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In situ effect of chewing gum containing CPP–ACP on the mineral precipitation of eroded bovine enamel

Prestes, Leticia de Almeida; Souza, Beatriz Martines de; Comar, Lívia Picchi; Salomão, Priscila Maria Aranda; Honório, Daniela Rios; Magalhães, Ana Carolina
Fonte: Elsevier; Guildford Publicador: Elsevier; Guildford
Tipo: Artigo de Revista Científica
ENG
Relevância na Pesquisa
27.33%
Objectives: Stimulation of salivary flow is considered a preventive strategy for dental erosion. Alternatively, products containing calcium phosphate, such as a complex of casein phosphopeptide–amorphous calcium phosphate (CPP–ACP), have also been tested against dental erosion. Therefore, this in situ study analyzed the effect of chewing gum containing CPP–ACP on the mineral precipitation of initial bovine enamel erosion lesions. Methods: Twelve healthy adult subjects wore palatal appliances with two eroded bovine enamel samples. The erosion lesions were produced by immersion in 0.1% citric acid (pH 2.5) for 7 min. During three experimental crossover in situ phases (1 day each), the subjects chewed a type of gum, 3 times for 30 min, in each phase: with CPP–ACP (trident total), without CPP–ACP (trident), and no chewing gum (control). The Knoop surface microhardness was measured at baseline, after erosion in vitro and the mineral precipitation in situ. The differences in the degree of mineral precipitation were analyzed using repeated measures (RM-) ANOVA and post hoc Tukey’s test ( p < 0.05). Results: Significant differences were found among the remineralizing treatments ( p < 0.0001). Chewing gum (19% of microhardness recovery) improved the mineral precipitation compared to control (10%) and the addition of CPP–ACP into the gum promoted the best mineral precipitation effect (30%). Conclusions: Under this protocol...

Estudo da progressão e inibição de lesões artificiais de cárie através de alterações da microdureza superficial; Progression and inhibition of artifical caries lesions in human and bovine substrate evaluated by surface microhardness

Rehder Neto, Francisco Carlos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 29/01/2009 PT
Relevância na Pesquisa
27.44%
Os objetivos do presente estudo foram avaliar a progressão e a inibição de lesões artificiais de cárie comparando-se substrato humano e bovino, e também avaliar potenciais agentes remineralizantes no controle da progressão destas lesões. Este trabalho foi divido em duas partes. Inicialmente, para validar o modelo de indução das lesões e comparar o substrato humano ao bovino, sessenta fragmentos de esmalte (30 humanos e 30 bovinos) e 60 fragmentos de dentina (30 humanos e 30 bovinos) medindo 4x4x2 foram embutidos, planificados e polidos e avaliados quanto à microdureza superficial (MDS, Knoop) utilizando-se carga estática e tempo de aplicação de 25g, por 5s para esmalte e 10 g por 10 segundos para dentina. Lesões incipientes de cárie foram induzidas através de um modelo de ciclos de pH. Os espécimes foram imersos por 1 hora em solução desmineralizante e por 22 horas em solução remineralizante, o que correspondia a um ciclo de pH. Novas medidas de MDS foram realizadas ao final de cada ciclo de pH, sendo que para dentina foram realizados 3 ciclos e para esmalte 8 ciclos. A Análise de Variância (ANOVA) foi conduzida seguindo-se o delineamento fatorial, 2x9, para esmalte; e 2x4 para dentina. Análises de Regressão foram utilizadas para determinar a equação do modelo que rege a perda de conteúdo mineral ao longo do tempo de indução das lesões de cárie. A Análise de Variância (ANOVA) demonstrou diferenças altamente significativas para o fator substrato (p<0...

Efeito in situ de uma goma de mascar contendo caseína fosfopeptídea - fosfato de cálcio amorfo (CPP-ACP) na erosão dentária; In situ effect of chewing gum containing casein phosphopeptide - amorphous calcium phosphate on dental erosion

Alencar, Catarina Ribeiro Barros de
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 29/05/2013 PT
Relevância na Pesquisa
27.37%
O presente trabalho avaliou o efeito in situ de uma goma de mascar comercialmente disponível contendo caseína fosfopeptídea - fosfato de cálcio amorfo (CPP-ACP) na erosão dentária. Para a primeira etapa do estudo (capacidade remineralizadora) utilizaram-se 72 blocos de esmalte humano selecionados pela dureza de superfície (SHi) e erodidos in vitro pela imersão em Coca Cola®, pH 2,4 por 3 min (avaliação da dureza - SHd). Os blocos foram randomizados entre os grupos: GI Trident Fresh® (sem CPP-ACP), GII controle (sem chiclete) e GIII Trident Total® (com CPPACP). Doze voluntários utilizaram dispositivos intrabucais palatinos por 24 h em 3 fases cruzadas. Nas fases de GI e GIII os voluntários mascaram um chiclete (30 min) e em todas as fases após 2h, a dureza foi avaliada (SHf1). Os blocos foram reposicionados e os dispositivos usados por mais 22 h (+ 3 ciclos de mastigação de chiclete - GI e GIII). A dureza foi reavaliada (SHf2) para cálculo do percentual de recuperação de dureza (%SHR) após 2 e 24h. Na segunda etapa do estudo (ciclagem erosiva) 48 blocos de esmalte humano hígidos foram aleatorizados entre os grupos (GI, GII e GIII) e 8 voluntários utilizaram dispositivos intrabucais palatinos em fases cruzadas de 7 dias cada (washout de 7 dias). O protocolo de ciclagem erosiva foi de 4 imersões diárias do dispositivo intrabucal em 150 ml de Coca Cola® durante 5 min. Nos grupos I e III após cada desafio erosivo e reinserção do dispositivo na cavidade bucal...

The effect of CPP-ACP and Nd:YAG laser on the bond strength of softened dentin

Kamozaki, Maria Beatriz Beber; Prakki, Anuradha; Perote, Letícia Carvalho Coutinho Costa; Gutierrez, Natalia Cortez; Pagani, Clovis
Fonte: Sociedade Brasileira de Pesquisa Odontológica - SBPqO Publicador: Sociedade Brasileira de Pesquisa Odontológica - SBPqO
Tipo: Artigo de Revista Científica Formato: 1-7
ENG
Relevância na Pesquisa
27.33%
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 2011/10827-4; The purpose of this study was to investigate the effect of CPP-ACP treatment and Nd:YAG laser on microtensile bond strength (µTBS) of softened dentin. Sixty samples were obtained from thirty sound third molars. All samples were submitted to dentin softening procedure, by the immersion of the specimens in 30 mL of Sprite Zero for 30min. Afterwards, the samples were randomly divided according to the CPP-ACP treatment: CG-Control group; MP-treated with CPP-ACP paste (MI Paste); MPP-treated with CPP-ACP+900 ppm NaF paste (MI Paste Plus). Each group was further divided according to bonding procedure: NL-No laser; L–Laser irradiation after adhesive application and before polymerization. The laser parameters used were 1.4 W, 10 Hz, 140 mJ/pulse, with an optic fiber of 320 µm, generating energy of 174 J/cm2 per pulse. All samples were restored with Clearfil SE Bond/Filtek Z350 XT. After 24 h, the restored samples were cut into beams (± 1 mm2 adhesive interface area) and subjected to a µTBS test. Data were analyzed by two-way ANOVA test and Holm-Sidak post-hoc method (α = 0.05). The treatment with CPP-ACP pastes did not significantly affect softened dentin µTBS (p = 0.070). Statistic revealed significant reduction on µTBS values for CG/L...

Efeito da aplicação de fosfopeptidio de caseina-fosfato de calcio amorfo (CPP-ACP) associada ao protocolo adesivo na resistencia de união de um selante de fossulas e fissuras; Effect of the casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) associated to adhesive procedures on bond strength of a pit and fissure sealant

Boniek Castillo Dutra Borges
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 26/03/2010 PT
Relevância na Pesquisa
27.29%
O objetivo deste trabalho foi investigar, in vitro, o efeito da aplicação de uma pasta contendo fosfopeptídeo de caseína - fosfato de cálcio amorfo (CPP-ACP) (MI Paste - MI) previamente ao protocolo adesivo na resistência da união de um selante resinoso de fóssulas e fissuras ao esmalte dental humano. Foram utilizados 98 espécimes de esmalte proximal de terceiros molares totalmente erupcionados, divididos em 14 grupos experimentais (n=7): G1/G8- condicionamento ácido (CA) (Scotchbond Etchant), sem a aplicação de sistema adesivo; G2/G9- CA + resina hidrófoba de um sistema adesivo convencional de três passos (Scotchbond Multipurpose Plus); G3/G10- CA + sistema adesivo convencional de dois passos (Single Bond 2); G4/G11 - CA + sistema adesivo autocondicionante de passo único (Clearfil S3 Bond); G5/G12- CA + sistema adesivo autocondicionante de dois passos (Clearfil SE Bond); G6/G13- sistema adesivo autocondicionante de dois passos; G7/G14- adesivo autocondicionante de passo único. MI foi aplicada previamente ao protocolo adesivo apenas nos grupos de G1 a G7. Após selamento das superfícies (FluroShield), foram confeccionados blocos retangulares (palitos) (~0,7mm2 de área seccional) para o ensaio de microtração. O teste foi efetuado imediatamente após confecção das amostras por meio da Máquina de Ensaio Universal...

Avaliação do efeito remineralizador da caseína fosfato de cálcio fosfopeptídeo amorfo - cpp-acp nas lesões iniciais de cárie em dentes decíduos e permanentes; estudo “in vivo”

Melo, Loyane Franio Batista de
Fonte: Universidade de Brasília Publicador: Universidade de Brasília
Tipo: Dissertação
POR
Relevância na Pesquisa
27.37%
Dissertação (mestrado)—Universidade de Brasília, Centro de Desenvolvimento Sustentável, 2011.; Apesar do declínio na incidência e prevalência da cárie dentária, essa doença ainda está bastante presente na população e é o maior problema de saúde bucal. Atualmente, a odontologia de mínima intervenção é o conceito utilizado para o tratamento da cárie. Esforços tem sido realizados no sentido de se obter materiais que possam paralisar as lesões cariosas o mais precocemente possível. A caseína fosfato de cálcio fosopeptídeo amorfo (CPP-ACP), composto derivado da caseína, apresenta potencial remineralizador, impedindo que a lesão cariosa avance. Esse estudo in vivo foi conduzido com o objetivo de avaliar os efeitos da remineralização do CPP-ACP moussenas lesões de mancha branca de cárie em dentes decíduos e permanentes. Dezoito crianças (idade de 4- 8 anos) com 81 lesões de mancha branca foram divididas aleatoriamente em dois protocolos de tratamento: A) grupo teste, que recebeu aplicações da mousse2 vezes por semana, durante 6 semanas; B) grupo controle, que recebeu aplicações de verniz de flúor 2,26% uma vez por semana, durante 4 semanas.Todas as crianças receberam instrução de higiene bucal e recomendações quanto à escovação diária por meio do uso de escova e dentifrício fluoretado...

Estudo in vitro da eficácia anticárie do creme à base do complexo de fosfopeptídeo de caseína (CPP) : fosfato de cálcio amorfo (ACP) em dentes humanos

Pereira, Renata Faria
Fonte: Universidade de Brasília Publicador: Universidade de Brasília
Tipo: Dissertação
POR
Relevância na Pesquisa
27.51%
Dissertação (mestrado)—Universidade de Brasília, Faculdade de Ciências da Saúde, Programa de Pós-Graduação em Ciências da Saúde, 2012.; Introdução: O CPP-ACP já tem demonstrado seu potencial anticárie em estudos in vitro, in situ e in vivo, entretanto notam-se alguns resultados divergentes. Além disso, nem sempre o complexo é comparado e/ou associado ao F, terapia padrão utilizada no controle da cárie. Dentre os estudos que avaliam a eficácia do CPP-ACP, a maioria estuda o seu potencial remineralizador e não a sua capacidade em inibir o processo de desmineralização em lesões iniciais. Objetivo: Avaliar o papel do CPP-ACP (MI Paste) associado ou não ao dentifrício fluoretado (1100 ppm NaF) em inibir a desmineralização dentária por meio de estudo in vitro randomizado. Método: Blocos de esmalte humano, com durezas superficiais iniciais conhecidas foram submetidos a ciclagens de pH por 7 dias, a fim de que se produzissem lesões iniciais de cárie. Durante o período das ciclagens foram administrados os seguintes tratamentos: T1- água deionizada e destilada (controle negativo); T2- CPP-ACP (MI Paste); T3- dentifrício fluoretado (1100 ppm NaF); T4- CPP-ACP (MI Paste) + dentifrício fluoretado (1100 ppm NaF). Posteriormente foi avaliada a dureza longitudinal de todos os blocos. Resultados: Os tratamentos que tiveram melhor desempenho foram os que utilizaram o F isolado ou associado ao CPP-ACP. A perda de dureza observada foi de 6.698...

The effects of 5-HT and m-chlorophenylpiperazine (m-CPP) on the efflux of [3H]-5-HT from human perfused platelets.

Carver, J G; Grahame-Smith, D G; Johnson, E S; Madgwick, Z
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1993 EN
Relevância na Pesquisa
27.29%
1. m-Chlorophenylpiperazine (m-CPP), a 5-HT1c-receptor agonist, induces migraine-like headaches when taken orally by migraine sufferers. The present study was undertaken to see what effects m-CPP had on 5-HT function in platelets. 2. Platelets from healthy male volunteers were loaded with [3H]-5-HT and continuously perfused in vitro with carboxygenated Krebs solution at 37 degrees C. After 30 min washout the effects of m-CPP, thrombin, 5-HT and ADP on the efflux of [3H]-5-HT were recorded. 3. m-CPP (0.5-500 microM) did not evoke an increase in the efflux of [3H]-5-HT over that occurring spontaneously whereas thrombin, unlabelled 5-HT and ADP did. The effects of 5-HT were potentiated by ADP. The results were identical whether or not the 5-HT reuptake blocker paroxetine (1 microM) was present. 4. m-CPP inhibited the increase in the efflux of [3H]-5-HT evoked by different concentrations of unlabelled 5-HT in the presence of ADP (2.5 microM) and displaced the 5-HT log concentration response curve to the right. A similar result was obtained with the 5-HT2-receptor antagonist ketanserin. 5. We conclude that m-CPP is a 5-HT2-receptor antagonist on human platelets, which is unlikely to account for its headache-inducing property, as many drugs effective in migraine prophylaxis have this action.

Effect of chronic m-CPP on locomotion, hypophagia, plasma corticosterone and 5-HT2C receptor levels in the rat

Fone, Kevin C F; Austin, Rachel H; Topham, Ian A; Kennett, Guy A; Punhani, Taniya
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/1998 EN
Relevância na Pesquisa
27.55%
The present study examined 5-HT2C receptor agonist-induced behavioural tolerance and 5-HT2C receptor down-regulation in adult rat brain. The effect of chronic subcutaneous infusion of the 5-HT2C receptor agonist, m-chlorophenylpiperazine (m-CPP, 10 mg kg−1, day−1), for 14 days was examined on daily food intake, the ability of acute m-CPP (2.5 mg kg−1, i.p.) to induce hypolocomotion in a novel arena and elevate plasma corticosterone levels and on ex vivo cortical [3H]-mesulergine binding and hippocampal 5-HT2C receptor protein levels.Before chronic infusion, m-CPP (2.5 mg kg−1, i.p.) attenuated the number of turns and rears made in a novel open field arena. In contrast, while m-CPP still elicited this hypolocomotion following 14 days, saline infusion, no such hypolocomotion occurred in rats given chronic m-CPP (10 mg kg−1 day−1), indicating that almost complete tachyphylaxis of this behaviour occurred with chronic 5-HT2C receptor agonist injection.During chronic infusion of m-CPP, rats consumed less food per day than saline-treated controls. Acute challenge with m-CPP following two weeks, treatment still attenuated food intake over the next four hours (by 43% and 30%, respectively from that on the previous day) in saline and m-CPP infusion groups...

Early-Life Experience Decreases Drug-Induced Reinstatement of Morphine CPP in Adulthood via Microglial-Specific Epigenetic Programming of Anti-Inflammatory IL-10 Expression

Schwarz, Jaclyn M.; Hutchinson, Mark R.; Bilbo, Staci D.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 07/12/2011 EN
Relevância na Pesquisa
27.24%
A critical component of drug addiction research involves identifying novel biological mechanisms and environmental predictors of risk or resilience to drug addiction and associated relapse. Increasing evidence suggests microglia and astrocytes can profoundly affect the physiological and addictive properties of drugs of abuse, including morphine. We report that glia within the rat Nucleus Accumbens (NAcc) respond to morphine with an increase in cytokine/chemokine expression, which predicts future reinstatement of morphine conditioned place preference (CPP) following a priming dose of morphine. This glial response to morphine is influenced by early-life experience. A neonatal handling paradigm that increases the quantity and quality of maternal care significantly increases baseline expression of the anti-inflammatory cytokine IL-10 within the NAcc, attenuates morphine-induced glial activation, and prevents the subsequent reinstatement of morphine CPP in adulthood. IL-10 expression within the NAcc and reinstatement of CPP are negatively correlated, suggesting a protective role for this specific cytokine against morphine-induced glial reactivity and drug-induced reinstatement of morphine CPP. Neonatal handling programs the expression of IL-10 within the NAcc early in development...

Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delivery of antisense oligomers

Nielsen, Peter E.; Shiraishi, Takehiko
Fonte: Landes Bioscience Publicador: Landes Bioscience
Tipo: Artigo de Revista Científica
Publicado em 01/07/2011 EN
Relevância na Pesquisa
27.44%
We have explored the merits of a novel delivery strategy for the antisense oligomers based on cell penetrating peptide (CPP) conjugated to a carrier PNA with sequence complementary to part of the antisense oligomer. The effect of these carrier CPP-PNAs was evaluated by using antisense PNA targeting splicing correction of the mutated luciferase gene in the HeLa pLuc705 cell line, reporting cellular (nuclear) uptake of the antisense PNA via luciferase activity measurement. Carrier CPP-PNA constructs were studied in terms of construct modification (with octaarginine and/or decanoic acid) and carrier PNA length (to adjust binding affinity). In general, the carrier CPP-PNA constructs including the ones with decanoyl modification provided significant increase of the activity of unmodified antisense PNA as well as of antisense octaarginine-PNA conjugates. Antisense activity, and by inference cellular delivery, of unmodified antisense PNA was enhanced at least 20-fold at 6 μM upon the complexation with an equimolar amount of nonamer carrier decanoyl-CPP-PNA (Deca-cPNA1(9)-(D-Arg)8). The antisense activity of a CPP-PNA ((D-Arg)8-asPNA) (at 2 μM) was improved 6-fold and 8-fold by a heptamer carrier CPP-PNA (cPNA1(7)-(D-Arg)8) and hexamer carrier decanoyl-CPP-PNA (Deca-cPNA1(6)-(D-Arg)8)...

Do Not Hallow until You Are out of the Wood! Ultrasonographic Detection of CPP Crystal Deposits in Menisci: Facts and Pitfalls

Filippou, Georgios; Adinolfi, Antonella; Bozios, Panagiotis; Lorenzini, Sauro; Picerno, Valentina; Di Sabatino, Valentina; Bertoldi, Ilaria; Gambera, Dario; Galeazzi, Mauro; Frediani, Bruno
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Publicado em 16/07/2013 EN
Relevância na Pesquisa
27.33%
Purpose. Ultrasonography (US) has been demonstrated to be an important tool in the diagnosis of calcium pyrophosphate (CPP) crystal deposition disease. The aim of our study was to individuate and describe possible pitfalls in US detection of such deposits in menisci. Patients and Methods. We enrolled all patients waiting to undergo knee replacement surgery due to osteoarthritis, for one-month period. Each patient underwent US examination of the knee, focusing on the menisci. After surgery, the menisci were examined by US, macroscopically and microscopically, using the microscopic analysis as the gold standard for CPP deposition. Results. 11 menisci of 6 patients have been studied. Ex vivo examination of menisci performed better in CPP identification than in vivo examination. The possible reasons of misinterpretation or misdiagnosis of the in vivo exam were identified and are extensively described in the paper. Also a new sign of CPP crystal deposits was found. Conclusions. This study permitted to highlight some difficulties in CPP crystal detection by US in menisci. Further studies are needed to define completely US CPP crystal aspect and to improve the sensibility and specificity of US in CPP deposition diagnosis.

Comparative evaluation of the effect of Er:YAG laser and low level laser irradiation combined with CPP-ACPF cream on treatment of enamel caries

Heravi, Farzin; Ahrari, Farzaneh; Mahdavi, Mahdieh; Basafa, Soroush
Fonte: Medicina Oral S.L. Publicador: Medicina Oral S.L.
Tipo: Artigo de Revista Científica
Publicado em 01/04/2014 EN
Relevância na Pesquisa
27.37%
Objectives: This study investigated the effectiveness of low power red and infrared lasers and that of Er:YAG laser, in association with CPP-ACPF cream, on remineralization of white spot lesions. Study Design: Fifty intact premolars were immersed in a demineralization solution for 10 weeks to induce caries like lesions and then were divided into five groups. In group 1, the teeth were covered with a CPP-ACPF cream for 3 minutes and then irradiated with a low power red laser (660 nm, 200 mW) for 1 minute through the cream. In group 2, the treatment was the same as that in group 1, but an infrared laser (810 nm, 200 mW) was employed. The specimens in group 3 were irradiated with an Er:YAG laser (100 mJ, 10 Hz) combined with CPP-ACPF. In group 4, the CPP-ACPF cream was applied for 4 minutes and group 5 was submitted to neither laser nor CPP-ACPF. The micro Vickers hardness was compared at 20, 60 and 100 µ from the enamel surface among the groups. Results: The highest microhardness was observed in the low power red and Er:YAG laser groups and the lowest one belonged to the CPP-ACPF alone and control groups. However, no significant difference was found in microhardness of the experimental groups at any of the evaluation depths (p>0.05). Conclusions: With the laser parameters used in this study...

Measurement of NMDA Receptor Antagonist, CPP, in Mouse Plasma and Brain Tissue Following Systematic Administration Using Ion-Pair LCMS/MS

Gemperline, Erin; Laha, Kurt; Scarlett, Cameron O.; Pearce, Robert A.; Li, Lingjun
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
EN
Relevância na Pesquisa
27.33%
(RS)-3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP) is a competitive antagonist of the N-methyl-D-aspartate (NMDA) receptor and is routinely used with rodent models to investigate the role of NMDA receptors in brain function. This highly polar compound is difficult to separate from biological matrices. A reliable and sensitive assay was developed for the determination of CPP in plasma and tissue. In order to overcome the challenges relating to the physicochemical properties of CPP we employed an initial separation using solid phase extraction harnessing mixed-mode anion exchange. Then an ion-pair UPLC C18 separation was performed followed by MS/MS with a Waters Acquity UPLC interfaced to an AB Sciex QTrap 5500 mass spectrometer, which was operated in positive ion ESI mode. Multiple reaction monitoring (MRM) mode was utilized to detect the analyte and internal standard. The precursor to product ions used for quantitation for CPP and internal standard were m/z 252.958 → 207.100 and 334.955 → 136.033, respectively. This method was applied to a pharmacokinetic study and examined brain tissue and plasma concentrations following intravenous and intraperitoneal injections of CPP. The elimination half-life (t1/2) of CPP was 8.8 minutes in plasma and 14.3 minutes in brain tissue...

The effects of a single exposure to uncontrollable stress on the subsequent conditioned place preference responses to oxycodone, cocaine, and ethanol in rats

Der-Avakian, A.; Bland, S.; Rozeske, R.; Tamblyn, J.; Hutchinson, M.; Watkins, L.; Maier, S.
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
Publicado em //2007 EN
Relevância na Pesquisa
27.24%
Rationale Acute stress has been shown to facilitate the rewarding effects of a number of commonly abused drugs, although the stressor typically must be administered either immediately before or during drug administration and often in the same environment. We have previously reported that a single session of an uncontrollable (inescapable tailshock, IS), but not controllable (escapable tailshock, ES), stressor can enhance the conditioned place preference (CPP) response to morphine, even when stressor and drug administration are separated temporally and spatially. However, this persistent, trans-situational enhancement did not occur to amphetamine CPP. Objectives The following experiments were conducted to determine whether the long-term effects of IS on drug reward are specific to opioids. Materials and methods Adult, male Sprague–Dawley rats were exposed to a single session of IS or remained in their home cages (HC). Twenty-four hours later, using an unbiased procedure, CPP conditioning was conducted with either oxycodone (0, 2, or 5 mg/kg, sc), cocaine (0, 1, 5, or 10 mg/kg, ip), or ethanol (0.3, 1, or 2 g/kg, ip). Another group of rats were exposed to IS, ES, or HC treatment and conditioned with oxycodone (5 mg/kg, sc) 24 h later. Results IS enhanced the subsequent CPP response to oxycodone...

The effect of CPP-ACP on enamel wear under severe erosive conditions

Ranjitkar, S.; Kaidonis, J.; Richards, L.; Townsend, G.
Fonte: Pergamon-Elsevier Science Ltd Publicador: Pergamon-Elsevier Science Ltd
Tipo: Artigo de Revista Científica
Publicado em //2009 EN
Relevância na Pesquisa
27.24%
OBJECTIVE: In addition to its role as a remineralizing agent in preventing dental caries, recent evidence has shown that casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) can protect teeth against erosion. The aim of this study was to determine whether CPP-ACP could reduce enamel wear rates under severe erosive conditions simulating heavy attrition and gastric regurgitation. DESIGN: Enamel specimens were subjected to 10,000 wear cycles at a load of 100 N and pH 1.2 in a tooth wear machine. The machine was stopped every 2 min (160 cycles), and CPP-ACP in the form of a paste was applied for 5 min in experimental group 1. A paste with the same formulation but without CPP-ACP was applied in experimental group 2. No paste was applied in the control group. RESULTS: A linear mixed model analysis indicated that the mean wear rates in experimental group 1 (0.44+/-0.05 mm(3) per 1000 cycles) and in experimental group 2 (0.63+/-0.06 mm(3) per 1000 cycles) were significantly lower than that in the control group (0.92+/-0.11 mm(3) per 1000 cycles) (p<0.05). The mean wear rate in experimental group 1 was also lower than that in experimental group 2 (p<0.05). Wear facets in experimental groups 1 and 2 were noted to be smoother and more polished than those in the control group. CONCLUSIONS: Both remineralizing and lubricating properties of the paste containing CPP-ACP appear to contribute to wear reduction in enamel. These findings may lead to new strategies for the clinical management of tooth wear.; http://www.elsevier.com/wps/find/journaldescription.cws_home/203/description#description; Sarbin Ranjitkar...

Biology of tooth wear: preventive strategies.

Ranjitkar, Sarbin
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2010
Relevância na Pesquisa
27.44%
There is an increasing awareness in clinical dentistry of the need to better understand the aetiology and management of tooth wear, as increasing numbers of elderly patients are retaining their natural teeth to a stage when they present with extensive wear. In addition, more younger patients are presenting with wear of both primary and permanent teeth. In order to comply with the philosophy of minimal intervention dentistry, clinical management of tooth wear should focus on early detection and prevention before a restorative approach is considered. Fluoride is a commonly used agent in the management of tooth wear, with previous studies showing that it can protect teeth against erosion (at around pH 2.0 and 3.0) and toothbrush abrasion in an acidic environment (at around pH 3.0). However, it does not reduce attritional wear between opposing tooth specimens. Nightguards are commonly used to prevent attritional wear but there is still a need to improve preventive strategies to manage the risk of erosive tooth wear. Previous in vitro studies have shown that a remineralizing agent in the form of CPP-ACP (casein phosphopeptide – amorphous calcium phosphate) can reduce erosion of both enamel and dentine by white wine. A recent study has also highlighted the potential lubricating and remineralizing properties of a CPP-ACP containing paste (Tooth Mousse®...

Early-life experience decreases drug-induced reinstatement of morphine CPP in adulthood via microglial-specific epigenetic programming of anti-inflammatory IL-10 expression

Schwarz, J.; Hutchinson, M.; Bilbo, S.
Fonte: Soc Neuroscience Publicador: Soc Neuroscience
Tipo: Artigo de Revista Científica
Publicado em //2011 EN
Relevância na Pesquisa
27.24%
A critical component of drug addiction research involves identifying novel biological mechanisms and environmental predictors of risk or resilience to drug addiction and associated relapse. Increasing evidence suggests microglia and astrocytes can profoundly affect the physiological and addictive properties of drugs of abuse, including morphine. We report that glia within the rat nucleus accumbens (NAcc) respond to morphine with an increase in cytokine/chemokine expression, which predicts future reinstatement of morphine conditioned place preference (CPP) following a priming dose of morphine. This glial response to morphine is influenced by early-life experience. A neonatal handling paradigm that increases the quantity and quality of maternal care significantly increases baseline expression of the anti-inflammatory cytokine IL-10 within the NAcc, attenuates morphine-induced glial activation, and prevents the subsequent reinstatement of morphine CPP in adulthood. IL-10 expression within the NAcc and reinstatement of CPP are negatively correlated, suggesting a protective role for this specific cytokine against morphine-induced glial reactivity and drug-induced reinstatement of morphine CPP. Neonatal handling programs the expression of IL-10 within the NAcc early in development...

Associação do laser diodo com CPP-ACP/ verniz fluoretado na resistência a desmineralização do esmalte dental; Effect of diode laser associated with CPP-ACP/ fluoride varnish on demineralization of dental enamel

Azevedo, Danielle Torres
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 07/08/2014 PT
Relevância na Pesquisa
37.37%
O objetivo do presente estudo foi avaliar o efeito da associação do laser de diodo ao CPP-ACP ou verniz fluoretado na resistência a desmineralização do esmalte dentário. Foram utilizados 90 fragmentos de terceiros molares humanos irrompidos, selecionados por microdureza superficial e isolados com resina composta deixando apenas uma área de 4x4mm exposta. Em seguida foram desmineralizados por 32 h e 60 espécimes tiveram metade da área (2x4mm) isolada. Os espécimes foram divididos aleatoriamente em seis grupos/subgrupos, de acordo com os tratamentos propostos: G1-Controle (sem tratamento); G2- CPP-ACP, G3-verniz fluoretado Duraphat®; A - sem laser, B- tratamento com laser de diodo (0,7W, 70mJ, 10Hz). Na sequência foi realizado ciclo de pH para indução de desmineralização por oito dias. A solução desmineralizante de cada espécime foi armazenada para análise do cálcio por espectrometria de absorção atômica (n=10). Os espécimes foram seccionados ao meio, uma das metades foi analisada por microdureza longitudinal (n=10) e a outra por rugosidade (n=10) e morfologia superficial (n=10). Para a análise por Tomografia de Coerência Óptica (OCT) foi empregado n=5 por grupo/subgrupo e realizou-se a OCT em 2 fases, sendo a primeira após a desmineralização por 32h e a segunda após os tratamento/ciclagem de pH. Para a microdureza empregou-se a variação percentual da dureza...

CPP Magnetoresistance of Magnetic Multilayers: A critical review

Bass, Jack
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 23/11/2015
Relevância na Pesquisa
27.33%
We present a comprehensive review of data and analysis of Giant (G) Magnetoresistance (MR) with Current-flow Perpendicular-to-layer-Planes (CPP-MR) of magnetic multilayers [F/N]n (n = number of repeats) with alternating nanoscale layers of ferromagnetic (F) and non-magnetic (N) metals. GMR, a large change in resistance when an applied magnetic field changes the moment ordering of adjacent F-layers from anti-parallel (AP) to parallel (P), was discovered in 1988 in the Current-flow-in-layer-Planes (CIP) geometry. The CPP-MR has two advantages over the CIP-MR: (1) it allows more direct access to the underlying physics; and (2) it is usually larger, which should be advantageous for devices. When the first CPP-MR data were published in 1991, it was not clear whether electronic transport in GMR multilayers is fully diffusive or at least partly ballistic. It was not known whether the properties of layers and interfaces would vary with layer thickness or number. It was not known if the CPP-MR would be dominated by scattering within the F-metals or at the F/N interfaces. Nothing was known about: (1) spin-flipping within F-metals, characterized by a spin-diffusion length, l(F)sf; (2) interface specific resistances (AR = area A times resistance R) for N1/N2 interfaces; (3) interface specific resistances and spin-dependent scattering asymmetry at F/N and F1/F2 interfaces; and (4) spin-flipping at F/N...