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Ação antibacteriana da cetramida associada às solucões irrigadoras sobre Enterococcus Faecalis

Nascimento, Camila Almeida
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Dissertação de Mestrado Formato: 83 f. : il. color
POR
Relevância na Pesquisa
17.51%
Pós-graduação em Odontologia - FOAR; The aim of this study was to evaluate the action of conventional endodontic solutions such as sodium hypochlorite and chlorhexidine associated with cetrimide and QMiX, a commercial product composed by chlorhexidine, EDTA and cetrimide, on biofilm and planktonic phase of Enterococcus faecalis (ATCC 29212). The evaluated solutions were: G1: 2.5% Sodium Hypochlorite (NaOCl); G2: 2.5% NaOCl + 0.2% cetrimide (CTR); G3: 2% Chlorhexidine (CLX) 2%; G4: 2% CLX + 0.2% CTR; G5: 0.2% CTR; G6: QMiX. Saline solution was used as negative control. The study was divided into two chapters. In Chapter 1, the antibacterial activity of the solutions on microorganisms in planktonic phase and biofilm was evaluated. For direct contact with E. faecalis in planktonic phase, 50 μL of bacterial suspension were placed in tubes containing 1.45 mL of each irrigation solution or associations and after 1 and 3 minutes, neutralizing solutions were used to stop of the antimicrobial action of endodontic solutions. Decimal serial dilutions were performed and the suspensions were plated in Tryptic Soy agar medium (TSa). Dentin bovine blocks were used as substrate for induction of biofilms for 14 days. The biofilms were placed in contact with the solutions evaluated and transferred to test tubes containing neutralizing solution. After agitation for 60 seconds to suspend the remaining cells...

Antimicrobial activity of root canal irrigants associated with cetrimide against biofilm and planktonic Enterococcus Faecalis

Nascimento, Camila Almeida; Tanomaru-Filho, Mario; Faria-Júnior, Norberto Batista de; Faria, Gisele; Guerreiro-Tanomaru, Juliane Maria
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 79-83
ENG
Relevância na Pesquisa
27.2%
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Aim: To evaluate the antibacterial activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) alone or associated with cetrimide (CTR), and QMiX against biofilm and planktonic Enterococcus faecalis (E. faecalis) [American type culture collection (ATCC) 29212]. Materials and methods: The solutions 2.5% NaOCl, 2.5% NaOCl + 0.2% CTR, 2% CHX, 2% CHX + 0.2% CTR, 0.2% CTR, and QMiX were evaluated. E. faecalis biofilms were induced for 14 days on bovine dentin blocks. The irrigants were evaluated after contact with E. faecalis suspension and biofilm for 1 and 3 minutes. After that, serial decimal dilutions were made and plated on tryptic soy agar (TSA) medium. Plates were incubated for 24 hours at 37°C and the colony-forming unit (CFU) 1 ml was determined. Data were subjected to ANOVA and Tukey’s tests at 5% significance. Results: All microorganisms were eliminated by direct contact of the irrigants with planktonic cells. Only NaOCl and NaOCl + CTR were able to completely eliminate the microorganisms by direct contact with E. faecalis biofilm. CHX presented effectiveness similar to CHX + CTR CTR, and QMiX after 1 minute of contact and similar to NaOCl and NaOCl + CTR after 3 minutes (p > 0.05)...

Modified Pseudomonas agar: new differential medium for the detection/enumeration of Pseudomonas aeruginosa in mineral water

Ramalho, Rita; Cunha, Joaquim; Teixeira, Paula; Gibbs, Paul A.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em //2002 ENG
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27.51%
Pseudomonas aeruginosa has been implicated as a foodborne and waterborne pathogen and is now considered a primary infectious agent. In the present study, the survival of P. aeruginosa inoculated in mineral water was evaluated by drop counts on Pseudomonas Agar Base (PAB), PAB with CN supplement X107, PAB with cetrimide, PAB with nalidixic acid, and these media with added FeSO4. Initial counts, before starvation, were the same in all media tested. Following this period, P. aeruginosa became sensitive to PAB with added cetrimide. The addition of FeSO4 did not improve the recovery of stressed P. aeruginosa but gave colonies a typical dark brown colour being easily differentiated from other species that can grow at 42 C. The modified Pseudomonas agar medium was also tested with several P. aeruginosa strains, other species of Pseudomonas, and other genera. Only P. aeruginosa strains (pyocyanin positive) produced the typical colonies. Our results demonstrate that Pseudomonas agar with ferrous sulphate, used for the differentiation of P. aeruginosa colonies, and nalidixic acid, used as an inhibitor of Gram-positive bacteria, might be a useful medium for the detection of injured P. aeruginosa in mineral water.

Pulp Tissue Dissolution Capacity of Sodium Hypochlorite Combined with Cetrimide and Polypropylene Glycol

Almeida,Luiza Helena Silva de; Leonardo,Natalia Gomes e Silva; Gomes,Ana Paula Neutzling; Giardino,Luciano; Souza,Erick Miranda; Pappen,Fernanda Geraldo
Fonte: Fundação Odontológica de Ribeirão Preto Publicador: Fundação Odontológica de Ribeirão Preto
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2013 EN
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37.84%
This study evaluated the influence of the addition of cetrimide and polypropylene glycol to sodium hypochlorite (NaOCl) on its capacity to dissolve pulp tissue. Bovine pulp fragments with standardized weight and volume were immersed for 5, 15 and 30 min in 2 mL of NaOCl and Hypoclean (NaOCl added with cetrimide and polypropylene glycol) solutions at 5.25%, 2.5%, 1%, 0.5% and 0.25% and afterwards re-weighted. Distilled water was used as a control. The percentage of tissue loss was considered for statistical analysis (univariate ANOVA, SPSS, v. 17.0) at 5% significance level. There was no tissue dissolution in the control group. NaOCl added with surfactants (Hypoclean) dissolved more pulp tissue (p<0.05) than NaOCl alone. Tissue dissolution was directly dependent on the concentration of solutions (p<0.05), and also on the time range (p<0.05). The combination of NaOCl at high and low concentrations with the surfactants cetrimide and polypropylene glycol increased significantly its capacity to dissolve pulp tissue.

Effect of a Surfactant on the Antimicrobial Activity of Sodium Hypochlorite Solutions

Bolfoni,Marcos Rodolfo; Ferla,Marcelo dos Santos; Sposito,Otávio da Silva; Giardino,Luciano; Jacinto,Rogério de Castilho; Pappen,Fernanda Geraldes
Fonte: Fundação Odontológica de Ribeirão Preto Publicador: Fundação Odontológica de Ribeirão Preto
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2014 EN
Relevância na Pesquisa
27.2%
The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean(r), a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.

Phenotypic and genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in siedlce (Poland)

Wolska,Katarzyna; Kot,Barbara; Jakubczak,Antoni
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2012 EN
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A total of 62 Pseudomonas aeruginosa strains isolated from two hospitals in Siedlce (Poland) were studied by repetitive element based PCR (rep-PCR) using BOX primer. BOX-PCR results revealed the presence of 7 numerous genotypes and 31 unique patterns among isolates. Generally, the strains of P. aeruginosa were characterized by resistance to many antibiotics tested and by differences in serogroups and types of growth on cetrimide agar medium. However, the P. aeruginosa strains isolated from faeces showed much lower phenotypic and genotypic variations in comparison with strains obtained from other clinical specimens. It was observed that genetic techniques supported by phenotypic tests have enabled to conduct a detailed characterization of P. aeruginosa strains isolated from a particular environment at a particular time.

Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine

NAVARRO-ESCOBAR,Esther; BACA,Pilar; GONZALEZ-RODRIGUEZ,Maria Paloma; ARIAS-MOLIZ,Maria Teresa; RUIZ,Matilde; FERRER-LUQUE,Carmen Maria
Fonte: Faculdade De Odontologia De Bauru - USP Publicador: Faculdade De Odontologia De Bauru - USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2013 EN
Relevância na Pesquisa
27.2%
Objective To assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of Enterococcus faecalis in filled root canals. Material and Methods Seventy single-root canals from extracted teeth were prepared using ProTaper instruments. The irrigation protocol accomplished an alternating irrigation with 5 mL of 2.5% sodium hypochlorite (NaOCI) and 17% EDTA between each file. The teeth were randomly divided into four experimental groups (n=15) according to the final irrigation regimen: group 1, without final irrigation; group 2, irrigation with 10 mL 2.0% chlorhexidine (CHX); group 3, with a final application of EC40™; and group 4, irrigation with the combination (1:1) of 0.2% CHX + 0.1% cetrimide (CTR). All the teeth were mounted in a two-chamber apparatus and the coronal access was exposed to E. faecalis. The presence of turbidity in the BHI broth over a period of 180 days was observed. The Friedman test was used for statistical analysis. Results EC40™ varnish showed the least leakage at 180 days, and was statistically similar to 2% CHX. No significant differences were observed between the group without final irrigation and the 2% CHX group or 0.2% CHX + 0.1% CTR. Conclusions In this ex vivo study...

Decalcifying efficacy of different irrigating solutions: effect of cetrimide addition

Claudio,POGGIO; Alberto,DAGNA; Marco,COLOMBO; Andrea,SCRIBANTE; Marco,CHIESA
Fonte: Sociedade Brasileira de Pesquisa Odontológica - SBPqO Publicador: Sociedade Brasileira de Pesquisa Odontológica - SBPqO
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2014 EN
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27.71%
The objective of the present study was to evaluate and compare the influence of cetrimide on decalcifying capability of different irrigating solutions. Fifteen maxillary central incisor teeth has been collected. The canals were prepared in order to obtain four samples from each root. The specimens were randomly divided into 6 experimental groups (n=10) according to tested irrigating agents. Irrigating agents consisted in different composition of EDTA and citric acid solutions, addicted or not with cetrimide. Each specimen was submitted to three successive 5-min immersions in each solution. After exposures, the concentration of Ca2+ extracted was measured by inductively coupled plasma-atomic emission spectrometry (ICP-AES). Data were analysed by means of Kruskal Wallis and Mann Whitney tests. Significance was predetermined at p < 0.05. For all irrigants, the amounts of Ca2+ extracted from root canal dentin samples at 10 minutes were not significantly different from values reported after 15 minutes respectively. Therefore, for all irrigants tested, 10 minutes of application are sufficient to obtain maximum Ca2+ release. Moreover citric acid based agents observed a higher release of Ca2+. The addition of cetrimide did not affect the decalcifying capability of the EDTA and citric acid solutions.

Cutaneous necrosis due to cetrimide application.

August, P J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 11/01/1975 EN
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Reports of necrosis caused by quaternary ammonium compounds, such as cetrimide, are rare. The case is reported of a 77-year-old woman who was admitted to hospital for four months with cutaneous necrosis of the left foot and leg owing to the topical application of cetrimide powder.

Cetrimide burn in an infant

Mercer, D. M.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /07/1983 EN
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27.2%
A case of chemical burns due to cetrimide applied to the nappy area of an infant is recorded.

Eradication of enterococci biofilms by lactic acid alone and combined with chlorhexidine and cetrimide

Arias-Moliz, María T.; Baca, Pilar; Ordóñez-Becerra, Santiago; González-Rodríguez, María P.; Ferrer-Luque, Carmen M.
Fonte: Medicina Oral S.L. Publicador: Medicina Oral S.L.
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.2%
Objective: The antimicrobial activity of lactic acid (LA) alone or in combination with chlorhexidine (CHX) and cetrimide (CTR) against three Enterococcus faecalis strains, E. faecalis ATCC 29212, E. faecalis EF-D1 and E. faecalis U-1765, one Enterococcus durans strain and one dual-species biofilm was investigated. Study Design: The irrigating solutions tested were 20%, 15%, 10%, 5% and 2.5% LA, alone and in combination with 2% CHX and with 0.2% CTR. The biofilms were grown in the MBECTM high-throughput device for 24 hours and exposed to the solutions for 30 seconds and 1 minute. “Eradication” was defined as 100% bacterial kill. Results: Twenty percent LA eradicated all enterococci biofilms after 30 seconds contact time. The association of LA + 0.2% CTR achieved better results than LA alone, in contrast with the results obtained using LA + 2% CHX. E. durans was eradicated by all the tested solutions at 1 minute. The dual-species biofilm, E. faecalis ATCC 29212 + E. durans, gave intermediate values of the pure cultures. Conclusions: LA is capable of eradicating enterococci biofilm at a concentration of 20%. The combination of lower concentrations with 0.2% CTR achieved eradication after 1 minute.

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide against Enterococcus faecalis biofilm

Martos, Josué; Ferrer Luque, Carmen Maria; González-Rodríguez, Maria Paloma; Arias-Moliz, Maria Teresa; Baca, Pilar
Fonte: Akadémiai Kiadó Publicador: Akadémiai Kiadó
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.2%
The Enterococcus faecalis bacteria have been identified as the most commonly recovered species from teeth with persistent endodontic infections. The antimicrobial activity of essential oils and chloroform (CHL), alone and in association with various concentrations of cetrimide (CTR), against biofilm of Enterococcus faecalis was investigated. Solutions of CHL, eucalyptus oil (EO) and orange oil (OO) associated with CTR at 0.3%, 0.2%, 0.1%, and 0.05% were used to determine antimicrobial activity by exposing treated bovine dentine blocks to E. faecalis. Biofilms grown in the dentine blocks for 7 days were exposed to solutions for 2 and 5 min. Biofilm reduction between OO and EO at 2 min did not show any significant differences; however, OO had a higher kill percentage of biofilms than did the eucalyptus oil at 5 min (p < 0.01). Combinations with CTR at all concentrations achieved a 100% kill rate at 2 and 5 min. The association of CTR with solvent agents achieved the maximum antimicrobial activity against E. faecalis biofilms in dentine.

Residual activity of cetrimide and chlorhexidine on Enterococcus faecalis-infected root canals

María Ferrer-Luque, Carmen; Teresa Arias-Moliz, María; Ruíz-Linares, Matilde; Elena Martínez García, María; Baca, Pilar
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.84%
Effective final irrigation regimen is an important step in order to achieve better disinfection and ensure residual antimicrobial effects after root canal preparation. The aim of this study was to compare the residual antimicrobial activity of 0.2% cetrimide, and 0.2% and 2% chlorhexidine in root canals infected with Enterococcus faecalis. Biofilms of E. faecalis were grown on uniradicular roots for 4 weeks. After root canal preparation, root canals were irrigated with 17% ethylenediaminetetraacetic acid (EDTA) to remove the smear layer. The roots were randomly divided into three experimental groups (n=26) according to the final irrigating solution: Group I, 5 mL 0.2% cetrimide; Group II, 5 mL 0.2% chlorhexidine; and Group III, 5 mL 2% chlorhexidine. Samples were collected for 50 days to denote the presence of bacterial growth. The proportion of ungrown specimens over 50 days was evaluated using the nonparametric Kaplan–Meier survival analysis. Differences among groups were tested using the log-rank test and the level of statistical significance was set at P<0.05. The highest survival value was found with 2% chlorhexidine, showing statistically significant differences from the other two groups. At 50 days, E. faecalis growth was detected in 69.23% specimens in Groups I and II...

Antibacterial and residual antimicrobial activities against Enterococcus faecalis biofilm: A comparison between EDTA, chlorhexidine, cetrimide, MTAD and QMix

Zhang, Rui; Chen, Min; Lu, Yan; Guo, Xiangjun; Qiao, Feng; Wu, Ligeng
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em 06/08/2015 EN
Relevância na Pesquisa
27.2%
We compared the antibacterial and residual antimicrobial activities of five root canal irrigants (17% EDTA,2% chlorhexidine,0.2% cetrimide, MTAD, and QMix) in a model of Enterococcus faecalis biofilm formation. Sixty dentin blocks with 3-week E. faecalis biofilm were divided into six equal groups and flushed with irrigant for 2 min or left untreated. A blank control group was also established. Antibacterial activities of the irrigants were evaluated by counting colony forming units. To test residual antimicrobial activities, 280 dentin blocks were divided into seven equal groups and flushed with irrigant for 2 min or left untreated and then incubated with E. faecalis suspension for 48 h, or used as a blank. No bacteria were observed in the blank control group. The number of viable E. faecalis was significantly fewer in the irrigant-treated groups compared with the untreated control (P < 0.05). Among the five irrigants, QMix had the strongest antibacterial activity. Residual antimicrobial activities of CHX were significantly higher at 12 h, 24 h and 36 h compared to untreated control (P < 0.05). All five root canal irrigants were effective to some extent against E. faecalis, but QMix and CHX had the strongest, and CHX the longest (up to 36 h)...

Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine

Navarro Escobar, Esther; Baca Garc??a, Pilar; Gonz??lez Rodr??guez, Mar??a Paloma; Arias-Moliz, Teresa; Ruiz-Linares, Matilde; Ferrer Luque, Carmen Mar??a
Fonte: Universidade de Sao Paulo. Faculdade de Odontologia de Bauru Publicador: Universidade de Sao Paulo. Faculdade de Odontologia de Bauru
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
27.2%
Objective To assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of Enterococcus faecalis in filled root canals. Material and Methods Seventy single-root canals from extracted teeth were prepared using ProTaper instruments. The irrigation protocol accomplished an alternating irrigation with 5 mL of 2.5% sodium hypochlorite (NaOCI) and 17% EDTA between each file. The teeth were randomly divided into four experimental groups (n=15) according to the final irrigation regimen: group 1, without final irrigation; group 2, irrigation with 10 mL 2.0% chlorhexidine (CHX); group 3, with a final application of EC40???; and group 4, irrigation with the combination (1:1) of 0.2% CHX + 0.1% cetrimide (CTR). All the teeth were mounted in a two-chamber apparatus and the coronal access was exposed to E. faecalis. The presence of turbidity in the BHI broth over a period of 180 days was observed. The Friedman test was used for statistical analysis. Results EC40??? varnish showed the least leakage at 180 days, and was statistically similar to 2% CHX. No significant differences were observed between the group without final irrigation and the 2% CHX group or 0.2% CHX + 0.1% CTR. Conclusions In this ex vivo study...

Antimicrobial activity of alexidine, chlorhexidine and cetrimide against Streptococcus mutans biofilm

Ruiz-Linares, Matilde; Ferrer Luque, Carmen Mar??a; Arias-Moliz, Teresa; Castro, Paula de; Aguado, Beatriz; Baca Garc??a, Pilar
Fonte: Biomed Central Publicador: Biomed Central
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
37.71%
Background The use of antimicrobial solutions has been recommended to disinfect demineralized dentin prior to placing the filling material. The aim of this study was to evaluate the ability of several antimicrobials in controlling Streptococcus mutans (SM) biofilm formed in dentin. Methods Antimicrobial activity of 0.2% and 2% chlorhexidine (CHX), 0.2% cetrimide (CTR) and 0.2%, 0.5%, 1% and 2% alexidine (ALX) was assayed on 1-week SM biofilm formed on standardized coronal dentin blocks. Results of SM biofilm antimicrobial activity by different protocols were expressed as the kill percentage of biofilm and the term ???eradication??? was used to denote the kill of 100% of the bacterial population. To compare the efficacies of the different protocols the Student t test was used, previously subjecting data to the Anscombe transformation. Results All ALX concentrations tested and 0.2% CTR achieved a kill percentage higher than 99%, followed by 2% CHX with percentages above 96% (no statistically significant difference among them). Whereas 2% ALX and 0.2% CTR respectively showed eradication in 10 and 9 of the twelve specimens, 0.2% CHX did not produce eradication in any case. Conclusions The present study shows that, when used for one minute...

Eficácia do hipoclorito de sódio com surfactante na desinfecção de cones de guta-percha e análise de alterações superficiais em MEV

Vitali, Filipe Colombo
Fonte: Universidade Federal de Santa Catarina Publicador: Universidade Federal de Santa Catarina
Tipo: Trabalho de Conclusão de Curso Formato: 39 f.
PT_BR
Relevância na Pesquisa
27.84%
TCC (graduação) - Universidade Federal de Santa Catarina. Centro de Ciências da Saúde. Odontologia.; A desinfecção do sistema de canais radiculares é um dos objetivos a ser alcançado na terapia endodôntica e, para tal, é importante que todas as etapas do tratamento sejam realizadas sob condições assépticas. Os cones de guta-percha utilizados para a obturação dos canais radiculares têm propriedade termoplástica, não podendo passar pelo processo convencional de esterilização. O hipoclorito de sódio (NaOCl) tem sido utilizado para a desinfecção química dos mesmos, porém a eficácia dos compostos com a adição de surfactante ainda não foi totalmente elucidada. O objetivo deste estudo foi avaliar, in vitro, a eficácia do NaOCl a 1% e a 5,25%, sem e com surfactante, na desinfecção química de cones de guta-percha nos tempos de 30 s e 1 min. Adicionalmente, foram avaliadas em MEV as alterações superficiais dos cones de guta-percha após o uso das soluções de NaOCl com ou sem surfactante. Para o estudo, cones de guta-percha com calibre 80 (n = 120) foram desinfectados com NaOCl a 5,25% por 30 min. Posteriormente, foram contaminados (n = 108) com Enteroccocus faecalis e imersos por 30 s (A) ou 1 min (B) (n = 12) nas seguintes soluções: G1A e G1B: NaOCl a 1%; G2A e G2B: NaOCl a 1% com cetrimide 2%; G3A e G3B: NaOCl a 5...

Use of an improved cetrimide agar medium and other culture methods for Pseudomonas aeruginosa

Brown, V. I.; Lowbury, E. J. L.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1965 EN
Relevância na Pesquisa
27.51%
In a comparison of two selective media for Ps. aeruginosa containing 0·03% cetrimide, stronger fluorescence was obtained from growth on the medium prepared with King's medium B as the base (CTA 2); although Ps. aeruginosa was not isolated more frequently from burns on this medium than from cetrimide agar made with a Lemco base (CTA 1), results were easier to assess and there were fewer cases of doubtful fluorescence on CTA 2 than on CTA 1.

Disinfection of upper gastrointestinal fibreoptic endoscopy equipment: an evaluation of a cetrimide chlorhexidine solution and glutaraldehyde.

Carr-Locke, D L; Clayton, P
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /10/1978 EN
Relevância na Pesquisa
27.51%
There is little information available on the bacteriological contamination of upper gastrointestinal fibreoptic endoscopes during routine use and the effects of 'disinfecting solutions'. A bacteriological evaluation was therefore made of cleaning an endoscope and its ancillary equipment with (1) water, (2) an aqueous solution of 1% cetrimide with 0.1% chlorhexidine, and (3) activated aqueous 2% glutaraldehyde. All equipment, but particularly the endoscope itself, was found to be heavily contaminated after use with a wide variety of organisms of which 53% were Gram positive. Cleaning the endoscope and ancillary equipment with water and the cetrimide/chlorhexidine solution alone or in combination was inadequate to produce disinfection but immersion in glutaraldehyde for two minutes consistently produced sterile cultures with our sampling technique. A rapid and simple method for disinfection of endoscopic equipment is therefore recommended and we think this is especially suitable for busy endoscopy units.

Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine

NAVARRO-ESCOBAR, Esther; BACA, Pilar; GONZÁLEZ-RODRÍGUEZ, María Paloma; ARIAS-MOLIZ, María Teresa; RUIZ, Matilde; FERRER-LUQUE, Carmen María
Fonte: Universidade de São Paulo. Faculdade de Odontologia de Bauru Publicador: Universidade de São Paulo. Faculdade de Odontologia de Bauru
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/01/2013 ENG
Relevância na Pesquisa
27.2%
Objective To assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of Enterococcus faecalis in filled root canals. Material and Methods Seventy single-root canals from extracted teeth were prepared using ProTaper instruments. The irrigation protocol accomplished an alternating irrigation with 5 mL of 2.5% sodium hypochlorite (NaOCI) and 17% EDTA between each file. The teeth were randomly divided into four experimental groups (n=15) according to the final irrigation regimen: group 1, without final irrigation; group 2, irrigation with 10 mL 2.0% chlorhexidine (CHX); group 3, with a final application of EC40™; and group 4, irrigation with the combination (1:1) of 0.2% CHX + 0.1% cetrimide (CTR). All the teeth were mounted in a two-chamber apparatus and the coronal access was exposed to E. faecalis. The presence of turbidity in the BHI broth over a period of 180 days was observed. The Friedman test was used for statistical analysis. Results EC40™ varnish showed the least leakage at 180 days, and was statistically similar to 2% CHX. No significant differences were observed between the group without final irrigation and the 2% CHX group or 0.2% CHX + 0.1% CTR. Conclusions In this ex vivo study...