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Cloning, characterization and functional analysis of a 1-FEH cDNA from Vernonia herbacea (Vell.) Rusby

ASEGA, Amanda Francine; NASCIMENTO, Joao Roberto O. do; SCHROEVEN, Lindsey; ENDE, Wim Van den; CARVALHO, Maria Angela M.
Fonte: OXFORD UNIV PRESS Publicador: OXFORD UNIV PRESS
Tipo: Artigo de Revista Científica
ENG
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36.46%
Variations in the inulin contents have been detected in rhizophores of Vernonia herbacea during the phenological cycle. These variations indicate the occurrence of active inulin synthesis and depolymerization throughout the cycle and a role for this carbohydrate as a reserve compound. 1-Fructan exohydrolase (1-FEH) is the enzyme responsible for inulin depolymerization, and its activity has been detected in rhizophores of sprouting plants. Defoliation and low temperature are enhancer conditions of this 1-FEH activity. The aim of the present work was the cloning of this enzyme. Rhizophores were collected from plants induced to sprout, followed by storage at 5C. A full length 1-FEH cDNA sequence was obtained by PCR and inverse PCR techniques, and expressed in Pichia pastoris. Cold storage enhances FEH gene expression. Vh1-FEH was shown to be a functional 1-FEH, hydrolyzing predominantly -2,1 linkages, sharing high identity with chicory FEH sequences, and its activity was inhibited by 81 in the presence of 10 mM sucrose. In V. herbacea, low temperature and sucrose play a role in the control of fructan degradation. This is the first study concerning the cloning and functional analysis of a 1-FEH cDNA of a native species from the Brazilian Cerrado. Results will contribute to understanding the role of fructans in the establishment of a very successful fructan flora of the Brazilian Cerrado...

alpha-Hydroxynitrile lyase protein from Xylella fastidiosa: Cloning, expression, and characterization

CARUSO, Celia Sulzbacher; TRAVENSOLO, Regiane de Fatima; BICUDO, Rogerio de Campus; LEMOS, Eliana Gertrudes de Macedo; ARAUJO, Ana Paula Ulian de; CARRILHO, Emanuel
Fonte: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD Publicador: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Tipo: Artigo de Revista Científica
ENG
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Xylella fastidiosa is a xylem-restricted plant pathogen that causes a range of diseases in several and important crops. Through comparative genomic sequence analysis many genes were identified and, among them, several potentially involved in plant-pathogen interaction. The experimental determination of the primary sequence of some markedly expressed proteins for X fastidiosa and the comparison with the nucleic acids sequence of genome identified one of them as being SCJ21.16 (XFa0032) gene product. The comparative analysis of this protein against SWISSPROT database, in special, resulted in similarity with a-hydroxynitrile lyase enzyme (HNL) from Arabidopsis thaliana, causing interest for being one of the most abundant proteins both in the whole cell extract as well as in the extracellular protein fraction. It is known that HNL enzyme are involved in a process termed ""cyanogenesis"", which catalyzes the dissociation of alpha-hydroxinitrile into carbonyle and HCN when plant tissue is damaged. Although the complete genome sequences of X.fastidiosa are available and the cyanogenesis process is well known, the biological role of this protein in this organism is not yet functionally characterized. In this study we presented the cloning...

O uso da cardiotocografia como método de diagnóstico da ocorrência de sofrimento fetal (hipóxia fetal) durante a vida intrauterina de fetos da raça Nelore originados por meio da técnica de transferência nuclear de células somáticas adultas - Clonagem; The use of cardiotocography as a method of diagnosis of the occurrence of fetal distress (fetal hypoxia) during intrauterine life in fetuses of Nellore generated through the technique of somatic adult cell nuclear transfer - Cloning

Nunes, Mariana Tikuma
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 28/08/2009 PT
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A presente pesquisa teve a finalidade de padronizar a técnica de cardiotocografia a ser utilizada para avaliar a existência de hipóxia/sofrimento fetal durante a vida intrauterina, procurando estabelecer a partir do 7º mês da gestação os padrões de normalidade da frequência cardíaca fetal para fetos sadios e oriundos de inseminação artificial, bem como avaliar a influência da clonagem nos parâmetros obtidos no exame de cardiotocografia. Foram acompanhadas as gestações de 14 vacas, sendo os animais divididos em três grupos experimentais: Grupo de Clones Mortos composto de 5 vacas gestantes nas quais acompanhou-se a carditocografia de fetos gerados pela técnica de clonagem e nos quais a morte do bezerro ocorreu nas primeiras 36 horas de vida ; Grupo de Clones Vivos composto de 4 vacas gestantes nas quais acompanhou-se a carditocografia de fetos gerados pela técnica de clonagem e que permaneceram vivos após o nascimento; Grupo Controle - composto de 5 vacas gestantes nas quais acompanhou-se a carditocografia de fetos gerados por inseminação artificial e que deram origem a bezerros sadios. Os animais foram acompanhados por exames periódicos de cardiotocografia realizados nos seguintes momentos: 90, 60, 30, 15, 7...

Aspectos morfofisiológicos na clonagem de Eucalyptus benthamii; Morphophysiological aspects in the cloning of Eucalyptus benthamii

Brondani, Gilvano Ebling
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 18/05/2012 PT
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Dentre as poucas espécies de Eucalyptus que apresentam aptidão ao cultivo em regiões de baixas temperaturas e a geadas frequentes, destacam-se genótipos de Eucalyptus benthamii que representam opções para futuros plantios florestais em diferentes regiões brasileiras. Porém, existem poucas informações quanto a obtenção de mudas clonais, e se focarmos as espécies aconselhadas para o plantio em condições subtropicais, tal carência é ainda maior, principalmente ao considerar os fatores endógenos e exógenos para o enraizamento adventício. Baseado no exposto, o presente trabalho teve como objetivo geral avaliar aspectos morfofisiológicos da clonagem de genótipos de Eucalyptus benthamii por meio das técnicas de miniestaquia e micropropagação. Para tanto, o trabalho foi dividido em quatro estudos básicos. O primeiro estudo (Capítulo 2) baseou-se na avaliação da morfofisiologia de um minijardim clonal em relação a diferentes concentrações de Zn e B ao longo de sucessivas coletas de brotações. O segundo estudo (Capítulo 3) foi baseado em avaliar o enraizamento de miniestacas quanto a diferentes concentrações de Zn e B e aplicação de AIB ao longo de sucessivas coletas de brotações. O terceiro estudo (Capítulo 4) baseou-se em avaliar a dinâmica de enraizamento de miniestacas quanto a diferentes concentrações de AIB...

Clonagem, expressão e purificação da quinase dependente de ciclina 10 (CDK10) humana; Cloning, expression and purification of human cyclin dependente kinase 10 (CDK10)

Lamas, Cíntia Betite
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 12/09/2014 PT
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36.46%
Quinases dependentes de ciclinas (CDKs) compreendem uma família de proteínas que podem ser subdivididas em dois grupos funcionais majoritários baseados na sua função no ciclo celular e/ou controle transcricional. Já foram identificadas mais de 30 CDKs humanas. A CDK10 é uma proteína quinase dependente de ciclina pertencente ao grupo de quinases relacionadas à Cdc2. CDK10 é essencial na fase G2/M do ciclo celular, possivelmente no progresso dessa fase, monitorando a replicação completa do DNA e permitindo que as células passem desse ponto de restrição. Essa proteína é um importante determinante de resistência à terapia endócrina para câncer de mama. Portanto, é um alvo potencial para o desenvolvimento de inibidores, uma vez que está presente em células cancerosas. O estudo da estrutura e função da CDK10 deverá ser realizado após sua clonagem, expressão e purificação. O cDNA da CDK10 foi amplificado por reação em cadeia da polimerase (PCR), e posteriormente, o produto foi aplicado em gel de agarose para análise e purificação. O vetor de clonagem recombinante foi obtido, o qual foi clonado em células competentes e sequenciado. A obtenção do plasmídeo recombinante para expressão deu-se pela inserção do DNA nos vetores de expressão pET28a(+) e pET23a(+) (Novagen). A proteína foi expressa em células competentes e analisada por eletroforese em gel de poliacrilamida. Em seguida...

Avaliação de sistemas de cultivo in vitro em micropoços para embriões bovinos produzidos por handmade cloning (HMC)

Feltrin, Cristiano
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Dissertação Formato: application/pdf
POR
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36.53%
Sistemas de produção in vitro de embriões mamíferos muitas vezes requerem que o cultivo embrionário seja realizado de forma individualizada. Entretanto, os resultados obtidos com o cultivo in vitro (CIV) individual são inconstantes e, por vezes, inferiores ao CIV em grupo. Entre os sistemas que requerem o CIV individual, a técnica de handmade cloning (HMC) se destaca por produzir embriões sem zona pelúcida que não podem ser cultivados agrupados em protocolos convencionais de CIV. O objetivo deste experimento foi determinar as taxas de desenvolvimento in vitro e in vivo de embriões bovinos clonados pela técnica de HMC e submetidos a três diferentes sistemas de CIV em micropoços (Well of the well – WOW), sendo um industrial, confeccionado em polidimetilsiloxano (PDMS), que visou à padronização da configuração do sistema de CIV. Após 11 replicações, de 3.876 complexos cumuli-oócito bovinos maturados in vitro, 3.437 (88,6%) oócitos apresentaram a extrusão do 1º corpúsculo polar. Após a digestão da zona pelúcida, 2.992 estruturas foram bisseccionadas manualmente, com a produção de 2.288 hemi-citoplastos. Reconstruiram-se 1.011 embriões pela adesão de dois hemi-citoplastos a uma célula somática (célula-tronco mesenquimal bovina de uma fêmea adulta da raça Nelore)...

alpha-Hydroxynitrile lyase protein from Xylella fastidiosa: Cloning, expression, and characterization

Caruso, Celia Sulzbacher; Travensolo, Regiane de Fatima; Bicudo, Rogerio de Campus; de Macedo Lemos, Eliana Gertrudes; Ulian de Araujo, Ana Paula; Carrilho, Emanuel
Fonte: Academic Press Ltd Elsevier B.V. Ltd Publicador: Academic Press Ltd Elsevier B.V. Ltd
Tipo: Artigo de Revista Científica Formato: 118-127
ENG
Relevância na Pesquisa
36.46%
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Xylella fastidiosa is a xylem-restricted plant pathogen that causes a range of diseases in several and important crops. Through comparative genomic sequence analysis many genes were identified and, among them, several potentially involved in plant-pathogen interaction. The experimental determination of the primary sequence of some markedly expressed proteins for X fastidiosa and the comparison with the nucleic acids sequence of genome identified one of them as being SCJ21.16 (XFa0032) gene product. The comparative analysis of this protein against SWISSPROT database, in special, resulted in similarity with a-hydroxynitrile lyase enzyme (HNL) from Arabidopsis thaliana, causing interest for being one of the most abundant proteins both in the whole cell extract as well as in the extracellular protein fraction. It is known that HNL enzyme are involved in a process termed "cyanogenesis", which catalyzes the dissociation of alpha-hydroxinitrile into carbonyle and HCN when plant tissue is damaged. Although the complete genome sequences of X.fastidiosa are available and the cyanogenesis process is well known...

Cloning of glucocorticoid-regulated genes in C6/ST1 rat glioma phenotypic reversion

Valentini, S. R.; Armelin, M. C. S.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 11-17
ENG
Relevância na Pesquisa
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The C6 rat glioma cell line is responsive to glucocorticoid hormones. C6 variants that are hyper-responsive (ST1) and resistant (P7) to hormone treatment have been derived previously. Glucocorticoid treatment of ST1 cells leads to complete reversion of the transformed phenotype and loss of tumorigenic potential. Production of C type retrovirus particles is also induced by glucocorticoids in ST1 cells. Cloning of the genes regulated by glucocorticoids in this cell system was used here as a strategy to uncover the gene products involved in the transformed-to-normal phenotypic change. Construction of a cDNA library from glucocorticoid-treated ST1 cells and screening by differential hybridization resulted in the isolation of three cellular sequences that code for rat metallothioneins (C27 and C41) and α1-acid glycoprotein (C36). Northern blot analysis revealed that expression of these genes was dramatically induced by hydrocortisone in ST1 but not in P7 cells. Viral genomic RNA was used to isolate and characterize retrovirus-related sequences that could also be responsible for the phenotypic reversion phenomenon.

Hyaluronidase from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae): Cloning, structural modeling, purification, and immunological analysis

Justo Jacomini, Débora Laís; Campos Pereira, Franco Dani; Aparecido dos Santos Pinto, José Roberto; dos Santos, Lucilene Delazari; da Silva Neto, Antonio Joaquim; Giratto, Danielli Thieza; Palma, Mario Sergio; de Lima Zollner, Ricardo; Brochetto Braga,
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 70-80
ENG
Relevância na Pesquisa
36.53%
In this study, we describe the cDNA cloning, sequencing, and 3-D structure of the allergen hyaluronidase from Polybia paulista venom (Pp-Hyal). Using a proteomic approach, the native form of Pp-Hyal was purified to homogeneity and used to produce a Pp-specific polyclonal antibody. The results revealed that Pp-Hyal can be classified as a glycosyl hydrolase and that the full-length Pp-Hyal cDNA (1315 bp; GI: 302201582) is similar (80-90%) to hyaluronidase from the venoms of endemic Northern wasp species. The isolated mature protein is comprised of 338 amino acids, with a theoretical pI of 8.77 and a molecular mass of 39,648.8 Da versus a pI of 8.13 and 43,277.0 Da indicated by MS. The Pp-Hyal 3D-structural model revealed a central core (α/β)7 barrel, two sulfide bonds (Cys 19-308 and Cys 185-197), and three putative glycosylation sites (Asn79, Asn187, and Asn325), two of which are also found in the rVes v 2 protein. Based on the model, residues Ser299, Asp107, and Glu109 interact with the substrate and potential epitopes (five conformational and seven linear) located at surface-exposed regions of the structure. Purified native Pp-Hyal showed high similarity (97%) with hyaluronidase from Polistes annularis venom (Q9U6V9). Immunoblotting analysis confirmed the specificity of the Pp-Hyal-specific antibody as it recognized the Pp-Hyal protein in both the purified fraction and P. paulista crude venom. No reaction was observed with the venoms of Apis mellifera...

Cloning goes to the movies

Cormick,Craig
Fonte: Casa de Oswaldo Cruz, Fundação Oswaldo Cruz Publicador: Casa de Oswaldo Cruz, Fundação Oswaldo Cruz
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2006 EN
Relevância na Pesquisa
36.62%
Public attitude research conducted by Biotechnology Australia shows that one of the major sources of information on human reproductive cloning is movies. Traditionally, understanding of new and emerging technologies has come through the mass media but human cloning, being so widely addressed through the popular culture of movies, is more effectively defined by Hollywood than the news media or science media. But how well are the science and social issues of cloning portrayed in box office hits such as The Island, Multiplicity, Star Wars: Attack of the Clones and Jurassic Park? These movies have enormous reach and undoubted influence, and are therefore worth analyzing in some detail. This study looks at 33 movies made between 1971 and 2005 that address human reproductive cloning, and it categorizes the films based on their genre and potential influence. Yet rather than simply rating the quality of the science portrayed, the study compares the key messages in these movies with public attitudes towards cloning, to examine the correlations.

Protection of genetic heritage in the era of cloning

Oliveira Júnior,Eudes Quintino de; Oliveira,Pedro Bellentani Quintino de
Fonte: Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular Publicador: Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2012 EN
Relevância na Pesquisa
36.58%
Research on human beings has expanded greatly due to progress and the evolution of society as well as customs. Not only the unceasing development of research on human beings, but also interference in the beginning and end of life with homologous and heterogonous human reproduction, surrogate motherhood, cloning, gene therapies, eugenics,euthanasia, dysthanasia, orthothanasia, assisted suicide, genetic engineering, reassignment surgery in cases of transsexuality, the use of recombinant DNA technology and embryonic stem cells, transplantation of human organs and tissues, biotechnology and many other scientific advances. Scientific progress goes faster than the real needs of human beings, who are the final recipient of the entire evolutionary progress. Hence, there is the need to scrutinize whether new technologies are necessary, suitable and timely so that humanity can achieve its postulate of bene vivere. Human cloning, as an abrupt scientific fact, has presented itself to the world community as a procedure that can be performed with relative success and with little difficulty, since it achieved its objectives with the cloning of Dolly the sheep. This issue became the topic of discussion not only in the scientific community but in the lay population...

The Historical Development of Cloning Technology and the Role of Regulation in Ensuring Responsible Applications

Paul, Darcy A.
Fonte: Harvard University Publicador: Harvard University
Tipo: Paper (for course/seminar/workshop)
EN_US
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36.62%
Recent developments in biotechnology have led to significant advances in the ability to clone mammals. Although the state of scientific understanding has not reached the point where the cloning of a human being from a fully differentiated adult cell is possible, multiple groups have either made the attempt or asserted that they have broken this biological barrier. At this point, for reasons of safety and efficacy, these attempts must be strictly regulated, and even banned. An examination of the historical development of this technology reveals why cloning techniques were able to advance to such a refined state before being regulated and how regulatory mechanisms have addressed recent attempts to apply cloning techniques to human genetic material. For the time being, these stop-gap measures may be sufficient, but will be unlikely to provide a permanent solution in the longer term. When the technology progresses to the point where human cloning is reasonably safe, regulation will need to speak to underlying substantive questions involving our conceptions of sentience and life.

Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations

Underwood, B.; Vanderhaeghen, R.; Whitford, R.; Town, C.; Hilson, P.
Fonte: Blackwell Publishing Ltd. Publicador: Blackwell Publishing Ltd.
Tipo: Artigo de Revista Científica
Publicado em //2006 EN
Relevância na Pesquisa
36.62%
Comprehensive open reading frame (ORF) clone collections, ORFeomes, are key components of functional genomics projects. When recombinational cloning systems are used to capture ORFs in master clones, these DNA sequences can be easily transferred into a variety of expression plasmids, each designed for a specific assay. Depending on downstream applications, an ORF is cloned either with or without a stop codon at its original position, referred to as closed or open configuration, respectively. The former is preferred when the encoded protein is produced in its native form or with an amino-terminal tag; the latter is obligatory when the protein is produced as a fusion with a carboxyl-terminal tag. We developed a streamlined protocol for high-throughput, simultaneous cloning of both open and closed ORF entry clones with the Gateway recombinational cloning system. The protocol is straightforward to set up in large-scale ORF cloning projects, and is cost-effective, because the initial ORF amplification and the cloning in a pDONR vector are performed only once to obtain the two ORF configurations. We illustrated its implementation for the isolation and validation of 346 Arabidopsis ORF entry clones.; http://www.ncbi.nlm.nih.gov/pubmed/17147637; Underwood...

Data cloning estimation of GARCH and COGARCH models

Marín, J. Miguel; Rodríguez Bernal, M. T.; Romero, Eva
Fonte: Universidade Carlos III de Madrid Publicador: Universidade Carlos III de Madrid
Tipo: info:eu-repo/semantics/draft; info:eu-repo/semantics/workingPaper Formato: application/pdf
Publicado em /07/2013 ENG
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GARCH models include most of the stylized facts of financial time series and they have been largely used to analyze discrete financial time series. In the last years, continuous time models based on discrete GARCH models have been also proposed to deal with non-equally spaced observations, as COGARCH model based on Lévy processes. In this paper, we propose to use the data cloning methodology in order to obtain estimators of GARCH and COGARCH model parameters. Data cloning methodology uses a Bayesian approach to obtain approximate maximum likelihood estimators avoiding numerically maximization of the pseudo-likelihood function. After a simulation study for both GARCH and COGARCH models using data cloning, we apply this technique to model the behavior of some NASDAQ time series

Molecular cloning and restriction endonuclease analysis of bovine adenovirus type 3

Elgadi, Mabrouk Mohammed.
Fonte: Brock University Publicador: Brock University
Tipo: Electronic Thesis or Dissertation
ENG
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Bovine adenovirus type 3 (BAV3) is a medium size DNA virus that causes respiratory and gastrointestinal disorders in cattle. The viral genome consists of a 35,000 base pair, linear, double-stranded DNA molecule with inverted terminal repeats and a 55 kilodalton protein covalently linked to each of the 5' ends. In this study, the viral genome was cloned in the form of subgenomic restriction fragments. Five EcoRI internal fragments spanning 3.4 to 89.0 % and two Xb a I internal fragments spanning 35.7 to 82.9 % of the viral genome were cloned into the EcoRI and Xbal sites of the bacterial vector pUC19. To generate overlap between cloned fragments, ten Hi n dIll internal fragments spanning 3.9 to 84.9 and 85.5 to 96% and two BAV3 BamHI internal fragments spanning 59.8 to 84.9% of the viral genome were cloned into the HindllI and BamHI sites of pUC19. The HindlII cloning strategy also resulted in six recombinant plasmids carrying two or more Hi ndII I fragments. These fragments provided valuable information on the linear orientation of the cloned fragments within the viral genome. Cloning of the terminal fragments required the removal of the residual peptides that remain attached to the 5' ends of the genome. This was accomplished by alkaline hydrolysis of the DNA-peptide bond. BamH I restriction fragments of the peptide-free DNA were cloned into pUC19 and resulted in two plasmids carrying the BAV3 Bam HI terminal fragments spanning 0 to 53.9% and 84.9 to 100% of the viral genome.; Bovine adenovirus type 3 (BAV3) is a medium size DNA virus that causes respiratory and gastrointestinal disorders in cattle. The viral genome consists of a 35...

Borrowing Trouble: Should the FDA Regulate Human Cloning?

Carmody, Allison R.
Fonte: Harvard University Publicador: Harvard University
Tipo: Paper (for course/seminar/workshop)
EN_US
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Less than a year after scientist Ian Wilmut announced the birth of Dolly, the world's first cloned sheep, entrepreneur and physicist Richard Seed stated on National Public Radio that he intended to establish a for-profit clinic to clone human beings as soon as the technology was available. An immediate, visceral reaction to the prospect of human cloning reverberated throughout the nation and the rest of the world, as private and public organizations alike rushed to impose moratoriums, pass legislation, and appeal to scientists' morality to suppress any attempts to clone a human being. In the thick of this debate it became apparent that no existing arm of the federal government had jurisdiction to monitor privately-funded research. The Food and Drug Administration (FDA) then stepped forward and asserted that it, in fact, did have authority to regulate human cloning under the Public Health Service Act and the Federal Food, Drug and Cosmetic Act. Since then the dust has settled. More than one legal scholar has questioned the FDA's claim to authority, and the FDA itself concedes to be re-evaluating its position. While legal scholars struggle to define the scope of the FDA's power, ethical scholars appear engaged in a debate over the moral implications of cloning a human. What these groups have failed to address satisfactorily...

The Dolly case, the Polly drug, and the morality of human cloning

Schramm,Fermin Roland
Fonte: Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz Publicador: Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/1999 EN
Relevância na Pesquisa
36.53%
The year 1996 witnessed the cloning of the lamb Dolly, based on the revolutionary somatic cell nuclear transfer (SCNT) technique, developed by researchers from the Roslin Institute in Edinburgh, Scotland. This fact marked a relevant biotechnoscientific innovation, with probable significant consequences in the field of public health, since in principle it allows for expanding possibilities for the reproductive autonomy of infertile couples and carriers of diseases of mitochondrial origin. This article expounds on 1) the experiment's technical data and the theoretical implications for the biological sciences; 2) the public's perception thereof and the main international documents aimed at the legal and moral regulation of the technique; and 3) the moral arguments for and against cloning, from the point of view of consequentialist moral theory. We conclude that in the current stage of the debate on the morality of cloning, in which there are no cogent deontological arguments either for or against, weighing the probability of risks and benefits is the only reasonable way of dealing with the issue in societies that consider themselves democratic, pluralistic, and tolerant

Cloning: usurpation of God or exercise of the capacity He has given to us?; Clonagem: usurpação de Deus ou exercício da capacidade que ele nos conferiu?

Almeida, Marcos de
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ART.; Formato: application/pdf
Publicado em 07/11/2003 POR
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After making a brief story of cloning in the world the author establishes a sort of classification of cloning, sugests the steps to be taken and summarizes some barriers to be overtaken. After that makes some reflexions on the issue and uses some arguments founded on practical reasons to deffend therapeutic cloning. He ends by saying that any absolute prohibition is morally unjustified, puts upside down the conception of dignity, besides being a theological heresy. The aparent strenght of the impulse to banish cloning, seems to come from a tremendous nostalgia some people possess by the Middle Ages.; Após historiar de modo breve a clonagem no mundo, o autor estabelece a classificação dos quatro tipos de clonagem e lista os degraus a serem galgados e os obstáculos a serem superados. Em seguida procede a uma serie de reflexões e utiliza argumentos fundamentados na razão prática, para defender a clonagem terapêutica. Conclui por afirmar que qualquer proibição absoluta é moralmente injustificada, subverte o conceito de dignidade, além de ser teologicamente uma heresia. A aparente força do impulso para banir a clonagem, em qualquer de suas formas, para vir de uma enorme nostalgia que alguns sentem pela Inquisição.

Principais aspectos legais e constitucionais da clonagem reprodutiva humana; Human reproductive cloning: main legal and constitutional aspects

Glina, Nathan
Fonte: Universidade de São Paulo. Faculdade de Medicina Publicador: Universidade de São Paulo. Faculdade de Medicina
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 07/11/2005 POR
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This study aims to analyze the most important aspects of the human reproductive cloning, from the view point of the Brazilian Federal Constitution of 1988. To achieve the purposed aim, the research and analysis of the recent legal and constitutional aspects which can be related to human reproductive cloning has been performed.; O objetivo do estudo é delinear os principais aspectos da clonagem humana reprodutiva, sob a ótica da Constituição Federal Brasileira de 1988. Para atingir esse objetivo, foram realizadas a pesquisa e análise relativa aos aspectos constitucionais e da legislação atual que podem ser aplicados à clonagem humana reprodutiva.

The Dolly case, the Polly drug, and the morality of human cloning

Schramm,Fermin Roland
Fonte: Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz Publicador: Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/1999 EN
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The year 1996 witnessed the cloning of the lamb Dolly, based on the revolutionary somatic cell nuclear transfer (SCNT) technique, developed by researchers from the Roslin Institute in Edinburgh, Scotland. This fact marked a relevant biotechnoscientific innovation, with probable significant consequences in the field of public health, since in principle it allows for expanding possibilities for the reproductive autonomy of infertile couples and carriers of diseases of mitochondrial origin. This article expounds on 1) the experiment's technical data and the theoretical implications for the biological sciences; 2) the public's perception thereof and the main international documents aimed at the legal and moral regulation of the technique; and 3) the moral arguments for and against cloning, from the point of view of consequentialist moral theory. We conclude that in the current stage of the debate on the morality of cloning, in which there are no cogent deontological arguments either for or against, weighing the probability of risks and benefits is the only reasonable way of dealing with the issue in societies that consider themselves democratic, pluralistic, and tolerant