swabs from dogs attending 2 different veterinary clinics between February and April and August and October 2009. Emergence of antimicrobial resistance is a significant health problem. Nalidixic Acid resistance is seen as a marker for the emergence of Fluoroquinolone resistance, the antimicrobials of choice for treating salmonella infections. Nalidixic Acid intermediate (4 isolates) and resistant (5 isolates) isolates were examined for mutations in the Quinolone Resistance Determining Region (QRDR) of gyrA and pare. Single mutations at Serine-83 and Aspaltic Acid-87 in the QRDR of gyrA were observed for all Nalidixic Acid resistant isolates. One intermediate isolate contained a mutation in parC...

Establecimiento de un sistema para el monitoreo y control de la contaminación cruzada en el laboratorio de análisis microbiológico de alimentos durante 2009; Establishment of a System for Monitoring and Control of Cross Contamination in the Laboratory of Microbiological Analysis of Food during 2009; Estabelecimento de um sistema para a monitoração e controle da contaminação cruzada no laboratório de análise microbiológico de alimentos durante 2009

Corpas Iguarán, Eduardo Javid
Fonte: Universidade do Rosário Publicador: Universidade do Rosário
Tipo: Artigo de Revista Científica Formato: application/pdf
Publicado em 30/04/2012 SPA
Relevância na Pesquisa
66.26%
Dentro de las actividades para el control de calidad en el laboratorio, los resultados finales de un analito en particular son considerados productos intermedios, dada la pertinencia otorgada al aseguramiento de la calidad como fin último de los programas de gestión de la calidad. Esta concepción precisa el establecimiento de instrumentos integrales para la detección de eventos como la contaminación cruzada y la adopción de medidas para evitar que se afecte la marcha analítica. Objetivo: el objetivo principal fue establecer un sistema para el monitoreo y control de la contaminación cruzada en el laboratorio de análisis microbiológico de alimentos. Materiales y métodos: la metodología empleada consistió en desarrollar diagramas de flujo para los procedimientos sobre el control de las poblaciones de mesófilos aerobios y mohos provenientes de la contaminación en los ambientes, superficies, material estéril y medios de cultivos. Dichos diagramas incluyeron un árbol de decisiones, diseñado para efectuar acciones de control con base en los intervalos de tolerancia, establecidos como herramienta objetiva hacia la toma de decisiones que normalicen los recuentos de las poblaciones microbianas en cuestión. Resultados: los límites de alerta más estrictos se obtuvieron para las poblaciones de mesófilos aerobios y mohos en los diferentes controles...

Cross-contamination during the preparation of frozen chickens in the kitchen.

de Wit, J. C.; Broekhuizen, G.; Kampelmacher, E. H.
Fonte: Cambridge University Press Publicador: Cambridge University Press
Tipo: Artigo de Revista Científica
Publicado em /08/1979 EN
Relevância na Pesquisa
46.2%
A study was made of the extent to which frozen broilers, contaminated with indicator organisms, can cause cross-contamination in the kitchen. In 60 kitchens a number of relevant objects were sampled during the preparation of contaminated frozen broilers. The results show that cross-contamination occurred in a high proportion of the kitchens examined. In many instances the indicator organism was still present on various objects even after rinsing, 'clearing' or washing up. In view of the possible risk of a cross-contamination with Salmonella spp. the importance of instructing food preparers is emphasized. No salmonellas could be found in the sinks of the 60 kitchens examined.

Impact of Wash Water Quality on Sensory and Microbial Quality, Including Escherichia coli Cross-Contamination, of Fresh-Cut Escarole

Allende, Ana; Selma, María Victoria; López-Gálvez, Francisco; Villaescusa, Raquel; Gil Muñoz, M.ª Isabel
Fonte: International Association for Food Protection Publicador: International Association for Food Protection
Tipo: Artículo Formato: 259768 bytes; application/pdf
ENG
Relevância na Pesquisa
46.34%
5 pages.; The influence of wash water quality on the microbial load and sensory quality of fresh-cut escarole was evaluated. Additionally, the degree of Escherichia coli cross-contamination between inoculated and uninoculated products after washing was also studied. Three types of wash water, i.e., potable water, diluted recirculated water, and recirculated water, containing different microbial counts and organic loads, were used. Results showed that microbial load (P ≥ 0.02) and sensory quality (P > 0.625) of the product were not influenced by the water quality after washing and storage. Cross-contamination between inoculated and uninoculated products was observed after washing, as there was significant transmission of E. coli cells from the product to the wash water (P < 0.001). When fresh-cut escarole was contaminated at a high inoculum level (5.1 log CFU/g), wash water quality influenced the level of cross-contamination, as the highest E. coli load (P < 0.001) was shown in uninoculated fresh-cut escarole washed with recirculated water. However, when fresh-cut escarole was contaminated at a low inoculum level (3.2 log CFU/g), the wash water quality did not influence the level of cross-contamination, as E. coli slightly increased...