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Avaliação da ultraestrutura e do movimento ciliar em crianças com pneumopatias crônicas e de repetição se diagnóstico definido; Evaluation of the ultrastructure and of the movement of cilia in children with chronic and repetition pneumopathies without a defined diagnosis

Olm, Mary Anne Kowal
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 05/03/2010 PT
Relevância na Pesquisa
37.32%
INTRODUÇÃO: A discinesia ciliar primária é uma doença genética que se caracteriza pela alteração da ultraestrutura e função do cílio móvel, com consequentes alterações do transporte mucociliar, causando infecções das vias aéreas superiores, inferiores e infertilidade. O diagnóstico, realizado por avaliação da ultraestrutura ou pesquisa de mutação genética, é feito mediante critérios de seleção de pacientes e testes de screening. Esta pesquisa avalia a ultraestrutura e frequência de batimento ciliar, propõe um modelo de investigação de discinesia ciliar primária, e caracteriza os pacientes diagnosticados. MÉTODO: Foi realizado um estudo transversal controlado entre janeiro de 2007 e julho de 2009, no Ambulatório de Pneumologia Pediátrica do Instituto da Criança. Foram selecionadas 28 crianças e adolescentes (6 meses a 19 anos, de ambos os sexos), de uma população de 75 crianças com pneumopatias crônicas e de repetição sem diagnóstico definido, que apresentavam ao menos um dos seguintes achados: bronquiectasia de causa desconhecida, doença de vias aéreas superiores com sintomatologia crônica, infecções pulmonares de repetição, dextrocardia e/ou situs inversus acompanhados de sintomas em vias aéreas superiores e/ou inferiores...

CCTα and CCTδ chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena

Seixas, Cecília; Cruto, Teresa; Tavares, Alexandra; Gaertig, Jacek; Soares, Helena
Fonte: PLoS Publicador: PLoS
Tipo: Artigo de Revista Científica
Publicado em /05/2010 ENG
Relevância na Pesquisa
37.15%
Background - The eukaryotic cytosolic chaperonin CCT is a hetero-oligomeric complex formed by two rings connected back-to-back, each composed of eight distinct subunits (CCTalpha to CCTzeta). CCT complex mediates the folding, of a wide range of newly synthesised proteins including tubulin (alpha, beta and gamma) and actin, as quantitatively major substrates. Methodology/Principal findings - We disrupted the genes encoding CCTalpha and CCTdelta subunits in the ciliate Tetrahymena. Cells lacking the zygotic expression of either CCTalpha or CCTdelta showed a loss of cell body microtubules, failed to assemble new cilia and died within 2 cell cycles. We also show that loss of CCT subunit activity leads to axoneme shortening and splaying of tips of axonemal microtubules. An epitope-tagged CCTalpha rescued the gene knockout phenotype and localized primarily to the tips of cilia. A mutation in CCTalpha, G346E, at a residue also present in the related protein implicated in the Bardet Biedel Syndrome, BBS6, also caused defects in cilia and impaired CCTalpha localization in cilia. Conclusions/Significance - Our results demonstrate that the CCT subunits are essential and required for ciliary assembly and maintenance of axoneme structure, especially at the tips of cilia.

Cilia motility studies in zebrafish embryos

Ferreira, Rita Joana Rodrigues da Silva Rua
Fonte: Faculdade de Ciências e Tecnologia Publicador: Faculdade de Ciências e Tecnologia
Tipo: Dissertação de Mestrado
Publicado em //2012 ENG
Relevância na Pesquisa
37.29%
A thesis submitted in fulfilment of the requirements for the degree of Masters in Molecular Genetics and Biomedicine; Motile ciliary dysfunctions cause specific Ciliopathies that affect mainly the respiratory tract, fertilization and left-right body establishment. The embryonic organ where left-right decisions are first taken is called the organizer, a ciliated organ where a leftward cilia driven fluid-flow is generated. The organizer is named node in the mouse and Kupffer’s vesicle (KV) in zebrafish. The correct left-right axis formation is highly dependent on signaling pathways downstream of such directional fluid-flow. Motile cilia need to be coordinated and Ciliary Beat Frequency (CBF) is characteristic of different types of cilia depending on their function. Using zebrafish as a model, our group has been studying cilia length regulation and motility in wild-type and deltaD-/- mutant embryos. Recently, we showed that Notch signalling was directly involved in the control of cilia length in the KV cells given that the deltaD-/- mutant present shorter KV cilia. The goal of this project was to characterize the CBF of deltaD-/- KV cilia vs. wild-type cilia and reveal how potential differences in CBF impact on KV fluid flow, using spectral analysis associated with highspeed videomicroscopy. By decomposing and comparing the obtained CBF with Fast Fourier Transform...

Using cilia mutants to study left-right asymmetry in zebrafish

Sampaio, Pedro Rafael Martins de Almeida
Fonte: Faculdade de Ciências e Tecnologia Publicador: Faculdade de Ciências e Tecnologia
Tipo: Dissertação de Mestrado
Publicado em //2014 ENG
Relevância na Pesquisa
37.27%
A thesis submitted in fulfillment of the requirements for the degree of the Masters in Molecular Genetics and Biomedicine; In vertebrates, internal organs are positioned asymmetrically across the left-right (L-R) body axis. Events determining L-R asymmetry occur during embryogenesis, and are regulated by the coordinated action of genetic mechanisms. Embryonic motile cilia are essential in this process by generating a directional fluid flow inside the zebrafish organ of asymmetry, called Kupffer’s vesicle ﴾KV). A correct L-R formation is highly dependent on signaling pathways downstream of such flow, however detailed characterization of how its dynamics modulates these mechanisms is still lacking. In this project, fluid flow measurements were achieved by a non-invasive method, in four genetic backgrounds: Wild-type (WT), deltaD-/- mutants, Dnah7 morphants (MO) and control-MO embryos. Knockdown of Dnah7, a heavy chain inner axonemal dynein, renders cilia completely immotile and depletes the KV directional fluid flow, which we characterize here for the first time. By following the development of each embryo, we show that flow dynamics in the KV is already asymmetric and provides a very good prediction of organ laterality. Through novel experiments...

Biochemical studies of olfaction: isolation, characterization, and odorant binding activity of cilia from rainbow trout olfactory rosettes.

Rhein, L D; Cagan, R H
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /08/1980 EN
Relevância na Pesquisa
27.32%
The role of cilia in recognition of olfactory stimuli has been controversial. Cilia from the intact olfactory rosettes of the rainbow trout Salmo gairdneri were isolated, characterized biochemically, and examined by electron microscopy. The markers studied are those associated with cilia in other organisms. Dynein arms contain Mg2+-AtPase; this enzyme was enriched in the isolated cilia preparation. Guanine nucleotides are associated with the outer microtubule doublets of cilia but adenine nucleotides are not; a substantial enrichment in guanine, relative to adenine, was found in the cilia preparation. Tubulin, the structural protein component of microtubules, occurs in large amounts in cilia. Disc gel electrophoresis indicated tubulin in the cilia preparation. Electron microscopy confirmed the presence of cilia in the isolated preparation. Rainbow trout have an acute sense of smell and many amino acids are odorants to this species. Functional activity of the cilia preparation relevant to odorant recognition was assessed by using binding of radioactively labeled odorant amino acids. L-Alanine, L-serine, L-threonine, L-lysine, and D-alanine bound to the cilia preparation. This study provides direct biochemical evidence that olfactory cilia bind odorant molecules and supports the hypothesis that odorant recognition sites are integral parts of the cilia.

FGF Signaling during embryo development regulates cilia length in diverse epithelia

Neugebauer, Judith M.; Amack, Jeffrey D.; Peterson, Annita G.; Bisgrove, Brent W.; Yost, H. Joseph
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.29%
Cilia are cell surface organelles found on most epithelia in vertebrates. Specialized groups of cilia play critical roles in embryonic development, including left-right (LR) axis formation. Recently, cilia have been implicated as recipients of cell-cell signaling1, 2. However, little is known about cell-cell signaling pathways that control the length of cilia3. Here we provide several lines of evidence showing that fibroblast growth factor (FGF) signaling regulates cilia length and function in diverse epithelia during zebrafish and Xenopus development. Morpholino (MO) knockdown of FGF receptor 1 (FGFR1) in zebrafish cell-autonomously reduces cilia length in Kupffer’s vesicle (KV) and perturbs directional fluid flow required for LR patterning of the embryo. Expression of a dominant-negative FGFR (DN-FGFR), treatment with SU5402, a pharmacological inhibitor of FGF signaling, or genetic and morpholino reduction of redundant FGF ligands FGF8 and FGF24, reproduces this cilia length phenotype. Knockdown of FGFR1 also results in shorter tethering cilia in the otic vesicle and shorter motile cilia in the pronephric ducts. In Xenopus, expression of a DN-FGFR results in shorter monocilia in the gastrocoel roof plate (GRP) that control LR patterning4 and in shorter multicilia in external mucociliary epithelium. Together...

Cilia movement regulates expression of the Raf-1 kinase inhibitor protein

Sas, Kelli M.; Janech, Michael G.; Favre, Elizabeth; Arthur, John M.; Bell, P. Darwin
Fonte: American Physiological Society Publicador: American Physiological Society
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
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Renal epithelial cell primary cilia act as mechanosensors in response to changes in luminal fluid flow. To determine the role of cilia bending in the mechanosensory function of cilia, we performed proteomic analysis of collecting duct cell lines with or without cilia that were kept stationary or rotated to stimulate cilia bending. Expression of the Raf-1 kinase inhibitor protein (RKIP), an inhibitor of the MAPK pathway, was significantly elevated in rotated cilia (+) cells. This was compared with RKIP levels in cilia (−) cells that were stationary or rotated as well as in cilia (+) cells that were stationary. This result was confirmed in cilia knockout adult mice that had lower renal RKIP levels compared with adult mice with cilia. Downstream of RKIP, expression of phosphorylated ERK was decreased only in cells that had cilia and were subjected to constant cilia bending. Furthermore, elevated RKIP levels were associated with reduced cell proliferation. Blockade of PKC abrogated ciliary bending-induced increases in RKIP. In summary, we found that ciliary movement may help control the expression of the Raf-1 kinase inhibitor protein and thus maintain cell differentiation. In terms of polycystic kidney disease, loss of cilia and therefore sensitivity to flow may lead to reduced RKIP levels...

Proteomic Analysis of Mammalian Primary Cilia

Ishikawa, Hiroaki; Thompson, James; Yates, John R.; Marshall, Wallace F.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.3%
The primary cilium is a microtubule-based organelle that senses extracellular signals as a cellular antenna [1]. Primary cilia are found on many types of cells in our body and play important roles in development and physiology. Defects of primary cilia cause a broad class of human genetic diseases called ciliopathies. To gain new insights into ciliary functions and better understand the molecular mechanisms underlying ciliopathies, it is of high importance to generate a catalog of primary cilia proteins. In this study, we isolated primary cilia from mouse kidney cells by using a calcium shock method and identified 195 candidate primary cilia proteins by MudPIT (multidimensional protein identification technology), protein correlation profiling, and subtractive proteomic analysis. Based on comparisons with other proteomic studies of cilia, around 75% of our candidate primary cilia proteins are shared components with motile or specialized sensory cilia. The remaining 25% of the candidate proteins are possible primary cilia specific proteins. These possible primary cilia specific proteins include Evc2, Inpp5e and Inversin, several of which have been linked to known ciliopathies. We have performed the first reported proteomic analysis of primary cilia from mammalian cells. These results provide new insights into primary cilia structure and function.

Collecting duct cells that lack normal cilia have mislocalized vasopressin-2 receptors

Saigusa, Takamitsu; Reichert, Ryan; Guare, Jennifer; Siroky, Brian J.; Gooz, Monika; Steele, Stacy; Fenton, Robert A.; Bell, P. Darwin; Kolb, Robert J.
Fonte: American Physiological Society Publicador: American Physiological Society
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.32%
Polycystic kidney disease (PKD) is a ciliopathy characterized by renal cysts and hypertension. These changes are presumably due to altered fluid and electrolyte transport in the collecting duct (CD). This is the site where vasopressin (AVP) stimulates vasopressin-2 receptor (V2R)-mediated aquaporin-2 (AQP2) insertion into the apical membrane. Since cysts frequently occur in the CD, we studied V2R and AQP2 trafficking and function in CD cell lines with stunted and normal cilia [cilia (−), cilia (+)] derived from the orpk mouse (hypomorph of the Tg737/Ift88 gene). Interestingly, only cilia (−) cells grown on culture dishes formed domes after apical AVP treatment. This observation led to our hypothesis that V2R mislocalizes to the apical membrane in the absence of a full-length cilium. Immunofluorescence indicated that AQP2 localizes to cilia and in a subapical compartment in cilia (+) cells, but AQP2 levels were elevated in both apical and basolateral membranes in cilia (−) cells after apical AVP treatment. Western blot analysis revealed V2R and glycosylated AQP2 in biotinylated apical membranes of cilia (−) but not in cilia (+) cells. In addition, apical V2R was functional upon apical desmopressin (DDAVP) treatment by demonstrating increased cAMP...

Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals

Narita, Keishi; Kozuka-Hata, Hiroko; Nonami, Yuta; Ao-Kondo, Hiroko; Suzuki, Toshimitsu; Nakamura, Hideki; Yamakawa, Kazuhiro; Oyama, Masaaki; Inoue, Takafumi; Takeda, Sen
Fonte: The Company of Biologists Publicador: The Company of Biologists
Tipo: Artigo de Revista Científica
Publicado em 29/06/2012 EN
Relevância na Pesquisa
27.3%
Cilia are structurally and functionally diverse organelles, whose malfunction leads to ciliopathies. While recent studies have uncovered common ciliary transport mechanisms, limited information is available on the proteome of cilia, particularly that of sensory subtypes, which could provide insight into their functional and developmental diversities. In the present study, we performed proteomic analysis of unique, multiple 9+0 cilia in choroid plexus epithelial cells (CPECs). The analysis of juvenile swine CPEC cilia identified 868 proteins. Among them, 396 were shared with the proteome of 9+0 photoreceptor cilia (outer segment), whereas only 152 were shared with the proteome of 9+2 cilia and flagella. Various signaling molecules were enriched in a CPEC-specific ciliome subset, implicating multiplicity of sensory functions. The ciliome also included molecules for ciliary motility such as Rsph9. In CPECs from juvenile swine or adult mouse, Rsph9 was localized to a subpopulation of cilia, whereas they were non-motile. Live imaging of mouse choroid plexus revealed that neonatal CPEC cilia could beat vigorously, and the motility waned and was lost within 1–2 weeks. The beating characteristics of neonatal CPEC cilia were variable and different from those of typical 9+2 cilia of ependyma...

Primary Cilia Are Lost in Preinvasive and Invasive Prostate Cancer

Hassounah, Nadia B.; Nagle, Ray; Saboda, Kathylynn; Roe, Denise J.; Dalkin, Bruce L.; McDermott, Kimberly M.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 02/07/2013 EN
Relevância na Pesquisa
27.3%
Prostate cancer is the second most commonly diagnosed cancer in men worldwide. Little is known about the role of primary cilia in preinvasive and invasive prostate cancer. However, reduced cilia expression has been observed in human cancers including pancreatic cancer, renal cell carcinoma, breast cancer, cholangiocarcinoma, and melanoma. The aim of this study was to characterize primary cilia expression in preinvasive and invasive human prostate cancer, and to investigate the correlation between primary cilia and the Wnt signaling pathway. Human prostate tissues representative of stages of prostate cancer formation (normal prostate, prostatic intraepithelial neoplasia (PIN), and invasive prostate cancer (including perineural invasion)) were stained for ciliary proteins. The frequency of primary cilia was determined. A decrease in the percentage of ciliated cells in PIN, invasive cancer and perineural invasion lesions was observed when compared to normal. Cilia lengths were also measured to indirectly test functionality. Cilia were shorter in PIN, cancer, and perineural invasion lesions, suggesting dysfunction. Primary cilia have been shown to suppress the Wnt pathway. Increased Wnt signaling has been implicated in prostate cancer. Therefore...

Primary cilia and kidney injury: current research status and future perspectives

Wang, Shixuan; Dong, Zheng
Fonte: American Physiological Society Publicador: American Physiological Society
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.32%
Cilia, membrane-enclosed organelles protruding from the apical side of cells, can be divided into two classes: motile and primary cilia. During the past decades, motile cilia have been intensively studied. However, it was not until the 1990s that people began to realize the importance of primary cilia as cellular-specific sensors, particularly in kidney tubular epithelial cells. Furthermore, accumulating evidence indicates that primary cilia may be involved in the regulation of cell proliferation, differentiation, apoptosis, and planar cell polarity. Many signaling pathways, such as Wnt, Notch, Hedgehog, and mammalian target of rapamycin, have been located to the primary cilia. Thus primary cilia have been regarded as a hub that integrates signals from the extracellular environment. More importantly, dysfunction of this organelle may contribute to the pathogenesis of a large spectrum of human genetic diseases, named ciliopathies. The significance of primary cilia in acquired human diseases such as hypertension and diabetes has gradually drawn attention. Interestingly, recent reports disclosed that cilia length varies during kidney injury, and shortening of cilia enhances the sensitivity of epithelial cells to injury cues. This review briefly summarizes the current status of cilia research and explores the potential mechanisms of cilia-length changes during kidney injury as well as provides some thoughts to allure more insightful ideas and promotes the further study of primary cilia in the context of kidney injury.

Intraflagellar Transport Gene Expression Associated with Short Cilia in Smoking and COPD

Hessel, Justina; Heldrich, Jonna; Fuller, Jennifer; Staudt, Michelle R.; Radisch, Sharon; Hollmann, Charleen; Harvey, Ben-Gary; Kaner, Robert J.; Salit, Jacqueline; Yee-Levin, Jenny; Sridhar, Sriram; Pillai, Sreekumar; Hilton, Holly; Wolff, Gerhard; Bitte
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 20/01/2014 EN
Relevância na Pesquisa
27.3%
Smoking and COPD are associated with decreased mucociliary clearance, and healthy smokers have shorter cilia in the large airway than nonsmokers. We hypothesized that changes in cilia length are consistent throughout the airway, and we further hypothesized that smokers with COPD have shorter cilia than healthy smokers. Because intraflagellar transport (IFT) is the process by which cilia of normal length are produced and maintained, and alterations in IFT lead to short cilia in model organisms, we also hypothesized that smoking induces changes in the expression of IFT-related genes in the airway epithelium of smokers and smokers with COPD. To assess these hypotheses, airway epithelium was obtained via bronchoscopic brushing. Cilia length was assessed by measuring 100 cilia (10 cilia on each of 10 cells) per subject and Affymetrix microarrays were used to evaluate IFT gene expression in nonsmokers and healthy smokers in 2 independent data sets from large and small airway as well as in COPD smokers in a data set from the small airway. In the large and small airway epithelium, cilia were significantly shorter in healthy smokers than nonsmokers, and significantly shorter in COPD smokers than in both healthy smokers and nonsmokers. The gene expression data confirmed that a set of 8 IFT genes were down-regulated in smokers in both data sets; however...

Cilia María Porras, Alba Mery y Gladys Becerra, en el parque central frente all templo de San Juan Bautista; 502390; 502390

Fonte: Biblioteca Departamental Jorge Garces Borrero Publicador: Biblioteca Departamental Jorge Garces Borrero
Tipo: historicalDocument; other; Documento histórico Formato: JPEG; 6.5X10
SPA
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47.1%
Cilia María Porras, Alba Mery y Gladys Becerra, en el parque central frente all templo de San Juan Bautista. Guacarí. C. 1960.; El Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca es responsabilidad de la Biblioteca Departamental del Valle Jorge Garcés Borrero, por convenio de cooperación suscrito con la Secretaria del Cultura Departamental, con el fin de aunar esfuerzos para su conservación, preservación y divulgación del Archivo entre la comunidad Vallecaucana, especialmente entre los estudiantes e investigadores que visitan la Biblioteca, propiciando el su uso y consulta permanente. La universidad Icesi es un colaborador en el proceso de difusión, facilitando la tecnología que permite la consulta de las imágenes.; Valle del Cauca, Gobernación; Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca - Biblioteca Departamental Jorge Garces Borrero; Cilia María Porras, Alba Mery y Gladys Becerra, en el parque central frente all templo de San Juan Bautista. Guacarí. C. 1960.; Personajes: Cilia María Porras, Alba Mery y Gladys Becerra.; Tipo de fotografía: COLOR; Lugar de la toma: Por identificar

Cilia María Porras y su hijo Jorge Juan Porras, sentados en el parque principa a la sombra del centenario samán; 502392; 502392

FOTO LINE
Fonte: Biblioteca Departamental Jorge Garces Borrero Publicador: Biblioteca Departamental Jorge Garces Borrero
Tipo: historicalDocument; other; Documento histórico Formato: JPEG; 6.5X8
SPA
Relevância na Pesquisa
47.1%
Cilia María Porras y su hijo Jorge Juan Porras, sentados en el parque principa a la sombra del centenario samán. Guacarí. C. 1960.; El Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca es responsabilidad de la Biblioteca Departamental del Valle Jorge Garcés Borrero, por convenio de cooperación suscrito con la Secretaria del Cultura Departamental, con el fin de aunar esfuerzos para su conservación, preservación y divulgación del Archivo entre la comunidad Vallecaucana, especialmente entre los estudiantes e investigadores que visitan la Biblioteca, propiciando el su uso y consulta permanente. La universidad Icesi es un colaborador en el proceso de difusión, facilitando la tecnología que permite la consulta de las imágenes.; Valle del Cauca, Gobernación; Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca - Biblioteca Departamental Jorge Garces Borrero; Cilia María Porras y su hijo Jorge Juan Porras, sentados en el parque principa a la sombra del centenario samán. Guacarí. C. 1960.; Personajes: Jorge Juan Porras y Cilia María Porras.; Tipo de fotografía: COLOR; Lugar de la toma: PARQUE PPAL

Detállese la amplitud de este patio interior de esta casa 1977; 103590; 103590

Fonte: Biblioteca Departamental Jorge Garces Borrero Publicador: Biblioteca Departamental Jorge Garces Borrero
Tipo: historicalDocument; other; Documento histórico Formato: JPEG; Por definir
SPA
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36.8%
Detállese la amplitud de este patio interior de esta casa 1977; El Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca es responsabilidad de la Biblioteca Departamental del Valle Jorge Garcés Borrero, por convenio de cooperación suscrito con la Secretaria del Cultura Departamental, con el fin de aunar esfuerzos para su conservación, preservación y divulgación del Archivo entre la comunidad Vallecaucana, especialmente entre los estudiantes e investigadores que visitan la Biblioteca, propiciando el su uso y consulta permanente. La universidad Icesi es un colaborador en el proceso de difusión, facilitando la tecnología que permite la consulta de las imágenes.; Valle del Cauca, Gobernación; Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca - Biblioteca Departamental Jorge Garces Borrero; Detállese la amplitud de este patio interior de esta casa 1977; Personajes: Cilia Arana; Tipo de fotografía: COLOR; Lugar de la toma: Casa de la familia Arana, Bugalagrande.

Detállese la amplitud de este patio interior de esta casa d1977; 103590; 103590

Fonte: Biblioteca Departamental Jorge Garces Borrero Publicador: Biblioteca Departamental Jorge Garces Borrero
Tipo: historicalDocument; other; Documento histórico Formato: JPEG; Por definir
SPA
Relevância na Pesquisa
36.8%
Detállese la amplitud de este patio interior de esta casa d1977; El Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca es responsabilidad de la Biblioteca Departamental del Valle Jorge Garcés Borrero, por convenio de cooperación suscrito con la Secretaria del Cultura Departamental, con el fin de aunar esfuerzos para su conservación, preservación y divulgación del Archivo entre la comunidad Vallecaucana, especialmente entre los estudiantes e investigadores que visitan la Biblioteca, propiciando el su uso y consulta permanente. La universidad Icesi es un colaborador en el proceso de difusión, facilitando la tecnología que permite la consulta de las imágenes.; Valle del Cauca, Gobernación; Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca - Biblioteca Departamental Jorge Garces Borrero; Detállese la amplitud de este patio interior de esta casa d1977; Personajes: Cilia Arana; Tipo de fotografía: COLOR; Lugar de la toma: Casa de la familia Arana, Bugalagrande.

Detállese la amplitud de este patio interior de esta casa d1977; 103590; 103590

Fonte: Biblioteca Departamental Jorge Garces Borrero Publicador: Biblioteca Departamental Jorge Garces Borrero
Tipo: historicalDocument; other; Documento histórico Formato: JPEG; Por definir
SPA
Relevância na Pesquisa
36.8%
Detállese la amplitud de este patio interior de esta casa d1977; El Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca es responsabilidad de la Biblioteca Departamental del Valle Jorge Garcés Borrero, por convenio de cooperación suscrito con la Secretaria del Cultura Departamental, con el fin de aunar esfuerzos para su conservación, preservación y divulgación del Archivo entre la comunidad Vallecaucana, especialmente entre los estudiantes e investigadores que visitan la Biblioteca, propiciando el su uso y consulta permanente. La universidad Icesi es un colaborador en el proceso de difusión, facilitando la tecnología que permite la consulta de las imágenes.; Valle del Cauca, Gobernación; Archivo del Patrimonio Fotográfico y Fílmico del Valle del Cauca - Biblioteca Departamental Jorge Garces Borrero; Detállese la amplitud de este patio interior de esta casa d1977; Personajes: Cilia Arana; Tipo de fotografía: COLOR; Lugar de la toma: Casa de la familia Arana, Bugalagrande.

Sensational Propellers: Novel Protein Functions in Cilia Assembly and Motility

Austin, Christina Anne
Fonte: Harvard University Publicador: Harvard University
Tipo: Thesis or Dissertation
EN_US
Relevância na Pesquisa
37.35%
Cilia and flagella are hair-like projections found on the surface of virtually every vertebrate cell. These microtubule-based organelles are historically known for their striking motility, a valuable tool for the manipulation of fluid environments. In addition, immotile (or 'primary') cilia play critical roles in cell signaling. More than ten human diseases have been linked to cilia function, with pleiotropic phenotypes including obesity, kidney and liver disease, skeletal abnormalities, situs defects, mental retardation, and sterility. In this dissertation, I first examine the function of Cep290, a putative master regulator of cilia biology, which is mutated in five human ciliopathies. I found that the zebrafish Cep290 protein was localized in a cell-type specific fashion to two distinct ciliary compartments: transition zones and centriolar satellites. Through morpholino knockdown, I demonstrated that Cep290 regulates the length of photoreceptor, Kupffer’s vesicle, and spinal canal cilia, while it was dispensable for normal cilia length in other tissues. Rescue of Cep290 associated cilia length defects by overexpression of cilia membrane proteins implicated Cep290 in cilia vesicle trafficking. Unexpectedly, I found that Cep290 deficiency in Kupffer’s vesicle and spinal canal resulted in cilia paralysis...

Lack of Dopaminergic Inputs Elongates the Primary Cilia of Striatal Neurons

Miyoshi, Ko; Kasahara, Kyosuke; Murakami, Shinki; Takeshima, Mika; Kumamoto, Natsuko; Sato, Asako; Miyazaki, Ikuko; Matsuzaki, Shinsuke; Sasaoka, Toshikuni; Katayama, Taiichi; Asanuma, Masato
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 15/05/2014 EN
Relevância na Pesquisa
27.3%
In the rodent brain, certain G protein-coupled receptors and adenylyl cyclase type 3 are known to localize to the neuronal primary cilium, a primitive sensory organelle protruding singly from almost all neurons. A recent chemical screening study demonstrated that many compounds targeting dopamine receptors regulate the assembly of Chlamydomonas reinhardtii flagella, structures which are analogous to vertebrate cilia. Here we investigated the effects of dopaminergic inputs loss on the architecture of neuronal primary cilia in the rodent striatum, a brain region that receives major dopaminergic projections from the midbrain. We first analyzed the lengths of neuronal cilia in the dorsolateral striatum of hemi-parkinsonian rats with unilateral lesions of the nigrostriatal dopamine pathway. In these rats, the striatal neuronal cilia were significantly longer on the lesioned side than on the non-lesioned side. In mice, the repeated injection of reserpine, a dopamine-depleting agent, elongated neuronal cilia in the striatum. The combined administration of agonists for dopamine receptor type 2 (D2) with reserpine attenuated the elongation of striatal neuronal cilia. Repeated treatment with an antagonist of D2, but not of dopamine receptor type 1 (D1)...